DDX27 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX27
Entrez Gene ID	55661
Full Name	DEAD-box helicase 27
Synonyms	DRS1,Drs1p,HSPC259,PP3241,RHLP,dJ686N3.1
General protein information	Preferred Names DEAD-box helicase 27 Names probable ATP-dependent RNA helicase DDX27 DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 DEAD box protein 27 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 27 Deficiency of ribosomal subunits protein 1 homolog RNA helicase-like protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20q13.13
Summary	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein involved in the processing of 5.8S and 28S ribosomal RNAs. More specifically, the encoded protein localizes to the nucleolus, where it interacts with the PeBoW complex to ensure proper 3' end formation of 47S rRNA. [provided by RefSeq, Jan 2017].
Disorder MIM:	616621

mRNA and Protein(s)

mRNA	Protein	Name
NM_001348187.1	NP_001335116.1	probable ATP-dependent RNA helicase DDX27 isoform 1
NM_017895.7	NP_060365.7	probable ATP-dependent RNA helicase DDX27 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	DDX27	NP_060365.7
Pan troglodytes (chimpanzee)	DDX27	XP_001166506.1
Danio rerio (zebrafish)	ddx27	NP_001002869.1
	DDX27	XP_001099400.1
Canis lupus familiaris (dog)	DDX27	XP_534451.2
Mus musculus (house mouse)	Ddx27	NP_694705.2
Gallus gallus (chicken)	DDX27	NP_001006293.1
Rattus norvegicus (Norway rat)	Ddx27	NP_001129273.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011084	XP_309566.4
Xenopus tropicalis (tropical clawed frog)	ddx27	XP_004919149.1
Bos taurus (cattle)	DDX27	NP_001073740.1
Drosophila melanogaster (fruit fly)	Rs1	NP_651970.1
Caenorhabditis elegans (roundworm)	Y71G12B.8	NP_490891.2

Gene Ontology
Bibliography

Related articles in PubMed

DEAD-box helicase DDX27 regulates 3' end formation of ribosomal 47S RNA and stably associates with the PeBoW-complex.
Kellner M, Rohrmoser M, Forn? I, Voss K, Burger K, M?hl B, Gruber-Eber A, Kremmer E, Imhof A, Eick D
Experimental cell research334(1)146-59(2015 May)

An integrative approach identified genes associated with drug response in gastric cancer.
Zhou J, Yong WP, Yap CS, Vijayaraghavan A, Sinha RA, Singh BK, Xiu S, Manesh S, Ngo A, Lim A, Ang C, Xie C, Wong FY, Lin SJ, Wan WK, Tan IB, Flotow H, Tan P, Lim KH, Yen PM, Goh LK
Carcinogenesis36(4)441-51(2015 Apr)

Expression of DDX27 contributes to colony-forming ability of gastric cancer cells and correlates with poor prognosis in gastric cancer.
Tsukamoto Y, Fumoto S, Noguchi T, Yanagihara K, Hirashita Y, Nakada C, Hijiya N, Uchida T, Matsuura K, Hamanaka R, Murakami K, Seto M, Inomata M, Moriyama M
American journal of cancer research5(10)2998-3014(2015)

The protein composition of mitotic chromosomes determined using multiclassifier combinatorial proteomics.
Ohta S, Bukowski-Wills JC, Sanchez-Pulido L, Alves Fde L, Wood L, Chen ZA, Platani M, Fischer L, Hudson DF, Ponting CP, Fukagawa T, Earnshaw WC, Rappsilber J
Cell142(5)810-21(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The DDX27 can interact specifically with the Pes1 and Bop1 but fulfils critical function(s) for proper 3' end formation of 47S rRNA independently of the PeBoW-complex.
Title: DEAD-box helicase DDX27 regulates 3' end formation of ribosomal 47S RNA and stably associates with the PeBoW-complex.

Levels of DDX27 mRNA and protein were increased in early-stage gastric tumors, and may be a potential diagnostic and prognostic marker for Gastric cancer.
Title: An integrative approach identified genes associated with drug response in gastric cancer.

The following DDX27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu31841	NM_017895.7 Latest version!	Homo sapiens DEAD-box helicase 27 (DDX27), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu69591	NM_001348187.1 Latest version!	Homo sapiens DEAD-box helicase 27 (DDX27), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX27 ( NM_017895.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_060365.7 Homo sapiens DEAD-box helicase 27 (DDX27), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu31841
Clone ID Related Accession (Same CDS sequence)	NM_017895.7
Accession Version	NM_017895.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX27 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB442175.2, BC126287.1 and BM970582.1. On Mar 6, 2009 this sequence version replaced NM_017895.6. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.232534.1, SRR1803615.123945.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTACTTG CGCAAAGACG ACGAGGAGGC TGCGAAAAGT TAAGGGCCGG ACCGCAGGCT 
GTGCTCGCTT CCGGAAGTGG CTTCTGCGAC AACATGCTTG CGGACCTCGG CTTAATCGGA 
ACCATAGGCG AGGATGACGA GGTGCCGGTG GAGCCCGAGT CTGACTCCGG GGACGAGGAA 
GAGGAGGGGC CCATTGTGCT GGGCAGACGA CAAAAAGCTT TGGGGAAGAA CCGCAGTGCT 
GATTTCAACC CTGATTTCGT TTTCACTGAG AAGGAGGGGA CGTACGATGG CAGCTGGGCC 
CTGGCTGATG TCATGAGCCA ACTCAAGAAG AAGAGGGCAG CCACTACATT AGATGAGAAG 
ATTGAGAAAG TTCGAAAGAA AAGGAAAACA GAGGATAAAG AAGCCAAGTC TGGGAAGTTG 
GAAAAGGAGA AAGAAGCAAA GGAAGGCTCT GAACCAAAGG AGCAGGAAGA CCTTCAAGAG 
AATGATGAGG AAGGCTCAGA AGATGAAGCC TCGGAGACTG ACTACTCATC AGCTGATGAG 
AACATCCTCA CCAAAGCAGA TACACTCAAA GTAAAGGATC GGAAGAAGAA GAAGAAGAAA 
GGACAGGAAG CAGGAGGATT TTTTGAAGAT GCATCTCAGT ACGATGAAAA CCTCTCGTTC 
CAGGACATGA ACCTTTCCCG CCCTCTTCTG AAGGCCATTA CAGCCATGGG CTTCAAGCAG 
CCCACCCCGA TCCAGAAGGC GTGCATACCT GTGGGTCTAT TGGGGAAGGA CATCTGTGCC 
TGTGCAGCCA CTGGGACAGG TAAAACTGCC GCCTTTGCCC TGCCTGTTTT GGAGCGTCTG 
ATTTATAAAC CCCGCCAGGC TCCAGTCACC CGCGTGCTGG TGCTAGTGCC CACCCGAGAG 
CTGGGCATCC AGGTGCACTC TGTCACCAGA CAGCTGGCCC AGTTCTGCAA CATCACCACC 
TGCCTGGCTG TGGGCGGCTT GGATGTGAAG TCTCAGGAAG CAGCTCTTCG GGCAGCGCCT 
GACATCCTCA TCGCCACCCC AGGCCGGCTC ATCGATCACC TCCACAACTG CCCTTCCTTC 
CACCTGAGCA GCATCGAGGT GCTCATCCTG GACGAGGCTG ACAGGATGCT GGATGAGTAC 
TTTGAGGAGC AGATGAAGGA GATCATCCGA ATGTGTTCCC ACCACCGCCA GACCATGCTC 
TTCTCGGCCA CCATGACAGA CGAGGTGAAA GATCTGGCTT CTGTCTCCTT GAAGAATCCT 
GTCCGGATAT TTGTGAACAG CAACACAGAT GTGGCTCCCT TCCTGCGGCA GGAGTTCATC 
CGGATCCGGC CTAATCGTGA AGGAGACCGG GAAGCCATCG TGGCAGCTTT GTTGACGAGG 
ACCTTCACTG ACCATGTGAT GCTGTTCACG CAAACCAAGA AGCAGGCCCA CCGCATGCAC 
ATCCTCCTGG GGCTCATGGG GCTGCAGGTG GGTGAGCTCC ATGGCAACTT GTCACAGACG 
CAGCGGCTGG AGGCCCTCCG GCGTTTTAAG GATGAACAGA TTGACATCCT CGTGGCCACT 
GATGTGGCAG CCCGTGGACT TGACATTGAG GGGGTCAAAA CGGTAATCAA CTTCACAATG 
CCTAATACCA TCAAACATTA TGTCCACCGG GTGGGGCGAA CAGCACGTGC TGGCAGGGCT 
GGGCGCTCAG TCTCTCTGGT GGGAGAAGAT GAGCGGAAGA TGCTGAAGGA GATTGTAAAA 
GCTGCCAAGG CCCCTGTGAA GGCCAGGATA CTTCCCCAAG ATGTCATCCT CAAATTCCGG 
GACAAGATTG AGAAAATGGA GAAAGATGTG TATGCAGTTC TGCAGCTAGA GGCGGAGGAA 
AAAGAGATGC AGCAGTCAGA AGCCCAGATC AATACAGCAA AGCGGCTCCT GGAGAAGGGG 
AAGGAGGCAG TGGTCCAAGA GCCCGAGAGG AGCTGGTTCC AGACCAAAGA AGAGAGGAAG 
AAGGAGAAAA TTGCCAAAGC TCTGCAGGAA TTTGACTTGG CCTTAAGAGG AAAGAAGAAA 
AGGAAGAAGT TTATGAAGGA TGCCAAAAAA AAGGGGGAGA TGACAGCAGA GGAAAGGTCT 
CAGTTTGAAA TCCTCAAGGC GCAGATGTTT GCTGAACGGC TAGCGAAGAG GAATCGCAGA 
GCCAAGCGGG CCCGAGCAAT GCCCGAGGAG GAGCCAGTGA GAGGTCCTGC CAAGAAGCAA 
AAGCAGGGGA AGAAATCTGT ATTTGATGAA GAACTCACCA ACACAAGCAA GAAGGCCCTG 
AAACAGTATC GAGCTGGCCC TTCCTTTGAA GAAAGGAAAC AGTTGGGCTT GCCCCACCAG 
AGACGAGGAG GAAACTTTAA ATCTAAATCC AGATACAAGA GGAGGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060365.7
CDS	62..2452
Translation	MVLAQRRRGGCEKLRAGPQAVLASGSGFCDNMLADLGLIGTIGEDDEVPVEPESDSGDEEEEGPIVLGRRQKALGKNRSADFNPDFVFTEKEGTYDGSWALADVMSQLKKKRAATTLDEKIEKVRKKRKTEDKEAKSGKLEKEKEAKEGSEPKEQEDLQENDEEGSEDEASETDYSSADENILTKADTLKVKDRKKKKKKGQEAGGFFEDASQYDENLSFQDMNLSRPLLKAITAMGFKQPTPIQKACIPVGLLGKDICACAATGTGKTAAFALPVLERLIYKPRQAPVTRVLVLVPTRELGIQVHSVTRQLAQFCNITTCLAVGGLDVKSQEAALRAAPDILIATPGRLIDHLHNCPSFHLSSIEVLILDEADRMLDEYFEEQMKEIIRMCSHHRQTMLFSATMTDEVKDLASVSLKNPVRIFVNSNTDVAPFLRQEFIRIRPNREGDREAIVAALLTRTFTDHVMLFTQTKKQAHRMHILLGLMGLQVGELHGNLSQTQRLEALRRFKDEQIDILVATDVAARGLDIEGVKTVINFTMPNTIKHYVHRVGRTARAGRAGRSVSLVGEDERKMLKEIVKAAKAPVKARILPQDVILKFRDKIEKMEKDVYAVLQLEAEEKEMQQSEAQINTAKRLLEKGKEAVVQEPERSWFQTKEERKKEKIAKALQEFDLALRGKKKRKKFMKDAKKKGEMTAEERSQFEILKAQMFAERLAKRNRRAKRARAMPEEEPVRGPAKKQKQGKKSVFDEELTNTSKKALKQYRAGPSFEERKQLGLPHQRRGGNFKSKSRYKRRK

Target ORF information:

RefSeq Version	NM_017895.7
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 27 (DDX27), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017895.7

ORF Insert Sequence:

ATGGTACTTG CGCAAAGACG ACGAGGAGGC TGCGAAAAGT TAAGGGCCGG ACCGCAGGCT 
GTGCTCGCTT CCGGAAGTGG CTTCTGCGAC AACATGCTTG CGGACCTCGG CTTAATCGGA 
ACCATAGGCG AGGATGACGA GGTGCCGGTG GAGCCCGAGT CTGACTCCGG GGACGAGGAA 
GAGGAGGGGC CCATTGTGCT GGGCAGACGA CAAAAAGCTT TGGGGAAGAA CCGCAGTGCT 
GATTTCAACC CTGATTTCGT TTTCACTGAG AAGGAGGGGA CGTACGATGG CAGCTGGGCC 
CTGGCTGATG TCATGAGCCA ACTCAAGAAG AAGAGGGCAG CCACTACATT AGATGAGAAG 
ATTGAGAAAG TTCGAAAGAA AAGGAAAACA GAGGATAAAG AAGCCAAGTC TGGGAAGTTG 
GAAAAGGAGA AAGAAGCAAA GGAAGGCTCT GAACCAAAGG AGCAGGAAGA CCTTCAAGAG 
AATGATGAGG AAGGCTCAGA AGATGAAGCC TCGGAGACTG ACTACTCATC AGCTGATGAG 
AACATCCTCA CCAAAGCAGA TACACTCAAA GTAAAGGATC GGAAGAAGAA GAAGAAGAAA 
GGACAGGAAG CAGGAGGATT TTTTGAAGAT GCATCTCAGT ACGATGAAAA CCTCTCGTTC 
CAGGACATGA ACCTTTCCCG CCCTCTTCTG AAGGCCATTA CAGCCATGGG CTTCAAGCAG 
CCCACCCCGA TCCAGAAGGC GTGCATACCT GTGGGTCTAT TGGGGAAGGA CATCTGTGCC 
TGTGCAGCCA CTGGGACAGG TAAAACTGCC GCCTTTGCCC TGCCTGTTTT GGAGCGTCTG 
ATTTATAAAC CCCGCCAGGC TCCAGTCACC CGCGTGCTGG TGCTAGTGCC CACCCGAGAG 
CTGGGCATCC AGGTGCACTC TGTCACCAGA CAGCTGGCCC AGTTCTGCAA CATCACCACC 
TGCCTGGCTG TGGGCGGCTT GGATGTGAAG TCTCAGGAAG CAGCTCTTCG GGCAGCGCCT 
GACATCCTCA TCGCCACCCC AGGCCGGCTC ATCGATCACC TCCACAACTG CCCTTCCTTC 
CACCTGAGCA GCATCGAGGT GCTCATCCTG GACGAGGCTG ACAGGATGCT GGATGAGTAC 
TTTGAGGAGC AGATGAAGGA GATCATCCGA ATGTGTTCCC ACCACCGCCA GACCATGCTC 
TTCTCGGCCA CCATGACAGA CGAGGTGAAA GATCTGGCTT CTGTCTCCTT GAAGAATCCT 
GTCCGGATAT TTGTGAACAG CAACACAGAT GTGGCTCCCT TCCTGCGGCA GGAGTTCATC 
CGGATCCGGC CTAATCGTGA AGGAGACCGG GAAGCCATCG TGGCAGCTTT GTTGACGAGG 
ACCTTCACTG ACCATGTGAT GCTGTTCACG CAAACCAAGA AGCAGGCCCA CCGCATGCAC 
ATCCTCCTGG GGCTCATGGG GCTGCAGGTG GGTGAGCTCC ATGGCAACTT GTCACAGACG 
CAGCGGCTGG AGGCCCTCCG GCGTTTTAAG GATGAACAGA TTGACATCCT CGTGGCCACT 
GATGTGGCAG CCCGTGGACT TGACATTGAG GGGGTCAAAA CGGTAATCAA CTTCACAATG 
CCTAATACCA TCAAACATTA TGTCCACCGG GTGGGGCGAA CAGCACGTGC TGGCAGGGCT 
GGGCGCTCAG TCTCTCTGGT GGGAGAAGAT GAGCGGAAGA TGCTGAAGGA GATTGTAAAA 
GCTGCCAAGG CCCCTGTGAA GGCCAGGATA CTTCCCCAAG ATGTCATCCT CAAATTCCGG 
GACAAGATTG AGAAAATGGA GAAAGATGTG TATGCAGTTC TGCAGCTAGA GGCGGAGGAA 
AAAGAGATGC AGCAGTCAGA AGCCCAGATC AATACAGCAA AGCGGCTCCT GGAGAAGGGG 
AAGGAGGCAG TGGTCCAAGA GCCCGAGAGG AGCTGGTTCC AGACCAAAGA AGAGAGGAAG 
AAGGAGAAAA TTGCCAAAGC TCTGCAGGAA TTTGACTTGG CCTTAAGAGG AAAGAAGAAA 
AGGAAGAAGT TTATGAAGGA TGCCAAAAAA AAGGGGGAGA TGACAGCAGA GGAAAGGTCT 
CAGTTTGAAA TCCTCAAGGC GCAGATGTTT GCTGAACGGC TAGCGAAGAG GAATCGCAGA 
GCCAAGCGGG CCCGAGCAAT GCCCGAGGAG GAGCCAGTGA GAGGTCCTGC CAAGAAGCAA 
AAGCAGGGGA AGAAATCTGT ATTTGATGAA GAACTCACCA ACACAAGCAA GAAGGCCCTG 
AAACAGTATC GAGCTGGCCC TTCCTTTGAA GAAAGGAAAC AGTTGGGCTT GCCCCACCAG 
AGACGAGGAG GAAACTTTAA ATCTAAATCC AGATACAAGA GGAGGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX27 ( NM_001348187.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335116.1 Homo sapiens DEAD-box helicase 27 (DDX27), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu69591
Clone ID Related Accession (Same CDS sequence)	NM_001348187.1
Accession Version	NM_001348187.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX27 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357560.11 and AL049766.14. On Feb 8, 2017 this sequence version replaced XM_011528888.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.253484.1, SRR1803611.48806.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTACTTG CGCAAAGACG ACGAGGAGGC TGCGAAAAGT TAAGGGCCGG ACCGCAGGCT 
GTGCTCGCTT CCGGAAGTGG CTTCTGCGAC AACATGCTTG CGGACCTCGG CTTAATCGGA 
ACCATAGGCG AGGATGACGA GGTGCCGGTG GAGCCCGAGT CTGACTCCGG GGACGAGGAA 
GAGGAGGGGC CCATTGTGCT GGGCAGACGA CAAAAAGCTT TGGGGAAGAA CCGCAGTGCT 
GATTTCAACC CTGATTTCGT TTTCACTGAG AAGGAGGGGA CGTACGATGG CAGCTGGGCC 
CTGGCTGATG TCATGAGCCA ACTCAAGAAG AAGAGGGCAG CCACTACATT AGATGAGAAG 
ATTGAGAAAG TTCGAAAGAA AAGGAAAACA GAGGATAAAG AAGCCAAGTC TGGGAAGTTG 
GAAAAGGAGA AAGAAGCAAA GGAAGGCTCT GAACCAAAGG AGCAGGAAGA CCTTCAAGAG 
AATGATGAGG AAGGCTCAGA AGATGAAGCC TCGGAGACTG ACTACTCATC AGCTGATGAG 
AACATCCTCA CCAAAGCAGA TACACTCAAA GTAAAGGATC GGAAGAAGAA GAAGAAGAAA 
GGACAGGAAG CAGGAGGATT TTTTGAAGAT GCATCTCAGT ACGATGAAAA CCTCTCGTTC 
CAGGACATGA ACCTTTCCCG CCCTCTTCTG AAGGCCATTA CAGCCATGGG CTTCAAGCAG 
CCCACCCCGA TCCAGAAGGC GTGCATACCT GTGGGTCTAT TGGGGAAGGA CATCTGTGCC 
TGTGCAGCCA CTGGGACAGG TAAAACTGCC GCCTTTGCCC TGCCTGTTTT GGAGCGTCTG 
ATTTATAAAC CCCGCCAGGC TCCAGTCACC CGCGTGCTGG TGCTAGTGCC CACCCGAGAG 
CTGGGCATCC AGGTGCACTC TGTCACCAGA CAGCTGGCCC AGTTCTGCAA CATCACCACC 
TGCCTGGCTG TGGGCGGCTT GGATGTGAAG TCTCAGGAAG CAGCTCTTCG GGCAGCGCCT 
GACATCCTCA TCGCCACCCC AGGCCGGCTC ATCGATCACC TCCACAACTG CCCTTCCTTC 
CACCTGAGCA GCATCGAGGT GCTCATCCTG GACGAGGCTG ACAGCCCGCC TGTCGACACA 
GCCTGGCCAC ACGCCCCCAC TTTGTGGCTT CTGCATTTGC CATTTCCTCA GCCTGGAGCC 
ATCTCCTGCC CCTTCAGGAT GCTGGATGAG TACTTTGAGG AGCAGATGAA GGAGATCATC 
CGAATGTGTT CCCACCACCG CCAGACCATG CTCTTCTCGG CCACCATGAC AGACGAGGTG 
AAAGATCTGG CTTCTGTCTC CTTGAAGAAT CCTGTCCGGA TATTTGTGAA CAGCAACACA 
GATGTGGCTC CCTTCCTGCG GCAGGAGTTC ATCCGGATCC GGCCTAATCG TGAAGGAGAC 
CGGGAAGCCA TCGTGGCAGC TTTGTTGACG AGGACCTTCA CTGACCATGT GATGCTGTTC 
ACGCAAACCA AGAAGCAGGC CCACCGCATG CACATCCTCC TGGGGCTCAT GGGGCTGCAG 
GTGGGTGAGC TCCATGGCAA CTTGTCACAG ACGCAGCGGC TGGAGGCCCT CCGGCGTTTT 
AAGGATGAAC AGATTGACAT CCTCGTGGCC ACTGATGTGG CAGCCCGTGG ACTTGACATT 
GAGGGGGTCA AAACGGTAAT CAACTTCACA ATGCCTAATA CCATCAAACA TTATGTCCAC 
CGGGTGGGGC GAACAGCACG TGCTGGCAGG GCTGGGCGCT CAGTCTCTCT GGTGGGAGAA 
GATGAGCGGA AGATGCTGAA GGAGATTGTA AAAGCTGCCA AGGCCCCTGT GAAGGCCAGG 
ATACTTCCCC AAGATGTCAT CCTCAAATTC CGGGACAAGA TTGAGAAAAT GGAGAAAGAT 
GTGTATGCAG TTCTGCAGCT AGAGGCGGAG GAAAAAGAGA TGCAGCAGTC AGAAGCCCAG 
ATCAATACAG CAAAGCGGCT CCTGGAGAAG GGGAAGGAGG CAGTGGTCCA AGAGCCCGAG 
AGGAGCTGGT TCCAGACCAA AGAAGAGAGG AAGAAGGAGA AAATTGCCAA AGCTCTGCAG 
GAATTTGACT TGGCCTTAAG AGGAAAGAAG AAAAGGAAGA AGTTTATGAA GGATGCCAAA 
AAAAAGGGGG AGATGACAGC AGAGGAAAGG TCTCAGTTTG AAATCCTCAA GGCGCAGATG 
TTTGCTGAAC GGCTAGCGAA GAGGAATCGC AGAGCCAAGC GGGCCCGAGC AATGCCCGAG 
GAGGAGCCAG TGAGAGGTCC TGCCAAGAAG CAAAAGCAGG GGAAGAAATC TGTATTTGAT 
GAAGAACTCA CCAACACAAG CAAGAAGGCC CTGAAACAGT ATCGAGCTGG CCCTTCCTTT 
GAAGAAAGGA AACAGTTGGG CTTGCCCCAC CAGAGACGAG GAGGAAACTT TAAATCTAAA 
TCCAGATACA AGAGGAGGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335116.1
CDS	62..2545
Translation	MVLAQRRRGGCEKLRAGPQAVLASGSGFCDNMLADLGLIGTIGEDDEVPVEPESDSGDEEEEGPIVLGRRQKALGKNRSADFNPDFVFTEKEGTYDGSWALADVMSQLKKKRAATTLDEKIEKVRKKRKTEDKEAKSGKLEKEKEAKEGSEPKEQEDLQENDEEGSEDEASETDYSSADENILTKADTLKVKDRKKKKKKGQEAGGFFEDASQYDENLSFQDMNLSRPLLKAITAMGFKQPTPIQKACIPVGLLGKDICACAATGTGKTAAFALPVLERLIYKPRQAPVTRVLVLVPTRELGIQVHSVTRQLAQFCNITTCLAVGGLDVKSQEAALRAAPDILIATPGRLIDHLHNCPSFHLSSIEVLILDEADSPPVDTAWPHAPTLWLLHLPFPQPGAISCPFRMLDEYFEEQMKEIIRMCSHHRQTMLFSATMTDEVKDLASVSLKNPVRIFVNSNTDVAPFLRQEFIRIRPNREGDREAIVAALLTRTFTDHVMLFTQTKKQAHRMHILLGLMGLQVGELHGNLSQTQRLEALRRFKDEQIDILVATDVAARGLDIEGVKTVINFTMPNTIKHYVHRVGRTARAGRAGRSVSLVGEDERKMLKEIVKAAKAPVKARILPQDVILKFRDKIEKMEKDVYAVLQLEAEEKEMQQSEAQINTAKRLLEKGKEAVVQEPERSWFQTKEERKKEKIAKALQEFDLALRGKKKRKKFMKDAKKKGEMTAEERSQFEILKAQMFAERLAKRNRRAKRARAMPEEEPVRGPAKKQKQGKKSVFDEELTNTSKKALKQYRAGPSFEERKQLGLPHQRRGGNFKSKSRYKRRK

Target ORF information:

RefSeq Version	NM_001348187.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 27 (DDX27), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348187.1

ORF Insert Sequence:

ATGGTACTTG CGCAAAGACG ACGAGGAGGC TGCGAAAAGT TAAGGGCCGG ACCGCAGGCT 
GTGCTCGCTT CCGGAAGTGG CTTCTGCGAC AACATGCTTG CGGACCTCGG CTTAATCGGA 
ACCATAGGCG AGGATGACGA GGTGCCGGTG GAGCCCGAGT CTGACTCCGG GGACGAGGAA 
GAGGAGGGGC CCATTGTGCT GGGCAGACGA CAAAAAGCTT TGGGGAAGAA CCGCAGTGCT 
GATTTCAACC CTGATTTCGT TTTCACTGAG AAGGAGGGGA CGTACGATGG CAGCTGGGCC 
CTGGCTGATG TCATGAGCCA ACTCAAGAAG AAGAGGGCAG CCACTACATT AGATGAGAAG 
ATTGAGAAAG TTCGAAAGAA AAGGAAAACA GAGGATAAAG AAGCCAAGTC TGGGAAGTTG 
GAAAAGGAGA AAGAAGCAAA GGAAGGCTCT GAACCAAAGG AGCAGGAAGA CCTTCAAGAG 
AATGATGAGG AAGGCTCAGA AGATGAAGCC TCGGAGACTG ACTACTCATC AGCTGATGAG 
AACATCCTCA CCAAAGCAGA TACACTCAAA GTAAAGGATC GGAAGAAGAA GAAGAAGAAA 
GGACAGGAAG CAGGAGGATT TTTTGAAGAT GCATCTCAGT ACGATGAAAA CCTCTCGTTC 
CAGGACATGA ACCTTTCCCG CCCTCTTCTG AAGGCCATTA CAGCCATGGG CTTCAAGCAG 
CCCACCCCGA TCCAGAAGGC GTGCATACCT GTGGGTCTAT TGGGGAAGGA CATCTGTGCC 
TGTGCAGCCA CTGGGACAGG TAAAACTGCC GCCTTTGCCC TGCCTGTTTT GGAGCGTCTG 
ATTTATAAAC CCCGCCAGGC TCCAGTCACC CGCGTGCTGG TGCTAGTGCC CACCCGAGAG 
CTGGGCATCC AGGTGCACTC TGTCACCAGA CAGCTGGCCC AGTTCTGCAA CATCACCACC 
TGCCTGGCTG TGGGCGGCTT GGATGTGAAG TCTCAGGAAG CAGCTCTTCG GGCAGCGCCT 
GACATCCTCA TCGCCACCCC AGGCCGGCTC ATCGATCACC TCCACAACTG CCCTTCCTTC 
CACCTGAGCA GCATCGAGGT GCTCATCCTG GACGAGGCTG ACAGCCCGCC TGTCGACACA 
GCCTGGCCAC ACGCCCCCAC TTTGTGGCTT CTGCATTTGC CATTTCCTCA GCCTGGAGCC 
ATCTCCTGCC CCTTCAGGAT GCTGGATGAG TACTTTGAGG AGCAGATGAA GGAGATCATC 
CGAATGTGTT CCCACCACCG CCAGACCATG CTCTTCTCGG CCACCATGAC AGACGAGGTG 
AAAGATCTGG CTTCTGTCTC CTTGAAGAAT CCTGTCCGGA TATTTGTGAA CAGCAACACA 
GATGTGGCTC CCTTCCTGCG GCAGGAGTTC ATCCGGATCC GGCCTAATCG TGAAGGAGAC 
CGGGAAGCCA TCGTGGCAGC TTTGTTGACG AGGACCTTCA CTGACCATGT GATGCTGTTC 
ACGCAAACCA AGAAGCAGGC CCACCGCATG CACATCCTCC TGGGGCTCAT GGGGCTGCAG 
GTGGGTGAGC TCCATGGCAA CTTGTCACAG ACGCAGCGGC TGGAGGCCCT CCGGCGTTTT 
AAGGATGAAC AGATTGACAT CCTCGTGGCC ACTGATGTGG CAGCCCGTGG ACTTGACATT 
GAGGGGGTCA AAACGGTAAT CAACTTCACA ATGCCTAATA CCATCAAACA TTATGTCCAC 
CGGGTGGGGC GAACAGCACG TGCTGGCAGG GCTGGGCGCT CAGTCTCTCT GGTGGGAGAA 
GATGAGCGGA AGATGCTGAA GGAGATTGTA AAAGCTGCCA AGGCCCCTGT GAAGGCCAGG 
ATACTTCCCC AAGATGTCAT CCTCAAATTC CGGGACAAGA TTGAGAAAAT GGAGAAAGAT 
GTGTATGCAG TTCTGCAGCT AGAGGCGGAG GAAAAAGAGA TGCAGCAGTC AGAAGCCCAG 
ATCAATACAG CAAAGCGGCT CCTGGAGAAG GGGAAGGAGG CAGTGGTCCA AGAGCCCGAG 
AGGAGCTGGT TCCAGACCAA AGAAGAGAGG AAGAAGGAGA AAATTGCCAA AGCTCTGCAG 
GAATTTGACT TGGCCTTAAG AGGAAAGAAG AAAAGGAAGA AGTTTATGAA GGATGCCAAA 
AAAAAGGGGG AGATGACAGC AGAGGAAAGG TCTCAGTTTG AAATCCTCAA GGCGCAGATG 
TTTGCTGAAC GGCTAGCGAA GAGGAATCGC AGAGCCAAGC GGGCCCGAGC AATGCCCGAG 
GAGGAGCCAG TGAGAGGTCC TGCCAAGAAG CAAAAGCAGG GGAAGAAATC TGTATTTGAT 
GAAGAACTCA CCAACACAAG CAAGAAGGCC CTGAAACAGT ATCGAGCTGG CCCTTCCTTT 
GAAGAAAGGA AACAGTTGGG CTTGCCCCAC CAGAGACGAG GAGGAAACTT TAAATCTAAA 
TCCAGATACA AGAGGAGGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DEAD-box helicase DDX27 regulates 3' end formation of ribosomal 47S RNA and stably associates with the PeBoW-complex.
Experimental cell research334(1)146-59(2015 May)
Kellner M,Rohrmoser M,Forn? I,Voss K,Burger K,M?hl B,Gruber-Eber A,Kremmer E,Imhof A,Eick D

An integrative approach identified genes associated with drug response in gastric cancer.
Carcinogenesis36(4)441-51(2015 Apr)
Zhou J,Yong WP,Yap CS,Vijayaraghavan A,Sinha RA,Singh BK,Xiu S,Manesh S,Ngo A,Lim A,Ang C,Xie C,Wong FY,Lin SJ,Wan WK,Tan IB,Flotow H,Tan P,Lim KH,Yen PM,Goh LK

Expression of DDX27 contributes to colony-forming ability of gastric cancer cells and correlates with poor prognosis in gastric cancer.
American journal of cancer research5(10)2998-3014(2015)
Tsukamoto Y,Fumoto S,Noguchi T,Yanagihara K,Hirashita Y,Nakada C,Hijiya N,Uchida T,Matsuura K,Hamanaka R,Murakami K,Seto M,Inomata M,Moriyama M

The protein composition of mitotic chromosomes determined using multiclassifier combinatorial proteomics.
Cell142(5)810-21(2010 Sep)
Ohta S,Bukowski-Wills JC,Sanchez-Pulido L,Alves Fde L,Wood L,Chen ZA,Platani M,Fischer L,Hudson DF,Ponting CP,Fukagawa T,Earnshaw WC,Rappsilber J

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DDX27 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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