PRKACG cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRKACG
Entrez Gene ID	5568
Full Name	protein kinase cAMP-activated catalytic subunit gamma
Synonyms	BDPLT19,KAPG,PKACg
General protein information	Preferred Names protein kinase cAMP-activated catalytic subunit gamma Names cAMP-dependent protein kinase catalytic subunit gamma PKA C-gamma protein kinase, cAMP-dependent, catalytic, gamma protein kinase, cAMP-dependent, gamma catalytic subunit serine(threonine) protein kinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q21.11
Summary	Cyclic AMP-dependent protein kinase (PKA) consists of two catalytic subunits and a regulatory subunit dimer. This gene encodes the gamma form of its catalytic subunit. The gene is intronless and is thought to be a retrotransposon derived from the gene for the alpha form of the PKA catalytic subunit. [provided by RefSeq, Jul 2008].
Disorder MIM:	176893
Disorder Html:	?Bleeding disorder, platelet-type, 19, 616176 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_002732.3	NP_002723.2	cAMP-dependent protein kinase catalytic subunit gamma

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	prkacaa	NP_001076309.1
Kluyveromyces lactis	KLLA0B07205g	XP_451851.1
Pan troglodytes (chimpanzee)	PRKACG	XP_528314.3
Eremothecium gossypii	AGOS_AEL115C	NP_984746.2
Homo sapiens (human)	PRKACG	NP_002723.2
Saccharomyces cerevisiae (baker's yeast)	TPK3	NP_012755.1

Gene Ontology
Bibliography

Related articles in PubMed

A new form of macrothrombocytopenia induced by a germ-line mutation in the PRKACG gene.
Manchev VT, Hilpert M, Berrou E, Elaib Z, Aouba A, Boukour S, Souquere S, Pierron G, Rameau P, Andrews R, Lanza F, Bobe R, Vainchenker W, Rosa JP, Bryckaert M, Debili N, Favier R, Raslova H
Blood124(16)2554-63(2014 Oct)

Overexpressing PKIB in prostate cancer promotes its aggressiveness by linking between PKA and Akt pathways.
Chung S, Furihata M, Tamura K, Uemura M, Daigo Y, Nasu Y, Miki T, Shuin T, Fujioka T, Nakamura Y, Nakagawa H
Oncogene28(32)2849-59(2009 Aug)

PKA-mediated stabilization of FoxH1 negatively regulates ERalpha activity.
Yum J, Jeong HM, Kim S, Seo JW, Han Y, Lee KY, Yeo CY
Molecules and cells28(1)67-71(2009 Jul)

Ser276 phosphorylation of NF-kB p65 by MSK1 controls SCF expression in inflammation.
Reber L, Vermeulen L, Haegeman G, Frossard N
PloS one4(2)e4393(2009)

Human immunodeficiency virus proteins induce the inhibitory cAMP/protein kinase A pathway in normal lymphocytes.
Hofmann B, Nishanian P, Nguyen T, Insixiengmay P, Fahey JL
Proceedings of the National Academy of Sciences of the United States of America90(14)6676-80(1993 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The missense p.74Ile>Met PRKACG mutation is associated with a marked defect in proplatelet formation and a low level in filamin A in megakaryocytes.
Title: A new form of macrothrombocytopenia induced by a germ-line mutation in the PRKACG gene.

Findings show that PKIB and PKA-C kinase can have critical functions of aggressive phenotype of PCs through Akt phosphorylation and that they should be a promising molecular target for PC treatment.
Title: Overexpressing PKIB in prostate cancer promotes its aggressiveness by linking between PKA and Akt pathways.

Results suggest that PKA can negatively regulate ERalpha, at least in part, through FoxH1.
Title: PKA-mediated stabilization of FoxH1 negatively regulates ERalpha activity.

In human primary pigmented nodular adrenocortical disease tissues, dexamethasone paradoxically stimulates cortisol release through a glucocorticoid receptor-mediated effect on PKA catalytic subunits.
Title: The paradoxical increase in cortisol secretion induced by dexamethasone in primary pigmented nodular adrenocortical disease involves a glucocorticoid receptor-mediated effect of dexamethasone on protein kinase A catalytic subunits.

Results suggest that Cgamma and Calpha differ in structure and function in vitro, supporting the hypothesis that functionally different C-subunit isozymes could affect cAMP signal transduction downstream of protein kinase A activation.
Title: Characterization of the cAMP-dependent protein kinase catalytic subunit Cgamma expressed and purified from sf9 cells.

The following PRKACG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKACG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu31969	NM_002732.3 Latest version!	Homo sapiens protein kinase cAMP-activated catalytic subunit gamma (PRKACG), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRKACG ( NM_002732.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002723.2 Homo sapiens protein kinase cAMP-activated catalytic subunit gamma (PRKACG), mRNA.

Price & Availability↑

CloneID	OHu31969
Clone ID Related Accession (Same CDS sequence)	NM_002732.3
Accession Version	NM_002732.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	cAMP-dependent protein kinase catalytic subunit gamma
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC039888.1 and AL162730.26. This sequence is a reference standard in the RefSeqGene project. On Sep 27, 2008 this sequence version replaced NM_002732.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC039888.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCAACG CCCCCGCCAA GAAGGACACC GAGCAGGAGG AGAGCGTGAA CGAGTTCCTA 
GCCAAAGCCA GAGGAGATTT CCTCTACAGA TGGGGAAACC CCGCTCAAAA CACCGCCAGC 
TCGGATCAGT TCGAACGGCT CAGGACGCTG GGCATGGGCT CCTTCGGGCG GGTGATGCTG 
GTGAGGCACC AGGAGACCGG CGGCCACTAC GCCATGAAGA TCCTCAACAA GCAGAAGGTG 
GTGAAGATGA AGCAGGTCGA GCACATACTG AACGAGAAGC GCATCCTGCA GGCGATCGAC 
TTTCCGTTCC TCGTCAAGCT CCAGTTCTCC TTTAAGGACA ACTCCTACCT GTACCTGGTG 
ATGGAGTACG TGCCGGGTGG GGAGATGTTC TCCCGCCTAC AGCGCGTCGG AAGGTTTAGC 
GAGCCCCATG CCTGTTTCTA TGCCGCCCAG GTCGTCCTGG CCGTCCAGTA CCTACACTCG 
CTCGACCTCA TCCACCGCGA CCTGAAGCCC GAGAATCTCC TCATCGACCA GCAGGGCTAC 
CTGCAGGTGA CGGACTTCGG TTTCGCCAAG CGCGTGAAGG GCCGCACTTG GACCTTGTGC 
GGGACCCCAG AGTACCTGGC CCCCGAGATC ATCCTGAGCA AAGGCTACAA CAAGGCCGTG 
GACTGGTGGG CCCTAGGGGT GCTCATCTAT GAGATGGCCG TGGGCTTCCC ACCCTTCTAC 
GCCGACCAGC CCATCCAGAT CTACGAGAAG ATCGTCTCTG GGAGGGTGCG GTTTCCCTCC 
AAACTCAGCT CTGACCTCAA GCATCTGCTG CGGAGCCTGC TGCAGGTGGA CCTCACCAAG 
CGCTTCGGAA ACCTCAGGAA CGGGGTTGGC GACATCAAGA ACCACAAGTG GTTCGCCACA 
ACCAGCTGGA TCGCCATCTA TGAGAAGAAG GTGGAAGCTC CCTTCATCCC GAAGTACACA 
GGCCCTGGGG ATGCCAGTAA CTTTGACGAC TACGAGGAGG AAGAGCTCCG GATCTCCATC 
AATGAGAAGT GTGCCAAGGA GTTTTCTGAG TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002723.2
CDS	32..1087
Translation	MGNAPAKKDTEQEESVNEFLAKARGDFLYRWGNPAQNTASSDQFERLRTLGMGSFGRVMLVRHQETGGHYAMKILNKQKVVKMKQVEHILNEKRILQAIDFPFLVKLQFSFKDNSYLYLVMEYVPGGEMFSRLQRVGRFSEPHACFYAAQVVLAVQYLHSLDLIHRDLKPENLLIDQQGYLQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAVGFPPFYADQPIQIYEKIVSGRVRFPSKLSSDLKHLLRSLLQVDLTKRFGNLRNGVGDIKNHKWFATTSWIAIYEKKVEAPFIPKYTGPGDASNFDDYEEEELRISINEKCAKEFSEF

Target ORF information:

RefSeq Version	NM_002732.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase cAMP-activated catalytic subunit gamma (PRKACG), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002732.3

ORF Insert Sequence:

ATGGGCAACG CCCCCGCCAA GAAGGACACC GAGCAGGAGG AGAGCGTGAA CGAGTTCCTA 
GCCAAAGCCA GAGGAGATTT CCTCTACAGA TGGGGAAACC CCGCTCAAAA CACCGCCAGC 
TCGGATCAGT TCGAACGGCT CAGGACGCTG GGCATGGGCT CCTTCGGGCG GGTGATGCTG 
GTGAGGCACC AGGAGACCGG CGGCCACTAC GCCATGAAGA TCCTCAACAA GCAGAAGGTG 
GTGAAGATGA AGCAGGTCGA GCACATACTG AACGAGAAGC GCATCCTGCA GGCGATCGAC 
TTTCCGTTCC TCGTCAAGCT CCAGTTCTCC TTTAAGGACA ACTCCTACCT GTACCTGGTG 
ATGGAGTACG TGCCGGGTGG GGAGATGTTC TCCCGCCTAC AGCGCGTCGG AAGGTTTAGC 
GAGCCCCATG CCTGTTTCTA TGCCGCCCAG GTCGTCCTGG CCGTCCAGTA CCTACACTCG 
CTCGACCTCA TCCACCGCGA CCTGAAGCCC GAGAATCTCC TCATCGACCA GCAGGGCTAC 
CTGCAGGTGA CGGACTTCGG TTTCGCCAAG CGCGTGAAGG GCCGCACTTG GACCTTGTGC 
GGGACCCCAG AGTACCTGGC CCCCGAGATC ATCCTGAGCA AAGGCTACAA CAAGGCCGTG 
GACTGGTGGG CCCTAGGGGT GCTCATCTAT GAGATGGCCG TGGGCTTCCC ACCCTTCTAC 
GCCGACCAGC CCATCCAGAT CTACGAGAAG ATCGTCTCTG GGAGGGTGCG GTTTCCCTCC 
AAACTCAGCT CTGACCTCAA GCATCTGCTG CGGAGCCTGC TGCAGGTGGA CCTCACCAAG 
CGCTTCGGAA ACCTCAGGAA CGGGGTTGGC GACATCAAGA ACCACAAGTG GTTCGCCACA 
ACCAGCTGGA TCGCCATCTA TGAGAAGAAG GTGGAAGCTC CCTTCATCCC GAAGTACACA 
GGCCCTGGGG ATGCCAGTAA CTTTGACGAC TACGAGGAGG AAGAGCTCCG GATCTCCATC 
AATGAGAAGT GTGCCAAGGA GTTTTCTGAG TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A new form of macrothrombocytopenia induced by a germ-line mutation in the PRKACG gene.
Blood124(16)2554-63(2014 Oct)
Manchev VT,Hilpert M,Berrou E,Elaib Z,Aouba A,Boukour S,Souquere S,Pierron G,Rameau P,Andrews R,Lanza F,Bobe R,Vainchenker W,Rosa JP,Bryckaert M,Debili N,Favier R,Raslova H

Overexpressing PKIB in prostate cancer promotes its aggressiveness by linking between PKA and Akt pathways.
Oncogene28(32)2849-59(2009 Aug)
Chung S,Furihata M,Tamura K,Uemura M,Daigo Y,Nasu Y,Miki T,Shuin T,Fujioka T,Nakamura Y,Nakagawa H

PKA-mediated stabilization of FoxH1 negatively regulates ERalpha activity.
Molecules and cells28(1)67-71(2009 Jul)
Yum J,Jeong HM,Kim S,Seo JW,Han Y,Lee KY,Yeo CY

Ser276 phosphorylation of NF-kB p65 by MSK1 controls SCF expression in inflammation.
PloS one4(2)e4393(2009)
Reber L,Vermeulen L,Haegeman G,Frossard N

Human immunodeficiency virus proteins induce the inhibitory cAMP/protein kinase A pathway in normal lymphocytes.
Proceedings of the National Academy of Sciences of the United States of America90(14)6676-80(1993 Jul)
Hofmann B,Nishanian P,Nguyen T,Insixiengmay P,Fahey JL

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PRKACG cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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