TRMU cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRMU
Entrez Gene ID	55687
Full Name	tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase
Synonyms	LCAL3,MTO2,MTU1,TRMT,TRMT1
General protein information	Preferred Names tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase Names mitochondrial tRNA-specific 2-thiouridylase 1 MTO2 homolog lung cancer associated lncRNA 3 mitochondrial 5-methylaminomethyl-2-thiouridylate-methyltransferase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q13.31
Summary	This nuclear gene encodes a mitochondrial tRNA-modifying enzyme. The encoded protein catalyzes the 2-thiolation of uridine on the wobble positions of tRNA(Lys), tRNA(Glu), and tRNA(Gln), resulting in the formation of 5-taurinomethyl-2-thiouridine moieties. Mutations in this gene may cause transient infantile liver failure. Polymorphisms in this gene may also influence the severity of deafness caused by mitochondrial 12S ribosomal RNA mutations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013].
Disorder MIM:	610230
Disorder Html:	{Deafness, mitochondrial, modifier of}, 580000 (3); Liver failure, transient infantile, 613070 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001282785.1	NP_001269714.1	mitochondrial tRNA-specific 2-thiouridylase 1 isoform g
NM_018006.5	NP_060476.2	mitochondrial tRNA-specific 2-thiouridylase 1 isoform a
XM_011530271.2	XP_011528573.1	mitochondrial tRNA-specific 2-thiouridylase 1 isoform X1
XM_011530272.1	XP_011528574.1	mitochondrial tRNA-specific 2-thiouridylase 1 isoform X2
XM_011530273.1	XP_011528575.1	mitochondrial tRNA-specific 2-thiouridylase 1 isoform X3
XM_011530274.2	XP_011528576.1	mitochondrial tRNA-specific 2-thiouridylase 1 isoform X5
XM_005261678.1	XP_005261735.1	mitochondrial tRNA-specific 2-thiouridylase 1 isoform X6
XM_005261681.1	XP_005261738.1	mitochondrial tRNA-specific 2-thiouridylase 1 isoform X7
NM_018006.4	NP_060476.2	mitochondrial tRNA-specific 2-thiouridylase 1 isoform a
NM_001282784.1	NP_001269713.1	mitochondrial tRNA-specific 2-thiouridylase 1 isoform f
NM_001282783.1	NP_001269712.1	mitochondrial tRNA-specific 2-thiouridylase 1 isoform e
XM_011530272.2	XP_011528574.1	mitochondrial tRNA-specific 2-thiouridylase 1 isoform X2
XM_011530273.2	XP_011528575.1	mitochondrial tRNA-specific 2-thiouridylase 1 isoform X3
NM_001282783.2	NP_001269712.1	mitochondrial tRNA-specific 2-thiouridylase 1 isoform e
NM_001282782.2	NP_001269711.1	mitochondrial tRNA-specific 2-thiouridylase 1 isoform d
XM_024452260.1	XP_024308028.1	mitochondrial tRNA-specific 2-thiouridylase 1 isoform X4
NM_001282785.2	NP_001269714.1	mitochondrial tRNA-specific 2-thiouridylase 1 isoform g
NM_001282782.1	NP_001269711.1	mitochondrial tRNA-specific 2-thiouridylase 1 isoform d
NM_001282784.2	NP_001269713.1	mitochondrial tRNA-specific 2-thiouridylase 1 isoform f
XM_011530275.1	XP_011528577.1	mitochondrial tRNA-specific 2-thiouridylase 1 isoform X6

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRMU	NP_060476.2
Mus musculus (house mouse)	Trmu	NP_082339.1
Schizosaccharomyces pombe (fission yeast)	SPAC23H4.04	NP_593402.1
Oryza sativa (rice)	Os03g0174600	NP_001049129.1
Drosophila melanogaster (fruit fly)	CG3021	NP_652639.1
Caenorhabditis elegans (roundworm)	B0035.16	NP_502120.1
Kluyveromyces lactis	KLLA0F24706g	XP_456180.1
Arabidopsis thaliana (thale cress)	AT1G51310	NP_175542.2
Pan troglodytes (chimpanzee)	TRMU	XP_515202.3
	TRMU	XP_001111019.1
Rattus norvegicus (Norway rat)	Trmu	NP_001129348.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011960	XP_320574.3
Saccharomyces cerevisiae (baker's yeast)	SLM3	NP_010251.1
Eremothecium gossypii	AGOS_AEL087C	NP_984774.2
Canis lupus familiaris (dog)	TRMU	XP_538325.2
Bos taurus (cattle)	TRMU	NP_001156660.1
Gallus gallus (chicken)	TRMU	NP_001026522.1
Danio rerio (zebrafish)	trmu	NP_001018435.1
Xenopus tropicalis (tropical clawed frog)	trmu	XP_002932238.1

Gene Ontology
Bibliography

Related articles in PubMed

Biochemical Evidence for a Nuclear Modifier Allele (A10S) in TRMU (Methylaminomethyl-2-thiouridylate-methyltransferase) Related to Mitochondrial tRNA Modification in the Phenotypic Manifestation of Deafness-associated 12S rRNA Mutation.
Meng F, Cang X, Peng Y, Li R, Zhang Z, Li F, Fan Q, Guan AS, Fischel-Ghosian N, Zhao X, Guan MX
The Journal of biological chemistry292(7)2881-2892(2017 02)

The ROS-sensitive microRNA-9/9* controls the expression of mitochondrial tRNA-modifying enzymes and is involved in the molecular mechanism of MELAS syndrome.
Meseguer S, Mart?nez-Zamora A, Garc?a-Arum? E, Andreu AL, Armengod ME
Human molecular genetics24(1)167-84(2015 Jan)

Nuclear factors: roles related to mitochondrial deafness.
Luo LF, Hou CC, Yang WX
Gene520(2)79-89(2013 May)

A novel mutation in YARS2 causes myopathy with lactic acidosis and sideroblastic anemia.
Sasarman F, Nishimura T, Thiffault I, Shoubridge EA
Human mutation33(8)1201-6(2012 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The A10S mutation caused marked decreases in 2-thiouridine modification of U34 of tRNA(Lys), tRNA(Glu) and tRNA(Gln) However, the A10S mutation mildly increased the aminoacylated efficiency of tRNAs
Title: Biochemical Evidence for a Nuclear Modifier Allele (A10S) in TRMU (Methylaminomethyl-2-thiouridylate-methyltransferase) Related to Mitochondrial tRNA Modification in the Phenotypic Manifestation of Deafness-associated 12S rRNA Mutation.

Results show that post-transcriptional expression of GTPBP3, MTO1 and TRMU genes is down-regulated, leading to mt-tRNA hypomodification and contributing to mitochondrial dysfunction in MELAS cybrids.
Title: The ROS-sensitive microRNA-9/9* controls the expression of mitochondrial tRNA-modifying enzymes and is involved in the molecular mechanism of MELAS syndrome.

An additional, heterozygous mutation was detected in TRMU/MTU1. Although subject myoblasts and myotubes contained half the normal levels of TRMU, thiolation of mitochondrial tRNAs was normal.
Title: A novel mutation in YARS2 causes myopathy with lactic acidosis and sideroblastic anemia.

MTU1 is not required for mitochondrial translation.
Title: The 2-thiouridylase function of the human MTU1 (TRMU) enzyme is dispensable for mitochondrial translation.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

The following TRMU gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRMU cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu08777	NM_001282783.2 Latest version!	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu00544	NM_001282782.2 Latest version!	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu07908	NM_001282784.2 Latest version!	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu10076	NM_001282785.2 Latest version!	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu19683	NM_018006.5 Latest version!	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$244.30 $349.00
OHu00544	XM_011530274.2 Latest version!	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu53634	XM_011530275.1 Latest version!	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu53634	XM_005261678.1 Latest version!	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu53635	XM_005261681.1 Latest version!	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu69627	XM_011530271.2 Latest version!	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu69628	XM_011530272.2 Latest version!	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu69629	XM_011530273.2 Latest version!	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu105271	XM_024452260.1 Latest version!	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu19683	NM_018006.4	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$244.30 $349.00
OHu69628	XM_011530272.1	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu69629	XM_011530273.1	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu08777	NM_001282783.1	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu10076	NM_001282785.1	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu00544	NM_001282782.1	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu07908	NM_001282784.1	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRMU ( NM_001282783.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269712.1 Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu08777
Clone ID Related Accession (Same CDS sequence)	NM_001282783.1 , NM_001282783.2
Accession Version	NM_001282783.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	mitochondrial tRNA-specific 2-thiouridylase 1 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN997542.1, AY062123.2, BC027991.1, AL031588.1 and BC066593.1. On Sep 19, 2013 this sequence version replaced XM_005261680.1. Transcript Variant: This variant (5) contains multiple differences in the coding region, including initiation of translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (e) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC027991.1, SRR1163655.650259.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCATTGC CACAGGTCAC TATGCAAGAA CTTCCCTGGA AGATGAAGAA GTCTTTGAGC 
AGAAGCACGT TAAGAAGCCC GAAGGGCTTT TCAGAAATCG GTTTGAAGTT AGAAATGGTT 
TCCCAGGATG CCCTGAGGAG AACCATCTTC CCTCTGGGGG GATTAACGAA AGAGTTTGTA 
AAGAAAATCG CTGCTGAGAA TAGACTTCAT CATGTGCTTC AGAAGAAAGA GAGCATGGGC 
ATGTGTTTCA TCGGGAAGAG GAATTTTGAA CATTTCCTTC TTCAGTATCT GCAGCCTCGA 
CCTGGTCACT TTATTTCCAT AGAAGACAAT AAGGTTCTGG GAACACATAA AGGTTGGTTC 
CTGTATACCT TGGGCCAGAG AGCAAACATA GGTGGCCTGA GAGAGCCCTG GTACGTGGTG 
GAGAAGGACA GCGTCAAGGG TGACGTGTTT GTGGCCCCCC GGACAGACCA CCCAGCCCTG 
TACAGGGACC TGCTGAGGAC CAGCCGCGTG CACTGGATTG CGGAGGAGCC TCCCGCAGCA 
CTGGTCCGGG ACAAGATGAT GGAGTGCCAC TTCCGATTCC GCCACCAGAT GGCACTAGTG 
CCCTGTGTGC TGACCCTCAA TCAAGATGGC ACCGTGTGGG TGACAGCTGT GCAGGCTGTG 
CGTGCCCTTG CCACAGGACA GTTTGCTGTG TTCTACAAGG GGGACGAGTG CCTGGGCAGC 
GGGAAGATCC TGCGGCTGGG GCCGTCTGCC TACACGCTCC AGAAGGGCCA GCGCAGAGCT 
GGGATGGCCA CTGAGAGCCC CAGTGACAGC CCAGAAGATG GTCCAGGCCT GAGTCCCTTG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269712.1
CDS	619..1464
Translation	MPLPQVTMQELPWKMKKSLSRSTLRSPKGFSEIGLKLEMVSQDALRRTIFPLGGLTKEFVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHFISIEDNKVLGTHKGWFLYTLGQRANIGGLREPWYVVEKDSVKGDVFVAPRTDHPALYRDLLRTSRVHWIAEEPPAALVRDKMMECHFRFRHQMALVPCVLTLNQDGTVWVTAVQAVRALATGQFAVFYKGDECLGSGKILRLGPSAYTLQKGQRRAGMATESPSDSPEDGPGLSPLL

Target ORF information:

RefSeq Version	NM_001282783.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282783.1

ORF Insert Sequence:

ATGCCATTGC CACAGGTCAC TATGCAAGAA CTTCCCTGGA AGATGAAGAA GTCTTTGAGC 
AGAAGCACGT TAAGAAGCCC GAAGGGCTTT TCAGAAATCG GTTTGAAGTT AGAAATGGTT 
TCCCAGGATG CCCTGAGGAG AACCATCTTC CCTCTGGGGG GATTAACGAA AGAGTTTGTA 
AAGAAAATCG CTGCTGAGAA TAGACTTCAT CATGTGCTTC AGAAGAAAGA GAGCATGGGC 
ATGTGTTTCA TCGGGAAGAG GAATTTTGAA CATTTCCTTC TTCAGTATCT GCAGCCTCGA 
CCTGGTCACT TTATTTCCAT AGAAGACAAT AAGGTTCTGG GAACACATAA AGGTTGGTTC 
CTGTATACCT TGGGCCAGAG AGCAAACATA GGTGGCCTGA GAGAGCCCTG GTACGTGGTG 
GAGAAGGACA GCGTCAAGGG TGACGTGTTT GTGGCCCCCC GGACAGACCA CCCAGCCCTG 
TACAGGGACC TGCTGAGGAC CAGCCGCGTG CACTGGATTG CGGAGGAGCC TCCCGCAGCA 
CTGGTCCGGG ACAAGATGAT GGAGTGCCAC TTCCGATTCC GCCACCAGAT GGCACTAGTG 
CCCTGTGTGC TGACCCTCAA TCAAGATGGC ACCGTGTGGG TGACAGCTGT GCAGGCTGTG 
CGTGCCCTTG CCACAGGACA GTTTGCTGTG TTCTACAAGG GGGACGAGTG CCTGGGCAGC 
GGGAAGATCC TGCGGCTGGG GCCGTCTGCC TACACGCTCC AGAAGGGCCA GCGCAGAGCT 
GGGATGGCCA CTGAGAGCCC CAGTGACAGC CCAGAAGATG GTCCAGGCCT GAGTCCCTTG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMU ( NM_001282783.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269712.1 Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu08777
Clone ID Related Accession (Same CDS sequence)	NM_001282783.1 , NM_001282783.2
Accession Version	NM_001282783.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-20
Organism	Homo sapiens(human)
Product	mitochondrial tRNA-specific 2-thiouridylase 1 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY062123.2, BC027991.1, AL031588.1 and BC066593.1. On Jun 1, 2019 this sequence version replaced NM_001282783.1. Transcript Variant: This variant (5) contains multiple differences in the coding region, including initiation of translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (e) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC027991.1, SRR3476690.968487.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCATTGC CACAGGTCAC TATGCAAGAA CTTCCCTGGA AGATGAAGAA GTCTTTGAGC 
AGAAGCACGT TAAGAAGCCC GAAGGGCTTT TCAGAAATCG GTTTGAAGTT AGAAATGGTT 
TCCCAGGATG CCCTGAGGAG AACCATCTTC CCTCTGGGGG GATTAACGAA AGAGTTTGTA 
AAGAAAATCG CTGCTGAGAA TAGACTTCAT CATGTGCTTC AGAAGAAAGA GAGCATGGGC 
ATGTGTTTCA TCGGGAAGAG GAATTTTGAA CATTTCCTTC TTCAGTATCT GCAGCCTCGA 
CCTGGTCACT TTATTTCCAT AGAAGACAAT AAGGTTCTGG GAACACATAA AGGTTGGTTC 
CTGTATACCT TGGGCCAGAG AGCAAACATA GGTGGCCTGA GAGAGCCCTG GTACGTGGTG 
GAGAAGGACA GCGTCAAGGG TGACGTGTTT GTGGCCCCCC GGACAGACCA CCCAGCCCTG 
TACAGGGACC TGCTGAGGAC CAGCCGCGTG CACTGGATTG CGGAGGAGCC TCCCGCAGCA 
CTGGTCCGGG ACAAGATGAT GGAGTGCCAC TTCCGATTCC GCCACCAGAT GGCACTAGTG 
CCCTGTGTGC TGACCCTCAA TCAAGATGGC ACCGTGTGGG TGACAGCTGT GCAGGCTGTG 
CGTGCCCTTG CCACAGGACA GTTTGCTGTG TTCTACAAGG GGGACGAGTG CCTGGGCAGC 
GGGAAGATCC TGCGGCTGGG GCCGTCTGCC TACACGCTCC AGAAGGGCCA GCGCAGAGCT 
GGGATGGCCA CTGAGAGCCC CAGTGACAGC CCAGAAGATG GTCCAGGCCT GAGTCCCTTG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269712.1
CDS	306..1151
Translation	MPLPQVTMQELPWKMKKSLSRSTLRSPKGFSEIGLKLEMVSQDALRRTIFPLGGLTKEFVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHFISIEDNKVLGTHKGWFLYTLGQRANIGGLREPWYVVEKDSVKGDVFVAPRTDHPALYRDLLRTSRVHWIAEEPPAALVRDKMMECHFRFRHQMALVPCVLTLNQDGTVWVTAVQAVRALATGQFAVFYKGDECLGSGKILRLGPSAYTLQKGQRRAGMATESPSDSPEDGPGLSPLL

Target ORF information:

RefSeq Version	NM_001282783.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282783.2

ORF Insert Sequence:

ATGCCATTGC CACAGGTCAC TATGCAAGAA CTTCCCTGGA AGATGAAGAA GTCTTTGAGC 
AGAAGCACGT TAAGAAGCCC GAAGGGCTTT TCAGAAATCG GTTTGAAGTT AGAAATGGTT 
TCCCAGGATG CCCTGAGGAG AACCATCTTC CCTCTGGGGG GATTAACGAA AGAGTTTGTA 
AAGAAAATCG CTGCTGAGAA TAGACTTCAT CATGTGCTTC AGAAGAAAGA GAGCATGGGC 
ATGTGTTTCA TCGGGAAGAG GAATTTTGAA CATTTCCTTC TTCAGTATCT GCAGCCTCGA 
CCTGGTCACT TTATTTCCAT AGAAGACAAT AAGGTTCTGG GAACACATAA AGGTTGGTTC 
CTGTATACCT TGGGCCAGAG AGCAAACATA GGTGGCCTGA GAGAGCCCTG GTACGTGGTG 
GAGAAGGACA GCGTCAAGGG TGACGTGTTT GTGGCCCCCC GGACAGACCA CCCAGCCCTG 
TACAGGGACC TGCTGAGGAC CAGCCGCGTG CACTGGATTG CGGAGGAGCC TCCCGCAGCA 
CTGGTCCGGG ACAAGATGAT GGAGTGCCAC TTCCGATTCC GCCACCAGAT GGCACTAGTG 
CCCTGTGTGC TGACCCTCAA TCAAGATGGC ACCGTGTGGG TGACAGCTGT GCAGGCTGTG 
CGTGCCCTTG CCACAGGACA GTTTGCTGTG TTCTACAAGG GGGACGAGTG CCTGGGCAGC 
GGGAAGATCC TGCGGCTGGG GCCGTCTGCC TACACGCTCC AGAAGGGCCA GCGCAGAGCT 
GGGATGGCCA CTGAGAGCCC CAGTGACAGC CCAGAAGATG GTCCAGGCCT GAGTCCCTTG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMU ( NM_001282782.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269711.1 Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu00544
Clone ID Related Accession (Same CDS sequence)	XM_011530274.2 , NM_001282782.2 , NM_001282782.1
Accession Version	NM_001282782.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-20
Organism	Homo sapiens(human)
Product	mitochondrial tRNA-specific 2-thiouridylase 1 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ309999.1 and BC066593.1. On May 31, 2019 this sequence version replaced NM_001282782.1. Transcript Variant: This variant (3) lacks an alternate exon in the 5' coding region and initiates translation at an alternate downstream start codon, compared to variant 1. The encoded isoform (d) is shorter and has a distinct N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ309999.1, BC012076.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATGTGTT CAGGGGCAGA TGCCATTGCC ACAGGTCACT ATGCAAGAAC TTCCCTGGAA 
GATGAAGAAG TCTTTGAGCA GAAGCACGTT AAGAAGCCCG AAGGGCTTTT CAGAAATCGG 
TTTGAAGTTA GAAATGCGGT AAAACTCCTC CAGGCAGCTG ACAGCTTTAA AGACCAGACC 
TTCTTTCTCA GCCAGGTTTC CCAGGATGCC CTGAGGAGAA CCATCTTCCC TCTGGGGGGA 
TTAACGAAAG AGTTTGTAAA GAAAATCGCT GCTGAGAATA GACTTCATCA TGTGCTTCAG 
AAGAAAGAGA GCATGGGCAT GTGTTTCATC GGGAAGAGGA ATTTTGAACA TTTCCTTCTT 
CAGTATCTGC AGCCTCGACC TGGTCACTTT ATTTCCATAG AAGACAATAA GGTTCTGGGA 
ACACATAAAG GTTGGTTCCT GTATACCTTG GGCCAGAGAG CAAACATAGG TGGCCTGAGA 
GAGCCCTGGT ACGTGGTGGA GAAGGACAGC GTCAAGGGTG ACGTGTTTGT GGCCCCCCGG 
ACAGACCACC CAGCCCTGTA CAGGGACCTG CTGAGGACCA GCCGCGTGCA CTGGATTGCG 
GAGGAGCCTC CCGCAGCACT GGTCCGGGAC AAGATGATGG AGTGCCACTT CCGATTCCGC 
CACCAGATGG CACTAGTGCC CTGTGTGCTG ACCCTCAATC AAGATGGCAC CGTGTGGGTG 
ACAGCTGTGC AGGCTGTGCG TGCCCTTGCC ACAGGACAGT TTGCTGTGTT CTACAAGGGG 
GACGAGTGCC TGGGCAGCGG GAAGATCCTG CGGCTGGGGC CGTCTGCCTA CACGCTCCAG 
AAGGGCCAGC GCAGAGCTGG GATGGCCACT GAGAGCCCCA GTGACAGCCC AGAAGATGGT 
CCAGGCCTGA GTCCCTTGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269711.1
CDS	287..1210
Translation	MMCSGADAIATGHYARTSLEDEEVFEQKHVKKPEGLFRNRFEVRNAVKLLQAADSFKDQTFFLSQVSQDALRRTIFPLGGLTKEFVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHFISIEDNKVLGTHKGWFLYTLGQRANIGGLREPWYVVEKDSVKGDVFVAPRTDHPALYRDLLRTSRVHWIAEEPPAALVRDKMMECHFRFRHQMALVPCVLTLNQDGTVWVTAVQAVRALATGQFAVFYKGDECLGSGKILRLGPSAYTLQKGQRRAGMATESPSDSPEDGPGLSPLL

Target ORF information:

RefSeq Version	NM_001282782.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282782.2

ORF Insert Sequence:

ATGATGTGTT CAGGGGCAGA TGCCATTGCC ACAGGTCACT ATGCAAGAAC TTCCCTGGAA 
GATGAAGAAG TCTTTGAGCA GAAGCACGTT AAGAAGCCCG AAGGGCTTTT CAGAAATCGG 
TTTGAAGTTA GAAATGCGGT AAAACTCCTC CAGGCAGCTG ACAGCTTTAA AGACCAGACC 
TTCTTTCTCA GCCAGGTTTC CCAGGATGCC CTGAGGAGAA CCATCTTCCC TCTGGGGGGA 
TTAACGAAAG AGTTTGTAAA GAAAATCGCT GCTGAGAATA GACTTCATCA TGTGCTTCAG 
AAGAAAGAGA GCATGGGCAT GTGTTTCATC GGGAAGAGGA ATTTTGAACA TTTCCTTCTT 
CAGTATCTGC AGCCTCGACC TGGTCACTTT ATTTCCATAG AAGACAATAA GGTTCTGGGA 
ACACATAAAG GTTGGTTCCT GTATACCTTG GGCCAGAGAG CAAACATAGG TGGCCTGAGA 
GAGCCCTGGT ACGTGGTGGA GAAGGACAGC GTCAAGGGTG ACGTGTTTGT GGCCCCCCGG 
ACAGACCACC CAGCCCTGTA CAGGGACCTG CTGAGGACCA GCCGCGTGCA CTGGATTGCG 
GAGGAGCCTC CCGCAGCACT GGTCCGGGAC AAGATGATGG AGTGCCACTT CCGATTCCGC 
CACCAGATGG CACTAGTGCC CTGTGTGCTG ACCCTCAATC AAGATGGCAC CGTGTGGGTG 
ACAGCTGTGC AGGCTGTGCG TGCCCTTGCC ACAGGACAGT TTGCTGTGTT CTACAAGGGG 
GACGAGTGCC TGGGCAGCGG GAAGATCCTG CGGCTGGGGC CGTCTGCCTA CACGCTCCAG 
AAGGGCCAGC GCAGAGCTGG GATGGCCACT GAGAGCCCCA GTGACAGCCC AGAAGATGGT 
CCAGGCCTGA GTCCCTTGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMU ( NM_001282782.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269711.1 Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu00544
Clone ID Related Accession (Same CDS sequence)	XM_011530274.2 , NM_001282782.2 , NM_001282782.1
Accession Version	NM_001282782.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	mitochondrial tRNA-specific 2-thiouridylase 1 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN997542.1, DQ309999.1 and BC066593.1. On Sep 19, 2013 this sequence version replaced XM_005261677.1. Transcript Variant: This variant (3) lacks an alternate exon in the 5' coding region and initiates translation at an alternate downstream start codon, compared to variant 1. The encoded isoform (d) is shorter and has a distinct N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ309999.1, SRR1163658.210810.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATGTGTT CAGGGGCAGA TGCCATTGCC ACAGGTCACT ATGCAAGAAC TTCCCTGGAA 
GATGAAGAAG TCTTTGAGCA GAAGCACGTT AAGAAGCCCG AAGGGCTTTT CAGAAATCGG 
TTTGAAGTTA GAAATGCGGT AAAACTCCTC CAGGCAGCTG ACAGCTTTAA AGACCAGACC 
TTCTTTCTCA GCCAGGTTTC CCAGGATGCC CTGAGGAGAA CCATCTTCCC TCTGGGGGGA 
TTAACGAAAG AGTTTGTAAA GAAAATCGCT GCTGAGAATA GACTTCATCA TGTGCTTCAG 
AAGAAAGAGA GCATGGGCAT GTGTTTCATC GGGAAGAGGA ATTTTGAACA TTTCCTTCTT 
CAGTATCTGC AGCCTCGACC TGGTCACTTT ATTTCCATAG AAGACAATAA GGTTCTGGGA 
ACACATAAAG GTTGGTTCCT GTATACCTTG GGCCAGAGAG CAAACATAGG TGGCCTGAGA 
GAGCCCTGGT ACGTGGTGGA GAAGGACAGC GTCAAGGGTG ACGTGTTTGT GGCCCCCCGG 
ACAGACCACC CAGCCCTGTA CAGGGACCTG CTGAGGACCA GCCGCGTGCA CTGGATTGCG 
GAGGAGCCTC CCGCAGCACT GGTCCGGGAC AAGATGATGG AGTGCCACTT CCGATTCCGC 
CACCAGATGG CACTAGTGCC CTGTGTGCTG ACCCTCAATC AAGATGGCAC CGTGTGGGTG 
ACAGCTGTGC AGGCTGTGCG TGCCCTTGCC ACAGGACAGT TTGCTGTGTT CTACAAGGGG 
GACGAGTGCC TGGGCAGCGG GAAGATCCTG CGGCTGGGGC CGTCTGCCTA CACGCTCCAG 
AAGGGCCAGC GCAGAGCTGG GATGGCCACT GAGAGCCCCA GTGACAGCCC AGAAGATGGT 
CCAGGCCTGA GTCCCTTGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269711.1
CDS	600..1523
Translation	MMCSGADAIATGHYARTSLEDEEVFEQKHVKKPEGLFRNRFEVRNAVKLLQAADSFKDQTFFLSQVSQDALRRTIFPLGGLTKEFVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHFISIEDNKVLGTHKGWFLYTLGQRANIGGLREPWYVVEKDSVKGDVFVAPRTDHPALYRDLLRTSRVHWIAEEPPAALVRDKMMECHFRFRHQMALVPCVLTLNQDGTVWVTAVQAVRALATGQFAVFYKGDECLGSGKILRLGPSAYTLQKGQRRAGMATESPSDSPEDGPGLSPLL

Target ORF information:

RefSeq Version	NM_001282782.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282782.1

ORF Insert Sequence:

ATGATGTGTT CAGGGGCAGA TGCCATTGCC ACAGGTCACT ATGCAAGAAC TTCCCTGGAA 
GATGAAGAAG TCTTTGAGCA GAAGCACGTT AAGAAGCCCG AAGGGCTTTT CAGAAATCGG 
TTTGAAGTTA GAAATGCGGT AAAACTCCTC CAGGCAGCTG ACAGCTTTAA AGACCAGACC 
TTCTTTCTCA GCCAGGTTTC CCAGGATGCC CTGAGGAGAA CCATCTTCCC TCTGGGGGGA 
TTAACGAAAG AGTTTGTAAA GAAAATCGCT GCTGAGAATA GACTTCATCA TGTGCTTCAG 
AAGAAAGAGA GCATGGGCAT GTGTTTCATC GGGAAGAGGA ATTTTGAACA TTTCCTTCTT 
CAGTATCTGC AGCCTCGACC TGGTCACTTT ATTTCCATAG AAGACAATAA GGTTCTGGGA 
ACACATAAAG GTTGGTTCCT GTATACCTTG GGCCAGAGAG CAAACATAGG TGGCCTGAGA 
GAGCCCTGGT ACGTGGTGGA GAAGGACAGC GTCAAGGGTG ACGTGTTTGT GGCCCCCCGG 
ACAGACCACC CAGCCCTGTA CAGGGACCTG CTGAGGACCA GCCGCGTGCA CTGGATTGCG 
GAGGAGCCTC CCGCAGCACT GGTCCGGGAC AAGATGATGG AGTGCCACTT CCGATTCCGC 
CACCAGATGG CACTAGTGCC CTGTGTGCTG ACCCTCAATC AAGATGGCAC CGTGTGGGTG 
ACAGCTGTGC AGGCTGTGCG TGCCCTTGCC ACAGGACAGT TTGCTGTGTT CTACAAGGGG 
GACGAGTGCC TGGGCAGCGG GAAGATCCTG CGGCTGGGGC CGTCTGCCTA CACGCTCCAG 
AAGGGCCAGC GCAGAGCTGG GATGGCCACT GAGAGCCCCA GTGACAGCCC AGAAGATGGT 
CCAGGCCTGA GTCCCTTGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMU ( NM_001282784.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269713.1 Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu07908
Clone ID Related Accession (Same CDS sequence)	NM_001282784.1 , NM_001282784.2
Accession Version	NM_001282784.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	mitochondrial tRNA-specific 2-thiouridylase 1 isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN997542.1, AY062123.2, DQ310000.1, AL031588.1 and BC066593.1. On Sep 19, 2013 this sequence version replaced XM_005261682.1. Transcript Variant: This variant (6) contains multiple differences in the coding region, including initiation of translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (f) is shorter and has a distinct C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ310000.1, BC080631.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCATTGC CACAGGTCAC TATGCAAGAA CTTCCCTGGA AGATGAAGAA GTCTTTGAGC 
AGAAGCACGT TAAGAAGCCC GAAGGGCTTT TCAGAAATCG GTTTGAAGTT AGAAATGGTT 
TCCCAGGATG CCCTGAGGAG AACCATCTTC CCTCTGGGGG GATTAACGAA AGAGTTTGTA 
AAGAAAATCG CTGCTGAGAA TAGACTTCAT CATGTGCTTC AGAAGAAAGA GAGCATGGGC 
ATGTGTTTCA TCGGGAAGAG GAATTTTGAA CATTTCCTTC TTCAGTATCT GCAGCCTCGA 
CCTGGTCACT TTATTTCCAT AGAAGACAAT AAGGTTCTGG GAACACATAA AGGTTGGTTC 
CTGTATACCT TGGGCCAGAG AGCAAACATA GGTGGCCTGA GAGAGCCCTG GTACGTGGTG 
GAGAAGGACA GCGTCAAGGG TGACGTGTTT GTGGCCCCCC GGACAGACCA CCCAGCCCTG 
TACAGGGACC TGCTGAGGAC CAGCCGCGTG CACTGGATTG CGGAGGAGCC TCCCGCAGCA 
CTGGTCCGGG ACAAGATGAT GGAGTGCCAC TTCCGATTCC GCCACCAGAT GGCACTAGTT 
TGCTGTGTTC TACAAGGGGG ACGAGTGCCT GGGCAGCGGG AAGATCCTGC GGCTGGGGCC 
GTCTGCCTAC ACGCTCCAGA AGGGCCAGCG CAGAGCTGGG ATGGCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269713.1
CDS	619..1329
Translation	MPLPQVTMQELPWKMKKSLSRSTLRSPKGFSEIGLKLEMVSQDALRRTIFPLGGLTKEFVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHFISIEDNKVLGTHKGWFLYTLGQRANIGGLREPWYVVEKDSVKGDVFVAPRTDHPALYRDLLRTSRVHWIAEEPPAALVRDKMMECHFRFRHQMALVCCVLQGGRVPGQREDPAAGAVCLHAPEGPAQSWDGH

Target ORF information:

RefSeq Version	NM_001282784.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282784.1

ORF Insert Sequence:

ATGCCATTGC CACAGGTCAC TATGCAAGAA CTTCCCTGGA AGATGAAGAA GTCTTTGAGC 
AGAAGCACGT TAAGAAGCCC GAAGGGCTTT TCAGAAATCG GTTTGAAGTT AGAAATGGTT 
TCCCAGGATG CCCTGAGGAG AACCATCTTC CCTCTGGGGG GATTAACGAA AGAGTTTGTA 
AAGAAAATCG CTGCTGAGAA TAGACTTCAT CATGTGCTTC AGAAGAAAGA GAGCATGGGC 
ATGTGTTTCA TCGGGAAGAG GAATTTTGAA CATTTCCTTC TTCAGTATCT GCAGCCTCGA 
CCTGGTCACT TTATTTCCAT AGAAGACAAT AAGGTTCTGG GAACACATAA AGGTTGGTTC 
CTGTATACCT TGGGCCAGAG AGCAAACATA GGTGGCCTGA GAGAGCCCTG GTACGTGGTG 
GAGAAGGACA GCGTCAAGGG TGACGTGTTT GTGGCCCCCC GGACAGACCA CCCAGCCCTG 
TACAGGGACC TGCTGAGGAC CAGCCGCGTG CACTGGATTG CGGAGGAGCC TCCCGCAGCA 
CTGGTCCGGG ACAAGATGAT GGAGTGCCAC TTCCGATTCC GCCACCAGAT GGCACTAGTT 
TGCTGTGTTC TACAAGGGGG ACGAGTGCCT GGGCAGCGGG AAGATCCTGC GGCTGGGGCC 
GTCTGCCTAC ACGCTCCAGA AGGGCCAGCG CAGAGCTGGG ATGGCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMU ( NM_001282784.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269713.1 Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu07908
Clone ID Related Accession (Same CDS sequence)	NM_001282784.1 , NM_001282784.2
Accession Version	NM_001282784.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-20
Organism	Homo sapiens(human)
Product	mitochondrial tRNA-specific 2-thiouridylase 1 isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY062123.2, DQ310000.1, AL031588.1 and BC066593.1. On May 31, 2019 this sequence version replaced NM_001282784.1. Transcript Variant: This variant (6) contains multiple differences in the coding region, including initiation of translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (f) is shorter and has a distinct C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ310000.1, BC080631.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCATTGC CACAGGTCAC TATGCAAGAA CTTCCCTGGA AGATGAAGAA GTCTTTGAGC 
AGAAGCACGT TAAGAAGCCC GAAGGGCTTT TCAGAAATCG GTTTGAAGTT AGAAATGGTT 
TCCCAGGATG CCCTGAGGAG AACCATCTTC CCTCTGGGGG GATTAACGAA AGAGTTTGTA 
AAGAAAATCG CTGCTGAGAA TAGACTTCAT CATGTGCTTC AGAAGAAAGA GAGCATGGGC 
ATGTGTTTCA TCGGGAAGAG GAATTTTGAA CATTTCCTTC TTCAGTATCT GCAGCCTCGA 
CCTGGTCACT TTATTTCCAT AGAAGACAAT AAGGTTCTGG GAACACATAA AGGTTGGTTC 
CTGTATACCT TGGGCCAGAG AGCAAACATA GGTGGCCTGA GAGAGCCCTG GTACGTGGTG 
GAGAAGGACA GCGTCAAGGG TGACGTGTTT GTGGCCCCCC GGACAGACCA CCCAGCCCTG 
TACAGGGACC TGCTGAGGAC CAGCCGCGTG CACTGGATTG CGGAGGAGCC TCCCGCAGCA 
CTGGTCCGGG ACAAGATGAT GGAGTGCCAC TTCCGATTCC GCCACCAGAT GGCACTAGTT 
TGCTGTGTTC TACAAGGGGG ACGAGTGCCT GGGCAGCGGG AAGATCCTGC GGCTGGGGCC 
GTCTGCCTAC ACGCTCCAGA AGGGCCAGCG CAGAGCTGGG ATGGCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269713.1
CDS	306..1016
Translation	MPLPQVTMQELPWKMKKSLSRSTLRSPKGFSEIGLKLEMVSQDALRRTIFPLGGLTKEFVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHFISIEDNKVLGTHKGWFLYTLGQRANIGGLREPWYVVEKDSVKGDVFVAPRTDHPALYRDLLRTSRVHWIAEEPPAALVRDKMMECHFRFRHQMALVCCVLQGGRVPGQREDPAAGAVCLHAPEGPAQSWDGH

Target ORF information:

RefSeq Version	NM_001282784.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282784.2

ORF Insert Sequence:

ATGCCATTGC CACAGGTCAC TATGCAAGAA CTTCCCTGGA AGATGAAGAA GTCTTTGAGC 
AGAAGCACGT TAAGAAGCCC GAAGGGCTTT TCAGAAATCG GTTTGAAGTT AGAAATGGTT 
TCCCAGGATG CCCTGAGGAG AACCATCTTC CCTCTGGGGG GATTAACGAA AGAGTTTGTA 
AAGAAAATCG CTGCTGAGAA TAGACTTCAT CATGTGCTTC AGAAGAAAGA GAGCATGGGC 
ATGTGTTTCA TCGGGAAGAG GAATTTTGAA CATTTCCTTC TTCAGTATCT GCAGCCTCGA 
CCTGGTCACT TTATTTCCAT AGAAGACAAT AAGGTTCTGG GAACACATAA AGGTTGGTTC 
CTGTATACCT TGGGCCAGAG AGCAAACATA GGTGGCCTGA GAGAGCCCTG GTACGTGGTG 
GAGAAGGACA GCGTCAAGGG TGACGTGTTT GTGGCCCCCC GGACAGACCA CCCAGCCCTG 
TACAGGGACC TGCTGAGGAC CAGCCGCGTG CACTGGATTG CGGAGGAGCC TCCCGCAGCA 
CTGGTCCGGG ACAAGATGAT GGAGTGCCAC TTCCGATTCC GCCACCAGAT GGCACTAGTT 
TGCTGTGTTC TACAAGGGGG ACGAGTGCCT GGGCAGCGGG AAGATCCTGC GGCTGGGGCC 
GTCTGCCTAC ACGCTCCAGA AGGGCCAGCG CAGAGCTGGG ATGGCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMU ( NM_001282785.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269714.1 Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu10076
Clone ID Related Accession (Same CDS sequence)	NM_001282785.1 , NM_001282785.2
Accession Version	NM_001282785.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	mitochondrial tRNA-specific 2-thiouridylase 1 isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN997542.1, AY062123.2, CR456445.1 and BC066593.1. On Sep 19, 2013 this sequence version replaced XM_005261676.1. Transcript Variant: This variant (7) lacks an alternate exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (g) is shorter and has a distinct C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.144583.1, CR456445.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGGCCT TGCGGCACGT CGTGTGCGCC CTGTCCGGCG GCGTGGACAG CGCCGTGGCC 
GCGCTGCTGC TGAGGCGGAG AGGTTACCAG GTGACAGGGG TGTTTATGAA GAACTGGGAC 
TCACTGGATG AACATGGGGT CTGTACTGCC GACAAAGACT GTGAAGATGC TTACAGAGTT 
TGCCAGATCT TAGACATCCC TTTCCATCAA GTGTCCTACG TAAAGGAGTA TTGGAATGAT 
GTGTTCAGTG ACTTTTTGAA TGAGTATGAA AAAGGAAGGA CTCCCAATCC TGACATAGTT 
TGCAACAAGC ACATCAAATT TAGTTGCTTT TTTCATTATG CTGTGGATAA TCTTGGGGCA 
GATGCCATTG CCACAGGTCA CTATGCAAGA ACTTCCCTGG AAGATGAAGA AGTCTTTGAG 
CAGAAGCACG TTAAGAAGCC CGAAGGGCTT TTCAGAAATC GGTTTGAAGT TAGAAATGCG 
GTAAAACTCC TCCAGGCAGC TGACAGCTTT AAAGACCAGA CCTTCTTTCT CAGCCAGGTT 
TCCCAGGATG CCCTGAGGAG AACCATCTTC CCTCTGGGGG GATTAACGAA AGAGTTTGTA 
AAGAAAATCG CTGCTGAGAA TAGACTTCAT CATGTGCTTC AGAAGAAAGA GAGCATGGGC 
ATGTGTTTCA TCGGGAAGAG GAATTTTGAA CATTTCCTTC TTCAGTATCT GCAGCCTCGA 
CCTGGTCACT TTATTTCCAT AGAAGACAAT AAGGTTCTGG GAACACATAA AGGTTGGTTC 
CTGTATACCT TGGGCCAGAG AGCAAACATA GGTGGCCTGA GAGAGCCCTG GTACGTGGTG 
GAGAAGGACA GCGTCAAGGG TGACGTGTTT GTGGCCCCCC GGACAGACCA CCCAGCCCTG 
TACAGGGACC TGCTGAGGAC CAGCCGCGTG CACTGGATTG CGGAGGAGCC TCCCGCAGCA 
CTGGTCCGGG ACAAGATGAT GGAGTGCCAC TTCCGATTCC GCCACCAGAT GGCACTAGTT 
TGCTGTGTTC TACAAGGGGG ACGAGTGCCT GGGCAGCGGG AAGATCCTGC GGCTGGGGCC 
GTCTGCCTAC ACGCTCCAGA AGGGCCAGCG CAGAGCTGGG ATGGCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269714.1
CDS	365..1495
Translation	MQALRHVVCALSGGVDSAVAALLLRRRGYQVTGVFMKNWDSLDEHGVCTADKDCEDAYRVCQILDIPFHQVSYVKEYWNDVFSDFLNEYEKGRTPNPDIVCNKHIKFSCFFHYAVDNLGADAIATGHYARTSLEDEEVFEQKHVKKPEGLFRNRFEVRNAVKLLQAADSFKDQTFFLSQVSQDALRRTIFPLGGLTKEFVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHFISIEDNKVLGTHKGWFLYTLGQRANIGGLREPWYVVEKDSVKGDVFVAPRTDHPALYRDLLRTSRVHWIAEEPPAALVRDKMMECHFRFRHQMALVCCVLQGGRVPGQREDPAAGAVCLHAPEGPAQSWDGH

Target ORF information:

RefSeq Version	NM_001282785.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282785.1

ORF Insert Sequence:

ATGCAGGCCT TGCGGCACGT CGTGTGCGCC CTGTCCGGCG GCGTGGACAG CGCCGTGGCC 
GCGCTGCTGC TGAGGCGGAG AGGTTACCAG GTGACAGGGG TGTTTATGAA GAACTGGGAC 
TCACTGGATG AACATGGGGT CTGTACTGCC GACAAAGACT GTGAAGATGC TTACAGAGTT 
TGCCAGATCT TAGACATCCC TTTCCATCAA GTGTCCTACG TAAAGGAGTA TTGGAATGAT 
GTGTTCAGTG ACTTTTTGAA TGAGTATGAA AAAGGAAGGA CTCCCAATCC TGACATAGTT 
TGCAACAAGC ACATCAAATT TAGTTGCTTT TTTCATTATG CTGTGGATAA TCTTGGGGCA 
GATGCCATTG CCACAGGTCA CTATGCAAGA ACTTCCCTGG AAGATGAAGA AGTCTTTGAG 
CAGAAGCACG TTAAGAAGCC CGAAGGGCTT TTCAGAAATC GGTTTGAAGT TAGAAATGCG 
GTAAAACTCC TCCAGGCAGC TGACAGCTTT AAAGACCAGA CCTTCTTTCT CAGCCAGGTT 
TCCCAGGATG CCCTGAGGAG AACCATCTTC CCTCTGGGGG GATTAACGAA AGAGTTTGTA 
AAGAAAATCG CTGCTGAGAA TAGACTTCAT CATGTGCTTC AGAAGAAAGA GAGCATGGGC 
ATGTGTTTCA TCGGGAAGAG GAATTTTGAA CATTTCCTTC TTCAGTATCT GCAGCCTCGA 
CCTGGTCACT TTATTTCCAT AGAAGACAAT AAGGTTCTGG GAACACATAA AGGTTGGTTC 
CTGTATACCT TGGGCCAGAG AGCAAACATA GGTGGCCTGA GAGAGCCCTG GTACGTGGTG 
GAGAAGGACA GCGTCAAGGG TGACGTGTTT GTGGCCCCCC GGACAGACCA CCCAGCCCTG 
TACAGGGACC TGCTGAGGAC CAGCCGCGTG CACTGGATTG CGGAGGAGCC TCCCGCAGCA 
CTGGTCCGGG ACAAGATGAT GGAGTGCCAC TTCCGATTCC GCCACCAGAT GGCACTAGTT 
TGCTGTGTTC TACAAGGGGG ACGAGTGCCT GGGCAGCGGG AAGATCCTGC GGCTGGGGCC 
GTCTGCCTAC ACGCTCCAGA AGGGCCAGCG CAGAGCTGGG ATGGCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMU ( NM_001282785.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269714.1 Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu10076
Clone ID Related Accession (Same CDS sequence)	NM_001282785.1 , NM_001282785.2
Accession Version	NM_001282785.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	mitochondrial tRNA-specific 2-thiouridylase 1 isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY062123.2, CR456445.1 and BC066593.1. On May 31, 2019 this sequence version replaced NM_001282785.1. Transcript Variant: This variant (7) lacks an alternate exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (g) is shorter and has a distinct C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.296043.1, SRR1803613.144583.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGGCCT TGCGGCACGT CGTGTGCGCC CTGTCCGGCG GCGTGGACAG CGCCGTGGCC 
GCGCTGCTGC TGAGGCGGAG AGGTTACCAG GTGACAGGGG TGTTTATGAA GAACTGGGAC 
TCACTGGATG AACATGGGGT CTGTACTGCC GACAAAGACT GTGAAGATGC TTACAGAGTT 
TGCCAGATCT TAGACATCCC TTTCCATCAA GTGTCCTACG TAAAGGAGTA TTGGAATGAT 
GTGTTCAGTG ACTTTTTGAA TGAGTATGAA AAAGGAAGGA CTCCCAATCC TGACATAGTT 
TGCAACAAGC ACATCAAATT TAGTTGCTTT TTTCATTATG CTGTGGATAA TCTTGGGGCA 
GATGCCATTG CCACAGGTCA CTATGCAAGA ACTTCCCTGG AAGATGAAGA AGTCTTTGAG 
CAGAAGCACG TTAAGAAGCC CGAAGGGCTT TTCAGAAATC GGTTTGAAGT TAGAAATGCG 
GTAAAACTCC TCCAGGCAGC TGACAGCTTT AAAGACCAGA CCTTCTTTCT CAGCCAGGTT 
TCCCAGGATG CCCTGAGGAG AACCATCTTC CCTCTGGGGG GATTAACGAA AGAGTTTGTA 
AAGAAAATCG CTGCTGAGAA TAGACTTCAT CATGTGCTTC AGAAGAAAGA GAGCATGGGC 
ATGTGTTTCA TCGGGAAGAG GAATTTTGAA CATTTCCTTC TTCAGTATCT GCAGCCTCGA 
CCTGGTCACT TTATTTCCAT AGAAGACAAT AAGGTTCTGG GAACACATAA AGGTTGGTTC 
CTGTATACCT TGGGCCAGAG AGCAAACATA GGTGGCCTGA GAGAGCCCTG GTACGTGGTG 
GAGAAGGACA GCGTCAAGGG TGACGTGTTT GTGGCCCCCC GGACAGACCA CCCAGCCCTG 
TACAGGGACC TGCTGAGGAC CAGCCGCGTG CACTGGATTG CGGAGGAGCC TCCCGCAGCA 
CTGGTCCGGG ACAAGATGAT GGAGTGCCAC TTCCGATTCC GCCACCAGAT GGCACTAGTT 
TGCTGTGTTC TACAAGGGGG ACGAGTGCCT GGGCAGCGGG AAGATCCTGC GGCTGGGGCC 
GTCTGCCTAC ACGCTCCAGA AGGGCCAGCG CAGAGCTGGG ATGGCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269714.1
CDS	52..1182
Translation	MQALRHVVCALSGGVDSAVAALLLRRRGYQVTGVFMKNWDSLDEHGVCTADKDCEDAYRVCQILDIPFHQVSYVKEYWNDVFSDFLNEYEKGRTPNPDIVCNKHIKFSCFFHYAVDNLGADAIATGHYARTSLEDEEVFEQKHVKKPEGLFRNRFEVRNAVKLLQAADSFKDQTFFLSQVSQDALRRTIFPLGGLTKEFVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHFISIEDNKVLGTHKGWFLYTLGQRANIGGLREPWYVVEKDSVKGDVFVAPRTDHPALYRDLLRTSRVHWIAEEPPAALVRDKMMECHFRFRHQMALVCCVLQGGRVPGQREDPAAGAVCLHAPEGPAQSWDGH

Target ORF information:

RefSeq Version	NM_001282785.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282785.2

ORF Insert Sequence:

ATGCAGGCCT TGCGGCACGT CGTGTGCGCC CTGTCCGGCG GCGTGGACAG CGCCGTGGCC 
GCGCTGCTGC TGAGGCGGAG AGGTTACCAG GTGACAGGGG TGTTTATGAA GAACTGGGAC 
TCACTGGATG AACATGGGGT CTGTACTGCC GACAAAGACT GTGAAGATGC TTACAGAGTT 
TGCCAGATCT TAGACATCCC TTTCCATCAA GTGTCCTACG TAAAGGAGTA TTGGAATGAT 
GTGTTCAGTG ACTTTTTGAA TGAGTATGAA AAAGGAAGGA CTCCCAATCC TGACATAGTT 
TGCAACAAGC ACATCAAATT TAGTTGCTTT TTTCATTATG CTGTGGATAA TCTTGGGGCA 
GATGCCATTG CCACAGGTCA CTATGCAAGA ACTTCCCTGG AAGATGAAGA AGTCTTTGAG 
CAGAAGCACG TTAAGAAGCC CGAAGGGCTT TTCAGAAATC GGTTTGAAGT TAGAAATGCG 
GTAAAACTCC TCCAGGCAGC TGACAGCTTT AAAGACCAGA CCTTCTTTCT CAGCCAGGTT 
TCCCAGGATG CCCTGAGGAG AACCATCTTC CCTCTGGGGG GATTAACGAA AGAGTTTGTA 
AAGAAAATCG CTGCTGAGAA TAGACTTCAT CATGTGCTTC AGAAGAAAGA GAGCATGGGC 
ATGTGTTTCA TCGGGAAGAG GAATTTTGAA CATTTCCTTC TTCAGTATCT GCAGCCTCGA 
CCTGGTCACT TTATTTCCAT AGAAGACAAT AAGGTTCTGG GAACACATAA AGGTTGGTTC 
CTGTATACCT TGGGCCAGAG AGCAAACATA GGTGGCCTGA GAGAGCCCTG GTACGTGGTG 
GAGAAGGACA GCGTCAAGGG TGACGTGTTT GTGGCCCCCC GGACAGACCA CCCAGCCCTG 
TACAGGGACC TGCTGAGGAC CAGCCGCGTG CACTGGATTG CGGAGGAGCC TCCCGCAGCA 
CTGGTCCGGG ACAAGATGAT GGAGTGCCAC TTCCGATTCC GCCACCAGAT GGCACTAGTT 
TGCTGTGTTC TACAAGGGGG ACGAGTGCCT GGGCAGCGGG AAGATCCTGC GGCTGGGGCC 
GTCTGCCTAC ACGCTCCAGA AGGGCCAGCG CAGAGCTGGG ATGGCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMU ( NM_018006.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060476.2 Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 1, mRNA.

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CloneID	OHu19683
Clone ID Related Accession (Same CDS sequence)	NM_018006.5 , NM_018006.4
Accession Version	NM_018006.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	mitochondrial tRNA-specific 2-thiouridylase 1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY062123.2 and BC066593.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_018006.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC066593.1, AY062123.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000645190.1/ ENSP00000496496.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGGCCT TGCGGCACGT CGTGTGCGCC CTGTCCGGCG GCGTGGACAG CGCCGTGGCC 
GCGCTGCTGC TGAGGCGGAG AGGTTACCAG GTGACAGGGG TGTTTATGAA GAACTGGGAC 
TCACTGGATG AACATGGGGT CTGTACTGCC GACAAAGACT GTGAAGATGC TTACAGAGTT 
TGCCAGATCT TAGACATCCC TTTCCATCAA GTGTCCTACG TAAAGGAGTA TTGGAATGAT 
GTGTTCAGTG ACTTTTTGAA TGAGTATGAA AAAGGAAGGA CTCCCAATCC TGACATAGTT 
TGCAACAAGC ACATCAAATT TAGTTGCTTT TTTCATTATG CTGTGGATAA TCTTGGGGCA 
GATGCCATTG CCACAGGTCA CTATGCAAGA ACTTCCCTGG AAGATGAAGA AGTCTTTGAG 
CAGAAGCACG TTAAGAAGCC CGAAGGGCTT TTCAGAAATC GGTTTGAAGT TAGAAATGCG 
GTAAAACTCC TCCAGGCAGC TGACAGCTTT AAAGACCAGA CCTTCTTTCT CAGCCAGGTT 
TCCCAGGATG CCCTGAGGAG AACCATCTTC CCTCTGGGGG GATTAACGAA AGAGTTTGTA 
AAGAAAATCG CTGCTGAGAA TAGACTTCAT CATGTGCTTC AGAAGAAAGA GAGCATGGGC 
ATGTGTTTCA TCGGGAAGAG GAATTTTGAA CATTTCCTTC TTCAGTATCT GCAGCCTCGA 
CCTGGTCACT TTATTTCCAT AGAAGACAAT AAGGTTCTGG GAACACATAA AGGTTGGTTC 
CTGTATACCT TGGGCCAGAG AGCAAACATA GGTGGCCTGA GAGAGCCCTG GTACGTGGTG 
GAGAAGGACA GCGTCAAGGG TGACGTGTTT GTGGCCCCCC GGACAGACCA CCCAGCCCTG 
TACAGGGACC TGCTGAGGAC CAGCCGCGTG CACTGGATTG CGGAGGAGCC TCCCGCAGCA 
CTGGTCCGGG ACAAGATGAT GGAGTGCCAC TTCCGATTCC GCCACCAGAT GGCACTAGTG 
CCCTGTGTGC TGACCCTCAA TCAAGATGGC ACCGTGTGGG TGACAGCTGT GCAGGCTGTG 
CGTGCCCTTG CCACAGGACA GTTTGCTGTG TTCTACAAGG GGGACGAGTG CCTGGGCAGC 
GGGAAGATCC TGCGGCTGGG GCCGTCTGCC TACACGCTCC AGAAGGGCCA GCGCAGAGCT 
GGGATGGCCA CTGAGAGCCC CAGTGACAGC CCAGAAGATG GTCCAGGCCT GAGTCCCTTG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060476.2
CDS	52..1317
Translation	MQALRHVVCALSGGVDSAVAALLLRRRGYQVTGVFMKNWDSLDEHGVCTADKDCEDAYRVCQILDIPFHQVSYVKEYWNDVFSDFLNEYEKGRTPNPDIVCNKHIKFSCFFHYAVDNLGADAIATGHYARTSLEDEEVFEQKHVKKPEGLFRNRFEVRNAVKLLQAADSFKDQTFFLSQVSQDALRRTIFPLGGLTKEFVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHFISIEDNKVLGTHKGWFLYTLGQRANIGGLREPWYVVEKDSVKGDVFVAPRTDHPALYRDLLRTSRVHWIAEEPPAALVRDKMMECHFRFRHQMALVPCVLTLNQDGTVWVTAVQAVRALATGQFAVFYKGDECLGSGKILRLGPSAYTLQKGQRRAGMATESPSDSPEDGPGLSPLL

Target ORF information:

RefSeq Version	NM_018006.5
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018006.5

ORF Insert Sequence:

ATGCAGGCCT TGCGGCACGT CGTGTGCGCC CTGTCCGGCG GCGTGGACAG CGCCGTGGCC 
GCGCTGCTGC TGAGGCGGAG AGGTTACCAG GTGACAGGGG TGTTTATGAA GAACTGGGAC 
TCACTGGATG AACATGGGGT CTGTACTGCC GACAAAGACT GTGAAGATGC TTACAGAGTT 
TGCCAGATCT TAGACATCCC TTTCCATCAA GTGTCCTACG TAAAGGAGTA TTGGAATGAT 
GTGTTCAGTG ACTTTTTGAA TGAGTATGAA AAAGGAAGGA CTCCCAATCC TGACATAGTT 
TGCAACAAGC ACATCAAATT TAGTTGCTTT TTTCATTATG CTGTGGATAA TCTTGGGGCA 
GATGCCATTG CCACAGGTCA CTATGCAAGA ACTTCCCTGG AAGATGAAGA AGTCTTTGAG 
CAGAAGCACG TTAAGAAGCC CGAAGGGCTT TTCAGAAATC GGTTTGAAGT TAGAAATGCG 
GTAAAACTCC TCCAGGCAGC TGACAGCTTT AAAGACCAGA CCTTCTTTCT CAGCCAGGTT 
TCCCAGGATG CCCTGAGGAG AACCATCTTC CCTCTGGGGG GATTAACGAA AGAGTTTGTA 
AAGAAAATCG CTGCTGAGAA TAGACTTCAT CATGTGCTTC AGAAGAAAGA GAGCATGGGC 
ATGTGTTTCA TCGGGAAGAG GAATTTTGAA CATTTCCTTC TTCAGTATCT GCAGCCTCGA 
CCTGGTCACT TTATTTCCAT AGAAGACAAT AAGGTTCTGG GAACACATAA AGGTTGGTTC 
CTGTATACCT TGGGCCAGAG AGCAAACATA GGTGGCCTGA GAGAGCCCTG GTACGTGGTG 
GAGAAGGACA GCGTCAAGGG TGACGTGTTT GTGGCCCCCC GGACAGACCA CCCAGCCCTG 
TACAGGGACC TGCTGAGGAC CAGCCGCGTG CACTGGATTG CGGAGGAGCC TCCCGCAGCA 
CTGGTCCGGG ACAAGATGAT GGAGTGCCAC TTCCGATTCC GCCACCAGAT GGCACTAGTG 
CCCTGTGTGC TGACCCTCAA TCAAGATGGC ACCGTGTGGG TGACAGCTGT GCAGGCTGTG 
CGTGCCCTTG CCACAGGACA GTTTGCTGTG TTCTACAAGG GGGACGAGTG CCTGGGCAGC 
GGGAAGATCC TGCGGCTGGG GCCGTCTGCC TACACGCTCC AGAAGGGCCA GCGCAGAGCT 
GGGATGGCCA CTGAGAGCCC CAGTGACAGC CCAGAAGATG GTCCAGGCCT GAGTCCCTTG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMU ( NM_018006.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060476.2 Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19683
Clone ID Related Accession (Same CDS sequence)	NM_018006.5 , NM_018006.4
Accession Version	NM_018006.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-30
Organism	Homo sapiens(human)
Product	mitochondrial tRNA-specific 2-thiouridylase 1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN997542.1, AY062123.2 and BC066593.1. This sequence is a reference standard in the RefSeqGene project. On Dec 6, 2005 this sequence version replaced NM_018006.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC066593.1, AY062123.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGGCCT TGCGGCACGT CGTGTGCGCC CTGTCCGGCG GCGTGGACAG CGCCGTGGCC 
GCGCTGCTGC TGAGGCGGAG AGGTTACCAG GTGACAGGGG TGTTTATGAA GAACTGGGAC 
TCACTGGATG AACATGGGGT CTGTACTGCC GACAAAGACT GTGAAGATGC TTACAGAGTT 
TGCCAGATCT TAGACATCCC TTTCCATCAA GTGTCCTACG TAAAGGAGTA TTGGAATGAT 
GTGTTCAGTG ACTTTTTGAA TGAGTATGAA AAAGGAAGGA CTCCCAATCC TGACATAGTT 
TGCAACAAGC ACATCAAATT TAGTTGCTTT TTTCATTATG CTGTGGATAA TCTTGGGGCA 
GATGCCATTG CCACAGGTCA CTATGCAAGA ACTTCCCTGG AAGATGAAGA AGTCTTTGAG 
CAGAAGCACG TTAAGAAGCC CGAAGGGCTT TTCAGAAATC GGTTTGAAGT TAGAAATGCG 
GTAAAACTCC TCCAGGCAGC TGACAGCTTT AAAGACCAGA CCTTCTTTCT CAGCCAGGTT 
TCCCAGGATG CCCTGAGGAG AACCATCTTC CCTCTGGGGG GATTAACGAA AGAGTTTGTA 
AAGAAAATCG CTGCTGAGAA TAGACTTCAT CATGTGCTTC AGAAGAAAGA GAGCATGGGC 
ATGTGTTTCA TCGGGAAGAG GAATTTTGAA CATTTCCTTC TTCAGTATCT GCAGCCTCGA 
CCTGGTCACT TTATTTCCAT AGAAGACAAT AAGGTTCTGG GAACACATAA AGGTTGGTTC 
CTGTATACCT TGGGCCAGAG AGCAAACATA GGTGGCCTGA GAGAGCCCTG GTACGTGGTG 
GAGAAGGACA GCGTCAAGGG TGACGTGTTT GTGGCCCCCC GGACAGACCA CCCAGCCCTG 
TACAGGGACC TGCTGAGGAC CAGCCGCGTG CACTGGATTG CGGAGGAGCC TCCCGCAGCA 
CTGGTCCGGG ACAAGATGAT GGAGTGCCAC TTCCGATTCC GCCACCAGAT GGCACTAGTG 
CCCTGTGTGC TGACCCTCAA TCAAGATGGC ACCGTGTGGG TGACAGCTGT GCAGGCTGTG 
CGTGCCCTTG CCACAGGACA GTTTGCTGTG TTCTACAAGG GGGACGAGTG CCTGGGCAGC 
GGGAAGATCC TGCGGCTGGG GCCGTCTGCC TACACGCTCC AGAAGGGCCA GCGCAGAGCT 
GGGATGGCCA CTGAGAGCCC CAGTGACAGC CCAGAAGATG GTCCAGGCCT GAGTCCCTTG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060476.2
CDS	365..1630
Translation	MQALRHVVCALSGGVDSAVAALLLRRRGYQVTGVFMKNWDSLDEHGVCTADKDCEDAYRVCQILDIPFHQVSYVKEYWNDVFSDFLNEYEKGRTPNPDIVCNKHIKFSCFFHYAVDNLGADAIATGHYARTSLEDEEVFEQKHVKKPEGLFRNRFEVRNAVKLLQAADSFKDQTFFLSQVSQDALRRTIFPLGGLTKEFVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHFISIEDNKVLGTHKGWFLYTLGQRANIGGLREPWYVVEKDSVKGDVFVAPRTDHPALYRDLLRTSRVHWIAEEPPAALVRDKMMECHFRFRHQMALVPCVLTLNQDGTVWVTAVQAVRALATGQFAVFYKGDECLGSGKILRLGPSAYTLQKGQRRAGMATESPSDSPEDGPGLSPLL

Target ORF information:

RefSeq Version	NM_018006.4
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018006.4

ORF Insert Sequence:

ATGCAGGCCT TGCGGCACGT CGTGTGCGCC CTGTCCGGCG GCGTGGACAG CGCCGTGGCC 
GCGCTGCTGC TGAGGCGGAG AGGTTACCAG GTGACAGGGG TGTTTATGAA GAACTGGGAC 
TCACTGGATG AACATGGGGT CTGTACTGCC GACAAAGACT GTGAAGATGC TTACAGAGTT 
TGCCAGATCT TAGACATCCC TTTCCATCAA GTGTCCTACG TAAAGGAGTA TTGGAATGAT 
GTGTTCAGTG ACTTTTTGAA TGAGTATGAA AAAGGAAGGA CTCCCAATCC TGACATAGTT 
TGCAACAAGC ACATCAAATT TAGTTGCTTT TTTCATTATG CTGTGGATAA TCTTGGGGCA 
GATGCCATTG CCACAGGTCA CTATGCAAGA ACTTCCCTGG AAGATGAAGA AGTCTTTGAG 
CAGAAGCACG TTAAGAAGCC CGAAGGGCTT TTCAGAAATC GGTTTGAAGT TAGAAATGCG 
GTAAAACTCC TCCAGGCAGC TGACAGCTTT AAAGACCAGA CCTTCTTTCT CAGCCAGGTT 
TCCCAGGATG CCCTGAGGAG AACCATCTTC CCTCTGGGGG GATTAACGAA AGAGTTTGTA 
AAGAAAATCG CTGCTGAGAA TAGACTTCAT CATGTGCTTC AGAAGAAAGA GAGCATGGGC 
ATGTGTTTCA TCGGGAAGAG GAATTTTGAA CATTTCCTTC TTCAGTATCT GCAGCCTCGA 
CCTGGTCACT TTATTTCCAT AGAAGACAAT AAGGTTCTGG GAACACATAA AGGTTGGTTC 
CTGTATACCT TGGGCCAGAG AGCAAACATA GGTGGCCTGA GAGAGCCCTG GTACGTGGTG 
GAGAAGGACA GCGTCAAGGG TGACGTGTTT GTGGCCCCCC GGACAGACCA CCCAGCCCTG 
TACAGGGACC TGCTGAGGAC CAGCCGCGTG CACTGGATTG CGGAGGAGCC TCCCGCAGCA 
CTGGTCCGGG ACAAGATGAT GGAGTGCCAC TTCCGATTCC GCCACCAGAT GGCACTAGTG 
CCCTGTGTGC TGACCCTCAA TCAAGATGGC ACCGTGTGGG TGACAGCTGT GCAGGCTGTG 
CGTGCCCTTG CCACAGGACA GTTTGCTGTG TTCTACAAGG GGGACGAGTG CCTGGGCAGC 
GGGAAGATCC TGCGGCTGGG GCCGTCTGCC TACACGCTCC AGAAGGGCCA GCGCAGAGCT 
GGGATGGCCA CTGAGAGCCC CAGTGACAGC CCAGAAGATG GTCCAGGCCT GAGTCCCTTG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMU ( XM_011530274.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011528576.1 Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu00544
Clone ID Related Accession (Same CDS sequence)	XM_011530274.2 , NM_001282782.2 , NM_001282782.1
Accession Version	XM_011530274.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitochondrial tRNA-specific 2-thiouridylase 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530274.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGTT CAGGGGCAGA TGCCATTGCC ACAGGTCACT ATGCAAGAAC TTCCCTGGAA 
GATGAAGAAG TCTTTGAGCA GAAGCACGTT AAGAAGCCCG AAGGGCTTTT CAGAAATCGG 
TTTGAAGTTA GAAATGCGGT AAAACTCCTC CAGGCAGCTG ACAGCTTTAA AGACCAGACC 
TTCTTTCTCA GCCAGGTTTC CCAGGATGCC CTGAGGAGAA CCATCTTCCC TCTGGGGGGA 
TTAACGAAAG AGTTTGTAAA GAAAATCGCT GCTGAGAATA GACTTCATCA TGTGCTTCAG 
AAGAAAGAGA GCATGGGCAT GTGTTTCATC GGGAAGAGGA ATTTTGAACA TTTCCTTCTT 
CAGTATCTGC AGCCTCGACC TGGTCACTTT ATTTCCATAG AAGACAATAA GGTTCTGGGA 
ACACATAAAG GTTGGTTCCT GTATACCTTG GGCCAGAGAG CAAACATAGG TGGCCTGAGA 
GAGCCCTGGT ACGTGGTGGA GAAGGACAGC GTCAAGGGTG ACGTGTTTGT GGCCCCCCGG 
ACAGACCACC CAGCCCTGTA CAGGGACCTG CTGAGGACCA GCCGCGTGCA CTGGATTGCG 
GAGGAGCCTC CCGCAGCACT GGTCCGGGAC AAGATGATGG AGTGCCACTT CCGATTCCGC 
CACCAGATGG CACTAGTGCC CTGTGTGCTG ACCCTCAATC AAGATGGCAC CGTGTGGGTG 
ACAGCTGTGC AGGCTGTGCG TGCCCTTGCC ACAGGACAGT TTGCTGTGTT CTACAAGGGG 
GACGAGTGCC TGGGCAGCGG GAAGATCCTG CGGCTGGGGC CGTCTGCCTA CACGCTCCAG 
AAGGGCCAGC GCAGAGCTGG GATGGCCACT GAGAGCCCCA GTGACAGCCC AGAAGATGGT 
CCAGGCCTGA GTCCCTTGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011528576.1
CDS	307..1230
Translation	MMCSGADAIATGHYARTSLEDEEVFEQKHVKKPEGLFRNRFEVRNAVKLLQAADSFKDQTFFLSQVSQDALRRTIFPLGGLTKEFVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHFISIEDNKVLGTHKGWFLYTLGQRANIGGLREPWYVVEKDSVKGDVFVAPRTDHPALYRDLLRTSRVHWIAEEPPAALVRDKMMECHFRFRHQMALVPCVLTLNQDGTVWVTAVQAVRALATGQFAVFYKGDECLGSGKILRLGPSAYTLQKGQRRAGMATESPSDSPEDGPGLSPLL

Target ORF information:

RefSeq Version	XM_011530274.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530274.2

ORF Insert Sequence:

ATGATGTGTT CAGGGGCAGA TGCCATTGCC ACAGGTCACT ATGCAAGAAC TTCCCTGGAA 
GATGAAGAAG TCTTTGAGCA GAAGCACGTT AAGAAGCCCG AAGGGCTTTT CAGAAATCGG 
TTTGAAGTTA GAAATGCGGT AAAACTCCTC CAGGCAGCTG ACAGCTTTAA AGACCAGACC 
TTCTTTCTCA GCCAGGTTTC CCAGGATGCC CTGAGGAGAA CCATCTTCCC TCTGGGGGGA 
TTAACGAAAG AGTTTGTAAA GAAAATCGCT GCTGAGAATA GACTTCATCA TGTGCTTCAG 
AAGAAAGAGA GCATGGGCAT GTGTTTCATC GGGAAGAGGA ATTTTGAACA TTTCCTTCTT 
CAGTATCTGC AGCCTCGACC TGGTCACTTT ATTTCCATAG AAGACAATAA GGTTCTGGGA 
ACACATAAAG GTTGGTTCCT GTATACCTTG GGCCAGAGAG CAAACATAGG TGGCCTGAGA 
GAGCCCTGGT ACGTGGTGGA GAAGGACAGC GTCAAGGGTG ACGTGTTTGT GGCCCCCCGG 
ACAGACCACC CAGCCCTGTA CAGGGACCTG CTGAGGACCA GCCGCGTGCA CTGGATTGCG 
GAGGAGCCTC CCGCAGCACT GGTCCGGGAC AAGATGATGG AGTGCCACTT CCGATTCCGC 
CACCAGATGG CACTAGTGCC CTGTGTGCTG ACCCTCAATC AAGATGGCAC CGTGTGGGTG 
ACAGCTGTGC AGGCTGTGCG TGCCCTTGCC ACAGGACAGT TTGCTGTGTT CTACAAGGGG 
GACGAGTGCC TGGGCAGCGG GAAGATCCTG CGGCTGGGGC CGTCTGCCTA CACGCTCCAG 
AAGGGCCAGC GCAGAGCTGG GATGGCCACT GAGAGCCCCA GTGACAGCCC AGAAGATGGT 
CCAGGCCTGA GTCCCTTGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMU ( XM_005261678.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005261735.1 Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu53634
Clone ID Related Accession (Same CDS sequence)	XM_005261678.1 , XM_011530275.1
Accession Version	XM_005261678.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitochondrial tRNA-specific 2-thiouridylase 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGGA TAATCTTGGG GCAGATGCCA TTGCCACAGG TCACTATGCA AGAACTTCCC 
TGGAAGATGA AGAAGTCTTT GAGCAGAAGC ACGTTAAGAA GCCCGAAGGG CTTTTCAGAA 
ATCGGTTTGA AGTTAGAAAT GGTTTCCCAG GATGCCCTGA GGAGAACCAT CTTCCCTCTG 
GGGGGATTAA CGAAAGAGTT TGTAAAGAAA ATCGCTGCTG AGAATAGACT TCATCATGTG 
CTTCAGAAGA AAGAGAGCAT GGGCATGTGT TTCATCGGGA AGAGGAATTT TGAACATTTC 
CTTCTTCAGT ATCTGCAGCC TCGACCTGGT CACTTTATTT CCATAGAAGA CAATAAGGTT 
CTGGGAACAC ATAAAGGTTG GTTCCTGTAT ACCTTGGGCC AGAGAGCAAA CATAGGTGGC 
CTGAGAGAGC CCTGGTACGT GGTGGAGAAG GACAGCGTCA AGGGTGACGT GTTTGTGGCC 
CCCCGGACAG ACCACCCAGC CCTGTACAGG GACCTGCTGA GGACCAGCCG CGTGCACTGG 
ATTGCGGAGG AGCCTCCCGC AGCACTGGTC CGGGACAAGA TGATGGAGTG CCACTTCCGA 
TTCCGCCACC AGATGGCACT AGTGCCCTGT GTGCTGACCC TCAATCAAGA TGGCACCGTG 
TGGGTGACAG CTGTGCAGGC TGTGCGTGCC CTTGCCACAG GACAGTTTGC TGTGTTCTAC 
AAGGGGGACG AGTGCCTGGG CAGCGGGAAG ATCCTGCGGC TGGGGCCGTC TGCCTACACG 
CTCCAGAAGG GCCAGCGCAG AGCTGGGATG GCCACTGAGA GCCCCAGTGA CAGCCCAGAA 
GATGGTCCAG GCCTGAGTCC CTTGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005261735.1
CDS	389..1258
Translation	MLWIILGQMPLPQVTMQELPWKMKKSLSRSTLRSPKGFSEIGLKLEMVSQDALRRTIFPLGGLTKEFVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHFISIEDNKVLGTHKGWFLYTLGQRANIGGLREPWYVVEKDSVKGDVFVAPRTDHPALYRDLLRTSRVHWIAEEPPAALVRDKMMECHFRFRHQMALVPCVLTLNQDGTVWVTAVQAVRALATGQFAVFYKGDECLGSGKILRLGPSAYTLQKGQRRAGMATESPSDSPEDGPGLSPLL

Target ORF information:

RefSeq Version	XM_005261678.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005261678.1

ORF Insert Sequence:

ATGCTGTGGA TAATCTTGGG GCAGATGCCA TTGCCACAGG TCACTATGCA AGAACTTCCC 
TGGAAGATGA AGAAGTCTTT GAGCAGAAGC ACGTTAAGAA GCCCGAAGGG CTTTTCAGAA 
ATCGGTTTGA AGTTAGAAAT GGTTTCCCAG GATGCCCTGA GGAGAACCAT CTTCCCTCTG 
GGGGGATTAA CGAAAGAGTT TGTAAAGAAA ATCGCTGCTG AGAATAGACT TCATCATGTG 
CTTCAGAAGA AAGAGAGCAT GGGCATGTGT TTCATCGGGA AGAGGAATTT TGAACATTTC 
CTTCTTCAGT ATCTGCAGCC TCGACCTGGT CACTTTATTT CCATAGAAGA CAATAAGGTT 
CTGGGAACAC ATAAAGGTTG GTTCCTGTAT ACCTTGGGCC AGAGAGCAAA CATAGGTGGC 
CTGAGAGAGC CCTGGTACGT GGTGGAGAAG GACAGCGTCA AGGGTGACGT GTTTGTGGCC 
CCCCGGACAG ACCACCCAGC CCTGTACAGG GACCTGCTGA GGACCAGCCG CGTGCACTGG 
ATTGCGGAGG AGCCTCCCGC AGCACTGGTC CGGGACAAGA TGATGGAGTG CCACTTCCGA 
TTCCGCCACC AGATGGCACT AGTGCCCTGT GTGCTGACCC TCAATCAAGA TGGCACCGTG 
TGGGTGACAG CTGTGCAGGC TGTGCGTGCC CTTGCCACAG GACAGTTTGC TGTGTTCTAC 
AAGGGGGACG AGTGCCTGGG CAGCGGGAAG ATCCTGCGGC TGGGGCCGTC TGCCTACACG 
CTCCAGAAGG GCCAGCGCAG AGCTGGGATG GCCACTGAGA GCCCCAGTGA CAGCCCAGAA 
GATGGTCCAG GCCTGAGTCC CTTGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMU ( XM_011530275.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011528577.1 Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu53634
Clone ID Related Accession (Same CDS sequence)	XM_005261678.1 , XM_011530275.1
Accession Version	XM_011530275.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitochondrial tRNA-specific 2-thiouridylase 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGGA TAATCTTGGG GCAGATGCCA TTGCCACAGG TCACTATGCA AGAACTTCCC 
TGGAAGATGA AGAAGTCTTT GAGCAGAAGC ACGTTAAGAA GCCCGAAGGG CTTTTCAGAA 
ATCGGTTTGA AGTTAGAAAT GGTTTCCCAG GATGCCCTGA GGAGAACCAT CTTCCCTCTG 
GGGGGATTAA CGAAAGAGTT TGTAAAGAAA ATCGCTGCTG AGAATAGACT TCATCATGTG 
CTTCAGAAGA AAGAGAGCAT GGGCATGTGT TTCATCGGGA AGAGGAATTT TGAACATTTC 
CTTCTTCAGT ATCTGCAGCC TCGACCTGGT CACTTTATTT CCATAGAAGA CAATAAGGTT 
CTGGGAACAC ATAAAGGTTG GTTCCTGTAT ACCTTGGGCC AGAGAGCAAA CATAGGTGGC 
CTGAGAGAGC CCTGGTACGT GGTGGAGAAG GACAGCGTCA AGGGTGACGT GTTTGTGGCC 
CCCCGGACAG ACCACCCAGC CCTGTACAGG GACCTGCTGA GGACCAGCCG CGTGCACTGG 
ATTGCGGAGG AGCCTCCCGC AGCACTGGTC CGGGACAAGA TGATGGAGTG CCACTTCCGA 
TTCCGCCACC AGATGGCACT AGTGCCCTGT GTGCTGACCC TCAATCAAGA TGGCACCGTG 
TGGGTGACAG CTGTGCAGGC TGTGCGTGCC CTTGCCACAG GACAGTTTGC TGTGTTCTAC 
AAGGGGGACG AGTGCCTGGG CAGCGGGAAG ATCCTGCGGC TGGGGCCGTC TGCCTACACG 
CTCCAGAAGG GCCAGCGCAG AGCTGGGATG GCCACTGAGA GCCCCAGTGA CAGCCCAGAA 
GATGGTCCAG GCCTGAGTCC CTTGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011528577.1
CDS	345..1214
Translation	MLWIILGQMPLPQVTMQELPWKMKKSLSRSTLRSPKGFSEIGLKLEMVSQDALRRTIFPLGGLTKEFVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHFISIEDNKVLGTHKGWFLYTLGQRANIGGLREPWYVVEKDSVKGDVFVAPRTDHPALYRDLLRTSRVHWIAEEPPAALVRDKMMECHFRFRHQMALVPCVLTLNQDGTVWVTAVQAVRALATGQFAVFYKGDECLGSGKILRLGPSAYTLQKGQRRAGMATESPSDSPEDGPGLSPLL

Target ORF information:

RefSeq Version	XM_011530275.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530275.1

ORF Insert Sequence:

ATGCTGTGGA TAATCTTGGG GCAGATGCCA TTGCCACAGG TCACTATGCA AGAACTTCCC 
TGGAAGATGA AGAAGTCTTT GAGCAGAAGC ACGTTAAGAA GCCCGAAGGG CTTTTCAGAA 
ATCGGTTTGA AGTTAGAAAT GGTTTCCCAG GATGCCCTGA GGAGAACCAT CTTCCCTCTG 
GGGGGATTAA CGAAAGAGTT TGTAAAGAAA ATCGCTGCTG AGAATAGACT TCATCATGTG 
CTTCAGAAGA AAGAGAGCAT GGGCATGTGT TTCATCGGGA AGAGGAATTT TGAACATTTC 
CTTCTTCAGT ATCTGCAGCC TCGACCTGGT CACTTTATTT CCATAGAAGA CAATAAGGTT 
CTGGGAACAC ATAAAGGTTG GTTCCTGTAT ACCTTGGGCC AGAGAGCAAA CATAGGTGGC 
CTGAGAGAGC CCTGGTACGT GGTGGAGAAG GACAGCGTCA AGGGTGACGT GTTTGTGGCC 
CCCCGGACAG ACCACCCAGC CCTGTACAGG GACCTGCTGA GGACCAGCCG CGTGCACTGG 
ATTGCGGAGG AGCCTCCCGC AGCACTGGTC CGGGACAAGA TGATGGAGTG CCACTTCCGA 
TTCCGCCACC AGATGGCACT AGTGCCCTGT GTGCTGACCC TCAATCAAGA TGGCACCGTG 
TGGGTGACAG CTGTGCAGGC TGTGCGTGCC CTTGCCACAG GACAGTTTGC TGTGTTCTAC 
AAGGGGGACG AGTGCCTGGG CAGCGGGAAG ATCCTGCGGC TGGGGCCGTC TGCCTACACG 
CTCCAGAAGG GCCAGCGCAG AGCTGGGATG GCCACTGAGA GCCCCAGTGA CAGCCCAGAA 
GATGGTCCAG GCCTGAGTCC CTTGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMU ( XM_005261681.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005261738.1 Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu53635
Clone ID Related Accession (Same CDS sequence)	XM_005261681.1
Accession Version	XM_005261681.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitochondrial tRNA-specific 2-thiouridylase 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGGA TAATCTTGGG GCAGATGCCA TTGCCACAGG TCACTATGCA AGAACTTCCC 
TGGAAGATGA AGAAGTCTTT GAGCAGAAGC ACGTTAAGAA GCCCGAAGGG CTTTTCAGAA 
ATCGGTTTGA AGTTAGAAAT GGTTTCCCAG GATGCCCTGA GGAGAACCAT CTTCCCTCTG 
GGGGGATTAA CGAAAGAGTT TGTAAAGAAA ATCGCTGCTG AGAATAGACT TCATCATGTG 
CTTCAGAAGA AAGAGAGCAT GGGCATGTGT TTCATCGGGA AGAGGAATTT TGAACATTTC 
CTTCTTCAGT ATCTGCAGCC TCGACCTGGT CACTTTATTT CCATAGAAGA CAATAAGGTT 
CTGGGAACAC ATAAAGGTTG GTTCCTGTAT ACCTTGGGCC AGAGAGCAAA CATAGGTGGC 
CTGAGAGAGC CCTGGTACGT GGTGGAGAAG GACAGCGTCA AGGGTGACGT GTTTGTGGCC 
CCCCGGACAG ACCACCCAGC CCTGTACAGG GACCTGCTGA GGACCAGCCG CGTGCACTGG 
ATTGCGGAGG AGCCTCCCGC AGCACTGGTC CGGGACAAGA TGATGGAGTG CCACTTCCGA 
TTCCGCCACC AGATGGCACT AGTTTGCTGT GTTCTACAAG GGGGACGAGT GCCTGGGCAG 
CGGGAAGATC CTGCGGCTGG GGCCGTCTGC CTACACGCTC CAGAAGGGCC AGCGCAGAGC 
TGGGATGGCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005261738.1
CDS	389..1123
Translation	MLWIILGQMPLPQVTMQELPWKMKKSLSRSTLRSPKGFSEIGLKLEMVSQDALRRTIFPLGGLTKEFVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHFISIEDNKVLGTHKGWFLYTLGQRANIGGLREPWYVVEKDSVKGDVFVAPRTDHPALYRDLLRTSRVHWIAEEPPAALVRDKMMECHFRFRHQMALVCCVLQGGRVPGQREDPAAGAVCLHAPEGPAQSWDGH

Target ORF information:

RefSeq Version	XM_005261681.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005261681.1

ORF Insert Sequence:

ATGCTGTGGA TAATCTTGGG GCAGATGCCA TTGCCACAGG TCACTATGCA AGAACTTCCC 
TGGAAGATGA AGAAGTCTTT GAGCAGAAGC ACGTTAAGAA GCCCGAAGGG CTTTTCAGAA 
ATCGGTTTGA AGTTAGAAAT GGTTTCCCAG GATGCCCTGA GGAGAACCAT CTTCCCTCTG 
GGGGGATTAA CGAAAGAGTT TGTAAAGAAA ATCGCTGCTG AGAATAGACT TCATCATGTG 
CTTCAGAAGA AAGAGAGCAT GGGCATGTGT TTCATCGGGA AGAGGAATTT TGAACATTTC 
CTTCTTCAGT ATCTGCAGCC TCGACCTGGT CACTTTATTT CCATAGAAGA CAATAAGGTT 
CTGGGAACAC ATAAAGGTTG GTTCCTGTAT ACCTTGGGCC AGAGAGCAAA CATAGGTGGC 
CTGAGAGAGC CCTGGTACGT GGTGGAGAAG GACAGCGTCA AGGGTGACGT GTTTGTGGCC 
CCCCGGACAG ACCACCCAGC CCTGTACAGG GACCTGCTGA GGACCAGCCG CGTGCACTGG 
ATTGCGGAGG AGCCTCCCGC AGCACTGGTC CGGGACAAGA TGATGGAGTG CCACTTCCGA 
TTCCGCCACC AGATGGCACT AGTTTGCTGT GTTCTACAAG GGGGACGAGT GCCTGGGCAG 
CGGGAAGATC CTGCGGCTGG GGCCGTCTGC CTACACGCTC CAGAAGGGCC AGCGCAGAGC 
TGGGATGGCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMU ( XM_011530271.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011528573.1 Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu69627
Clone ID Related Accession (Same CDS sequence)	XM_011530271.2
Accession Version	XM_011530271.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitochondrial tRNA-specific 2-thiouridylase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530271.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAACT GGGACTCACT GGATGAACAT GGGGTCTGTA CTGCCGACAA AGACTGTGAA 
GATGCTTACA GAGTTTGCCA GATCTTAGAC ATCCCTTTCC ATCAAGTGTC CTACGTAAAG 
GAGTATTGGA ATGATGTGTT CAGTGACTTT TTGAATGAGT ATGAAAAAGG AAGGACTCCC 
AATCCTGACA TAGTTTGCAA CAAGCACATC AAATTTAGTT GCTTTTTTCA TTATGCTGTG 
GATAATCTTG GGGCAGATGC CATTGCCACA GGTCACTATG CAAGAACTTC CCTGGAAGAT 
GAAGAAGTCT TTGAGCAGAA GCACGTTAAG AAGCCCGAAG GGCTTTTCAG AAATCGGTTT 
GAAGTTAGAA ATGCGGTAAA ACTCCTCCAG GCAGCTGACA GCTTTAAAGA CCAGACCTTC 
TTTCTCAGCC AGGTTTCCCA GGATGCCCTG AGGAGAACCA TCTTCCCTCT GGGGGGATTA 
ACGAAAGAGT TTGTAAAGAA AATCGCTGCT GAGAATAGAC TTCATCATGT GCTTCAGAAG 
AAAGAGAGCA TGGGCATGTG TTTCATCGGG AAGAGGAATT TTGAACATTT CCTTCTTCAG 
TATCTGCAGC CTCGACCTGG TCACTTTATT TCCATAGAAG ACAATAAGGT TCTGGGAACA 
CATAAAGGTT GGTTCCTGTA TACCTTGGGC CAGAGAGCAA ACATAGGTGG CCTGAGAGAG 
CCCTGGTACG TGGTGGAGAA GGACAGCGTC AAGGGTGACG TGTTTGTGGC CCCCCGGACA 
GACCACCCAG CCCTGTACAG GGACCTGCTG AGGACCAGCC GCGTGCACTG GATTGCGGAG 
GAGCCTCCCG CAGCACTGGT CCGGGACAAG ATGATGGAGT GCCACTTCCG ATTCCGCCAC 
CAGATGGCAC TAGTGCCCTG TGTGCTGACC CTCAATCAAG ATGGCACCGT GTGGGTGACA 
GCTGTGCAGG CTGTGCGTGC CCTTGCCACA GGACAGTTTG CTGTGTTCTA CAAGGGGGAC 
GAGTGCCTGG GCAGCGGGAA GATCCTGCGG CTGGGGCCGT CTGCCTACAC GCTCCAGAAG 
GGCCAGCGCA GAGCTGGGAT GGCCACTGAG AGCCCCAGTG ACAGCCCAGA AGATGGTCCA 
GGCCTGAGTC CCTTGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011528573.1
CDS	233..1393
Translation	MKNWDSLDEHGVCTADKDCEDAYRVCQILDIPFHQVSYVKEYWNDVFSDFLNEYEKGRTPNPDIVCNKHIKFSCFFHYAVDNLGADAIATGHYARTSLEDEEVFEQKHVKKPEGLFRNRFEVRNAVKLLQAADSFKDQTFFLSQVSQDALRRTIFPLGGLTKEFVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHFISIEDNKVLGTHKGWFLYTLGQRANIGGLREPWYVVEKDSVKGDVFVAPRTDHPALYRDLLRTSRVHWIAEEPPAALVRDKMMECHFRFRHQMALVPCVLTLNQDGTVWVTAVQAVRALATGQFAVFYKGDECLGSGKILRLGPSAYTLQKGQRRAGMATESPSDSPEDGPGLSPLL

Target ORF information:

RefSeq Version	XM_011530271.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530271.2

ORF Insert Sequence:

ATGAAGAACT GGGACTCACT GGATGAACAT GGGGTCTGTA CTGCCGACAA AGACTGTGAA 
GATGCTTACA GAGTTTGCCA GATCTTAGAC ATCCCTTTCC ATCAAGTGTC CTACGTAAAG 
GAGTATTGGA ATGATGTGTT CAGTGACTTT TTGAATGAGT ATGAAAAAGG AAGGACTCCC 
AATCCTGACA TAGTTTGCAA CAAGCACATC AAATTTAGTT GCTTTTTTCA TTATGCTGTG 
GATAATCTTG GGGCAGATGC CATTGCCACA GGTCACTATG CAAGAACTTC CCTGGAAGAT 
GAAGAAGTCT TTGAGCAGAA GCACGTTAAG AAGCCCGAAG GGCTTTTCAG AAATCGGTTT 
GAAGTTAGAA ATGCGGTAAA ACTCCTCCAG GCAGCTGACA GCTTTAAAGA CCAGACCTTC 
TTTCTCAGCC AGGTTTCCCA GGATGCCCTG AGGAGAACCA TCTTCCCTCT GGGGGGATTA 
ACGAAAGAGT TTGTAAAGAA AATCGCTGCT GAGAATAGAC TTCATCATGT GCTTCAGAAG 
AAAGAGAGCA TGGGCATGTG TTTCATCGGG AAGAGGAATT TTGAACATTT CCTTCTTCAG 
TATCTGCAGC CTCGACCTGG TCACTTTATT TCCATAGAAG ACAATAAGGT TCTGGGAACA 
CATAAAGGTT GGTTCCTGTA TACCTTGGGC CAGAGAGCAA ACATAGGTGG CCTGAGAGAG 
CCCTGGTACG TGGTGGAGAA GGACAGCGTC AAGGGTGACG TGTTTGTGGC CCCCCGGACA 
GACCACCCAG CCCTGTACAG GGACCTGCTG AGGACCAGCC GCGTGCACTG GATTGCGGAG 
GAGCCTCCCG CAGCACTGGT CCGGGACAAG ATGATGGAGT GCCACTTCCG ATTCCGCCAC 
CAGATGGCAC TAGTGCCCTG TGTGCTGACC CTCAATCAAG ATGGCACCGT GTGGGTGACA 
GCTGTGCAGG CTGTGCGTGC CCTTGCCACA GGACAGTTTG CTGTGTTCTA CAAGGGGGAC 
GAGTGCCTGG GCAGCGGGAA GATCCTGCGG CTGGGGCCGT CTGCCTACAC GCTCCAGAAG 
GGCCAGCGCA GAGCTGGGAT GGCCACTGAG AGCCCCAGTG ACAGCCCAGA AGATGGTCCA 
GGCCTGAGTC CCTTGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMU ( XM_011530272.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011528574.1 Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu69628
Clone ID Related Accession (Same CDS sequence)	XM_011530272.1 , XM_011530272.2
Accession Version	XM_011530272.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mitochondrial tRNA-specific 2-thiouridylase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCCT TGCGGCACGT CGTGTGCGCC CTGTCCGGCG GCGTGGACAG CGCCGTGGCC 
GCGCTGCTGC TGAGGCGGAG AGGTTACCAG GTGACAGGGG TGTTTATGAA GAACTGGGAC 
TCACTGGATG AACATGGGGT CTGTACTGCC GACAAAGACT GTGAAGATGC TTACAGAGTT 
TGCCAGATCT TAGACATCCC TTTCCATCAA GTGTCCTACG TAAAGGAGTA TTGGAATGAT 
GTGTTCAGTG ACTTTTTGAA TGAGTATGAA AAAGGAAGGA CTCCCAATCC TGACATAGTT 
TGCAACAAGC ACATCAAATT TAGTTGCTTT TTTCATTATG CTGTGGATAA TCTTGGGGCA 
GATGCCATTG CCACAGGTCA CTATGCAAGA ACTTCCCTGG AAGATGAAGA AGTCTTTGAG 
CAGAAGCACG TTAAGAAGCC CGAAGGGCTT TTCAGAAATC GGTTTGAAGT TAGAAATGCG 
GTAAAACTCC TCCAGGCAGC TGACAGCTTT AAAGACCAGA CCTTCTTTCT CAGCCAGGTT 
TCCCAGGATG CCCTGAGGAG AACCATCTTC CCTCTGGGGG GATTAACGAA AGAGTTTGTA 
AAGAAAATCG CTGCTGAGAA TAGACTTCAT CATGTGCTTC AGAAGAAAGA GAGCATGGGC 
ATGTGTTTCA TCGGGAAGAG GAATTTTGAA CATTTCCTTC TTCAGTATCT GCAGCCTCGA 
CCTGGTCACT TTATTTCCAT AGAAGACAAT AAGGTTCTGG GAACACATAA AGGCCCCCCG 
GACAGACCAC CCAGCCCTGT ACAGGGACCT GCTGAGGACC AGCCGCGTGC ACTGGATTGC 
GGAGGAGCCT CCCGCAGCAC TGGTCCGGGA CAAGATGATG GAGTGCCACT TCCGATTCCG 
CCACCAGATG GCACTAGTGC CCTGTGTGCT GACCCTCAAT CAAGATGGCA CCGTGTGGGT 
GACAGCTGTG CAGGCTGTGC GTGCCCTTGC CACAGGACAG TTTGCTGTGT TCTACAAGGG 
GGACGAGTGC CTGGGCAGCG GGAAGATCCT GCGGCTGGGG CCGTCTGCCT ACACGCTCCA 
GAAGGGCCAG CGCAGAGCTG GGATGGCCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011528574.1
CDS	52..1164
Translation	MQALRHVVCALSGGVDSAVAALLLRRRGYQVTGVFMKNWDSLDEHGVCTADKDCEDAYRVCQILDIPFHQVSYVKEYWNDVFSDFLNEYEKGRTPNPDIVCNKHIKFSCFFHYAVDNLGADAIATGHYARTSLEDEEVFEQKHVKKPEGLFRNRFEVRNAVKLLQAADSFKDQTFFLSQVSQDALRRTIFPLGGLTKEFVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHFISIEDNKVLGTHKGPPDRPPSPVQGPAEDQPRALDCGGASRSTGPGQDDGVPLPIPPPDGTSALCADPQSRWHRVGDSCAGCACPCHRTVCCVLQGGRVPGQREDPAAGAVCLHAPEGPAQSWDGH

Target ORF information:

RefSeq Version	XM_011530272.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530272.1

ORF Insert Sequence:

ATGCAGGCCT TGCGGCACGT CGTGTGCGCC CTGTCCGGCG GCGTGGACAG CGCCGTGGCC 
GCGCTGCTGC TGAGGCGGAG AGGTTACCAG GTGACAGGGG TGTTTATGAA GAACTGGGAC 
TCACTGGATG AACATGGGGT CTGTACTGCC GACAAAGACT GTGAAGATGC TTACAGAGTT 
TGCCAGATCT TAGACATCCC TTTCCATCAA GTGTCCTACG TAAAGGAGTA TTGGAATGAT 
GTGTTCAGTG ACTTTTTGAA TGAGTATGAA AAAGGAAGGA CTCCCAATCC TGACATAGTT 
TGCAACAAGC ACATCAAATT TAGTTGCTTT TTTCATTATG CTGTGGATAA TCTTGGGGCA 
GATGCCATTG CCACAGGTCA CTATGCAAGA ACTTCCCTGG AAGATGAAGA AGTCTTTGAG 
CAGAAGCACG TTAAGAAGCC CGAAGGGCTT TTCAGAAATC GGTTTGAAGT TAGAAATGCG 
GTAAAACTCC TCCAGGCAGC TGACAGCTTT AAAGACCAGA CCTTCTTTCT CAGCCAGGTT 
TCCCAGGATG CCCTGAGGAG AACCATCTTC CCTCTGGGGG GATTAACGAA AGAGTTTGTA 
AAGAAAATCG CTGCTGAGAA TAGACTTCAT CATGTGCTTC AGAAGAAAGA GAGCATGGGC 
ATGTGTTTCA TCGGGAAGAG GAATTTTGAA CATTTCCTTC TTCAGTATCT GCAGCCTCGA 
CCTGGTCACT TTATTTCCAT AGAAGACAAT AAGGTTCTGG GAACACATAA AGGCCCCCCG 
GACAGACCAC CCAGCCCTGT ACAGGGACCT GCTGAGGACC AGCCGCGTGC ACTGGATTGC 
GGAGGAGCCT CCCGCAGCAC TGGTCCGGGA CAAGATGATG GAGTGCCACT TCCGATTCCG 
CCACCAGATG GCACTAGTGC CCTGTGTGCT GACCCTCAAT CAAGATGGCA CCGTGTGGGT 
GACAGCTGTG CAGGCTGTGC GTGCCCTTGC CACAGGACAG TTTGCTGTGT TCTACAAGGG 
GGACGAGTGC CTGGGCAGCG GGAAGATCCT GCGGCTGGGG CCGTCTGCCT ACACGCTCCA 
GAAGGGCCAG CGCAGAGCTG GGATGGCCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMU ( XM_011530272.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011528574.1 Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu69628
Clone ID Related Accession (Same CDS sequence)	XM_011530272.1 , XM_011530272.2
Accession Version	XM_011530272.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitochondrial tRNA-specific 2-thiouridylase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011530272.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCCT TGCGGCACGT CGTGTGCGCC CTGTCCGGCG GCGTGGACAG CGCCGTGGCC 
GCGCTGCTGC TGAGGCGGAG AGGTTACCAG GTGACAGGGG TGTTTATGAA GAACTGGGAC 
TCACTGGATG AACATGGGGT CTGTACTGCC GACAAAGACT GTGAAGATGC TTACAGAGTT 
TGCCAGATCT TAGACATCCC TTTCCATCAA GTGTCCTACG TAAAGGAGTA TTGGAATGAT 
GTGTTCAGTG ACTTTTTGAA TGAGTATGAA AAAGGAAGGA CTCCCAATCC TGACATAGTT 
TGCAACAAGC ACATCAAATT TAGTTGCTTT TTTCATTATG CTGTGGATAA TCTTGGGGCA 
GATGCCATTG CCACAGGTCA CTATGCAAGA ACTTCCCTGG AAGATGAAGA AGTCTTTGAG 
CAGAAGCACG TTAAGAAGCC CGAAGGGCTT TTCAGAAATC GGTTTGAAGT TAGAAATGCG 
GTAAAACTCC TCCAGGCAGC TGACAGCTTT AAAGACCAGA CCTTCTTTCT CAGCCAGGTT 
TCCCAGGATG CCCTGAGGAG AACCATCTTC CCTCTGGGGG GATTAACGAA AGAGTTTGTA 
AAGAAAATCG CTGCTGAGAA TAGACTTCAT CATGTGCTTC AGAAGAAAGA GAGCATGGGC 
ATGTGTTTCA TCGGGAAGAG GAATTTTGAA CATTTCCTTC TTCAGTATCT GCAGCCTCGA 
CCTGGTCACT TTATTTCCAT AGAAGACAAT AAGGTTCTGG GAACACATAA AGGCCCCCCG 
GACAGACCAC CCAGCCCTGT ACAGGGACCT GCTGAGGACC AGCCGCGTGC ACTGGATTGC 
GGAGGAGCCT CCCGCAGCAC TGGTCCGGGA CAAGATGATG GAGTGCCACT TCCGATTCCG 
CCACCAGATG GCACTAGTGC CCTGTGTGCT GACCCTCAAT CAAGATGGCA CCGTGTGGGT 
GACAGCTGTG CAGGCTGTGC GTGCCCTTGC CACAGGACAG TTTGCTGTGT TCTACAAGGG 
GGACGAGTGC CTGGGCAGCG GGAAGATCCT GCGGCTGGGG CCGTCTGCCT ACACGCTCCA 
GAAGGGCCAG CGCAGAGCTG GGATGGCCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011528574.1
CDS	47..1159
Translation	MQALRHVVCALSGGVDSAVAALLLRRRGYQVTGVFMKNWDSLDEHGVCTADKDCEDAYRVCQILDIPFHQVSYVKEYWNDVFSDFLNEYEKGRTPNPDIVCNKHIKFSCFFHYAVDNLGADAIATGHYARTSLEDEEVFEQKHVKKPEGLFRNRFEVRNAVKLLQAADSFKDQTFFLSQVSQDALRRTIFPLGGLTKEFVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHFISIEDNKVLGTHKGPPDRPPSPVQGPAEDQPRALDCGGASRSTGPGQDDGVPLPIPPPDGTSALCADPQSRWHRVGDSCAGCACPCHRTVCCVLQGGRVPGQREDPAAGAVCLHAPEGPAQSWDGH

Target ORF information:

RefSeq Version	XM_011530272.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530272.2

ORF Insert Sequence:

ATGCAGGCCT TGCGGCACGT CGTGTGCGCC CTGTCCGGCG GCGTGGACAG CGCCGTGGCC 
GCGCTGCTGC TGAGGCGGAG AGGTTACCAG GTGACAGGGG TGTTTATGAA GAACTGGGAC 
TCACTGGATG AACATGGGGT CTGTACTGCC GACAAAGACT GTGAAGATGC TTACAGAGTT 
TGCCAGATCT TAGACATCCC TTTCCATCAA GTGTCCTACG TAAAGGAGTA TTGGAATGAT 
GTGTTCAGTG ACTTTTTGAA TGAGTATGAA AAAGGAAGGA CTCCCAATCC TGACATAGTT 
TGCAACAAGC ACATCAAATT TAGTTGCTTT TTTCATTATG CTGTGGATAA TCTTGGGGCA 
GATGCCATTG CCACAGGTCA CTATGCAAGA ACTTCCCTGG AAGATGAAGA AGTCTTTGAG 
CAGAAGCACG TTAAGAAGCC CGAAGGGCTT TTCAGAAATC GGTTTGAAGT TAGAAATGCG 
GTAAAACTCC TCCAGGCAGC TGACAGCTTT AAAGACCAGA CCTTCTTTCT CAGCCAGGTT 
TCCCAGGATG CCCTGAGGAG AACCATCTTC CCTCTGGGGG GATTAACGAA AGAGTTTGTA 
AAGAAAATCG CTGCTGAGAA TAGACTTCAT CATGTGCTTC AGAAGAAAGA GAGCATGGGC 
ATGTGTTTCA TCGGGAAGAG GAATTTTGAA CATTTCCTTC TTCAGTATCT GCAGCCTCGA 
CCTGGTCACT TTATTTCCAT AGAAGACAAT AAGGTTCTGG GAACACATAA AGGCCCCCCG 
GACAGACCAC CCAGCCCTGT ACAGGGACCT GCTGAGGACC AGCCGCGTGC ACTGGATTGC 
GGAGGAGCCT CCCGCAGCAC TGGTCCGGGA CAAGATGATG GAGTGCCACT TCCGATTCCG 
CCACCAGATG GCACTAGTGC CCTGTGTGCT GACCCTCAAT CAAGATGGCA CCGTGTGGGT 
GACAGCTGTG CAGGCTGTGC GTGCCCTTGC CACAGGACAG TTTGCTGTGT TCTACAAGGG 
GGACGAGTGC CTGGGCAGCG GGAAGATCCT GCGGCTGGGG CCGTCTGCCT ACACGCTCCA 
GAAGGGCCAG CGCAGAGCTG GGATGGCCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMU ( XM_011530273.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011528575.1 Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu69629
Clone ID Related Accession (Same CDS sequence)	XM_011530273.1 , XM_011530273.2
Accession Version	XM_011530273.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mitochondrial tRNA-specific 2-thiouridylase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCCT TGCGGCACGT CGTGTGCGCC CTGTCCGGCG GCGTGGACAG CGCCGTGGCC 
GCGCTGCTGC TGAGGCGGAG AGGTTACCAG GTGACAGGGG TGTTTATGAA GAACTGGGAC 
TCACTGGATG AACATGGGGT CTGTACTGCC GACAAAGACT GTGAAGATGC TTACAGAGTT 
TGCCAGATCT TAGACATCCC TTTCCATCAA GTGTCCTACG TAAAGGAGTA TTGGAATGAT 
GTGTTCAGTG ACTTTTTGAA TGAGTATGAA AAAGGAAGGA CTCCCAATCC TGACATAGTT 
TGCAACAAGC ACATCAAATT TAGTTGCTTT TTTCATTATG CTGTGGATAA TCTTGGGGCA 
GATGCCATTG CCACAGGTCA CTATGCAAGA ACTTCCCTGG AAGATGAAGA AGTCTTTGAG 
CAGAAGCACG TTAAGAAGCC CGAAGGGCTT TTCAGAAATC GGTTTGAAGT TAGAAATGCG 
GTAAAACTCC TCCAGGCAGC TGACAGCTTT AAAGACCAGA CCTTCTTTCT CAGCCAGGTT 
TCCCAGGATG CCCTGAGGAG AACCATCTTC CCTCTGGGGG GATTAACGAA AGAGTTTGTA 
AAGAAAATCG CTGCTGAGAA TAGACTTCAT CATGTGCTTC AGAAGAAAGA GAGCATGGGC 
ATGTGTTTCA TCGGGAAGAG GAATTTTGAA CATTTCCTTC TTCAGTATCT GCAGCCTCGA 
CCTGGTCACT TTATTTCCAT AGAAGACAAT AAGGTTCTGG GAACACATAA AGGCCCCCCG 
GACAGACCAC CCAGCCCTGT ACAGGGACCT GCTGAGGACC AGCCGCGTGC ACTGGATTGC 
GGAGGAGCCT CCCGCAGCAC TGGTCCGGGA CAAGATGATG GAGTGCCACT TCCGATTCCG 
CCACCAGATG GCACTAGTTT GCTGTGTTCT ACAAGGGGGA CGAGTGCCTG GGCAGCGGGA 
AGATCCTGCG GCTGGGGCCG TCTGCCTACA CGCTCCAGAA GGGCCAGCGC AGAGCTGGGA 
TGGCCACTGA GAGCCCCAGT GACAGCCCAG AAGATGGTCC AGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011528575.1
CDS	52..1119
Translation	MQALRHVVCALSGGVDSAVAALLLRRRGYQVTGVFMKNWDSLDEHGVCTADKDCEDAYRVCQILDIPFHQVSYVKEYWNDVFSDFLNEYEKGRTPNPDIVCNKHIKFSCFFHYAVDNLGADAIATGHYARTSLEDEEVFEQKHVKKPEGLFRNRFEVRNAVKLLQAADSFKDQTFFLSQVSQDALRRTIFPLGGLTKEFVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHFISIEDNKVLGTHKGPPDRPPSPVQGPAEDQPRALDCGGASRSTGPGQDDGVPLPIPPPDGTSLLCSTRGTSAWAAGRSCGWGRLPTRSRRASAELGWPLRAPVTAQKMVQA

Target ORF information:

RefSeq Version	XM_011530273.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530273.1

ORF Insert Sequence:

ATGCAGGCCT TGCGGCACGT CGTGTGCGCC CTGTCCGGCG GCGTGGACAG CGCCGTGGCC 
GCGCTGCTGC TGAGGCGGAG AGGTTACCAG GTGACAGGGG TGTTTATGAA GAACTGGGAC 
TCACTGGATG AACATGGGGT CTGTACTGCC GACAAAGACT GTGAAGATGC TTACAGAGTT 
TGCCAGATCT TAGACATCCC TTTCCATCAA GTGTCCTACG TAAAGGAGTA TTGGAATGAT 
GTGTTCAGTG ACTTTTTGAA TGAGTATGAA AAAGGAAGGA CTCCCAATCC TGACATAGTT 
TGCAACAAGC ACATCAAATT TAGTTGCTTT TTTCATTATG CTGTGGATAA TCTTGGGGCA 
GATGCCATTG CCACAGGTCA CTATGCAAGA ACTTCCCTGG AAGATGAAGA AGTCTTTGAG 
CAGAAGCACG TTAAGAAGCC CGAAGGGCTT TTCAGAAATC GGTTTGAAGT TAGAAATGCG 
GTAAAACTCC TCCAGGCAGC TGACAGCTTT AAAGACCAGA CCTTCTTTCT CAGCCAGGTT 
TCCCAGGATG CCCTGAGGAG AACCATCTTC CCTCTGGGGG GATTAACGAA AGAGTTTGTA 
AAGAAAATCG CTGCTGAGAA TAGACTTCAT CATGTGCTTC AGAAGAAAGA GAGCATGGGC 
ATGTGTTTCA TCGGGAAGAG GAATTTTGAA CATTTCCTTC TTCAGTATCT GCAGCCTCGA 
CCTGGTCACT TTATTTCCAT AGAAGACAAT AAGGTTCTGG GAACACATAA AGGCCCCCCG 
GACAGACCAC CCAGCCCTGT ACAGGGACCT GCTGAGGACC AGCCGCGTGC ACTGGATTGC 
GGAGGAGCCT CCCGCAGCAC TGGTCCGGGA CAAGATGATG GAGTGCCACT TCCGATTCCG 
CCACCAGATG GCACTAGTTT GCTGTGTTCT ACAAGGGGGA CGAGTGCCTG GGCAGCGGGA 
AGATCCTGCG GCTGGGGCCG TCTGCCTACA CGCTCCAGAA GGGCCAGCGC AGAGCTGGGA 
TGGCCACTGA GAGCCCCAGT GACAGCCCAG AAGATGGTCC AGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMU ( XM_011530273.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011528575.1 Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu69629
Clone ID Related Accession (Same CDS sequence)	XM_011530273.1 , XM_011530273.2
Accession Version	XM_011530273.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitochondrial tRNA-specific 2-thiouridylase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011530273.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCCT TGCGGCACGT CGTGTGCGCC CTGTCCGGCG GCGTGGACAG CGCCGTGGCC 
GCGCTGCTGC TGAGGCGGAG AGGTTACCAG GTGACAGGGG TGTTTATGAA GAACTGGGAC 
TCACTGGATG AACATGGGGT CTGTACTGCC GACAAAGACT GTGAAGATGC TTACAGAGTT 
TGCCAGATCT TAGACATCCC TTTCCATCAA GTGTCCTACG TAAAGGAGTA TTGGAATGAT 
GTGTTCAGTG ACTTTTTGAA TGAGTATGAA AAAGGAAGGA CTCCCAATCC TGACATAGTT 
TGCAACAAGC ACATCAAATT TAGTTGCTTT TTTCATTATG CTGTGGATAA TCTTGGGGCA 
GATGCCATTG CCACAGGTCA CTATGCAAGA ACTTCCCTGG AAGATGAAGA AGTCTTTGAG 
CAGAAGCACG TTAAGAAGCC CGAAGGGCTT TTCAGAAATC GGTTTGAAGT TAGAAATGCG 
GTAAAACTCC TCCAGGCAGC TGACAGCTTT AAAGACCAGA CCTTCTTTCT CAGCCAGGTT 
TCCCAGGATG CCCTGAGGAG AACCATCTTC CCTCTGGGGG GATTAACGAA AGAGTTTGTA 
AAGAAAATCG CTGCTGAGAA TAGACTTCAT CATGTGCTTC AGAAGAAAGA GAGCATGGGC 
ATGTGTTTCA TCGGGAAGAG GAATTTTGAA CATTTCCTTC TTCAGTATCT GCAGCCTCGA 
CCTGGTCACT TTATTTCCAT AGAAGACAAT AAGGTTCTGG GAACACATAA AGGCCCCCCG 
GACAGACCAC CCAGCCCTGT ACAGGGACCT GCTGAGGACC AGCCGCGTGC ACTGGATTGC 
GGAGGAGCCT CCCGCAGCAC TGGTCCGGGA CAAGATGATG GAGTGCCACT TCCGATTCCG 
CCACCAGATG GCACTAGTTT GCTGTGTTCT ACAAGGGGGA CGAGTGCCTG GGCAGCGGGA 
AGATCCTGCG GCTGGGGCCG TCTGCCTACA CGCTCCAGAA GGGCCAGCGC AGAGCTGGGA 
TGGCCACTGA GAGCCCCAGT GACAGCCCAG AAGATGGTCC AGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011528575.1
CDS	47..1114
Translation	MQALRHVVCALSGGVDSAVAALLLRRRGYQVTGVFMKNWDSLDEHGVCTADKDCEDAYRVCQILDIPFHQVSYVKEYWNDVFSDFLNEYEKGRTPNPDIVCNKHIKFSCFFHYAVDNLGADAIATGHYARTSLEDEEVFEQKHVKKPEGLFRNRFEVRNAVKLLQAADSFKDQTFFLSQVSQDALRRTIFPLGGLTKEFVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHFISIEDNKVLGTHKGPPDRPPSPVQGPAEDQPRALDCGGASRSTGPGQDDGVPLPIPPPDGTSLLCSTRGTSAWAAGRSCGWGRLPTRSRRASAELGWPLRAPVTAQKMVQA

Target ORF information:

RefSeq Version	XM_011530273.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530273.2

ORF Insert Sequence:

ATGCAGGCCT TGCGGCACGT CGTGTGCGCC CTGTCCGGCG GCGTGGACAG CGCCGTGGCC 
GCGCTGCTGC TGAGGCGGAG AGGTTACCAG GTGACAGGGG TGTTTATGAA GAACTGGGAC 
TCACTGGATG AACATGGGGT CTGTACTGCC GACAAAGACT GTGAAGATGC TTACAGAGTT 
TGCCAGATCT TAGACATCCC TTTCCATCAA GTGTCCTACG TAAAGGAGTA TTGGAATGAT 
GTGTTCAGTG ACTTTTTGAA TGAGTATGAA AAAGGAAGGA CTCCCAATCC TGACATAGTT 
TGCAACAAGC ACATCAAATT TAGTTGCTTT TTTCATTATG CTGTGGATAA TCTTGGGGCA 
GATGCCATTG CCACAGGTCA CTATGCAAGA ACTTCCCTGG AAGATGAAGA AGTCTTTGAG 
CAGAAGCACG TTAAGAAGCC CGAAGGGCTT TTCAGAAATC GGTTTGAAGT TAGAAATGCG 
GTAAAACTCC TCCAGGCAGC TGACAGCTTT AAAGACCAGA CCTTCTTTCT CAGCCAGGTT 
TCCCAGGATG CCCTGAGGAG AACCATCTTC CCTCTGGGGG GATTAACGAA AGAGTTTGTA 
AAGAAAATCG CTGCTGAGAA TAGACTTCAT CATGTGCTTC AGAAGAAAGA GAGCATGGGC 
ATGTGTTTCA TCGGGAAGAG GAATTTTGAA CATTTCCTTC TTCAGTATCT GCAGCCTCGA 
CCTGGTCACT TTATTTCCAT AGAAGACAAT AAGGTTCTGG GAACACATAA AGGCCCCCCG 
GACAGACCAC CCAGCCCTGT ACAGGGACCT GCTGAGGACC AGCCGCGTGC ACTGGATTGC 
GGAGGAGCCT CCCGCAGCAC TGGTCCGGGA CAAGATGATG GAGTGCCACT TCCGATTCCG 
CCACCAGATG GCACTAGTTT GCTGTGTTCT ACAAGGGGGA CGAGTGCCTG GGCAGCGGGA 
AGATCCTGCG GCTGGGGCCG TCTGCCTACA CGCTCCAGAA GGGCCAGCGC AGAGCTGGGA 
TGGCCACTGA GAGCCCCAGT GACAGCCCAG AAGATGGTCC AGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMU ( XM_024452260.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308028.1 Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X4, mRNA.

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CloneID	OHu105271
Clone ID Related Accession (Same CDS sequence)	XM_024452260.1
Accession Version	XM_024452260.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitochondrial tRNA-specific 2-thiouridylase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAACT GGGACTCACT GGATGAACAT GGGGTCTGTA CTGCCGACAA AGACTGTGAA 
GATGCTTACA GAGTTTGCCA GATCTTAGAC ATCCCTTTCC ATCAAGTGTC CTACGTAAAG 
GAGTATTGGA ATGATGTGTT CAGTGACTTT TTGAATGAGT ATGAAAAAGG AAGGACTCCC 
AATCCTGACA TAGTTTGCAA CAAGCACATC AAATTTAGTT GCTTTTTTCA TTATGCTGTG 
GATAATCTTG GGGCAGATGC CATTGCCACA GGTCACTATG CAAGAACTTC CCTGGAAGAT 
GAAGAAGTCT TTGAGCAGAA GCACGTTAAG AAGCCCGAAG GGCTTTTCAG AAATCGGTTT 
GAAGTTAGAA ATGCGGTAAA ACTCCTCCAG GCAGCTGACA GCTTTAAAGA CCAGACCTTC 
TTTCTCAGCC AGGTTTCCCA GGATGCCCTG AGGAGAACCA TCTTCCCTCT GGGGGGATTA 
ACGAAAGAGT TTGTAAAGAA AATCGCTGCT GAGAATAGAC TTCATCATGT GCTTCAGAAG 
AAAGAGAGCA TGGGCATGTG TTTCATCGGG AAGAGGAATT TTGAACATTT CCTTCTTCAG 
TATCTGCAGC CTCGACCTGG TCACTTTATT TCCATAGAAG ACAATAAGGT TCTGGGAACA 
CATAAAGGCC CCCCGGACAG ACCACCCAGC CCTGTACAGG GACCTGCTGA GGACCAGCCG 
CGTGCACTGG ATTGCGGAGG AGCCTCCCGC AGCACTGGTC CGGGACAAGA TGATGGAGTG 
CCACTTCCGA TTCCGCCACC AGATGGCACT AGTGCCCTGT GTGCTGACCC TCAATCAAGA 
TGGCACCGTG TGGGTGACAG CTGTGCAGGC TGTGCGTGCC CTTGCCACAG GACAGTTTGC 
TGTGTTCTAC AAGGGGGACG AGTGCCTGGG CAGCGGGAAG ATCCTGCGGC TGGGGCCGTC 
TGCCTACACG CTCCAGAAGG GCCAGCGCAG AGCTGGGATG GCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308028.1
CDS	188..1195
Translation	MKNWDSLDEHGVCTADKDCEDAYRVCQILDIPFHQVSYVKEYWNDVFSDFLNEYEKGRTPNPDIVCNKHIKFSCFFHYAVDNLGADAIATGHYARTSLEDEEVFEQKHVKKPEGLFRNRFEVRNAVKLLQAADSFKDQTFFLSQVSQDALRRTIFPLGGLTKEFVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHFISIEDNKVLGTHKGPPDRPPSPVQGPAEDQPRALDCGGASRSTGPGQDDGVPLPIPPPDGTSALCADPQSRWHRVGDSCAGCACPCHRTVCCVLQGGRVPGQREDPAAGAVCLHAPEGPAQSWDGH

Target ORF information:

RefSeq Version	XM_024452260.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452260.1

ORF Insert Sequence:

ATGAAGAACT GGGACTCACT GGATGAACAT GGGGTCTGTA CTGCCGACAA AGACTGTGAA 
GATGCTTACA GAGTTTGCCA GATCTTAGAC ATCCCTTTCC ATCAAGTGTC CTACGTAAAG 
GAGTATTGGA ATGATGTGTT CAGTGACTTT TTGAATGAGT ATGAAAAAGG AAGGACTCCC 
AATCCTGACA TAGTTTGCAA CAAGCACATC AAATTTAGTT GCTTTTTTCA TTATGCTGTG 
GATAATCTTG GGGCAGATGC CATTGCCACA GGTCACTATG CAAGAACTTC CCTGGAAGAT 
GAAGAAGTCT TTGAGCAGAA GCACGTTAAG AAGCCCGAAG GGCTTTTCAG AAATCGGTTT 
GAAGTTAGAA ATGCGGTAAA ACTCCTCCAG GCAGCTGACA GCTTTAAAGA CCAGACCTTC 
TTTCTCAGCC AGGTTTCCCA GGATGCCCTG AGGAGAACCA TCTTCCCTCT GGGGGGATTA 
ACGAAAGAGT TTGTAAAGAA AATCGCTGCT GAGAATAGAC TTCATCATGT GCTTCAGAAG 
AAAGAGAGCA TGGGCATGTG TTTCATCGGG AAGAGGAATT TTGAACATTT CCTTCTTCAG 
TATCTGCAGC CTCGACCTGG TCACTTTATT TCCATAGAAG ACAATAAGGT TCTGGGAACA 
CATAAAGGCC CCCCGGACAG ACCACCCAGC CCTGTACAGG GACCTGCTGA GGACCAGCCG 
CGTGCACTGG ATTGCGGAGG AGCCTCCCGC AGCACTGGTC CGGGACAAGA TGATGGAGTG 
CCACTTCCGA TTCCGCCACC AGATGGCACT AGTGCCCTGT GTGCTGACCC TCAATCAAGA 
TGGCACCGTG TGGGTGACAG CTGTGCAGGC TGTGCGTGCC CTTGCCACAG GACAGTTTGC 
TGTGTTCTAC AAGGGGGACG AGTGCCTGGG CAGCGGGAAG ATCCTGCGGC TGGGGCCGTC 
TGCCTACACG CTCCAGAAGG GCCAGCGCAG AGCTGGGATG GCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Biochemical Evidence for a Nuclear Modifier Allele (A10S) in TRMU (Methylaminomethyl-2-thiouridylate-methyltransferase) Related to Mitochondrial tRNA Modification in the Phenotypic Manifestation of Deafness-associated 12S rRNA Mutation.
The Journal of biological chemistry292(7)2881-2892(2017 02)
Meng F,Cang X,Peng Y,Li R,Zhang Z,Li F,Fan Q,Guan AS,Fischel-Ghosian N,Zhao X,Guan MX

The ROS-sensitive microRNA-9/9* controls the expression of mitochondrial tRNA-modifying enzymes and is involved in the molecular mechanism of MELAS syndrome.
Human molecular genetics24(1)167-84(2015 Jan)
Meseguer S,Mart?nez-Zamora A,Garc?a-Arum? E,Andreu AL,Armengod ME

Nuclear factors: roles related to mitochondrial deafness.
Gene520(2)79-89(2013 May)
Luo LF,Hou CC,Yang WX

A novel mutation in YARS2 causes myopathy with lactic acidosis and sideroblastic anemia.
Human mutation33(8)1201-6(2012 Aug)
Sasarman F,Nishimura T,Thiffault I,Shoubridge EA

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TRMU cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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FAX

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