PRKAA2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRKAA2
Entrez Gene ID	5563
Full Name	protein kinase AMP-activated catalytic subunit alpha 2
Synonyms	AMPK,AMPK2,AMPKa2,PRKAA
General protein information	Preferred Names protein kinase AMP-activated catalytic subunit alpha 2 Names 5'-AMP-activated protein kinase catalytic subunit alpha-2 5'-AMP-activated protein kinase, catalytic alpha-2 chain ACACA kinase AMP-activated protein kinase alpha-2 subunit variant 2 AMP-activated protein kinase alpha-2 subunit variant 3 AMPK alpha 2 AMPK subunit alpha-2 AMPK-alpha-2 chain HMGCR kinase acetyl-CoA carboxylase kinase hydroxymethylglutaryl-CoA reductase kinase protein kinase, AMP-activated, alpha 2 catalytic subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p32.2
Summary	The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq, Jul 2008].
Disorder MIM:	600497

mRNA and Protein(s)

mRNA	Protein	Name
NM_006252.3	NP_006243.2	5'-AMP-activated protein kinase catalytic subunit alpha-2
NM_006252.4	NP_006243.2	5'-AMP-activated protein kinase catalytic subunit alpha-2
XM_017001693.1	XP_016857182.1	5'-AMP-activated protein kinase catalytic subunit alpha-2 isoform X1
XM_017001694.1	XP_016857183.1	5'-AMP-activated protein kinase catalytic subunit alpha-2 isoform X1
XM_017001692.1	XP_016857181.1	5'-AMP-activated protein kinase catalytic subunit alpha-2 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PRKAA2	XP_001144587.1
Mus musculus (house mouse)	Prkaa2	NP_835279.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002686	XP_312237.4
Caenorhabditis elegans (roundworm)	aak-2	NP_510710.2
Neurospora crassa	NCU04566	XP_958665.2
	PRKAA2	XP_002801614.1
Bos taurus (cattle)	PRKAA2	NP_001192534.1
Rattus norvegicus (Norway rat)	Prkaa2	NP_076481.1
Danio rerio (zebrafish)	prkaa2	XP_700831.4
Drosophila melanogaster (fruit fly)	SNF1A	NP_477313.1
Saccharomyces cerevisiae (baker's yeast)	SNF1	NP_010765.3
Schizosaccharomyces pombe (fission yeast)	ssp2	NP_588376.1
Kluyveromyces lactis	KLLA0A03806g	XP_451166.1
Oryza sativa (rice)	Os03g0289100	NP_001049790.1
Canis lupus familiaris (dog)	PRKAA2	XP_005620393.1
Gallus gallus (chicken)	PRKAA2	NP_001034694.1
Eremothecium gossypii	AGOS_AEL230W	NP_984631.1
Oryza sativa (rice)	Os08g0484600	NP_001062083.1
Homo sapiens (human)	PRKAA2	NP_006243.2
Magnaporthe oryzae (rice blast fungus)	MGG_00803	XP_003718181.1
Xenopus tropicalis (tropical clawed frog)	prkaa2	NP_001135554.1

Gene Ontology
Bibliography

Related articles in PubMed

AMPK-AKT double negative feedback loop in breast cancer cells regulates their adaptation to matrix deprivation.
Saha M, Kumar S, Bukhari S, Balaji SA, Kumar P, Hindupur SK, Rangarajan A
Cancer research(2018 Jan)

High-intensity high-volume swimming induces more robust signaling through PGC-1? and AMPK activation than sprint interval swimming in m. triceps brachii.
Casuso RA, Plaza-D?az J, Ruiz-Ojeda FJ, Arag?n-Vela J, Robles-Sanchez C, Nordsborg NB, Hebberecht M, Salmeron LM, Huertas JR
PloS one12(10)e0185494(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TNF-alpha treatment of colonic rho(0) cells augmented IL-8 expression by 9-fold (P < 0.01) via NF-kappaB compared to TNF-alpha-treated control. Moreover, reduced mitochondrial function facilitated TNF-alpha-mediated NF-kappaB luciferase promoter activity as a result of lowered inhibitory IkappaBalpha (nuclear factor of kappa light polypeptide gene enhancer in B-cell inhibitor, alpha), leading to elevated NF-kappaB. ...
Title: Reduced mitochondrial activity in colonocytes facilitates AMPK?2-dependent inflammation.

PRKAA deletion promoted mitochondrial fragmentation in vascular endothelial cells by inhibiting the autophagy-dependent degradation of DNM1L.
Title: Deletion of PRKAA triggers mitochondrial fission by inhibiting the autophagy-dependent degradation of DNM1L.

inactivation of AMPKalpha2, but not AMPKalpha1, abrogates the tumor attenuation caused by UBE2O loss.
Title: A UBE2O-AMPK?2 Axis that Promotes Tumor Initiation and Progression Offers Opportunities for Therapy.

AMPK phosphorylates DNMT1, RBBP7, and HAT1 and increases interactions of DNMT1, RBBP7, and HAT1.
Title: AMPK promotes mitochondrial biogenesis and function by phosphorylating the epigenetic factors DNMT1, RBBP7, and HAT1.

PGC-1alpha protein was higher after HIHVT than after SIT (p < 0.05). Moreover, the AMPKpTHR172/AMPK ratio increased at post after SIT (p < 0.05), whereas this effect was delayed after HIHVT as it increased after 3 h
Title: High-intensity high-volume swimming induces more robust signaling through PGC-1? and AMPK activation than sprint interval swimming in m. triceps brachii.

The following PRKAA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKAA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14987	NM_006252.4 Latest version!	Homo sapiens protein kinase AMP-activated catalytic subunit alpha 2 (PRKAA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu90199	XM_017001692.1 Latest version!	Homo sapiens protein kinase AMP-activated catalytic subunit alpha 2 (PRKAA2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu90199	XM_017001693.1 Latest version!	Homo sapiens protein kinase AMP-activated catalytic subunit alpha 2 (PRKAA2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu90199	XM_017001694.1 Latest version!	Homo sapiens protein kinase AMP-activated catalytic subunit alpha 2 (PRKAA2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu14987	NM_006252.3	Homo sapiens protein kinase AMP-activated catalytic subunit alpha 2 (PRKAA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRKAA2 ( NM_006252.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006243.2 Homo sapiens protein kinase AMP-activated catalytic subunit alpha 2 (PRKAA2), mRNA.

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CloneID	OHu14987
Clone ID Related Accession (Same CDS sequence)	NM_006252.3 , NM_006252.4
Accession Version	NM_006252.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	5'-AMP-activated protein kinase catalytic subunit alpha-2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA710012.1, BC069740.1, AL035705.22 and BM979925.1. On Oct 5, 2007 this sequence version replaced NM_006252.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because transcript sequence consistent with the reference genome assembly was not available for all regions of the RefSeq transcript. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK314011.1, SRR1803616.173171.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGAGA AGCAGAAGCA CGACGGGCGG GTGAAGATCG GACACTACGT GCTGGGCGAC 
ACGCTGGGCG TCGGCACCTT CGGCAAAGTG AAGATTGGAG AACATCAATT AACAGGCCAT 
AAAGTGGCAG TTAAAATCTT AAATAGACAG AAGATTCGCA GTTTAGATGT TGTTGGAAAA 
ATAAAACGAG AAATTCAAAA TCTAAAACTC TTTCGTCATC CTCATATTAT CAAACTATAC 
CAGGTGATCA GCACTCCAAC AGATTTTTTT ATGGTAATGG AATATGTGTC TGGAGGTGAA 
TTATTTGACT ACATCTGTAA GCATGGACGG GTTGAAGAGA TGGAAGCCAG GCGGCTCTTT 
CAGCAGATTC TGTCTGCTGT GGATTACTGT CATAGGCATA TGGTTGTTCA TCGAGACCTG 
AAACCAGAGA ATGTCCTGTT GGATGCACAC ATGAATGCCA AGATAGCCGA TTTCGGATTA 
TCTAATATGA TGTCAGATGG TGAATTTCTG AGAACTAGTT GCGGATCTCC AAATTATGCA 
GCACCTGAAG TCATCTCAGG CAGATTGTAT GCAGGTCCTG AAGTTGATAT CTGGAGCTGT 
GGTGTTATCT TGTATGCTCT TCTTTGTGGC ACCCTCCCAT TTGATGATGA GCATGTACCT 
ACGTTATTTA AGAAGATCCG AGGGGGTGTC TTTTATATCC CAGAATATCT CAATCGTTCT 
GTCGCCACTC TCCTGATGCA TATGCTGCAG GTTGACCCAC TGAAACGAGC AACTATCAAA 
GACATAAGAG AGCATGAATG GTTTAAACAA GATTTGCCCA GTTACTTATT TCCTGAAGAC 
CCTTCCTATG ATGCTAACGT CATTGATGAT GAGGCTGTGA AAGAAGTGTG TGAAAAATTT 
GAATGTACAG AATCAGAAGT AATGAACAGT TTATATAGTG GTGACCCTCA AGACCAGCTT 
GCAGTGGCTT ATCATCTTAT CATTGACAAT CGGAGAATAA TGAACCAAGC CAGTGAGTTC 
TACCTCGCCT CTAGTCCTCC ATCTGGTTCT TTTATGGATG ATAGTGCCAT GCATATTCCC 
CCAGGCCTGA AACCTCATCC AGAAAGGATG CCACCTCTTA TAGCAGACAG CCCCAAAGCA 
AGATGTCCAT TGGATGCACT GAATACGACT AAGCCCAAAT CTTTAGCTGT GAAAAAAGCC 
AAGTGGCATC TTGGAATCCG AAGTCAGAGC AAACCGTATG ACATTATGGC TGAAGTTTAC 
CGAGCTATGA AGCAGCTGGA TTTTGAATGG AAGGTAGTGA ATGCATACCA TCTTCGTGTA 
AGAAGAAAAA ATCCAGTGAC TGGCAATTAC GTGAAAATGA GCTTACAACT TTACCTGGTT 
GATAACAGGA GCTATCTTTT GGACTTTAAA AGCATTGATG ATGAAGTAGT GGAGCAGAGA 
TCTGGTTCCT CAACACCTCA GCGTTCCTGT TCTGCTGCTG GCTTACACAG ACCAAGATCA 
AGTTTTGATT CCACAACTGC AGAGAGCCAT TCACTTTCTG GCTCTCTCAC TGGCTCTTTG 
ACCGGAAGCA CATTGTCTTC AGTTTCACCT CGCCTGGGCA GTCACACCAT GGATTTTTTT 
GAAATGTGTG CCAGTCTGAT TACTACTTTA GCCCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006243.2
CDS	72..1730
Translation	MAEKQKHDGRVKIGHYVLGDTLGVGTFGKVKIGEHQLTGHKVAVKILNRQKIRSLDVVGKIKREIQNLKLFRHPHIIKLYQVISTPTDFFMVMEYVSGGELFDYICKHGRVEEMEARRLFQQILSAVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSCGVILYALLCGTLPFDDEHVPTLFKKIRGGVFYIPEYLNRSVATLLMHMLQVDPLKRATIKDIREHEWFKQDLPSYLFPEDPSYDANVIDDEAVKEVCEKFECTESEVMNSLYSGDPQDQLAVAYHLIIDNRRIMNQASEFYLASSPPSGSFMDDSAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKPKSLAVKKAKWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVVNAYHLRVRRKNPVTGNYVKMSLQLYLVDNRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRPRSSFDSTTAESHSLSGSLTGSLTGSTLSSVSPRLGSHTMDFFEMCASLITTLAR

Target ORF information:

RefSeq Version	NM_006252.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase AMP-activated catalytic subunit alpha 2 (PRKAA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006252.3

ORF Insert Sequence:

ATGGCTGAGA AGCAGAAGCA CGACGGGCGG GTGAAGATCG GACACTACGT GCTGGGCGAC 
ACGCTGGGCG TCGGCACCTT CGGCAAAGTG AAGATTGGAG AACATCAATT AACAGGCCAT 
AAAGTGGCAG TTAAAATCTT AAATAGACAG AAGATTCGCA GTTTAGATGT TGTTGGAAAA 
ATAAAACGAG AAATTCAAAA TCTAAAACTC TTTCGTCATC CTCATATTAT CAAACTATAC 
CAGGTGATCA GCACTCCAAC AGATTTTTTT ATGGTAATGG AATATGTGTC TGGAGGTGAA 
TTATTTGACT ACATCTGTAA GCATGGACGG GTTGAAGAGA TGGAAGCCAG GCGGCTCTTT 
CAGCAGATTC TGTCTGCTGT GGATTACTGT CATAGGCATA TGGTTGTTCA TCGAGACCTG 
AAACCAGAGA ATGTCCTGTT GGATGCACAC ATGAATGCCA AGATAGCCGA TTTCGGATTA 
TCTAATATGA TGTCAGATGG TGAATTTCTG AGAACTAGTT GCGGATCTCC AAATTATGCA 
GCACCTGAAG TCATCTCAGG CAGATTGTAT GCAGGTCCTG AAGTTGATAT CTGGAGCTGT 
GGTGTTATCT TGTATGCTCT TCTTTGTGGC ACCCTCCCAT TTGATGATGA GCATGTACCT 
ACGTTATTTA AGAAGATCCG AGGGGGTGTC TTTTATATCC CAGAATATCT CAATCGTTCT 
GTCGCCACTC TCCTGATGCA TATGCTGCAG GTTGACCCAC TGAAACGAGC AACTATCAAA 
GACATAAGAG AGCATGAATG GTTTAAACAA GATTTGCCCA GTTACTTATT TCCTGAAGAC 
CCTTCCTATG ATGCTAACGT CATTGATGAT GAGGCTGTGA AAGAAGTGTG TGAAAAATTT 
GAATGTACAG AATCAGAAGT AATGAACAGT TTATATAGTG GTGACCCTCA AGACCAGCTT 
GCAGTGGCTT ATCATCTTAT CATTGACAAT CGGAGAATAA TGAACCAAGC CAGTGAGTTC 
TACCTCGCCT CTAGTCCTCC ATCTGGTTCT TTTATGGATG ATAGTGCCAT GCATATTCCC 
CCAGGCCTGA AACCTCATCC AGAAAGGATG CCACCTCTTA TAGCAGACAG CCCCAAAGCA 
AGATGTCCAT TGGATGCACT GAATACGACT AAGCCCAAAT CTTTAGCTGT GAAAAAAGCC 
AAGTGGCATC TTGGAATCCG AAGTCAGAGC AAACCGTATG ACATTATGGC TGAAGTTTAC 
CGAGCTATGA AGCAGCTGGA TTTTGAATGG AAGGTAGTGA ATGCATACCA TCTTCGTGTA 
AGAAGAAAAA ATCCAGTGAC TGGCAATTAC GTGAAAATGA GCTTACAACT TTACCTGGTT 
GATAACAGGA GCTATCTTTT GGACTTTAAA AGCATTGATG ATGAAGTAGT GGAGCAGAGA 
TCTGGTTCCT CAACACCTCA GCGTTCCTGT TCTGCTGCTG GCTTACACAG ACCAAGATCA 
AGTTTTGATT CCACAACTGC AGAGAGCCAT TCACTTTCTG GCTCTCTCAC TGGCTCTTTG 
ACCGGAAGCA CATTGTCTTC AGTTTCACCT CGCCTGGGCA GTCACACCAT GGATTTTTTT 
GAAATGTGTG CCAGTCTGAT TACTACTTTA GCCCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKAA2 ( NM_006252.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006243.2 Homo sapiens protein kinase AMP-activated catalytic subunit alpha 2 (PRKAA2), mRNA.

Price & Availability↑

CloneID	OHu14987
Clone ID Related Accession (Same CDS sequence)	NM_006252.3 , NM_006252.4
Accession Version	NM_006252.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	5'-AMP-activated protein kinase catalytic subunit alpha-2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC099789.2, DA710012.1, BC069740.1, AL035705.22 and BM979925.1. On Nov 22, 2018 this sequence version replaced NM_006252.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because transcript sequence consistent with the reference genome assembly was not available for all regions of the RefSeq transcript. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.173171.1, SRR1803616.12773.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000371244.9/ ENSP00000360290.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGAGA AGCAGAAGCA CGACGGGCGG GTGAAGATCG GACACTACGT GCTGGGCGAC 
ACGCTGGGCG TCGGCACCTT CGGCAAAGTG AAGATTGGAG AACATCAATT AACAGGCCAT 
AAAGTGGCAG TTAAAATCTT AAATAGACAG AAGATTCGCA GTTTAGATGT TGTTGGAAAA 
ATAAAACGAG AAATTCAAAA TCTAAAACTC TTTCGTCATC CTCATATTAT CAAACTATAC 
CAGGTGATCA GCACTCCAAC AGATTTTTTT ATGGTAATGG AATATGTGTC TGGAGGTGAA 
TTATTTGACT ACATCTGTAA GCATGGACGG GTTGAAGAGA TGGAAGCCAG GCGGCTCTTT 
CAGCAGATTC TGTCTGCTGT GGATTACTGT CATAGGCATA TGGTTGTTCA TCGAGACCTG 
AAACCAGAGA ATGTCCTGTT GGATGCACAC ATGAATGCCA AGATAGCCGA TTTCGGATTA 
TCTAATATGA TGTCAGATGG TGAATTTCTG AGAACTAGTT GCGGATCTCC AAATTATGCA 
GCACCTGAAG TCATCTCAGG CAGATTGTAT GCAGGTCCTG AAGTTGATAT CTGGAGCTGT 
GGTGTTATCT TGTATGCTCT TCTTTGTGGC ACCCTCCCAT TTGATGATGA GCATGTACCT 
ACGTTATTTA AGAAGATCCG AGGGGGTGTC TTTTATATCC CAGAATATCT CAATCGTTCT 
GTCGCCACTC TCCTGATGCA TATGCTGCAG GTTGACCCAC TGAAACGAGC AACTATCAAA 
GACATAAGAG AGCATGAATG GTTTAAACAA GATTTGCCCA GTTACTTATT TCCTGAAGAC 
CCTTCCTATG ATGCTAACGT CATTGATGAT GAGGCTGTGA AAGAAGTGTG TGAAAAATTT 
GAATGTACAG AATCAGAAGT AATGAACAGT TTATATAGTG GTGACCCTCA AGACCAGCTT 
GCAGTGGCTT ATCATCTTAT CATTGACAAT CGGAGAATAA TGAACCAAGC CAGTGAGTTC 
TACCTCGCCT CTAGTCCTCC ATCTGGTTCT TTTATGGATG ATAGTGCCAT GCATATTCCC 
CCAGGCCTGA AACCTCATCC AGAAAGGATG CCACCTCTTA TAGCAGACAG CCCCAAAGCA 
AGATGTCCAT TGGATGCACT GAATACGACT AAGCCCAAAT CTTTAGCTGT GAAAAAAGCC 
AAGTGGCATC TTGGAATCCG AAGTCAGAGC AAACCGTATG ACATTATGGC TGAAGTTTAC 
CGAGCTATGA AGCAGCTGGA TTTTGAATGG AAGGTAGTGA ATGCATACCA TCTTCGTGTA 
AGAAGAAAAA ATCCAGTGAC TGGCAATTAC GTGAAAATGA GCTTACAACT TTACCTGGTT 
GATAACAGGA GCTATCTTTT GGACTTTAAA AGCATTGATG ATGAAGTAGT GGAGCAGAGA 
TCTGGTTCCT CAACACCTCA GCGTTCCTGT TCTGCTGCTG GCTTACACAG ACCAAGATCA 
AGTTTTGATT CCACAACTGC AGAGAGCCAT TCACTTTCTG GCTCTCTCAC TGGCTCTTTG 
ACCGGAAGCA CATTGTCTTC AGTTTCACCT CGCCTGGGCA GTCACACCAT GGATTTTTTT 
GAAATGTGTG CCAGTCTGAT TACTACTTTA GCCCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006243.2
CDS	75..1733
Translation	MAEKQKHDGRVKIGHYVLGDTLGVGTFGKVKIGEHQLTGHKVAVKILNRQKIRSLDVVGKIKREIQNLKLFRHPHIIKLYQVISTPTDFFMVMEYVSGGELFDYICKHGRVEEMEARRLFQQILSAVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSCGVILYALLCGTLPFDDEHVPTLFKKIRGGVFYIPEYLNRSVATLLMHMLQVDPLKRATIKDIREHEWFKQDLPSYLFPEDPSYDANVIDDEAVKEVCEKFECTESEVMNSLYSGDPQDQLAVAYHLIIDNRRIMNQASEFYLASSPPSGSFMDDSAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKPKSLAVKKAKWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVVNAYHLRVRRKNPVTGNYVKMSLQLYLVDNRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRPRSSFDSTTAESHSLSGSLTGSLTGSTLSSVSPRLGSHTMDFFEMCASLITTLAR

Target ORF information:

RefSeq Version	NM_006252.4
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase AMP-activated catalytic subunit alpha 2 (PRKAA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006252.4

ORF Insert Sequence:

ATGGCTGAGA AGCAGAAGCA CGACGGGCGG GTGAAGATCG GACACTACGT GCTGGGCGAC 
ACGCTGGGCG TCGGCACCTT CGGCAAAGTG AAGATTGGAG AACATCAATT AACAGGCCAT 
AAAGTGGCAG TTAAAATCTT AAATAGACAG AAGATTCGCA GTTTAGATGT TGTTGGAAAA 
ATAAAACGAG AAATTCAAAA TCTAAAACTC TTTCGTCATC CTCATATTAT CAAACTATAC 
CAGGTGATCA GCACTCCAAC AGATTTTTTT ATGGTAATGG AATATGTGTC TGGAGGTGAA 
TTATTTGACT ACATCTGTAA GCATGGACGG GTTGAAGAGA TGGAAGCCAG GCGGCTCTTT 
CAGCAGATTC TGTCTGCTGT GGATTACTGT CATAGGCATA TGGTTGTTCA TCGAGACCTG 
AAACCAGAGA ATGTCCTGTT GGATGCACAC ATGAATGCCA AGATAGCCGA TTTCGGATTA 
TCTAATATGA TGTCAGATGG TGAATTTCTG AGAACTAGTT GCGGATCTCC AAATTATGCA 
GCACCTGAAG TCATCTCAGG CAGATTGTAT GCAGGTCCTG AAGTTGATAT CTGGAGCTGT 
GGTGTTATCT TGTATGCTCT TCTTTGTGGC ACCCTCCCAT TTGATGATGA GCATGTACCT 
ACGTTATTTA AGAAGATCCG AGGGGGTGTC TTTTATATCC CAGAATATCT CAATCGTTCT 
GTCGCCACTC TCCTGATGCA TATGCTGCAG GTTGACCCAC TGAAACGAGC AACTATCAAA 
GACATAAGAG AGCATGAATG GTTTAAACAA GATTTGCCCA GTTACTTATT TCCTGAAGAC 
CCTTCCTATG ATGCTAACGT CATTGATGAT GAGGCTGTGA AAGAAGTGTG TGAAAAATTT 
GAATGTACAG AATCAGAAGT AATGAACAGT TTATATAGTG GTGACCCTCA AGACCAGCTT 
GCAGTGGCTT ATCATCTTAT CATTGACAAT CGGAGAATAA TGAACCAAGC CAGTGAGTTC 
TACCTCGCCT CTAGTCCTCC ATCTGGTTCT TTTATGGATG ATAGTGCCAT GCATATTCCC 
CCAGGCCTGA AACCTCATCC AGAAAGGATG CCACCTCTTA TAGCAGACAG CCCCAAAGCA 
AGATGTCCAT TGGATGCACT GAATACGACT AAGCCCAAAT CTTTAGCTGT GAAAAAAGCC 
AAGTGGCATC TTGGAATCCG AAGTCAGAGC AAACCGTATG ACATTATGGC TGAAGTTTAC 
CGAGCTATGA AGCAGCTGGA TTTTGAATGG AAGGTAGTGA ATGCATACCA TCTTCGTGTA 
AGAAGAAAAA ATCCAGTGAC TGGCAATTAC GTGAAAATGA GCTTACAACT TTACCTGGTT 
GATAACAGGA GCTATCTTTT GGACTTTAAA AGCATTGATG ATGAAGTAGT GGAGCAGAGA 
TCTGGTTCCT CAACACCTCA GCGTTCCTGT TCTGCTGCTG GCTTACACAG ACCAAGATCA 
AGTTTTGATT CCACAACTGC AGAGAGCCAT TCACTTTCTG GCTCTCTCAC TGGCTCTTTG 
ACCGGAAGCA CATTGTCTTC AGTTTCACCT CGCCTGGGCA GTCACACCAT GGATTTTTTT 
GAAATGTGTG CCAGTCTGAT TACTACTTTA GCCCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKAA2 ( XM_017001693.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857182.1 Homo sapiens protein kinase AMP-activated catalytic subunit alpha 2 (PRKAA2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu90199
Clone ID Related Accession (Same CDS sequence)	XM_017001693.1 , XM_017001694.1 , XM_017001692.1
Accession Version	XM_017001693.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	5'-AMP-activated protein kinase catalytic subunit alpha-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAATGG AATATGTGTC TGGAGGTGAA TTATTTGACT ACATCTGTAA GCATGGACGG 
GTTGAAGAGA TGGAAGCCAG GCGGCTCTTT CAGCAGATTC TGTCTGCTGT GGATTACTGT 
CATAGGCATA TGGTTGTTCA TCGAGACCTG AAACCAGAGA ATGTCCTGTT GGATGCACAC 
ATGAATGCCA AGATAGCCGA TTTCGGATTA TCTAATATGA TGTCAGATGG TGAATTTCTG 
AGAACTAGTT GCGGATCTCC AAATTATGCA GCACCTGAAG TCATCTCAGG CAGATTGTAT 
GCAGGTCCTG AAGTTGATAT CTGGAGCTGT GGTGTTATCT TGTATGCTCT TCTTTGTGGC 
ACCCTCCCAT TTGATGATGA GCATGTACCT ACGTTATTTA AGAAGATCCG AGGGGGTGTC 
TTTTATATCC CAGAATATCT CAATCGTTCT GTCGCCACTC TCCTGATGCA TATGCTGCAG 
GTTGACCCAC TGAAACGAGC AACTATCAAA GACATAAGAG AGCATGAATG GTTTAAACAA 
GATTTGCCCA GTTACTTATT TCCTGAAGAC CCTTCCTATG ATGCTAACGT CATTGATGAT 
GAGGCTGTGA AAGAAGTGTG TGAAAAATTT GAATGTACAG AATCAGAAGT AATGAACAGT 
TTATATAGTG GTGACCCTCA AGACCAGCTT GCAGTGGCTT ATCATCTTAT CATTGACAAT 
CGGAGAATAA TGAACCAAGC CAGTGAGTTC TACCTCGCCT CTAGTCCTCC ATCTGGTTCT 
TTTATGGATG ATAGTGCCAT GCATATTCCC CCAGGCCTGA AACCTCATCC AGAAAGGATG 
CCACCTCTTA TAGCAGACAG CCCCAAAGCA AGATGTCCAT TGGATGCACT GAATACGACT 
AAGCCCAAAT CTTTAGCTGT GAAAAAAGCC AAGTGGCATC TTGGAATCCG AAGTCAGAGC 
AAACCGTATG ACATTATGGC TGAAGTTTAC CGAGCTATGA AGCAGCTGGA TTTTGAATGG 
AAGGTAGTGA ATGCATACCA TCTTCGTGTA AGAAGAAAAA ATCCAGTGAC TGGCAATTAC 
GTGAAAATGA GCTTACAACT TTACCTGGTT GATAACAGGA GCTATCTTTT GGACTTTAAA 
AGCATTGATG ATGAAGTAGT GGAGCAGAGA TCTGGTTCCT CAACACCTCA GCGTTCCTGT 
TCTGCTGCTG GCTTACACAG ACCAAGATCA AGTTTTGATT CCACAACTGC AGAGAGCCAT 
TCACTTTCTG GCTCTCTCAC TGGCTCTTTG ACCGGAAGCA CATTGTCTTC AGTTTCACCT 
CGCCTGGGCA GTCACACCAT GGATTTTTTT GAAATGTGTG CCAGTCTGAT TACTACTTTA 
GCCCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857182.1
CDS	427..1815
Translation	MVMEYVSGGELFDYICKHGRVEEMEARRLFQQILSAVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSCGVILYALLCGTLPFDDEHVPTLFKKIRGGVFYIPEYLNRSVATLLMHMLQVDPLKRATIKDIREHEWFKQDLPSYLFPEDPSYDANVIDDEAVKEVCEKFECTESEVMNSLYSGDPQDQLAVAYHLIIDNRRIMNQASEFYLASSPPSGSFMDDSAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKPKSLAVKKAKWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVVNAYHLRVRRKNPVTGNYVKMSLQLYLVDNRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRPRSSFDSTTAESHSLSGSLTGSLTGSTLSSVSPRLGSHTMDFFEMCASLITTLAR

Target ORF information:

RefSeq Version	XM_017001693.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase AMP-activated catalytic subunit alpha 2 (PRKAA2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001693.1

ORF Insert Sequence:

ATGGTAATGG AATATGTGTC TGGAGGTGAA TTATTTGACT ACATCTGTAA GCATGGACGG 
GTTGAAGAGA TGGAAGCCAG GCGGCTCTTT CAGCAGATTC TGTCTGCTGT GGATTACTGT 
CATAGGCATA TGGTTGTTCA TCGAGACCTG AAACCAGAGA ATGTCCTGTT GGATGCACAC 
ATGAATGCCA AGATAGCCGA TTTCGGATTA TCTAATATGA TGTCAGATGG TGAATTTCTG 
AGAACTAGTT GCGGATCTCC AAATTATGCA GCACCTGAAG TCATCTCAGG CAGATTGTAT 
GCAGGTCCTG AAGTTGATAT CTGGAGCTGT GGTGTTATCT TGTATGCTCT TCTTTGTGGC 
ACCCTCCCAT TTGATGATGA GCATGTACCT ACGTTATTTA AGAAGATCCG AGGGGGTGTC 
TTTTATATCC CAGAATATCT CAATCGTTCT GTCGCCACTC TCCTGATGCA TATGCTGCAG 
GTTGACCCAC TGAAACGAGC AACTATCAAA GACATAAGAG AGCATGAATG GTTTAAACAA 
GATTTGCCCA GTTACTTATT TCCTGAAGAC CCTTCCTATG ATGCTAACGT CATTGATGAT 
GAGGCTGTGA AAGAAGTGTG TGAAAAATTT GAATGTACAG AATCAGAAGT AATGAACAGT 
TTATATAGTG GTGACCCTCA AGACCAGCTT GCAGTGGCTT ATCATCTTAT CATTGACAAT 
CGGAGAATAA TGAACCAAGC CAGTGAGTTC TACCTCGCCT CTAGTCCTCC ATCTGGTTCT 
TTTATGGATG ATAGTGCCAT GCATATTCCC CCAGGCCTGA AACCTCATCC AGAAAGGATG 
CCACCTCTTA TAGCAGACAG CCCCAAAGCA AGATGTCCAT TGGATGCACT GAATACGACT 
AAGCCCAAAT CTTTAGCTGT GAAAAAAGCC AAGTGGCATC TTGGAATCCG AAGTCAGAGC 
AAACCGTATG ACATTATGGC TGAAGTTTAC CGAGCTATGA AGCAGCTGGA TTTTGAATGG 
AAGGTAGTGA ATGCATACCA TCTTCGTGTA AGAAGAAAAA ATCCAGTGAC TGGCAATTAC 
GTGAAAATGA GCTTACAACT TTACCTGGTT GATAACAGGA GCTATCTTTT GGACTTTAAA 
AGCATTGATG ATGAAGTAGT GGAGCAGAGA TCTGGTTCCT CAACACCTCA GCGTTCCTGT 
TCTGCTGCTG GCTTACACAG ACCAAGATCA AGTTTTGATT CCACAACTGC AGAGAGCCAT 
TCACTTTCTG GCTCTCTCAC TGGCTCTTTG ACCGGAAGCA CATTGTCTTC AGTTTCACCT 
CGCCTGGGCA GTCACACCAT GGATTTTTTT GAAATGTGTG CCAGTCTGAT TACTACTTTA 
GCCCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKAA2 ( XM_017001694.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857183.1 Homo sapiens protein kinase AMP-activated catalytic subunit alpha 2 (PRKAA2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu90199
Clone ID Related Accession (Same CDS sequence)	XM_017001693.1 , XM_017001694.1 , XM_017001692.1
Accession Version	XM_017001694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	5'-AMP-activated protein kinase catalytic subunit alpha-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAATGG AATATGTGTC TGGAGGTGAA TTATTTGACT ACATCTGTAA GCATGGACGG 
GTTGAAGAGA TGGAAGCCAG GCGGCTCTTT CAGCAGATTC TGTCTGCTGT GGATTACTGT 
CATAGGCATA TGGTTGTTCA TCGAGACCTG AAACCAGAGA ATGTCCTGTT GGATGCACAC 
ATGAATGCCA AGATAGCCGA TTTCGGATTA TCTAATATGA TGTCAGATGG TGAATTTCTG 
AGAACTAGTT GCGGATCTCC AAATTATGCA GCACCTGAAG TCATCTCAGG CAGATTGTAT 
GCAGGTCCTG AAGTTGATAT CTGGAGCTGT GGTGTTATCT TGTATGCTCT TCTTTGTGGC 
ACCCTCCCAT TTGATGATGA GCATGTACCT ACGTTATTTA AGAAGATCCG AGGGGGTGTC 
TTTTATATCC CAGAATATCT CAATCGTTCT GTCGCCACTC TCCTGATGCA TATGCTGCAG 
GTTGACCCAC TGAAACGAGC AACTATCAAA GACATAAGAG AGCATGAATG GTTTAAACAA 
GATTTGCCCA GTTACTTATT TCCTGAAGAC CCTTCCTATG ATGCTAACGT CATTGATGAT 
GAGGCTGTGA AAGAAGTGTG TGAAAAATTT GAATGTACAG AATCAGAAGT AATGAACAGT 
TTATATAGTG GTGACCCTCA AGACCAGCTT GCAGTGGCTT ATCATCTTAT CATTGACAAT 
CGGAGAATAA TGAACCAAGC CAGTGAGTTC TACCTCGCCT CTAGTCCTCC ATCTGGTTCT 
TTTATGGATG ATAGTGCCAT GCATATTCCC CCAGGCCTGA AACCTCATCC AGAAAGGATG 
CCACCTCTTA TAGCAGACAG CCCCAAAGCA AGATGTCCAT TGGATGCACT GAATACGACT 
AAGCCCAAAT CTTTAGCTGT GAAAAAAGCC AAGTGGCATC TTGGAATCCG AAGTCAGAGC 
AAACCGTATG ACATTATGGC TGAAGTTTAC CGAGCTATGA AGCAGCTGGA TTTTGAATGG 
AAGGTAGTGA ATGCATACCA TCTTCGTGTA AGAAGAAAAA ATCCAGTGAC TGGCAATTAC 
GTGAAAATGA GCTTACAACT TTACCTGGTT GATAACAGGA GCTATCTTTT GGACTTTAAA 
AGCATTGATG ATGAAGTAGT GGAGCAGAGA TCTGGTTCCT CAACACCTCA GCGTTCCTGT 
TCTGCTGCTG GCTTACACAG ACCAAGATCA AGTTTTGATT CCACAACTGC AGAGAGCCAT 
TCACTTTCTG GCTCTCTCAC TGGCTCTTTG ACCGGAAGCA CATTGTCTTC AGTTTCACCT 
CGCCTGGGCA GTCACACCAT GGATTTTTTT GAAATGTGTG CCAGTCTGAT TACTACTTTA 
GCCCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857183.1
CDS	335..1723
Translation	MVMEYVSGGELFDYICKHGRVEEMEARRLFQQILSAVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSCGVILYALLCGTLPFDDEHVPTLFKKIRGGVFYIPEYLNRSVATLLMHMLQVDPLKRATIKDIREHEWFKQDLPSYLFPEDPSYDANVIDDEAVKEVCEKFECTESEVMNSLYSGDPQDQLAVAYHLIIDNRRIMNQASEFYLASSPPSGSFMDDSAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKPKSLAVKKAKWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVVNAYHLRVRRKNPVTGNYVKMSLQLYLVDNRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRPRSSFDSTTAESHSLSGSLTGSLTGSTLSSVSPRLGSHTMDFFEMCASLITTLAR

Target ORF information:

RefSeq Version	XM_017001694.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase AMP-activated catalytic subunit alpha 2 (PRKAA2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001694.1

ORF Insert Sequence:

ATGGTAATGG AATATGTGTC TGGAGGTGAA TTATTTGACT ACATCTGTAA GCATGGACGG 
GTTGAAGAGA TGGAAGCCAG GCGGCTCTTT CAGCAGATTC TGTCTGCTGT GGATTACTGT 
CATAGGCATA TGGTTGTTCA TCGAGACCTG AAACCAGAGA ATGTCCTGTT GGATGCACAC 
ATGAATGCCA AGATAGCCGA TTTCGGATTA TCTAATATGA TGTCAGATGG TGAATTTCTG 
AGAACTAGTT GCGGATCTCC AAATTATGCA GCACCTGAAG TCATCTCAGG CAGATTGTAT 
GCAGGTCCTG AAGTTGATAT CTGGAGCTGT GGTGTTATCT TGTATGCTCT TCTTTGTGGC 
ACCCTCCCAT TTGATGATGA GCATGTACCT ACGTTATTTA AGAAGATCCG AGGGGGTGTC 
TTTTATATCC CAGAATATCT CAATCGTTCT GTCGCCACTC TCCTGATGCA TATGCTGCAG 
GTTGACCCAC TGAAACGAGC AACTATCAAA GACATAAGAG AGCATGAATG GTTTAAACAA 
GATTTGCCCA GTTACTTATT TCCTGAAGAC CCTTCCTATG ATGCTAACGT CATTGATGAT 
GAGGCTGTGA AAGAAGTGTG TGAAAAATTT GAATGTACAG AATCAGAAGT AATGAACAGT 
TTATATAGTG GTGACCCTCA AGACCAGCTT GCAGTGGCTT ATCATCTTAT CATTGACAAT 
CGGAGAATAA TGAACCAAGC CAGTGAGTTC TACCTCGCCT CTAGTCCTCC ATCTGGTTCT 
TTTATGGATG ATAGTGCCAT GCATATTCCC CCAGGCCTGA AACCTCATCC AGAAAGGATG 
CCACCTCTTA TAGCAGACAG CCCCAAAGCA AGATGTCCAT TGGATGCACT GAATACGACT 
AAGCCCAAAT CTTTAGCTGT GAAAAAAGCC AAGTGGCATC TTGGAATCCG AAGTCAGAGC 
AAACCGTATG ACATTATGGC TGAAGTTTAC CGAGCTATGA AGCAGCTGGA TTTTGAATGG 
AAGGTAGTGA ATGCATACCA TCTTCGTGTA AGAAGAAAAA ATCCAGTGAC TGGCAATTAC 
GTGAAAATGA GCTTACAACT TTACCTGGTT GATAACAGGA GCTATCTTTT GGACTTTAAA 
AGCATTGATG ATGAAGTAGT GGAGCAGAGA TCTGGTTCCT CAACACCTCA GCGTTCCTGT 
TCTGCTGCTG GCTTACACAG ACCAAGATCA AGTTTTGATT CCACAACTGC AGAGAGCCAT 
TCACTTTCTG GCTCTCTCAC TGGCTCTTTG ACCGGAAGCA CATTGTCTTC AGTTTCACCT 
CGCCTGGGCA GTCACACCAT GGATTTTTTT GAAATGTGTG CCAGTCTGAT TACTACTTTA 
GCCCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKAA2 ( XM_017001692.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857181.1 Homo sapiens protein kinase AMP-activated catalytic subunit alpha 2 (PRKAA2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu90199
Clone ID Related Accession (Same CDS sequence)	XM_017001693.1 , XM_017001694.1 , XM_017001692.1
Accession Version	XM_017001692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	5'-AMP-activated protein kinase catalytic subunit alpha-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAATGG AATATGTGTC TGGAGGTGAA TTATTTGACT ACATCTGTAA GCATGGACGG 
GTTGAAGAGA TGGAAGCCAG GCGGCTCTTT CAGCAGATTC TGTCTGCTGT GGATTACTGT 
CATAGGCATA TGGTTGTTCA TCGAGACCTG AAACCAGAGA ATGTCCTGTT GGATGCACAC 
ATGAATGCCA AGATAGCCGA TTTCGGATTA TCTAATATGA TGTCAGATGG TGAATTTCTG 
AGAACTAGTT GCGGATCTCC AAATTATGCA GCACCTGAAG TCATCTCAGG CAGATTGTAT 
GCAGGTCCTG AAGTTGATAT CTGGAGCTGT GGTGTTATCT TGTATGCTCT TCTTTGTGGC 
ACCCTCCCAT TTGATGATGA GCATGTACCT ACGTTATTTA AGAAGATCCG AGGGGGTGTC 
TTTTATATCC CAGAATATCT CAATCGTTCT GTCGCCACTC TCCTGATGCA TATGCTGCAG 
GTTGACCCAC TGAAACGAGC AACTATCAAA GACATAAGAG AGCATGAATG GTTTAAACAA 
GATTTGCCCA GTTACTTATT TCCTGAAGAC CCTTCCTATG ATGCTAACGT CATTGATGAT 
GAGGCTGTGA AAGAAGTGTG TGAAAAATTT GAATGTACAG AATCAGAAGT AATGAACAGT 
TTATATAGTG GTGACCCTCA AGACCAGCTT GCAGTGGCTT ATCATCTTAT CATTGACAAT 
CGGAGAATAA TGAACCAAGC CAGTGAGTTC TACCTCGCCT CTAGTCCTCC ATCTGGTTCT 
TTTATGGATG ATAGTGCCAT GCATATTCCC CCAGGCCTGA AACCTCATCC AGAAAGGATG 
CCACCTCTTA TAGCAGACAG CCCCAAAGCA AGATGTCCAT TGGATGCACT GAATACGACT 
AAGCCCAAAT CTTTAGCTGT GAAAAAAGCC AAGTGGCATC TTGGAATCCG AAGTCAGAGC 
AAACCGTATG ACATTATGGC TGAAGTTTAC CGAGCTATGA AGCAGCTGGA TTTTGAATGG 
AAGGTAGTGA ATGCATACCA TCTTCGTGTA AGAAGAAAAA ATCCAGTGAC TGGCAATTAC 
GTGAAAATGA GCTTACAACT TTACCTGGTT GATAACAGGA GCTATCTTTT GGACTTTAAA 
AGCATTGATG ATGAAGTAGT GGAGCAGAGA TCTGGTTCCT CAACACCTCA GCGTTCCTGT 
TCTGCTGCTG GCTTACACAG ACCAAGATCA AGTTTTGATT CCACAACTGC AGAGAGCCAT 
TCACTTTCTG GCTCTCTCAC TGGCTCTTTG ACCGGAAGCA CATTGTCTTC AGTTTCACCT 
CGCCTGGGCA GTCACACCAT GGATTTTTTT GAAATGTGTG CCAGTCTGAT TACTACTTTA 
GCCCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857181.1
CDS	1807..3195
Translation	MVMEYVSGGELFDYICKHGRVEEMEARRLFQQILSAVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSCGVILYALLCGTLPFDDEHVPTLFKKIRGGVFYIPEYLNRSVATLLMHMLQVDPLKRATIKDIREHEWFKQDLPSYLFPEDPSYDANVIDDEAVKEVCEKFECTESEVMNSLYSGDPQDQLAVAYHLIIDNRRIMNQASEFYLASSPPSGSFMDDSAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKPKSLAVKKAKWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVVNAYHLRVRRKNPVTGNYVKMSLQLYLVDNRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRPRSSFDSTTAESHSLSGSLTGSLTGSTLSSVSPRLGSHTMDFFEMCASLITTLAR

Target ORF information:

RefSeq Version	XM_017001692.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase AMP-activated catalytic subunit alpha 2 (PRKAA2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001692.1

ORF Insert Sequence:

ATGGTAATGG AATATGTGTC TGGAGGTGAA TTATTTGACT ACATCTGTAA GCATGGACGG 
GTTGAAGAGA TGGAAGCCAG GCGGCTCTTT CAGCAGATTC TGTCTGCTGT GGATTACTGT 
CATAGGCATA TGGTTGTTCA TCGAGACCTG AAACCAGAGA ATGTCCTGTT GGATGCACAC 
ATGAATGCCA AGATAGCCGA TTTCGGATTA TCTAATATGA TGTCAGATGG TGAATTTCTG 
AGAACTAGTT GCGGATCTCC AAATTATGCA GCACCTGAAG TCATCTCAGG CAGATTGTAT 
GCAGGTCCTG AAGTTGATAT CTGGAGCTGT GGTGTTATCT TGTATGCTCT TCTTTGTGGC 
ACCCTCCCAT TTGATGATGA GCATGTACCT ACGTTATTTA AGAAGATCCG AGGGGGTGTC 
TTTTATATCC CAGAATATCT CAATCGTTCT GTCGCCACTC TCCTGATGCA TATGCTGCAG 
GTTGACCCAC TGAAACGAGC AACTATCAAA GACATAAGAG AGCATGAATG GTTTAAACAA 
GATTTGCCCA GTTACTTATT TCCTGAAGAC CCTTCCTATG ATGCTAACGT CATTGATGAT 
GAGGCTGTGA AAGAAGTGTG TGAAAAATTT GAATGTACAG AATCAGAAGT AATGAACAGT 
TTATATAGTG GTGACCCTCA AGACCAGCTT GCAGTGGCTT ATCATCTTAT CATTGACAAT 
CGGAGAATAA TGAACCAAGC CAGTGAGTTC TACCTCGCCT CTAGTCCTCC ATCTGGTTCT 
TTTATGGATG ATAGTGCCAT GCATATTCCC CCAGGCCTGA AACCTCATCC AGAAAGGATG 
CCACCTCTTA TAGCAGACAG CCCCAAAGCA AGATGTCCAT TGGATGCACT GAATACGACT 
AAGCCCAAAT CTTTAGCTGT GAAAAAAGCC AAGTGGCATC TTGGAATCCG AAGTCAGAGC 
AAACCGTATG ACATTATGGC TGAAGTTTAC CGAGCTATGA AGCAGCTGGA TTTTGAATGG 
AAGGTAGTGA ATGCATACCA TCTTCGTGTA AGAAGAAAAA ATCCAGTGAC TGGCAATTAC 
GTGAAAATGA GCTTACAACT TTACCTGGTT GATAACAGGA GCTATCTTTT GGACTTTAAA 
AGCATTGATG ATGAAGTAGT GGAGCAGAGA TCTGGTTCCT CAACACCTCA GCGTTCCTGT 
TCTGCTGCTG GCTTACACAG ACCAAGATCA AGTTTTGATT CCACAACTGC AGAGAGCCAT 
TCACTTTCTG GCTCTCTCAC TGGCTCTTTG ACCGGAAGCA CATTGTCTTC AGTTTCACCT 
CGCCTGGGCA GTCACACCAT GGATTTTTTT GAAATGTGTG CCAGTCTGAT TACTACTTTA 
GCCCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

AMPK-AKT double negative feedback loop in breast cancer cells regulates their adaptation to matrix deprivation.
Cancer research(2018 Jan)
Saha M,Kumar S,Bukhari S,Balaji SA,Kumar P,Hindupur SK,Rangarajan A

High-intensity high-volume swimming induces more robust signaling through PGC-1? and AMPK activation than sprint interval swimming in m. triceps brachii.
PloS one12(10)e0185494(2017)
Casuso RA,Plaza-D?az J,Ruiz-Ojeda FJ,Arag?n-Vela J,Robles-Sanchez C,Nordsborg NB,Hebberecht M,Salmeron LM,Huertas JR

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PRKAA2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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