SCYL2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SCYL2
Entrez Gene ID	55681
Full Name	SCY1 like pseudokinase 2
Synonyms	CVAK104
General protein information	Preferred Names SCY1 like pseudokinase 2 Names SCY1-like protein 2 SCY1-like 2 SCY1-like, kinase-like 2 coated vesicle-associated kinase of 104 kDa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q23.1
Summary	The protein encoded by this gene associates with clathrin-coated complexes at the plasma membrane and with endocytic coated vesicles. The encoded protein phosphorylates the beta2 subunit of the plasma membrane adapter complex AP2 and interacts with clathrin, showing involvement in clathrin-dependent pathways between the trans-Golgi network and the endosomal system. In addition, this protein has a role in the Wnt signaling pathway by targeting frizzled 5 (Fzd5) for lysosomal degradation. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2015].
Disorder MIM:	616365

mRNA and Protein(s)

mRNA	Protein	Name
NM_017988.5	NP_060458.3	SCY1-like protein 2 isoform 1
NM_001317784.1	NP_001304713.1	SCY1-like protein 2 isoform 1
NM_001330254.1	NP_001317183.1	SCY1-like protein 2 isoform 2
NM_001330253.1	NP_001317182.1	SCY1-like protein 2 isoform 2
NM_017988.6	NP_060458.3	SCY1-like protein 2 isoform 1
NM_001330256.1	NP_001317185.1	SCY1-like protein 2 isoform 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	SCYL2	NP_060458.3
Danio rerio (zebrafish)	si:ch211-244b2.1	NP_001074268.1
Mus musculus (house mouse)	Scyl2	NP_932138.1
Gallus gallus (chicken)	SCYL2	NP_001025922.1
Drosophila melanogaster (fruit fly)	CG1951	NP_651655.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002142	XP_308053.4
Bos taurus (cattle)	SCYL2	NP_001095999.1
Canis lupus familiaris (dog)	SCYL2	XP_005629328.1
Xenopus tropicalis (tropical clawed frog)	scyl2	XP_002936877.2
Rattus norvegicus (Norway rat)	Scyl2	NP_001178709.1

Gene Ontology
Bibliography

Related articles in PubMed

Interferon-induced SCYL2 limits release of HIV-1 by triggering PP2A-mediated dephosphorylation of the viral protein Vpu.
Miyakawa K, Sawasaki T, Matsunaga S, Tokarev A, Quinn G, Kimura H, Nomaguchi M, Adachi A, Yamamoto N, Guatelli J, Ryo A
Science signaling5(245)ra73(2012 Oct)

A coated vesicle-associated kinase of 104 kDa (CVAK104) induces lysosomal degradation of frizzled 5 (Fzd5).
Terabayashi T, Funato Y, Fukuda M, Miki H
The Journal of biological chemistry284(39)26716-24(2009 Sep)

CVAK104 is a novel regulator of clathrin-mediated SNARE sorting.
Borner GH, Rana AA, Forster R, Harbour M, Smith JC, Robinson MS
Traffic (Copenhagen, Denmark)8(7)893-903(2007 Jul)

Clathrin-dependent association of CVAK104 with endosomes and the trans-Golgi network.
D?wel M, Ungewickell EJ
Molecular biology of the cell17(10)4513-25(2006 Oct)

Role of the AP2 beta-appendage hub in recruiting partners for clathrin-coated vesicle assembly.
Schmid EM, Ford MG, Burtey A, Praefcke GJ, Peak-Chew SY, Mills IG, Benmerah A, McMahon HT
PLoS biology4(9)e262(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results suggest that SCYL2 serves as a regulatory factor for Vpu, reducing the extent of Vpu phosphorylation and consequently enhancing BST2-mediated viral restriction.
Title: Interferon-induced SCYL2 limits release of HIV-1 by triggering PP2A-mediated dephosphorylation of the viral protein Vpu.

Studies identify CVAK104 as a novel binding partner of Dishevelled (Dvl) and that CVAK104 also interacts with Fzd5.
Title: A coated vesicle-associated kinase of 104 kDa (CVAK104) induces lysosomal degradation of frizzled 5 (Fzd5).

CVAK104 is an evolutionarily conserved protein involved in SNARE sorting that is essential for normal embryonic development
Title: CVAK104 is a novel regulator of clathrin-mediated SNARE sorting.

Together, our data suggest a function for CVAK104 in clathrin-dependent pathways between the trans-Golgi network and the endosomal system.
Title: Clathrin-dependent association of CVAK104 with endosomes and the trans-Golgi network.

CVAK104 binds ATP and functions in vitro as a poly-L-lysine-stimulated kinase that is capable of autophosphorylation and phosphorylating the beta2-adaptin subunit of AP2.
Title: CVAK104 is a novel poly-L-lysine-stimulated kinase that targets the beta2-subunit of AP2.

The following SCYL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SCYL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu29664	NM_017988.6 Latest version!	Homo sapiens SCY1 like pseudokinase 2 (SCYL2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu29664	NM_001317784.1 Latest version!	Homo sapiens SCY1 like pseudokinase 2 (SCYL2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu101275	NM_001330253.1 Latest version!	Homo sapiens SCY1 like pseudokinase 2 (SCYL2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu101275	NM_001330254.1 Latest version!	Homo sapiens SCY1 like pseudokinase 2 (SCYL2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu101276	NM_001330256.1 Latest version!	Homo sapiens SCY1 like pseudokinase 2 (SCYL2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu29664	NM_017988.5	Homo sapiens SCY1 like pseudokinase 2 (SCYL2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SCYL2 ( NM_017988.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060458.3 Homo sapiens SCY1 like pseudokinase 2 (SCYL2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu29664
Clone ID Related Accession (Same CDS sequence)	NM_017988.5 , NM_001317784.1 , NM_017988.6
Accession Version	NM_017988.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	SCY1-like protein 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB127407.1, BC063798.1, AB037781.1 and CN337316.1. On Dec 9, 2015 this sequence version replaced NM_017988.4. Transcript Variant: This variant (1) encodes isoform 1. Both variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.121856.1, SRR1660809.195646.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTCCA TGCTTAATAA ATTGAAGAGT ACTGTTACAA AAGTAACAGC TGATGTCACT 
AGTGCTGTAA TGGGAAATCC TGTCACTAGA GAATTTGATG TTGGTCGACA CATTGCCAGT 
GGTGGCAATG GGCTAGCTTG GAAGATTTTT AATGGCACAA AAAAGTCAAC AAAGCAGGAA 
GTGGCAGTTT TTGTCTTTGA TAAAAAACTG ATTGACAAGT ATCAAAAATT TGAAAAGGAT 
CAAATCATTG ATTCTCTAAA ACGAGGAGTC CAACAGTTAA CTCGGCTTCG ACACCCTCGA 
CTTCTTACTG TCCAGCATCC TTTAGAAGAA TCCAGGGATT GCTTGGCATT TTGTACAGAA 
CCAGTTTTTG CCAGTTTAGC CAATGTTCTT GGTAACTGGG AAAATCTACC TTCCCCTATA 
TCTCCAGACA TTAAGGATTA TAAACTTTAT GATGTAGAAA CCAAATATGG TTTGCTTCAG 
GTTTCTGAAG GATTGTCATT CTTGCATAGC AGTGTGAAAA TGGTGCATGG AAATATCACT 
CCTGAAAATA TAATTTTGAA TAAAAGTGGA GCCTGGAAAA TAATGGGTTT TGATTTTTGT 
GTATCATCAA CCAATCCTTC TGAACAAGAG CCTAAATTTC CTTGTAAAGA ATGGGACCCA 
AATTTACCTT CATTGTGTCT TCCAAATCCT GAATATTTGG CTCCTGAATA CATACTTTCT 
GTGAGCTGTG AAACAGCCAG TGATATGTAT TCTTTAGGAA CTGTTATGTA TGCTGTATTT 
AATAAAGGGA AACCTATATT TGAAGTCAAC AAGCAAGATA TTTACAAGAG TTTCAGTAGG 
CAGTTGGATC AGTTGAGTCG TTTAGGATCT AGTTCACTTA CAAATATACC TGAGGAAGTT 
CGTGAACATG TAAAGCTACT GTTAAATGTA ACTCCGACTG TAAGACCAGA TGCAGATCAA 
ATGACAAAGA TTCCCTTCTT TGATGATGTT GGTGCAGTAA CACTGCAATA TTTTGATACC 
TTATTCCAAA GAGATAATCT TCAGAAATCA CAGTTTTTCA AAGGACTGCC AAAGGTTCTA 
CCAAAACTGC CCAAGCGTGT CATTGTGCAG AGAATTTTGC CTTGTTTGAC TTCAGAATTT 
GTAAACCCTG ACATGGTACC TTTTGTTTTG CCCAATGTTC TACTTATTGC TGAGGAATGC 
ACCAAAGAAG AATATGTCAA ATTAATTCTT CCTGAACTTG GCCCTGTGTT TAAGCAGCAG 
GAGCCAATCC AGATTTTGTT AATTTTCCTA CAAAAAATGG ATTTGCTACT AACCAAAACC 
CCTCCTGATG AGATAAAGAA CAGTGTTCTA CCCATGGTTT ACAGAGCACT AGAAGCTCCT 
TCCATTCAGA TCCAGGAGCT CTGTCTAAAC ATCATTCCAA CCTTTGCAAA TCTTATAGAC 
TACCCATCCA TGAAAAACGC TTTGATACCA AGAATTAAAA ATGCTTGTCT ACAAACATCT 
TCCCTTGCGG TTCGTGTAAA TTCATTAGTG TGCTTAGGAA AGATTTTGGA ATACTTGGAT 
AAGTGGTTTG TACTTGATGA TATCCTACCC TTCTTACAAC AAATTCCATC CAAGGAACCT 
GCGGTCCTCA TGGGAATTTT AGGTATTTAC AAATGTACTT TTACTCATAA GAAGTTGGGA 
ATCACCAAAG AGCAGCTGGC CGGAAAAGTG TTGCCTCATC TTATTCCCCT GAGTATTGAA 
AACAATCTTA ATCTTAATCA GTTCAATTCT TTCATTTCCG TCATAAAAGA AATGCTTAAT 
AGATTGGAGT CTGAACATAA GACTAAACTG GAGCAACTTC ATATAATGCA AGAACAGCAG 
AAATCTTTGG ATATAGGAAA TCAAATGAAT GTTTCTGAGG AGATGAAAGT TACAAATATT 
GGGAATCAGC AAATTGACAA AGTTTTTAAC AACATTGGAG CAGACCTTCT GACTGGCAGT 
GAGTCCGAAA ATAAAGAGGA CGGGTTACAG AATAAACATA AAAGAGCATC ACTTACACTT 
GAAGAAAAAC AAAAATTAGC AAAAGAACAA GAGCAGGCAC AGAAGCTGAA AAGCCAGCAG 
CCTCTTAAAC CCCAAGTGCA CACACCTGTT GCTACTGTTA AACAGACTAA GGACTTGACA 
GACACACTGA TGGATAATAT GTCATCCTTG ACCAGCCTTT CTGTTAGTAC CCCTAAATCT 
TCTGCTTCAA GTACTTTCAC TTCTGTTCCT TCCATGGGCA TTGGTATGAT GTTTTCTACA 
CCAACTGATA ATACAAAGAG AAATTTGACA AATGGCCTAA ATGCCAATAT GGGCTTTCAG 
ACTTCAGGAT TCAACATGCC CGTTAATACA AACCAGAACT TCTACAGTAG TCCAAGCACA 
GTTGGAGTGA CCAAGATGAC TCTGGGAACA CCTCCCACTT TGCCAAACTT CAATGCTTTG 
AGTGTTCCTC CTGCTGGTGC AAAGCAGACC CAACAAAGAC CCACAGATAT GTCTGCCCTT 
AATAATCTCT TTGGCCCTCA GAAACCCAAA GTTAGCATGA ACCAGTTATC ACAACAGAAA 
CCAAATCAGT GGCTTAATCA GTTTGTACCT CCTCAAGGTT CTCCAACTAT GGGCAGTTCA 
GTAATGGGGA CACAGATGAA CGTGATAGGA CAATCTGCTT TTGGTATGCA GGGTAATCCT 
TTCTTTAACC CACAGAACTT TGCACAGCCA CCAACTACTA TGACCAATAG CAGTTCAGCT 
AGCAATGATT TAAAAGATCT TTTTGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060458.3
CDS	438..3227
Translation	MESMLNKLKSTVTKVTADVTSAVMGNPVTREFDVGRHIASGGNGLAWKIFNGTKKSTKQEVAVFVFDKKLIDKYQKFEKDQIIDSLKRGVQQLTRLRHPRLLTVQHPLEESRDCLAFCTEPVFASLANVLGNWENLPSPISPDIKDYKLYDVETKYGLLQVSEGLSFLHSSVKMVHGNITPENIILNKSGAWKIMGFDFCVSSTNPSEQEPKFPCKEWDPNLPSLCLPNPEYLAPEYILSVSCETASDMYSLGTVMYAVFNKGKPIFEVNKQDIYKSFSRQLDQLSRLGSSSLTNIPEEVREHVKLLLNVTPTVRPDADQMTKIPFFDDVGAVTLQYFDTLFQRDNLQKSQFFKGLPKVLPKLPKRVIVQRILPCLTSEFVNPDMVPFVLPNVLLIAEECTKEEYVKLILPELGPVFKQQEPIQILLIFLQKMDLLLTKTPPDEIKNSVLPMVYRALEAPSIQIQELCLNIIPTFANLIDYPSMKNALIPRIKNACLQTSSLAVRVNSLVCLGKILEYLDKWFVLDDILPFLQQIPSKEPAVLMGILGIYKCTFTHKKLGITKEQLAGKVLPHLIPLSIENNLNLNQFNSFISVIKEMLNRLESEHKTKLEQLHIMQEQQKSLDIGNQMNVSEEMKVTNIGNQQIDKVFNNIGADLLTGSESENKEDGLQNKHKRASLTLEEKQKLAKEQEQAQKLKSQQPLKPQVHTPVATVKQTKDLTDTLMDNMSSLTSLSVSTPKSSASSTFTSVPSMGIGMMFSTPTDNTKRNLTNGLNANMGFQTSGFNMPVNTNQNFYSSPSTVGVTKMTLGTPPTLPNFNALSVPPAGAKQTQQRPTDMSALNNLFGPQKPKVSMNQLSQQKPNQWLNQFVPPQGSPTMGSSVMGTQMNVIGQSAFGMQGNPFFNPQNFAQPPTTMTNSSSASNDLKDLFG

Target ORF information:

RefSeq Version	NM_017988.5
Organism	Homo sapiens(human)
Definition	Homo sapiens SCY1 like pseudokinase 2 (SCYL2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017988.5

ORF Insert Sequence:

ATGGAGTCCA TGCTTAATAA ATTGAAGAGT ACTGTTACAA AAGTAACAGC TGATGTCACT 
AGTGCTGTAA TGGGAAATCC TGTCACTAGA GAATTTGATG TTGGTCGACA CATTGCCAGT 
GGTGGCAATG GGCTAGCTTG GAAGATTTTT AATGGCACAA AAAAGTCAAC AAAGCAGGAA 
GTGGCAGTTT TTGTCTTTGA TAAAAAACTG ATTGACAAGT ATCAAAAATT TGAAAAGGAT 
CAAATCATTG ATTCTCTAAA ACGAGGAGTC CAACAGTTAA CTCGGCTTCG ACACCCTCGA 
CTTCTTACTG TCCAGCATCC TTTAGAAGAA TCCAGGGATT GCTTGGCATT TTGTACAGAA 
CCAGTTTTTG CCAGTTTAGC CAATGTTCTT GGTAACTGGG AAAATCTACC TTCCCCTATA 
TCTCCAGACA TTAAGGATTA TAAACTTTAT GATGTAGAAA CCAAATATGG TTTGCTTCAG 
GTTTCTGAAG GATTGTCATT CTTGCATAGC AGTGTGAAAA TGGTGCATGG AAATATCACT 
CCTGAAAATA TAATTTTGAA TAAAAGTGGA GCCTGGAAAA TAATGGGTTT TGATTTTTGT 
GTATCATCAA CCAATCCTTC TGAACAAGAG CCTAAATTTC CTTGTAAAGA ATGGGACCCA 
AATTTACCTT CATTGTGTCT TCCAAATCCT GAATATTTGG CTCCTGAATA CATACTTTCT 
GTGAGCTGTG AAACAGCCAG TGATATGTAT TCTTTAGGAA CTGTTATGTA TGCTGTATTT 
AATAAAGGGA AACCTATATT TGAAGTCAAC AAGCAAGATA TTTACAAGAG TTTCAGTAGG 
CAGTTGGATC AGTTGAGTCG TTTAGGATCT AGTTCACTTA CAAATATACC TGAGGAAGTT 
CGTGAACATG TAAAGCTACT GTTAAATGTA ACTCCGACTG TAAGACCAGA TGCAGATCAA 
ATGACAAAGA TTCCCTTCTT TGATGATGTT GGTGCAGTAA CACTGCAATA TTTTGATACC 
TTATTCCAAA GAGATAATCT TCAGAAATCA CAGTTTTTCA AAGGACTGCC AAAGGTTCTA 
CCAAAACTGC CCAAGCGTGT CATTGTGCAG AGAATTTTGC CTTGTTTGAC TTCAGAATTT 
GTAAACCCTG ACATGGTACC TTTTGTTTTG CCCAATGTTC TACTTATTGC TGAGGAATGC 
ACCAAAGAAG AATATGTCAA ATTAATTCTT CCTGAACTTG GCCCTGTGTT TAAGCAGCAG 
GAGCCAATCC AGATTTTGTT AATTTTCCTA CAAAAAATGG ATTTGCTACT AACCAAAACC 
CCTCCTGATG AGATAAAGAA CAGTGTTCTA CCCATGGTTT ACAGAGCACT AGAAGCTCCT 
TCCATTCAGA TCCAGGAGCT CTGTCTAAAC ATCATTCCAA CCTTTGCAAA TCTTATAGAC 
TACCCATCCA TGAAAAACGC TTTGATACCA AGAATTAAAA ATGCTTGTCT ACAAACATCT 
TCCCTTGCGG TTCGTGTAAA TTCATTAGTG TGCTTAGGAA AGATTTTGGA ATACTTGGAT 
AAGTGGTTTG TACTTGATGA TATCCTACCC TTCTTACAAC AAATTCCATC CAAGGAACCT 
GCGGTCCTCA TGGGAATTTT AGGTATTTAC AAATGTACTT TTACTCATAA GAAGTTGGGA 
ATCACCAAAG AGCAGCTGGC CGGAAAAGTG TTGCCTCATC TTATTCCCCT GAGTATTGAA 
AACAATCTTA ATCTTAATCA GTTCAATTCT TTCATTTCCG TCATAAAAGA AATGCTTAAT 
AGATTGGAGT CTGAACATAA GACTAAACTG GAGCAACTTC ATATAATGCA AGAACAGCAG 
AAATCTTTGG ATATAGGAAA TCAAATGAAT GTTTCTGAGG AGATGAAAGT TACAAATATT 
GGGAATCAGC AAATTGACAA AGTTTTTAAC AACATTGGAG CAGACCTTCT GACTGGCAGT 
GAGTCCGAAA ATAAAGAGGA CGGGTTACAG AATAAACATA AAAGAGCATC ACTTACACTT 
GAAGAAAAAC AAAAATTAGC AAAAGAACAA GAGCAGGCAC AGAAGCTGAA AAGCCAGCAG 
CCTCTTAAAC CCCAAGTGCA CACACCTGTT GCTACTGTTA AACAGACTAA GGACTTGACA 
GACACACTGA TGGATAATAT GTCATCCTTG ACCAGCCTTT CTGTTAGTAC CCCTAAATCT 
TCTGCTTCAA GTACTTTCAC TTCTGTTCCT TCCATGGGCA TTGGTATGAT GTTTTCTACA 
CCAACTGATA ATACAAAGAG AAATTTGACA AATGGCCTAA ATGCCAATAT GGGCTTTCAG 
ACTTCAGGAT TCAACATGCC CGTTAATACA AACCAGAACT TCTACAGTAG TCCAAGCACA 
GTTGGAGTGA CCAAGATGAC TCTGGGAACA CCTCCCACTT TGCCAAACTT CAATGCTTTG 
AGTGTTCCTC CTGCTGGTGC AAAGCAGACC CAACAAAGAC CCACAGATAT GTCTGCCCTT 
AATAATCTCT TTGGCCCTCA GAAACCCAAA GTTAGCATGA ACCAGTTATC ACAACAGAAA 
CCAAATCAGT GGCTTAATCA GTTTGTACCT CCTCAAGGTT CTCCAACTAT GGGCAGTTCA 
GTAATGGGGA CACAGATGAA CGTGATAGGA CAATCTGCTT TTGGTATGCA GGGTAATCCT 
TTCTTTAACC CACAGAACTT TGCACAGCCA CCAACTACTA TGACCAATAG CAGTTCAGCT 
AGCAATGATT TAAAAGATCT TTTTGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCYL2 ( NM_001317784.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001304713.1 Homo sapiens SCY1 like pseudokinase 2 (SCYL2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu29664
Clone ID Related Accession (Same CDS sequence)	NM_017988.5 , NM_001317784.1 , NM_017988.6
Accession Version	NM_001317784.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	SCY1-like protein 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA234206.1, BC011525.1, AB037781.1 and CN337316.1. On Dec 9, 2015 this sequence version replaced XM_005269018.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660805.258510.1, SRR1660805.117939.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTCCA TGCTTAATAA ATTGAAGAGT ACTGTTACAA AAGTAACAGC TGATGTCACT 
AGTGCTGTAA TGGGAAATCC TGTCACTAGA GAATTTGATG TTGGTCGACA CATTGCCAGT 
GGTGGCAATG GGCTAGCTTG GAAGATTTTT AATGGCACAA AAAAGTCAAC AAAGCAGGAA 
GTGGCAGTTT TTGTCTTTGA TAAAAAACTG ATTGACAAGT ATCAAAAATT TGAAAAGGAT 
CAAATCATTG ATTCTCTAAA ACGAGGAGTC CAACAGTTAA CTCGGCTTCG ACACCCTCGA 
CTTCTTACTG TCCAGCATCC TTTAGAAGAA TCCAGGGATT GCTTGGCATT TTGTACAGAA 
CCAGTTTTTG CCAGTTTAGC CAATGTTCTT GGTAACTGGG AAAATCTACC TTCCCCTATA 
TCTCCAGACA TTAAGGATTA TAAACTTTAT GATGTAGAAA CCAAATATGG TTTGCTTCAG 
GTTTCTGAAG GATTGTCATT CTTGCATAGC AGTGTGAAAA TGGTGCATGG AAATATCACT 
CCTGAAAATA TAATTTTGAA TAAAAGTGGA GCCTGGAAAA TAATGGGTTT TGATTTTTGT 
GTATCATCAA CCAATCCTTC TGAACAAGAG CCTAAATTTC CTTGTAAAGA ATGGGACCCA 
AATTTACCTT CATTGTGTCT TCCAAATCCT GAATATTTGG CTCCTGAATA CATACTTTCT 
GTGAGCTGTG AAACAGCCAG TGATATGTAT TCTTTAGGAA CTGTTATGTA TGCTGTATTT 
AATAAAGGGA AACCTATATT TGAAGTCAAC AAGCAAGATA TTTACAAGAG TTTCAGTAGG 
CAGTTGGATC AGTTGAGTCG TTTAGGATCT AGTTCACTTA CAAATATACC TGAGGAAGTT 
CGTGAACATG TAAAGCTACT GTTAAATGTA ACTCCGACTG TAAGACCAGA TGCAGATCAA 
ATGACAAAGA TTCCCTTCTT TGATGATGTT GGTGCAGTAA CACTGCAATA TTTTGATACC 
TTATTCCAAA GAGATAATCT TCAGAAATCA CAGTTTTTCA AAGGACTGCC AAAGGTTCTA 
CCAAAACTGC CCAAGCGTGT CATTGTGCAG AGAATTTTGC CTTGTTTGAC TTCAGAATTT 
GTAAACCCTG ACATGGTACC TTTTGTTTTG CCCAATGTTC TACTTATTGC TGAGGAATGC 
ACCAAAGAAG AATATGTCAA ATTAATTCTT CCTGAACTTG GCCCTGTGTT TAAGCAGCAG 
GAGCCAATCC AGATTTTGTT AATTTTCCTA CAAAAAATGG ATTTGCTACT AACCAAAACC 
CCTCCTGATG AGATAAAGAA CAGTGTTCTA CCCATGGTTT ACAGAGCACT AGAAGCTCCT 
TCCATTCAGA TCCAGGAGCT CTGTCTAAAC ATCATTCCAA CCTTTGCAAA TCTTATAGAC 
TACCCATCCA TGAAAAACGC TTTGATACCA AGAATTAAAA ATGCTTGTCT ACAAACATCT 
TCCCTTGCGG TTCGTGTAAA TTCATTAGTG TGCTTAGGAA AGATTTTGGA ATACTTGGAT 
AAGTGGTTTG TACTTGATGA TATCCTACCC TTCTTACAAC AAATTCCATC CAAGGAACCT 
GCGGTCCTCA TGGGAATTTT AGGTATTTAC AAATGTACTT TTACTCATAA GAAGTTGGGA 
ATCACCAAAG AGCAGCTGGC CGGAAAAGTG TTGCCTCATC TTATTCCCCT GAGTATTGAA 
AACAATCTTA ATCTTAATCA GTTCAATTCT TTCATTTCCG TCATAAAAGA AATGCTTAAT 
AGATTGGAGT CTGAACATAA GACTAAACTG GAGCAACTTC ATATAATGCA AGAACAGCAG 
AAATCTTTGG ATATAGGAAA TCAAATGAAT GTTTCTGAGG AGATGAAAGT TACAAATATT 
GGGAATCAGC AAATTGACAA AGTTTTTAAC AACATTGGAG CAGACCTTCT GACTGGCAGT 
GAGTCCGAAA ATAAAGAGGA CGGGTTACAG AATAAACATA AAAGAGCATC ACTTACACTT 
GAAGAAAAAC AAAAATTAGC AAAAGAACAA GAGCAGGCAC AGAAGCTGAA AAGCCAGCAG 
CCTCTTAAAC CCCAAGTGCA CACACCTGTT GCTACTGTTA AACAGACTAA GGACTTGACA 
GACACACTGA TGGATAATAT GTCATCCTTG ACCAGCCTTT CTGTTAGTAC CCCTAAATCT 
TCTGCTTCAA GTACTTTCAC TTCTGTTCCT TCCATGGGCA TTGGTATGAT GTTTTCTACA 
CCAACTGATA ATACAAAGAG AAATTTGACA AATGGCCTAA ATGCCAATAT GGGCTTTCAG 
ACTTCAGGAT TCAACATGCC CGTTAATACA AACCAGAACT TCTACAGTAG TCCAAGCACA 
GTTGGAGTGA CCAAGATGAC TCTGGGAACA CCTCCCACTT TGCCAAACTT CAATGCTTTG 
AGTGTTCCTC CTGCTGGTGC AAAGCAGACC CAACAAAGAC CCACAGATAT GTCTGCCCTT 
AATAATCTCT TTGGCCCTCA GAAACCCAAA GTTAGCATGA ACCAGTTATC ACAACAGAAA 
CCAAATCAGT GGCTTAATCA GTTTGTACCT CCTCAAGGTT CTCCAACTAT GGGCAGTTCA 
GTAATGGGGA CACAGATGAA CGTGATAGGA CAATCTGCTT TTGGTATGCA GGGTAATCCT 
TTCTTTAACC CACAGAACTT TGCACAGCCA CCAACTACTA TGACCAATAG CAGTTCAGCT 
AGCAATGATT TAAAAGATCT TTTTGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304713.1
CDS	286..3075
Translation	MESMLNKLKSTVTKVTADVTSAVMGNPVTREFDVGRHIASGGNGLAWKIFNGTKKSTKQEVAVFVFDKKLIDKYQKFEKDQIIDSLKRGVQQLTRLRHPRLLTVQHPLEESRDCLAFCTEPVFASLANVLGNWENLPSPISPDIKDYKLYDVETKYGLLQVSEGLSFLHSSVKMVHGNITPENIILNKSGAWKIMGFDFCVSSTNPSEQEPKFPCKEWDPNLPSLCLPNPEYLAPEYILSVSCETASDMYSLGTVMYAVFNKGKPIFEVNKQDIYKSFSRQLDQLSRLGSSSLTNIPEEVREHVKLLLNVTPTVRPDADQMTKIPFFDDVGAVTLQYFDTLFQRDNLQKSQFFKGLPKVLPKLPKRVIVQRILPCLTSEFVNPDMVPFVLPNVLLIAEECTKEEYVKLILPELGPVFKQQEPIQILLIFLQKMDLLLTKTPPDEIKNSVLPMVYRALEAPSIQIQELCLNIIPTFANLIDYPSMKNALIPRIKNACLQTSSLAVRVNSLVCLGKILEYLDKWFVLDDILPFLQQIPSKEPAVLMGILGIYKCTFTHKKLGITKEQLAGKVLPHLIPLSIENNLNLNQFNSFISVIKEMLNRLESEHKTKLEQLHIMQEQQKSLDIGNQMNVSEEMKVTNIGNQQIDKVFNNIGADLLTGSESENKEDGLQNKHKRASLTLEEKQKLAKEQEQAQKLKSQQPLKPQVHTPVATVKQTKDLTDTLMDNMSSLTSLSVSTPKSSASSTFTSVPSMGIGMMFSTPTDNTKRNLTNGLNANMGFQTSGFNMPVNTNQNFYSSPSTVGVTKMTLGTPPTLPNFNALSVPPAGAKQTQQRPTDMSALNNLFGPQKPKVSMNQLSQQKPNQWLNQFVPPQGSPTMGSSVMGTQMNVIGQSAFGMQGNPFFNPQNFAQPPTTMTNSSSASNDLKDLFG

Target ORF information:

RefSeq Version	NM_001317784.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SCY1 like pseudokinase 2 (SCYL2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317784.1

ORF Insert Sequence:

ATGGAGTCCA TGCTTAATAA ATTGAAGAGT ACTGTTACAA AAGTAACAGC TGATGTCACT 
AGTGCTGTAA TGGGAAATCC TGTCACTAGA GAATTTGATG TTGGTCGACA CATTGCCAGT 
GGTGGCAATG GGCTAGCTTG GAAGATTTTT AATGGCACAA AAAAGTCAAC AAAGCAGGAA 
GTGGCAGTTT TTGTCTTTGA TAAAAAACTG ATTGACAAGT ATCAAAAATT TGAAAAGGAT 
CAAATCATTG ATTCTCTAAA ACGAGGAGTC CAACAGTTAA CTCGGCTTCG ACACCCTCGA 
CTTCTTACTG TCCAGCATCC TTTAGAAGAA TCCAGGGATT GCTTGGCATT TTGTACAGAA 
CCAGTTTTTG CCAGTTTAGC CAATGTTCTT GGTAACTGGG AAAATCTACC TTCCCCTATA 
TCTCCAGACA TTAAGGATTA TAAACTTTAT GATGTAGAAA CCAAATATGG TTTGCTTCAG 
GTTTCTGAAG GATTGTCATT CTTGCATAGC AGTGTGAAAA TGGTGCATGG AAATATCACT 
CCTGAAAATA TAATTTTGAA TAAAAGTGGA GCCTGGAAAA TAATGGGTTT TGATTTTTGT 
GTATCATCAA CCAATCCTTC TGAACAAGAG CCTAAATTTC CTTGTAAAGA ATGGGACCCA 
AATTTACCTT CATTGTGTCT TCCAAATCCT GAATATTTGG CTCCTGAATA CATACTTTCT 
GTGAGCTGTG AAACAGCCAG TGATATGTAT TCTTTAGGAA CTGTTATGTA TGCTGTATTT 
AATAAAGGGA AACCTATATT TGAAGTCAAC AAGCAAGATA TTTACAAGAG TTTCAGTAGG 
CAGTTGGATC AGTTGAGTCG TTTAGGATCT AGTTCACTTA CAAATATACC TGAGGAAGTT 
CGTGAACATG TAAAGCTACT GTTAAATGTA ACTCCGACTG TAAGACCAGA TGCAGATCAA 
ATGACAAAGA TTCCCTTCTT TGATGATGTT GGTGCAGTAA CACTGCAATA TTTTGATACC 
TTATTCCAAA GAGATAATCT TCAGAAATCA CAGTTTTTCA AAGGACTGCC AAAGGTTCTA 
CCAAAACTGC CCAAGCGTGT CATTGTGCAG AGAATTTTGC CTTGTTTGAC TTCAGAATTT 
GTAAACCCTG ACATGGTACC TTTTGTTTTG CCCAATGTTC TACTTATTGC TGAGGAATGC 
ACCAAAGAAG AATATGTCAA ATTAATTCTT CCTGAACTTG GCCCTGTGTT TAAGCAGCAG 
GAGCCAATCC AGATTTTGTT AATTTTCCTA CAAAAAATGG ATTTGCTACT AACCAAAACC 
CCTCCTGATG AGATAAAGAA CAGTGTTCTA CCCATGGTTT ACAGAGCACT AGAAGCTCCT 
TCCATTCAGA TCCAGGAGCT CTGTCTAAAC ATCATTCCAA CCTTTGCAAA TCTTATAGAC 
TACCCATCCA TGAAAAACGC TTTGATACCA AGAATTAAAA ATGCTTGTCT ACAAACATCT 
TCCCTTGCGG TTCGTGTAAA TTCATTAGTG TGCTTAGGAA AGATTTTGGA ATACTTGGAT 
AAGTGGTTTG TACTTGATGA TATCCTACCC TTCTTACAAC AAATTCCATC CAAGGAACCT 
GCGGTCCTCA TGGGAATTTT AGGTATTTAC AAATGTACTT TTACTCATAA GAAGTTGGGA 
ATCACCAAAG AGCAGCTGGC CGGAAAAGTG TTGCCTCATC TTATTCCCCT GAGTATTGAA 
AACAATCTTA ATCTTAATCA GTTCAATTCT TTCATTTCCG TCATAAAAGA AATGCTTAAT 
AGATTGGAGT CTGAACATAA GACTAAACTG GAGCAACTTC ATATAATGCA AGAACAGCAG 
AAATCTTTGG ATATAGGAAA TCAAATGAAT GTTTCTGAGG AGATGAAAGT TACAAATATT 
GGGAATCAGC AAATTGACAA AGTTTTTAAC AACATTGGAG CAGACCTTCT GACTGGCAGT 
GAGTCCGAAA ATAAAGAGGA CGGGTTACAG AATAAACATA AAAGAGCATC ACTTACACTT 
GAAGAAAAAC AAAAATTAGC AAAAGAACAA GAGCAGGCAC AGAAGCTGAA AAGCCAGCAG 
CCTCTTAAAC CCCAAGTGCA CACACCTGTT GCTACTGTTA AACAGACTAA GGACTTGACA 
GACACACTGA TGGATAATAT GTCATCCTTG ACCAGCCTTT CTGTTAGTAC CCCTAAATCT 
TCTGCTTCAA GTACTTTCAC TTCTGTTCCT TCCATGGGCA TTGGTATGAT GTTTTCTACA 
CCAACTGATA ATACAAAGAG AAATTTGACA AATGGCCTAA ATGCCAATAT GGGCTTTCAG 
ACTTCAGGAT TCAACATGCC CGTTAATACA AACCAGAACT TCTACAGTAG TCCAAGCACA 
GTTGGAGTGA CCAAGATGAC TCTGGGAACA CCTCCCACTT TGCCAAACTT CAATGCTTTG 
AGTGTTCCTC CTGCTGGTGC AAAGCAGACC CAACAAAGAC CCACAGATAT GTCTGCCCTT 
AATAATCTCT TTGGCCCTCA GAAACCCAAA GTTAGCATGA ACCAGTTATC ACAACAGAAA 
CCAAATCAGT GGCTTAATCA GTTTGTACCT CCTCAAGGTT CTCCAACTAT GGGCAGTTCA 
GTAATGGGGA CACAGATGAA CGTGATAGGA CAATCTGCTT TTGGTATGCA GGGTAATCCT 
TTCTTTAACC CACAGAACTT TGCACAGCCA CCAACTACTA TGACCAATAG CAGTTCAGCT 
AGCAATGATT TAAAAGATCT TTTTGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCYL2 ( NM_017988.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_060458.3 Homo sapiens SCY1 like pseudokinase 2 (SCYL2), transcript variant 1, mRNA.

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CloneID	OHu29664
Clone ID Related Accession (Same CDS sequence)	NM_017988.5 , NM_001317784.1 , NM_017988.6
Accession Version	NM_017988.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Homo sapiens(human)
Product	SCY1-like protein 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026110.42, DB127407.1, BC063798.1, AB037781.1 and CN337316.1. On May 17, 2019 this sequence version replaced NM_017988.5. Transcript Variant: This variant (1) encodes isoform 1. Both variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.121856.1, SRR1660809.195646.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000360820.7/ ENSP00000354061.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGTCCA TGCTTAATAA ATTGAAGAGT ACTGTTACAA AAGTAACAGC TGATGTCACT 
AGTGCTGTAA TGGGAAATCC TGTCACTAGA GAATTTGATG TTGGTCGACA CATTGCCAGT 
GGTGGCAATG GGCTAGCTTG GAAGATTTTT AATGGCACAA AAAAGTCAAC AAAGCAGGAA 
GTGGCAGTTT TTGTCTTTGA TAAAAAACTG ATTGACAAGT ATCAAAAATT TGAAAAGGAT 
CAAATCATTG ATTCTCTAAA ACGAGGAGTC CAACAGTTAA CTCGGCTTCG ACACCCTCGA 
CTTCTTACTG TCCAGCATCC TTTAGAAGAA TCCAGGGATT GCTTGGCATT TTGTACAGAA 
CCAGTTTTTG CCAGTTTAGC CAATGTTCTT GGTAACTGGG AAAATCTACC TTCCCCTATA 
TCTCCAGACA TTAAGGATTA TAAACTTTAT GATGTAGAAA CCAAATATGG TTTGCTTCAG 
GTTTCTGAAG GATTGTCATT CTTGCATAGC AGTGTGAAAA TGGTGCATGG AAATATCACT 
CCTGAAAATA TAATTTTGAA TAAAAGTGGA GCCTGGAAAA TAATGGGTTT TGATTTTTGT 
GTATCATCAA CCAATCCTTC TGAACAAGAG CCTAAATTTC CTTGTAAAGA ATGGGACCCA 
AATTTACCTT CATTGTGTCT TCCAAATCCT GAATATTTGG CTCCTGAATA CATACTTTCT 
GTGAGCTGTG AAACAGCCAG TGATATGTAT TCTTTAGGAA CTGTTATGTA TGCTGTATTT 
AATAAAGGGA AACCTATATT TGAAGTCAAC AAGCAAGATA TTTACAAGAG TTTCAGTAGG 
CAGTTGGATC AGTTGAGTCG TTTAGGATCT AGTTCACTTA CAAATATACC TGAGGAAGTT 
CGTGAACATG TAAAGCTACT GTTAAATGTA ACTCCGACTG TAAGACCAGA TGCAGATCAA 
ATGACAAAGA TTCCCTTCTT TGATGATGTT GGTGCAGTAA CACTGCAATA TTTTGATACC 
TTATTCCAAA GAGATAATCT TCAGAAATCA CAGTTTTTCA AAGGACTGCC AAAGGTTCTA 
CCAAAACTGC CCAAGCGTGT CATTGTGCAG AGAATTTTGC CTTGTTTGAC TTCAGAATTT 
GTAAACCCTG ACATGGTACC TTTTGTTTTG CCCAATGTTC TACTTATTGC TGAGGAATGC 
ACCAAAGAAG AATATGTCAA ATTAATTCTT CCTGAACTTG GCCCTGTGTT TAAGCAGCAG 
GAGCCAATCC AGATTTTGTT AATTTTCCTA CAAAAAATGG ATTTGCTACT AACCAAAACC 
CCTCCTGATG AGATAAAGAA CAGTGTTCTA CCCATGGTTT ACAGAGCACT AGAAGCTCCT 
TCCATTCAGA TCCAGGAGCT CTGTCTAAAC ATCATTCCAA CCTTTGCAAA TCTTATAGAC 
TACCCATCCA TGAAAAACGC TTTGATACCA AGAATTAAAA ATGCTTGTCT ACAAACATCT 
TCCCTTGCGG TTCGTGTAAA TTCATTAGTG TGCTTAGGAA AGATTTTGGA ATACTTGGAT 
AAGTGGTTTG TACTTGATGA TATCCTACCC TTCTTACAAC AAATTCCATC CAAGGAACCT 
GCGGTCCTCA TGGGAATTTT AGGTATTTAC AAATGTACTT TTACTCATAA GAAGTTGGGA 
ATCACCAAAG AGCAGCTGGC CGGAAAAGTG TTGCCTCATC TTATTCCCCT GAGTATTGAA 
AACAATCTTA ATCTTAATCA GTTCAATTCT TTCATTTCCG TCATAAAAGA AATGCTTAAT 
AGATTGGAGT CTGAACATAA GACTAAACTG GAGCAACTTC ATATAATGCA AGAACAGCAG 
AAATCTTTGG ATATAGGAAA TCAAATGAAT GTTTCTGAGG AGATGAAAGT TACAAATATT 
GGGAATCAGC AAATTGACAA AGTTTTTAAC AACATTGGAG CAGACCTTCT GACTGGCAGT 
GAGTCCGAAA ATAAAGAGGA CGGGTTACAG AATAAACATA AAAGAGCATC ACTTACACTT 
GAAGAAAAAC AAAAATTAGC AAAAGAACAA GAGCAGGCAC AGAAGCTGAA AAGCCAGCAG 
CCTCTTAAAC CCCAAGTGCA CACACCTGTT GCTACTGTTA AACAGACTAA GGACTTGACA 
GACACACTGA TGGATAATAT GTCATCCTTG ACCAGCCTTT CTGTTAGTAC CCCTAAATCT 
TCTGCTTCAA GTACTTTCAC TTCTGTTCCT TCCATGGGCA TTGGTATGAT GTTTTCTACA 
CCAACTGATA ATACAAAGAG AAATTTGACA AATGGCCTAA ATGCCAATAT GGGCTTTCAG 
ACTTCAGGAT TCAACATGCC CGTTAATACA AACCAGAACT TCTACAGTAG TCCAAGCACA 
GTTGGAGTGA CCAAGATGAC TCTGGGAACA CCTCCCACTT TGCCAAACTT CAATGCTTTG 
AGTGTTCCTC CTGCTGGTGC AAAGCAGACC CAACAAAGAC CCACAGATAT GTCTGCCCTT 
AATAATCTCT TTGGCCCTCA GAAACCCAAA GTTAGCATGA ACCAGTTATC ACAACAGAAA 
CCAAATCAGT GGCTTAATCA GTTTGTACCT CCTCAAGGTT CTCCAACTAT GGGCAGTTCA 
GTAATGGGGA CACAGATGAA CGTGATAGGA CAATCTGCTT TTGGTATGCA GGGTAATCCT 
TTCTTTAACC CACAGAACTT TGCACAGCCA CCAACTACTA TGACCAATAG CAGTTCAGCT 
AGCAATGATT TAAAAGATCT TTTTGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060458.3
CDS	645..3434
Translation	MESMLNKLKSTVTKVTADVTSAVMGNPVTREFDVGRHIASGGNGLAWKIFNGTKKSTKQEVAVFVFDKKLIDKYQKFEKDQIIDSLKRGVQQLTRLRHPRLLTVQHPLEESRDCLAFCTEPVFASLANVLGNWENLPSPISPDIKDYKLYDVETKYGLLQVSEGLSFLHSSVKMVHGNITPENIILNKSGAWKIMGFDFCVSSTNPSEQEPKFPCKEWDPNLPSLCLPNPEYLAPEYILSVSCETASDMYSLGTVMYAVFNKGKPIFEVNKQDIYKSFSRQLDQLSRLGSSSLTNIPEEVREHVKLLLNVTPTVRPDADQMTKIPFFDDVGAVTLQYFDTLFQRDNLQKSQFFKGLPKVLPKLPKRVIVQRILPCLTSEFVNPDMVPFVLPNVLLIAEECTKEEYVKLILPELGPVFKQQEPIQILLIFLQKMDLLLTKTPPDEIKNSVLPMVYRALEAPSIQIQELCLNIIPTFANLIDYPSMKNALIPRIKNACLQTSSLAVRVNSLVCLGKILEYLDKWFVLDDILPFLQQIPSKEPAVLMGILGIYKCTFTHKKLGITKEQLAGKVLPHLIPLSIENNLNLNQFNSFISVIKEMLNRLESEHKTKLEQLHIMQEQQKSLDIGNQMNVSEEMKVTNIGNQQIDKVFNNIGADLLTGSESENKEDGLQNKHKRASLTLEEKQKLAKEQEQAQKLKSQQPLKPQVHTPVATVKQTKDLTDTLMDNMSSLTSLSVSTPKSSASSTFTSVPSMGIGMMFSTPTDNTKRNLTNGLNANMGFQTSGFNMPVNTNQNFYSSPSTVGVTKMTLGTPPTLPNFNALSVPPAGAKQTQQRPTDMSALNNLFGPQKPKVSMNQLSQQKPNQWLNQFVPPQGSPTMGSSVMGTQMNVIGQSAFGMQGNPFFNPQNFAQPPTTMTNSSSASNDLKDLFG

Target ORF information:

RefSeq Version	NM_017988.6
Organism	Homo sapiens(human)
Definition	Homo sapiens SCY1 like pseudokinase 2 (SCYL2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017988.6

ORF Insert Sequence:

ATGGAGTCCA TGCTTAATAA ATTGAAGAGT ACTGTTACAA AAGTAACAGC TGATGTCACT 
AGTGCTGTAA TGGGAAATCC TGTCACTAGA GAATTTGATG TTGGTCGACA CATTGCCAGT 
GGTGGCAATG GGCTAGCTTG GAAGATTTTT AATGGCACAA AAAAGTCAAC AAAGCAGGAA 
GTGGCAGTTT TTGTCTTTGA TAAAAAACTG ATTGACAAGT ATCAAAAATT TGAAAAGGAT 
CAAATCATTG ATTCTCTAAA ACGAGGAGTC CAACAGTTAA CTCGGCTTCG ACACCCTCGA 
CTTCTTACTG TCCAGCATCC TTTAGAAGAA TCCAGGGATT GCTTGGCATT TTGTACAGAA 
CCAGTTTTTG CCAGTTTAGC CAATGTTCTT GGTAACTGGG AAAATCTACC TTCCCCTATA 
TCTCCAGACA TTAAGGATTA TAAACTTTAT GATGTAGAAA CCAAATATGG TTTGCTTCAG 
GTTTCTGAAG GATTGTCATT CTTGCATAGC AGTGTGAAAA TGGTGCATGG AAATATCACT 
CCTGAAAATA TAATTTTGAA TAAAAGTGGA GCCTGGAAAA TAATGGGTTT TGATTTTTGT 
GTATCATCAA CCAATCCTTC TGAACAAGAG CCTAAATTTC CTTGTAAAGA ATGGGACCCA 
AATTTACCTT CATTGTGTCT TCCAAATCCT GAATATTTGG CTCCTGAATA CATACTTTCT 
GTGAGCTGTG AAACAGCCAG TGATATGTAT TCTTTAGGAA CTGTTATGTA TGCTGTATTT 
AATAAAGGGA AACCTATATT TGAAGTCAAC AAGCAAGATA TTTACAAGAG TTTCAGTAGG 
CAGTTGGATC AGTTGAGTCG TTTAGGATCT AGTTCACTTA CAAATATACC TGAGGAAGTT 
CGTGAACATG TAAAGCTACT GTTAAATGTA ACTCCGACTG TAAGACCAGA TGCAGATCAA 
ATGACAAAGA TTCCCTTCTT TGATGATGTT GGTGCAGTAA CACTGCAATA TTTTGATACC 
TTATTCCAAA GAGATAATCT TCAGAAATCA CAGTTTTTCA AAGGACTGCC AAAGGTTCTA 
CCAAAACTGC CCAAGCGTGT CATTGTGCAG AGAATTTTGC CTTGTTTGAC TTCAGAATTT 
GTAAACCCTG ACATGGTACC TTTTGTTTTG CCCAATGTTC TACTTATTGC TGAGGAATGC 
ACCAAAGAAG AATATGTCAA ATTAATTCTT CCTGAACTTG GCCCTGTGTT TAAGCAGCAG 
GAGCCAATCC AGATTTTGTT AATTTTCCTA CAAAAAATGG ATTTGCTACT AACCAAAACC 
CCTCCTGATG AGATAAAGAA CAGTGTTCTA CCCATGGTTT ACAGAGCACT AGAAGCTCCT 
TCCATTCAGA TCCAGGAGCT CTGTCTAAAC ATCATTCCAA CCTTTGCAAA TCTTATAGAC 
TACCCATCCA TGAAAAACGC TTTGATACCA AGAATTAAAA ATGCTTGTCT ACAAACATCT 
TCCCTTGCGG TTCGTGTAAA TTCATTAGTG TGCTTAGGAA AGATTTTGGA ATACTTGGAT 
AAGTGGTTTG TACTTGATGA TATCCTACCC TTCTTACAAC AAATTCCATC CAAGGAACCT 
GCGGTCCTCA TGGGAATTTT AGGTATTTAC AAATGTACTT TTACTCATAA GAAGTTGGGA 
ATCACCAAAG AGCAGCTGGC CGGAAAAGTG TTGCCTCATC TTATTCCCCT GAGTATTGAA 
AACAATCTTA ATCTTAATCA GTTCAATTCT TTCATTTCCG TCATAAAAGA AATGCTTAAT 
AGATTGGAGT CTGAACATAA GACTAAACTG GAGCAACTTC ATATAATGCA AGAACAGCAG 
AAATCTTTGG ATATAGGAAA TCAAATGAAT GTTTCTGAGG AGATGAAAGT TACAAATATT 
GGGAATCAGC AAATTGACAA AGTTTTTAAC AACATTGGAG CAGACCTTCT GACTGGCAGT 
GAGTCCGAAA ATAAAGAGGA CGGGTTACAG AATAAACATA AAAGAGCATC ACTTACACTT 
GAAGAAAAAC AAAAATTAGC AAAAGAACAA GAGCAGGCAC AGAAGCTGAA AAGCCAGCAG 
CCTCTTAAAC CCCAAGTGCA CACACCTGTT GCTACTGTTA AACAGACTAA GGACTTGACA 
GACACACTGA TGGATAATAT GTCATCCTTG ACCAGCCTTT CTGTTAGTAC CCCTAAATCT 
TCTGCTTCAA GTACTTTCAC TTCTGTTCCT TCCATGGGCA TTGGTATGAT GTTTTCTACA 
CCAACTGATA ATACAAAGAG AAATTTGACA AATGGCCTAA ATGCCAATAT GGGCTTTCAG 
ACTTCAGGAT TCAACATGCC CGTTAATACA AACCAGAACT TCTACAGTAG TCCAAGCACA 
GTTGGAGTGA CCAAGATGAC TCTGGGAACA CCTCCCACTT TGCCAAACTT CAATGCTTTG 
AGTGTTCCTC CTGCTGGTGC AAAGCAGACC CAACAAAGAC CCACAGATAT GTCTGCCCTT 
AATAATCTCT TTGGCCCTCA GAAACCCAAA GTTAGCATGA ACCAGTTATC ACAACAGAAA 
CCAAATCAGT GGCTTAATCA GTTTGTACCT CCTCAAGGTT CTCCAACTAT GGGCAGTTCA 
GTAATGGGGA CACAGATGAA CGTGATAGGA CAATCTGCTT TTGGTATGCA GGGTAATCCT 
TTCTTTAACC CACAGAACTT TGCACAGCCA CCAACTACTA TGACCAATAG CAGTTCAGCT 
AGCAATGATT TAAAAGATCT TTTTGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCYL2 ( NM_001330254.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317183.1 Homo sapiens SCY1 like pseudokinase 2 (SCYL2), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu101275
Clone ID Related Accession (Same CDS sequence)	NM_001330254.1 , NM_001330253.1
Accession Version	NM_001330254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	SCY1-like protein 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA234206.1, BC011525.1, AB037781.1 and CN337316.1. On Aug 20, 2016 this sequence version replaced XM_017019618.1. Transcript Variant: This variant (4) differs in the 5' UTR and contains an alternate in-frame exon in its central coding region, compared to variant 1. The encoded isoform (2) has the same N- and C-termini, but is longer than isoform 1. Both variants 3 and 4 encode the same isoform (2). ##Evidence-Data-START## Transcript exon combination :: SRR1660807.192874.1, SRR1660809.169542.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTCCA TGCTTAATAA ATTGAAGAGT ACTGTTACAA AAGTAACAGC TGATGTCACT 
AGTGCTGTAA TGGGAAATCC TGTCACTAGA GAATTTGATG TTGGTCGACA CATTGCCAGT 
GGTGGCAATG GGCTAGCTTG GAAGATTTTT AATGGCACAA AAAAGTCAAC AAAGCAGGAA 
GTGGCAGTTT TTGTCTTTGA TAAAAAACTG ATTGACAAGT ATCAAAAATT TGAAAAGGAT 
CAAATCATTG ATTCTCTAAA ACGAGGAGTC CAACAGTTAA CTCGGCTTCG ACACCCTCGA 
CTTCTTACTG TCCAGCATCC TTTAGAAGAA TCCAGGGATT GCTTGGCATT TTGTACAGAA 
CCAGTTTTTG CCAGTTTAGC CAATGTTCTT GGTAACTGGG AAAATCTACC TTCCCCTATA 
TCTCCAGACA TTAAGGATTA TAAACTTTAT GATGTAGAAA CCAAATATGG TTTGCTTCAG 
GTTTCTGAAG GATTGTCATT CTTGCATAGC AGTGTGAAAA TGGTGCATGG AAATATCACT 
CCTGAAAATA TAATTTTGAA TAAAAGTGGA GCCTGGAAAA TAATGGGTTT TGATTTTTGT 
GTATCATCAA CCAATCCTTC TGAACAAGAG CCTAAATTTC CTTGTAAAGA ATGGGACCCA 
AATTTACCTT CATTGTGTCT TCCAAATCCT GAATATTTGG CTCCTGAATA CATACTTTCT 
GTGAGCTGTG AAACAGCCAG TGATATGTAT TCTTTAGGAA CTGTTATGTA TGCTGTATTT 
AATAAAGGGA AACCTATATT TGAAGTCAAC AAGCAAGATA TTTACAAGAG TTTCAGTAGG 
CAGTTGGATC AGTTGAGTCG TTTAGGATCT AGTTCACTTA CAAATATACC TGAGGAAGTT 
CGTGAACATG TAAAGCTACT GTTAAATGTA ACTCCGACTG TAAGACCAGA TGCAGATCAA 
ATGACAAAGA TTCCCTTCTT TGATGATGTT GGTGCAGTAA CACTGCAATA TTTTGATACC 
TTATTCCAAA GAGATAATCT TCAGAAATCA CAGTTTTTCA AAGGACTGCC AAAGGTTCTA 
CCAAAACTGC CCAAGCGTGT CATTGTGCAG AGAATTTTGC CTTGTTTGAC TTCAGAATTT 
GTAAACCCTG ACATGGTACC TTTTGTTTTG CCCAATGTTC TACTTATTGC TGAGGAATGC 
ACCAAAGAAG AATATGTCAA ATTAATTCTT CCTGAACTTG GCCCTGTGTT TAAGCAGCAG 
GAGCCAATCC AGGCTAGCAA CATGATTTTG TTAATTTTCC TACAAAAAAT GGATTTGCTA 
CTAACCAAAA CCCCTCCTGA TGAGATAAAG AACAGTGTTC TACCCATGGT TTACAGAGCA 
CTAGAAGCTC CTTCCATTCA GATCCAGGAG CTCTGTCTAA ACATCATTCC AACCTTTGCA 
AATCTTATAG ACTACCCATC CATGAAAAAC GCTTTGATAC CAAGAATTAA AAATGCTTGT 
CTACAAACAT CTTCCCTTGC GGTTCGTGTA AATTCATTAG TGTGCTTAGG AAAGATTTTG 
GAATACTTGG ATAAGTGGTT TGTACTTGAT GATATCCTAC CCTTCTTACA ACAAATTCCA 
TCCAAGGAAC CTGCGGTCCT CATGGGAATT TTAGGTATTT ACAAATGTAC TTTTACTCAT 
AAGAAGTTGG GAATCACCAA AGAGCAGCTG GCCGGAAAAG TGTTGCCTCA TCTTATTCCC 
CTGAGTATTG AAAACAATCT TAATCTTAAT CAGTTCAATT CTTTCATTTC CGTCATAAAA 
GAAATGCTTA ATAGATTGGA GTCTGAACAT AAGACTAAAC TGGAGCAACT TCATATAATG 
CAAGAACAGC AGAAATCTTT GGATATAGGA AATCAAATGA ATGTTTCTGA GGAGATGAAA 
GTTACAAATA TTGGGAATCA GCAAATTGAC AAAGTTTTTA ACAACATTGG AGCAGACCTT 
CTGACTGGCA GTGAGTCCGA AAATAAAGAG GACGGGTTAC AGAATAAACA TAAAAGAGCA 
TCACTTACAC TTGAAGAAAA ACAAAAATTA GCAAAAGAAC AAGAGCAGGC ACAGAAGCTG 
AAAAGCCAGC AGCCTCTTAA ACCCCAAGTG CACACACCTG TTGCTACTGT TAAACAGACT 
AAGGACTTGA CAGACACACT GATGGATAAT ATGTCATCCT TGACCAGCCT TTCTGTTAGT 
ACCCCTAAAT CTTCTGCTTC AAGTACTTTC ACTTCTGTTC CTTCCATGGG CATTGGTATG 
ATGTTTTCTA CACCAACTGA TAATACAAAG AGAAATTTGA CAAATGGCCT AAATGCCAAT 
ATGGGCTTTC AGACTTCAGG ATTCAACATG CCCGTTAATA CAAACCAGAA CTTCTACAGT 
AGTCCAAGCA CAGTTGGAGT GACCAAGATG ACTCTGGGAA CACCTCCCAC TTTGCCAAAC 
TTCAATGCTT TGAGTGTTCC TCCTGCTGGT GCAAAGCAGA CCCAACAAAG ACCCACAGAT 
ATGTCTGCCC TTAATAATCT CTTTGGCCCT CAGAAACCCA AAGTTAGCAT GAACCAGTTA 
TCACAACAGA AACCAAATCA GTGGCTTAAT CAGTTTGTAC CTCCTCAAGG TTCTCCAACT 
ATGGGCAGTT CAGTAATGGG GACACAGATG AACGTGATAG GACAATCTGC TTTTGGTATG 
CAGGGTAATC CTTTCTTTAA CCCACAGAAC TTTGCACAGC CACCAACTAC TATGACCAAT 
AGCAGTTCAG CTAGCAATGA TTTAAAAGAT CTTTTTGGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317183.1
CDS	286..3087
Translation	MESMLNKLKSTVTKVTADVTSAVMGNPVTREFDVGRHIASGGNGLAWKIFNGTKKSTKQEVAVFVFDKKLIDKYQKFEKDQIIDSLKRGVQQLTRLRHPRLLTVQHPLEESRDCLAFCTEPVFASLANVLGNWENLPSPISPDIKDYKLYDVETKYGLLQVSEGLSFLHSSVKMVHGNITPENIILNKSGAWKIMGFDFCVSSTNPSEQEPKFPCKEWDPNLPSLCLPNPEYLAPEYILSVSCETASDMYSLGTVMYAVFNKGKPIFEVNKQDIYKSFSRQLDQLSRLGSSSLTNIPEEVREHVKLLLNVTPTVRPDADQMTKIPFFDDVGAVTLQYFDTLFQRDNLQKSQFFKGLPKVLPKLPKRVIVQRILPCLTSEFVNPDMVPFVLPNVLLIAEECTKEEYVKLILPELGPVFKQQEPIQASNMILLIFLQKMDLLLTKTPPDEIKNSVLPMVYRALEAPSIQIQELCLNIIPTFANLIDYPSMKNALIPRIKNACLQTSSLAVRVNSLVCLGKILEYLDKWFVLDDILPFLQQIPSKEPAVLMGILGIYKCTFTHKKLGITKEQLAGKVLPHLIPLSIENNLNLNQFNSFISVIKEMLNRLESEHKTKLEQLHIMQEQQKSLDIGNQMNVSEEMKVTNIGNQQIDKVFNNIGADLLTGSESENKEDGLQNKHKRASLTLEEKQKLAKEQEQAQKLKSQQPLKPQVHTPVATVKQTKDLTDTLMDNMSSLTSLSVSTPKSSASSTFTSVPSMGIGMMFSTPTDNTKRNLTNGLNANMGFQTSGFNMPVNTNQNFYSSPSTVGVTKMTLGTPPTLPNFNALSVPPAGAKQTQQRPTDMSALNNLFGPQKPKVSMNQLSQQKPNQWLNQFVPPQGSPTMGSSVMGTQMNVIGQSAFGMQGNPFFNPQNFAQPPTTMTNSSSASNDLKDLFG

Target ORF information:

RefSeq Version	NM_001330254.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SCY1 like pseudokinase 2 (SCYL2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330254.1

ORF Insert Sequence:

ATGGAGTCCA TGCTTAATAA ATTGAAGAGT ACTGTTACAA AAGTAACAGC TGATGTCACT 
AGTGCTGTAA TGGGAAATCC TGTCACTAGA GAATTTGATG TTGGTCGACA CATTGCCAGT 
GGTGGCAATG GGCTAGCTTG GAAGATTTTT AATGGCACAA AAAAGTCAAC AAAGCAGGAA 
GTGGCAGTTT TTGTCTTTGA TAAAAAACTG ATTGACAAGT ATCAAAAATT TGAAAAGGAT 
CAAATCATTG ATTCTCTAAA ACGAGGAGTC CAACAGTTAA CTCGGCTTCG ACACCCTCGA 
CTTCTTACTG TCCAGCATCC TTTAGAAGAA TCCAGGGATT GCTTGGCATT TTGTACAGAA 
CCAGTTTTTG CCAGTTTAGC CAATGTTCTT GGTAACTGGG AAAATCTACC TTCCCCTATA 
TCTCCAGACA TTAAGGATTA TAAACTTTAT GATGTAGAAA CCAAATATGG TTTGCTTCAG 
GTTTCTGAAG GATTGTCATT CTTGCATAGC AGTGTGAAAA TGGTGCATGG AAATATCACT 
CCTGAAAATA TAATTTTGAA TAAAAGTGGA GCCTGGAAAA TAATGGGTTT TGATTTTTGT 
GTATCATCAA CCAATCCTTC TGAACAAGAG CCTAAATTTC CTTGTAAAGA ATGGGACCCA 
AATTTACCTT CATTGTGTCT TCCAAATCCT GAATATTTGG CTCCTGAATA CATACTTTCT 
GTGAGCTGTG AAACAGCCAG TGATATGTAT TCTTTAGGAA CTGTTATGTA TGCTGTATTT 
AATAAAGGGA AACCTATATT TGAAGTCAAC AAGCAAGATA TTTACAAGAG TTTCAGTAGG 
CAGTTGGATC AGTTGAGTCG TTTAGGATCT AGTTCACTTA CAAATATACC TGAGGAAGTT 
CGTGAACATG TAAAGCTACT GTTAAATGTA ACTCCGACTG TAAGACCAGA TGCAGATCAA 
ATGACAAAGA TTCCCTTCTT TGATGATGTT GGTGCAGTAA CACTGCAATA TTTTGATACC 
TTATTCCAAA GAGATAATCT TCAGAAATCA CAGTTTTTCA AAGGACTGCC AAAGGTTCTA 
CCAAAACTGC CCAAGCGTGT CATTGTGCAG AGAATTTTGC CTTGTTTGAC TTCAGAATTT 
GTAAACCCTG ACATGGTACC TTTTGTTTTG CCCAATGTTC TACTTATTGC TGAGGAATGC 
ACCAAAGAAG AATATGTCAA ATTAATTCTT CCTGAACTTG GCCCTGTGTT TAAGCAGCAG 
GAGCCAATCC AGGCTAGCAA CATGATTTTG TTAATTTTCC TACAAAAAAT GGATTTGCTA 
CTAACCAAAA CCCCTCCTGA TGAGATAAAG AACAGTGTTC TACCCATGGT TTACAGAGCA 
CTAGAAGCTC CTTCCATTCA GATCCAGGAG CTCTGTCTAA ACATCATTCC AACCTTTGCA 
AATCTTATAG ACTACCCATC CATGAAAAAC GCTTTGATAC CAAGAATTAA AAATGCTTGT 
CTACAAACAT CTTCCCTTGC GGTTCGTGTA AATTCATTAG TGTGCTTAGG AAAGATTTTG 
GAATACTTGG ATAAGTGGTT TGTACTTGAT GATATCCTAC CCTTCTTACA ACAAATTCCA 
TCCAAGGAAC CTGCGGTCCT CATGGGAATT TTAGGTATTT ACAAATGTAC TTTTACTCAT 
AAGAAGTTGG GAATCACCAA AGAGCAGCTG GCCGGAAAAG TGTTGCCTCA TCTTATTCCC 
CTGAGTATTG AAAACAATCT TAATCTTAAT CAGTTCAATT CTTTCATTTC CGTCATAAAA 
GAAATGCTTA ATAGATTGGA GTCTGAACAT AAGACTAAAC TGGAGCAACT TCATATAATG 
CAAGAACAGC AGAAATCTTT GGATATAGGA AATCAAATGA ATGTTTCTGA GGAGATGAAA 
GTTACAAATA TTGGGAATCA GCAAATTGAC AAAGTTTTTA ACAACATTGG AGCAGACCTT 
CTGACTGGCA GTGAGTCCGA AAATAAAGAG GACGGGTTAC AGAATAAACA TAAAAGAGCA 
TCACTTACAC TTGAAGAAAA ACAAAAATTA GCAAAAGAAC AAGAGCAGGC ACAGAAGCTG 
AAAAGCCAGC AGCCTCTTAA ACCCCAAGTG CACACACCTG TTGCTACTGT TAAACAGACT 
AAGGACTTGA CAGACACACT GATGGATAAT ATGTCATCCT TGACCAGCCT TTCTGTTAGT 
ACCCCTAAAT CTTCTGCTTC AAGTACTTTC ACTTCTGTTC CTTCCATGGG CATTGGTATG 
ATGTTTTCTA CACCAACTGA TAATACAAAG AGAAATTTGA CAAATGGCCT AAATGCCAAT 
ATGGGCTTTC AGACTTCAGG ATTCAACATG CCCGTTAATA CAAACCAGAA CTTCTACAGT 
AGTCCAAGCA CAGTTGGAGT GACCAAGATG ACTCTGGGAA CACCTCCCAC TTTGCCAAAC 
TTCAATGCTT TGAGTGTTCC TCCTGCTGGT GCAAAGCAGA CCCAACAAAG ACCCACAGAT 
ATGTCTGCCC TTAATAATCT CTTTGGCCCT CAGAAACCCA AAGTTAGCAT GAACCAGTTA 
TCACAACAGA AACCAAATCA GTGGCTTAAT CAGTTTGTAC CTCCTCAAGG TTCTCCAACT 
ATGGGCAGTT CAGTAATGGG GACACAGATG AACGTGATAG GACAATCTGC TTTTGGTATG 
CAGGGTAATC CTTTCTTTAA CCCACAGAAC TTTGCACAGC CACCAACTAC TATGACCAAT 
AGCAGTTCAG CTAGCAATGA TTTAAAAGAT CTTTTTGGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCYL2 ( NM_001330253.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317182.1 Homo sapiens SCY1 like pseudokinase 2 (SCYL2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu101275
Clone ID Related Accession (Same CDS sequence)	NM_001330254.1 , NM_001330253.1
Accession Version	NM_001330253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	SCY1-like protein 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB127407.1, BC063798.1, AB037781.1 and CN337316.1. On Aug 20, 2016 this sequence version replaced XM_017019619.1. Transcript Variant: This variant (3) contains an alternate in-frame exon in its central coding region, compared to variant 1. The encoded isoform (2) has the same N- and C-termini, but is longer than isoform 1. Both variants 3 and 4 encode the same isoform (2). ##Evidence-Data-START## Transcript exon combination :: SRR1660805.244810.1, SRR1803612.195259.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTCCA TGCTTAATAA ATTGAAGAGT ACTGTTACAA AAGTAACAGC TGATGTCACT 
AGTGCTGTAA TGGGAAATCC TGTCACTAGA GAATTTGATG TTGGTCGACA CATTGCCAGT 
GGTGGCAATG GGCTAGCTTG GAAGATTTTT AATGGCACAA AAAAGTCAAC AAAGCAGGAA 
GTGGCAGTTT TTGTCTTTGA TAAAAAACTG ATTGACAAGT ATCAAAAATT TGAAAAGGAT 
CAAATCATTG ATTCTCTAAA ACGAGGAGTC CAACAGTTAA CTCGGCTTCG ACACCCTCGA 
CTTCTTACTG TCCAGCATCC TTTAGAAGAA TCCAGGGATT GCTTGGCATT TTGTACAGAA 
CCAGTTTTTG CCAGTTTAGC CAATGTTCTT GGTAACTGGG AAAATCTACC TTCCCCTATA 
TCTCCAGACA TTAAGGATTA TAAACTTTAT GATGTAGAAA CCAAATATGG TTTGCTTCAG 
GTTTCTGAAG GATTGTCATT CTTGCATAGC AGTGTGAAAA TGGTGCATGG AAATATCACT 
CCTGAAAATA TAATTTTGAA TAAAAGTGGA GCCTGGAAAA TAATGGGTTT TGATTTTTGT 
GTATCATCAA CCAATCCTTC TGAACAAGAG CCTAAATTTC CTTGTAAAGA ATGGGACCCA 
AATTTACCTT CATTGTGTCT TCCAAATCCT GAATATTTGG CTCCTGAATA CATACTTTCT 
GTGAGCTGTG AAACAGCCAG TGATATGTAT TCTTTAGGAA CTGTTATGTA TGCTGTATTT 
AATAAAGGGA AACCTATATT TGAAGTCAAC AAGCAAGATA TTTACAAGAG TTTCAGTAGG 
CAGTTGGATC AGTTGAGTCG TTTAGGATCT AGTTCACTTA CAAATATACC TGAGGAAGTT 
CGTGAACATG TAAAGCTACT GTTAAATGTA ACTCCGACTG TAAGACCAGA TGCAGATCAA 
ATGACAAAGA TTCCCTTCTT TGATGATGTT GGTGCAGTAA CACTGCAATA TTTTGATACC 
TTATTCCAAA GAGATAATCT TCAGAAATCA CAGTTTTTCA AAGGACTGCC AAAGGTTCTA 
CCAAAACTGC CCAAGCGTGT CATTGTGCAG AGAATTTTGC CTTGTTTGAC TTCAGAATTT 
GTAAACCCTG ACATGGTACC TTTTGTTTTG CCCAATGTTC TACTTATTGC TGAGGAATGC 
ACCAAAGAAG AATATGTCAA ATTAATTCTT CCTGAACTTG GCCCTGTGTT TAAGCAGCAG 
GAGCCAATCC AGGCTAGCAA CATGATTTTG TTAATTTTCC TACAAAAAAT GGATTTGCTA 
CTAACCAAAA CCCCTCCTGA TGAGATAAAG AACAGTGTTC TACCCATGGT TTACAGAGCA 
CTAGAAGCTC CTTCCATTCA GATCCAGGAG CTCTGTCTAA ACATCATTCC AACCTTTGCA 
AATCTTATAG ACTACCCATC CATGAAAAAC GCTTTGATAC CAAGAATTAA AAATGCTTGT 
CTACAAACAT CTTCCCTTGC GGTTCGTGTA AATTCATTAG TGTGCTTAGG AAAGATTTTG 
GAATACTTGG ATAAGTGGTT TGTACTTGAT GATATCCTAC CCTTCTTACA ACAAATTCCA 
TCCAAGGAAC CTGCGGTCCT CATGGGAATT TTAGGTATTT ACAAATGTAC TTTTACTCAT 
AAGAAGTTGG GAATCACCAA AGAGCAGCTG GCCGGAAAAG TGTTGCCTCA TCTTATTCCC 
CTGAGTATTG AAAACAATCT TAATCTTAAT CAGTTCAATT CTTTCATTTC CGTCATAAAA 
GAAATGCTTA ATAGATTGGA GTCTGAACAT AAGACTAAAC TGGAGCAACT TCATATAATG 
CAAGAACAGC AGAAATCTTT GGATATAGGA AATCAAATGA ATGTTTCTGA GGAGATGAAA 
GTTACAAATA TTGGGAATCA GCAAATTGAC AAAGTTTTTA ACAACATTGG AGCAGACCTT 
CTGACTGGCA GTGAGTCCGA AAATAAAGAG GACGGGTTAC AGAATAAACA TAAAAGAGCA 
TCACTTACAC TTGAAGAAAA ACAAAAATTA GCAAAAGAAC AAGAGCAGGC ACAGAAGCTG 
AAAAGCCAGC AGCCTCTTAA ACCCCAAGTG CACACACCTG TTGCTACTGT TAAACAGACT 
AAGGACTTGA CAGACACACT GATGGATAAT ATGTCATCCT TGACCAGCCT TTCTGTTAGT 
ACCCCTAAAT CTTCTGCTTC AAGTACTTTC ACTTCTGTTC CTTCCATGGG CATTGGTATG 
ATGTTTTCTA CACCAACTGA TAATACAAAG AGAAATTTGA CAAATGGCCT AAATGCCAAT 
ATGGGCTTTC AGACTTCAGG ATTCAACATG CCCGTTAATA CAAACCAGAA CTTCTACAGT 
AGTCCAAGCA CAGTTGGAGT GACCAAGATG ACTCTGGGAA CACCTCCCAC TTTGCCAAAC 
TTCAATGCTT TGAGTGTTCC TCCTGCTGGT GCAAAGCAGA CCCAACAAAG ACCCACAGAT 
ATGTCTGCCC TTAATAATCT CTTTGGCCCT CAGAAACCCA AAGTTAGCAT GAACCAGTTA 
TCACAACAGA AACCAAATCA GTGGCTTAAT CAGTTTGTAC CTCCTCAAGG TTCTCCAACT 
ATGGGCAGTT CAGTAATGGG GACACAGATG AACGTGATAG GACAATCTGC TTTTGGTATG 
CAGGGTAATC CTTTCTTTAA CCCACAGAAC TTTGCACAGC CACCAACTAC TATGACCAAT 
AGCAGTTCAG CTAGCAATGA TTTAAAAGAT CTTTTTGGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317182.1
CDS	438..3239
Translation	MESMLNKLKSTVTKVTADVTSAVMGNPVTREFDVGRHIASGGNGLAWKIFNGTKKSTKQEVAVFVFDKKLIDKYQKFEKDQIIDSLKRGVQQLTRLRHPRLLTVQHPLEESRDCLAFCTEPVFASLANVLGNWENLPSPISPDIKDYKLYDVETKYGLLQVSEGLSFLHSSVKMVHGNITPENIILNKSGAWKIMGFDFCVSSTNPSEQEPKFPCKEWDPNLPSLCLPNPEYLAPEYILSVSCETASDMYSLGTVMYAVFNKGKPIFEVNKQDIYKSFSRQLDQLSRLGSSSLTNIPEEVREHVKLLLNVTPTVRPDADQMTKIPFFDDVGAVTLQYFDTLFQRDNLQKSQFFKGLPKVLPKLPKRVIVQRILPCLTSEFVNPDMVPFVLPNVLLIAEECTKEEYVKLILPELGPVFKQQEPIQASNMILLIFLQKMDLLLTKTPPDEIKNSVLPMVYRALEAPSIQIQELCLNIIPTFANLIDYPSMKNALIPRIKNACLQTSSLAVRVNSLVCLGKILEYLDKWFVLDDILPFLQQIPSKEPAVLMGILGIYKCTFTHKKLGITKEQLAGKVLPHLIPLSIENNLNLNQFNSFISVIKEMLNRLESEHKTKLEQLHIMQEQQKSLDIGNQMNVSEEMKVTNIGNQQIDKVFNNIGADLLTGSESENKEDGLQNKHKRASLTLEEKQKLAKEQEQAQKLKSQQPLKPQVHTPVATVKQTKDLTDTLMDNMSSLTSLSVSTPKSSASSTFTSVPSMGIGMMFSTPTDNTKRNLTNGLNANMGFQTSGFNMPVNTNQNFYSSPSTVGVTKMTLGTPPTLPNFNALSVPPAGAKQTQQRPTDMSALNNLFGPQKPKVSMNQLSQQKPNQWLNQFVPPQGSPTMGSSVMGTQMNVIGQSAFGMQGNPFFNPQNFAQPPTTMTNSSSASNDLKDLFG

Target ORF information:

RefSeq Version	NM_001330253.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SCY1 like pseudokinase 2 (SCYL2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330253.1

ORF Insert Sequence:

ATGGAGTCCA TGCTTAATAA ATTGAAGAGT ACTGTTACAA AAGTAACAGC TGATGTCACT 
AGTGCTGTAA TGGGAAATCC TGTCACTAGA GAATTTGATG TTGGTCGACA CATTGCCAGT 
GGTGGCAATG GGCTAGCTTG GAAGATTTTT AATGGCACAA AAAAGTCAAC AAAGCAGGAA 
GTGGCAGTTT TTGTCTTTGA TAAAAAACTG ATTGACAAGT ATCAAAAATT TGAAAAGGAT 
CAAATCATTG ATTCTCTAAA ACGAGGAGTC CAACAGTTAA CTCGGCTTCG ACACCCTCGA 
CTTCTTACTG TCCAGCATCC TTTAGAAGAA TCCAGGGATT GCTTGGCATT TTGTACAGAA 
CCAGTTTTTG CCAGTTTAGC CAATGTTCTT GGTAACTGGG AAAATCTACC TTCCCCTATA 
TCTCCAGACA TTAAGGATTA TAAACTTTAT GATGTAGAAA CCAAATATGG TTTGCTTCAG 
GTTTCTGAAG GATTGTCATT CTTGCATAGC AGTGTGAAAA TGGTGCATGG AAATATCACT 
CCTGAAAATA TAATTTTGAA TAAAAGTGGA GCCTGGAAAA TAATGGGTTT TGATTTTTGT 
GTATCATCAA CCAATCCTTC TGAACAAGAG CCTAAATTTC CTTGTAAAGA ATGGGACCCA 
AATTTACCTT CATTGTGTCT TCCAAATCCT GAATATTTGG CTCCTGAATA CATACTTTCT 
GTGAGCTGTG AAACAGCCAG TGATATGTAT TCTTTAGGAA CTGTTATGTA TGCTGTATTT 
AATAAAGGGA AACCTATATT TGAAGTCAAC AAGCAAGATA TTTACAAGAG TTTCAGTAGG 
CAGTTGGATC AGTTGAGTCG TTTAGGATCT AGTTCACTTA CAAATATACC TGAGGAAGTT 
CGTGAACATG TAAAGCTACT GTTAAATGTA ACTCCGACTG TAAGACCAGA TGCAGATCAA 
ATGACAAAGA TTCCCTTCTT TGATGATGTT GGTGCAGTAA CACTGCAATA TTTTGATACC 
TTATTCCAAA GAGATAATCT TCAGAAATCA CAGTTTTTCA AAGGACTGCC AAAGGTTCTA 
CCAAAACTGC CCAAGCGTGT CATTGTGCAG AGAATTTTGC CTTGTTTGAC TTCAGAATTT 
GTAAACCCTG ACATGGTACC TTTTGTTTTG CCCAATGTTC TACTTATTGC TGAGGAATGC 
ACCAAAGAAG AATATGTCAA ATTAATTCTT CCTGAACTTG GCCCTGTGTT TAAGCAGCAG 
GAGCCAATCC AGGCTAGCAA CATGATTTTG TTAATTTTCC TACAAAAAAT GGATTTGCTA 
CTAACCAAAA CCCCTCCTGA TGAGATAAAG AACAGTGTTC TACCCATGGT TTACAGAGCA 
CTAGAAGCTC CTTCCATTCA GATCCAGGAG CTCTGTCTAA ACATCATTCC AACCTTTGCA 
AATCTTATAG ACTACCCATC CATGAAAAAC GCTTTGATAC CAAGAATTAA AAATGCTTGT 
CTACAAACAT CTTCCCTTGC GGTTCGTGTA AATTCATTAG TGTGCTTAGG AAAGATTTTG 
GAATACTTGG ATAAGTGGTT TGTACTTGAT GATATCCTAC CCTTCTTACA ACAAATTCCA 
TCCAAGGAAC CTGCGGTCCT CATGGGAATT TTAGGTATTT ACAAATGTAC TTTTACTCAT 
AAGAAGTTGG GAATCACCAA AGAGCAGCTG GCCGGAAAAG TGTTGCCTCA TCTTATTCCC 
CTGAGTATTG AAAACAATCT TAATCTTAAT CAGTTCAATT CTTTCATTTC CGTCATAAAA 
GAAATGCTTA ATAGATTGGA GTCTGAACAT AAGACTAAAC TGGAGCAACT TCATATAATG 
CAAGAACAGC AGAAATCTTT GGATATAGGA AATCAAATGA ATGTTTCTGA GGAGATGAAA 
GTTACAAATA TTGGGAATCA GCAAATTGAC AAAGTTTTTA ACAACATTGG AGCAGACCTT 
CTGACTGGCA GTGAGTCCGA AAATAAAGAG GACGGGTTAC AGAATAAACA TAAAAGAGCA 
TCACTTACAC TTGAAGAAAA ACAAAAATTA GCAAAAGAAC AAGAGCAGGC ACAGAAGCTG 
AAAAGCCAGC AGCCTCTTAA ACCCCAAGTG CACACACCTG TTGCTACTGT TAAACAGACT 
AAGGACTTGA CAGACACACT GATGGATAAT ATGTCATCCT TGACCAGCCT TTCTGTTAGT 
ACCCCTAAAT CTTCTGCTTC AAGTACTTTC ACTTCTGTTC CTTCCATGGG CATTGGTATG 
ATGTTTTCTA CACCAACTGA TAATACAAAG AGAAATTTGA CAAATGGCCT AAATGCCAAT 
ATGGGCTTTC AGACTTCAGG ATTCAACATG CCCGTTAATA CAAACCAGAA CTTCTACAGT 
AGTCCAAGCA CAGTTGGAGT GACCAAGATG ACTCTGGGAA CACCTCCCAC TTTGCCAAAC 
TTCAATGCTT TGAGTGTTCC TCCTGCTGGT GCAAAGCAGA CCCAACAAAG ACCCACAGAT 
ATGTCTGCCC TTAATAATCT CTTTGGCCCT CAGAAACCCA AAGTTAGCAT GAACCAGTTA 
TCACAACAGA AACCAAATCA GTGGCTTAAT CAGTTTGTAC CTCCTCAAGG TTCTCCAACT 
ATGGGCAGTT CAGTAATGGG GACACAGATG AACGTGATAG GACAATCTGC TTTTGGTATG 
CAGGGTAATC CTTTCTTTAA CCCACAGAAC TTTGCACAGC CACCAACTAC TATGACCAAT 
AGCAGTTCAG CTAGCAATGA TTTAAAAGAT CTTTTTGGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCYL2 ( NM_001330256.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317185.1 Homo sapiens SCY1 like pseudokinase 2 (SCYL2), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu101276
Clone ID Related Accession (Same CDS sequence)	NM_001330256.1
Accession Version	NM_001330256.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	SCY1-like protein 2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA234206.1, DB261340.1, AC026110.42, BC011525.1, AB037781.1 and CN337316.1. On Aug 20, 2016 this sequence version replaced XM_017019620.1. Transcript Variant: This variant (5) differs in the 5' UTR and contains two alternate exons compared to variant 1. This variant represents translation initiation at a downstream start codon compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream start codon to encode an isoform (3) that has a shorter N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.165568.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCATG GAAATATCAC TCCTGAAAAT ATAATTTTGA ATAAAAGTGG AGCCTGGAAA 
ATAATGGGTT TTGATTTTTG TGTATCATCA ACCAATCCTT CTGAACAAGA GCCTAAATTT 
CCTTGTAAAG AATGGGACCC AAATTTACCT TCATTGTGTC TTCCAAATCC TGAATATTTG 
GCTCCTGAAT ACATACTTTC TGTGAGCTGT GAAACAGCCA GTGATATGTA TTCTTTAGGA 
ACTGTTATGT ATGCTGTATT TAATAAAGGG AAACCTATAT TTGAAGTCAA CAAGCAAGAT 
ATTTACAAGA GTTTCAGTAG GCAGTTGGAT CAGTTGAGTC GTTTAGGATC TAGTTCACTT 
ACAAATATAC CTGAGGAAGT TCGTGAACAT GTAAAGCTAC TGTTAAATGT AACTCCGACT 
GTAAGACCAG ATGCAGATCA AATGACAAAG ATTCCCTTCT TTGATGATGT TGGTGCAGTA 
ACACTGCAAT ATTTTGATAC CTTATTCCAA AGAGATAATC TTCAGAAATC ACAGTTTTTC 
AAAGGACTGC CAAAGGTTCT ACCAAAACTG CCCAAGCGTG TCATTGTGCA GAGAATTTTG 
CCTTGTTTGA CTTCAGAATT TGTAAACCCT GACATGGTAC CTTTTGTTTT GCCCAATGTT 
CTACTTATTG CTGAGGAATG CACCAAAGAA GAATATGTCA AATTAATTCT TCCTGAACTT 
GGCCCTGTGT TTAAGCAGCA GGAGCCAATC CAGGCTAGCA ACATGATTTT GTTAATTTTC 
CTACAAAAAA TGGATTTGCT ACTAACCAAA ACCCCTCCTG ATGAGATAAA GAACAGTGTT 
CTACCCATGG TTTACAGAGC ACTAGAAGCT CCTTCCATTC AGATCCAGGA GCTCTGTCTA 
AACATCATTC CAACCTTTGC AAATCTTATA GACTACCCAT CCATGAAAAA CGCTTTGATA 
CCAAGAATTA AAAATGCTTG TCTACAAACA TCTTCCCTTG CGGTTCGTGT AAATTCATTA 
GTGTGCTTAG GAAAGATTTT GGAATACTTG GATAAGTGGT TTGTACTTGA TGATATCCTA 
CCCTTCTTAC AACAAATTCC ATCCAAGGAA CCTGCGGTCC TCATGGGAAT TTTAGGTATT 
TACAAATGTA CTTTTACTCA TAAGAAGTTG GGAATCACCA AAGAGCAGCT GGCCGGAAAA 
GTGTTGCCTC ATCTTATTCC CCTGAGTATT GAAAACAATC TTAATCTTAA TCAGTTCAAT 
TCTTTCATTT CCGTCATAAA AGAAATGCTT AATAGATTGG AGTCTGAACA TAAGACTAAA 
CTGGAGCAAC TTCATATAAT GCAAGAACAG CAGAAATCTT TGGATATAGG AAATCAAATG 
AATGTTTCTG AGGAGATGAA AGTTACAAAT ATTGGGAATC AGCAAATTGA CAAAGTTTTT 
AACAACATTG GAGCAGACCT TCTGACTGGC AGTGAGTCCG AAAATAAAGA GGACGGGTTA 
CAGAATAAAC ATAAAAGAGC ATCACTTACA CTTGAAGAAA AACAAAAATT AGCAAAAGAA 
CAAGAGCAGG CACAGAAGCT GAAAAGCCAG CAGCCTCTTA AACCCCAAGT GCACACACCT 
GTTGCTACTG TTAAACAGAC TAAGGACTTG ACAGACACAC TGATGGATAA TATGTCATCC 
TTGACCAGCC TTTCTGTTAG TACCCCTAAA TCTTCTGCTT CAAGTACTTT CACTTCTGTT 
CCTTCCATGG GCATTGGTAT GATGTTTTCT ACACCAACTG ATAATACAAA GAGAAATTTG 
ACAAATGGCC TAAATGCCAA TATGGGCTTT CAGACTTCAG GATTCAACAT GCCCGTTAAT 
ACAAACCAGA ACTTCTACAG TAGTCCAAGC ACAGTTGGAG TGACCAAGAT GACTCTGGGA 
ACACCTCCCA CTTTGCCAAA CTTCAATGCT TTGAGTGTTC CTCCTGCTGG TGCAAAGCAG 
ACCCAACAAA GACCCACAGA TATGTCTGCC CTTAATAATC TCTTTGGCCC TCAGAAACCC 
AAAGTTAGCA TGAACCAGTT ATCACAACAG AAACCAAATC AGTGGCTTAA TCAGTTTGTA 
CCTCCTCAAG GTTCTCCAAC TATGGGCAGT TCAGTAATGG GGACACAGAT GAACGTGATA 
GGACAATCTG CTTTTGGTAT GCAGGGTAAT CCTTTCTTTA ACCCACAGAA CTTTGCACAG 
CCACCAACTA CTATGACCAA TAGCAGTTCA GCTAGCAATG ATTTAAAAGA TCTTTTTGGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317185.1
CDS	941..3223
Translation	MVHGNITPENIILNKSGAWKIMGFDFCVSSTNPSEQEPKFPCKEWDPNLPSLCLPNPEYLAPEYILSVSCETASDMYSLGTVMYAVFNKGKPIFEVNKQDIYKSFSRQLDQLSRLGSSSLTNIPEEVREHVKLLLNVTPTVRPDADQMTKIPFFDDVGAVTLQYFDTLFQRDNLQKSQFFKGLPKVLPKLPKRVIVQRILPCLTSEFVNPDMVPFVLPNVLLIAEECTKEEYVKLILPELGPVFKQQEPIQASNMILLIFLQKMDLLLTKTPPDEIKNSVLPMVYRALEAPSIQIQELCLNIIPTFANLIDYPSMKNALIPRIKNACLQTSSLAVRVNSLVCLGKILEYLDKWFVLDDILPFLQQIPSKEPAVLMGILGIYKCTFTHKKLGITKEQLAGKVLPHLIPLSIENNLNLNQFNSFISVIKEMLNRLESEHKTKLEQLHIMQEQQKSLDIGNQMNVSEEMKVTNIGNQQIDKVFNNIGADLLTGSESENKEDGLQNKHKRASLTLEEKQKLAKEQEQAQKLKSQQPLKPQVHTPVATVKQTKDLTDTLMDNMSSLTSLSVSTPKSSASSTFTSVPSMGIGMMFSTPTDNTKRNLTNGLNANMGFQTSGFNMPVNTNQNFYSSPSTVGVTKMTLGTPPTLPNFNALSVPPAGAKQTQQRPTDMSALNNLFGPQKPKVSMNQLSQQKPNQWLNQFVPPQGSPTMGSSVMGTQMNVIGQSAFGMQGNPFFNPQNFAQPPTTMTNSSSASNDLKDLFG

Target ORF information:

RefSeq Version	NM_001330256.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SCY1 like pseudokinase 2 (SCYL2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330256.1

ORF Insert Sequence:

ATGGTGCATG GAAATATCAC TCCTGAAAAT ATAATTTTGA ATAAAAGTGG AGCCTGGAAA 
ATAATGGGTT TTGATTTTTG TGTATCATCA ACCAATCCTT CTGAACAAGA GCCTAAATTT 
CCTTGTAAAG AATGGGACCC AAATTTACCT TCATTGTGTC TTCCAAATCC TGAATATTTG 
GCTCCTGAAT ACATACTTTC TGTGAGCTGT GAAACAGCCA GTGATATGTA TTCTTTAGGA 
ACTGTTATGT ATGCTGTATT TAATAAAGGG AAACCTATAT TTGAAGTCAA CAAGCAAGAT 
ATTTACAAGA GTTTCAGTAG GCAGTTGGAT CAGTTGAGTC GTTTAGGATC TAGTTCACTT 
ACAAATATAC CTGAGGAAGT TCGTGAACAT GTAAAGCTAC TGTTAAATGT AACTCCGACT 
GTAAGACCAG ATGCAGATCA AATGACAAAG ATTCCCTTCT TTGATGATGT TGGTGCAGTA 
ACACTGCAAT ATTTTGATAC CTTATTCCAA AGAGATAATC TTCAGAAATC ACAGTTTTTC 
AAAGGACTGC CAAAGGTTCT ACCAAAACTG CCCAAGCGTG TCATTGTGCA GAGAATTTTG 
CCTTGTTTGA CTTCAGAATT TGTAAACCCT GACATGGTAC CTTTTGTTTT GCCCAATGTT 
CTACTTATTG CTGAGGAATG CACCAAAGAA GAATATGTCA AATTAATTCT TCCTGAACTT 
GGCCCTGTGT TTAAGCAGCA GGAGCCAATC CAGGCTAGCA ACATGATTTT GTTAATTTTC 
CTACAAAAAA TGGATTTGCT ACTAACCAAA ACCCCTCCTG ATGAGATAAA GAACAGTGTT 
CTACCCATGG TTTACAGAGC ACTAGAAGCT CCTTCCATTC AGATCCAGGA GCTCTGTCTA 
AACATCATTC CAACCTTTGC AAATCTTATA GACTACCCAT CCATGAAAAA CGCTTTGATA 
CCAAGAATTA AAAATGCTTG TCTACAAACA TCTTCCCTTG CGGTTCGTGT AAATTCATTA 
GTGTGCTTAG GAAAGATTTT GGAATACTTG GATAAGTGGT TTGTACTTGA TGATATCCTA 
CCCTTCTTAC AACAAATTCC ATCCAAGGAA CCTGCGGTCC TCATGGGAAT TTTAGGTATT 
TACAAATGTA CTTTTACTCA TAAGAAGTTG GGAATCACCA AAGAGCAGCT GGCCGGAAAA 
GTGTTGCCTC ATCTTATTCC CCTGAGTATT GAAAACAATC TTAATCTTAA TCAGTTCAAT 
TCTTTCATTT CCGTCATAAA AGAAATGCTT AATAGATTGG AGTCTGAACA TAAGACTAAA 
CTGGAGCAAC TTCATATAAT GCAAGAACAG CAGAAATCTT TGGATATAGG AAATCAAATG 
AATGTTTCTG AGGAGATGAA AGTTACAAAT ATTGGGAATC AGCAAATTGA CAAAGTTTTT 
AACAACATTG GAGCAGACCT TCTGACTGGC AGTGAGTCCG AAAATAAAGA GGACGGGTTA 
CAGAATAAAC ATAAAAGAGC ATCACTTACA CTTGAAGAAA AACAAAAATT AGCAAAAGAA 
CAAGAGCAGG CACAGAAGCT GAAAAGCCAG CAGCCTCTTA AACCCCAAGT GCACACACCT 
GTTGCTACTG TTAAACAGAC TAAGGACTTG ACAGACACAC TGATGGATAA TATGTCATCC 
TTGACCAGCC TTTCTGTTAG TACCCCTAAA TCTTCTGCTT CAAGTACTTT CACTTCTGTT 
CCTTCCATGG GCATTGGTAT GATGTTTTCT ACACCAACTG ATAATACAAA GAGAAATTTG 
ACAAATGGCC TAAATGCCAA TATGGGCTTT CAGACTTCAG GATTCAACAT GCCCGTTAAT 
ACAAACCAGA ACTTCTACAG TAGTCCAAGC ACAGTTGGAG TGACCAAGAT GACTCTGGGA 
ACACCTCCCA CTTTGCCAAA CTTCAATGCT TTGAGTGTTC CTCCTGCTGG TGCAAAGCAG 
ACCCAACAAA GACCCACAGA TATGTCTGCC CTTAATAATC TCTTTGGCCC TCAGAAACCC 
AAAGTTAGCA TGAACCAGTT ATCACAACAG AAACCAAATC AGTGGCTTAA TCAGTTTGTA 
CCTCCTCAAG GTTCTCCAAC TATGGGCAGT TCAGTAATGG GGACACAGAT GAACGTGATA 
GGACAATCTG CTTTTGGTAT GCAGGGTAAT CCTTTCTTTA ACCCACAGAA CTTTGCACAG 
CCACCAACTA CTATGACCAA TAGCAGTTCA GCTAGCAATG ATTTAAAAGA TCTTTTTGGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Interferon-induced SCYL2 limits release of HIV-1 by triggering PP2A-mediated dephosphorylation of the viral protein Vpu.
Science signaling5(245)ra73(2012 Oct)
Miyakawa K,Sawasaki T,Matsunaga S,Tokarev A,Quinn G,Kimura H,Nomaguchi M,Adachi A,Yamamoto N,Guatelli J,Ryo A

A coated vesicle-associated kinase of 104 kDa (CVAK104) induces lysosomal degradation of frizzled 5 (Fzd5).
The Journal of biological chemistry284(39)26716-24(2009 Sep)
Terabayashi T,Funato Y,Fukuda M,Miki H

CVAK104 is a novel regulator of clathrin-mediated SNARE sorting.
Traffic (Copenhagen, Denmark)8(7)893-903(2007 Jul)
Borner GH,Rana AA,Forster R,Harbour M,Smith JC,Robinson MS

Clathrin-dependent association of CVAK104 with endosomes and the trans-Golgi network.
Molecular biology of the cell17(10)4513-25(2006 Oct)
D?wel M,Ungewickell EJ

Role of the AP2 beta-appendage hub in recruiting partners for clathrin-coated vesicle assembly.
PLoS biology4(9)e262(2006 Sep)
Schmid EM,Ford MG,Burtey A,Praefcke GJ,Peak-Chew SY,Mills IG,Benmerah A,McMahon HT

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SCYL2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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