PRKAB1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRKAB1
Entrez Gene ID	5564
Full Name	protein kinase AMP-activated non-catalytic subunit beta 1
Synonyms	AMPK,HAMPKb
General protein information	Preferred Names protein kinase AMP-activated non-catalytic subunit beta 1 Names 5'-AMP-activated protein kinase subunit beta-1 5'-AMP-activated protein kinase beta-1 subunit AMP-activated protein kinase beta subunit AMPK beta -1 chain AMPK beta 1 AMPK subunit beta-1 AMPKb protein kinase, AMP-activated, beta 1 non-catalytic subunit protein kinase, AMP-activated, noncatalytic, beta-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.23
Summary	The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq, Jul 2008].
Disorder MIM:	602740

mRNA and Protein(s)

mRNA	Protein	Name
XM_005253909.1	XP_005253966.1	5'-AMP-activated protein kinase subunit beta-1 isoform X1
NM_006253.4	NP_006244.2	5'-AMP-activated protein kinase subunit beta-1
NM_006253.5	NP_006244.2	5'-AMP-activated protein kinase subunit beta-1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PRKAB1	NP_001035001.1
Schizosaccharomyces pombe (fission yeast)	amk2	NP_588485.1
	PRKAB1	XP_001085323.1
Bos taurus (cattle)	PRKAB1	NP_001019729.1
Canis lupus familiaris (dog)	PRKAB1	XP_543421.2
Danio rerio (zebrafish)	prkab1a	NP_001002632.1
Drosophila melanogaster (fruit fly)	alc	NP_610460.1
Mus musculus (house mouse)	Prkab1	NP_114075.1
Rattus norvegicus (Norway rat)	Prkab1	NP_114182.1
Magnaporthe oryzae (rice blast fungus)	MGG_06930	XP_003709669.1
Arabidopsis thaliana (thale cress)	AT4G16360	NP_001031650.2
Xenopus tropicalis (tropical clawed frog)	prkab1	XP_002932123.1
Homo sapiens (human)	PRKAB1	NP_006244.2
Pan troglodytes (chimpanzee)	PRKAB1	NP_001233530.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008195	XP_317273.3
Neurospora crassa	NCU03837	XP_960596.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

WIPI3 and WIPI4 ?-propellers are scaffolds for LKB1-AMPK-TSC signalling circuits in the control of autophagy.
Bakula D, M?ller AJ, Zuleger T, Takacs Z, Franz-Wachtel M, Thost AK, Brigger D, Tschan MP, Frickey T, Robenek H, Macek B, Proikas-Cezanne T
Nature communications815637(2017 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we studied the role of the beta 1 subunit in erythrocytes and observed microcytic anemia with compensatory extramedullary hematopoiesis together with splenomegaly and increased osmotic resistance.
Title: The AMP-activated protein kinase beta 1 subunit modulates erythrocyte integrity.

Salicylate activates the AMP-activated protein kinase (AMPK) by binding at the A-769662 drug binding site on the AMPK beta1-subunit
Title: Salicylate activates AMPK and synergizes with metformin to reduce the survival of prostate and lung cancer cells ex vivo through inhibition of de novo lipogenesis.

findings suggest that the reduced expression of AMPK-beta1 confers lower AMPK activity, which enhances the oncogenic capacity of advanced-stage ovarian cancer.
Title: Reduced expression of AMPK-?1 during tumor progression enhances the oncogenic capacity of advanced ovarian cancer.

LKB1 controls IRS1-dependent adipogenesis via AMPK in white adipose tissue.
Title: Liver kinase b1 is required for white adipose tissue growth and differentiation.

Data indicate that a diet high in iron improves glucose tolerance by activating AMPK through mechanisms that include deacetylation.
Title: Iron regulates glucose homeostasis in liver and muscle via AMP-activated protein kinase in mice.

The following PRKAB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKAB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02113	NM_006253.5 Latest version!	Homo sapiens protein kinase AMP-activated non-catalytic subunit beta 1 (PRKAB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu02113	XM_005253909.1 Latest version!	Homo sapiens protein kinase AMP-activated non-catalytic subunit beta 1 (PRKAB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu02113	NM_006253.4	Homo sapiens protein kinase AMP-activated non-catalytic subunit beta 1 (PRKAB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRKAB1 ( NM_006253.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006244.2 Homo sapiens protein kinase AMP-activated non-catalytic subunit beta 1 (PRKAB1), mRNA.

Price & Availability↑

CloneID	OHu02113
Clone ID Related Accession (Same CDS sequence)	XM_005253909.1 , NM_006253.4 , NM_006253.5
Accession Version	NM_006253.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	5'-AMP-activated protein kinase subunit beta-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU539177.1, BC017671.1 and AF022116.1. On Oct 29, 2003 this sequence version replaced NM_006253.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF022116.1, SRR1163655.468227.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAATA CCAGCAGTGA GCGCGCCGCG CTGGAGCGGC ATGGTGGCCA TAAGACGCCC 
CGGAGGGACA GCTCGGGGGG CACCAAGGAC GGGGACAGGC CCAAGATCCT GATGGACAGC 
CCCGAAGACG CCGACCTCTT CCACTCCGAG GAAATCAAGG CACCAGAGAA GGAGGAATTC 
CTGGCCTGGC AGCATGATCT GGAAGTGAAT GATAAAGCTC CCGCCCAGGC TCGGCCAACG 
GTGTTTCGAT GGACGGGGGG CGGAAAGGAA GTTTACTTAT CTGGGTCCTT CAACAACTGG 
AGTAAACTTC CCCTCACCAG AAGCCACAAT AACTTTGTAG CCATCCTGGA TCTGCCGGAA 
GGAGAGCATC AGTACAAGTT CTTTGTGGAT GGTCAGTGGA CGCACGACCC TTCCGAGCCC 
ATAGTAACCA GCCAGCTTGG CACAGTTAAC AACATCATTC AAGTGAAGAA AACTGACTTT 
GAAGTATTTG ATGCTTTAAT GGTGGATTCC CAAAAGTGCT CCGATGTGTC TGAGCTGTCC 
AGTTCTCCCC CAGGACCCTA CCATCAGGAG CCCTACGTCT GCAAACCCGA AGAGCGCTTT 
CGGGCACCCC CTATTCTCCC CCCACATCTC CTCCAGGTCA TCCTGAACAA GGACACGGGG 
ATTTCCTGTG ATCCAGCTTT GCTTCCTGAG CCCAATCACG TCATGCTGAA CCACCTATAC 
GCGCTGTCTA TCAAGGATGG AGTGATGGTG CTCAGCGCAA CCCACCGGTA CAAGAAGAAG 
TACGTCACCA CCTTGTTATA CAAGCCCATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006244.2
CDS	290..1102
Translation	MGNTSSERAALERHGGHKTPRRDSSGGTKDGDRPKILMDSPEDADLFHSEEIKAPEKEEFLAWQHDLEVNDKAPAQARPTVFRWTGGGKEVYLSGSFNNWSKLPLTRSHNNFVAILDLPEGEHQYKFFVDGQWTHDPSEPIVTSQLGTVNNIIQVKKTDFEVFDALMVDSQKCSDVSELSSSPPGPYHQEPYVCKPEERFRAPPILPPHLLQVILNKDTGISCDPALLPEPNHVMLNHLYALSIKDGVMVLSATHRYKKKYVTTLLYKPI

Target ORF information:

RefSeq Version	NM_006253.4
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase AMP-activated non-catalytic subunit beta 1 (PRKAB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006253.4

ORF Insert Sequence:

ATGGGCAATA CCAGCAGTGA GCGCGCCGCG CTGGAGCGGC ATGGTGGCCA TAAGACGCCC 
CGGAGGGACA GCTCGGGGGG CACCAAGGAC GGGGACAGGC CCAAGATCCT GATGGACAGC 
CCCGAAGACG CCGACCTCTT CCACTCCGAG GAAATCAAGG CACCAGAGAA GGAGGAATTC 
CTGGCCTGGC AGCATGATCT GGAAGTGAAT GATAAAGCTC CCGCCCAGGC TCGGCCAACG 
GTGTTTCGAT GGACGGGGGG CGGAAAGGAA GTTTACTTAT CTGGGTCCTT CAACAACTGG 
AGTAAACTTC CCCTCACCAG AAGCCACAAT AACTTTGTAG CCATCCTGGA TCTGCCGGAA 
GGAGAGCATC AGTACAAGTT CTTTGTGGAT GGTCAGTGGA CGCACGACCC TTCCGAGCCC 
ATAGTAACCA GCCAGCTTGG CACAGTTAAC AACATCATTC AAGTGAAGAA AACTGACTTT 
GAAGTATTTG ATGCTTTAAT GGTGGATTCC CAAAAGTGCT CCGATGTGTC TGAGCTGTCC 
AGTTCTCCCC CAGGACCCTA CCATCAGGAG CCCTACGTCT GCAAACCCGA AGAGCGCTTT 
CGGGCACCCC CTATTCTCCC CCCACATCTC CTCCAGGTCA TCCTGAACAA GGACACGGGG 
ATTTCCTGTG ATCCAGCTTT GCTTCCTGAG CCCAATCACG TCATGCTGAA CCACCTATAC 
GCGCTGTCTA TCAAGGATGG AGTGATGGTG CTCAGCGCAA CCCACCGGTA CAAGAAGAAG 
TACGTCACCA CCTTGTTATA CAAGCCCATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKAB1 ( NM_006253.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_006244.2 Homo sapiens protein kinase AMP-activated non-catalytic subunit beta 1 (PRKAB1), mRNA.

Price & Availability↑

CloneID	OHu02113
Clone ID Related Accession (Same CDS sequence)	XM_005253909.1 , NM_006253.4 , NM_006253.5
Accession Version	NM_006253.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-13
Organism	Homo sapiens(human)
Product	5'-AMP-activated protein kinase subunit beta-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU539177.1, BC017671.1 and AF022116.1. On Nov 22, 2018 this sequence version replaced NM_006253.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC017671.1, AF022116.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000229328.10/ ENSP00000229328.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCAATA CCAGCAGTGA GCGCGCCGCG CTGGAGCGGC ATGGTGGCCA TAAGACGCCC 
CGGAGGGACA GCTCGGGGGG CACCAAGGAC GGGGACAGGC CCAAGATCCT GATGGACAGC 
CCCGAAGACG CCGACCTCTT CCACTCCGAG GAAATCAAGG CACCAGAGAA GGAGGAATTC 
CTGGCCTGGC AGCATGATCT GGAAGTGAAT GATAAAGCTC CCGCCCAGGC TCGGCCAACG 
GTGTTTCGAT GGACGGGGGG CGGAAAGGAA GTTTACTTAT CTGGGTCCTT CAACAACTGG 
AGTAAACTTC CCCTCACCAG AAGCCACAAT AACTTTGTAG CCATCCTGGA TCTGCCGGAA 
GGAGAGCATC AGTACAAGTT CTTTGTGGAT GGTCAGTGGA CGCACGACCC TTCCGAGCCC 
ATAGTAACCA GCCAGCTTGG CACAGTTAAC AACATCATTC AAGTGAAGAA AACTGACTTT 
GAAGTATTTG ATGCTTTAAT GGTGGATTCC CAAAAGTGCT CCGATGTGTC TGAGCTGTCC 
AGTTCTCCCC CAGGACCCTA CCATCAGGAG CCCTACGTCT GCAAACCCGA AGAGCGCTTT 
CGGGCACCCC CTATTCTCCC CCCACATCTC CTCCAGGTCA TCCTGAACAA GGACACGGGG 
ATTTCCTGTG ATCCAGCTTT GCTTCCTGAG CCCAATCACG TCATGCTGAA CCACCTATAC 
GCGCTGTCTA TCAAGGATGG AGTGATGGTG CTCAGCGCAA CCCACCGGTA CAAGAAGAAG 
TACGTCACCA CCTTGTTATA CAAGCCCATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006244.2
CDS	113..925
Translation	MGNTSSERAALERHGGHKTPRRDSSGGTKDGDRPKILMDSPEDADLFHSEEIKAPEKEEFLAWQHDLEVNDKAPAQARPTVFRWTGGGKEVYLSGSFNNWSKLPLTRSHNNFVAILDLPEGEHQYKFFVDGQWTHDPSEPIVTSQLGTVNNIIQVKKTDFEVFDALMVDSQKCSDVSELSSSPPGPYHQEPYVCKPEERFRAPPILPPHLLQVILNKDTGISCDPALLPEPNHVMLNHLYALSIKDGVMVLSATHRYKKKYVTTLLYKPI

Target ORF information:

RefSeq Version	NM_006253.5
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase AMP-activated non-catalytic subunit beta 1 (PRKAB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006253.5

ORF Insert Sequence:

ATGGGCAATA CCAGCAGTGA GCGCGCCGCG CTGGAGCGGC ATGGTGGCCA TAAGACGCCC 
CGGAGGGACA GCTCGGGGGG CACCAAGGAC GGGGACAGGC CCAAGATCCT GATGGACAGC 
CCCGAAGACG CCGACCTCTT CCACTCCGAG GAAATCAAGG CACCAGAGAA GGAGGAATTC 
CTGGCCTGGC AGCATGATCT GGAAGTGAAT GATAAAGCTC CCGCCCAGGC TCGGCCAACG 
GTGTTTCGAT GGACGGGGGG CGGAAAGGAA GTTTACTTAT CTGGGTCCTT CAACAACTGG 
AGTAAACTTC CCCTCACCAG AAGCCACAAT AACTTTGTAG CCATCCTGGA TCTGCCGGAA 
GGAGAGCATC AGTACAAGTT CTTTGTGGAT GGTCAGTGGA CGCACGACCC TTCCGAGCCC 
ATAGTAACCA GCCAGCTTGG CACAGTTAAC AACATCATTC AAGTGAAGAA AACTGACTTT 
GAAGTATTTG ATGCTTTAAT GGTGGATTCC CAAAAGTGCT CCGATGTGTC TGAGCTGTCC 
AGTTCTCCCC CAGGACCCTA CCATCAGGAG CCCTACGTCT GCAAACCCGA AGAGCGCTTT 
CGGGCACCCC CTATTCTCCC CCCACATCTC CTCCAGGTCA TCCTGAACAA GGACACGGGG 
ATTTCCTGTG ATCCAGCTTT GCTTCCTGAG CCCAATCACG TCATGCTGAA CCACCTATAC 
GCGCTGTCTA TCAAGGATGG AGTGATGGTG CTCAGCGCAA CCCACCGGTA CAAGAAGAAG 
TACGTCACCA CCTTGTTATA CAAGCCCATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKAB1 ( XM_005253909.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005253966.1 Homo sapiens protein kinase AMP-activated non-catalytic subunit beta 1 (PRKAB1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu02113
Clone ID Related Accession (Same CDS sequence)	XM_005253909.1 , NM_006253.4 , NM_006253.5
Accession Version	XM_005253909.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	5'-AMP-activated protein kinase subunit beta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATA CCAGCAGTGA GCGCGCCGCG CTGGAGCGGC ATGGTGGCCA TAAGACGCCC 
CGGAGGGACA GCTCGGGGGG CACCAAGGAC GGGGACAGGC CCAAGATCCT GATGGACAGC 
CCCGAAGACG CCGACCTCTT CCACTCCGAG GAAATCAAGG CACCAGAGAA GGAGGAATTC 
CTGGCCTGGC AGCATGATCT GGAAGTGAAT GATAAAGCTC CCGCCCAGGC TCGGCCAACG 
GTGTTTCGAT GGACGGGGGG CGGAAAGGAA GTTTACTTAT CTGGGTCCTT CAACAACTGG 
AGTAAACTTC CCCTCACCAG AAGCCACAAT AACTTTGTAG CCATCCTGGA TCTGCCGGAA 
GGAGAGCATC AGTACAAGTT CTTTGTGGAT GGTCAGTGGA CGCACGACCC TTCCGAGCCC 
ATAGTAACCA GCCAGCTTGG CACAGTTAAC AACATCATTC AAGTGAAGAA AACTGACTTT 
GAAGTATTTG ATGCTTTAAT GGTGGATTCC CAAAAGTGCT CCGATGTGTC TGAGCTGTCC 
AGTTCTCCCC CAGGACCCTA CCATCAGGAG CCCTACGTCT GCAAACCCGA AGAGCGCTTT 
CGGGCACCCC CTATTCTCCC CCCACATCTC CTCCAGGTCA TCCTGAACAA GGACACGGGG 
ATTTCCTGTG ATCCAGCTTT GCTTCCTGAG CCCAATCACG TCATGCTGAA CCACCTATAC 
GCGCTGTCTA TCAAGGATGG AGTGATGGTG CTCAGCGCAA CCCACCGGTA CAAGAAGAAG 
TACGTCACCA CCTTGTTATA CAAGCCCATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005253966.1
CDS	192..1004
Translation	MGNTSSERAALERHGGHKTPRRDSSGGTKDGDRPKILMDSPEDADLFHSEEIKAPEKEEFLAWQHDLEVNDKAPAQARPTVFRWTGGGKEVYLSGSFNNWSKLPLTRSHNNFVAILDLPEGEHQYKFFVDGQWTHDPSEPIVTSQLGTVNNIIQVKKTDFEVFDALMVDSQKCSDVSELSSSPPGPYHQEPYVCKPEERFRAPPILPPHLLQVILNKDTGISCDPALLPEPNHVMLNHLYALSIKDGVMVLSATHRYKKKYVTTLLYKPI

Target ORF information:

RefSeq Version	XM_005253909.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase AMP-activated non-catalytic subunit beta 1 (PRKAB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253909.1

ORF Insert Sequence:

ATGGGCAATA CCAGCAGTGA GCGCGCCGCG CTGGAGCGGC ATGGTGGCCA TAAGACGCCC 
CGGAGGGACA GCTCGGGGGG CACCAAGGAC GGGGACAGGC CCAAGATCCT GATGGACAGC 
CCCGAAGACG CCGACCTCTT CCACTCCGAG GAAATCAAGG CACCAGAGAA GGAGGAATTC 
CTGGCCTGGC AGCATGATCT GGAAGTGAAT GATAAAGCTC CCGCCCAGGC TCGGCCAACG 
GTGTTTCGAT GGACGGGGGG CGGAAAGGAA GTTTACTTAT CTGGGTCCTT CAACAACTGG 
AGTAAACTTC CCCTCACCAG AAGCCACAAT AACTTTGTAG CCATCCTGGA TCTGCCGGAA 
GGAGAGCATC AGTACAAGTT CTTTGTGGAT GGTCAGTGGA CGCACGACCC TTCCGAGCCC 
ATAGTAACCA GCCAGCTTGG CACAGTTAAC AACATCATTC AAGTGAAGAA AACTGACTTT 
GAAGTATTTG ATGCTTTAAT GGTGGATTCC CAAAAGTGCT CCGATGTGTC TGAGCTGTCC 
AGTTCTCCCC CAGGACCCTA CCATCAGGAG CCCTACGTCT GCAAACCCGA AGAGCGCTTT 
CGGGCACCCC CTATTCTCCC CCCACATCTC CTCCAGGTCA TCCTGAACAA GGACACGGGG 
ATTTCCTGTG ATCCAGCTTT GCTTCCTGAG CCCAATCACG TCATGCTGAA CCACCTATAC 
GCGCTGTCTA TCAAGGATGG AGTGATGGTG CTCAGCGCAA CCCACCGGTA CAAGAAGAAG 
TACGTCACCA CCTTGTTATA CAAGCCCATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

WIPI3 and WIPI4 ?-propellers are scaffolds for LKB1-AMPK-TSC signalling circuits in the control of autophagy.
Nature communications815637(2017 May)
Bakula D,M?ller AJ,Zuleger T,Takacs Z,Franz-Wachtel M,Thost AK,Brigger D,Tschan MP,Frickey T,Robenek H,Macek B,Proikas-Cezanne T

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PRKAB1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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