NHP2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NHP2
Entrez Gene ID	55651
Full Name	NHP2 ribonucleoprotein
Synonyms	DKCB2,NHP2P,NOLA2
General protein information	Preferred Names NHP2 ribonucleoprotein Names H/ACA ribonucleoprotein complex subunit 2 NHP2 ribonucleoprotein homolog NHP2-like protein nucleolar protein family A, member 2 (H/ACA small nucleolar RNPs) snoRNP protein NHP2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q35.3
Summary	This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA1 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. The four H/ACA snoRNP proteins are also components of the telomerase complex. This gene encodes a protein related to Saccharomyces cerevisiae Nhp2p. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008].
Disorder MIM:	606470
Disorder Html:	Dyskeratosis congenita, autosomal recessive 2, 613987 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_017838.4	NP_060308.1	H/ACA ribonucleoprotein complex subunit 2 isoform a
NM_017838.3	NP_060308.1	H/ACA ribonucleoprotein complex subunit 2 isoform a
NM_001034833.1	NP_001030005.1	H/ACA ribonucleoprotein complex subunit 2 isoform b

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NHP2	XP_531874.1
Rattus norvegicus (Norway rat)	LOC100910210	XP_003750866.1
Gallus gallus (chicken)	NHP2	NP_001239073.1
Oryza sativa (rice)	Os02g0728600	NP_001048001.1
Kluyveromyces lactis	KLLA0C15345g	XP_452888.1
Mus musculus (house mouse)	Nhp2	NP_080907.1
Schizosaccharomyces pombe (fission yeast)	nhp2	NP_594717.1
Oryza sativa (rice)	Os06g0274200	NP_001057374.1
Homo sapiens (human)	NHP2	NP_060308.1
Danio rerio (zebrafish)	nhp2	NP_997762.1
Drosophila melanogaster (fruit fly)	NHP2	NP_001261849.1
Arabidopsis thaliana (thale cress)	AT5G08180	NP_196435.1
Xenopus tropicalis (tropical clawed frog)	nhp2	NP_988989.1
Pan troglodytes (chimpanzee)	NHP2	XP_003950736.1
	NHP2	XP_001095587.1
Bos taurus (cattle)	NHP2	NP_001015626.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012204	XP_320327.2
Caenorhabditis elegans (roundworm)	Y48A6B.3	NP_499415.1
Saccharomyces cerevisiae (baker's yeast)	NHP2	NP_010073.2
Eremothecium gossypii	AGOS_ABL115W	NP_982832.1

Gene Ontology
Bibliography

Related articles in PubMed

Control of box C/D snoRNP assembly by N
Huang L, Ashraf S, Wang J, Lilley DM
EMBO reports18(9)1631-1645(2017 Sep)

Widespread macromolecular interaction perturbations in human genetic disorders.
Sahni N, Yi S, Taipale M, Fuxman Bass JI, Coulombe-Huntington J, Yang F, Peng J, Weile J, Karras GI, Wang Y, Kov?cs IA, Kamburov A, Krykbaeva I, Lam MH, Tucker G, Khurana V, Sharma A, Liu YY, Yachie N, Zhong Q, Shen Y, Palagi A, San-Miguel A, Fan C, Balcha D, Dricot A, Jordan DM, Walsh JM, Shah AA, Yang X, Stoyanova AK, Leighton A, Calderwood MA, Jacob Y, Cusick ME, Salehi-Ashtiani K, Whitesell LJ, Sunyaev S, Berger B, Barab?si AL, Charloteaux B, Hill DE, Hao T, Roth FP, Xia Y, Walhout AJM, Lindquist S, Vidal M
Cell161(3)647-660(2015 Apr)

Detection of mutations in TERT, the genes for telomerase reverse transcriptase, in Indian patients of aplastic anaemia: a pilot study.
Mehta S, Krishnamohan M, Gulati S, Sharmat N, Vashishtha P, Singh I
The Journal of the Association of Physicians of India62(10)13-7(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Indian aplastic anemia patients did not have NHP2 mutations.
Title: Detection of mutations in TERT, the genes for telomerase reverse transcriptase, in Indian patients of aplastic anaemia: a pilot study.

Effects of dyskeratosis congenita mutations in NHP2 on assembly of H/ACA pre-RNPs
Title: Effects of dyskeratosis congenita mutations in dyskerin, NHP2 and NOP10 on assembly of H/ACA pre-RNPs.

The analysis of two other proteins, NHP2 and GAR1, that together with dyskerin and NOP10 are key components of telomerase and small nucleolar ribonucleoprotein (snoRNP) complexes, is described.
Title: Mutations in the telomerase component NHP2 cause the premature ageing syndrome dyskeratosis congenita.

Observational study of gene-disease association. (HuGE Navigator)
Title: Mutations in TERT, the gene for telomerase reverse transcriptase, in aplastic anemia.

NOLA2 expression in patients with squamous cell lung cancer increased by 70% which makes it a highly informative marker of squamous cell lung cancer
Title: [The NOLA2 and RPS3A genes as highly informative markers for human squamous cell lung cancer].

The following NHP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NHP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19298	NM_017838.4 Latest version!	Homo sapiens NHP2 ribonucleoprotein (NHP2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu16183	NM_001034833.1 Latest version!	Homo sapiens NHP2 ribonucleoprotein (NHP2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu19298	NM_017838.3	Homo sapiens NHP2 ribonucleoprotein (NHP2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NHP2 ( NM_017838.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060308.1 Homo sapiens NHP2 ribonucleoprotein (NHP2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19298
Clone ID Related Accession (Same CDS sequence)	NM_017838.4 , NM_017838.3
Accession Version	NM_017838.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	H/ACA ribonucleoprotein complex subunit 2 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE797477.1, BC000009.2 and AJ293309.1. On Dec 4, 2019 this sequence version replaced NM_017838.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC006387.2, AK000486.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACCAAAA TAAAGGCAGA TCCCGACGGG CCCGAGGCTC AGGCGGAGGC GTGTTCCGGG 
GAGCGCACCT ACCAGGAGCT GCTGGTCAAC CAGAACCCCA TCGCGCAGCC CCTGGCTTCT 
CGCCGCCTCA CGCGGAAGCT CTACAAATGC ATCAAGAAAG CGGTGAAGCA GAAGCAGATT 
CGGCGCGGGG TGAAAGAGGT TCAGAAATTT GTCAACAAAG GAGAAAAAGG GATCATGGTT 
TTGGCAGGAG ACACACTGCC CATTGAGGTA TACTGCCATC TCCCAGTCAT GTGTGAGGAC 
CGAAATTTGC CCTATGTCTA TATCCCCTCT AAGACGGACC TGGGTGCAGC CGCAGGCTCC 
AAGCGCCCCA CCTGTGTGAT AATGGTCAAG CCCCATGAGG AGTACCAGGA GGCTTACGAT 
GAGTGCCTGG AGGAGGTGCA GTCCCTGCCC CTACCCCTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060308.1
CDS	69..530
Translation	MTKIKADPDGPEAQAEACSGERTYQELLVNQNPIAQPLASRRLTRKLYKCIKKAVKQKQIRRGVKEVQKFVNKGEKGIMVLAGDTLPIEVYCHLPVMCEDRNLPYVYIPSKTDLGAAAGSKRPTCVIMVKPHEEYQEAYDECLEEVQSLPLPL

Target ORF information:

RefSeq Version	NM_017838.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NHP2 ribonucleoprotein (NHP2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017838.4

ORF Insert Sequence:

ATGACCAAAA TAAAGGCAGA TCCCGACGGG CCCGAGGCTC AGGCGGAGGC GTGTTCCGGG 
GAGCGCACCT ACCAGGAGCT GCTGGTCAAC CAGAACCCCA TCGCGCAGCC CCTGGCTTCT 
CGCCGCCTCA CGCGGAAGCT CTACAAATGC ATCAAGAAAG CGGTGAAGCA GAAGCAGATT 
CGGCGCGGGG TGAAAGAGGT TCAGAAATTT GTCAACAAAG GAGAAAAAGG GATCATGGTT 
TTGGCAGGAG ACACACTGCC CATTGAGGTA TACTGCCATC TCCCAGTCAT GTGTGAGGAC 
CGAAATTTGC CCTATGTCTA TATCCCCTCT AAGACGGACC TGGGTGCAGC CGCAGGCTCC 
AAGCGCCCCA CCTGTGTGAT AATGGTCAAG CCCCATGAGG AGTACCAGGA GGCTTACGAT 
GAGTGCCTGG AGGAGGTGCA GTCCCTGCCC CTACCCCTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHP2 ( NM_017838.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060308.1 Homo sapiens NHP2 ribonucleoprotein (NHP2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19298
Clone ID Related Accession (Same CDS sequence)	NM_017838.4 , NM_017838.3
Accession Version	NM_017838.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	H/ACA ribonucleoprotein complex subunit 2 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE797477.1, BC000009.2 and AJ293309.1. This sequence is a reference standard in the RefSeqGene project. On Oct 1, 2004 this sequence version replaced NM_017838.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.309785.1, SRR1163657.253008.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACCAAAA TAAAGGCAGA TCCCGACGGG CCCGAGGCTC AGGCGGAGGC GTGTTCCGGG 
GAGCGCACCT ACCAGGAGCT GCTGGTCAAC CAGAACCCCA TCGCGCAGCC CCTGGCTTCT 
CGCCGCCTCA CGCGGAAGCT CTACAAATGC ATCAAGAAAG CGGTGAAGCA GAAGCAGATT 
CGGCGCGGGG TGAAAGAGGT TCAGAAATTT GTCAACAAAG GAGAAAAAGG GATCATGGTT 
TTGGCAGGAG ACACACTGCC CATTGAGGTA TACTGCCATC TCCCAGTCAT GTGTGAGGAC 
CGAAATTTGC CCTATGTCTA TATCCCCTCT AAGACGGACC TGGGTGCAGC CGCAGGCTCC 
AAGCGCCCCA CCTGTGTGAT AATGGTCAAG CCCCATGAGG AGTACCAGGA GGCTTACGAT 
GAGTGCCTGG AGGAGGTGCA GTCCCTGCCC CTACCCCTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060308.1
CDS	144..605
Translation	MTKIKADPDGPEAQAEACSGERTYQELLVNQNPIAQPLASRRLTRKLYKCIKKAVKQKQIRRGVKEVQKFVNKGEKGIMVLAGDTLPIEVYCHLPVMCEDRNLPYVYIPSKTDLGAAAGSKRPTCVIMVKPHEEYQEAYDECLEEVQSLPLPL

Target ORF information:

RefSeq Version	NM_017838.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NHP2 ribonucleoprotein (NHP2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017838.3

ORF Insert Sequence:

ATGACCAAAA TAAAGGCAGA TCCCGACGGG CCCGAGGCTC AGGCGGAGGC GTGTTCCGGG 
GAGCGCACCT ACCAGGAGCT GCTGGTCAAC CAGAACCCCA TCGCGCAGCC CCTGGCTTCT 
CGCCGCCTCA CGCGGAAGCT CTACAAATGC ATCAAGAAAG CGGTGAAGCA GAAGCAGATT 
CGGCGCGGGG TGAAAGAGGT TCAGAAATTT GTCAACAAAG GAGAAAAAGG GATCATGGTT 
TTGGCAGGAG ACACACTGCC CATTGAGGTA TACTGCCATC TCCCAGTCAT GTGTGAGGAC 
CGAAATTTGC CCTATGTCTA TATCCCCTCT AAGACGGACC TGGGTGCAGC CGCAGGCTCC 
AAGCGCCCCA CCTGTGTGAT AATGGTCAAG CCCCATGAGG AGTACCAGGA GGCTTACGAT 
GAGTGCCTGG AGGAGGTGCA GTCCCTGCCC CTACCCCTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHP2 ( NM_001034833.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001030005.1 Homo sapiens NHP2 ribonucleoprotein (NHP2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16183
Clone ID Related Accession (Same CDS sequence)	NM_001034833.1
Accession Version	NM_001034833.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Homo sapiens(human)
Product	H/ACA ribonucleoprotein complex subunit 2 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG827491.1, BM913344.1 and AJ293309.1. Transcript Variant: This variant (2) lacks an alternate coding exon compared to variant 1, that causes a frameshift. The resulting protein (isoform b) is shorter and has a distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR995652.1, BM913344.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCAAAA TAAAGGCAGA TCCCGACGGG CCCGAGGCTC AGGCGGAGGC GTGTTCCGGG 
GAGCGCACCT ACCAGGAGCT GCTGGTCAAC CAGAACCCCA TCGCGCAGCC CCTGGCTTCT 
CGCCGCCTCA CGCGGAAGCT CTACAAATGC ATCAAGAAAG CGGTGAAGCA GAAGCAGATT 
CGGCGCGGGG TGAAAGAGGT TCAGAAATTT GTCAACAAAG GAGAAAAAGG GACCTGGGTG 
CAGCCGCAGG CTCCAAGCGC CCCACCTGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001030005.1
CDS	144..416
Translation	MTKIKADPDGPEAQAEACSGERTYQELLVNQNPIAQPLASRRLTRKLYKCIKKAVKQKQIRRGVKEVQKFVNKGEKGTWVQPQAPSAPPV

Target ORF information:

RefSeq Version	NM_001034833.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NHP2 ribonucleoprotein (NHP2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034833.1

ORF Insert Sequence:

ATGACCAAAA TAAAGGCAGA TCCCGACGGG CCCGAGGCTC AGGCGGAGGC GTGTTCCGGG 
GAGCGCACCT ACCAGGAGCT GCTGGTCAAC CAGAACCCCA TCGCGCAGCC CCTGGCTTCT 
CGCCGCCTCA CGCGGAAGCT CTACAAATGC ATCAAGAAAG CGGTGAAGCA GAAGCAGATT 
CGGCGCGGGG TGAAAGAGGT TCAGAAATTT GTCAACAAAG GAGAAAAAGG GACCTGGGTG 
CAGCCGCAGG CTCCAAGCGC CCCACCTGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Control of box C/D snoRNP assembly by N
EMBO reports18(9)1631-1645(2017 Sep)
Huang L,Ashraf S,Wang J,Lilley DM

Widespread macromolecular interaction perturbations in human genetic disorders.
Cell161(3)647-660(2015 Apr)
Sahni N,Yi S,Taipale M,Fuxman Bass JI,Coulombe-Huntington J,Yang F,Peng J,Weile J,Karras GI,Wang Y,Kov?cs IA,Kamburov A,Krykbaeva I,Lam MH,Tucker G,Khurana V,Sharma A,Liu YY,Yachie N,Zhong Q,Shen Y,Palagi A,San-Miguel A,Fan C,Balcha D,Dricot A,Jordan DM,Walsh JM,Shah AA,Yang X,Stoyanova AK,Leighton A,Calderwood MA,Jacob Y,Cusick ME,Salehi-Ashtiani K,Whitesell LJ,Sunyaev S,Berger B,Barab?si AL,Charloteaux B,Hill DE,Hao T,Roth FP,Xia Y,Walhout AJM,Lindquist S,Vidal M

Detection of mutations in TERT, the genes for telomerase reverse transcriptase, in Indian patients of aplastic anaemia: a pilot study.
The Journal of the Association of Physicians of India62(10)13-7(2014 Oct)
Mehta S,Krishnamohan M,Gulati S,Sharmat N,Vashishtha P,Singh I

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NHP2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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