PRCP cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRCP
Entrez Gene ID	5547
Full Name	prolylcarboxypeptidase
Synonyms	HUMPCP,PCP
General protein information	Preferred Names prolylcarboxypeptidase Names lysosomal Pro-X carboxypeptidase angiotensinase C lysosomal carboxypeptidase C proline carboxypeptidase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q14.1
Summary	This gene encodes a member of the peptidase S28 family of serine exopeptidases. The encoded preproprotein is proteolytically processed to generate the mature lysosomal prolylcarboxypeptidase. This enzyme cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. A pseudogene of this gene has been identified on chromosome 2. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016].
Disorder MIM:	176785

mRNA and Protein(s)

mRNA	Protein	Name
NM_005040.2	NP_005031.1	lysosomal Pro-X carboxypeptidase isoform 1 preproprotein
NM_199418.3	NP_955450.2	lysosomal Pro-X carboxypeptidase isoform 2 precursor
NM_001319214.1	NP_001306143.1	lysosomal Pro-X carboxypeptidase isoform 3
XM_005274093.1	XP_005274150.1	lysosomal Pro-X carboxypeptidase isoform X1
NM_005040.3	NP_005031.1	lysosomal Pro-X carboxypeptidase isoform 1 preproprotein
NM_005040.4	NP_005031.1	lysosomal Pro-X carboxypeptidase isoform 1 preproprotein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PRCP	XP_001175153.1
	PRCP	XP_001096750.1
Mus musculus (house mouse)	Prcp	NP_082519.1
Rattus norvegicus (Norway rat)	Prcp	NP_001099751.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000994	XP_309189.4
Oryza sativa (rice)	Os01g0767100	NP_001044360.1
Xenopus tropicalis (tropical clawed frog)	prcp	NP_001072639.1
Canis lupus familiaris (dog)	PRCP	XP_533994.2
Danio rerio (zebrafish)	prcp	NP_001002694.1
Homo sapiens (human)	PRCP	NP_005031.1
Bos taurus (cattle)	PRCP	NP_001033253.1
Gallus gallus (chicken)	PRCP	XP_425654.3
Drosophila melanogaster (fruit fly)	CG2493	NP_610037.1
Caenorhabditis elegans (roundworm)	pcp-5	NP_498718.1
Arabidopsis thaliana (thale cress)	AT5G65760	NP_201377.2

Gene Ontology
Bibliography

Related articles in PubMed

Identification of novel molecular signatures of IgA nephropathy through an integrative -omics analysis.
Krochmal M, Cisek K, Filip S, Markoska K, Orange C, Zoidakis J, Gakiopoulou C, Spasovski G, Mischak H, Delles C, Vlahou A, Jankowski J
Scientific reports7(1)9091(2017 Aug)

Prolyl carboxypeptidase purified from human placenta: its characterization and identification as an apelin-cleaving enzyme.
Kehoe K, Van Elzen R, Verkerk R, Sim Y, Van der Veken P, Lambeir AM, De Meester I
Biochimica et biophysica acta1864(11)1481-8(2016 11)

Prolyl carboxypeptidase activity decline correlates with severity and short-term outcome in acute ischemic stroke.
Kehoe K, Brouns R, Verkerk R, Engelborghs S, De Deyn PP, Hendriks D, De Meester I
Neurochemical research40(1)81-8(2015 Jan)

Prolylcarboxypeptidase independently activates plasma prekallikrein (fletcher factor).
Wang J, Matafonov A, Madkhali H, Mahdi F, Watson D, Schmaier AH, Gailani D, Shariat-Madar Z
Current molecular medicine14(9)1173-85(2014)

Prolylcarboxypeptidase promotes angiogenesis and vascular repair.
Adams GN, Stavrou EX, Fang C, Merkulova A, Alaiti MA, Nakajima K, Morooka T, Merkulov S, Larusch GA, Simon DI, Jain MK, Schmaier AH
Blood122(8)1522-31(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

For the first time, PRCP is identified as an apelin-cleaving enzyme.
Title: Prolyl carboxypeptidase purified from human placenta: its characterization and identification as an apelin-cleaving enzyme.

The decrease in PRCP levels in the first 24 h after stroke onset is associated with stroke severity and an unfavourable short-term stroke outcome
Title: Prolyl carboxypeptidase activity decline correlates with severity and short-term outcome in acute ischemic stroke.

PRCP1 interacts with plasma kallikrein (PK) at multiple sites for PK activation.
Title: Prolylcarboxypeptidase independently activates plasma prekallikrein (fletcher factor).

The present results indicated PRCP rs7104980 can be considered as a marker for EH and Hap3 GAGCACTAACA (PRCP) and Hap16 TTTA (CMA1) might be associated with essential hypertension in Chinese Han population.
Title: Association of polymorphisms in prolylcarboxypeptidase and chymase genes with essential hypertension in the Chinese Han population.

PRCP regulates cell growth, angiogenesis, and the response to vascular injury
Title: Prolylcarboxypeptidase promotes angiogenesis and vascular repair.

The following PRCP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRCP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23775	NM_005040.4 Latest version!	Homo sapiens prolylcarboxypeptidase (PRCP), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu45424	NM_001319214.1 Latest version!	Homo sapiens prolylcarboxypeptidase (PRCP), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu45424	XM_005274093.1 Latest version!	Homo sapiens prolylcarboxypeptidase (PRCP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu23893	NM_199418.3 Latest version!	Homo sapiens prolylcarboxypeptidase (PRCP), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$300.30 $429.00
View Old Accession Versions >>
OHu23775	NM_005040.2	Homo sapiens prolylcarboxypeptidase (angiotensinase C) (PRCP), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu23775	NM_005040.3	Homo sapiens prolylcarboxypeptidase (PRCP), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRCP ( NM_005040.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005031.1 Homo sapiens prolylcarboxypeptidase (angiotensinase C) (PRCP), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23775
Clone ID Related Accession (Same CDS sequence)	NM_005040.2 , NM_005040.3 , NM_005040.4
Accession Version	NM_005040.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	lysosomal Pro-X carboxypeptidase isoform 1 preproprotein
Comment

ATGGGCCGCC GAGCCCTCCT GCTCCTGCTT CTGTCTTTTC TGGCGCCCTG GGCCACCATA 
GCCCTCCGGC CGGCCTTAAG GGCCCTCGGC AGCCTACACT TGCCAACCAA CCCCACATCC 
CTCCCGGCTG TAGCCAAGAA CTATTCGGTT CTCTACTTCC AACAGAAGGT TGATCATTTT 
GGATTTAATA CTGTGAAAAC TTTTAATCAG CGGTACCTAG TAGCTGATAA ATACTGGAAG 
AAAAATGGTG GATCAATACT TTTCTACACT GGTAATGAAG GGGACATTAT CTGGTTTTGT 
AATAACACGG GGTTCATGTG GGATGTGGCT GAGGAACTGA AAGCTATGTT GGTGTTTGCT 
GAACATCGAT ACTATGGAGA GTCTCTCCCC TTTGGTGACA ACTCATTCAA GGATTCCAGA 
CACTTGAATT TCCTGACATC AGAACAAGCT CTGGCTGATT TTGCAGAGTT AATCAAACAC 
TTGAAAAGAA CAATCCCAGG AGCTGAAAAT CAACCTGTCA TTGCCATAGG AGGCTCCTAT 
GGTGGCATGC TTGCCGCCTG GTTTAGGATG AAATATCCTC ATATGGTAGT TGGAGCTCTT 
GCAGCTTCTG CCCCTATCTG GCAGTTTGAG GATTTAGTAC CTTGTGGTGT ATTTATGAAG 
ATCGTAACTA CAGATTTTAG GAAAAGCGGT CCACATTGTT CAGAGAGCAT CCACAGGTCC 
TGGGATGCCA TTAATCGACT CTCAAATACT GGCAGTGGTT TGCAGTGGCT TACTGGAGCC 
CTTCACTTAT GCAGCCCATT AACTTCTCAG GACATCCAAC ATTTGAAAGA CTGGATCTCT 
GAAACCTGGG TGAATCTGGC AATGGTGGAC TATCCTTATG CCTCTAACTT TTTACAGCCT 
TTGCCTGCTT GGCCTATCAA GGTAGTGTGC CAGTATTTGA AAAATCCCAA TGTATCTGAT 
TCACTGCTGC TGCAGAATAT TTTCCAAGCT CTGAATGTAT ATTACAATTA TTCGGGCCAG 
GTGAAATGCC TGAATATTTC AGAGACAGCA ACTAGCAGTC TGGGAACACT GGGTTGGAGC 
TATCAGGCCT GCACAGAAGT AGTCATGCCC TTTTGTACTA ATGGTGTCGA TGACATGTTT 
GAACCTCACT CATGGAACTT AAAGGAACTT TCTGATGACT GTTTTCAACA GTGGGGTGTG 
AGACCAAGGC CCTCCTGGAT CACTACTATG TATGGAGGCA AAAACATTAG TTCACACACA 
AACATTGTTT TCAGCAATGG TGAACTAGAC CCCTGGTCAG GAGGTGGAGT AACTAAGGAT 
ATCACAGACA CTCTGGTTGC AGTCACCATC TCAGAGGGGG CCCACCACTT AGATCTCCGC 
ACCAAGAATG CCTTGGATCC TATGTCTGTG CTGTTAGCCC GCTCCTTGGA AGTTAGACAT 
ATGAAGAATT GGATCAGAGA TTTCTATGAC AGTGCGGGAA AGCAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_005031.1

Translation

MGRRALLLLLLSFLAPWATIALRPALRALGSLHLPTNPTSLPAVAKNYSVLYFQQKVDHFGFNTVKTFNQRYLVADKYWKKNGGSILFYTGNEGDIIWFCNNTGFMWDVAEELKAMLVFAEHRYYGESLPFGDNSFKDSRHLNFLTSEQALADFAELIKHLKRTIPGAENQPVIAIGGSYGGMLAAWFRMKYPHMVVGALAASAPIWQFEDLVPCGVFMKIVTTDFRKSGPHCSESIHRSWDAINRLSNTGSGLQWLTGALHLCSPLTSQDIQHLKDWISETWVNLAMVDYPYASNFLQPLPAWPIKVVCQYLKNPNVSDSLLLQNIFQALNVYYNYSGQVKCLNISETATSSLGTLGWSYQACTEVVMPFCTNGVDDMFEPHSWNLKELSDDCFQQWGVRPRPSWITTMYGGKNISSHTNIVFSNGELDPWSGGGVTKDITDTLVAVTISEGAHHLDLRTKNALDPMSVLLARSLEVRHMKNWIRDFYDSAGKQH

Target ORF information:

RefSeq Version	NM_005040.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens prolylcarboxypeptidase (angiotensinase C) (PRCP), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005040.2

ORF Insert Sequence:

ATGGGCCGCC GAGCCCTCCT GCTCCTGCTT CTGTCTTTTC TGGCGCCCTG GGCCACCATA 
GCCCTCCGGC CGGCCTTAAG GGCCCTCGGC AGCCTACACT TGCCAACCAA CCCCACATCC 
CTCCCGGCTG TAGCCAAGAA CTATTCGGTT CTCTACTTCC AACAGAAGGT TGATCATTTT 
GGATTTAATA CTGTGAAAAC TTTTAATCAG CGGTACCTAG TAGCTGATAA ATACTGGAAG 
AAAAATGGTG GATCAATACT TTTCTACACT GGTAATGAAG GGGACATTAT CTGGTTTTGT 
AATAACACGG GGTTCATGTG GGATGTGGCT GAGGAACTGA AAGCTATGTT GGTGTTTGCT 
GAACATCGAT ACTATGGAGA GTCTCTCCCC TTTGGTGACA ACTCATTCAA GGATTCCAGA 
CACTTGAATT TCCTGACATC AGAACAAGCT CTGGCTGATT TTGCAGAGTT AATCAAACAC 
TTGAAAAGAA CAATCCCAGG AGCTGAAAAT CAACCTGTCA TTGCCATAGG AGGCTCCTAT 
GGTGGCATGC TTGCCGCCTG GTTTAGGATG AAATATCCTC ATATGGTAGT TGGAGCTCTT 
GCAGCTTCTG CCCCTATCTG GCAGTTTGAG GATTTAGTAC CTTGTGGTGT ATTTATGAAG 
ATCGTAACTA CAGATTTTAG GAAAAGCGGT CCACATTGTT CAGAGAGCAT CCACAGGTCC 
TGGGATGCCA TTAATCGACT CTCAAATACT GGCAGTGGTT TGCAGTGGCT TACTGGAGCC 
CTTCACTTAT GCAGCCCATT AACTTCTCAG GACATCCAAC ATTTGAAAGA CTGGATCTCT 
GAAACCTGGG TGAATCTGGC AATGGTGGAC TATCCTTATG CCTCTAACTT TTTACAGCCT 
TTGCCTGCTT GGCCTATCAA GGTAGTGTGC CAGTATTTGA AAAATCCCAA TGTATCTGAT 
TCACTGCTGC TGCAGAATAT TTTCCAAGCT CTGAATGTAT ATTACAATTA TTCGGGCCAG 
GTGAAATGCC TGAATATTTC AGAGACAGCA ACTAGCAGTC TGGGAACACT GGGTTGGAGC 
TATCAGGCCT GCACAGAAGT AGTCATGCCC TTTTGTACTA ATGGTGTCGA TGACATGTTT 
GAACCTCACT CATGGAACTT AAAGGAACTT TCTGATGACT GTTTTCAACA GTGGGGTGTG 
AGACCAAGGC CCTCCTGGAT CACTACTATG TATGGAGGCA AAAACATTAG TTCACACACA 
AACATTGTTT TCAGCAATGG TGAACTAGAC CCCTGGTCAG GAGGTGGAGT AACTAAGGAT 
ATCACAGACA CTCTGGTTGC AGTCACCATC TCAGAGGGGG CCCACCACTT AGATCTCCGC 
ACCAAGAATG CCTTGGATCC TATGTCTGTG CTGTTAGCCC GCTCCTTGGA AGTTAGACAT 
ATGAAGAATT GGATCAGAGA TTTCTATGAC AGTGCGGGAA AGCAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRCP ( NM_005040.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005031.1 Homo sapiens prolylcarboxypeptidase (PRCP), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23775
Clone ID Related Accession (Same CDS sequence)	NM_005040.2 , NM_005040.3 , NM_005040.4
Accession Version	NM_005040.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	lysosomal Pro-X carboxypeptidase isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB444448.1, BI827978.1, BC001500.2, AA477618.1, BE154586.1, CB154314.1, AP001646.5 and AW627417.1. On Jan 30, 2016 this sequence version replaced NM_005040.2. Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.257561.1, SRR1660803.240032.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCCGCC GAGCCCTCCT GCTCCTGCTT CTGTCTTTTC TGGCGCCCTG GGCCACCATA 
GCCCTCCGGC CGGCCTTAAG GGCCCTCGGC AGCCTACACT TGCCAACCAA CCCCACATCC 
CTCCCGGCTG TAGCCAAGAA CTATTCGGTT CTCTACTTCC AACAGAAGGT TGATCATTTT 
GGATTTAATA CTGTGAAAAC TTTTAATCAG CGGTACCTAG TAGCTGATAA ATACTGGAAG 
AAAAATGGTG GATCAATACT TTTCTACACT GGTAATGAAG GGGACATTAT CTGGTTTTGT 
AATAACACGG GGTTCATGTG GGATGTGGCT GAGGAACTGA AAGCTATGTT GGTGTTTGCT 
GAACATCGAT ACTATGGAGA GTCTCTCCCC TTTGGTGACA ACTCATTCAA GGATTCCAGA 
CACTTGAATT TCCTGACATC AGAACAAGCT CTGGCTGATT TTGCAGAGTT AATCAAACAC 
TTGAAAAGAA CAATCCCAGG AGCTGAAAAT CAACCTGTCA TTGCCATAGG AGGCTCCTAT 
GGTGGCATGC TTGCCGCCTG GTTTAGGATG AAATATCCTC ATATGGTAGT TGGAGCTCTT 
GCAGCTTCTG CCCCTATCTG GCAGTTTGAG GATTTAGTAC CTTGTGGTGT ATTTATGAAG 
ATCGTAACTA CAGATTTTAG GAAAAGCGGT CCACATTGTT CAGAGAGCAT CCACAGGTCC 
TGGGATGCCA TTAATCGACT CTCAAATACT GGCAGTGGTT TGCAGTGGCT TACTGGAGCC 
CTTCACTTAT GCAGCCCATT AACTTCTCAG GACATCCAAC ATTTGAAAGA CTGGATCTCT 
GAAACCTGGG TGAATCTGGC AATGGTGGAC TATCCTTATG CCTCTAACTT TTTACAGCCT 
TTGCCTGCTT GGCCTATCAA GGTAGTGTGC CAGTATTTGA AAAATCCCAA TGTATCTGAT 
TCACTGCTGC TGCAGAATAT TTTCCAAGCT CTGAATGTAT ATTACAATTA TTCGGGCCAG 
GTGAAATGCC TGAATATTTC AGAGACAGCA ACTAGCAGTC TGGGAACACT GGGTTGGAGC 
TATCAGGCCT GCACAGAAGT AGTCATGCCC TTTTGTACTA ATGGTGTCGA TGACATGTTT 
GAACCTCACT CATGGAACTT AAAGGAACTT TCTGATGACT GTTTTCAACA GTGGGGTGTG 
AGACCAAGGC CCTCCTGGAT CACTACTATG TATGGAGGCA AAAACATTAG TTCACACACA 
AACATTGTTT TCAGCAATGG TGAACTAGAC CCCTGGTCAG GAGGTGGAGT AACTAAGGAT 
ATCACAGACA CTCTGGTTGC AGTCACCATC TCAGAGGGGG CCCACCACTT AGATCTCCGC 
ACCAAGAATG CCTTGGATCC TATGTCTGTG CTGTTAGCCC GCTCCTTGGA AGTTAGACAT 
ATGAAGAATT GGATCAGAGA TTTCTATGAC AGTGCGGGAA AGCAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005031.1
CDS	202..1692
Translation	MGRRALLLLLLSFLAPWATIALRPALRALGSLHLPTNPTSLPAVAKNYSVLYFQQKVDHFGFNTVKTFNQRYLVADKYWKKNGGSILFYTGNEGDIIWFCNNTGFMWDVAEELKAMLVFAEHRYYGESLPFGDNSFKDSRHLNFLTSEQALADFAELIKHLKRTIPGAENQPVIAIGGSYGGMLAAWFRMKYPHMVVGALAASAPIWQFEDLVPCGVFMKIVTTDFRKSGPHCSESIHRSWDAINRLSNTGSGLQWLTGALHLCSPLTSQDIQHLKDWISETWVNLAMVDYPYASNFLQPLPAWPIKVVCQYLKNPNVSDSLLLQNIFQALNVYYNYSGQVKCLNISETATSSLGTLGWSYQACTEVVMPFCTNGVDDMFEPHSWNLKELSDDCFQQWGVRPRPSWITTMYGGKNISSHTNIVFSNGELDPWSGGGVTKDITDTLVAVTISEGAHHLDLRTKNALDPMSVLLARSLEVRHMKNWIRDFYDSAGKQH

Target ORF information:

RefSeq Version	NM_005040.3
Organism	Homo sapiens(human)
Definition	Homo sapiens prolylcarboxypeptidase (PRCP), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005040.3

ORF Insert Sequence:

ATGGGCCGCC GAGCCCTCCT GCTCCTGCTT CTGTCTTTTC TGGCGCCCTG GGCCACCATA 
GCCCTCCGGC CGGCCTTAAG GGCCCTCGGC AGCCTACACT TGCCAACCAA CCCCACATCC 
CTCCCGGCTG TAGCCAAGAA CTATTCGGTT CTCTACTTCC AACAGAAGGT TGATCATTTT 
GGATTTAATA CTGTGAAAAC TTTTAATCAG CGGTACCTAG TAGCTGATAA ATACTGGAAG 
AAAAATGGTG GATCAATACT TTTCTACACT GGTAATGAAG GGGACATTAT CTGGTTTTGT 
AATAACACGG GGTTCATGTG GGATGTGGCT GAGGAACTGA AAGCTATGTT GGTGTTTGCT 
GAACATCGAT ACTATGGAGA GTCTCTCCCC TTTGGTGACA ACTCATTCAA GGATTCCAGA 
CACTTGAATT TCCTGACATC AGAACAAGCT CTGGCTGATT TTGCAGAGTT AATCAAACAC 
TTGAAAAGAA CAATCCCAGG AGCTGAAAAT CAACCTGTCA TTGCCATAGG AGGCTCCTAT 
GGTGGCATGC TTGCCGCCTG GTTTAGGATG AAATATCCTC ATATGGTAGT TGGAGCTCTT 
GCAGCTTCTG CCCCTATCTG GCAGTTTGAG GATTTAGTAC CTTGTGGTGT ATTTATGAAG 
ATCGTAACTA CAGATTTTAG GAAAAGCGGT CCACATTGTT CAGAGAGCAT CCACAGGTCC 
TGGGATGCCA TTAATCGACT CTCAAATACT GGCAGTGGTT TGCAGTGGCT TACTGGAGCC 
CTTCACTTAT GCAGCCCATT AACTTCTCAG GACATCCAAC ATTTGAAAGA CTGGATCTCT 
GAAACCTGGG TGAATCTGGC AATGGTGGAC TATCCTTATG CCTCTAACTT TTTACAGCCT 
TTGCCTGCTT GGCCTATCAA GGTAGTGTGC CAGTATTTGA AAAATCCCAA TGTATCTGAT 
TCACTGCTGC TGCAGAATAT TTTCCAAGCT CTGAATGTAT ATTACAATTA TTCGGGCCAG 
GTGAAATGCC TGAATATTTC AGAGACAGCA ACTAGCAGTC TGGGAACACT GGGTTGGAGC 
TATCAGGCCT GCACAGAAGT AGTCATGCCC TTTTGTACTA ATGGTGTCGA TGACATGTTT 
GAACCTCACT CATGGAACTT AAAGGAACTT TCTGATGACT GTTTTCAACA GTGGGGTGTG 
AGACCAAGGC CCTCCTGGAT CACTACTATG TATGGAGGCA AAAACATTAG TTCACACACA 
AACATTGTTT TCAGCAATGG TGAACTAGAC CCCTGGTCAG GAGGTGGAGT AACTAAGGAT 
ATCACAGACA CTCTGGTTGC AGTCACCATC TCAGAGGGGG CCCACCACTT AGATCTCCGC 
ACCAAGAATG CCTTGGATCC TATGTCTGTG CTGTTAGCCC GCTCCTTGGA AGTTAGACAT 
ATGAAGAATT GGATCAGAGA TTTCTATGAC AGTGCGGGAA AGCAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRCP ( NM_005040.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005031.1 Homo sapiens prolylcarboxypeptidase (PRCP), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23775
Clone ID Related Accession (Same CDS sequence)	NM_005040.2 , NM_005040.3 , NM_005040.4
Accession Version	NM_005040.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	lysosomal Pro-X carboxypeptidase isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI827978.1, BC001500.2, AA477618.1, BE154586.1, CB154314.1, AP001646.5 and AW627417.1. On Nov 23, 2018 this sequence version replaced NM_005040.3. Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.158416.1, SRR1803612.257561.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000313010.8/ ENSP00000317362.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCCGCC GAGCCCTCCT GCTCCTGCTT CTGTCTTTTC TGGCGCCCTG GGCCACCATA 
GCCCTCCGGC CGGCCTTAAG GGCCCTCGGC AGCCTACACT TGCCAACCAA CCCCACATCC 
CTCCCGGCTG TAGCCAAGAA CTATTCGGTT CTCTACTTCC AACAGAAGGT TGATCATTTT 
GGATTTAATA CTGTGAAAAC TTTTAATCAG CGGTACCTAG TAGCTGATAA ATACTGGAAG 
AAAAATGGTG GATCAATACT TTTCTACACT GGTAATGAAG GGGACATTAT CTGGTTTTGT 
AATAACACGG GGTTCATGTG GGATGTGGCT GAGGAACTGA AAGCTATGTT GGTGTTTGCT 
GAACATCGAT ACTATGGAGA GTCTCTCCCC TTTGGTGACA ACTCATTCAA GGATTCCAGA 
CACTTGAATT TCCTGACATC AGAACAAGCT CTGGCTGATT TTGCAGAGTT AATCAAACAC 
TTGAAAAGAA CAATCCCAGG AGCTGAAAAT CAACCTGTCA TTGCCATAGG AGGCTCCTAT 
GGTGGCATGC TTGCCGCCTG GTTTAGGATG AAATATCCTC ATATGGTAGT TGGAGCTCTT 
GCAGCTTCTG CCCCTATCTG GCAGTTTGAG GATTTAGTAC CTTGTGGTGT ATTTATGAAG 
ATCGTAACTA CAGATTTTAG GAAAAGCGGT CCACATTGTT CAGAGAGCAT CCACAGGTCC 
TGGGATGCCA TTAATCGACT CTCAAATACT GGCAGTGGTT TGCAGTGGCT TACTGGAGCC 
CTTCACTTAT GCAGCCCATT AACTTCTCAG GACATCCAAC ATTTGAAAGA CTGGATCTCT 
GAAACCTGGG TGAATCTGGC AATGGTGGAC TATCCTTATG CCTCTAACTT TTTACAGCCT 
TTGCCTGCTT GGCCTATCAA GGTAGTGTGC CAGTATTTGA AAAATCCCAA TGTATCTGAT 
TCACTGCTGC TGCAGAATAT TTTCCAAGCT CTGAATGTAT ATTACAATTA TTCGGGCCAG 
GTGAAATGCC TGAATATTTC AGAGACAGCA ACTAGCAGTC TGGGAACACT GGGTTGGAGC 
TATCAGGCCT GCACAGAAGT AGTCATGCCC TTTTGTACTA ATGGTGTCGA TGACATGTTT 
GAACCTCACT CATGGAACTT AAAGGAACTT TCTGATGACT GTTTTCAACA GTGGGGTGTG 
AGACCAAGGC CCTCCTGGAT CACTACTATG TATGGAGGCA AAAACATTAG TTCACACACA 
AACATTGTTT TCAGCAATGG TGAACTAGAC CCCTGGTCAG GAGGTGGAGT AACTAAGGAT 
ATCACAGACA CTCTGGTTGC AGTCACCATC TCAGAGGGGG CCCACCACTT AGATCTCCGC 
ACCAAGAATG CCTTGGATCC TATGTCTGTG CTGTTAGCCC GCTCCTTGGA AGTTAGACAT 
ATGAAGAATT GGATCAGAGA TTTCTATGAC AGTGCGGGAA AGCAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005031.1
CDS	29..1519
Translation	MGRRALLLLLLSFLAPWATIALRPALRALGSLHLPTNPTSLPAVAKNYSVLYFQQKVDHFGFNTVKTFNQRYLVADKYWKKNGGSILFYTGNEGDIIWFCNNTGFMWDVAEELKAMLVFAEHRYYGESLPFGDNSFKDSRHLNFLTSEQALADFAELIKHLKRTIPGAENQPVIAIGGSYGGMLAAWFRMKYPHMVVGALAASAPIWQFEDLVPCGVFMKIVTTDFRKSGPHCSESIHRSWDAINRLSNTGSGLQWLTGALHLCSPLTSQDIQHLKDWISETWVNLAMVDYPYASNFLQPLPAWPIKVVCQYLKNPNVSDSLLLQNIFQALNVYYNYSGQVKCLNISETATSSLGTLGWSYQACTEVVMPFCTNGVDDMFEPHSWNLKELSDDCFQQWGVRPRPSWITTMYGGKNISSHTNIVFSNGELDPWSGGGVTKDITDTLVAVTISEGAHHLDLRTKNALDPMSVLLARSLEVRHMKNWIRDFYDSAGKQH

Target ORF information:

RefSeq Version	NM_005040.4
Organism	Homo sapiens(human)
Definition	Homo sapiens prolylcarboxypeptidase (PRCP), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005040.4

ORF Insert Sequence:

ATGGGCCGCC GAGCCCTCCT GCTCCTGCTT CTGTCTTTTC TGGCGCCCTG GGCCACCATA 
GCCCTCCGGC CGGCCTTAAG GGCCCTCGGC AGCCTACACT TGCCAACCAA CCCCACATCC 
CTCCCGGCTG TAGCCAAGAA CTATTCGGTT CTCTACTTCC AACAGAAGGT TGATCATTTT 
GGATTTAATA CTGTGAAAAC TTTTAATCAG CGGTACCTAG TAGCTGATAA ATACTGGAAG 
AAAAATGGTG GATCAATACT TTTCTACACT GGTAATGAAG GGGACATTAT CTGGTTTTGT 
AATAACACGG GGTTCATGTG GGATGTGGCT GAGGAACTGA AAGCTATGTT GGTGTTTGCT 
GAACATCGAT ACTATGGAGA GTCTCTCCCC TTTGGTGACA ACTCATTCAA GGATTCCAGA 
CACTTGAATT TCCTGACATC AGAACAAGCT CTGGCTGATT TTGCAGAGTT AATCAAACAC 
TTGAAAAGAA CAATCCCAGG AGCTGAAAAT CAACCTGTCA TTGCCATAGG AGGCTCCTAT 
GGTGGCATGC TTGCCGCCTG GTTTAGGATG AAATATCCTC ATATGGTAGT TGGAGCTCTT 
GCAGCTTCTG CCCCTATCTG GCAGTTTGAG GATTTAGTAC CTTGTGGTGT ATTTATGAAG 
ATCGTAACTA CAGATTTTAG GAAAAGCGGT CCACATTGTT CAGAGAGCAT CCACAGGTCC 
TGGGATGCCA TTAATCGACT CTCAAATACT GGCAGTGGTT TGCAGTGGCT TACTGGAGCC 
CTTCACTTAT GCAGCCCATT AACTTCTCAG GACATCCAAC ATTTGAAAGA CTGGATCTCT 
GAAACCTGGG TGAATCTGGC AATGGTGGAC TATCCTTATG CCTCTAACTT TTTACAGCCT 
TTGCCTGCTT GGCCTATCAA GGTAGTGTGC CAGTATTTGA AAAATCCCAA TGTATCTGAT 
TCACTGCTGC TGCAGAATAT TTTCCAAGCT CTGAATGTAT ATTACAATTA TTCGGGCCAG 
GTGAAATGCC TGAATATTTC AGAGACAGCA ACTAGCAGTC TGGGAACACT GGGTTGGAGC 
TATCAGGCCT GCACAGAAGT AGTCATGCCC TTTTGTACTA ATGGTGTCGA TGACATGTTT 
GAACCTCACT CATGGAACTT AAAGGAACTT TCTGATGACT GTTTTCAACA GTGGGGTGTG 
AGACCAAGGC CCTCCTGGAT CACTACTATG TATGGAGGCA AAAACATTAG TTCACACACA 
AACATTGTTT TCAGCAATGG TGAACTAGAC CCCTGGTCAG GAGGTGGAGT AACTAAGGAT 
ATCACAGACA CTCTGGTTGC AGTCACCATC TCAGAGGGGG CCCACCACTT AGATCTCCGC 
ACCAAGAATG CCTTGGATCC TATGTCTGTG CTGTTAGCCC GCTCCTTGGA AGTTAGACAT 
ATGAAGAATT GGATCAGAGA TTTCTATGAC AGTGCGGGAA AGCAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRCP ( NM_001319214.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001306143.1 Homo sapiens prolylcarboxypeptidase (PRCP), transcript variant 3, mRNA.

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CloneID	OHu45424
Clone ID Related Accession (Same CDS sequence)	NM_001319214.1 , XM_005274093.1
Accession Version	NM_001319214.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	lysosomal Pro-X carboxypeptidase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB028823.1, AK302386.1, BC001500.2, AA477618.1, BE154586.1, CB154314.1, AP001646.5 and AW627417.1. Transcript Variant: This variant (3) differs in the the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon compared to variant 1. The encoded isoform (3) has a shorter N-terminus and lacks a predicted signal peptide compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302386.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGATG TGGCTGAGGA ACTGAAAGCT ATGTTGGTGT TTGCTGAACA TCGATACTAT 
GGAGAGTCTC TCCCCTTTGG TGACAACTCA TTCAAGGATT CCAGACACTT GAATTTCCTG 
ACATCAGAAC AAGCTCTGGC TGATTTTGCA GAGTTAATCA AACACTTGAA AAGAACAATC 
CCAGGAGCTG AAAATCAACC TGTCATTGCC ATAGGAGGCT CCTATGGTGG CATGCTTGCC 
GCCTGGTTTA GGATGAAATA TCCTCATATG GTAGTTGGAG CTCTTGCAGC TTCTGCCCCT 
ATCTGGCAGT TTGAGGATTT AGTACCTTGT GGTGTATTTA TGAAGATCGT AACTACAGAT 
TTTAGGAAAA GCGGTCCACA TTGTTCAGAG AGCATCCACA GGTCCTGGGA TGCCATTAAT 
CGACTCTCAA ATACTGGCAG TGGTTTGCAG TGGCTTACTG GAGCCCTTCA CTTATGCAGC 
CCATTAACTT CTCAGGACAT CCAACATTTG AAAGACTGGA TCTCTGAAAC CTGGGTGAAT 
CTGGCAATGG TGGACTATCC TTATGCCTCT AACTTTTTAC AGCCTTTGCC TGCTTGGCCT 
ATCAAGGTAG TGTGCCAGTA TTTGAAAAAT CCCAATGTAT CTGATTCACT GCTGCTGCAG 
AATATTTTCC AAGCTCTGAA TGTATATTAC AATTATTCGG GCCAGGTGAA ATGCCTGAAT 
ATTTCAGAGA CAGCAACTAG CAGTCTGGGA ACACTGGGTT GGAGCTATCA GGCCTGCACA 
GAAGTAGTCA TGCCCTTTTG TACTAATGGT GTCGATGACA TGTTTGAACC TCACTCATGG 
AACTTAAAGG AACTTTCTGA TGACTGTTTT CAACAGTGGG GTGTGAGACC AAGGCCCTCC 
TGGATCACTA CTATGTATGG AGGCAAAAAC ATTAGTTCAC ACACAAACAT TGTTTTCAGC 
AATGGTGAAC TAGACCCCTG GTCAGGAGGT GGAGTAACTA AGGATATCAC AGACACTCTG 
GTTGCAGTCA CCATCTCAGA GGGGGCCCAC CACTTAGATC TCCGCACCAA GAATGCCTTG 
GATCCTATGT CTGTGCTGTT AGCCCGCTCC TTGGAAGTTA GACATATGAA GAATTGGATC 
AGAGATTTCT ATGACAGTGC GGGAAAGCAG CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001306143.1
CDS	155..1330
Translation	MWDVAEELKAMLVFAEHRYYGESLPFGDNSFKDSRHLNFLTSEQALADFAELIKHLKRTIPGAENQPVIAIGGSYGGMLAAWFRMKYPHMVVGALAASAPIWQFEDLVPCGVFMKIVTTDFRKSGPHCSESIHRSWDAINRLSNTGSGLQWLTGALHLCSPLTSQDIQHLKDWISETWVNLAMVDYPYASNFLQPLPAWPIKVVCQYLKNPNVSDSLLLQNIFQALNVYYNYSGQVKCLNISETATSSLGTLGWSYQACTEVVMPFCTNGVDDMFEPHSWNLKELSDDCFQQWGVRPRPSWITTMYGGKNISSHTNIVFSNGELDPWSGGGVTKDITDTLVAVTISEGAHHLDLRTKNALDPMSVLLARSLEVRHMKNWIRDFYDSAGKQH

Target ORF information:

RefSeq Version	NM_001319214.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prolylcarboxypeptidase (PRCP), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001319214.1

ORF Insert Sequence:

ATGTGGGATG TGGCTGAGGA ACTGAAAGCT ATGTTGGTGT TTGCTGAACA TCGATACTAT 
GGAGAGTCTC TCCCCTTTGG TGACAACTCA TTCAAGGATT CCAGACACTT GAATTTCCTG 
ACATCAGAAC AAGCTCTGGC TGATTTTGCA GAGTTAATCA AACACTTGAA AAGAACAATC 
CCAGGAGCTG AAAATCAACC TGTCATTGCC ATAGGAGGCT CCTATGGTGG CATGCTTGCC 
GCCTGGTTTA GGATGAAATA TCCTCATATG GTAGTTGGAG CTCTTGCAGC TTCTGCCCCT 
ATCTGGCAGT TTGAGGATTT AGTACCTTGT GGTGTATTTA TGAAGATCGT AACTACAGAT 
TTTAGGAAAA GCGGTCCACA TTGTTCAGAG AGCATCCACA GGTCCTGGGA TGCCATTAAT 
CGACTCTCAA ATACTGGCAG TGGTTTGCAG TGGCTTACTG GAGCCCTTCA CTTATGCAGC 
CCATTAACTT CTCAGGACAT CCAACATTTG AAAGACTGGA TCTCTGAAAC CTGGGTGAAT 
CTGGCAATGG TGGACTATCC TTATGCCTCT AACTTTTTAC AGCCTTTGCC TGCTTGGCCT 
ATCAAGGTAG TGTGCCAGTA TTTGAAAAAT CCCAATGTAT CTGATTCACT GCTGCTGCAG 
AATATTTTCC AAGCTCTGAA TGTATATTAC AATTATTCGG GCCAGGTGAA ATGCCTGAAT 
ATTTCAGAGA CAGCAACTAG CAGTCTGGGA ACACTGGGTT GGAGCTATCA GGCCTGCACA 
GAAGTAGTCA TGCCCTTTTG TACTAATGGT GTCGATGACA TGTTTGAACC TCACTCATGG 
AACTTAAAGG AACTTTCTGA TGACTGTTTT CAACAGTGGG GTGTGAGACC AAGGCCCTCC 
TGGATCACTA CTATGTATGG AGGCAAAAAC ATTAGTTCAC ACACAAACAT TGTTTTCAGC 
AATGGTGAAC TAGACCCCTG GTCAGGAGGT GGAGTAACTA AGGATATCAC AGACACTCTG 
GTTGCAGTCA CCATCTCAGA GGGGGCCCAC CACTTAGATC TCCGCACCAA GAATGCCTTG 
GATCCTATGT CTGTGCTGTT AGCCCGCTCC TTGGAAGTTA GACATATGAA GAATTGGATC 
AGAGATTTCT ATGACAGTGC GGGAAAGCAG CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRCP ( XM_005274093.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005274150.1 Homo sapiens prolylcarboxypeptidase (PRCP), transcript variant X1, mRNA.

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CloneID	OHu45424
Clone ID Related Accession (Same CDS sequence)	NM_001319214.1 , XM_005274093.1
Accession Version	XM_005274093.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	lysosomal Pro-X carboxypeptidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGATG TGGCTGAGGA ACTGAAAGCT ATGTTGGTGT TTGCTGAACA TCGATACTAT 
GGAGAGTCTC TCCCCTTTGG TGACAACTCA TTCAAGGATT CCAGACACTT GAATTTCCTG 
ACATCAGAAC AAGCTCTGGC TGATTTTGCA GAGTTAATCA AACACTTGAA AAGAACAATC 
CCAGGAGCTG AAAATCAACC TGTCATTGCC ATAGGAGGCT CCTATGGTGG CATGCTTGCC 
GCCTGGTTTA GGATGAAATA TCCTCATATG GTAGTTGGAG CTCTTGCAGC TTCTGCCCCT 
ATCTGGCAGT TTGAGGATTT AGTACCTTGT GGTGTATTTA TGAAGATCGT AACTACAGAT 
TTTAGGAAAA GCGGTCCACA TTGTTCAGAG AGCATCCACA GGTCCTGGGA TGCCATTAAT 
CGACTCTCAA ATACTGGCAG TGGTTTGCAG TGGCTTACTG GAGCCCTTCA CTTATGCAGC 
CCATTAACTT CTCAGGACAT CCAACATTTG AAAGACTGGA TCTCTGAAAC CTGGGTGAAT 
CTGGCAATGG TGGACTATCC TTATGCCTCT AACTTTTTAC AGCCTTTGCC TGCTTGGCCT 
ATCAAGGTAG TGTGCCAGTA TTTGAAAAAT CCCAATGTAT CTGATTCACT GCTGCTGCAG 
AATATTTTCC AAGCTCTGAA TGTATATTAC AATTATTCGG GCCAGGTGAA ATGCCTGAAT 
ATTTCAGAGA CAGCAACTAG CAGTCTGGGA ACACTGGGTT GGAGCTATCA GGCCTGCACA 
GAAGTAGTCA TGCCCTTTTG TACTAATGGT GTCGATGACA TGTTTGAACC TCACTCATGG 
AACTTAAAGG AACTTTCTGA TGACTGTTTT CAACAGTGGG GTGTGAGACC AAGGCCCTCC 
TGGATCACTA CTATGTATGG AGGCAAAAAC ATTAGTTCAC ACACAAACAT TGTTTTCAGC 
AATGGTGAAC TAGACCCCTG GTCAGGAGGT GGAGTAACTA AGGATATCAC AGACACTCTG 
GTTGCAGTCA CCATCTCAGA GGGGGCCCAC CACTTAGATC TCCGCACCAA GAATGCCTTG 
GATCCTATGT CTGTGCTGTT AGCCCGCTCC TTGGAAGTTA GACATATGAA GAATTGGATC 
AGAGATTTCT ATGACAGTGC GGGAAAGCAG CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005274150.1
CDS	247..1422
Translation	MWDVAEELKAMLVFAEHRYYGESLPFGDNSFKDSRHLNFLTSEQALADFAELIKHLKRTIPGAENQPVIAIGGSYGGMLAAWFRMKYPHMVVGALAASAPIWQFEDLVPCGVFMKIVTTDFRKSGPHCSESIHRSWDAINRLSNTGSGLQWLTGALHLCSPLTSQDIQHLKDWISETWVNLAMVDYPYASNFLQPLPAWPIKVVCQYLKNPNVSDSLLLQNIFQALNVYYNYSGQVKCLNISETATSSLGTLGWSYQACTEVVMPFCTNGVDDMFEPHSWNLKELSDDCFQQWGVRPRPSWITTMYGGKNISSHTNIVFSNGELDPWSGGGVTKDITDTLVAVTISEGAHHLDLRTKNALDPMSVLLARSLEVRHMKNWIRDFYDSAGKQH

Target ORF information:

RefSeq Version	XM_005274093.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prolylcarboxypeptidase (PRCP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005274093.1

ORF Insert Sequence:

ATGTGGGATG TGGCTGAGGA ACTGAAAGCT ATGTTGGTGT TTGCTGAACA TCGATACTAT 
GGAGAGTCTC TCCCCTTTGG TGACAACTCA TTCAAGGATT CCAGACACTT GAATTTCCTG 
ACATCAGAAC AAGCTCTGGC TGATTTTGCA GAGTTAATCA AACACTTGAA AAGAACAATC 
CCAGGAGCTG AAAATCAACC TGTCATTGCC ATAGGAGGCT CCTATGGTGG CATGCTTGCC 
GCCTGGTTTA GGATGAAATA TCCTCATATG GTAGTTGGAG CTCTTGCAGC TTCTGCCCCT 
ATCTGGCAGT TTGAGGATTT AGTACCTTGT GGTGTATTTA TGAAGATCGT AACTACAGAT 
TTTAGGAAAA GCGGTCCACA TTGTTCAGAG AGCATCCACA GGTCCTGGGA TGCCATTAAT 
CGACTCTCAA ATACTGGCAG TGGTTTGCAG TGGCTTACTG GAGCCCTTCA CTTATGCAGC 
CCATTAACTT CTCAGGACAT CCAACATTTG AAAGACTGGA TCTCTGAAAC CTGGGTGAAT 
CTGGCAATGG TGGACTATCC TTATGCCTCT AACTTTTTAC AGCCTTTGCC TGCTTGGCCT 
ATCAAGGTAG TGTGCCAGTA TTTGAAAAAT CCCAATGTAT CTGATTCACT GCTGCTGCAG 
AATATTTTCC AAGCTCTGAA TGTATATTAC AATTATTCGG GCCAGGTGAA ATGCCTGAAT 
ATTTCAGAGA CAGCAACTAG CAGTCTGGGA ACACTGGGTT GGAGCTATCA GGCCTGCACA 
GAAGTAGTCA TGCCCTTTTG TACTAATGGT GTCGATGACA TGTTTGAACC TCACTCATGG 
AACTTAAAGG AACTTTCTGA TGACTGTTTT CAACAGTGGG GTGTGAGACC AAGGCCCTCC 
TGGATCACTA CTATGTATGG AGGCAAAAAC ATTAGTTCAC ACACAAACAT TGTTTTCAGC 
AATGGTGAAC TAGACCCCTG GTCAGGAGGT GGAGTAACTA AGGATATCAC AGACACTCTG 
GTTGCAGTCA CCATCTCAGA GGGGGCCCAC CACTTAGATC TCCGCACCAA GAATGCCTTG 
GATCCTATGT CTGTGCTGTT AGCCCGCTCC TTGGAAGTTA GACATATGAA GAATTGGATC 
AGAGATTTCT ATGACAGTGC GGGAAAGCAG CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRCP ( NM_199418.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_955450.2 Homo sapiens prolylcarboxypeptidase (PRCP), transcript variant 2, mRNA.

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CloneID	OHu23893
Clone ID Related Accession (Same CDS sequence)	NM_199418.3
Accession Version	NM_199418.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	lysosomal Pro-X carboxypeptidase isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB444448.1, BI827978.1, AK091786.1, BC001500.2, AA477618.1, BE154586.1, CB154314.1, AP001646.5 and AW627417.1. On Jan 30, 2016 this sequence version replaced NM_199418.2. Transcript Variant: This variant (2) includes an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (2) is longer than isoform 1. This isoform (2) may undergo proteolytic processing similar to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK091786.1, SRR1803613.113558.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCCGCC GAGCCCTCCT GCTCCTGCTT CTGTCTTTTC TGGCGCCCTG GGCCACCATA 
GCCCTCCGGC CGGCCTTAAG GGCCCTCGGC AGCCTACACT TGCCAACCAA CCCCACATCC 
CTCCCGGCTG TAGCCAAGAA CTATTCGGTT CTCTACTTCC AACAGAAGGC CCTTGCTGCT 
GGGCAGTTGC ACATATGTAT TATCCAACTA AATCATTACA AGACTCCTCT GGTTGATCAT 
TTTGGATTTA ATACTGTGAA AACTTTTAAT CAGCGGTACC TAGTAGCTGA TAAATACTGG 
AAGAAAAATG GTGGATCAAT ACTTTTCTAC ACTGGTAATG AAGGGGACAT TATCTGGTTT 
TGTAATAACA CGGGGTTCAT GTGGGATGTG GCTGAGGAAC TGAAAGCTAT GTTGGTGTTT 
GCTGAACATC GATACTATGG AGAGTCTCTC CCCTTTGGTG ACAACTCATT CAAGGATTCC 
AGACACTTGA ATTTCCTGAC ATCAGAACAA GCTCTGGCTG ATTTTGCAGA GTTAATCAAA 
CACTTGAAAA GAACAATCCC AGGAGCTGAA AATCAACCTG TCATTGCCAT AGGAGGCTCC 
TATGGTGGCA TGCTTGCCGC CTGGTTTAGG ATGAAATATC CTCATATGGT AGTTGGAGCT 
CTTGCAGCTT CTGCCCCTAT CTGGCAGTTT GAGGATTTAG TACCTTGTGG TGTATTTATG 
AAGATCGTAA CTACAGATTT TAGGAAAAGC GGTCCACATT GTTCAGAGAG CATCCACAGG 
TCCTGGGATG CCATTAATCG ACTCTCAAAT ACTGGCAGTG GTTTGCAGTG GCTTACTGGA 
GCCCTTCACT TATGCAGCCC ATTAACTTCT CAGGACATCC AACATTTGAA AGACTGGATC 
TCTGAAACCT GGGTGAATCT GGCAATGGTG GACTATCCTT ATGCCTCTAA CTTTTTACAG 
CCTTTGCCTG CTTGGCCTAT CAAGGTAGTG TGCCAGTATT TGAAAAATCC CAATGTATCT 
GATTCACTGC TGCTGCAGAA TATTTTCCAA GCTCTGAATG TATATTACAA TTATTCGGGC 
CAGGTGAAAT GCCTGAATAT TTCAGAGACA GCAACTAGCA GTCTGGGAAC ACTGGGTTGG 
AGCTATCAGG CCTGCACAGA AGTAGTCATG CCCTTTTGTA CTAATGGTGT CGATGACATG 
TTTGAACCTC ACTCATGGAA CTTAAAGGAA CTTTCTGATG ACTGTTTTCA ACAGTGGGGT 
GTGAGACCAA GGCCCTCCTG GATCACTACT ATGTATGGAG GCAAAAACAT TAGTTCACAC 
ACAAACATTG TTTTCAGCAA TGGTGAACTA GACCCCTGGT CAGGAGGTGG AGTAACTAAG 
GATATCACAG ACACTCTGGT TGCAGTCACC ATCTCAGAGG GGGCCCACCA CTTAGATCTC 
CGCACCAAGA ATGCCTTGGA TCCTATGTCT GTGCTGTTAG CCCGCTCCTT GGAAGTTAGA 
CATATGAAGA ATTGGATCAG AGATTTCTAT GACAGTGCGG GAAAGCAGCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955450.2
CDS	202..1755
Translation	MGRRALLLLLLSFLAPWATIALRPALRALGSLHLPTNPTSLPAVAKNYSVLYFQQKALAAGQLHICIIQLNHYKTPLVDHFGFNTVKTFNQRYLVADKYWKKNGGSILFYTGNEGDIIWFCNNTGFMWDVAEELKAMLVFAEHRYYGESLPFGDNSFKDSRHLNFLTSEQALADFAELIKHLKRTIPGAENQPVIAIGGSYGGMLAAWFRMKYPHMVVGALAASAPIWQFEDLVPCGVFMKIVTTDFRKSGPHCSESIHRSWDAINRLSNTGSGLQWLTGALHLCSPLTSQDIQHLKDWISETWVNLAMVDYPYASNFLQPLPAWPIKVVCQYLKNPNVSDSLLLQNIFQALNVYYNYSGQVKCLNISETATSSLGTLGWSYQACTEVVMPFCTNGVDDMFEPHSWNLKELSDDCFQQWGVRPRPSWITTMYGGKNISSHTNIVFSNGELDPWSGGGVTKDITDTLVAVTISEGAHHLDLRTKNALDPMSVLLARSLEVRHMKNWIRDFYDSAGKQH

Target ORF information:

RefSeq Version	NM_199418.3
Organism	Homo sapiens(human)
Definition	Homo sapiens prolylcarboxypeptidase (PRCP), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199418.3

ORF Insert Sequence:

ATGGGCCGCC GAGCCCTCCT GCTCCTGCTT CTGTCTTTTC TGGCGCCCTG GGCCACCATA 
GCCCTCCGGC CGGCCTTAAG GGCCCTCGGC AGCCTACACT TGCCAACCAA CCCCACATCC 
CTCCCGGCTG TAGCCAAGAA CTATTCGGTT CTCTACTTCC AACAGAAGGC CCTTGCTGCT 
GGGCAGTTGC ACATATGTAT TATCCAACTA AATCATTACA AGACTCCTCT GGTTGATCAT 
TTTGGATTTA ATACTGTGAA AACTTTTAAT CAGCGGTACC TAGTAGCTGA TAAATACTGG 
AAGAAAAATG GTGGATCAAT ACTTTTCTAC ACTGGTAATG AAGGGGACAT TATCTGGTTT 
TGTAATAACA CGGGGTTCAT GTGGGATGTG GCTGAGGAAC TGAAAGCTAT GTTGGTGTTT 
GCTGAACATC GATACTATGG AGAGTCTCTC CCCTTTGGTG ACAACTCATT CAAGGATTCC 
AGACACTTGA ATTTCCTGAC ATCAGAACAA GCTCTGGCTG ATTTTGCAGA GTTAATCAAA 
CACTTGAAAA GAACAATCCC AGGAGCTGAA AATCAACCTG TCATTGCCAT AGGAGGCTCC 
TATGGTGGCA TGCTTGCCGC CTGGTTTAGG ATGAAATATC CTCATATGGT AGTTGGAGCT 
CTTGCAGCTT CTGCCCCTAT CTGGCAGTTT GAGGATTTAG TACCTTGTGG TGTATTTATG 
AAGATCGTAA CTACAGATTT TAGGAAAAGC GGTCCACATT GTTCAGAGAG CATCCACAGG 
TCCTGGGATG CCATTAATCG ACTCTCAAAT ACTGGCAGTG GTTTGCAGTG GCTTACTGGA 
GCCCTTCACT TATGCAGCCC ATTAACTTCT CAGGACATCC AACATTTGAA AGACTGGATC 
TCTGAAACCT GGGTGAATCT GGCAATGGTG GACTATCCTT ATGCCTCTAA CTTTTTACAG 
CCTTTGCCTG CTTGGCCTAT CAAGGTAGTG TGCCAGTATT TGAAAAATCC CAATGTATCT 
GATTCACTGC TGCTGCAGAA TATTTTCCAA GCTCTGAATG TATATTACAA TTATTCGGGC 
CAGGTGAAAT GCCTGAATAT TTCAGAGACA GCAACTAGCA GTCTGGGAAC ACTGGGTTGG 
AGCTATCAGG CCTGCACAGA AGTAGTCATG CCCTTTTGTA CTAATGGTGT CGATGACATG 
TTTGAACCTC ACTCATGGAA CTTAAAGGAA CTTTCTGATG ACTGTTTTCA ACAGTGGGGT 
GTGAGACCAA GGCCCTCCTG GATCACTACT ATGTATGGAG GCAAAAACAT TAGTTCACAC 
ACAAACATTG TTTTCAGCAA TGGTGAACTA GACCCCTGGT CAGGAGGTGG AGTAACTAAG 
GATATCACAG ACACTCTGGT TGCAGTCACC ATCTCAGAGG GGGCCCACCA CTTAGATCTC 
CGCACCAAGA ATGCCTTGGA TCCTATGTCT GTGCTGTTAG CCCGCTCCTT GGAAGTTAGA 
CATATGAAGA ATTGGATCAG AGATTTCTAT GACAGTGCGG GAAAGCAGCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of novel molecular signatures of IgA nephropathy through an integrative -omics analysis.
Scientific reports7(1)9091(2017 Aug)
Krochmal M,Cisek K,Filip S,Markoska K,Orange C,Zoidakis J,Gakiopoulou C,Spasovski G,Mischak H,Delles C,Vlahou A,Jankowski J

Prolyl carboxypeptidase purified from human placenta: its characterization and identification as an apelin-cleaving enzyme.
Biochimica et biophysica acta1864(11)1481-8(2016 11)
Kehoe K,Van Elzen R,Verkerk R,Sim Y,Van der Veken P,Lambeir AM,De Meester I

Prolyl carboxypeptidase activity decline correlates with severity and short-term outcome in acute ischemic stroke.
Neurochemical research40(1)81-8(2015 Jan)
Kehoe K,Brouns R,Verkerk R,Engelborghs S,De Deyn PP,Hendriks D,De Meester I

Prolylcarboxypeptidase independently activates plasma prekallikrein (fletcher factor).
Current molecular medicine14(9)1173-85(2014)
Wang J,Matafonov A,Madkhali H,Mahdi F,Watson D,Schmaier AH,Gailani D,Shariat-Madar Z

Prolylcarboxypeptidase promotes angiogenesis and vascular repair.
Blood122(8)1522-31(2013 Aug)
Adams GN,Stavrou EX,Fang C,Merkulova A,Alaiti MA,Nakajima K,Morooka T,Merkulov S,Larusch GA,Simon DI,Jain MK,Schmaier AH

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PRCP cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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