ASIC4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ASIC4
Entrez Gene ID	55515
Full Name	acid sensing ion channel subunit family member 4
Synonyms	ACCN4,BNAC4
General protein information	Preferred Names acid sensing ion channel subunit family member 4 Names acid-sensing ion channel 4 acid sensing ion channel family member 4 acid-sensing (proton-gated) ion channel family member 4 amiloride-sensitive cation channel 4, pituitary amiloride-sensitive cation channel family member 4, pituitary brain sodium channel 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q35
Summary	This gene belongs to the superfamily of acid-sensing ion channels, which are proton-gated, amiloride-sensitive sodium channels. These channels have been implicated in synaptic transmission, pain perception as well as mechanoperception. This gene is predominantly expressed in the pituitary gland, and was considered a candidate for paroxysmal dystonic choreoathetosis (PDC), a movement disorder, however, no correlation was found between mutations in this gene and PDC. [provided by RefSeq, Feb 2012].
Disorder MIM:	606715

mRNA and Protein(s)

mRNA	Protein	Name
NM_018674.5	NP_061144.3	acid-sensing ion channel 4 isoform 1
NM_182847.2	NP_878267.2	acid-sensing ion channel 4 isoform 2
XM_017004439.1	XP_016859928.1	acid-sensing ion channel 4 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ASIC4	NP_878267.2
	LOC703972	XP_002808071.1
Rattus norvegicus (Norway rat)	Asic4	NP_071570.2
Pan troglodytes (chimpanzee)	ACCN4	XP_001163732.2
Mus musculus (house mouse)	Asic4	NP_898843.1
Danio rerio (zebrafish)	asic4b	NP_999951.1
Bos taurus (cattle)	ASIC4	XP_003585817.2
Xenopus tropicalis (tropical clawed frog)	asic4	XP_002934008.2
Canis lupus familiaris (dog)	ASIC4	XP_005640766.1
Gallus gallus (chicken)	ASIC4	XP_004942894.1
Danio rerio (zebrafish)	asic4a	NP_999952.1

Gene Ontology
Bibliography

Related articles in PubMed

Acid-sensing ion channel (ASIC) 4 predominantly localizes to an early endosome-related organelle upon heterologous expression.
Schwartz V, Friedrich K, Polleichtner G, Gr?nder S
Scientific reports518242(2015 Dec)

Regulation of ASIC activity by ASIC4--new insights into ASIC channel function revealed by a yeast two-hybrid assay.
Donier E, Rugiero F, Jacob C, Wood JN
The European journal of neuroscience28(1)74-86(2008 Jul)

Acid-sensing ion channel (ASIC) 4 gene: physical mapping, genomic organisation, and evaluation as a candidate for paroxysmal dystonia.
Gr?nder S, Geisler HS, Rainier S, Fink JK
European journal of human genetics : EJHG9(9)672-6(2001 Sep)

A new member of acid-sensing ion channels from pituitary gland.
Gr?nder S, Geissler HS, B?ssler EL, Ruppersberg JP
Neuroreport11(8)1607-11(2000 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Acid-sensing ion channel (ASIC) 4 predominantly localizes to an early endosome-related organelle upon heterologous expression
Title: Acid-sensing ion channel (ASIC) 4 predominantly localizes to an early endosome-related organelle upon heterologous expression.

Data show that transfected ASIC4 is targeted to the plasma membrane in CHO-K1 cells, where it associates with ASIC1a and downregulates exogenous ASIC1a expression.
Title: Regulation of ASIC activity by ASIC4--new insights into ASIC channel function revealed by a yeast two-hybrid assay.

The following ASIC4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASIC4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10878	NM_182847.2 Latest version!	Homo sapiens acid sensing ion channel subunit family member 4 (ASIC4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu89634	XM_017004439.1 Latest version!	Homo sapiens acid sensing ion channel subunit family member 4 (ASIC4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu69428	NM_018674.5 Latest version!	Homo sapiens acid sensing ion channel subunit family member 4 (ASIC4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ASIC4 ( NM_182847.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_878267.2 Homo sapiens acid sensing ion channel subunit family member 4 (ASIC4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10878
Clone ID Related Accession (Same CDS sequence)	NM_182847.2
Accession Version	NM_182847.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Homo sapiens(human)
Product	acid-sensing ion channel 4 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC031812.1, BC010439.1 and AC009955.4. On Aug 28, 2009 this sequence version replaced NM_182847.1. Transcript Variant: This variant (2) lacks an exon in the central coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC010439.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAGCG GAGCGGCTGG GGCTGCGCGG CGTGGCGGAG CAGCGCTCGC TCCCTCGCTC 
ACTCGCTCGC TCGCAGGGAC ACACGCAGGG GCTGACAGCT GTGCTGGTGC TGATAAGGGA 
AGCCACAAGG AGACGATCGA GGAGAGAGAC AAGCGGCAGC AGAGGCAGCA GCGGCAGAGG 
CAGCACCAGG GCTGCGGAGC TGCTGGGAGT GGGAGTGACT CCCCCACCTC GGGCCCCCAC 
CCTGTCCCTG TCCTCTTCCC GCTTGCCCTG AGTTTAGAAG AGCAGCCGCT GCCACCACTG 
CCACTCGGGA GGGCACCAGG GCTGCTGGCT AGGGAGGGAC AGGGCAGGGA GGCTCTGGCC 
AGTCCCAGCA GCCGGGGACA GATGCCGATC GAGATTGTGT GCAAAATCAA ATTTGCTGAG 
GAGGATGCGA AACCCAAGGA GAAGGAGGCA GGGGATGAGC AGAGCCTCCT CGGGGCTGTT 
GCCCCTGGAG CAGCCCCCCG AGACCTGGCC ACCTTTGCCA GCACCAGCAC CCTGCATGGA 
CTGGGCCGGG CCTGTGGCCC AGGCCCCCAC GGACTGCGCA GAACCCTGTG GGCACTGGCC 
CTACTCACCT CGCTGGCTGC CTTCCTGTAC CAGGCGGCTG GCCTGGCCCG GGGCTACCTG 
ACCCGGCCTC ACCTGGTGGC AATGGACCCC GCTGCCCCAG CCCCAGTGGC GGGCTTCCCG 
GCTGTCACCC TCTGCAATAT CAACCGCTTC CGGCATTCGG CACTCAGCGA TGCCGACATC 
TTCCACCTGG CCAATCTGAC AGGGCTGCCC CCCAAAGACC GGGATGGGCA CCGTGCGGCT 
GGCCTGCGCT ACCCAGAGCC TGACATGGTA GACATCCTCA ACCGCACTGG CCACCAGCTC 
GCCGACATGC TTAAGAGCTG CAACTTCAGT GGGCATCACT GCTCCGCCAG CAACTTCTCT 
GTGGTCTATA CTCGCTATGG GAAGTGTTAC ACCTTCAACG CGGACCCGCG GAGCTCGCTG 
CCCAGCCGGG CAGGGGGCAT GGGCAGTGGC CTGGAGATCA TGCTGGACAT CCAGCAGGAG 
GAGTACCTGC CCATCTGGAG GGAGACAAAT GAGACGTCGT TTGAGGCAGG TATTCGGGTG 
CAGATCCACA GCCAGGAGGA GCCGCCCTAC ATCCACCAGC TGGGGTTCGG GGTGTCCCCA 
GGCTTCCAGA CCTTTGTGTC CTGCCAGGAA CAGCGGCTGA CCTACCTGCC CCAGCCCTGG 
GGCAACTGCC GCGCAGAGAG TGAGCTCAGG GAGCCTGAGC TTCAGGGCTA CTCGGCCTAC 
AGTGTGTCTG CCTGCCGGCT GCGCTGTGAA AAGGAGGCCG TGCTTCAGCG CTGCCACTGC 
CGGATGGTGC ACATGCCAGA CTCCCTGGGT GGGGGCCCTG AGGGCCCGTG CTTCTGCCCC 
ACCCCCTGCA ACCTGACACG CTATGGGAAA GAGATCTCCA TGGTCAGGAT CCCCAACAGG 
GGCTCAGCCC GGTACCTGGC GAGGAAGTAC AACCGCAACG AGACCTACAT ACGGGAGAAC 
TTCCTGGTCC TAGATGTCTT CTTTGAGGCC CTGACCTCTG AAGCCATGGA GCAGCGAGCA 
GCCTATGGCC TGTCAGCCCT GCTGGGAGAC CTCGGGGGAC AGATGGGCCT GTTCATTGGG 
GCCAGCATCC TCACGTTGCT GGAGATCCTC GACTACATCT ATGAGGTGTC CTGGGATCGA 
CTGAAGCGGG TATGGAGGCG TCCCAAGACC CCCCTGCGGA CCTCCACTGG GGGCATCTCC 
ACTTTGGGGC TTCAGGAGCT GAAGGAACAG AGTCCCTGCC CGAGCCGGGG CCGAGTGGAG 
GGTGGGGGGG TCAGCAGTCT GCTCCCCAAT CACCACCACC CCCACGGTCC CCCAGGAGGT 
CTCTTTGAAG ATTTTGCTTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_878267.2
CDS	175..2118
Translation	MLSGAAGAARRGGAALAPSLTRSLAGTHAGADSCAGADKGSHKETIEERDKRQQRQQRQRQHQGCGAAGSGSDSPTSGPHPVPVLFPLALSLEEQPLPPLPLGRAPGLLAREGQGREALASPSSRGQMPIEIVCKIKFAEEDAKPKEKEAGDEQSLLGAVAPGAAPRDLATFASTSTLHGLGRACGPGPHGLRRTLWALALLTSLAAFLYQAAGLARGYLTRPHLVAMDPAAPAPVAGFPAVTLCNINRFRHSALSDADIFHLANLTGLPPKDRDGHRAAGLRYPEPDMVDILNRTGHQLADMLKSCNFSGHHCSASNFSVVYTRYGKCYTFNADPRSSLPSRAGGMGSGLEIMLDIQQEEYLPIWRETNETSFEAGIRVQIHSQEEPPYIHQLGFGVSPGFQTFVSCQEQRLTYLPQPWGNCRAESELREPELQGYSAYSVSACRLRCEKEAVLQRCHCRMVHMPDSLGGGPEGPCFCPTPCNLTRYGKEISMVRIPNRGSARYLARKYNRNETYIRENFLVLDVFFEALTSEAMEQRAAYGLSALLGDLGGQMGLFIGASILTLLEILDYIYEVSWDRLKRVWRRPKTPLRTSTGGISTLGLQELKEQSPCPSRGRVEGGGVSSLLPNHHHPHGPPGGLFEDFAC

Target ORF information:

RefSeq Version	NM_182847.2
Organism	Homo sapiens(human)
Definition	Homo sapiens acid sensing ion channel subunit family member 4 (ASIC4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182847.2

ORF Insert Sequence:

ATGCTGAGCG GAGCGGCTGG GGCTGCGCGG CGTGGCGGAG CAGCGCTCGC TCCCTCGCTC 
ACTCGCTCGC TCGCAGGGAC ACACGCAGGG GCTGACAGCT GTGCTGGTGC TGATAAGGGA 
AGCCACAAGG AGACGATCGA GGAGAGAGAC AAGCGGCAGC AGAGGCAGCA GCGGCAGAGG 
CAGCACCAGG GCTGCGGAGC TGCTGGGAGT GGGAGTGACT CCCCCACCTC GGGCCCCCAC 
CCTGTCCCTG TCCTCTTCCC GCTTGCCCTG AGTTTAGAAG AGCAGCCGCT GCCACCACTG 
CCACTCGGGA GGGCACCAGG GCTGCTGGCT AGGGAGGGAC AGGGCAGGGA GGCTCTGGCC 
AGTCCCAGCA GCCGGGGACA GATGCCGATC GAGATTGTGT GCAAAATCAA ATTTGCTGAG 
GAGGATGCGA AACCCAAGGA GAAGGAGGCA GGGGATGAGC AGAGCCTCCT CGGGGCTGTT 
GCCCCTGGAG CAGCCCCCCG AGACCTGGCC ACCTTTGCCA GCACCAGCAC CCTGCATGGA 
CTGGGCCGGG CCTGTGGCCC AGGCCCCCAC GGACTGCGCA GAACCCTGTG GGCACTGGCC 
CTACTCACCT CGCTGGCTGC CTTCCTGTAC CAGGCGGCTG GCCTGGCCCG GGGCTACCTG 
ACCCGGCCTC ACCTGGTGGC AATGGACCCC GCTGCCCCAG CCCCAGTGGC GGGCTTCCCG 
GCTGTCACCC TCTGCAATAT CAACCGCTTC CGGCATTCGG CACTCAGCGA TGCCGACATC 
TTCCACCTGG CCAATCTGAC AGGGCTGCCC CCCAAAGACC GGGATGGGCA CCGTGCGGCT 
GGCCTGCGCT ACCCAGAGCC TGACATGGTA GACATCCTCA ACCGCACTGG CCACCAGCTC 
GCCGACATGC TTAAGAGCTG CAACTTCAGT GGGCATCACT GCTCCGCCAG CAACTTCTCT 
GTGGTCTATA CTCGCTATGG GAAGTGTTAC ACCTTCAACG CGGACCCGCG GAGCTCGCTG 
CCCAGCCGGG CAGGGGGCAT GGGCAGTGGC CTGGAGATCA TGCTGGACAT CCAGCAGGAG 
GAGTACCTGC CCATCTGGAG GGAGACAAAT GAGACGTCGT TTGAGGCAGG TATTCGGGTG 
CAGATCCACA GCCAGGAGGA GCCGCCCTAC ATCCACCAGC TGGGGTTCGG GGTGTCCCCA 
GGCTTCCAGA CCTTTGTGTC CTGCCAGGAA CAGCGGCTGA CCTACCTGCC CCAGCCCTGG 
GGCAACTGCC GCGCAGAGAG TGAGCTCAGG GAGCCTGAGC TTCAGGGCTA CTCGGCCTAC 
AGTGTGTCTG CCTGCCGGCT GCGCTGTGAA AAGGAGGCCG TGCTTCAGCG CTGCCACTGC 
CGGATGGTGC ACATGCCAGA CTCCCTGGGT GGGGGCCCTG AGGGCCCGTG CTTCTGCCCC 
ACCCCCTGCA ACCTGACACG CTATGGGAAA GAGATCTCCA TGGTCAGGAT CCCCAACAGG 
GGCTCAGCCC GGTACCTGGC GAGGAAGTAC AACCGCAACG AGACCTACAT ACGGGAGAAC 
TTCCTGGTCC TAGATGTCTT CTTTGAGGCC CTGACCTCTG AAGCCATGGA GCAGCGAGCA 
GCCTATGGCC TGTCAGCCCT GCTGGGAGAC CTCGGGGGAC AGATGGGCCT GTTCATTGGG 
GCCAGCATCC TCACGTTGCT GGAGATCCTC GACTACATCT ATGAGGTGTC CTGGGATCGA 
CTGAAGCGGG TATGGAGGCG TCCCAAGACC CCCCTGCGGA CCTCCACTGG GGGCATCTCC 
ACTTTGGGGC TTCAGGAGCT GAAGGAACAG AGTCCCTGCC CGAGCCGGGG CCGAGTGGAG 
GGTGGGGGGG TCAGCAGTCT GCTCCCCAAT CACCACCACC CCCACGGTCC CCCAGGAGGT 
CTCTTTGAAG ATTTTGCTTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASIC4 ( XM_017004439.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859928.1 Homo sapiens acid sensing ion channel subunit family member 4 (ASIC4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu89634
Clone ID Related Accession (Same CDS sequence)	XM_017004439.1
Accession Version	XM_017004439.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	acid-sensing ion channel 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGTG GCCTGGAGAT CATGCTGGAC ATCCAGCAGG AGGAGTACCT GCCCATCTGG 
AGGGAGACAA ATGAGACGTC GTTTGAGGCA GGTATTCGGG TGCAGATCCA CAGCCAGGAG 
GAGCCGCCCT ACATCCACCA GCTGGGGTTC GGGGTGTCCC CAGGCTTCCA GACCTTTGTG 
TCCTGCCAGG AACAGCGGCT GACCTACCTG CCCCAGCCCT GGGGCAACTG CCGCGCAGAG 
AGTGAGCTCA GGGAGCCTGA GCTTCAGGGC TACTCGGCCT ACAGTGTGTC TGCCTGCCGG 
CTGCGCTGTG AAAAGGAGGC CGTGCTTCAG CGCTGCCACT GCCGGATGGT GCACATGCCA 
GGCAATGAGA CCATCTGCCC ACCAAATATC TACATCGAGT GTGCAGACCA CACACTGGAC 
TCCCTGGGTG GGGGCCCTGA GGGCCCGTGC TTCTGCCCCA CCCCCTGCAA CCTGACACGC 
TATGGGAAAG AGATCTCCAT GGTCAGGATC CCCAACAGGG GCTCAGCCCG GTACCTGGCG 
AGGAAGTACA ACCGCAACGA GACCTACATA CGGGAGAACT TCCTGGTCCT AGATGTCTTC 
TTTGAGGCCC TGACCTCTGA AGCCATGGAG CAGCGAGCAG CCTATGGCCT GTCAGCCCTG 
CTGGGAGACC TCGGGGGACA GATGGGCCTG TTCATTGGGG CCAGCATCCT CACGTTGCTG 
GAGATCCTCG ACTACATCTA TGAGGTGTCC TGGGATCGAC TGAAGCGGGT ATGGAGGCGT 
CCCAAGACCC CCCTGCGGAC CTCCACTGGG GGCATCTCCA CTTTGGGGCT TCAGGAGCTG 
AAGGAACAGA GTCCCTGCCC GAGCCGGGGC CGAGTGGAGG GTGGGGGGGT CAGCAGTCTG 
CTCCCCAATC ACCACCACCC CCACGGTCCC CCAGGAGGTC TCTTTGAAGA TTTTGCTTGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859928.1
CDS	229..1191
Translation	MGSGLEIMLDIQQEEYLPIWRETNETSFEAGIRVQIHSQEEPPYIHQLGFGVSPGFQTFVSCQEQRLTYLPQPWGNCRAESELREPELQGYSAYSVSACRLRCEKEAVLQRCHCRMVHMPGNETICPPNIYIECADHTLDSLGGGPEGPCFCPTPCNLTRYGKEISMVRIPNRGSARYLARKYNRNETYIRENFLVLDVFFEALTSEAMEQRAAYGLSALLGDLGGQMGLFIGASILTLLEILDYIYEVSWDRLKRVWRRPKTPLRTSTGGISTLGLQELKEQSPCPSRGRVEGGGVSSLLPNHHHPHGPPGGLFEDFAC

Target ORF information:

RefSeq Version	XM_017004439.1
Organism	Homo sapiens(human)
Definition	Homo sapiens acid sensing ion channel subunit family member 4 (ASIC4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004439.1

ORF Insert Sequence:

ATGGGCAGTG GCCTGGAGAT CATGCTGGAC ATCCAGCAGG AGGAGTACCT GCCCATCTGG 
AGGGAGACAA ATGAGACGTC GTTTGAGGCA GGTATTCGGG TGCAGATCCA CAGCCAGGAG 
GAGCCGCCCT ACATCCACCA GCTGGGGTTC GGGGTGTCCC CAGGCTTCCA GACCTTTGTG 
TCCTGCCAGG AACAGCGGCT GACCTACCTG CCCCAGCCCT GGGGCAACTG CCGCGCAGAG 
AGTGAGCTCA GGGAGCCTGA GCTTCAGGGC TACTCGGCCT ACAGTGTGTC TGCCTGCCGG 
CTGCGCTGTG AAAAGGAGGC CGTGCTTCAG CGCTGCCACT GCCGGATGGT GCACATGCCA 
GGCAATGAGA CCATCTGCCC ACCAAATATC TACATCGAGT GTGCAGACCA CACACTGGAC 
TCCCTGGGTG GGGGCCCTGA GGGCCCGTGC TTCTGCCCCA CCCCCTGCAA CCTGACACGC 
TATGGGAAAG AGATCTCCAT GGTCAGGATC CCCAACAGGG GCTCAGCCCG GTACCTGGCG 
AGGAAGTACA ACCGCAACGA GACCTACATA CGGGAGAACT TCCTGGTCCT AGATGTCTTC 
TTTGAGGCCC TGACCTCTGA AGCCATGGAG CAGCGAGCAG CCTATGGCCT GTCAGCCCTG 
CTGGGAGACC TCGGGGGACA GATGGGCCTG TTCATTGGGG CCAGCATCCT CACGTTGCTG 
GAGATCCTCG ACTACATCTA TGAGGTGTCC TGGGATCGAC TGAAGCGGGT ATGGAGGCGT 
CCCAAGACCC CCCTGCGGAC CTCCACTGGG GGCATCTCCA CTTTGGGGCT TCAGGAGCTG 
AAGGAACAGA GTCCCTGCCC GAGCCGGGGC CGAGTGGAGG GTGGGGGGGT CAGCAGTCTG 
CTCCCCAATC ACCACCACCC CCACGGTCCC CCAGGAGGTC TCTTTGAAGA TTTTGCTTGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASIC4 ( NM_018674.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_061144.3 Homo sapiens acid sensing ion channel subunit family member 4 (ASIC4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu69428
Clone ID Related Accession (Same CDS sequence)	NM_018674.5
Accession Version	NM_018674.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-01
Organism	Homo sapiens(human)
Product	acid-sensing ion channel 4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC053503.7 and AC009955.4. On or before Aug 9, 2016 this sequence version replaced XM_005246671.2, NM_018674.4. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.200303.1, SRR1803613.203146.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGAGCG GAGCGGCTGG GGCTGCGCGG CGTGGCGGAG CAGCGCTCGC TCCCTCGCTC 
ACTCGCTCGC TCGCAGGGAC ACACGCAGGG GCTGACAGCT GTGCTGGTGC TGATAAGGGA 
AGCCACAAGG AGACGATCGA GGAGAGAGAC AAGCGGCAGC AGAGGCAGCA GCGGCAGAGG 
CAGCACCAGG GCTGCGGAGC TGCTGGGAGT GGGAGTGACT CCCCCACCTC GGGCCCCCAC 
CCTGTCCCTG TCCTCTTCCC GCTTGCCCTG AGTTTAGAAG AGCAGCCGCT GCCACCACTG 
CCACTCGGGA GGGCACCAGG GCTGCTGGCT AGGGAGGGAC AGGGCAGGGA GGCTCTGGCC 
AGTCCCAGCA GCCGGGGACA GATGCCGATC GAGATTGTGT GCAAAATCAA ATTTGCTGAG 
GAGGATGCGA AACCCAAGGA GAAGGAGGCA GGGGATGAGC AGAGCCTCCT CGGGGCTGTT 
GCCCCTGGAG CAGCCCCCCG AGACCTGGCC ACCTTTGCCA GCACCAGCAC CCTGCATGGA 
CTGGGCCGGG CCTGTGGCCC AGGCCCCCAC GGACTGCGCA GAACCCTGTG GGCACTGGCC 
CTACTCACCT CGCTGGCTGC CTTCCTGTAC CAGGCGGCTG GCCTGGCCCG GGGCTACCTG 
ACCCGGCCTC ACCTGGTGGC AATGGACCCC GCTGCCCCAG CCCCAGTGGC GGGCTTCCCG 
GCTGTCACCC TCTGCAATAT CAACCGCTTC CGGCATTCGG CACTCAGCGA TGCCGACATC 
TTCCACCTGG CCAATCTGAC AGGGCTGCCC CCCAAAGACC GGGATGGGCA CCGTGCGGCT 
GGCCTGCGCT ACCCAGAGCC TGACATGGTA GACATCCTCA ACCGCACTGG CCACCAGCTC 
GCCGACATGC TTAAGAGCTG CAACTTCAGT GGGCATCACT GCTCCGCCAG CAACTTCTCT 
GTGGTCTATA CTCGCTATGG GAAGTGTTAC ACCTTCAACG CGGACCCGCG GAGCTCGCTG 
CCCAGCCGGG CAGGGGGCAT GGGCAGTGGC CTGGAGATCA TGCTGGACAT CCAGCAGGAG 
GAGTACCTGC CCATCTGGAG GGAGACAAAT GAGACGTCGT TTGAGGCAGG TATTCGGGTG 
CAGATCCACA GCCAGGAGGA GCCGCCCTAC ATCCACCAGC TGGGGTTCGG GGTGTCCCCA 
GGCTTCCAGA CCTTTGTGTC CTGCCAGGAA CAGCGGCTGA CCTACCTGCC CCAGCCCTGG 
GGCAACTGCC GCGCAGAGAG TGAGCTCAGG GAGCCTGAGC TTCAGGGCTA CTCGGCCTAC 
AGTGTGTCTG CCTGCCGGCT GCGCTGTGAA AAGGAGGCCG TGCTTCAGCG CTGCCACTGC 
CGGATGGTGC ACATGCCAGG CAATGAGACC ATCTGCCCAC CAAATATCTA CATCGAGTGT 
GCAGACCACA CACTGGACTC CCTGGGTGGG GGCCCTGAGG GCCCGTGCTT CTGCCCCACC 
CCCTGCAACC TGACACGCTA TGGGAAAGAG ATCTCCATGG TCAGGATCCC CAACAGGGGC 
TCAGCCCGGT ACCTGGCGAG GAAGTACAAC CGCAACGAGA CCTACATACG GGAGAACTTC 
CTGGTCCTAG ATGTCTTCTT TGAGGCCCTG ACCTCTGAAG CCATGGAGCA GCGAGCAGCC 
TATGGCCTGT CAGCCCTGCT GGGAGACCTC GGGGGACAGA TGGGCCTGTT CATTGGGGCC 
AGCATCCTCA CGTTGCTGGA GATCCTCGAC TACATCTATG AGGTGTCCTG GGATCGACTG 
AAGCGGGTAT GGAGGCGTCC CAAGACCCCC CTGCGGACCT CCACTGGGGG CATCTCCACT 
TTGGGGCTTC AGGAGCTGAA GGAACAGAGT CCCTGCCCGA GCCGGGGCCG AGTGGAGGGT 
GGGGGGGTCA GCAGTCTGCT CCCCAATCAC CACCACCCCC ACGGTCCCCC AGGAGGTCTC 
TTTGAAGATT TTGCTTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061144.3
CDS	175..2175
Translation	MLSGAAGAARRGGAALAPSLTRSLAGTHAGADSCAGADKGSHKETIEERDKRQQRQQRQRQHQGCGAAGSGSDSPTSGPHPVPVLFPLALSLEEQPLPPLPLGRAPGLLAREGQGREALASPSSRGQMPIEIVCKIKFAEEDAKPKEKEAGDEQSLLGAVAPGAAPRDLATFASTSTLHGLGRACGPGPHGLRRTLWALALLTSLAAFLYQAAGLARGYLTRPHLVAMDPAAPAPVAGFPAVTLCNINRFRHSALSDADIFHLANLTGLPPKDRDGHRAAGLRYPEPDMVDILNRTGHQLADMLKSCNFSGHHCSASNFSVVYTRYGKCYTFNADPRSSLPSRAGGMGSGLEIMLDIQQEEYLPIWRETNETSFEAGIRVQIHSQEEPPYIHQLGFGVSPGFQTFVSCQEQRLTYLPQPWGNCRAESELREPELQGYSAYSVSACRLRCEKEAVLQRCHCRMVHMPGNETICPPNIYIECADHTLDSLGGGPEGPCFCPTPCNLTRYGKEISMVRIPNRGSARYLARKYNRNETYIRENFLVLDVFFEALTSEAMEQRAAYGLSALLGDLGGQMGLFIGASILTLLEILDYIYEVSWDRLKRVWRRPKTPLRTSTGGISTLGLQELKEQSPCPSRGRVEGGGVSSLLPNHHHPHGPPGGLFEDFAC

Target ORF information:

RefSeq Version	NM_018674.5
Organism	Homo sapiens(human)
Definition	Homo sapiens acid sensing ion channel subunit family member 4 (ASIC4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018674.5

ORF Insert Sequence:

ATGCTGAGCG GAGCGGCTGG GGCTGCGCGG CGTGGCGGAG CAGCGCTCGC TCCCTCGCTC 
ACTCGCTCGC TCGCAGGGAC ACACGCAGGG GCTGACAGCT GTGCTGGTGC TGATAAGGGA 
AGCCACAAGG AGACGATCGA GGAGAGAGAC AAGCGGCAGC AGAGGCAGCA GCGGCAGAGG 
CAGCACCAGG GCTGCGGAGC TGCTGGGAGT GGGAGTGACT CCCCCACCTC GGGCCCCCAC 
CCTGTCCCTG TCCTCTTCCC GCTTGCCCTG AGTTTAGAAG AGCAGCCGCT GCCACCACTG 
CCACTCGGGA GGGCACCAGG GCTGCTGGCT AGGGAGGGAC AGGGCAGGGA GGCTCTGGCC 
AGTCCCAGCA GCCGGGGACA GATGCCGATC GAGATTGTGT GCAAAATCAA ATTTGCTGAG 
GAGGATGCGA AACCCAAGGA GAAGGAGGCA GGGGATGAGC AGAGCCTCCT CGGGGCTGTT 
GCCCCTGGAG CAGCCCCCCG AGACCTGGCC ACCTTTGCCA GCACCAGCAC CCTGCATGGA 
CTGGGCCGGG CCTGTGGCCC AGGCCCCCAC GGACTGCGCA GAACCCTGTG GGCACTGGCC 
CTACTCACCT CGCTGGCTGC CTTCCTGTAC CAGGCGGCTG GCCTGGCCCG GGGCTACCTG 
ACCCGGCCTC ACCTGGTGGC AATGGACCCC GCTGCCCCAG CCCCAGTGGC GGGCTTCCCG 
GCTGTCACCC TCTGCAATAT CAACCGCTTC CGGCATTCGG CACTCAGCGA TGCCGACATC 
TTCCACCTGG CCAATCTGAC AGGGCTGCCC CCCAAAGACC GGGATGGGCA CCGTGCGGCT 
GGCCTGCGCT ACCCAGAGCC TGACATGGTA GACATCCTCA ACCGCACTGG CCACCAGCTC 
GCCGACATGC TTAAGAGCTG CAACTTCAGT GGGCATCACT GCTCCGCCAG CAACTTCTCT 
GTGGTCTATA CTCGCTATGG GAAGTGTTAC ACCTTCAACG CGGACCCGCG GAGCTCGCTG 
CCCAGCCGGG CAGGGGGCAT GGGCAGTGGC CTGGAGATCA TGCTGGACAT CCAGCAGGAG 
GAGTACCTGC CCATCTGGAG GGAGACAAAT GAGACGTCGT TTGAGGCAGG TATTCGGGTG 
CAGATCCACA GCCAGGAGGA GCCGCCCTAC ATCCACCAGC TGGGGTTCGG GGTGTCCCCA 
GGCTTCCAGA CCTTTGTGTC CTGCCAGGAA CAGCGGCTGA CCTACCTGCC CCAGCCCTGG 
GGCAACTGCC GCGCAGAGAG TGAGCTCAGG GAGCCTGAGC TTCAGGGCTA CTCGGCCTAC 
AGTGTGTCTG CCTGCCGGCT GCGCTGTGAA AAGGAGGCCG TGCTTCAGCG CTGCCACTGC 
CGGATGGTGC ACATGCCAGG CAATGAGACC ATCTGCCCAC CAAATATCTA CATCGAGTGT 
GCAGACCACA CACTGGACTC CCTGGGTGGG GGCCCTGAGG GCCCGTGCTT CTGCCCCACC 
CCCTGCAACC TGACACGCTA TGGGAAAGAG ATCTCCATGG TCAGGATCCC CAACAGGGGC 
TCAGCCCGGT ACCTGGCGAG GAAGTACAAC CGCAACGAGA CCTACATACG GGAGAACTTC 
CTGGTCCTAG ATGTCTTCTT TGAGGCCCTG ACCTCTGAAG CCATGGAGCA GCGAGCAGCC 
TATGGCCTGT CAGCCCTGCT GGGAGACCTC GGGGGACAGA TGGGCCTGTT CATTGGGGCC 
AGCATCCTCA CGTTGCTGGA GATCCTCGAC TACATCTATG AGGTGTCCTG GGATCGACTG 
AAGCGGGTAT GGAGGCGTCC CAAGACCCCC CTGCGGACCT CCACTGGGGG CATCTCCACT 
TTGGGGCTTC AGGAGCTGAA GGAACAGAGT CCCTGCCCGA GCCGGGGCCG AGTGGAGGGT 
GGGGGGGTCA GCAGTCTGCT CCCCAATCAC CACCACCCCC ACGGTCCCCC AGGAGGTCTC 
TTTGAAGATT TTGCTTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Acid-sensing ion channel (ASIC) 4 predominantly localizes to an early endosome-related organelle upon heterologous expression.
Scientific reports518242(2015 Dec)
Schwartz V,Friedrich K,Polleichtner G,Gr?nder S

Regulation of ASIC activity by ASIC4--new insights into ASIC channel function revealed by a yeast two-hybrid assay.
The European journal of neuroscience28(1)74-86(2008 Jul)
Donier E,Rugiero F,Jacob C,Wood JN

Acid-sensing ion channel (ASIC) 4 gene: physical mapping, genomic organisation, and evaluation as a candidate for paroxysmal dystonia.
European journal of human genetics : EJHG9(9)672-6(2001 Sep)
Gr?nder S,Geisler HS,Rainier S,Fink JK

A new member of acid-sensing ion channels from pituitary gland.
Neuroreport11(8)1607-11(2000 Jun)
Gr?nder S,Geissler HS,B?ssler EL,Ruppersberg JP

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ASIC4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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