ZGRF1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017008369.1	XP_016863858.1	protein ZGRF1 isoform X3
XM_005263115.3	XP_005263172.1	protein ZGRF1 isoform X1
NM_001350397.1	NP_001337326.1	protein ZGRF1 isoform 2
XM_017008369.2	XP_016863858.1	protein ZGRF1 isoform X3
XM_011532094.2	XP_011530396.1	protein ZGRF1 isoform X5
XM_017008371.1	XP_016863860.1	protein ZGRF1 isoform X6
XM_011532097.2	XP_011530399.1	protein ZGRF1 isoform X8
XM_024454130.1	XP_024309898.1	protein ZGRF1 isoform X10
XM_005263115.4	XP_005263172.1	protein ZGRF1 isoform X1
XM_017008370.1	XP_016863859.1	protein ZGRF1 isoform X4
XM_011532099.2	XP_011530401.1	protein ZGRF1 isoform X9
NM_018392.4	NP_060862.3	protein ZGRF1 isoform 1
XM_011532092.2	XP_011530394.1	protein ZGRF1 isoform X3
XM_017008373.1	XP_016863862.1	protein ZGRF1 isoform X10
XM_011532091.2	XP_011530393.1	protein ZGRF1 isoform X2
XM_017008372.1	XP_016863861.1	protein ZGRF1 isoform X10
XM_024454129.1	XP_024309897.1	protein ZGRF1 isoform X10
XM_011532096.2	XP_011530398.1	protein ZGRF1 isoform X7
XM_011532098.2	XP_011530400.1	protein ZGRF1 isoform X9

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28391	NM_018392.4 Latest version!	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$779.50-$1091.30 $1559.00
OHu28391	XM_005263115.4 Latest version!	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$779.50-$1091.30 $1559.00
OHu95282	XM_024454129.1 Latest version!	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu95282	XM_017008372.1 Latest version!	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu95282	XM_024454130.1 Latest version!	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu95282	XM_017008373.1 Latest version!	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu69377	XM_011532097.2 Latest version!	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu69378	XM_011532099.2 Latest version!	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu69378	XM_011532098.2 Latest version!	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu69376	XM_011532096.2 Latest version!	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu95280	XM_017008370.1 Latest version!	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu95281	XM_017008371.1 Latest version!	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu69373	NM_001350397.1 Latest version!	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu69371	XM_011532091.2 Latest version!	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu69372	XM_017008369.2 Latest version!	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu69372	XM_011532092.2 Latest version!	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu69374	XM_011532094.2 Latest version!	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu69372	XM_017008369.1	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu28391	XM_005263115.3	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$779.50-$1091.30 $1559.00
Hide Old Accession Versions >>

ZGRF1 ( NM_018392.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060862.3 Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28391
Clone ID Related Accession (Same CDS sequence)	XM_005263115.3 , XM_005263115.4 , NM_018392.4
Accession Version	NM_018392.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	protein ZGRF1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB219969.1, AL708767.1, BC044799.1, AK299831.1, BM721145.1, DA199760.1, CN297341.1, AK126054.1 and BF056790.1. This sequence is a reference standard in the RefSeqGene project. On Jul 29, 2009 this sequence version replaced NM_018392.3. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAAAGCC AAGAATTTAT TGTTCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAATTTT ATATGATGAC 
AAAGGAGCAT GTTTGGAGAG TCTGTTTCTT AAATGCCTTG AGGTGAAACC TGGAGATGAC 
TTAGAAAGTG ATCGATACTT AATCACAGTT GAAGAGGTTA AAGTTGCTGG AGCCATAGGT 
ATTGTTAAGC AGAATGTCAA TAAAGAAGCA CCAGAGTTAA ATTCAAGGAC ATTTATATCC 
TCTGGCCGAT CTCTTGGATG TCAGCCCTCT GGCTTAAAAA GGAAGTTTAC TGGTTTTCAA 
GGACCACGTC AGGTTCCAAA GAAAATGGTT ATTATGGAAA GTGGTGAATC AGCTGCATCA 
CATGAGGCTA AGAAAACTGG CCCTACTATT TTTTCTCCAT TCTGCAGCAT GCCTCCTTTG 
TTTCCTACTG TTGGCAAGAA AGATGTAAAT AATATACTGG CAGACCCTGA GAACATTGTG 
ACTTACAAGA ACAGGGAGAG AAATGCCATG GATTTTTCTT CGGTTTTTTC TCCATCCTTC 
CAGATTAACC CAGAAGTGCT GTGTGAAGAA AATTATTTTT GCTCACCTGT CAATTCTGGA 
AATAAGCTTT CAGACTCTTT ACTGACCAAT GAGCCTGTGA AAAGAGATAG TTTGGCATCT 
CACTATTCAG GAGTTTCACA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTGAAG 
TCCGAATCAT CTAGTTCATG TGAGGAACTA AATTCTGAGA TGACAGAGCA TTTTCCTCAA 
AAACAACCAC AAGGAAGTTT AAAAATTGCT ACTAAACCAA AGTACCTAAT TCAACAGGAA 
GAGTGTGCTG AGATGAAGAG CACAGAAAAT TTATACTACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TATACATTCT 
TCTACTGTAG ATGGGAATGA TACAGAAAGG AAACCCAAGG CCCAGGAAGA TGATGTAAAT 
TCTAATTTGA AAGACCTTTC ATTACAAAAA ATTATACAGT TCGTTGAAAC GTATGCTGAA 
GAGAGGAAAA AGTATAATGT AGACCAGTCA GTCGGTAATA ATGATCCATC CTGGAATCAG 
GAAGTAAAAT TAGAAATTCC TTCATTCAAT GAAAGCAGTA GCTTACAGGT TACTTGTAGC 
AGTGCAGAAA ATGATGGTAT ATTATCAGAA TCTGACATTC AAGAAGATAA TAAAATACCT 
TTTAATCAAA ATGACAAGGG GTGCATTAAA GGATCAGTTC TCATTAAAGA AAATGCTCAG 
GAGGTAAATA CATGTGGAAC ACTGGAAAAG GAGTATGAAC AATCAGAGTC ATCTCTGCCA 
GAACTGAAAC ATCTCCAAAT TGAATCTAGT AATAATTCTA GGATCTCTGA TGACATTACA 
GACATGATTT CTGAAAGTAA AATGGATAAT GAAAGTCTTA ATAGTATTCA TGAATCTCTG 
AGTAATGTAA CACAACCATT TTTGGAGGTA ACTTTTAATC TGAACAATTT TGAGACCAGT 
GACACTGAGG AGGAATCACA GGAAAGCAAC AAAATTTCCC AGGATTCAGA GAGTTGGGTA 
AAGGACATTT TGGTTAATGA TGGCAATTCA TGTTTTCAAA AGAGGTCTGA GAATACAAAC 
TGTGAAGAGA TTGAGGGTGA ACATTTGCCA TTCTTAACAT CTGTTAGTGA CAAACCTACA 
GTGACATTTC CTGTTAAAGA GACTCTGCCA TCACAGTTTT GTGATAAAAC TTATGTGGGT 
TTTGACATGG GAATATGTAA AACTGAAAAC ACAGGAAAAG AAATAGAGGA GTATAGTGAC 
ACATTAAGCA ATTTTGAATC TTTCAAGTGG ACTGATGCTG TATACGGAGA TAATAAAGAA 
GATGCTAATA AACCTATTCA AGAAGTCAGA ATTAACTATG ATTTTGCTTT ACCCCCGAAT 
AAATCTAAAG GTATAAACAT GAATTTACAT ATTCCTCATA TTCAAAACCA GATTGCTGAA 
AACAGTAATC TATTTTCAGA AGATGCTCAA CCTCAGCCTT TTATTTTGGG AAGTGACTTA 
GACAAAAATG ATGAACATGT TTTACCCTCA ACTTCTAGTA GTGACAACAG TGTCCAACTA 
TTAAATACCA ATCAGAATCA CTATGAATGT ATTGCACTTG ATAAATCAAA TACCCACATT 
TCCAATTCTT TGTTTTACCC ACTGGGAAAA AAGCATCTTA TTTCCAAAGA CACAGAAGCA 
CATATATCTG AACCTGAAGA TTTGGGAAAG ATTAGAAGTC CACCCCCTGA CCATGTTGAG 
GTTGAAACTG CCAGAGAAGG CAAACAATAC TGGAATCCTA GAAATTCTTC AGAACTTTCT 
GGATTAGTAA ATACCATTTC TATTTTAAAG TCGCTATGTG AACACAGTAC TGCTTTAGAC 
AGCTTGGAAA TATTGAAAAA GAAAAATACT GTCTTTCAGC AAGGAACTCA ACAGACGTAT 
GAGCCAGATA GCCCTCCTGA AGTGAGGAAA CCATTTATTA CAGTAGTTTC ACCAAAGTCT 
CCTCATCTGC ACAAGGATTC TCAACAGATA CTAAAAGAAG ATGAAGTTGA ACTAAGTGAA 
CCACTTCAGT CTGTGCAGTT CTCTTCCTCG GGAAGTAAAG AAGAGACTGC TTTTCAAGCT 
GTTATTCCTA AACAAATAGA GAGAAAAACC TGTGACCCTA AGCCTGTTGA GTTTCAAGGA 
CATCAAGTAA AAGGATCAGC TACTAGTGGT GTAATGGTCA GAGGACACAG CTCACAGCTA 
GGATGCAGTC AGTTTCCAGA TAGCACTGAG TATGAAAACT TTATGACAGA GACACCAGAG 
TTACCTAGCA CGTGTATGCA GATTGACTTC TTGCAGGTGA CATCACCAGA AGAAAACATC 
TCTACATTGA GCCCTGTTTC TACCTTTTCT TTGAACTCAA GAGATGAAGA CTTCATGGTA 
GAATTCTCTG AGACGTCCCT GAAAGCAAGA ACTTTACCTG ATGATCTTCA TTTTCTCAAC 
TTGGAGGGGA TGAAAAAATC CCGTTCTCTG GAGAATGAGA ACCTTCAAAG GCTTTCATTA 
TTAAGTAGAA CCCAGGTTCC ACTTATTACT TTGCCACGTA CTGATGGGCC ACCTGACTTA 
GACTCTCATT CGTATATGAT CAACTCTAAC ACATACGAGT CTTCTGGCTC CCCCATGCTC 
AATTTGTGTG AAAAGTCAGC AGTTCTTTCG TTTAGCATTG AGCCTGAGGA CCAAAATGAA 
ACCTTTTTCT CTGAAGAATC TAGGGAAGTG AATCCAGGGG ATGTTTCACT TAATAATATA 
TCTACTCAGA GCAAGTGGCT GAAATATCAA AACACATCCC AATGCAACGT GGCTACTCCA 
AACAGAGTTG ATAAGAGAAT AACTGATGGC TTCTTTGCTG AGGCTGTTTC TGGGATGCAT 
TTTAGAGACA CAAGTGAAAG ACAGAGTGAT GCTGTCAATG AAAGCTCTTT AGACTCTGTG 
CATTTGCAAA TGATAAAAGG CATGCTCTAT CAACAGCGGC AGGATTTTAG CAGTCAAGAT 
TCGGTTTCCA GAAAGAAAGT ACTTTCTCTG AATTTAAAGC AGACTTCTAA GACAGAGGAA 
ATTAAAAATG TATTAGGAGG GTCTACCTGC TACAACTACA GTGTAAAGGA TTTACAGGAG 
ATAAGTGGCT CTGAGCTGTG CTTTCCAAGT GGGCAGAAAA TAAAATCTGC TTATCTTCCC 
CAAAGGCAAA TTCACATACC AGCTGTTTTT CAGTCTCCTG CTCATTATAA GCAGACTTTC 
ACATCTTGCC TCATAGAACA TCTAAATATA TTGCTGTTTG GGTTAGCACA AAACCTGCAG 
AAAGCTCTTT CAAAAGTTGA CATATCATTT TATACATCAT TGAAGGGAGA GAAACTGAAA 
AACGCAGAAA ATAATGTACC ATCCTGCCAT CATAGTCAAC CTGCAAAACT TGTCATGGTT 
AAAAAGGAAG GTCCAAATAA GGGTCGTCTC TTTTATACAT GTGATGGACC CAAAGCTGAT 
CGATGTAAAT TCTTTAAATG GCTTGAGGAC GTGACTCCAG GATATTCAAC ACAGGAAGGA 
GCTCGACCTG GCATGGTTTT AAGTGATATT AAGAGTATTG GCTTATATTT AAGAAGTCAA 
AAGATACCAC TTTATGAGGA ATGCCAGCTT TTGGTGAGAA AAGGATTTGA TTTTCAGAGA 
AAACAGTATG GCAAACTAAA GAAGTTTACT ACTGTAAATC CTGAGTTTTA TAATGAACCA 
AAAACCAAAC TTTATCTTAA GCTAAGTCGG AAGGAAAGAT CTTCAGCTTA TAGCAAAAAT 
GATCTTTGGG TGGTTTCAAA AACCCTAGAC TTTGAGCTGG ATACTTTTAT CGCATGTAGT 
GCTTTCTTTG GACCATCATC TATCAATGAG ATAGAAATAC TGCCTTTGAA AGGCTATTTC 
CCTTCTAATT GGCCCACTAA CATGGTTGTC CATGCGTTAT TGGTTTGTAA TGCTAGCACA 
GAACTGACTA CTTTGAAAAA CATTCAGGAC TACTTTAATC CAGCTACTCT ACCTCTAACA 
CAGTACCTGT TAACAACGTC TTCGCCAACT ATAGTTAGTA ACAAAAGAGT CAGTAAGAGA 
AAATTTATCC CACCAGCCTT CACAAATGTC AGTACAAAAT TTGAACTACT CAGCCTAGGA 
GCAACATTGA AGTTAGCTAG TGAGTTGATT CAGGTACACA AGTTAAACAA GGATCAAGCT 
ACAGCTCTAA TTCAAATAGC TCAAATGATG GCATCACATG AAAGCATTGA AGAAGTGAAG 
GAACTGCAAA CTCATACCTT CCCTATCACA ATCATACATG GTGTGTTTGG AGCAGGAAAG 
AGTTACTTGC TGGCAGTGGT GATTTTGTTC TTTGTACAGC TGTTTGAAAA GAGTGAAGCT 
CCCACCATTG GAAATGCAAG GCCGTGGAAA CTTCTGATTT CTTCTTCTAC TAATGTGGCT 
GTTGACAGAG TACTTCTTGG GCTTCTCAGT CTTGGATTTG AAAACTTTAT CAGAGTTGGG 
AGTGTTAGGA AGATTGCCAA ACCAATTTTA CCTTATAGCT TGCATGCTGG CTCAGAAAAT 
GAAAGTGAAC AGTTAAAAGA ACTACATGCA CTAATGAAAG AAGACCTGAC TCCTACGGAA 
AGAGTCTATG TGAGAAAAAG CATTGAGCAG CATAAACTGG GGACCAATAG AACCCTGCTG 
AAGCAGGTTC GAGTAGTTGG AGTTACCTGT GCAGCCTGCC CATTCCCATG CATGAATGAT 
CTTAAATTTC CTGTAGTTGT GCTGGATGAG TGTAGTCAGA TAACTGAACC GGCCTCTCTC 
CTTCCCATTG CAAGGTTTGA GTGTGAAAAG CTGATTCTTG TTGGGGATCC CAAACAGCTA 
CCTCCTACTA TTCAGGGTTC TGATGCAGCT CATGAAAATG GATTGGAACA AACTCTTTTT 
GATCGACTTT GCTTAATGGG TCACAAGCCA ATTCTATTGA GAACTCAATA CCGTTGTCAT 
CCTGCAATCA GTGCTATTGC TAATGATCTG TTTTACAAAG GAGCCCTCAT GAATGGTGTA 
ACAGAAATAG AGCGGAGCCC TTTATTGGAA TGGCTACCAA CCCTGTGTTT TTATAATGTT 
AAAGGACTAG AACAGATAGA AAGAGATAAC AGCTTTCATA ATGTGGCAGA AGCTACGTTT 
ACACTCAAGC TGATTCAATC ACTGATTGCA AGTGGAATAG CAGGCTCTAT GATTGGTGTG 
ATAACATTAT ACAAATCCCA GATGTACAAG CTTTGTCATT TACTCAGTGC TGTGGACTTT 
CACCATCCTG ATATTAAAAC TGTGCAGGTG TCCACAGTAG ATGCTTTTCA GGGAGCTGAA 
AAGGAGATCA TTATTCTGTC CTGTGTAAGG ACAAGACAAG TAGGATTCAT TGATTCAGAA 
AAAAGAATGA ATGTTGCATT GACTAGAGGA AAGAGGCATT TGTTGATTGT GGGAAATTTA 
GCCTGTTTGA GGAAAAATCA ACTTTGGGGA CGAGTGATCC AACACTGCGA AGGAAGGGAA 
GATGGATTGC AACATGCAAA CCAGTATGAA CCACAGCTGA ACCATCTCCT TAAAGATTAT 
TTTGAAAAAC AAGTGGAAGA AAAACAGAAG AAAAAGAGTG AAAAAGAGAA ATCTAAAGAT 
AAATCTCATT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060862.3
CDS	212..6526
Translation	MESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGACLESLFLKCLEVKPGDDLESDRYLITVEEVKVAGAIGIVKQNVNKEAPELNSRTFISSGRSLGCQPSGLKRKFTGFQGPRQVPKKMVIMESGESAASHEAKKTGPTIFSPFCSMPPLFPTVGKKDVNNILADPENIVTYKNRERNAMDFSSVFSPSFQINPEVLCEENYFCSPVNSGNKLSDSLLTNEPVKRDSLASHYSGVSQNIRSKAQILALLKSESSSSCEELNSEMTEHFPQKQPQGSLKIATKPKYLIQQEECAEMKSTENLYYQHQSENTMRNKSRWAMYLSSQSSPIHSSTVDGNDTERKPKAQEDDVNSNLKDLSLQKIIQFVETYAEERKKYNVDQSVGNNDPSWNQEVKLEIPSFNESSSLQVTCSSAENDGILSESDIQEDNKIPFNQNDKGCIKGSVLIKENAQEVNTCGTLEKEYEQSESSLPELKHLQIESSNNSRISDDITDMISESKMDNESLNSIHESLSNVTQPFLEVTFNLNNFETSDTEEESQESNKISQDSESWVKDILVNDGNSCFQKRSENTNCEEIEGEHLPFLTSVSDKPTVTFPVKETLPSQFCDKTYVGFDMGICKTENTGKEIEEYSDTLSNFESFKWTDAVYGDNKEDANKPIQEVRINYDFALPPNKSKGINMNLHIPHIQNQIAENSNLFSEDAQPQPFILGSDLDKNDEHVLPSTSSSDNSVQLLNTNQNHYECIALDKSNTHISNSLFYPLGKKHLISKDTEAHISEPEDLGKIRSPPPDHVEVETAREGKQYWNPRNSSELSGLVNTISILKSLCEHSTALDSLEILKKKNTVFQQGTQQTYEPDSPPEVRKPFITVVSPKSPHLHKDSQQILKEDEVELSEPLQSVQFSSSGSKEETAFQAVIPKQIERKTCDPKPVEFQGHQVKGSATSGVMVRGHSSQLGCSQFPDSTEYENFMTETPELPSTCMQIDFLQVTSPEENISTLSPVSTFSLNSRDEDFMVEFSETSLKARTLPDDLHFLNLEGMKKSRSLENENLQRLSLLSRTQVPLITLPRTDGPPDLDSHSYMINSNTYESSGSPMLNLCEKSAVLSFSIEPEDQNETFFSEESREVNPGDVSLNNISTQSKWLKYQNTSQCNVATPNRVDKRITDGFFAEAVSGMHFRDTSERQSDAVNESSLDSVHLQMIKGMLYQQRQDFSSQDSVSRKKVLSLNLKQTSKTEEIKNVLGGSTCYNYSVKDLQEISGSELCFPSGQKIKSAYLPQRQIHIPAVFQSPAHYKQTFTSCLIEHLNILLFGLAQNLQKALSKVDISFYTSLKGEKLKNAENNVPSCHHSQPAKLVMVKKEGPNKGRLFYTCDGPKADRCKFFKWLEDVTPGYSTQEGARPGMVLSDIKSIGLYLRSQKIPLYEECQLLVRKGFDFQRKQYGKLKKFTTVNPEFYNEPKTKLYLKLSRKERSSAYSKNDLWVVSKTLDFELDTFIACSAFFGPSSINEIEILPLKGYFPSNWPTNMVVHALLVCNASTELTTLKNIQDYFNPATLPLTQYLLTTSSPTIVSNKRVSKRKFIPPAFTNVSTKFELLSLGATLKLASELIQVHKLNKDQATALIQIAQMMASHESIEEVKELQTHTFPITIIHGVFGAGKSYLLAVVILFFVQLFEKSEAPTIGNARPWKLLISSSTNVAVDRVLLGLLSLGFENFIRVGSVRKIAKPILPYSLHAGSENESEQLKELHALMKEDLTPTERVYVRKSIEQHKLGTNRTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPAISAIANDLFYKGALMNGVTEIERSPLLEWLPTLCFYNVKGLEQIERDNSFHNVAEATFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAVDFHHPDIKTVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGKRHLLIVGNLACLRKNQLWGRVIQHCEGREDGLQHANQYEPQLNHLLKDYFEKQVEEKQKKKSEKEKSKDKSHS

Target ORF information:

RefSeq Version	NM_018392.4
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018392.4

ORF Insert Sequence:

ATGGAAAGCC AAGAATTTAT TGTTCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAATTTT ATATGATGAC 
AAAGGAGCAT GTTTGGAGAG TCTGTTTCTT AAATGCCTTG AGGTGAAACC TGGAGATGAC 
TTAGAAAGTG ATCGATACTT AATCACAGTT GAAGAGGTTA AAGTTGCTGG AGCCATAGGT 
ATTGTTAAGC AGAATGTCAA TAAAGAAGCA CCAGAGTTAA ATTCAAGGAC ATTTATATCC 
TCTGGCCGAT CTCTTGGATG TCAGCCCTCT GGCTTAAAAA GGAAGTTTAC TGGTTTTCAA 
GGACCACGTC AGGTTCCAAA GAAAATGGTT ATTATGGAAA GTGGTGAATC AGCTGCATCA 
CATGAGGCTA AGAAAACTGG CCCTACTATT TTTTCTCCAT TCTGCAGCAT GCCTCCTTTG 
TTTCCTACTG TTGGCAAGAA AGATGTAAAT AATATACTGG CAGACCCTGA GAACATTGTG 
ACTTACAAGA ACAGGGAGAG AAATGCCATG GATTTTTCTT CGGTTTTTTC TCCATCCTTC 
CAGATTAACC CAGAAGTGCT GTGTGAAGAA AATTATTTTT GCTCACCTGT CAATTCTGGA 
AATAAGCTTT CAGACTCTTT ACTGACCAAT GAGCCTGTGA AAAGAGATAG TTTGGCATCT 
CACTATTCAG GAGTTTCACA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTGAAG 
TCCGAATCAT CTAGTTCATG TGAGGAACTA AATTCTGAGA TGACAGAGCA TTTTCCTCAA 
AAACAACCAC AAGGAAGTTT AAAAATTGCT ACTAAACCAA AGTACCTAAT TCAACAGGAA 
GAGTGTGCTG AGATGAAGAG CACAGAAAAT TTATACTACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TATACATTCT 
TCTACTGTAG ATGGGAATGA TACAGAAAGG AAACCCAAGG CCCAGGAAGA TGATGTAAAT 
TCTAATTTGA AAGACCTTTC ATTACAAAAA ATTATACAGT TCGTTGAAAC GTATGCTGAA 
GAGAGGAAAA AGTATAATGT AGACCAGTCA GTCGGTAATA ATGATCCATC CTGGAATCAG 
GAAGTAAAAT TAGAAATTCC TTCATTCAAT GAAAGCAGTA GCTTACAGGT TACTTGTAGC 
AGTGCAGAAA ATGATGGTAT ATTATCAGAA TCTGACATTC AAGAAGATAA TAAAATACCT 
TTTAATCAAA ATGACAAGGG GTGCATTAAA GGATCAGTTC TCATTAAAGA AAATGCTCAG 
GAGGTAAATA CATGTGGAAC ACTGGAAAAG GAGTATGAAC AATCAGAGTC ATCTCTGCCA 
GAACTGAAAC ATCTCCAAAT TGAATCTAGT AATAATTCTA GGATCTCTGA TGACATTACA 
GACATGATTT CTGAAAGTAA AATGGATAAT GAAAGTCTTA ATAGTATTCA TGAATCTCTG 
AGTAATGTAA CACAACCATT TTTGGAGGTA ACTTTTAATC TGAACAATTT TGAGACCAGT 
GACACTGAGG AGGAATCACA GGAAAGCAAC AAAATTTCCC AGGATTCAGA GAGTTGGGTA 
AAGGACATTT TGGTTAATGA TGGCAATTCA TGTTTTCAAA AGAGGTCTGA GAATACAAAC 
TGTGAAGAGA TTGAGGGTGA ACATTTGCCA TTCTTAACAT CTGTTAGTGA CAAACCTACA 
GTGACATTTC CTGTTAAAGA GACTCTGCCA TCACAGTTTT GTGATAAAAC TTATGTGGGT 
TTTGACATGG GAATATGTAA AACTGAAAAC ACAGGAAAAG AAATAGAGGA GTATAGTGAC 
ACATTAAGCA ATTTTGAATC TTTCAAGTGG ACTGATGCTG TATACGGAGA TAATAAAGAA 
GATGCTAATA AACCTATTCA AGAAGTCAGA ATTAACTATG ATTTTGCTTT ACCCCCGAAT 
AAATCTAAAG GTATAAACAT GAATTTACAT ATTCCTCATA TTCAAAACCA GATTGCTGAA 
AACAGTAATC TATTTTCAGA AGATGCTCAA CCTCAGCCTT TTATTTTGGG AAGTGACTTA 
GACAAAAATG ATGAACATGT TTTACCCTCA ACTTCTAGTA GTGACAACAG TGTCCAACTA 
TTAAATACCA ATCAGAATCA CTATGAATGT ATTGCACTTG ATAAATCAAA TACCCACATT 
TCCAATTCTT TGTTTTACCC ACTGGGAAAA AAGCATCTTA TTTCCAAAGA CACAGAAGCA 
CATATATCTG AACCTGAAGA TTTGGGAAAG ATTAGAAGTC CACCCCCTGA CCATGTTGAG 
GTTGAAACTG CCAGAGAAGG CAAACAATAC TGGAATCCTA GAAATTCTTC AGAACTTTCT 
GGATTAGTAA ATACCATTTC TATTTTAAAG TCGCTATGTG AACACAGTAC TGCTTTAGAC 
AGCTTGGAAA TATTGAAAAA GAAAAATACT GTCTTTCAGC AAGGAACTCA ACAGACGTAT 
GAGCCAGATA GCCCTCCTGA AGTGAGGAAA CCATTTATTA CAGTAGTTTC ACCAAAGTCT 
CCTCATCTGC ACAAGGATTC TCAACAGATA CTAAAAGAAG ATGAAGTTGA ACTAAGTGAA 
CCACTTCAGT CTGTGCAGTT CTCTTCCTCG GGAAGTAAAG AAGAGACTGC TTTTCAAGCT 
GTTATTCCTA AACAAATAGA GAGAAAAACC TGTGACCCTA AGCCTGTTGA GTTTCAAGGA 
CATCAAGTAA AAGGATCAGC TACTAGTGGT GTAATGGTCA GAGGACACAG CTCACAGCTA 
GGATGCAGTC AGTTTCCAGA TAGCACTGAG TATGAAAACT TTATGACAGA GACACCAGAG 
TTACCTAGCA CGTGTATGCA GATTGACTTC TTGCAGGTGA CATCACCAGA AGAAAACATC 
TCTACATTGA GCCCTGTTTC TACCTTTTCT TTGAACTCAA GAGATGAAGA CTTCATGGTA 
GAATTCTCTG AGACGTCCCT GAAAGCAAGA ACTTTACCTG ATGATCTTCA TTTTCTCAAC 
TTGGAGGGGA TGAAAAAATC CCGTTCTCTG GAGAATGAGA ACCTTCAAAG GCTTTCATTA 
TTAAGTAGAA CCCAGGTTCC ACTTATTACT TTGCCACGTA CTGATGGGCC ACCTGACTTA 
GACTCTCATT CGTATATGAT CAACTCTAAC ACATACGAGT CTTCTGGCTC CCCCATGCTC 
AATTTGTGTG AAAAGTCAGC AGTTCTTTCG TTTAGCATTG AGCCTGAGGA CCAAAATGAA 
ACCTTTTTCT CTGAAGAATC TAGGGAAGTG AATCCAGGGG ATGTTTCACT TAATAATATA 
TCTACTCAGA GCAAGTGGCT GAAATATCAA AACACATCCC AATGCAACGT GGCTACTCCA 
AACAGAGTTG ATAAGAGAAT AACTGATGGC TTCTTTGCTG AGGCTGTTTC TGGGATGCAT 
TTTAGAGACA CAAGTGAAAG ACAGAGTGAT GCTGTCAATG AAAGCTCTTT AGACTCTGTG 
CATTTGCAAA TGATAAAAGG CATGCTCTAT CAACAGCGGC AGGATTTTAG CAGTCAAGAT 
TCGGTTTCCA GAAAGAAAGT ACTTTCTCTG AATTTAAAGC AGACTTCTAA GACAGAGGAA 
ATTAAAAATG TATTAGGAGG GTCTACCTGC TACAACTACA GTGTAAAGGA TTTACAGGAG 
ATAAGTGGCT CTGAGCTGTG CTTTCCAAGT GGGCAGAAAA TAAAATCTGC TTATCTTCCC 
CAAAGGCAAA TTCACATACC AGCTGTTTTT CAGTCTCCTG CTCATTATAA GCAGACTTTC 
ACATCTTGCC TCATAGAACA TCTAAATATA TTGCTGTTTG GGTTAGCACA AAACCTGCAG 
AAAGCTCTTT CAAAAGTTGA CATATCATTT TATACATCAT TGAAGGGAGA GAAACTGAAA 
AACGCAGAAA ATAATGTACC ATCCTGCCAT CATAGTCAAC CTGCAAAACT TGTCATGGTT 
AAAAAGGAAG GTCCAAATAA GGGTCGTCTC TTTTATACAT GTGATGGACC CAAAGCTGAT 
CGATGTAAAT TCTTTAAATG GCTTGAGGAC GTGACTCCAG GATATTCAAC ACAGGAAGGA 
GCTCGACCTG GCATGGTTTT AAGTGATATT AAGAGTATTG GCTTATATTT AAGAAGTCAA 
AAGATACCAC TTTATGAGGA ATGCCAGCTT TTGGTGAGAA AAGGATTTGA TTTTCAGAGA 
AAACAGTATG GCAAACTAAA GAAGTTTACT ACTGTAAATC CTGAGTTTTA TAATGAACCA 
AAAACCAAAC TTTATCTTAA GCTAAGTCGG AAGGAAAGAT CTTCAGCTTA TAGCAAAAAT 
GATCTTTGGG TGGTTTCAAA AACCCTAGAC TTTGAGCTGG ATACTTTTAT CGCATGTAGT 
GCTTTCTTTG GACCATCATC TATCAATGAG ATAGAAATAC TGCCTTTGAA AGGCTATTTC 
CCTTCTAATT GGCCCACTAA CATGGTTGTC CATGCGTTAT TGGTTTGTAA TGCTAGCACA 
GAACTGACTA CTTTGAAAAA CATTCAGGAC TACTTTAATC CAGCTACTCT ACCTCTAACA 
CAGTACCTGT TAACAACGTC TTCGCCAACT ATAGTTAGTA ACAAAAGAGT CAGTAAGAGA 
AAATTTATCC CACCAGCCTT CACAAATGTC AGTACAAAAT TTGAACTACT CAGCCTAGGA 
GCAACATTGA AGTTAGCTAG TGAGTTGATT CAGGTACACA AGTTAAACAA GGATCAAGCT 
ACAGCTCTAA TTCAAATAGC TCAAATGATG GCATCACATG AAAGCATTGA AGAAGTGAAG 
GAACTGCAAA CTCATACCTT CCCTATCACA ATCATACATG GTGTGTTTGG AGCAGGAAAG 
AGTTACTTGC TGGCAGTGGT GATTTTGTTC TTTGTACAGC TGTTTGAAAA GAGTGAAGCT 
CCCACCATTG GAAATGCAAG GCCGTGGAAA CTTCTGATTT CTTCTTCTAC TAATGTGGCT 
GTTGACAGAG TACTTCTTGG GCTTCTCAGT CTTGGATTTG AAAACTTTAT CAGAGTTGGG 
AGTGTTAGGA AGATTGCCAA ACCAATTTTA CCTTATAGCT TGCATGCTGG CTCAGAAAAT 
GAAAGTGAAC AGTTAAAAGA ACTACATGCA CTAATGAAAG AAGACCTGAC TCCTACGGAA 
AGAGTCTATG TGAGAAAAAG CATTGAGCAG CATAAACTGG GGACCAATAG AACCCTGCTG 
AAGCAGGTTC GAGTAGTTGG AGTTACCTGT GCAGCCTGCC CATTCCCATG CATGAATGAT 
CTTAAATTTC CTGTAGTTGT GCTGGATGAG TGTAGTCAGA TAACTGAACC GGCCTCTCTC 
CTTCCCATTG CAAGGTTTGA GTGTGAAAAG CTGATTCTTG TTGGGGATCC CAAACAGCTA 
CCTCCTACTA TTCAGGGTTC TGATGCAGCT CATGAAAATG GATTGGAACA AACTCTTTTT 
GATCGACTTT GCTTAATGGG TCACAAGCCA ATTCTATTGA GAACTCAATA CCGTTGTCAT 
CCTGCAATCA GTGCTATTGC TAATGATCTG TTTTACAAAG GAGCCCTCAT GAATGGTGTA 
ACAGAAATAG AGCGGAGCCC TTTATTGGAA TGGCTACCAA CCCTGTGTTT TTATAATGTT 
AAAGGACTAG AACAGATAGA AAGAGATAAC AGCTTTCATA ATGTGGCAGA AGCTACGTTT 
ACACTCAAGC TGATTCAATC ACTGATTGCA AGTGGAATAG CAGGCTCTAT GATTGGTGTG 
ATAACATTAT ACAAATCCCA GATGTACAAG CTTTGTCATT TACTCAGTGC TGTGGACTTT 
CACCATCCTG ATATTAAAAC TGTGCAGGTG TCCACAGTAG ATGCTTTTCA GGGAGCTGAA 
AAGGAGATCA TTATTCTGTC CTGTGTAAGG ACAAGACAAG TAGGATTCAT TGATTCAGAA 
AAAAGAATGA ATGTTGCATT GACTAGAGGA AAGAGGCATT TGTTGATTGT GGGAAATTTA 
GCCTGTTTGA GGAAAAATCA ACTTTGGGGA CGAGTGATCC AACACTGCGA AGGAAGGGAA 
GATGGATTGC AACATGCAAA CCAGTATGAA CCACAGCTGA ACCATCTCCT TAAAGATTAT 
TTTGAAAAAC AAGTGGAAGA AAAACAGAAG AAAAAGAGTG AAAAAGAGAA ATCTAAAGAT 
AAATCTCATT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_005263115.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263172.1 Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X1, mRNA.

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CloneID	OHu28391
Clone ID Related Accession (Same CDS sequence)	XM_005263115.3 , XM_005263115.4 , NM_018392.4
Accession Version	XM_005263115.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein ZGRF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263115.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGCC AAGAATTTAT TGTTCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAATTTT ATATGATGAC 
AAAGGAGCAT GTTTGGAGAG TCTGTTTCTT AAATGCCTTG AGGTGAAACC TGGAGATGAC 
TTAGAAAGTG ATCGATACTT AATCACAGTT GAAGAGGTTA AAGTTGCTGG AGCCATAGGT 
ATTGTTAAGC AGAATGTCAA TAAAGAAGCA CCAGAGTTAA ATTCAAGGAC ATTTATATCC 
TCTGGCCGAT CTCTTGGATG TCAGCCCTCT GGCTTAAAAA GGAAGTTTAC TGGTTTTCAA 
GGACCACGTC AGGTTCCAAA GAAAATGGTT ATTATGGAAA GTGGTGAATC AGCTGCATCA 
CATGAGGCTA AGAAAACTGG CCCTACTATT TTTTCTCCAT TCTGCAGCAT GCCTCCTTTG 
TTTCCTACTG TTGGCAAGAA AGATGTAAAT AATATACTGG CAGACCCTGA GAACATTGTG 
ACTTACAAGA ACAGGGAGAG AAATGCCATG GATTTTTCTT CGGTTTTTTC TCCATCCTTC 
CAGATTAACC CAGAAGTGCT GTGTGAAGAA AATTATTTTT GCTCACCTGT CAATTCTGGA 
AATAAGCTTT CAGACTCTTT ACTGACCAAT GAGCCTGTGA AAAGAGATAG TTTGGCATCT 
CACTATTCAG GAGTTTCACA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTGAAG 
TCCGAATCAT CTAGTTCATG TGAGGAACTA AATTCTGAGA TGACAGAGCA TTTTCCTCAA 
AAACAACCAC AAGGAAGTTT AAAAATTGCT ACTAAACCAA AGTACCTAAT TCAACAGGAA 
GAGTGTGCTG AGATGAAGAG CACAGAAAAT TTATACTACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TATACATTCT 
TCTACTGTAG ATGGGAATGA TACAGAAAGG AAACCCAAGG CCCAGGAAGA TGATGTAAAT 
TCTAATTTGA AAGACCTTTC ATTACAAAAA ATTATACAGT TCGTTGAAAC GTATGCTGAA 
GAGAGGAAAA AGTATAATGT AGACCAGTCA GTCGGTAATA ATGATCCATC CTGGAATCAG 
GAAGTAAAAT TAGAAATTCC TTCATTCAAT GAAAGCAGTA GCTTACAGGT TACTTGTAGC 
AGTGCAGAAA ATGATGGTAT ATTATCAGAA TCTGACATTC AAGAAGATAA TAAAATACCT 
TTTAATCAAA ATGACAAGGG GTGCATTAAA GGATCAGTTC TCATTAAAGA AAATGCTCAG 
GAGGTAAATA CATGTGGAAC ACTGGAAAAG GAGTATGAAC AATCAGAGTC ATCTCTGCCA 
GAACTGAAAC ATCTCCAAAT TGAATCTAGT AATAATTCTA GGATCTCTGA TGACATTACA 
GACATGATTT CTGAAAGTAA AATGGATAAT GAAAGTCTTA ATAGTATTCA TGAATCTCTG 
AGTAATGTAA CACAACCATT TTTGGAGGTA ACTTTTAATC TGAACAATTT TGAGACCAGT 
GACACTGAGG AGGAATCACA GGAAAGCAAC AAAATTTCCC AGGATTCAGA GAGTTGGGTA 
AAGGACATTT TGGTTAATGA TGGCAATTCA TGTTTTCAAA AGAGGTCTGA GAATACAAAC 
TGTGAAGAGA TTGAGGGTGA ACATTTGCCA TTCTTAACAT CTGTTAGTGA CAAACCTACA 
GTGACATTTC CTGTTAAAGA GACTCTGCCA TCACAGTTTT GTGATAAAAC TTATGTGGGT 
TTTGACATGG GAATATGTAA AACTGAAAAC ACAGGAAAAG AAATAGAGGA GTATAGTGAC 
ACATTAAGCA ATTTTGAATC TTTCAAGTGG ACTGATGCTG TATACGGAGA TAATAAAGAA 
GATGCTAATA AACCTATTCA AGAAGTCAGA ATTAACTATG ATTTTGCTTT ACCCCCGAAT 
AAATCTAAAG GTATAAACAT GAATTTACAT ATTCCTCATA TTCAAAACCA GATTGCTGAA 
AACAGTAATC TATTTTCAGA AGATGCTCAA CCTCAGCCTT TTATTTTGGG AAGTGACTTA 
GACAAAAATG ATGAACATGT TTTACCCTCA ACTTCTAGTA GTGACAACAG TGTCCAACTA 
TTAAATACCA ATCAGAATCA CTATGAATGT ATTGCACTTG ATAAATCAAA TACCCACATT 
TCCAATTCTT TGTTTTACCC ACTGGGAAAA AAGCATCTTA TTTCCAAAGA CACAGAAGCA 
CATATATCTG AACCTGAAGA TTTGGGAAAG ATTAGAAGTC CACCCCCTGA CCATGTTGAG 
GTTGAAACTG CCAGAGAAGG CAAACAATAC TGGAATCCTA GAAATTCTTC AGAACTTTCT 
GGATTAGTAA ATACCATTTC TATTTTAAAG TCGCTATGTG AACACAGTAC TGCTTTAGAC 
AGCTTGGAAA TATTGAAAAA GAAAAATACT GTCTTTCAGC AAGGAACTCA ACAGACGTAT 
GAGCCAGATA GCCCTCCTGA AGTGAGGAAA CCATTTATTA CAGTAGTTTC ACCAAAGTCT 
CCTCATCTGC ACAAGGATTC TCAACAGATA CTAAAAGAAG ATGAAGTTGA ACTAAGTGAA 
CCACTTCAGT CTGTGCAGTT CTCTTCCTCG GGAAGTAAAG AAGAGACTGC TTTTCAAGCT 
GTTATTCCTA AACAAATAGA GAGAAAAACC TGTGACCCTA AGCCTGTTGA GTTTCAAGGA 
CATCAAGTAA AAGGATCAGC TACTAGTGGT GTAATGGTCA GAGGACACAG CTCACAGCTA 
GGATGCAGTC AGTTTCCAGA TAGCACTGAG TATGAAAACT TTATGACAGA GACACCAGAG 
TTACCTAGCA CGTGTATGCA GATTGACTTC TTGCAGGTGA CATCACCAGA AGAAAACATC 
TCTACATTGA GCCCTGTTTC TACCTTTTCT TTGAACTCAA GAGATGAAGA CTTCATGGTA 
GAATTCTCTG AGACGTCCCT GAAAGCAAGA ACTTTACCTG ATGATCTTCA TTTTCTCAAC 
TTGGAGGGGA TGAAAAAATC CCGTTCTCTG GAGAATGAGA ACCTTCAAAG GCTTTCATTA 
TTAAGTAGAA CCCAGGTTCC ACTTATTACT TTGCCACGTA CTGATGGGCC ACCTGACTTA 
GACTCTCATT CGTATATGAT CAACTCTAAC ACATACGAGT CTTCTGGCTC CCCCATGCTC 
AATTTGTGTG AAAAGTCAGC AGTTCTTTCG TTTAGCATTG AGCCTGAGGA CCAAAATGAA 
ACCTTTTTCT CTGAAGAATC TAGGGAAGTG AATCCAGGGG ATGTTTCACT TAATAATATA 
TCTACTCAGA GCAAGTGGCT GAAATATCAA AACACATCCC AATGCAACGT GGCTACTCCA 
AACAGAGTTG ATAAGAGAAT AACTGATGGC TTCTTTGCTG AGGCTGTTTC TGGGATGCAT 
TTTAGAGACA CAAGTGAAAG ACAGAGTGAT GCTGTCAATG AAAGCTCTTT AGACTCTGTG 
CATTTGCAAA TGATAAAAGG CATGCTCTAT CAACAGCGGC AGGATTTTAG CAGTCAAGAT 
TCGGTTTCCA GAAAGAAAGT ACTTTCTCTG AATTTAAAGC AGACTTCTAA GACAGAGGAA 
ATTAAAAATG TATTAGGAGG GTCTACCTGC TACAACTACA GTGTAAAGGA TTTACAGGAG 
ATAAGTGGCT CTGAGCTGTG CTTTCCAAGT GGGCAGAAAA TAAAATCTGC TTATCTTCCC 
CAAAGGCAAA TTCACATACC AGCTGTTTTT CAGTCTCCTG CTCATTATAA GCAGACTTTC 
ACATCTTGCC TCATAGAACA TCTAAATATA TTGCTGTTTG GGTTAGCACA AAACCTGCAG 
AAAGCTCTTT CAAAAGTTGA CATATCATTT TATACATCAT TGAAGGGAGA GAAACTGAAA 
AACGCAGAAA ATAATGTACC ATCCTGCCAT CATAGTCAAC CTGCAAAACT TGTCATGGTT 
AAAAAGGAAG GTCCAAATAA GGGTCGTCTC TTTTATACAT GTGATGGACC CAAAGCTGAT 
CGATGTAAAT TCTTTAAATG GCTTGAGGAC GTGACTCCAG GATATTCAAC ACAGGAAGGA 
GCTCGACCTG GCATGGTTTT AAGTGATATT AAGAGTATTG GCTTATATTT AAGAAGTCAA 
AAGATACCAC TTTATGAGGA ATGCCAGCTT TTGGTGAGAA AAGGATTTGA TTTTCAGAGA 
AAACAGTATG GCAAACTAAA GAAGTTTACT ACTGTAAATC CTGAGTTTTA TAATGAACCA 
AAAACCAAAC TTTATCTTAA GCTAAGTCGG AAGGAAAGAT CTTCAGCTTA TAGCAAAAAT 
GATCTTTGGG TGGTTTCAAA AACCCTAGAC TTTGAGCTGG ATACTTTTAT CGCATGTAGT 
GCTTTCTTTG GACCATCATC TATCAATGAG ATAGAAATAC TGCCTTTGAA AGGCTATTTC 
CCTTCTAATT GGCCCACTAA CATGGTTGTC CATGCGTTAT TGGTTTGTAA TGCTAGCACA 
GAACTGACTA CTTTGAAAAA CATTCAGGAC TACTTTAATC CAGCTACTCT ACCTCTAACA 
CAGTACCTGT TAACAACGTC TTCGCCAACT ATAGTTAGTA ACAAAAGAGT CAGTAAGAGA 
AAATTTATCC CACCAGCCTT CACAAATGTC AGTACAAAAT TTGAACTACT CAGCCTAGGA 
GCAACATTGA AGTTAGCTAG TGAGTTGATT CAGGTACACA AGTTAAACAA GGATCAAGCT 
ACAGCTCTAA TTCAAATAGC TCAAATGATG GCATCACATG AAAGCATTGA AGAAGTGAAG 
GAACTGCAAA CTCATACCTT CCCTATCACA ATCATACATG GTGTGTTTGG AGCAGGAAAG 
AGTTACTTGC TGGCAGTGGT GATTTTGTTC TTTGTACAGC TGTTTGAAAA GAGTGAAGCT 
CCCACCATTG GAAATGCAAG GCCGTGGAAA CTTCTGATTT CTTCTTCTAC TAATGTGGCT 
GTTGACAGAG TACTTCTTGG GCTTCTCAGT CTTGGATTTG AAAACTTTAT CAGAGTTGGG 
AGTGTTAGGA AGATTGCCAA ACCAATTTTA CCTTATAGCT TGCATGCTGG CTCAGAAAAT 
GAAAGTGAAC AGTTAAAAGA ACTACATGCA CTAATGAAAG AAGACCTGAC TCCTACGGAA 
AGAGTCTATG TGAGAAAAAG CATTGAGCAG CATAAACTGG GGACCAATAG AACCCTGCTG 
AAGCAGGTTC GAGTAGTTGG AGTTACCTGT GCAGCCTGCC CATTCCCATG CATGAATGAT 
CTTAAATTTC CTGTAGTTGT GCTGGATGAG TGTAGTCAGA TAACTGAACC GGCCTCTCTC 
CTTCCCATTG CAAGGTTTGA GTGTGAAAAG CTGATTCTTG TTGGGGATCC CAAACAGCTA 
CCTCCTACTA TTCAGGGTTC TGATGCAGCT CATGAAAATG GATTGGAACA AACTCTTTTT 
GATCGACTTT GCTTAATGGG TCACAAGCCA ATTCTATTGA GAACTCAATA CCGTTGTCAT 
CCTGCAATCA GTGCTATTGC TAATGATCTG TTTTACAAAG GAGCCCTCAT GAATGGTGTA 
ACAGAAATAG AGCGGAGCCC TTTATTGGAA TGGCTACCAA CCCTGTGTTT TTATAATGTT 
AAAGGACTAG AACAGATAGA AAGAGATAAC AGCTTTCATA ATGTGGCAGA AGCTACGTTT 
ACACTCAAGC TGATTCAATC ACTGATTGCA AGTGGAATAG CAGGCTCTAT GATTGGTGTG 
ATAACATTAT ACAAATCCCA GATGTACAAG CTTTGTCATT TACTCAGTGC TGTGGACTTT 
CACCATCCTG ATATTAAAAC TGTGCAGGTG TCCACAGTAG ATGCTTTTCA GGGAGCTGAA 
AAGGAGATCA TTATTCTGTC CTGTGTAAGG ACAAGACAAG TAGGATTCAT TGATTCAGAA 
AAAAGAATGA ATGTTGCATT GACTAGAGGA AAGAGGCATT TGTTGATTGT GGGAAATTTA 
GCCTGTTTGA GGAAAAATCA ACTTTGGGGA CGAGTGATCC AACACTGCGA AGGAAGGGAA 
GATGGATTGC AACATGCAAA CCAGTATGAA CCACAGCTGA ACCATCTCCT TAAAGATTAT 
TTTGAAAAAC AAGTGGAAGA AAAACAGAAG AAAAAGAGTG AAAAAGAGAA ATCTAAAGAT 
AAATCTCATT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263172.1
CDS	267..6581
Translation	MESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGACLESLFLKCLEVKPGDDLESDRYLITVEEVKVAGAIGIVKQNVNKEAPELNSRTFISSGRSLGCQPSGLKRKFTGFQGPRQVPKKMVIMESGESAASHEAKKTGPTIFSPFCSMPPLFPTVGKKDVNNILADPENIVTYKNRERNAMDFSSVFSPSFQINPEVLCEENYFCSPVNSGNKLSDSLLTNEPVKRDSLASHYSGVSQNIRSKAQILALLKSESSSSCEELNSEMTEHFPQKQPQGSLKIATKPKYLIQQEECAEMKSTENLYYQHQSENTMRNKSRWAMYLSSQSSPIHSSTVDGNDTERKPKAQEDDVNSNLKDLSLQKIIQFVETYAEERKKYNVDQSVGNNDPSWNQEVKLEIPSFNESSSLQVTCSSAENDGILSESDIQEDNKIPFNQNDKGCIKGSVLIKENAQEVNTCGTLEKEYEQSESSLPELKHLQIESSNNSRISDDITDMISESKMDNESLNSIHESLSNVTQPFLEVTFNLNNFETSDTEEESQESNKISQDSESWVKDILVNDGNSCFQKRSENTNCEEIEGEHLPFLTSVSDKPTVTFPVKETLPSQFCDKTYVGFDMGICKTENTGKEIEEYSDTLSNFESFKWTDAVYGDNKEDANKPIQEVRINYDFALPPNKSKGINMNLHIPHIQNQIAENSNLFSEDAQPQPFILGSDLDKNDEHVLPSTSSSDNSVQLLNTNQNHYECIALDKSNTHISNSLFYPLGKKHLISKDTEAHISEPEDLGKIRSPPPDHVEVETAREGKQYWNPRNSSELSGLVNTISILKSLCEHSTALDSLEILKKKNTVFQQGTQQTYEPDSPPEVRKPFITVVSPKSPHLHKDSQQILKEDEVELSEPLQSVQFSSSGSKEETAFQAVIPKQIERKTCDPKPVEFQGHQVKGSATSGVMVRGHSSQLGCSQFPDSTEYENFMTETPELPSTCMQIDFLQVTSPEENISTLSPVSTFSLNSRDEDFMVEFSETSLKARTLPDDLHFLNLEGMKKSRSLENENLQRLSLLSRTQVPLITLPRTDGPPDLDSHSYMINSNTYESSGSPMLNLCEKSAVLSFSIEPEDQNETFFSEESREVNPGDVSLNNISTQSKWLKYQNTSQCNVATPNRVDKRITDGFFAEAVSGMHFRDTSERQSDAVNESSLDSVHLQMIKGMLYQQRQDFSSQDSVSRKKVLSLNLKQTSKTEEIKNVLGGSTCYNYSVKDLQEISGSELCFPSGQKIKSAYLPQRQIHIPAVFQSPAHYKQTFTSCLIEHLNILLFGLAQNLQKALSKVDISFYTSLKGEKLKNAENNVPSCHHSQPAKLVMVKKEGPNKGRLFYTCDGPKADRCKFFKWLEDVTPGYSTQEGARPGMVLSDIKSIGLYLRSQKIPLYEECQLLVRKGFDFQRKQYGKLKKFTTVNPEFYNEPKTKLYLKLSRKERSSAYSKNDLWVVSKTLDFELDTFIACSAFFGPSSINEIEILPLKGYFPSNWPTNMVVHALLVCNASTELTTLKNIQDYFNPATLPLTQYLLTTSSPTIVSNKRVSKRKFIPPAFTNVSTKFELLSLGATLKLASELIQVHKLNKDQATALIQIAQMMASHESIEEVKELQTHTFPITIIHGVFGAGKSYLLAVVILFFVQLFEKSEAPTIGNARPWKLLISSSTNVAVDRVLLGLLSLGFENFIRVGSVRKIAKPILPYSLHAGSENESEQLKELHALMKEDLTPTERVYVRKSIEQHKLGTNRTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPAISAIANDLFYKGALMNGVTEIERSPLLEWLPTLCFYNVKGLEQIERDNSFHNVAEATFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAVDFHHPDIKTVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGKRHLLIVGNLACLRKNQLWGRVIQHCEGREDGLQHANQYEPQLNHLLKDYFEKQVEEKQKKKSEKEKSKDKSHS

Target ORF information:

RefSeq Version	XM_005263115.3
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263115.3

ORF Insert Sequence:

ATGGAAAGCC AAGAATTTAT TGTTCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAATTTT ATATGATGAC 
AAAGGAGCAT GTTTGGAGAG TCTGTTTCTT AAATGCCTTG AGGTGAAACC TGGAGATGAC 
TTAGAAAGTG ATCGATACTT AATCACAGTT GAAGAGGTTA AAGTTGCTGG AGCCATAGGT 
ATTGTTAAGC AGAATGTCAA TAAAGAAGCA CCAGAGTTAA ATTCAAGGAC ATTTATATCC 
TCTGGCCGAT CTCTTGGATG TCAGCCCTCT GGCTTAAAAA GGAAGTTTAC TGGTTTTCAA 
GGACCACGTC AGGTTCCAAA GAAAATGGTT ATTATGGAAA GTGGTGAATC AGCTGCATCA 
CATGAGGCTA AGAAAACTGG CCCTACTATT TTTTCTCCAT TCTGCAGCAT GCCTCCTTTG 
TTTCCTACTG TTGGCAAGAA AGATGTAAAT AATATACTGG CAGACCCTGA GAACATTGTG 
ACTTACAAGA ACAGGGAGAG AAATGCCATG GATTTTTCTT CGGTTTTTTC TCCATCCTTC 
CAGATTAACC CAGAAGTGCT GTGTGAAGAA AATTATTTTT GCTCACCTGT CAATTCTGGA 
AATAAGCTTT CAGACTCTTT ACTGACCAAT GAGCCTGTGA AAAGAGATAG TTTGGCATCT 
CACTATTCAG GAGTTTCACA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTGAAG 
TCCGAATCAT CTAGTTCATG TGAGGAACTA AATTCTGAGA TGACAGAGCA TTTTCCTCAA 
AAACAACCAC AAGGAAGTTT AAAAATTGCT ACTAAACCAA AGTACCTAAT TCAACAGGAA 
GAGTGTGCTG AGATGAAGAG CACAGAAAAT TTATACTACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TATACATTCT 
TCTACTGTAG ATGGGAATGA TACAGAAAGG AAACCCAAGG CCCAGGAAGA TGATGTAAAT 
TCTAATTTGA AAGACCTTTC ATTACAAAAA ATTATACAGT TCGTTGAAAC GTATGCTGAA 
GAGAGGAAAA AGTATAATGT AGACCAGTCA GTCGGTAATA ATGATCCATC CTGGAATCAG 
GAAGTAAAAT TAGAAATTCC TTCATTCAAT GAAAGCAGTA GCTTACAGGT TACTTGTAGC 
AGTGCAGAAA ATGATGGTAT ATTATCAGAA TCTGACATTC AAGAAGATAA TAAAATACCT 
TTTAATCAAA ATGACAAGGG GTGCATTAAA GGATCAGTTC TCATTAAAGA AAATGCTCAG 
GAGGTAAATA CATGTGGAAC ACTGGAAAAG GAGTATGAAC AATCAGAGTC ATCTCTGCCA 
GAACTGAAAC ATCTCCAAAT TGAATCTAGT AATAATTCTA GGATCTCTGA TGACATTACA 
GACATGATTT CTGAAAGTAA AATGGATAAT GAAAGTCTTA ATAGTATTCA TGAATCTCTG 
AGTAATGTAA CACAACCATT TTTGGAGGTA ACTTTTAATC TGAACAATTT TGAGACCAGT 
GACACTGAGG AGGAATCACA GGAAAGCAAC AAAATTTCCC AGGATTCAGA GAGTTGGGTA 
AAGGACATTT TGGTTAATGA TGGCAATTCA TGTTTTCAAA AGAGGTCTGA GAATACAAAC 
TGTGAAGAGA TTGAGGGTGA ACATTTGCCA TTCTTAACAT CTGTTAGTGA CAAACCTACA 
GTGACATTTC CTGTTAAAGA GACTCTGCCA TCACAGTTTT GTGATAAAAC TTATGTGGGT 
TTTGACATGG GAATATGTAA AACTGAAAAC ACAGGAAAAG AAATAGAGGA GTATAGTGAC 
ACATTAAGCA ATTTTGAATC TTTCAAGTGG ACTGATGCTG TATACGGAGA TAATAAAGAA 
GATGCTAATA AACCTATTCA AGAAGTCAGA ATTAACTATG ATTTTGCTTT ACCCCCGAAT 
AAATCTAAAG GTATAAACAT GAATTTACAT ATTCCTCATA TTCAAAACCA GATTGCTGAA 
AACAGTAATC TATTTTCAGA AGATGCTCAA CCTCAGCCTT TTATTTTGGG AAGTGACTTA 
GACAAAAATG ATGAACATGT TTTACCCTCA ACTTCTAGTA GTGACAACAG TGTCCAACTA 
TTAAATACCA ATCAGAATCA CTATGAATGT ATTGCACTTG ATAAATCAAA TACCCACATT 
TCCAATTCTT TGTTTTACCC ACTGGGAAAA AAGCATCTTA TTTCCAAAGA CACAGAAGCA 
CATATATCTG AACCTGAAGA TTTGGGAAAG ATTAGAAGTC CACCCCCTGA CCATGTTGAG 
GTTGAAACTG CCAGAGAAGG CAAACAATAC TGGAATCCTA GAAATTCTTC AGAACTTTCT 
GGATTAGTAA ATACCATTTC TATTTTAAAG TCGCTATGTG AACACAGTAC TGCTTTAGAC 
AGCTTGGAAA TATTGAAAAA GAAAAATACT GTCTTTCAGC AAGGAACTCA ACAGACGTAT 
GAGCCAGATA GCCCTCCTGA AGTGAGGAAA CCATTTATTA CAGTAGTTTC ACCAAAGTCT 
CCTCATCTGC ACAAGGATTC TCAACAGATA CTAAAAGAAG ATGAAGTTGA ACTAAGTGAA 
CCACTTCAGT CTGTGCAGTT CTCTTCCTCG GGAAGTAAAG AAGAGACTGC TTTTCAAGCT 
GTTATTCCTA AACAAATAGA GAGAAAAACC TGTGACCCTA AGCCTGTTGA GTTTCAAGGA 
CATCAAGTAA AAGGATCAGC TACTAGTGGT GTAATGGTCA GAGGACACAG CTCACAGCTA 
GGATGCAGTC AGTTTCCAGA TAGCACTGAG TATGAAAACT TTATGACAGA GACACCAGAG 
TTACCTAGCA CGTGTATGCA GATTGACTTC TTGCAGGTGA CATCACCAGA AGAAAACATC 
TCTACATTGA GCCCTGTTTC TACCTTTTCT TTGAACTCAA GAGATGAAGA CTTCATGGTA 
GAATTCTCTG AGACGTCCCT GAAAGCAAGA ACTTTACCTG ATGATCTTCA TTTTCTCAAC 
TTGGAGGGGA TGAAAAAATC CCGTTCTCTG GAGAATGAGA ACCTTCAAAG GCTTTCATTA 
TTAAGTAGAA CCCAGGTTCC ACTTATTACT TTGCCACGTA CTGATGGGCC ACCTGACTTA 
GACTCTCATT CGTATATGAT CAACTCTAAC ACATACGAGT CTTCTGGCTC CCCCATGCTC 
AATTTGTGTG AAAAGTCAGC AGTTCTTTCG TTTAGCATTG AGCCTGAGGA CCAAAATGAA 
ACCTTTTTCT CTGAAGAATC TAGGGAAGTG AATCCAGGGG ATGTTTCACT TAATAATATA 
TCTACTCAGA GCAAGTGGCT GAAATATCAA AACACATCCC AATGCAACGT GGCTACTCCA 
AACAGAGTTG ATAAGAGAAT AACTGATGGC TTCTTTGCTG AGGCTGTTTC TGGGATGCAT 
TTTAGAGACA CAAGTGAAAG ACAGAGTGAT GCTGTCAATG AAAGCTCTTT AGACTCTGTG 
CATTTGCAAA TGATAAAAGG CATGCTCTAT CAACAGCGGC AGGATTTTAG CAGTCAAGAT 
TCGGTTTCCA GAAAGAAAGT ACTTTCTCTG AATTTAAAGC AGACTTCTAA GACAGAGGAA 
ATTAAAAATG TATTAGGAGG GTCTACCTGC TACAACTACA GTGTAAAGGA TTTACAGGAG 
ATAAGTGGCT CTGAGCTGTG CTTTCCAAGT GGGCAGAAAA TAAAATCTGC TTATCTTCCC 
CAAAGGCAAA TTCACATACC AGCTGTTTTT CAGTCTCCTG CTCATTATAA GCAGACTTTC 
ACATCTTGCC TCATAGAACA TCTAAATATA TTGCTGTTTG GGTTAGCACA AAACCTGCAG 
AAAGCTCTTT CAAAAGTTGA CATATCATTT TATACATCAT TGAAGGGAGA GAAACTGAAA 
AACGCAGAAA ATAATGTACC ATCCTGCCAT CATAGTCAAC CTGCAAAACT TGTCATGGTT 
AAAAAGGAAG GTCCAAATAA GGGTCGTCTC TTTTATACAT GTGATGGACC CAAAGCTGAT 
CGATGTAAAT TCTTTAAATG GCTTGAGGAC GTGACTCCAG GATATTCAAC ACAGGAAGGA 
GCTCGACCTG GCATGGTTTT AAGTGATATT AAGAGTATTG GCTTATATTT AAGAAGTCAA 
AAGATACCAC TTTATGAGGA ATGCCAGCTT TTGGTGAGAA AAGGATTTGA TTTTCAGAGA 
AAACAGTATG GCAAACTAAA GAAGTTTACT ACTGTAAATC CTGAGTTTTA TAATGAACCA 
AAAACCAAAC TTTATCTTAA GCTAAGTCGG AAGGAAAGAT CTTCAGCTTA TAGCAAAAAT 
GATCTTTGGG TGGTTTCAAA AACCCTAGAC TTTGAGCTGG ATACTTTTAT CGCATGTAGT 
GCTTTCTTTG GACCATCATC TATCAATGAG ATAGAAATAC TGCCTTTGAA AGGCTATTTC 
CCTTCTAATT GGCCCACTAA CATGGTTGTC CATGCGTTAT TGGTTTGTAA TGCTAGCACA 
GAACTGACTA CTTTGAAAAA CATTCAGGAC TACTTTAATC CAGCTACTCT ACCTCTAACA 
CAGTACCTGT TAACAACGTC TTCGCCAACT ATAGTTAGTA ACAAAAGAGT CAGTAAGAGA 
AAATTTATCC CACCAGCCTT CACAAATGTC AGTACAAAAT TTGAACTACT CAGCCTAGGA 
GCAACATTGA AGTTAGCTAG TGAGTTGATT CAGGTACACA AGTTAAACAA GGATCAAGCT 
ACAGCTCTAA TTCAAATAGC TCAAATGATG GCATCACATG AAAGCATTGA AGAAGTGAAG 
GAACTGCAAA CTCATACCTT CCCTATCACA ATCATACATG GTGTGTTTGG AGCAGGAAAG 
AGTTACTTGC TGGCAGTGGT GATTTTGTTC TTTGTACAGC TGTTTGAAAA GAGTGAAGCT 
CCCACCATTG GAAATGCAAG GCCGTGGAAA CTTCTGATTT CTTCTTCTAC TAATGTGGCT 
GTTGACAGAG TACTTCTTGG GCTTCTCAGT CTTGGATTTG AAAACTTTAT CAGAGTTGGG 
AGTGTTAGGA AGATTGCCAA ACCAATTTTA CCTTATAGCT TGCATGCTGG CTCAGAAAAT 
GAAAGTGAAC AGTTAAAAGA ACTACATGCA CTAATGAAAG AAGACCTGAC TCCTACGGAA 
AGAGTCTATG TGAGAAAAAG CATTGAGCAG CATAAACTGG GGACCAATAG AACCCTGCTG 
AAGCAGGTTC GAGTAGTTGG AGTTACCTGT GCAGCCTGCC CATTCCCATG CATGAATGAT 
CTTAAATTTC CTGTAGTTGT GCTGGATGAG TGTAGTCAGA TAACTGAACC GGCCTCTCTC 
CTTCCCATTG CAAGGTTTGA GTGTGAAAAG CTGATTCTTG TTGGGGATCC CAAACAGCTA 
CCTCCTACTA TTCAGGGTTC TGATGCAGCT CATGAAAATG GATTGGAACA AACTCTTTTT 
GATCGACTTT GCTTAATGGG TCACAAGCCA ATTCTATTGA GAACTCAATA CCGTTGTCAT 
CCTGCAATCA GTGCTATTGC TAATGATCTG TTTTACAAAG GAGCCCTCAT GAATGGTGTA 
ACAGAAATAG AGCGGAGCCC TTTATTGGAA TGGCTACCAA CCCTGTGTTT TTATAATGTT 
AAAGGACTAG AACAGATAGA AAGAGATAAC AGCTTTCATA ATGTGGCAGA AGCTACGTTT 
ACACTCAAGC TGATTCAATC ACTGATTGCA AGTGGAATAG CAGGCTCTAT GATTGGTGTG 
ATAACATTAT ACAAATCCCA GATGTACAAG CTTTGTCATT TACTCAGTGC TGTGGACTTT 
CACCATCCTG ATATTAAAAC TGTGCAGGTG TCCACAGTAG ATGCTTTTCA GGGAGCTGAA 
AAGGAGATCA TTATTCTGTC CTGTGTAAGG ACAAGACAAG TAGGATTCAT TGATTCAGAA 
AAAAGAATGA ATGTTGCATT GACTAGAGGA AAGAGGCATT TGTTGATTGT GGGAAATTTA 
GCCTGTTTGA GGAAAAATCA ACTTTGGGGA CGAGTGATCC AACACTGCGA AGGAAGGGAA 
GATGGATTGC AACATGCAAA CCAGTATGAA CCACAGCTGA ACCATCTCCT TAAAGATTAT 
TTTGAAAAAC AAGTGGAAGA AAAACAGAAG AAAAAGAGTG AAAAAGAGAA ATCTAAAGAT 
AAATCTCATT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_005263115.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263172.1 Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X1, mRNA.

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CloneID	OHu28391
Clone ID Related Accession (Same CDS sequence)	XM_005263115.3 , XM_005263115.4 , NM_018392.4
Accession Version	XM_005263115.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein ZGRF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005263115.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGCC AAGAATTTAT TGTTCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAATTTT ATATGATGAC 
AAAGGAGCAT GTTTGGAGAG TCTGTTTCTT AAATGCCTTG AGGTGAAACC TGGAGATGAC 
TTAGAAAGTG ATCGATACTT AATCACAGTT GAAGAGGTTA AAGTTGCTGG AGCCATAGGT 
ATTGTTAAGC AGAATGTCAA TAAAGAAGCA CCAGAGTTAA ATTCAAGGAC ATTTATATCC 
TCTGGCCGAT CTCTTGGATG TCAGCCCTCT GGCTTAAAAA GGAAGTTTAC TGGTTTTCAA 
GGACCACGTC AGGTTCCAAA GAAAATGGTT ATTATGGAAA GTGGTGAATC AGCTGCATCA 
CATGAGGCTA AGAAAACTGG CCCTACTATT TTTTCTCCAT TCTGCAGCAT GCCTCCTTTG 
TTTCCTACTG TTGGCAAGAA AGATGTAAAT AATATACTGG CAGACCCTGA GAACATTGTG 
ACTTACAAGA ACAGGGAGAG AAATGCCATG GATTTTTCTT CGGTTTTTTC TCCATCCTTC 
CAGATTAACC CAGAAGTGCT GTGTGAAGAA AATTATTTTT GCTCACCTGT CAATTCTGGA 
AATAAGCTTT CAGACTCTTT ACTGACCAAT GAGCCTGTGA AAAGAGATAG TTTGGCATCT 
CACTATTCAG GAGTTTCACA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTGAAG 
TCCGAATCAT CTAGTTCATG TGAGGAACTA AATTCTGAGA TGACAGAGCA TTTTCCTCAA 
AAACAACCAC AAGGAAGTTT AAAAATTGCT ACTAAACCAA AGTACCTAAT TCAACAGGAA 
GAGTGTGCTG AGATGAAGAG CACAGAAAAT TTATACTACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TATACATTCT 
TCTACTGTAG ATGGGAATGA TACAGAAAGG AAACCCAAGG CCCAGGAAGA TGATGTAAAT 
TCTAATTTGA AAGACCTTTC ATTACAAAAA ATTATACAGT TCGTTGAAAC GTATGCTGAA 
GAGAGGAAAA AGTATAATGT AGACCAGTCA GTCGGTAATA ATGATCCATC CTGGAATCAG 
GAAGTAAAAT TAGAAATTCC TTCATTCAAT GAAAGCAGTA GCTTACAGGT TACTTGTAGC 
AGTGCAGAAA ATGATGGTAT ATTATCAGAA TCTGACATTC AAGAAGATAA TAAAATACCT 
TTTAATCAAA ATGACAAGGG GTGCATTAAA GGATCAGTTC TCATTAAAGA AAATGCTCAG 
GAGGTAAATA CATGTGGAAC ACTGGAAAAG GAGTATGAAC AATCAGAGTC ATCTCTGCCA 
GAACTGAAAC ATCTCCAAAT TGAATCTAGT AATAATTCTA GGATCTCTGA TGACATTACA 
GACATGATTT CTGAAAGTAA AATGGATAAT GAAAGTCTTA ATAGTATTCA TGAATCTCTG 
AGTAATGTAA CACAACCATT TTTGGAGGTA ACTTTTAATC TGAACAATTT TGAGACCAGT 
GACACTGAGG AGGAATCACA GGAAAGCAAC AAAATTTCCC AGGATTCAGA GAGTTGGGTA 
AAGGACATTT TGGTTAATGA TGGCAATTCA TGTTTTCAAA AGAGGTCTGA GAATACAAAC 
TGTGAAGAGA TTGAGGGTGA ACATTTGCCA TTCTTAACAT CTGTTAGTGA CAAACCTACA 
GTGACATTTC CTGTTAAAGA GACTCTGCCA TCACAGTTTT GTGATAAAAC TTATGTGGGT 
TTTGACATGG GAATATGTAA AACTGAAAAC ACAGGAAAAG AAATAGAGGA GTATAGTGAC 
ACATTAAGCA ATTTTGAATC TTTCAAGTGG ACTGATGCTG TATACGGAGA TAATAAAGAA 
GATGCTAATA AACCTATTCA AGAAGTCAGA ATTAACTATG ATTTTGCTTT ACCCCCGAAT 
AAATCTAAAG GTATAAACAT GAATTTACAT ATTCCTCATA TTCAAAACCA GATTGCTGAA 
AACAGTAATC TATTTTCAGA AGATGCTCAA CCTCAGCCTT TTATTTTGGG AAGTGACTTA 
GACAAAAATG ATGAACATGT TTTACCCTCA ACTTCTAGTA GTGACAACAG TGTCCAACTA 
TTAAATACCA ATCAGAATCA CTATGAATGT ATTGCACTTG ATAAATCAAA TACCCACATT 
TCCAATTCTT TGTTTTACCC ACTGGGAAAA AAGCATCTTA TTTCCAAAGA CACAGAAGCA 
CATATATCTG AACCTGAAGA TTTGGGAAAG ATTAGAAGTC CACCCCCTGA CCATGTTGAG 
GTTGAAACTG CCAGAGAAGG CAAACAATAC TGGAATCCTA GAAATTCTTC AGAACTTTCT 
GGATTAGTAA ATACCATTTC TATTTTAAAG TCGCTATGTG AACACAGTAC TGCTTTAGAC 
AGCTTGGAAA TATTGAAAAA GAAAAATACT GTCTTTCAGC AAGGAACTCA ACAGACGTAT 
GAGCCAGATA GCCCTCCTGA AGTGAGGAAA CCATTTATTA CAGTAGTTTC ACCAAAGTCT 
CCTCATCTGC ACAAGGATTC TCAACAGATA CTAAAAGAAG ATGAAGTTGA ACTAAGTGAA 
CCACTTCAGT CTGTGCAGTT CTCTTCCTCG GGAAGTAAAG AAGAGACTGC TTTTCAAGCT 
GTTATTCCTA AACAAATAGA GAGAAAAACC TGTGACCCTA AGCCTGTTGA GTTTCAAGGA 
CATCAAGTAA AAGGATCAGC TACTAGTGGT GTAATGGTCA GAGGACACAG CTCACAGCTA 
GGATGCAGTC AGTTTCCAGA TAGCACTGAG TATGAAAACT TTATGACAGA GACACCAGAG 
TTACCTAGCA CGTGTATGCA GATTGACTTC TTGCAGGTGA CATCACCAGA AGAAAACATC 
TCTACATTGA GCCCTGTTTC TACCTTTTCT TTGAACTCAA GAGATGAAGA CTTCATGGTA 
GAATTCTCTG AGACGTCCCT GAAAGCAAGA ACTTTACCTG ATGATCTTCA TTTTCTCAAC 
TTGGAGGGGA TGAAAAAATC CCGTTCTCTG GAGAATGAGA ACCTTCAAAG GCTTTCATTA 
TTAAGTAGAA CCCAGGTTCC ACTTATTACT TTGCCACGTA CTGATGGGCC ACCTGACTTA 
GACTCTCATT CGTATATGAT CAACTCTAAC ACATACGAGT CTTCTGGCTC CCCCATGCTC 
AATTTGTGTG AAAAGTCAGC AGTTCTTTCG TTTAGCATTG AGCCTGAGGA CCAAAATGAA 
ACCTTTTTCT CTGAAGAATC TAGGGAAGTG AATCCAGGGG ATGTTTCACT TAATAATATA 
TCTACTCAGA GCAAGTGGCT GAAATATCAA AACACATCCC AATGCAACGT GGCTACTCCA 
AACAGAGTTG ATAAGAGAAT AACTGATGGC TTCTTTGCTG AGGCTGTTTC TGGGATGCAT 
TTTAGAGACA CAAGTGAAAG ACAGAGTGAT GCTGTCAATG AAAGCTCTTT AGACTCTGTG 
CATTTGCAAA TGATAAAAGG CATGCTCTAT CAACAGCGGC AGGATTTTAG CAGTCAAGAT 
TCGGTTTCCA GAAAGAAAGT ACTTTCTCTG AATTTAAAGC AGACTTCTAA GACAGAGGAA 
ATTAAAAATG TATTAGGAGG GTCTACCTGC TACAACTACA GTGTAAAGGA TTTACAGGAG 
ATAAGTGGCT CTGAGCTGTG CTTTCCAAGT GGGCAGAAAA TAAAATCTGC TTATCTTCCC 
CAAAGGCAAA TTCACATACC AGCTGTTTTT CAGTCTCCTG CTCATTATAA GCAGACTTTC 
ACATCTTGCC TCATAGAACA TCTAAATATA TTGCTGTTTG GGTTAGCACA AAACCTGCAG 
AAAGCTCTTT CAAAAGTTGA CATATCATTT TATACATCAT TGAAGGGAGA GAAACTGAAA 
AACGCAGAAA ATAATGTACC ATCCTGCCAT CATAGTCAAC CTGCAAAACT TGTCATGGTT 
AAAAAGGAAG GTCCAAATAA GGGTCGTCTC TTTTATACAT GTGATGGACC CAAAGCTGAT 
CGATGTAAAT TCTTTAAATG GCTTGAGGAC GTGACTCCAG GATATTCAAC ACAGGAAGGA 
GCTCGACCTG GCATGGTTTT AAGTGATATT AAGAGTATTG GCTTATATTT AAGAAGTCAA 
AAGATACCAC TTTATGAGGA ATGCCAGCTT TTGGTGAGAA AAGGATTTGA TTTTCAGAGA 
AAACAGTATG GCAAACTAAA GAAGTTTACT ACTGTAAATC CTGAGTTTTA TAATGAACCA 
AAAACCAAAC TTTATCTTAA GCTAAGTCGG AAGGAAAGAT CTTCAGCTTA TAGCAAAAAT 
GATCTTTGGG TGGTTTCAAA AACCCTAGAC TTTGAGCTGG ATACTTTTAT CGCATGTAGT 
GCTTTCTTTG GACCATCATC TATCAATGAG ATAGAAATAC TGCCTTTGAA AGGCTATTTC 
CCTTCTAATT GGCCCACTAA CATGGTTGTC CATGCGTTAT TGGTTTGTAA TGCTAGCACA 
GAACTGACTA CTTTGAAAAA CATTCAGGAC TACTTTAATC CAGCTACTCT ACCTCTAACA 
CAGTACCTGT TAACAACGTC TTCGCCAACT ATAGTTAGTA ACAAAAGAGT CAGTAAGAGA 
AAATTTATCC CACCAGCCTT CACAAATGTC AGTACAAAAT TTGAACTACT CAGCCTAGGA 
GCAACATTGA AGTTAGCTAG TGAGTTGATT CAGGTACACA AGTTAAACAA GGATCAAGCT 
ACAGCTCTAA TTCAAATAGC TCAAATGATG GCATCACATG AAAGCATTGA AGAAGTGAAG 
GAACTGCAAA CTCATACCTT CCCTATCACA ATCATACATG GTGTGTTTGG AGCAGGAAAG 
AGTTACTTGC TGGCAGTGGT GATTTTGTTC TTTGTACAGC TGTTTGAAAA GAGTGAAGCT 
CCCACCATTG GAAATGCAAG GCCGTGGAAA CTTCTGATTT CTTCTTCTAC TAATGTGGCT 
GTTGACAGAG TACTTCTTGG GCTTCTCAGT CTTGGATTTG AAAACTTTAT CAGAGTTGGG 
AGTGTTAGGA AGATTGCCAA ACCAATTTTA CCTTATAGCT TGCATGCTGG CTCAGAAAAT 
GAAAGTGAAC AGTTAAAAGA ACTACATGCA CTAATGAAAG AAGACCTGAC TCCTACGGAA 
AGAGTCTATG TGAGAAAAAG CATTGAGCAG CATAAACTGG GGACCAATAG AACCCTGCTG 
AAGCAGGTTC GAGTAGTTGG AGTTACCTGT GCAGCCTGCC CATTCCCATG CATGAATGAT 
CTTAAATTTC CTGTAGTTGT GCTGGATGAG TGTAGTCAGA TAACTGAACC GGCCTCTCTC 
CTTCCCATTG CAAGGTTTGA GTGTGAAAAG CTGATTCTTG TTGGGGATCC CAAACAGCTA 
CCTCCTACTA TTCAGGGTTC TGATGCAGCT CATGAAAATG GATTGGAACA AACTCTTTTT 
GATCGACTTT GCTTAATGGG TCACAAGCCA ATTCTATTGA GAACTCAATA CCGTTGTCAT 
CCTGCAATCA GTGCTATTGC TAATGATCTG TTTTACAAAG GAGCCCTCAT GAATGGTGTA 
ACAGAAATAG AGCGGAGCCC TTTATTGGAA TGGCTACCAA CCCTGTGTTT TTATAATGTT 
AAAGGACTAG AACAGATAGA AAGAGATAAC AGCTTTCATA ATGTGGCAGA AGCTACGTTT 
ACACTCAAGC TGATTCAATC ACTGATTGCA AGTGGAATAG CAGGCTCTAT GATTGGTGTG 
ATAACATTAT ACAAATCCCA GATGTACAAG CTTTGTCATT TACTCAGTGC TGTGGACTTT 
CACCATCCTG ATATTAAAAC TGTGCAGGTG TCCACAGTAG ATGCTTTTCA GGGAGCTGAA 
AAGGAGATCA TTATTCTGTC CTGTGTAAGG ACAAGACAAG TAGGATTCAT TGATTCAGAA 
AAAAGAATGA ATGTTGCATT GACTAGAGGA AAGAGGCATT TGTTGATTGT GGGAAATTTA 
GCCTGTTTGA GGAAAAATCA ACTTTGGGGA CGAGTGATCC AACACTGCGA AGGAAGGGAA 
GATGGATTGC AACATGCAAA CCAGTATGAA CCACAGCTGA ACCATCTCCT TAAAGATTAT 
TTTGAAAAAC AAGTGGAAGA AAAACAGAAG AAAAAGAGTG AAAAAGAGAA ATCTAAAGAT 
AAATCTCATT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263172.1
CDS	271..6585
Translation	MESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGACLESLFLKCLEVKPGDDLESDRYLITVEEVKVAGAIGIVKQNVNKEAPELNSRTFISSGRSLGCQPSGLKRKFTGFQGPRQVPKKMVIMESGESAASHEAKKTGPTIFSPFCSMPPLFPTVGKKDVNNILADPENIVTYKNRERNAMDFSSVFSPSFQINPEVLCEENYFCSPVNSGNKLSDSLLTNEPVKRDSLASHYSGVSQNIRSKAQILALLKSESSSSCEELNSEMTEHFPQKQPQGSLKIATKPKYLIQQEECAEMKSTENLYYQHQSENTMRNKSRWAMYLSSQSSPIHSSTVDGNDTERKPKAQEDDVNSNLKDLSLQKIIQFVETYAEERKKYNVDQSVGNNDPSWNQEVKLEIPSFNESSSLQVTCSSAENDGILSESDIQEDNKIPFNQNDKGCIKGSVLIKENAQEVNTCGTLEKEYEQSESSLPELKHLQIESSNNSRISDDITDMISESKMDNESLNSIHESLSNVTQPFLEVTFNLNNFETSDTEEESQESNKISQDSESWVKDILVNDGNSCFQKRSENTNCEEIEGEHLPFLTSVSDKPTVTFPVKETLPSQFCDKTYVGFDMGICKTENTGKEIEEYSDTLSNFESFKWTDAVYGDNKEDANKPIQEVRINYDFALPPNKSKGINMNLHIPHIQNQIAENSNLFSEDAQPQPFILGSDLDKNDEHVLPSTSSSDNSVQLLNTNQNHYECIALDKSNTHISNSLFYPLGKKHLISKDTEAHISEPEDLGKIRSPPPDHVEVETAREGKQYWNPRNSSELSGLVNTISILKSLCEHSTALDSLEILKKKNTVFQQGTQQTYEPDSPPEVRKPFITVVSPKSPHLHKDSQQILKEDEVELSEPLQSVQFSSSGSKEETAFQAVIPKQIERKTCDPKPVEFQGHQVKGSATSGVMVRGHSSQLGCSQFPDSTEYENFMTETPELPSTCMQIDFLQVTSPEENISTLSPVSTFSLNSRDEDFMVEFSETSLKARTLPDDLHFLNLEGMKKSRSLENENLQRLSLLSRTQVPLITLPRTDGPPDLDSHSYMINSNTYESSGSPMLNLCEKSAVLSFSIEPEDQNETFFSEESREVNPGDVSLNNISTQSKWLKYQNTSQCNVATPNRVDKRITDGFFAEAVSGMHFRDTSERQSDAVNESSLDSVHLQMIKGMLYQQRQDFSSQDSVSRKKVLSLNLKQTSKTEEIKNVLGGSTCYNYSVKDLQEISGSELCFPSGQKIKSAYLPQRQIHIPAVFQSPAHYKQTFTSCLIEHLNILLFGLAQNLQKALSKVDISFYTSLKGEKLKNAENNVPSCHHSQPAKLVMVKKEGPNKGRLFYTCDGPKADRCKFFKWLEDVTPGYSTQEGARPGMVLSDIKSIGLYLRSQKIPLYEECQLLVRKGFDFQRKQYGKLKKFTTVNPEFYNEPKTKLYLKLSRKERSSAYSKNDLWVVSKTLDFELDTFIACSAFFGPSSINEIEILPLKGYFPSNWPTNMVVHALLVCNASTELTTLKNIQDYFNPATLPLTQYLLTTSSPTIVSNKRVSKRKFIPPAFTNVSTKFELLSLGATLKLASELIQVHKLNKDQATALIQIAQMMASHESIEEVKELQTHTFPITIIHGVFGAGKSYLLAVVILFFVQLFEKSEAPTIGNARPWKLLISSSTNVAVDRVLLGLLSLGFENFIRVGSVRKIAKPILPYSLHAGSENESEQLKELHALMKEDLTPTERVYVRKSIEQHKLGTNRTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPAISAIANDLFYKGALMNGVTEIERSPLLEWLPTLCFYNVKGLEQIERDNSFHNVAEATFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAVDFHHPDIKTVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGKRHLLIVGNLACLRKNQLWGRVIQHCEGREDGLQHANQYEPQLNHLLKDYFEKQVEEKQKKKSEKEKSKDKSHS

Target ORF information:

RefSeq Version	XM_005263115.4
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263115.4

ORF Insert Sequence:

ATGGAAAGCC AAGAATTTAT TGTTCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAATTTT ATATGATGAC 
AAAGGAGCAT GTTTGGAGAG TCTGTTTCTT AAATGCCTTG AGGTGAAACC TGGAGATGAC 
TTAGAAAGTG ATCGATACTT AATCACAGTT GAAGAGGTTA AAGTTGCTGG AGCCATAGGT 
ATTGTTAAGC AGAATGTCAA TAAAGAAGCA CCAGAGTTAA ATTCAAGGAC ATTTATATCC 
TCTGGCCGAT CTCTTGGATG TCAGCCCTCT GGCTTAAAAA GGAAGTTTAC TGGTTTTCAA 
GGACCACGTC AGGTTCCAAA GAAAATGGTT ATTATGGAAA GTGGTGAATC AGCTGCATCA 
CATGAGGCTA AGAAAACTGG CCCTACTATT TTTTCTCCAT TCTGCAGCAT GCCTCCTTTG 
TTTCCTACTG TTGGCAAGAA AGATGTAAAT AATATACTGG CAGACCCTGA GAACATTGTG 
ACTTACAAGA ACAGGGAGAG AAATGCCATG GATTTTTCTT CGGTTTTTTC TCCATCCTTC 
CAGATTAACC CAGAAGTGCT GTGTGAAGAA AATTATTTTT GCTCACCTGT CAATTCTGGA 
AATAAGCTTT CAGACTCTTT ACTGACCAAT GAGCCTGTGA AAAGAGATAG TTTGGCATCT 
CACTATTCAG GAGTTTCACA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTGAAG 
TCCGAATCAT CTAGTTCATG TGAGGAACTA AATTCTGAGA TGACAGAGCA TTTTCCTCAA 
AAACAACCAC AAGGAAGTTT AAAAATTGCT ACTAAACCAA AGTACCTAAT TCAACAGGAA 
GAGTGTGCTG AGATGAAGAG CACAGAAAAT TTATACTACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TATACATTCT 
TCTACTGTAG ATGGGAATGA TACAGAAAGG AAACCCAAGG CCCAGGAAGA TGATGTAAAT 
TCTAATTTGA AAGACCTTTC ATTACAAAAA ATTATACAGT TCGTTGAAAC GTATGCTGAA 
GAGAGGAAAA AGTATAATGT AGACCAGTCA GTCGGTAATA ATGATCCATC CTGGAATCAG 
GAAGTAAAAT TAGAAATTCC TTCATTCAAT GAAAGCAGTA GCTTACAGGT TACTTGTAGC 
AGTGCAGAAA ATGATGGTAT ATTATCAGAA TCTGACATTC AAGAAGATAA TAAAATACCT 
TTTAATCAAA ATGACAAGGG GTGCATTAAA GGATCAGTTC TCATTAAAGA AAATGCTCAG 
GAGGTAAATA CATGTGGAAC ACTGGAAAAG GAGTATGAAC AATCAGAGTC ATCTCTGCCA 
GAACTGAAAC ATCTCCAAAT TGAATCTAGT AATAATTCTA GGATCTCTGA TGACATTACA 
GACATGATTT CTGAAAGTAA AATGGATAAT GAAAGTCTTA ATAGTATTCA TGAATCTCTG 
AGTAATGTAA CACAACCATT TTTGGAGGTA ACTTTTAATC TGAACAATTT TGAGACCAGT 
GACACTGAGG AGGAATCACA GGAAAGCAAC AAAATTTCCC AGGATTCAGA GAGTTGGGTA 
AAGGACATTT TGGTTAATGA TGGCAATTCA TGTTTTCAAA AGAGGTCTGA GAATACAAAC 
TGTGAAGAGA TTGAGGGTGA ACATTTGCCA TTCTTAACAT CTGTTAGTGA CAAACCTACA 
GTGACATTTC CTGTTAAAGA GACTCTGCCA TCACAGTTTT GTGATAAAAC TTATGTGGGT 
TTTGACATGG GAATATGTAA AACTGAAAAC ACAGGAAAAG AAATAGAGGA GTATAGTGAC 
ACATTAAGCA ATTTTGAATC TTTCAAGTGG ACTGATGCTG TATACGGAGA TAATAAAGAA 
GATGCTAATA AACCTATTCA AGAAGTCAGA ATTAACTATG ATTTTGCTTT ACCCCCGAAT 
AAATCTAAAG GTATAAACAT GAATTTACAT ATTCCTCATA TTCAAAACCA GATTGCTGAA 
AACAGTAATC TATTTTCAGA AGATGCTCAA CCTCAGCCTT TTATTTTGGG AAGTGACTTA 
GACAAAAATG ATGAACATGT TTTACCCTCA ACTTCTAGTA GTGACAACAG TGTCCAACTA 
TTAAATACCA ATCAGAATCA CTATGAATGT ATTGCACTTG ATAAATCAAA TACCCACATT 
TCCAATTCTT TGTTTTACCC ACTGGGAAAA AAGCATCTTA TTTCCAAAGA CACAGAAGCA 
CATATATCTG AACCTGAAGA TTTGGGAAAG ATTAGAAGTC CACCCCCTGA CCATGTTGAG 
GTTGAAACTG CCAGAGAAGG CAAACAATAC TGGAATCCTA GAAATTCTTC AGAACTTTCT 
GGATTAGTAA ATACCATTTC TATTTTAAAG TCGCTATGTG AACACAGTAC TGCTTTAGAC 
AGCTTGGAAA TATTGAAAAA GAAAAATACT GTCTTTCAGC AAGGAACTCA ACAGACGTAT 
GAGCCAGATA GCCCTCCTGA AGTGAGGAAA CCATTTATTA CAGTAGTTTC ACCAAAGTCT 
CCTCATCTGC ACAAGGATTC TCAACAGATA CTAAAAGAAG ATGAAGTTGA ACTAAGTGAA 
CCACTTCAGT CTGTGCAGTT CTCTTCCTCG GGAAGTAAAG AAGAGACTGC TTTTCAAGCT 
GTTATTCCTA AACAAATAGA GAGAAAAACC TGTGACCCTA AGCCTGTTGA GTTTCAAGGA 
CATCAAGTAA AAGGATCAGC TACTAGTGGT GTAATGGTCA GAGGACACAG CTCACAGCTA 
GGATGCAGTC AGTTTCCAGA TAGCACTGAG TATGAAAACT TTATGACAGA GACACCAGAG 
TTACCTAGCA CGTGTATGCA GATTGACTTC TTGCAGGTGA CATCACCAGA AGAAAACATC 
TCTACATTGA GCCCTGTTTC TACCTTTTCT TTGAACTCAA GAGATGAAGA CTTCATGGTA 
GAATTCTCTG AGACGTCCCT GAAAGCAAGA ACTTTACCTG ATGATCTTCA TTTTCTCAAC 
TTGGAGGGGA TGAAAAAATC CCGTTCTCTG GAGAATGAGA ACCTTCAAAG GCTTTCATTA 
TTAAGTAGAA CCCAGGTTCC ACTTATTACT TTGCCACGTA CTGATGGGCC ACCTGACTTA 
GACTCTCATT CGTATATGAT CAACTCTAAC ACATACGAGT CTTCTGGCTC CCCCATGCTC 
AATTTGTGTG AAAAGTCAGC AGTTCTTTCG TTTAGCATTG AGCCTGAGGA CCAAAATGAA 
ACCTTTTTCT CTGAAGAATC TAGGGAAGTG AATCCAGGGG ATGTTTCACT TAATAATATA 
TCTACTCAGA GCAAGTGGCT GAAATATCAA AACACATCCC AATGCAACGT GGCTACTCCA 
AACAGAGTTG ATAAGAGAAT AACTGATGGC TTCTTTGCTG AGGCTGTTTC TGGGATGCAT 
TTTAGAGACA CAAGTGAAAG ACAGAGTGAT GCTGTCAATG AAAGCTCTTT AGACTCTGTG 
CATTTGCAAA TGATAAAAGG CATGCTCTAT CAACAGCGGC AGGATTTTAG CAGTCAAGAT 
TCGGTTTCCA GAAAGAAAGT ACTTTCTCTG AATTTAAAGC AGACTTCTAA GACAGAGGAA 
ATTAAAAATG TATTAGGAGG GTCTACCTGC TACAACTACA GTGTAAAGGA TTTACAGGAG 
ATAAGTGGCT CTGAGCTGTG CTTTCCAAGT GGGCAGAAAA TAAAATCTGC TTATCTTCCC 
CAAAGGCAAA TTCACATACC AGCTGTTTTT CAGTCTCCTG CTCATTATAA GCAGACTTTC 
ACATCTTGCC TCATAGAACA TCTAAATATA TTGCTGTTTG GGTTAGCACA AAACCTGCAG 
AAAGCTCTTT CAAAAGTTGA CATATCATTT TATACATCAT TGAAGGGAGA GAAACTGAAA 
AACGCAGAAA ATAATGTACC ATCCTGCCAT CATAGTCAAC CTGCAAAACT TGTCATGGTT 
AAAAAGGAAG GTCCAAATAA GGGTCGTCTC TTTTATACAT GTGATGGACC CAAAGCTGAT 
CGATGTAAAT TCTTTAAATG GCTTGAGGAC GTGACTCCAG GATATTCAAC ACAGGAAGGA 
GCTCGACCTG GCATGGTTTT AAGTGATATT AAGAGTATTG GCTTATATTT AAGAAGTCAA 
AAGATACCAC TTTATGAGGA ATGCCAGCTT TTGGTGAGAA AAGGATTTGA TTTTCAGAGA 
AAACAGTATG GCAAACTAAA GAAGTTTACT ACTGTAAATC CTGAGTTTTA TAATGAACCA 
AAAACCAAAC TTTATCTTAA GCTAAGTCGG AAGGAAAGAT CTTCAGCTTA TAGCAAAAAT 
GATCTTTGGG TGGTTTCAAA AACCCTAGAC TTTGAGCTGG ATACTTTTAT CGCATGTAGT 
GCTTTCTTTG GACCATCATC TATCAATGAG ATAGAAATAC TGCCTTTGAA AGGCTATTTC 
CCTTCTAATT GGCCCACTAA CATGGTTGTC CATGCGTTAT TGGTTTGTAA TGCTAGCACA 
GAACTGACTA CTTTGAAAAA CATTCAGGAC TACTTTAATC CAGCTACTCT ACCTCTAACA 
CAGTACCTGT TAACAACGTC TTCGCCAACT ATAGTTAGTA ACAAAAGAGT CAGTAAGAGA 
AAATTTATCC CACCAGCCTT CACAAATGTC AGTACAAAAT TTGAACTACT CAGCCTAGGA 
GCAACATTGA AGTTAGCTAG TGAGTTGATT CAGGTACACA AGTTAAACAA GGATCAAGCT 
ACAGCTCTAA TTCAAATAGC TCAAATGATG GCATCACATG AAAGCATTGA AGAAGTGAAG 
GAACTGCAAA CTCATACCTT CCCTATCACA ATCATACATG GTGTGTTTGG AGCAGGAAAG 
AGTTACTTGC TGGCAGTGGT GATTTTGTTC TTTGTACAGC TGTTTGAAAA GAGTGAAGCT 
CCCACCATTG GAAATGCAAG GCCGTGGAAA CTTCTGATTT CTTCTTCTAC TAATGTGGCT 
GTTGACAGAG TACTTCTTGG GCTTCTCAGT CTTGGATTTG AAAACTTTAT CAGAGTTGGG 
AGTGTTAGGA AGATTGCCAA ACCAATTTTA CCTTATAGCT TGCATGCTGG CTCAGAAAAT 
GAAAGTGAAC AGTTAAAAGA ACTACATGCA CTAATGAAAG AAGACCTGAC TCCTACGGAA 
AGAGTCTATG TGAGAAAAAG CATTGAGCAG CATAAACTGG GGACCAATAG AACCCTGCTG 
AAGCAGGTTC GAGTAGTTGG AGTTACCTGT GCAGCCTGCC CATTCCCATG CATGAATGAT 
CTTAAATTTC CTGTAGTTGT GCTGGATGAG TGTAGTCAGA TAACTGAACC GGCCTCTCTC 
CTTCCCATTG CAAGGTTTGA GTGTGAAAAG CTGATTCTTG TTGGGGATCC CAAACAGCTA 
CCTCCTACTA TTCAGGGTTC TGATGCAGCT CATGAAAATG GATTGGAACA AACTCTTTTT 
GATCGACTTT GCTTAATGGG TCACAAGCCA ATTCTATTGA GAACTCAATA CCGTTGTCAT 
CCTGCAATCA GTGCTATTGC TAATGATCTG TTTTACAAAG GAGCCCTCAT GAATGGTGTA 
ACAGAAATAG AGCGGAGCCC TTTATTGGAA TGGCTACCAA CCCTGTGTTT TTATAATGTT 
AAAGGACTAG AACAGATAGA AAGAGATAAC AGCTTTCATA ATGTGGCAGA AGCTACGTTT 
ACACTCAAGC TGATTCAATC ACTGATTGCA AGTGGAATAG CAGGCTCTAT GATTGGTGTG 
ATAACATTAT ACAAATCCCA GATGTACAAG CTTTGTCATT TACTCAGTGC TGTGGACTTT 
CACCATCCTG ATATTAAAAC TGTGCAGGTG TCCACAGTAG ATGCTTTTCA GGGAGCTGAA 
AAGGAGATCA TTATTCTGTC CTGTGTAAGG ACAAGACAAG TAGGATTCAT TGATTCAGAA 
AAAAGAATGA ATGTTGCATT GACTAGAGGA AAGAGGCATT TGTTGATTGT GGGAAATTTA 
GCCTGTTTGA GGAAAAATCA ACTTTGGGGA CGAGTGATCC AACACTGCGA AGGAAGGGAA 
GATGGATTGC AACATGCAAA CCAGTATGAA CCACAGCTGA ACCATCTCCT TAAAGATTAT 
TTTGAAAAAC AAGTGGAAGA AAAACAGAAG AAAAAGAGTG AAAAAGAGAA ATCTAAAGAT 
AAATCTCATT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_024454130.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309898.1 Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OHu95282
Clone ID Related Accession (Same CDS sequence)	XM_024454130.1 , XM_017008373.1 , XM_017008372.1 , XM_024454129.1
Accession Version	XM_024454130.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein ZGRF1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTTCA TTTTCTCAAC TTGGAGGGAG ATAAGTGGCT CTGAGCTGTG CTTTCCAAGT 
GGGCAGAAAA TAAAATCTGC TTATCTTCCC CAAAGGCAAA TTCACATACC AGCTGTTTTT 
CAGTCTCCTG CTCATTATAA GCAGACTTTC ACATCTTGCC TCATAGAACA TCTAAATATA 
TTGCTGTTTG GGTTAGCACA AAACCTGCAG AAAGCTCTTT CAAAAGTTGA CATATCATTT 
TATACATCAT TGAAGGGAGA GAAACTGAAA AACGCAGAAA ATAATGTACC ATCCTGCCAT 
CATAGTCAAC CTGCAAAACT TGTCATGGTT AAAAAGGAAG GTCCAAATAA GGGTCGTCTC 
TTTTATACAT GTGATGGACC CAAAGCTGAT CGATGTAAAT TCTTTAAATG GCTTGAGGAC 
GTGACTCCAG GATATTCAAC ACAGGAAGGA GCTCGACCTG GCATGGTTTT AAGTGATATT 
AAGAGTATTG GCTTATATTT AAGAAGTCAA AAGATACCAC TTTATGAGGA ATGCCAGCTT 
TTGGTGAGAA AAGGATTTGA TTTTCAGAGA AAACAGTATG GCAAACTAAA GAAGTTTACT 
ACTGTAAATC CTGAGTTTTA TAATGAACCA AAAACCAAAC TTTATCTTAA GCTAAGTCGG 
AAGGAAAGAT CTTCAGCTTA TAGCAAAAAT GATCTTTGGG TGGTTTCAAA AACCCTAGAC 
TTTGAGCTGG ATACTTTTAT CGCATGTAGT GCTTTCTTTG GACCATCATC TATCAATGAG 
ATAGAAATAC TGCCTTTGAA AGGCTATTTC CCTTCTAATT GGCCCACTAA CATGGTTGTC 
CATGCGTTAT TGGTTTGTAA TGCTAGCACA GAACTGACTA CTTTGAAAAA CATTCAGGAC 
TACTTTAATC CAGCTACTCT ACCTCTAACA CAGTACCTGT TAACAACGTC TTCGCCAACT 
ATAGTTAGTA ACAAAAGAGT CAGTAAGAGA AAATTTATCC CACCAGCCTT CACAAATGTC 
AGTACAAAAT TTGAACTACT CAGCCTAGGA GCAACATTGA AGTTAGCTAG TGAGTTGATT 
CAGGTACACA AGTTAAACAA GGATCAAGCT ACAGCTCTAA TTCAAATAGC TCAAATGATG 
GCATCACATG AAAGCATTGA AGAAGTGAAG GAACTGCAAA CTCATACCTT CCCTATCACA 
ATCATACATG GTGTGTTTGG AGCAGGAAAG AGTTACTTGC TGGCAGTGGT GATTTTGTTC 
TTTGTACAGC TGTTTGAAAA GAGTGAAGCT CCCACCATTG GAAATGCAAG GCCGTGGAAA 
CTTCTGATTT CTTCTTCTAC TAATGTGGCT GTTGACAGAG TACTTCTTGG GCTTCTCAGT 
CTTGGATTTG AAAACTTTAT CAGAGTTGGG AGTGTTAGGA AGATTGCCAA ACCAATTTTA 
CCTTATAGCT TGCATGCTGG CTCAGAAAAT GAAAGTGAAC AGTTAAAAGA ACTACATGCA 
CTAATGAAAG AAGACCTGAC TCCTACGGAA AGAGTCTATG TGAGAAAAAG CATTGAGCAG 
CATAAACTGG GGACCAATAG AACCCTGCTG AAGCAGGTTC GAGTAGTTGG AGTTACCTGT 
GCAGCCTGCC CATTCCCATG CATGAATGAT CTTAAATTTC CTGTAGTTGT GCTGGATGAG 
TGTAGTCAGA TAACTGAACC GGCCTCTCTC CTTCCCATTG CAAGGTTTGA GTGTGAAAAG 
CTGATTCTTG TTGGGGATCC CAAACAGCTA CCTCCTACTA TTCAGGGTTC TGATGCAGCT 
CATGAAAATG GATTGGAACA AACTCTTTTT GATCGACTTT GCTTAATGGG TCACAAGCCA 
ATTCTATTGA GAACTCAATA CCGTTGTCAT CCTGCAATCA GTGCTATTGC TAATGATCTG 
TTTTACAAAG GAGCCCTCAT GAATGGTGTA ACAGAAATAG AGCGGAGCCC TTTATTGGAA 
TGGCTACCAA CCCTGTGTTT TTATAATGTT AAAGGACTAG AACAGATAGA AAGAGATAAC 
AGCTTTCATA ATGTGGCAGA AGCTACGTTT ACACTCAAGC TGATTCAATC ACTGATTGCA 
AGTGGAATAG CAGGCTCTAT GATTGGTGTG ATAACATTAT ACAAATCCCA GATGTACAAG 
CTTTGTCATT TACTCAGTGC TGTGGACTTT CACCATCCTG ATATTAAAAC TGTGCAGGTG 
TCCACAGTAG ATGCTTTTCA GGGAGCTGAA AAGGAGATCA TTATTCTGTC CTGTGTAAGG 
ACAAGACAAG TAGGATTCAT TGATTCAGAA AAAAGAATGA ATGTTGCATT GACTAGAGGA 
AAGAGGCATT TGTTGATTGT GGGAAATTTA GCCTGTTTGA GGAAAAATCA ACTTTGGGGA 
CGAGTGATCC AACACTGCGA AGGAAGGGAA GATGGATTGC AACATGCAAA CCAGTATGAA 
CCACAGCTGA ACCATCTCCT TAAAGATTAT TTTGAAAAAC AAGTGGAAGA AAAACAGAAG 
AAAAAGAGTG AAAAAGAGAA ATCTAAAGAT AAATCTCATT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309898.1
CDS	958..3522
Translation	MIFIFSTWREISGSELCFPSGQKIKSAYLPQRQIHIPAVFQSPAHYKQTFTSCLIEHLNILLFGLAQNLQKALSKVDISFYTSLKGEKLKNAENNVPSCHHSQPAKLVMVKKEGPNKGRLFYTCDGPKADRCKFFKWLEDVTPGYSTQEGARPGMVLSDIKSIGLYLRSQKIPLYEECQLLVRKGFDFQRKQYGKLKKFTTVNPEFYNEPKTKLYLKLSRKERSSAYSKNDLWVVSKTLDFELDTFIACSAFFGPSSINEIEILPLKGYFPSNWPTNMVVHALLVCNASTELTTLKNIQDYFNPATLPLTQYLLTTSSPTIVSNKRVSKRKFIPPAFTNVSTKFELLSLGATLKLASELIQVHKLNKDQATALIQIAQMMASHESIEEVKELQTHTFPITIIHGVFGAGKSYLLAVVILFFVQLFEKSEAPTIGNARPWKLLISSSTNVAVDRVLLGLLSLGFENFIRVGSVRKIAKPILPYSLHAGSENESEQLKELHALMKEDLTPTERVYVRKSIEQHKLGTNRTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPAISAIANDLFYKGALMNGVTEIERSPLLEWLPTLCFYNVKGLEQIERDNSFHNVAEATFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAVDFHHPDIKTVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGKRHLLIVGNLACLRKNQLWGRVIQHCEGREDGLQHANQYEPQLNHLLKDYFEKQVEEKQKKKSEKEKSKDKSHS

Target ORF information:

RefSeq Version	XM_024454130.1
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024454130.1

ORF Insert Sequence:

ATGATCTTCA TTTTCTCAAC TTGGAGGGAG ATAAGTGGCT CTGAGCTGTG CTTTCCAAGT 
GGGCAGAAAA TAAAATCTGC TTATCTTCCC CAAAGGCAAA TTCACATACC AGCTGTTTTT 
CAGTCTCCTG CTCATTATAA GCAGACTTTC ACATCTTGCC TCATAGAACA TCTAAATATA 
TTGCTGTTTG GGTTAGCACA AAACCTGCAG AAAGCTCTTT CAAAAGTTGA CATATCATTT 
TATACATCAT TGAAGGGAGA GAAACTGAAA AACGCAGAAA ATAATGTACC ATCCTGCCAT 
CATAGTCAAC CTGCAAAACT TGTCATGGTT AAAAAGGAAG GTCCAAATAA GGGTCGTCTC 
TTTTATACAT GTGATGGACC CAAAGCTGAT CGATGTAAAT TCTTTAAATG GCTTGAGGAC 
GTGACTCCAG GATATTCAAC ACAGGAAGGA GCTCGACCTG GCATGGTTTT AAGTGATATT 
AAGAGTATTG GCTTATATTT AAGAAGTCAA AAGATACCAC TTTATGAGGA ATGCCAGCTT 
TTGGTGAGAA AAGGATTTGA TTTTCAGAGA AAACAGTATG GCAAACTAAA GAAGTTTACT 
ACTGTAAATC CTGAGTTTTA TAATGAACCA AAAACCAAAC TTTATCTTAA GCTAAGTCGG 
AAGGAAAGAT CTTCAGCTTA TAGCAAAAAT GATCTTTGGG TGGTTTCAAA AACCCTAGAC 
TTTGAGCTGG ATACTTTTAT CGCATGTAGT GCTTTCTTTG GACCATCATC TATCAATGAG 
ATAGAAATAC TGCCTTTGAA AGGCTATTTC CCTTCTAATT GGCCCACTAA CATGGTTGTC 
CATGCGTTAT TGGTTTGTAA TGCTAGCACA GAACTGACTA CTTTGAAAAA CATTCAGGAC 
TACTTTAATC CAGCTACTCT ACCTCTAACA CAGTACCTGT TAACAACGTC TTCGCCAACT 
ATAGTTAGTA ACAAAAGAGT CAGTAAGAGA AAATTTATCC CACCAGCCTT CACAAATGTC 
AGTACAAAAT TTGAACTACT CAGCCTAGGA GCAACATTGA AGTTAGCTAG TGAGTTGATT 
CAGGTACACA AGTTAAACAA GGATCAAGCT ACAGCTCTAA TTCAAATAGC TCAAATGATG 
GCATCACATG AAAGCATTGA AGAAGTGAAG GAACTGCAAA CTCATACCTT CCCTATCACA 
ATCATACATG GTGTGTTTGG AGCAGGAAAG AGTTACTTGC TGGCAGTGGT GATTTTGTTC 
TTTGTACAGC TGTTTGAAAA GAGTGAAGCT CCCACCATTG GAAATGCAAG GCCGTGGAAA 
CTTCTGATTT CTTCTTCTAC TAATGTGGCT GTTGACAGAG TACTTCTTGG GCTTCTCAGT 
CTTGGATTTG AAAACTTTAT CAGAGTTGGG AGTGTTAGGA AGATTGCCAA ACCAATTTTA 
CCTTATAGCT TGCATGCTGG CTCAGAAAAT GAAAGTGAAC AGTTAAAAGA ACTACATGCA 
CTAATGAAAG AAGACCTGAC TCCTACGGAA AGAGTCTATG TGAGAAAAAG CATTGAGCAG 
CATAAACTGG GGACCAATAG AACCCTGCTG AAGCAGGTTC GAGTAGTTGG AGTTACCTGT 
GCAGCCTGCC CATTCCCATG CATGAATGAT CTTAAATTTC CTGTAGTTGT GCTGGATGAG 
TGTAGTCAGA TAACTGAACC GGCCTCTCTC CTTCCCATTG CAAGGTTTGA GTGTGAAAAG 
CTGATTCTTG TTGGGGATCC CAAACAGCTA CCTCCTACTA TTCAGGGTTC TGATGCAGCT 
CATGAAAATG GATTGGAACA AACTCTTTTT GATCGACTTT GCTTAATGGG TCACAAGCCA 
ATTCTATTGA GAACTCAATA CCGTTGTCAT CCTGCAATCA GTGCTATTGC TAATGATCTG 
TTTTACAAAG GAGCCCTCAT GAATGGTGTA ACAGAAATAG AGCGGAGCCC TTTATTGGAA 
TGGCTACCAA CCCTGTGTTT TTATAATGTT AAAGGACTAG AACAGATAGA AAGAGATAAC 
AGCTTTCATA ATGTGGCAGA AGCTACGTTT ACACTCAAGC TGATTCAATC ACTGATTGCA 
AGTGGAATAG CAGGCTCTAT GATTGGTGTG ATAACATTAT ACAAATCCCA GATGTACAAG 
CTTTGTCATT TACTCAGTGC TGTGGACTTT CACCATCCTG ATATTAAAAC TGTGCAGGTG 
TCCACAGTAG ATGCTTTTCA GGGAGCTGAA AAGGAGATCA TTATTCTGTC CTGTGTAAGG 
ACAAGACAAG TAGGATTCAT TGATTCAGAA AAAAGAATGA ATGTTGCATT GACTAGAGGA 
AAGAGGCATT TGTTGATTGT GGGAAATTTA GCCTGTTTGA GGAAAAATCA ACTTTGGGGA 
CGAGTGATCC AACACTGCGA AGGAAGGGAA GATGGATTGC AACATGCAAA CCAGTATGAA 
CCACAGCTGA ACCATCTCCT TAAAGATTAT TTTGAAAAAC AAGTGGAAGA AAAACAGAAG 
AAAAAGAGTG AAAAAGAGAA ATCTAAAGAT AAATCTCATT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_017008373.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863862.1 Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OHu95282
Clone ID Related Accession (Same CDS sequence)	XM_024454130.1 , XM_017008373.1 , XM_017008372.1 , XM_024454129.1
Accession Version	XM_017008373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein ZGRF1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTTCA TTTTCTCAAC TTGGAGGGAG ATAAGTGGCT CTGAGCTGTG CTTTCCAAGT 
GGGCAGAAAA TAAAATCTGC TTATCTTCCC CAAAGGCAAA TTCACATACC AGCTGTTTTT 
CAGTCTCCTG CTCATTATAA GCAGACTTTC ACATCTTGCC TCATAGAACA TCTAAATATA 
TTGCTGTTTG GGTTAGCACA AAACCTGCAG AAAGCTCTTT CAAAAGTTGA CATATCATTT 
TATACATCAT TGAAGGGAGA GAAACTGAAA AACGCAGAAA ATAATGTACC ATCCTGCCAT 
CATAGTCAAC CTGCAAAACT TGTCATGGTT AAAAAGGAAG GTCCAAATAA GGGTCGTCTC 
TTTTATACAT GTGATGGACC CAAAGCTGAT CGATGTAAAT TCTTTAAATG GCTTGAGGAC 
GTGACTCCAG GATATTCAAC ACAGGAAGGA GCTCGACCTG GCATGGTTTT AAGTGATATT 
AAGAGTATTG GCTTATATTT AAGAAGTCAA AAGATACCAC TTTATGAGGA ATGCCAGCTT 
TTGGTGAGAA AAGGATTTGA TTTTCAGAGA AAACAGTATG GCAAACTAAA GAAGTTTACT 
ACTGTAAATC CTGAGTTTTA TAATGAACCA AAAACCAAAC TTTATCTTAA GCTAAGTCGG 
AAGGAAAGAT CTTCAGCTTA TAGCAAAAAT GATCTTTGGG TGGTTTCAAA AACCCTAGAC 
TTTGAGCTGG ATACTTTTAT CGCATGTAGT GCTTTCTTTG GACCATCATC TATCAATGAG 
ATAGAAATAC TGCCTTTGAA AGGCTATTTC CCTTCTAATT GGCCCACTAA CATGGTTGTC 
CATGCGTTAT TGGTTTGTAA TGCTAGCACA GAACTGACTA CTTTGAAAAA CATTCAGGAC 
TACTTTAATC CAGCTACTCT ACCTCTAACA CAGTACCTGT TAACAACGTC TTCGCCAACT 
ATAGTTAGTA ACAAAAGAGT CAGTAAGAGA AAATTTATCC CACCAGCCTT CACAAATGTC 
AGTACAAAAT TTGAACTACT CAGCCTAGGA GCAACATTGA AGTTAGCTAG TGAGTTGATT 
CAGGTACACA AGTTAAACAA GGATCAAGCT ACAGCTCTAA TTCAAATAGC TCAAATGATG 
GCATCACATG AAAGCATTGA AGAAGTGAAG GAACTGCAAA CTCATACCTT CCCTATCACA 
ATCATACATG GTGTGTTTGG AGCAGGAAAG AGTTACTTGC TGGCAGTGGT GATTTTGTTC 
TTTGTACAGC TGTTTGAAAA GAGTGAAGCT CCCACCATTG GAAATGCAAG GCCGTGGAAA 
CTTCTGATTT CTTCTTCTAC TAATGTGGCT GTTGACAGAG TACTTCTTGG GCTTCTCAGT 
CTTGGATTTG AAAACTTTAT CAGAGTTGGG AGTGTTAGGA AGATTGCCAA ACCAATTTTA 
CCTTATAGCT TGCATGCTGG CTCAGAAAAT GAAAGTGAAC AGTTAAAAGA ACTACATGCA 
CTAATGAAAG AAGACCTGAC TCCTACGGAA AGAGTCTATG TGAGAAAAAG CATTGAGCAG 
CATAAACTGG GGACCAATAG AACCCTGCTG AAGCAGGTTC GAGTAGTTGG AGTTACCTGT 
GCAGCCTGCC CATTCCCATG CATGAATGAT CTTAAATTTC CTGTAGTTGT GCTGGATGAG 
TGTAGTCAGA TAACTGAACC GGCCTCTCTC CTTCCCATTG CAAGGTTTGA GTGTGAAAAG 
CTGATTCTTG TTGGGGATCC CAAACAGCTA CCTCCTACTA TTCAGGGTTC TGATGCAGCT 
CATGAAAATG GATTGGAACA AACTCTTTTT GATCGACTTT GCTTAATGGG TCACAAGCCA 
ATTCTATTGA GAACTCAATA CCGTTGTCAT CCTGCAATCA GTGCTATTGC TAATGATCTG 
TTTTACAAAG GAGCCCTCAT GAATGGTGTA ACAGAAATAG AGCGGAGCCC TTTATTGGAA 
TGGCTACCAA CCCTGTGTTT TTATAATGTT AAAGGACTAG AACAGATAGA AAGAGATAAC 
AGCTTTCATA ATGTGGCAGA AGCTACGTTT ACACTCAAGC TGATTCAATC ACTGATTGCA 
AGTGGAATAG CAGGCTCTAT GATTGGTGTG ATAACATTAT ACAAATCCCA GATGTACAAG 
CTTTGTCATT TACTCAGTGC TGTGGACTTT CACCATCCTG ATATTAAAAC TGTGCAGGTG 
TCCACAGTAG ATGCTTTTCA GGGAGCTGAA AAGGAGATCA TTATTCTGTC CTGTGTAAGG 
ACAAGACAAG TAGGATTCAT TGATTCAGAA AAAAGAATGA ATGTTGCATT GACTAGAGGA 
AAGAGGCATT TGTTGATTGT GGGAAATTTA GCCTGTTTGA GGAAAAATCA ACTTTGGGGA 
CGAGTGATCC AACACTGCGA AGGAAGGGAA GATGGATTGC AACATGCAAA CCAGTATGAA 
CCACAGCTGA ACCATCTCCT TAAAGATTAT TTTGAAAAAC AAGTGGAAGA AAAACAGAAG 
AAAAAGAGTG AAAAAGAGAA ATCTAAAGAT AAATCTCATT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863862.1
CDS	2933..5497
Translation	MIFIFSTWREISGSELCFPSGQKIKSAYLPQRQIHIPAVFQSPAHYKQTFTSCLIEHLNILLFGLAQNLQKALSKVDISFYTSLKGEKLKNAENNVPSCHHSQPAKLVMVKKEGPNKGRLFYTCDGPKADRCKFFKWLEDVTPGYSTQEGARPGMVLSDIKSIGLYLRSQKIPLYEECQLLVRKGFDFQRKQYGKLKKFTTVNPEFYNEPKTKLYLKLSRKERSSAYSKNDLWVVSKTLDFELDTFIACSAFFGPSSINEIEILPLKGYFPSNWPTNMVVHALLVCNASTELTTLKNIQDYFNPATLPLTQYLLTTSSPTIVSNKRVSKRKFIPPAFTNVSTKFELLSLGATLKLASELIQVHKLNKDQATALIQIAQMMASHESIEEVKELQTHTFPITIIHGVFGAGKSYLLAVVILFFVQLFEKSEAPTIGNARPWKLLISSSTNVAVDRVLLGLLSLGFENFIRVGSVRKIAKPILPYSLHAGSENESEQLKELHALMKEDLTPTERVYVRKSIEQHKLGTNRTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPAISAIANDLFYKGALMNGVTEIERSPLLEWLPTLCFYNVKGLEQIERDNSFHNVAEATFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAVDFHHPDIKTVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGKRHLLIVGNLACLRKNQLWGRVIQHCEGREDGLQHANQYEPQLNHLLKDYFEKQVEEKQKKKSEKEKSKDKSHS

Target ORF information:

RefSeq Version	XM_017008373.1
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008373.1

ORF Insert Sequence:

ATGATCTTCA TTTTCTCAAC TTGGAGGGAG ATAAGTGGCT CTGAGCTGTG CTTTCCAAGT 
GGGCAGAAAA TAAAATCTGC TTATCTTCCC CAAAGGCAAA TTCACATACC AGCTGTTTTT 
CAGTCTCCTG CTCATTATAA GCAGACTTTC ACATCTTGCC TCATAGAACA TCTAAATATA 
TTGCTGTTTG GGTTAGCACA AAACCTGCAG AAAGCTCTTT CAAAAGTTGA CATATCATTT 
TATACATCAT TGAAGGGAGA GAAACTGAAA AACGCAGAAA ATAATGTACC ATCCTGCCAT 
CATAGTCAAC CTGCAAAACT TGTCATGGTT AAAAAGGAAG GTCCAAATAA GGGTCGTCTC 
TTTTATACAT GTGATGGACC CAAAGCTGAT CGATGTAAAT TCTTTAAATG GCTTGAGGAC 
GTGACTCCAG GATATTCAAC ACAGGAAGGA GCTCGACCTG GCATGGTTTT AAGTGATATT 
AAGAGTATTG GCTTATATTT AAGAAGTCAA AAGATACCAC TTTATGAGGA ATGCCAGCTT 
TTGGTGAGAA AAGGATTTGA TTTTCAGAGA AAACAGTATG GCAAACTAAA GAAGTTTACT 
ACTGTAAATC CTGAGTTTTA TAATGAACCA AAAACCAAAC TTTATCTTAA GCTAAGTCGG 
AAGGAAAGAT CTTCAGCTTA TAGCAAAAAT GATCTTTGGG TGGTTTCAAA AACCCTAGAC 
TTTGAGCTGG ATACTTTTAT CGCATGTAGT GCTTTCTTTG GACCATCATC TATCAATGAG 
ATAGAAATAC TGCCTTTGAA AGGCTATTTC CCTTCTAATT GGCCCACTAA CATGGTTGTC 
CATGCGTTAT TGGTTTGTAA TGCTAGCACA GAACTGACTA CTTTGAAAAA CATTCAGGAC 
TACTTTAATC CAGCTACTCT ACCTCTAACA CAGTACCTGT TAACAACGTC TTCGCCAACT 
ATAGTTAGTA ACAAAAGAGT CAGTAAGAGA AAATTTATCC CACCAGCCTT CACAAATGTC 
AGTACAAAAT TTGAACTACT CAGCCTAGGA GCAACATTGA AGTTAGCTAG TGAGTTGATT 
CAGGTACACA AGTTAAACAA GGATCAAGCT ACAGCTCTAA TTCAAATAGC TCAAATGATG 
GCATCACATG AAAGCATTGA AGAAGTGAAG GAACTGCAAA CTCATACCTT CCCTATCACA 
ATCATACATG GTGTGTTTGG AGCAGGAAAG AGTTACTTGC TGGCAGTGGT GATTTTGTTC 
TTTGTACAGC TGTTTGAAAA GAGTGAAGCT CCCACCATTG GAAATGCAAG GCCGTGGAAA 
CTTCTGATTT CTTCTTCTAC TAATGTGGCT GTTGACAGAG TACTTCTTGG GCTTCTCAGT 
CTTGGATTTG AAAACTTTAT CAGAGTTGGG AGTGTTAGGA AGATTGCCAA ACCAATTTTA 
CCTTATAGCT TGCATGCTGG CTCAGAAAAT GAAAGTGAAC AGTTAAAAGA ACTACATGCA 
CTAATGAAAG AAGACCTGAC TCCTACGGAA AGAGTCTATG TGAGAAAAAG CATTGAGCAG 
CATAAACTGG GGACCAATAG AACCCTGCTG AAGCAGGTTC GAGTAGTTGG AGTTACCTGT 
GCAGCCTGCC CATTCCCATG CATGAATGAT CTTAAATTTC CTGTAGTTGT GCTGGATGAG 
TGTAGTCAGA TAACTGAACC GGCCTCTCTC CTTCCCATTG CAAGGTTTGA GTGTGAAAAG 
CTGATTCTTG TTGGGGATCC CAAACAGCTA CCTCCTACTA TTCAGGGTTC TGATGCAGCT 
CATGAAAATG GATTGGAACA AACTCTTTTT GATCGACTTT GCTTAATGGG TCACAAGCCA 
ATTCTATTGA GAACTCAATA CCGTTGTCAT CCTGCAATCA GTGCTATTGC TAATGATCTG 
TTTTACAAAG GAGCCCTCAT GAATGGTGTA ACAGAAATAG AGCGGAGCCC TTTATTGGAA 
TGGCTACCAA CCCTGTGTTT TTATAATGTT AAAGGACTAG AACAGATAGA AAGAGATAAC 
AGCTTTCATA ATGTGGCAGA AGCTACGTTT ACACTCAAGC TGATTCAATC ACTGATTGCA 
AGTGGAATAG CAGGCTCTAT GATTGGTGTG ATAACATTAT ACAAATCCCA GATGTACAAG 
CTTTGTCATT TACTCAGTGC TGTGGACTTT CACCATCCTG ATATTAAAAC TGTGCAGGTG 
TCCACAGTAG ATGCTTTTCA GGGAGCTGAA AAGGAGATCA TTATTCTGTC CTGTGTAAGG 
ACAAGACAAG TAGGATTCAT TGATTCAGAA AAAAGAATGA ATGTTGCATT GACTAGAGGA 
AAGAGGCATT TGTTGATTGT GGGAAATTTA GCCTGTTTGA GGAAAAATCA ACTTTGGGGA 
CGAGTGATCC AACACTGCGA AGGAAGGGAA GATGGATTGC AACATGCAAA CCAGTATGAA 
CCACAGCTGA ACCATCTCCT TAAAGATTAT TTTGAAAAAC AAGTGGAAGA AAAACAGAAG 
AAAAAGAGTG AAAAAGAGAA ATCTAAAGAT AAATCTCATT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_017008372.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863861.1 Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OHu95282
Clone ID Related Accession (Same CDS sequence)	XM_024454130.1 , XM_017008373.1 , XM_017008372.1 , XM_024454129.1
Accession Version	XM_017008372.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein ZGRF1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTTCA TTTTCTCAAC TTGGAGGGAG ATAAGTGGCT CTGAGCTGTG CTTTCCAAGT 
GGGCAGAAAA TAAAATCTGC TTATCTTCCC CAAAGGCAAA TTCACATACC AGCTGTTTTT 
CAGTCTCCTG CTCATTATAA GCAGACTTTC ACATCTTGCC TCATAGAACA TCTAAATATA 
TTGCTGTTTG GGTTAGCACA AAACCTGCAG AAAGCTCTTT CAAAAGTTGA CATATCATTT 
TATACATCAT TGAAGGGAGA GAAACTGAAA AACGCAGAAA ATAATGTACC ATCCTGCCAT 
CATAGTCAAC CTGCAAAACT TGTCATGGTT AAAAAGGAAG GTCCAAATAA GGGTCGTCTC 
TTTTATACAT GTGATGGACC CAAAGCTGAT CGATGTAAAT TCTTTAAATG GCTTGAGGAC 
GTGACTCCAG GATATTCAAC ACAGGAAGGA GCTCGACCTG GCATGGTTTT AAGTGATATT 
AAGAGTATTG GCTTATATTT AAGAAGTCAA AAGATACCAC TTTATGAGGA ATGCCAGCTT 
TTGGTGAGAA AAGGATTTGA TTTTCAGAGA AAACAGTATG GCAAACTAAA GAAGTTTACT 
ACTGTAAATC CTGAGTTTTA TAATGAACCA AAAACCAAAC TTTATCTTAA GCTAAGTCGG 
AAGGAAAGAT CTTCAGCTTA TAGCAAAAAT GATCTTTGGG TGGTTTCAAA AACCCTAGAC 
TTTGAGCTGG ATACTTTTAT CGCATGTAGT GCTTTCTTTG GACCATCATC TATCAATGAG 
ATAGAAATAC TGCCTTTGAA AGGCTATTTC CCTTCTAATT GGCCCACTAA CATGGTTGTC 
CATGCGTTAT TGGTTTGTAA TGCTAGCACA GAACTGACTA CTTTGAAAAA CATTCAGGAC 
TACTTTAATC CAGCTACTCT ACCTCTAACA CAGTACCTGT TAACAACGTC TTCGCCAACT 
ATAGTTAGTA ACAAAAGAGT CAGTAAGAGA AAATTTATCC CACCAGCCTT CACAAATGTC 
AGTACAAAAT TTGAACTACT CAGCCTAGGA GCAACATTGA AGTTAGCTAG TGAGTTGATT 
CAGGTACACA AGTTAAACAA GGATCAAGCT ACAGCTCTAA TTCAAATAGC TCAAATGATG 
GCATCACATG AAAGCATTGA AGAAGTGAAG GAACTGCAAA CTCATACCTT CCCTATCACA 
ATCATACATG GTGTGTTTGG AGCAGGAAAG AGTTACTTGC TGGCAGTGGT GATTTTGTTC 
TTTGTACAGC TGTTTGAAAA GAGTGAAGCT CCCACCATTG GAAATGCAAG GCCGTGGAAA 
CTTCTGATTT CTTCTTCTAC TAATGTGGCT GTTGACAGAG TACTTCTTGG GCTTCTCAGT 
CTTGGATTTG AAAACTTTAT CAGAGTTGGG AGTGTTAGGA AGATTGCCAA ACCAATTTTA 
CCTTATAGCT TGCATGCTGG CTCAGAAAAT GAAAGTGAAC AGTTAAAAGA ACTACATGCA 
CTAATGAAAG AAGACCTGAC TCCTACGGAA AGAGTCTATG TGAGAAAAAG CATTGAGCAG 
CATAAACTGG GGACCAATAG AACCCTGCTG AAGCAGGTTC GAGTAGTTGG AGTTACCTGT 
GCAGCCTGCC CATTCCCATG CATGAATGAT CTTAAATTTC CTGTAGTTGT GCTGGATGAG 
TGTAGTCAGA TAACTGAACC GGCCTCTCTC CTTCCCATTG CAAGGTTTGA GTGTGAAAAG 
CTGATTCTTG TTGGGGATCC CAAACAGCTA CCTCCTACTA TTCAGGGTTC TGATGCAGCT 
CATGAAAATG GATTGGAACA AACTCTTTTT GATCGACTTT GCTTAATGGG TCACAAGCCA 
ATTCTATTGA GAACTCAATA CCGTTGTCAT CCTGCAATCA GTGCTATTGC TAATGATCTG 
TTTTACAAAG GAGCCCTCAT GAATGGTGTA ACAGAAATAG AGCGGAGCCC TTTATTGGAA 
TGGCTACCAA CCCTGTGTTT TTATAATGTT AAAGGACTAG AACAGATAGA AAGAGATAAC 
AGCTTTCATA ATGTGGCAGA AGCTACGTTT ACACTCAAGC TGATTCAATC ACTGATTGCA 
AGTGGAATAG CAGGCTCTAT GATTGGTGTG ATAACATTAT ACAAATCCCA GATGTACAAG 
CTTTGTCATT TACTCAGTGC TGTGGACTTT CACCATCCTG ATATTAAAAC TGTGCAGGTG 
TCCACAGTAG ATGCTTTTCA GGGAGCTGAA AAGGAGATCA TTATTCTGTC CTGTGTAAGG 
ACAAGACAAG TAGGATTCAT TGATTCAGAA AAAAGAATGA ATGTTGCATT GACTAGAGGA 
AAGAGGCATT TGTTGATTGT GGGAAATTTA GCCTGTTTGA GGAAAAATCA ACTTTGGGGA 
CGAGTGATCC AACACTGCGA AGGAAGGGAA GATGGATTGC AACATGCAAA CCAGTATGAA 
CCACAGCTGA ACCATCTCCT TAAAGATTAT TTTGAAAAAC AAGTGGAAGA AAAACAGAAG 
AAAAAGAGTG AAAAAGAGAA ATCTAAAGAT AAATCTCATT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863861.1
CDS	2996..5560
Translation	MIFIFSTWREISGSELCFPSGQKIKSAYLPQRQIHIPAVFQSPAHYKQTFTSCLIEHLNILLFGLAQNLQKALSKVDISFYTSLKGEKLKNAENNVPSCHHSQPAKLVMVKKEGPNKGRLFYTCDGPKADRCKFFKWLEDVTPGYSTQEGARPGMVLSDIKSIGLYLRSQKIPLYEECQLLVRKGFDFQRKQYGKLKKFTTVNPEFYNEPKTKLYLKLSRKERSSAYSKNDLWVVSKTLDFELDTFIACSAFFGPSSINEIEILPLKGYFPSNWPTNMVVHALLVCNASTELTTLKNIQDYFNPATLPLTQYLLTTSSPTIVSNKRVSKRKFIPPAFTNVSTKFELLSLGATLKLASELIQVHKLNKDQATALIQIAQMMASHESIEEVKELQTHTFPITIIHGVFGAGKSYLLAVVILFFVQLFEKSEAPTIGNARPWKLLISSSTNVAVDRVLLGLLSLGFENFIRVGSVRKIAKPILPYSLHAGSENESEQLKELHALMKEDLTPTERVYVRKSIEQHKLGTNRTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPAISAIANDLFYKGALMNGVTEIERSPLLEWLPTLCFYNVKGLEQIERDNSFHNVAEATFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAVDFHHPDIKTVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGKRHLLIVGNLACLRKNQLWGRVIQHCEGREDGLQHANQYEPQLNHLLKDYFEKQVEEKQKKKSEKEKSKDKSHS

Target ORF information:

RefSeq Version	XM_017008372.1
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008372.1

ORF Insert Sequence:

ATGATCTTCA TTTTCTCAAC TTGGAGGGAG ATAAGTGGCT CTGAGCTGTG CTTTCCAAGT 
GGGCAGAAAA TAAAATCTGC TTATCTTCCC CAAAGGCAAA TTCACATACC AGCTGTTTTT 
CAGTCTCCTG CTCATTATAA GCAGACTTTC ACATCTTGCC TCATAGAACA TCTAAATATA 
TTGCTGTTTG GGTTAGCACA AAACCTGCAG AAAGCTCTTT CAAAAGTTGA CATATCATTT 
TATACATCAT TGAAGGGAGA GAAACTGAAA AACGCAGAAA ATAATGTACC ATCCTGCCAT 
CATAGTCAAC CTGCAAAACT TGTCATGGTT AAAAAGGAAG GTCCAAATAA GGGTCGTCTC 
TTTTATACAT GTGATGGACC CAAAGCTGAT CGATGTAAAT TCTTTAAATG GCTTGAGGAC 
GTGACTCCAG GATATTCAAC ACAGGAAGGA GCTCGACCTG GCATGGTTTT AAGTGATATT 
AAGAGTATTG GCTTATATTT AAGAAGTCAA AAGATACCAC TTTATGAGGA ATGCCAGCTT 
TTGGTGAGAA AAGGATTTGA TTTTCAGAGA AAACAGTATG GCAAACTAAA GAAGTTTACT 
ACTGTAAATC CTGAGTTTTA TAATGAACCA AAAACCAAAC TTTATCTTAA GCTAAGTCGG 
AAGGAAAGAT CTTCAGCTTA TAGCAAAAAT GATCTTTGGG TGGTTTCAAA AACCCTAGAC 
TTTGAGCTGG ATACTTTTAT CGCATGTAGT GCTTTCTTTG GACCATCATC TATCAATGAG 
ATAGAAATAC TGCCTTTGAA AGGCTATTTC CCTTCTAATT GGCCCACTAA CATGGTTGTC 
CATGCGTTAT TGGTTTGTAA TGCTAGCACA GAACTGACTA CTTTGAAAAA CATTCAGGAC 
TACTTTAATC CAGCTACTCT ACCTCTAACA CAGTACCTGT TAACAACGTC TTCGCCAACT 
ATAGTTAGTA ACAAAAGAGT CAGTAAGAGA AAATTTATCC CACCAGCCTT CACAAATGTC 
AGTACAAAAT TTGAACTACT CAGCCTAGGA GCAACATTGA AGTTAGCTAG TGAGTTGATT 
CAGGTACACA AGTTAAACAA GGATCAAGCT ACAGCTCTAA TTCAAATAGC TCAAATGATG 
GCATCACATG AAAGCATTGA AGAAGTGAAG GAACTGCAAA CTCATACCTT CCCTATCACA 
ATCATACATG GTGTGTTTGG AGCAGGAAAG AGTTACTTGC TGGCAGTGGT GATTTTGTTC 
TTTGTACAGC TGTTTGAAAA GAGTGAAGCT CCCACCATTG GAAATGCAAG GCCGTGGAAA 
CTTCTGATTT CTTCTTCTAC TAATGTGGCT GTTGACAGAG TACTTCTTGG GCTTCTCAGT 
CTTGGATTTG AAAACTTTAT CAGAGTTGGG AGTGTTAGGA AGATTGCCAA ACCAATTTTA 
CCTTATAGCT TGCATGCTGG CTCAGAAAAT GAAAGTGAAC AGTTAAAAGA ACTACATGCA 
CTAATGAAAG AAGACCTGAC TCCTACGGAA AGAGTCTATG TGAGAAAAAG CATTGAGCAG 
CATAAACTGG GGACCAATAG AACCCTGCTG AAGCAGGTTC GAGTAGTTGG AGTTACCTGT 
GCAGCCTGCC CATTCCCATG CATGAATGAT CTTAAATTTC CTGTAGTTGT GCTGGATGAG 
TGTAGTCAGA TAACTGAACC GGCCTCTCTC CTTCCCATTG CAAGGTTTGA GTGTGAAAAG 
CTGATTCTTG TTGGGGATCC CAAACAGCTA CCTCCTACTA TTCAGGGTTC TGATGCAGCT 
CATGAAAATG GATTGGAACA AACTCTTTTT GATCGACTTT GCTTAATGGG TCACAAGCCA 
ATTCTATTGA GAACTCAATA CCGTTGTCAT CCTGCAATCA GTGCTATTGC TAATGATCTG 
TTTTACAAAG GAGCCCTCAT GAATGGTGTA ACAGAAATAG AGCGGAGCCC TTTATTGGAA 
TGGCTACCAA CCCTGTGTTT TTATAATGTT AAAGGACTAG AACAGATAGA AAGAGATAAC 
AGCTTTCATA ATGTGGCAGA AGCTACGTTT ACACTCAAGC TGATTCAATC ACTGATTGCA 
AGTGGAATAG CAGGCTCTAT GATTGGTGTG ATAACATTAT ACAAATCCCA GATGTACAAG 
CTTTGTCATT TACTCAGTGC TGTGGACTTT CACCATCCTG ATATTAAAAC TGTGCAGGTG 
TCCACAGTAG ATGCTTTTCA GGGAGCTGAA AAGGAGATCA TTATTCTGTC CTGTGTAAGG 
ACAAGACAAG TAGGATTCAT TGATTCAGAA AAAAGAATGA ATGTTGCATT GACTAGAGGA 
AAGAGGCATT TGTTGATTGT GGGAAATTTA GCCTGTTTGA GGAAAAATCA ACTTTGGGGA 
CGAGTGATCC AACACTGCGA AGGAAGGGAA GATGGATTGC AACATGCAAA CCAGTATGAA 
CCACAGCTGA ACCATCTCCT TAAAGATTAT TTTGAAAAAC AAGTGGAAGA AAAACAGAAG 
AAAAAGAGTG AAAAAGAGAA ATCTAAAGAT AAATCTCATT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_024454129.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309897.1 Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OHu95282
Clone ID Related Accession (Same CDS sequence)	XM_024454130.1 , XM_017008373.1 , XM_017008372.1 , XM_024454129.1
Accession Version	XM_024454129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein ZGRF1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTTCA TTTTCTCAAC TTGGAGGGAG ATAAGTGGCT CTGAGCTGTG CTTTCCAAGT 
GGGCAGAAAA TAAAATCTGC TTATCTTCCC CAAAGGCAAA TTCACATACC AGCTGTTTTT 
CAGTCTCCTG CTCATTATAA GCAGACTTTC ACATCTTGCC TCATAGAACA TCTAAATATA 
TTGCTGTTTG GGTTAGCACA AAACCTGCAG AAAGCTCTTT CAAAAGTTGA CATATCATTT 
TATACATCAT TGAAGGGAGA GAAACTGAAA AACGCAGAAA ATAATGTACC ATCCTGCCAT 
CATAGTCAAC CTGCAAAACT TGTCATGGTT AAAAAGGAAG GTCCAAATAA GGGTCGTCTC 
TTTTATACAT GTGATGGACC CAAAGCTGAT CGATGTAAAT TCTTTAAATG GCTTGAGGAC 
GTGACTCCAG GATATTCAAC ACAGGAAGGA GCTCGACCTG GCATGGTTTT AAGTGATATT 
AAGAGTATTG GCTTATATTT AAGAAGTCAA AAGATACCAC TTTATGAGGA ATGCCAGCTT 
TTGGTGAGAA AAGGATTTGA TTTTCAGAGA AAACAGTATG GCAAACTAAA GAAGTTTACT 
ACTGTAAATC CTGAGTTTTA TAATGAACCA AAAACCAAAC TTTATCTTAA GCTAAGTCGG 
AAGGAAAGAT CTTCAGCTTA TAGCAAAAAT GATCTTTGGG TGGTTTCAAA AACCCTAGAC 
TTTGAGCTGG ATACTTTTAT CGCATGTAGT GCTTTCTTTG GACCATCATC TATCAATGAG 
ATAGAAATAC TGCCTTTGAA AGGCTATTTC CCTTCTAATT GGCCCACTAA CATGGTTGTC 
CATGCGTTAT TGGTTTGTAA TGCTAGCACA GAACTGACTA CTTTGAAAAA CATTCAGGAC 
TACTTTAATC CAGCTACTCT ACCTCTAACA CAGTACCTGT TAACAACGTC TTCGCCAACT 
ATAGTTAGTA ACAAAAGAGT CAGTAAGAGA AAATTTATCC CACCAGCCTT CACAAATGTC 
AGTACAAAAT TTGAACTACT CAGCCTAGGA GCAACATTGA AGTTAGCTAG TGAGTTGATT 
CAGGTACACA AGTTAAACAA GGATCAAGCT ACAGCTCTAA TTCAAATAGC TCAAATGATG 
GCATCACATG AAAGCATTGA AGAAGTGAAG GAACTGCAAA CTCATACCTT CCCTATCACA 
ATCATACATG GTGTGTTTGG AGCAGGAAAG AGTTACTTGC TGGCAGTGGT GATTTTGTTC 
TTTGTACAGC TGTTTGAAAA GAGTGAAGCT CCCACCATTG GAAATGCAAG GCCGTGGAAA 
CTTCTGATTT CTTCTTCTAC TAATGTGGCT GTTGACAGAG TACTTCTTGG GCTTCTCAGT 
CTTGGATTTG AAAACTTTAT CAGAGTTGGG AGTGTTAGGA AGATTGCCAA ACCAATTTTA 
CCTTATAGCT TGCATGCTGG CTCAGAAAAT GAAAGTGAAC AGTTAAAAGA ACTACATGCA 
CTAATGAAAG AAGACCTGAC TCCTACGGAA AGAGTCTATG TGAGAAAAAG CATTGAGCAG 
CATAAACTGG GGACCAATAG AACCCTGCTG AAGCAGGTTC GAGTAGTTGG AGTTACCTGT 
GCAGCCTGCC CATTCCCATG CATGAATGAT CTTAAATTTC CTGTAGTTGT GCTGGATGAG 
TGTAGTCAGA TAACTGAACC GGCCTCTCTC CTTCCCATTG CAAGGTTTGA GTGTGAAAAG 
CTGATTCTTG TTGGGGATCC CAAACAGCTA CCTCCTACTA TTCAGGGTTC TGATGCAGCT 
CATGAAAATG GATTGGAACA AACTCTTTTT GATCGACTTT GCTTAATGGG TCACAAGCCA 
ATTCTATTGA GAACTCAATA CCGTTGTCAT CCTGCAATCA GTGCTATTGC TAATGATCTG 
TTTTACAAAG GAGCCCTCAT GAATGGTGTA ACAGAAATAG AGCGGAGCCC TTTATTGGAA 
TGGCTACCAA CCCTGTGTTT TTATAATGTT AAAGGACTAG AACAGATAGA AAGAGATAAC 
AGCTTTCATA ATGTGGCAGA AGCTACGTTT ACACTCAAGC TGATTCAATC ACTGATTGCA 
AGTGGAATAG CAGGCTCTAT GATTGGTGTG ATAACATTAT ACAAATCCCA GATGTACAAG 
CTTTGTCATT TACTCAGTGC TGTGGACTTT CACCATCCTG ATATTAAAAC TGTGCAGGTG 
TCCACAGTAG ATGCTTTTCA GGGAGCTGAA AAGGAGATCA TTATTCTGTC CTGTGTAAGG 
ACAAGACAAG TAGGATTCAT TGATTCAGAA AAAAGAATGA ATGTTGCATT GACTAGAGGA 
AAGAGGCATT TGTTGATTGT GGGAAATTTA GCCTGTTTGA GGAAAAATCA ACTTTGGGGA 
CGAGTGATCC AACACTGCGA AGGAAGGGAA GATGGATTGC AACATGCAAA CCAGTATGAA 
CCACAGCTGA ACCATCTCCT TAAAGATTAT TTTGAAAAAC AAGTGGAAGA AAAACAGAAG 
AAAAAGAGTG AAAAAGAGAA ATCTAAAGAT AAATCTCATT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309897.1
CDS	497..3061
Translation	MIFIFSTWREISGSELCFPSGQKIKSAYLPQRQIHIPAVFQSPAHYKQTFTSCLIEHLNILLFGLAQNLQKALSKVDISFYTSLKGEKLKNAENNVPSCHHSQPAKLVMVKKEGPNKGRLFYTCDGPKADRCKFFKWLEDVTPGYSTQEGARPGMVLSDIKSIGLYLRSQKIPLYEECQLLVRKGFDFQRKQYGKLKKFTTVNPEFYNEPKTKLYLKLSRKERSSAYSKNDLWVVSKTLDFELDTFIACSAFFGPSSINEIEILPLKGYFPSNWPTNMVVHALLVCNASTELTTLKNIQDYFNPATLPLTQYLLTTSSPTIVSNKRVSKRKFIPPAFTNVSTKFELLSLGATLKLASELIQVHKLNKDQATALIQIAQMMASHESIEEVKELQTHTFPITIIHGVFGAGKSYLLAVVILFFVQLFEKSEAPTIGNARPWKLLISSSTNVAVDRVLLGLLSLGFENFIRVGSVRKIAKPILPYSLHAGSENESEQLKELHALMKEDLTPTERVYVRKSIEQHKLGTNRTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPAISAIANDLFYKGALMNGVTEIERSPLLEWLPTLCFYNVKGLEQIERDNSFHNVAEATFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAVDFHHPDIKTVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGKRHLLIVGNLACLRKNQLWGRVIQHCEGREDGLQHANQYEPQLNHLLKDYFEKQVEEKQKKKSEKEKSKDKSHS

Target ORF information:

RefSeq Version	XM_024454129.1
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024454129.1

ORF Insert Sequence:

ATGATCTTCA TTTTCTCAAC TTGGAGGGAG ATAAGTGGCT CTGAGCTGTG CTTTCCAAGT 
GGGCAGAAAA TAAAATCTGC TTATCTTCCC CAAAGGCAAA TTCACATACC AGCTGTTTTT 
CAGTCTCCTG CTCATTATAA GCAGACTTTC ACATCTTGCC TCATAGAACA TCTAAATATA 
TTGCTGTTTG GGTTAGCACA AAACCTGCAG AAAGCTCTTT CAAAAGTTGA CATATCATTT 
TATACATCAT TGAAGGGAGA GAAACTGAAA AACGCAGAAA ATAATGTACC ATCCTGCCAT 
CATAGTCAAC CTGCAAAACT TGTCATGGTT AAAAAGGAAG GTCCAAATAA GGGTCGTCTC 
TTTTATACAT GTGATGGACC CAAAGCTGAT CGATGTAAAT TCTTTAAATG GCTTGAGGAC 
GTGACTCCAG GATATTCAAC ACAGGAAGGA GCTCGACCTG GCATGGTTTT AAGTGATATT 
AAGAGTATTG GCTTATATTT AAGAAGTCAA AAGATACCAC TTTATGAGGA ATGCCAGCTT 
TTGGTGAGAA AAGGATTTGA TTTTCAGAGA AAACAGTATG GCAAACTAAA GAAGTTTACT 
ACTGTAAATC CTGAGTTTTA TAATGAACCA AAAACCAAAC TTTATCTTAA GCTAAGTCGG 
AAGGAAAGAT CTTCAGCTTA TAGCAAAAAT GATCTTTGGG TGGTTTCAAA AACCCTAGAC 
TTTGAGCTGG ATACTTTTAT CGCATGTAGT GCTTTCTTTG GACCATCATC TATCAATGAG 
ATAGAAATAC TGCCTTTGAA AGGCTATTTC CCTTCTAATT GGCCCACTAA CATGGTTGTC 
CATGCGTTAT TGGTTTGTAA TGCTAGCACA GAACTGACTA CTTTGAAAAA CATTCAGGAC 
TACTTTAATC CAGCTACTCT ACCTCTAACA CAGTACCTGT TAACAACGTC TTCGCCAACT 
ATAGTTAGTA ACAAAAGAGT CAGTAAGAGA AAATTTATCC CACCAGCCTT CACAAATGTC 
AGTACAAAAT TTGAACTACT CAGCCTAGGA GCAACATTGA AGTTAGCTAG TGAGTTGATT 
CAGGTACACA AGTTAAACAA GGATCAAGCT ACAGCTCTAA TTCAAATAGC TCAAATGATG 
GCATCACATG AAAGCATTGA AGAAGTGAAG GAACTGCAAA CTCATACCTT CCCTATCACA 
ATCATACATG GTGTGTTTGG AGCAGGAAAG AGTTACTTGC TGGCAGTGGT GATTTTGTTC 
TTTGTACAGC TGTTTGAAAA GAGTGAAGCT CCCACCATTG GAAATGCAAG GCCGTGGAAA 
CTTCTGATTT CTTCTTCTAC TAATGTGGCT GTTGACAGAG TACTTCTTGG GCTTCTCAGT 
CTTGGATTTG AAAACTTTAT CAGAGTTGGG AGTGTTAGGA AGATTGCCAA ACCAATTTTA 
CCTTATAGCT TGCATGCTGG CTCAGAAAAT GAAAGTGAAC AGTTAAAAGA ACTACATGCA 
CTAATGAAAG AAGACCTGAC TCCTACGGAA AGAGTCTATG TGAGAAAAAG CATTGAGCAG 
CATAAACTGG GGACCAATAG AACCCTGCTG AAGCAGGTTC GAGTAGTTGG AGTTACCTGT 
GCAGCCTGCC CATTCCCATG CATGAATGAT CTTAAATTTC CTGTAGTTGT GCTGGATGAG 
TGTAGTCAGA TAACTGAACC GGCCTCTCTC CTTCCCATTG CAAGGTTTGA GTGTGAAAAG 
CTGATTCTTG TTGGGGATCC CAAACAGCTA CCTCCTACTA TTCAGGGTTC TGATGCAGCT 
CATGAAAATG GATTGGAACA AACTCTTTTT GATCGACTTT GCTTAATGGG TCACAAGCCA 
ATTCTATTGA GAACTCAATA CCGTTGTCAT CCTGCAATCA GTGCTATTGC TAATGATCTG 
TTTTACAAAG GAGCCCTCAT GAATGGTGTA ACAGAAATAG AGCGGAGCCC TTTATTGGAA 
TGGCTACCAA CCCTGTGTTT TTATAATGTT AAAGGACTAG AACAGATAGA AAGAGATAAC 
AGCTTTCATA ATGTGGCAGA AGCTACGTTT ACACTCAAGC TGATTCAATC ACTGATTGCA 
AGTGGAATAG CAGGCTCTAT GATTGGTGTG ATAACATTAT ACAAATCCCA GATGTACAAG 
CTTTGTCATT TACTCAGTGC TGTGGACTTT CACCATCCTG ATATTAAAAC TGTGCAGGTG 
TCCACAGTAG ATGCTTTTCA GGGAGCTGAA AAGGAGATCA TTATTCTGTC CTGTGTAAGG 
ACAAGACAAG TAGGATTCAT TGATTCAGAA AAAAGAATGA ATGTTGCATT GACTAGAGGA 
AAGAGGCATT TGTTGATTGT GGGAAATTTA GCCTGTTTGA GGAAAAATCA ACTTTGGGGA 
CGAGTGATCC AACACTGCGA AGGAAGGGAA GATGGATTGC AACATGCAAA CCAGTATGAA 
CCACAGCTGA ACCATCTCCT TAAAGATTAT TTTGAAAAAC AAGTGGAAGA AAAACAGAAG 
AAAAAGAGTG AAAAAGAGAA ATCTAAAGAT AAATCTCATT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_011532097.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530399.1 Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X12, mRNA.

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CloneID	OHu69377
Clone ID Related Accession (Same CDS sequence)	XM_011532097.2
Accession Version	XM_011532097.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein ZGRF1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532097.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAGAG GACACAGCTC ACAGCTAGGA TGCAGTCAGT TTCCAGATAG CACTGAGTAT 
GAAAACTTTA TGACAGAGAC ACCAGAGTTA CCTAGCACGT GTATGCAGAT TGACTTCTTG 
CAGGTGACAT CACCAGAAGA AAACATCTCT ACATTGAGCC CTGTTTCTAC CTTTTCTTTG 
AACTCAAGAG ATGAAGACTT CATGGTAGAA TTCTCTGAGA CGTCCCTGAA AGCAAGAACT 
TTACCTGATG ATCTTCATTT TCTCAACTTG GAGGGGATGA AAAAATCCCG TTCTCTGGAG 
AATGAGAACC TTCAAAGGCT TTCATTATTA AGTAGAACCC AGGTTCCACT TATTACTTTG 
CCACGTACTG ATGGGCCACC TGACTTAGAC TCTCATTCGT ATATGATCAA CTCTAACACA 
TACGAGTCTT CTGGCTCCCC CATGCTCAAT TTGTGTGAAA AGTCAGCAGT TCTTTCGTTT 
AGCATTGAGC CTGAGGACCA AAATGAAACC TTTTTCTCTG AAGAATCTAG GGAAGTGAAT 
CCAGGGGATG TTTCACTTAA TAATATATCT ACTCAGAGCA AGTGGCTGAA ATATCAAAAC 
ACATCCCAAT GCAACGTGGC TACTCCAAAC AGAGTTGATA AGAGAATAAC TGATGGCTTC 
TTTGCTGAGG CTGTTTCTGG GATGCATTTT AGAGACACAA GTGAAAGACA GAGTGATGCT 
GTCAATGAAA GCTCTTTAGA CTCTGTGCAT TTGCAAATGA TAAAAGGCAT GCTCTATCAA 
CAGCGGCAGG ATTTTAGCAG TCAAGATTCG GTTTCCAGAA AGAAAGTACT TTCTCTGAAT 
TTAAAGCAGA CTTCTAAGAC AGAGGAAATT AAAAATGTAT TAGGAGGGTC TACCTGCTAC 
AACTACAGTG TAAAGGATTT ACAGGAGATA AGTGGCTCTG AGCTGTGCTT TCCAAGTGGG 
CAGAAAATAA AATCTGCTTA TCTTCCCCAA AGGCAAATTC ACATACCAGC TGTTTTTCAG 
TCTCCTGCTC ATTATAAGCA GACTTTCACA TCTTGCCTCA TAGAACATCT AAATATATTG 
CTGTTTGGGT TAGCACAAAA CCTGCAGAAA GCTCTTTCAA AAGTTGACAT ATCATTTTAT 
ACATCATTGA AGGGAGAGAA ACTGAAAAAC GCAGAAAATA ATGTACCATC CTGCCATCAT 
AGTCAACCTG CAAAACTTGT CATGGTTAAA AAGGAAGGTC CAAATAAGGG TCGTCTCTTT 
TATACATGTG ATGGACCCAA AGCTGATCGA TGTAAATTCT TTAAATGGCT TGAGGACGTG 
ACTCCAGGAT ATTCAACACA GGAAGGAGCT CGACCTGGCA TGGTTTTAAG TGATATTAAG 
AGTATTGGCT TATATTTAAG AAGTCAAAAG ATACCACTTT ATGAGGAATG CCAGCTTTTG 
GTGAGAAAAG GATTTGATTT TCAGAGAAAA CAGTATGGCA AACTAAAGAA GTTTACTACT 
GTAAATCCTG AGTTTTATAA TGAACCAAAA ACCAAACTTT ATCTTAAGCT AAGTCGGAAG 
GAAAGATCTT CAGCTTATAG CAAAAATGAT CTTTGGGTGG TTTCAAAAAC CCTAGACTTT 
GAGCTGGATA CTTTTATCGC ATGTAGTGCT TTCTTTGGAC CATCATCTAT CAATGAGATA 
GAAATACTGC CTTTGAAAGG CTATTTCCCT TCTAATTGGC CCACTAACAT GGTTGTCCAT 
GCGTTATTGG TTTGTAATGC TAGCACAGAA CTGACTACTT TGAAAAACAT TCAGGACTAC 
TTTAATCCAG CTACTCTACC TCTAACACAG TACCTGTTAA CAACGTCTTC GCCAACTATA 
GTTAGTAACA AAAGAGTCAG TAAGAGAAAA TTTATCCCAC CAGCCTTCAC AAATGTCAGT 
ACAAAATTTG AACTACTCAG CCTAGGAGCA ACATTGAAGT TAGCTAGTGA GTTGATTCAG 
GTACACAAGT TAAACAAGGA TCAAGCTACA GCTCTAATTC AAATAGCTCA AATGATGGCA 
TCACATGAAA GCATTGAAGA AGTGAAGGAA CTGCAAACTC ATACCTTCCC TATCACAATC 
ATACATGGTG TGTTTGGAGC AGGAAAGAGT TACTTGCTGG CAGTGGTGAT TTTGTTCTTT 
GTACAGCTGT TTGAAAAGAG TGAAGCTCCC ACCATTGGAA ATGCAAGGCC GTGGAAACTT 
CTGATTTCTT CTTCTACTAA TGTGGCTGTT GACAGAGTAC TTCTTGGGCT TCTCAGTCTT 
GGATTTGAAA ACTTTATCAG AGTTGGGAGT GTTAGGAAGA TTGCCAAACC AATTTTACCT 
TATAGCTTGC ATGCTGGCTC AGAAAATGAA AGTGAACAGT TAAAAGAACT ACATGCACTA 
ATGAAAGAAG ACCTGACTCC TACGGAAAGA GTCTATGTGA GAAAAAGCAT TGAGCAGCAT 
AAACTGGGGA CCAATAGAAC CCTGCTGAAG CAGGTTCGAG TAGTTGGAGT TACCTGTGCA 
GCCTGCCCAT TCCCATGCAT GAATGATCTT AAATTTCCTG TAGTTGTGCT GGATGAGTGT 
AGTCAGATAA CTGAACCGGC CTCTCTCCTT CCCATTGCAA GGTTTGAGTG TGAAAAGCTG 
ATTCTTGTTG GGGATCCCAA ACAGCTACCT CCTACTATTC AGGGTTCTGA TGCAGCTCAT 
GAAAATGGAT TGGAACAAAC TCTTTTTGAT CGACTTTGCT TAATGGGTCA CAAGCCAATT 
CTATTGAGAA CTCAATACCG TTGTCATCCT GCAATCAGTG CTATTGCTAA TGATCTGTTT 
TACAAAGGAG CCCTCATGAA TGGTGTAACA GAAATAGAGC GGAGCCCTTT ATTGGAATGG 
CTACCAACCC TGTGTTTTTA TAATGTTAAA GGACTAGAAC AGATAGAAAG AGATAACAGC 
TTTCATAATG TGGCAGAAGC TACGTTTACA CTCAAGCTGA TTCAATCACT GATTGCAAGT 
GGAATAGCAG GCTCTATGAT TGGTGTGATA ACATTATACA AATCCCAGAT GTACAAGCTT 
TGTCATTTAC TCAGTGCTGT GGACTTTCAC CATCCTGATA TTAAAACTGT GCAGGTGTCC 
ACAGTAGATG CTTTTCAGGG AGCTGAAAAG GAGATCATTA TTCTGTCCTG TGTAAGGACA 
AGACAAGTAG GATTCATTGA TTCAGAAAAA AGAATGAATG TTGCATTGAC TAGAGGAAAG 
AGGCATTTGT TGATTGTGGG AAATTTAGCC TGTTTGAGGA AAAATCAACT TTGGGGACGA 
GTGATCCAAC ACTGCGAAGG AAGGGAAGAT GGATTGCAAC ATGCAAACCA GTATGAACCA 
CAGCTGAACC ATCTCCTTAA AGATTATTTT GAAAAACAAG TGGAAGAAAA ACAGAAGAAA 
AAGAGTGAAA AAGAGAAATC TAAAGATAAA TCTCATTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530399.1
CDS	167..3628
Translation	MVRGHSSQLGCSQFPDSTEYENFMTETPELPSTCMQIDFLQVTSPEENISTLSPVSTFSLNSRDEDFMVEFSETSLKARTLPDDLHFLNLEGMKKSRSLENENLQRLSLLSRTQVPLITLPRTDGPPDLDSHSYMINSNTYESSGSPMLNLCEKSAVLSFSIEPEDQNETFFSEESREVNPGDVSLNNISTQSKWLKYQNTSQCNVATPNRVDKRITDGFFAEAVSGMHFRDTSERQSDAVNESSLDSVHLQMIKGMLYQQRQDFSSQDSVSRKKVLSLNLKQTSKTEEIKNVLGGSTCYNYSVKDLQEISGSELCFPSGQKIKSAYLPQRQIHIPAVFQSPAHYKQTFTSCLIEHLNILLFGLAQNLQKALSKVDISFYTSLKGEKLKNAENNVPSCHHSQPAKLVMVKKEGPNKGRLFYTCDGPKADRCKFFKWLEDVTPGYSTQEGARPGMVLSDIKSIGLYLRSQKIPLYEECQLLVRKGFDFQRKQYGKLKKFTTVNPEFYNEPKTKLYLKLSRKERSSAYSKNDLWVVSKTLDFELDTFIACSAFFGPSSINEIEILPLKGYFPSNWPTNMVVHALLVCNASTELTTLKNIQDYFNPATLPLTQYLLTTSSPTIVSNKRVSKRKFIPPAFTNVSTKFELLSLGATLKLASELIQVHKLNKDQATALIQIAQMMASHESIEEVKELQTHTFPITIIHGVFGAGKSYLLAVVILFFVQLFEKSEAPTIGNARPWKLLISSSTNVAVDRVLLGLLSLGFENFIRVGSVRKIAKPILPYSLHAGSENESEQLKELHALMKEDLTPTERVYVRKSIEQHKLGTNRTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPAISAIANDLFYKGALMNGVTEIERSPLLEWLPTLCFYNVKGLEQIERDNSFHNVAEATFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAVDFHHPDIKTVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGKRHLLIVGNLACLRKNQLWGRVIQHCEGREDGLQHANQYEPQLNHLLKDYFEKQVEEKQKKKSEKEKSKDKSHS

Target ORF information:

RefSeq Version	XM_011532097.2
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532097.2

ORF Insert Sequence:

ATGGTCAGAG GACACAGCTC ACAGCTAGGA TGCAGTCAGT TTCCAGATAG CACTGAGTAT 
GAAAACTTTA TGACAGAGAC ACCAGAGTTA CCTAGCACGT GTATGCAGAT TGACTTCTTG 
CAGGTGACAT CACCAGAAGA AAACATCTCT ACATTGAGCC CTGTTTCTAC CTTTTCTTTG 
AACTCAAGAG ATGAAGACTT CATGGTAGAA TTCTCTGAGA CGTCCCTGAA AGCAAGAACT 
TTACCTGATG ATCTTCATTT TCTCAACTTG GAGGGGATGA AAAAATCCCG TTCTCTGGAG 
AATGAGAACC TTCAAAGGCT TTCATTATTA AGTAGAACCC AGGTTCCACT TATTACTTTG 
CCACGTACTG ATGGGCCACC TGACTTAGAC TCTCATTCGT ATATGATCAA CTCTAACACA 
TACGAGTCTT CTGGCTCCCC CATGCTCAAT TTGTGTGAAA AGTCAGCAGT TCTTTCGTTT 
AGCATTGAGC CTGAGGACCA AAATGAAACC TTTTTCTCTG AAGAATCTAG GGAAGTGAAT 
CCAGGGGATG TTTCACTTAA TAATATATCT ACTCAGAGCA AGTGGCTGAA ATATCAAAAC 
ACATCCCAAT GCAACGTGGC TACTCCAAAC AGAGTTGATA AGAGAATAAC TGATGGCTTC 
TTTGCTGAGG CTGTTTCTGG GATGCATTTT AGAGACACAA GTGAAAGACA GAGTGATGCT 
GTCAATGAAA GCTCTTTAGA CTCTGTGCAT TTGCAAATGA TAAAAGGCAT GCTCTATCAA 
CAGCGGCAGG ATTTTAGCAG TCAAGATTCG GTTTCCAGAA AGAAAGTACT TTCTCTGAAT 
TTAAAGCAGA CTTCTAAGAC AGAGGAAATT AAAAATGTAT TAGGAGGGTC TACCTGCTAC 
AACTACAGTG TAAAGGATTT ACAGGAGATA AGTGGCTCTG AGCTGTGCTT TCCAAGTGGG 
CAGAAAATAA AATCTGCTTA TCTTCCCCAA AGGCAAATTC ACATACCAGC TGTTTTTCAG 
TCTCCTGCTC ATTATAAGCA GACTTTCACA TCTTGCCTCA TAGAACATCT AAATATATTG 
CTGTTTGGGT TAGCACAAAA CCTGCAGAAA GCTCTTTCAA AAGTTGACAT ATCATTTTAT 
ACATCATTGA AGGGAGAGAA ACTGAAAAAC GCAGAAAATA ATGTACCATC CTGCCATCAT 
AGTCAACCTG CAAAACTTGT CATGGTTAAA AAGGAAGGTC CAAATAAGGG TCGTCTCTTT 
TATACATGTG ATGGACCCAA AGCTGATCGA TGTAAATTCT TTAAATGGCT TGAGGACGTG 
ACTCCAGGAT ATTCAACACA GGAAGGAGCT CGACCTGGCA TGGTTTTAAG TGATATTAAG 
AGTATTGGCT TATATTTAAG AAGTCAAAAG ATACCACTTT ATGAGGAATG CCAGCTTTTG 
GTGAGAAAAG GATTTGATTT TCAGAGAAAA CAGTATGGCA AACTAAAGAA GTTTACTACT 
GTAAATCCTG AGTTTTATAA TGAACCAAAA ACCAAACTTT ATCTTAAGCT AAGTCGGAAG 
GAAAGATCTT CAGCTTATAG CAAAAATGAT CTTTGGGTGG TTTCAAAAAC CCTAGACTTT 
GAGCTGGATA CTTTTATCGC ATGTAGTGCT TTCTTTGGAC CATCATCTAT CAATGAGATA 
GAAATACTGC CTTTGAAAGG CTATTTCCCT TCTAATTGGC CCACTAACAT GGTTGTCCAT 
GCGTTATTGG TTTGTAATGC TAGCACAGAA CTGACTACTT TGAAAAACAT TCAGGACTAC 
TTTAATCCAG CTACTCTACC TCTAACACAG TACCTGTTAA CAACGTCTTC GCCAACTATA 
GTTAGTAACA AAAGAGTCAG TAAGAGAAAA TTTATCCCAC CAGCCTTCAC AAATGTCAGT 
ACAAAATTTG AACTACTCAG CCTAGGAGCA ACATTGAAGT TAGCTAGTGA GTTGATTCAG 
GTACACAAGT TAAACAAGGA TCAAGCTACA GCTCTAATTC AAATAGCTCA AATGATGGCA 
TCACATGAAA GCATTGAAGA AGTGAAGGAA CTGCAAACTC ATACCTTCCC TATCACAATC 
ATACATGGTG TGTTTGGAGC AGGAAAGAGT TACTTGCTGG CAGTGGTGAT TTTGTTCTTT 
GTACAGCTGT TTGAAAAGAG TGAAGCTCCC ACCATTGGAA ATGCAAGGCC GTGGAAACTT 
CTGATTTCTT CTTCTACTAA TGTGGCTGTT GACAGAGTAC TTCTTGGGCT TCTCAGTCTT 
GGATTTGAAA ACTTTATCAG AGTTGGGAGT GTTAGGAAGA TTGCCAAACC AATTTTACCT 
TATAGCTTGC ATGCTGGCTC AGAAAATGAA AGTGAACAGT TAAAAGAACT ACATGCACTA 
ATGAAAGAAG ACCTGACTCC TACGGAAAGA GTCTATGTGA GAAAAAGCAT TGAGCAGCAT 
AAACTGGGGA CCAATAGAAC CCTGCTGAAG CAGGTTCGAG TAGTTGGAGT TACCTGTGCA 
GCCTGCCCAT TCCCATGCAT GAATGATCTT AAATTTCCTG TAGTTGTGCT GGATGAGTGT 
AGTCAGATAA CTGAACCGGC CTCTCTCCTT CCCATTGCAA GGTTTGAGTG TGAAAAGCTG 
ATTCTTGTTG GGGATCCCAA ACAGCTACCT CCTACTATTC AGGGTTCTGA TGCAGCTCAT 
GAAAATGGAT TGGAACAAAC TCTTTTTGAT CGACTTTGCT TAATGGGTCA CAAGCCAATT 
CTATTGAGAA CTCAATACCG TTGTCATCCT GCAATCAGTG CTATTGCTAA TGATCTGTTT 
TACAAAGGAG CCCTCATGAA TGGTGTAACA GAAATAGAGC GGAGCCCTTT ATTGGAATGG 
CTACCAACCC TGTGTTTTTA TAATGTTAAA GGACTAGAAC AGATAGAAAG AGATAACAGC 
TTTCATAATG TGGCAGAAGC TACGTTTACA CTCAAGCTGA TTCAATCACT GATTGCAAGT 
GGAATAGCAG GCTCTATGAT TGGTGTGATA ACATTATACA AATCCCAGAT GTACAAGCTT 
TGTCATTTAC TCAGTGCTGT GGACTTTCAC CATCCTGATA TTAAAACTGT GCAGGTGTCC 
ACAGTAGATG CTTTTCAGGG AGCTGAAAAG GAGATCATTA TTCTGTCCTG TGTAAGGACA 
AGACAAGTAG GATTCATTGA TTCAGAAAAA AGAATGAATG TTGCATTGAC TAGAGGAAAG 
AGGCATTTGT TGATTGTGGG AAATTTAGCC TGTTTGAGGA AAAATCAACT TTGGGGACGA 
GTGATCCAAC ACTGCGAAGG AAGGGAAGAT GGATTGCAAC ATGCAAACCA GTATGAACCA 
CAGCTGAACC ATCTCCTTAA AGATTATTTT GAAAAACAAG TGGAAGAAAA ACAGAAGAAA 
AAGAGTGAAA AAGAGAAATC TAAAGATAAA TCTCATTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_011532099.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530401.1 Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu69378
Clone ID Related Accession (Same CDS sequence)	XM_011532099.2 , XM_011532098.2
Accession Version	XM_011532099.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein ZGRF1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532099.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAAT CCCGTTCTCT GGAGAATGAG AACCTTCAAA GGCTTTCATT ATTAAGTAGA 
ACCCAGGTTC CACTTATTAC TTTGCCACGT ACTGATGGGC CACCTGACTT AGACTCTCAT 
TCGTATATGA TCAACTCTAA CACATACGAG TCTTCTGGCT CCCCCATGCT CAATTTGTGT 
GAAAAGTCAG CAGTTCTTTC GTTTAGCATT GAGCCTGAGG ACCAAAATGA AACCTTTTTC 
TCTGAAGAAT CTAGGGAAGT GAATCCAGGG GATGTTTCAC TTAATAATAT ATCTACTCAG 
AGCAAGTGGC TGAAATATCA AAACACATCC CAATGCAACG TGGCTACTCC AAACAGAGTT 
GATAAGAGAA TAACTGATGG CTTCTTTGCT GAGGCTGTTT CTGGGATGCA TTTTAGAGAC 
ACAAGTGAAA GACAGAGTGA TGCTGTCAAT GAAAGCTCTT TAGACTCTGT GCATTTGCAA 
ATGATAAAAG GCATGCTCTA TCAACAGCGG CAGGATTTTA GCAGTCAAGA TTCGGTTTCC 
AGAAAGAAAG TACTTTCTCT GAATTTAAAG CAGACTTCTA AGACAGAGGA AATTAAAAAT 
GTATTAGGAG GGTCTACCTG CTACAACTAC AGTGTAAAGG ATTTACAGGA GATAAGTGGC 
TCTGAGCTGT GCTTTCCAAG TGGGCAGAAA ATAAAATCTG CTTATCTTCC CCAAAGGCAA 
ATTCACATAC CAGCTGTTTT TCAGTCTCCT GCTCATTATA AGCAGACTTT CACATCTTGC 
CTCATAGAAC ATCTAAATAT ATTGCTGTTT GGGTTAGCAC AAAACCTGCA GAAAGCTCTT 
TCAAAAGTTG ACATATCATT TTATACATCA TTGAAGGGAG AGAAACTGAA AAACGCAGAA 
AATAATGTAC CATCCTGCCA TCATAGTCAA CCTGCAAAAC TTGTCATGGT TAAAAAGGAA 
GGTCCAAATA AGGGTCGTCT CTTTTATACA TGTGATGGAC CCAAAGCTGA TCGATGTAAA 
TTCTTTAAAT GGCTTGAGGA CGTGACTCCA GGATATTCAA CACAGGAAGG AGCTCGACCT 
GGCATGGTTT TAAGTGATAT TAAGAGTATT GGCTTATATT TAAGAAGTCA AAAGATACCA 
CTTTATGAGG AATGCCAGCT TTTGGTGAGA AAAGGATTTG ATTTTCAGAG AAAACAGTAT 
GGCAAACTAA AGAAGTTTAC TACTGTAAAT CCTGAGTTTT ATAATGAACC AAAAACCAAA 
CTTTATCTTA AGCTAAGTCG GAAGGAAAGA TCTTCAGCTT ATAGCAAAAA TGATCTTTGG 
GTGGTTTCAA AAACCCTAGA CTTTGAGCTG GATACTTTTA TCGCATGTAG TGCTTTCTTT 
GGACCATCAT CTATCAATGA GATAGAAATA CTGCCTTTGA AAGGCTATTT CCCTTCTAAT 
TGGCCCACTA ACATGGTTGT CCATGCGTTA TTGGTTTGTA ATGCTAGCAC AGAACTGACT 
ACTTTGAAAA ACATTCAGGA CTACTTTAAT CCAGCTACTC TACCTCTAAC ACAGTACCTG 
TTAACAACGT CTTCGCCAAC TATAGTTAGT AACAAAAGAG TCAGTAAGAG AAAATTTATC 
CCACCAGCCT TCACAAATGT CAGTACAAAA TTTGAACTAC TCAGCCTAGG AGCAACATTG 
AAGTTAGCTA GTGAGTTGAT TCAGGTACAC AAGTTAAACA AGGATCAAGC TACAGCTCTA 
ATTCAAATAG CTCAAATGAT GGCATCACAT GAAAGCATTG AAGAAGTGAA GGAACTGCAA 
ACTCATACCT TCCCTATCAC AATCATACAT GGTGTGTTTG GAGCAGGAAA GAGTTACTTG 
CTGGCAGTGG TGATTTTGTT CTTTGTACAG CTGTTTGAAA AGAGTGAAGC TCCCACCATT 
GGAAATGCAA GGCCGTGGAA ACTTCTGATT TCTTCTTCTA CTAATGTGGC TGTTGACAGA 
GTACTTCTTG GGCTTCTCAG TCTTGGATTT GAAAACTTTA TCAGAGTTGG GAGTGTTAGG 
AAGATTGCCA AACCAATTTT ACCTTATAGC TTGCATGCTG GCTCAGAAAA TGAAAGTGAA 
CAGTTAAAAG AACTACATGC ACTAATGAAA GAAGACCTGA CTCCTACGGA AAGAGTCTAT 
GTGAGAAAAA GCATTGAGCA GCATAAACTG GGGACCAATA GAACCCTGCT GAAGCAGGTT 
CGAGTAGTTG GAGTTACCTG TGCAGCCTGC CCATTCCCAT GCATGAATGA TCTTAAATTT 
CCTGTAGTTG TGCTGGATGA GTGTAGTCAG ATAACTGAAC CGGCCTCTCT CCTTCCCATT 
GCAAGGTTTG AGTGTGAAAA GCTGATTCTT GTTGGGGATC CCAAACAGCT ACCTCCTACT 
ATTCAGGGTT CTGATGCAGC TCATGAAAAT GGATTGGAAC AAACTCTTTT TGATCGACTT 
TGCTTAATGG GTCACAAGCC AATTCTATTG AGAACTCAAT ACCGTTGTCA TCCTGCAATC 
AGTGCTATTG CTAATGATCT GTTTTACAAA GGAGCCCTCA TGAATGGTGT AACAGAAATA 
GAGCGGAGCC CTTTATTGGA ATGGCTACCA ACCCTGTGTT TTTATAATGT TAAAGGACTA 
GAACAGATAG AAAGAGATAA CAGCTTTCAT AATGTGGCAG AAGCTACGTT TACACTCAAG 
CTGATTCAAT CACTGATTGC AAGTGGAATA GCAGGCTCTA TGATTGGTGT GATAACATTA 
TACAAATCCC AGATGTACAA GCTTTGTCAT TTACTCAGTG CTGTGGACTT TCACCATCCT 
GATATTAAAA CTGTGCAGGT GTCCACAGTA GATGCTTTTC AGGGAGCTGA AAAGGAGATC 
ATTATTCTGT CCTGTGTAAG GACAAGACAA GTAGGATTCA TTGATTCAGA AAAAAGAATG 
AATGTTGCAT TGACTAGAGG AAAGAGGCAT TTGTTGATTG TGGGAAATTT AGCCTGTTTG 
AGGAAAAATC AACTTTGGGG ACGAGTGATC CAACACTGCG AAGGAAGGGA AGATGGATTG 
CAACATGCAA ACCAGTATGA ACCACAGCTG AACCATCTCC TTAAAGATTA TTTTGAAAAA 
CAAGTGGAAG AAAAACAGAA GAAAAAGAGT GAAAAAGAGA AATCTAAAGA TAAATCTCAT 
TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530401.1
CDS	76..3261
Translation	MKKSRSLENENLQRLSLLSRTQVPLITLPRTDGPPDLDSHSYMINSNTYESSGSPMLNLCEKSAVLSFSIEPEDQNETFFSEESREVNPGDVSLNNISTQSKWLKYQNTSQCNVATPNRVDKRITDGFFAEAVSGMHFRDTSERQSDAVNESSLDSVHLQMIKGMLYQQRQDFSSQDSVSRKKVLSLNLKQTSKTEEIKNVLGGSTCYNYSVKDLQEISGSELCFPSGQKIKSAYLPQRQIHIPAVFQSPAHYKQTFTSCLIEHLNILLFGLAQNLQKALSKVDISFYTSLKGEKLKNAENNVPSCHHSQPAKLVMVKKEGPNKGRLFYTCDGPKADRCKFFKWLEDVTPGYSTQEGARPGMVLSDIKSIGLYLRSQKIPLYEECQLLVRKGFDFQRKQYGKLKKFTTVNPEFYNEPKTKLYLKLSRKERSSAYSKNDLWVVSKTLDFELDTFIACSAFFGPSSINEIEILPLKGYFPSNWPTNMVVHALLVCNASTELTTLKNIQDYFNPATLPLTQYLLTTSSPTIVSNKRVSKRKFIPPAFTNVSTKFELLSLGATLKLASELIQVHKLNKDQATALIQIAQMMASHESIEEVKELQTHTFPITIIHGVFGAGKSYLLAVVILFFVQLFEKSEAPTIGNARPWKLLISSSTNVAVDRVLLGLLSLGFENFIRVGSVRKIAKPILPYSLHAGSENESEQLKELHALMKEDLTPTERVYVRKSIEQHKLGTNRTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPAISAIANDLFYKGALMNGVTEIERSPLLEWLPTLCFYNVKGLEQIERDNSFHNVAEATFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAVDFHHPDIKTVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGKRHLLIVGNLACLRKNQLWGRVIQHCEGREDGLQHANQYEPQLNHLLKDYFEKQVEEKQKKKSEKEKSKDKSHS

Target ORF information:

RefSeq Version	XM_011532099.2
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532099.2

ORF Insert Sequence:

ATGAAAAAAT CCCGTTCTCT GGAGAATGAG AACCTTCAAA GGCTTTCATT ATTAAGTAGA 
ACCCAGGTTC CACTTATTAC TTTGCCACGT ACTGATGGGC CACCTGACTT AGACTCTCAT 
TCGTATATGA TCAACTCTAA CACATACGAG TCTTCTGGCT CCCCCATGCT CAATTTGTGT 
GAAAAGTCAG CAGTTCTTTC GTTTAGCATT GAGCCTGAGG ACCAAAATGA AACCTTTTTC 
TCTGAAGAAT CTAGGGAAGT GAATCCAGGG GATGTTTCAC TTAATAATAT ATCTACTCAG 
AGCAAGTGGC TGAAATATCA AAACACATCC CAATGCAACG TGGCTACTCC AAACAGAGTT 
GATAAGAGAA TAACTGATGG CTTCTTTGCT GAGGCTGTTT CTGGGATGCA TTTTAGAGAC 
ACAAGTGAAA GACAGAGTGA TGCTGTCAAT GAAAGCTCTT TAGACTCTGT GCATTTGCAA 
ATGATAAAAG GCATGCTCTA TCAACAGCGG CAGGATTTTA GCAGTCAAGA TTCGGTTTCC 
AGAAAGAAAG TACTTTCTCT GAATTTAAAG CAGACTTCTA AGACAGAGGA AATTAAAAAT 
GTATTAGGAG GGTCTACCTG CTACAACTAC AGTGTAAAGG ATTTACAGGA GATAAGTGGC 
TCTGAGCTGT GCTTTCCAAG TGGGCAGAAA ATAAAATCTG CTTATCTTCC CCAAAGGCAA 
ATTCACATAC CAGCTGTTTT TCAGTCTCCT GCTCATTATA AGCAGACTTT CACATCTTGC 
CTCATAGAAC ATCTAAATAT ATTGCTGTTT GGGTTAGCAC AAAACCTGCA GAAAGCTCTT 
TCAAAAGTTG ACATATCATT TTATACATCA TTGAAGGGAG AGAAACTGAA AAACGCAGAA 
AATAATGTAC CATCCTGCCA TCATAGTCAA CCTGCAAAAC TTGTCATGGT TAAAAAGGAA 
GGTCCAAATA AGGGTCGTCT CTTTTATACA TGTGATGGAC CCAAAGCTGA TCGATGTAAA 
TTCTTTAAAT GGCTTGAGGA CGTGACTCCA GGATATTCAA CACAGGAAGG AGCTCGACCT 
GGCATGGTTT TAAGTGATAT TAAGAGTATT GGCTTATATT TAAGAAGTCA AAAGATACCA 
CTTTATGAGG AATGCCAGCT TTTGGTGAGA AAAGGATTTG ATTTTCAGAG AAAACAGTAT 
GGCAAACTAA AGAAGTTTAC TACTGTAAAT CCTGAGTTTT ATAATGAACC AAAAACCAAA 
CTTTATCTTA AGCTAAGTCG GAAGGAAAGA TCTTCAGCTT ATAGCAAAAA TGATCTTTGG 
GTGGTTTCAA AAACCCTAGA CTTTGAGCTG GATACTTTTA TCGCATGTAG TGCTTTCTTT 
GGACCATCAT CTATCAATGA GATAGAAATA CTGCCTTTGA AAGGCTATTT CCCTTCTAAT 
TGGCCCACTA ACATGGTTGT CCATGCGTTA TTGGTTTGTA ATGCTAGCAC AGAACTGACT 
ACTTTGAAAA ACATTCAGGA CTACTTTAAT CCAGCTACTC TACCTCTAAC ACAGTACCTG 
TTAACAACGT CTTCGCCAAC TATAGTTAGT AACAAAAGAG TCAGTAAGAG AAAATTTATC 
CCACCAGCCT TCACAAATGT CAGTACAAAA TTTGAACTAC TCAGCCTAGG AGCAACATTG 
AAGTTAGCTA GTGAGTTGAT TCAGGTACAC AAGTTAAACA AGGATCAAGC TACAGCTCTA 
ATTCAAATAG CTCAAATGAT GGCATCACAT GAAAGCATTG AAGAAGTGAA GGAACTGCAA 
ACTCATACCT TCCCTATCAC AATCATACAT GGTGTGTTTG GAGCAGGAAA GAGTTACTTG 
CTGGCAGTGG TGATTTTGTT CTTTGTACAG CTGTTTGAAA AGAGTGAAGC TCCCACCATT 
GGAAATGCAA GGCCGTGGAA ACTTCTGATT TCTTCTTCTA CTAATGTGGC TGTTGACAGA 
GTACTTCTTG GGCTTCTCAG TCTTGGATTT GAAAACTTTA TCAGAGTTGG GAGTGTTAGG 
AAGATTGCCA AACCAATTTT ACCTTATAGC TTGCATGCTG GCTCAGAAAA TGAAAGTGAA 
CAGTTAAAAG AACTACATGC ACTAATGAAA GAAGACCTGA CTCCTACGGA AAGAGTCTAT 
GTGAGAAAAA GCATTGAGCA GCATAAACTG GGGACCAATA GAACCCTGCT GAAGCAGGTT 
CGAGTAGTTG GAGTTACCTG TGCAGCCTGC CCATTCCCAT GCATGAATGA TCTTAAATTT 
CCTGTAGTTG TGCTGGATGA GTGTAGTCAG ATAACTGAAC CGGCCTCTCT CCTTCCCATT 
GCAAGGTTTG AGTGTGAAAA GCTGATTCTT GTTGGGGATC CCAAACAGCT ACCTCCTACT 
ATTCAGGGTT CTGATGCAGC TCATGAAAAT GGATTGGAAC AAACTCTTTT TGATCGACTT 
TGCTTAATGG GTCACAAGCC AATTCTATTG AGAACTCAAT ACCGTTGTCA TCCTGCAATC 
AGTGCTATTG CTAATGATCT GTTTTACAAA GGAGCCCTCA TGAATGGTGT AACAGAAATA 
GAGCGGAGCC CTTTATTGGA ATGGCTACCA ACCCTGTGTT TTTATAATGT TAAAGGACTA 
GAACAGATAG AAAGAGATAA CAGCTTTCAT AATGTGGCAG AAGCTACGTT TACACTCAAG 
CTGATTCAAT CACTGATTGC AAGTGGAATA GCAGGCTCTA TGATTGGTGT GATAACATTA 
TACAAATCCC AGATGTACAA GCTTTGTCAT TTACTCAGTG CTGTGGACTT TCACCATCCT 
GATATTAAAA CTGTGCAGGT GTCCACAGTA GATGCTTTTC AGGGAGCTGA AAAGGAGATC 
ATTATTCTGT CCTGTGTAAG GACAAGACAA GTAGGATTCA TTGATTCAGA AAAAAGAATG 
AATGTTGCAT TGACTAGAGG AAAGAGGCAT TTGTTGATTG TGGGAAATTT AGCCTGTTTG 
AGGAAAAATC AACTTTGGGG ACGAGTGATC CAACACTGCG AAGGAAGGGA AGATGGATTG 
CAACATGCAA ACCAGTATGA ACCACAGCTG AACCATCTCC TTAAAGATTA TTTTGAAAAA 
CAAGTGGAAG AAAAACAGAA GAAAAAGAGT GAAAAAGAGA AATCTAAAGA TAAATCTCAT 
TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_011532098.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530400.1 Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X13, mRNA.

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CloneID	OHu69378
Clone ID Related Accession (Same CDS sequence)	XM_011532099.2 , XM_011532098.2
Accession Version	XM_011532098.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein ZGRF1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532098.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAAT CCCGTTCTCT GGAGAATGAG AACCTTCAAA GGCTTTCATT ATTAAGTAGA 
ACCCAGGTTC CACTTATTAC TTTGCCACGT ACTGATGGGC CACCTGACTT AGACTCTCAT 
TCGTATATGA TCAACTCTAA CACATACGAG TCTTCTGGCT CCCCCATGCT CAATTTGTGT 
GAAAAGTCAG CAGTTCTTTC GTTTAGCATT GAGCCTGAGG ACCAAAATGA AACCTTTTTC 
TCTGAAGAAT CTAGGGAAGT GAATCCAGGG GATGTTTCAC TTAATAATAT ATCTACTCAG 
AGCAAGTGGC TGAAATATCA AAACACATCC CAATGCAACG TGGCTACTCC AAACAGAGTT 
GATAAGAGAA TAACTGATGG CTTCTTTGCT GAGGCTGTTT CTGGGATGCA TTTTAGAGAC 
ACAAGTGAAA GACAGAGTGA TGCTGTCAAT GAAAGCTCTT TAGACTCTGT GCATTTGCAA 
ATGATAAAAG GCATGCTCTA TCAACAGCGG CAGGATTTTA GCAGTCAAGA TTCGGTTTCC 
AGAAAGAAAG TACTTTCTCT GAATTTAAAG CAGACTTCTA AGACAGAGGA AATTAAAAAT 
GTATTAGGAG GGTCTACCTG CTACAACTAC AGTGTAAAGG ATTTACAGGA GATAAGTGGC 
TCTGAGCTGT GCTTTCCAAG TGGGCAGAAA ATAAAATCTG CTTATCTTCC CCAAAGGCAA 
ATTCACATAC CAGCTGTTTT TCAGTCTCCT GCTCATTATA AGCAGACTTT CACATCTTGC 
CTCATAGAAC ATCTAAATAT ATTGCTGTTT GGGTTAGCAC AAAACCTGCA GAAAGCTCTT 
TCAAAAGTTG ACATATCATT TTATACATCA TTGAAGGGAG AGAAACTGAA AAACGCAGAA 
AATAATGTAC CATCCTGCCA TCATAGTCAA CCTGCAAAAC TTGTCATGGT TAAAAAGGAA 
GGTCCAAATA AGGGTCGTCT CTTTTATACA TGTGATGGAC CCAAAGCTGA TCGATGTAAA 
TTCTTTAAAT GGCTTGAGGA CGTGACTCCA GGATATTCAA CACAGGAAGG AGCTCGACCT 
GGCATGGTTT TAAGTGATAT TAAGAGTATT GGCTTATATT TAAGAAGTCA AAAGATACCA 
CTTTATGAGG AATGCCAGCT TTTGGTGAGA AAAGGATTTG ATTTTCAGAG AAAACAGTAT 
GGCAAACTAA AGAAGTTTAC TACTGTAAAT CCTGAGTTTT ATAATGAACC AAAAACCAAA 
CTTTATCTTA AGCTAAGTCG GAAGGAAAGA TCTTCAGCTT ATAGCAAAAA TGATCTTTGG 
GTGGTTTCAA AAACCCTAGA CTTTGAGCTG GATACTTTTA TCGCATGTAG TGCTTTCTTT 
GGACCATCAT CTATCAATGA GATAGAAATA CTGCCTTTGA AAGGCTATTT CCCTTCTAAT 
TGGCCCACTA ACATGGTTGT CCATGCGTTA TTGGTTTGTA ATGCTAGCAC AGAACTGACT 
ACTTTGAAAA ACATTCAGGA CTACTTTAAT CCAGCTACTC TACCTCTAAC ACAGTACCTG 
TTAACAACGT CTTCGCCAAC TATAGTTAGT AACAAAAGAG TCAGTAAGAG AAAATTTATC 
CCACCAGCCT TCACAAATGT CAGTACAAAA TTTGAACTAC TCAGCCTAGG AGCAACATTG 
AAGTTAGCTA GTGAGTTGAT TCAGGTACAC AAGTTAAACA AGGATCAAGC TACAGCTCTA 
ATTCAAATAG CTCAAATGAT GGCATCACAT GAAAGCATTG AAGAAGTGAA GGAACTGCAA 
ACTCATACCT TCCCTATCAC AATCATACAT GGTGTGTTTG GAGCAGGAAA GAGTTACTTG 
CTGGCAGTGG TGATTTTGTT CTTTGTACAG CTGTTTGAAA AGAGTGAAGC TCCCACCATT 
GGAAATGCAA GGCCGTGGAA ACTTCTGATT TCTTCTTCTA CTAATGTGGC TGTTGACAGA 
GTACTTCTTG GGCTTCTCAG TCTTGGATTT GAAAACTTTA TCAGAGTTGG GAGTGTTAGG 
AAGATTGCCA AACCAATTTT ACCTTATAGC TTGCATGCTG GCTCAGAAAA TGAAAGTGAA 
CAGTTAAAAG AACTACATGC ACTAATGAAA GAAGACCTGA CTCCTACGGA AAGAGTCTAT 
GTGAGAAAAA GCATTGAGCA GCATAAACTG GGGACCAATA GAACCCTGCT GAAGCAGGTT 
CGAGTAGTTG GAGTTACCTG TGCAGCCTGC CCATTCCCAT GCATGAATGA TCTTAAATTT 
CCTGTAGTTG TGCTGGATGA GTGTAGTCAG ATAACTGAAC CGGCCTCTCT CCTTCCCATT 
GCAAGGTTTG AGTGTGAAAA GCTGATTCTT GTTGGGGATC CCAAACAGCT ACCTCCTACT 
ATTCAGGGTT CTGATGCAGC TCATGAAAAT GGATTGGAAC AAACTCTTTT TGATCGACTT 
TGCTTAATGG GTCACAAGCC AATTCTATTG AGAACTCAAT ACCGTTGTCA TCCTGCAATC 
AGTGCTATTG CTAATGATCT GTTTTACAAA GGAGCCCTCA TGAATGGTGT AACAGAAATA 
GAGCGGAGCC CTTTATTGGA ATGGCTACCA ACCCTGTGTT TTTATAATGT TAAAGGACTA 
GAACAGATAG AAAGAGATAA CAGCTTTCAT AATGTGGCAG AAGCTACGTT TACACTCAAG 
CTGATTCAAT CACTGATTGC AAGTGGAATA GCAGGCTCTA TGATTGGTGT GATAACATTA 
TACAAATCCC AGATGTACAA GCTTTGTCAT TTACTCAGTG CTGTGGACTT TCACCATCCT 
GATATTAAAA CTGTGCAGGT GTCCACAGTA GATGCTTTTC AGGGAGCTGA AAAGGAGATC 
ATTATTCTGT CCTGTGTAAG GACAAGACAA GTAGGATTCA TTGATTCAGA AAAAAGAATG 
AATGTTGCAT TGACTAGAGG AAAGAGGCAT TTGTTGATTG TGGGAAATTT AGCCTGTTTG 
AGGAAAAATC AACTTTGGGG ACGAGTGATC CAACACTGCG AAGGAAGGGA AGATGGATTG 
CAACATGCAA ACCAGTATGA ACCACAGCTG AACCATCTCC TTAAAGATTA TTTTGAAAAA 
CAAGTGGAAG AAAAACAGAA GAAAAAGAGT GAAAAAGAGA AATCTAAAGA TAAATCTCAT 
TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530400.1
CDS	117..3302
Translation	MKKSRSLENENLQRLSLLSRTQVPLITLPRTDGPPDLDSHSYMINSNTYESSGSPMLNLCEKSAVLSFSIEPEDQNETFFSEESREVNPGDVSLNNISTQSKWLKYQNTSQCNVATPNRVDKRITDGFFAEAVSGMHFRDTSERQSDAVNESSLDSVHLQMIKGMLYQQRQDFSSQDSVSRKKVLSLNLKQTSKTEEIKNVLGGSTCYNYSVKDLQEISGSELCFPSGQKIKSAYLPQRQIHIPAVFQSPAHYKQTFTSCLIEHLNILLFGLAQNLQKALSKVDISFYTSLKGEKLKNAENNVPSCHHSQPAKLVMVKKEGPNKGRLFYTCDGPKADRCKFFKWLEDVTPGYSTQEGARPGMVLSDIKSIGLYLRSQKIPLYEECQLLVRKGFDFQRKQYGKLKKFTTVNPEFYNEPKTKLYLKLSRKERSSAYSKNDLWVVSKTLDFELDTFIACSAFFGPSSINEIEILPLKGYFPSNWPTNMVVHALLVCNASTELTTLKNIQDYFNPATLPLTQYLLTTSSPTIVSNKRVSKRKFIPPAFTNVSTKFELLSLGATLKLASELIQVHKLNKDQATALIQIAQMMASHESIEEVKELQTHTFPITIIHGVFGAGKSYLLAVVILFFVQLFEKSEAPTIGNARPWKLLISSSTNVAVDRVLLGLLSLGFENFIRVGSVRKIAKPILPYSLHAGSENESEQLKELHALMKEDLTPTERVYVRKSIEQHKLGTNRTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPAISAIANDLFYKGALMNGVTEIERSPLLEWLPTLCFYNVKGLEQIERDNSFHNVAEATFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAVDFHHPDIKTVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGKRHLLIVGNLACLRKNQLWGRVIQHCEGREDGLQHANQYEPQLNHLLKDYFEKQVEEKQKKKSEKEKSKDKSHS

Target ORF information:

RefSeq Version	XM_011532098.2
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532098.2

ORF Insert Sequence:

ATGAAAAAAT CCCGTTCTCT GGAGAATGAG AACCTTCAAA GGCTTTCATT ATTAAGTAGA 
ACCCAGGTTC CACTTATTAC TTTGCCACGT ACTGATGGGC CACCTGACTT AGACTCTCAT 
TCGTATATGA TCAACTCTAA CACATACGAG TCTTCTGGCT CCCCCATGCT CAATTTGTGT 
GAAAAGTCAG CAGTTCTTTC GTTTAGCATT GAGCCTGAGG ACCAAAATGA AACCTTTTTC 
TCTGAAGAAT CTAGGGAAGT GAATCCAGGG GATGTTTCAC TTAATAATAT ATCTACTCAG 
AGCAAGTGGC TGAAATATCA AAACACATCC CAATGCAACG TGGCTACTCC AAACAGAGTT 
GATAAGAGAA TAACTGATGG CTTCTTTGCT GAGGCTGTTT CTGGGATGCA TTTTAGAGAC 
ACAAGTGAAA GACAGAGTGA TGCTGTCAAT GAAAGCTCTT TAGACTCTGT GCATTTGCAA 
ATGATAAAAG GCATGCTCTA TCAACAGCGG CAGGATTTTA GCAGTCAAGA TTCGGTTTCC 
AGAAAGAAAG TACTTTCTCT GAATTTAAAG CAGACTTCTA AGACAGAGGA AATTAAAAAT 
GTATTAGGAG GGTCTACCTG CTACAACTAC AGTGTAAAGG ATTTACAGGA GATAAGTGGC 
TCTGAGCTGT GCTTTCCAAG TGGGCAGAAA ATAAAATCTG CTTATCTTCC CCAAAGGCAA 
ATTCACATAC CAGCTGTTTT TCAGTCTCCT GCTCATTATA AGCAGACTTT CACATCTTGC 
CTCATAGAAC ATCTAAATAT ATTGCTGTTT GGGTTAGCAC AAAACCTGCA GAAAGCTCTT 
TCAAAAGTTG ACATATCATT TTATACATCA TTGAAGGGAG AGAAACTGAA AAACGCAGAA 
AATAATGTAC CATCCTGCCA TCATAGTCAA CCTGCAAAAC TTGTCATGGT TAAAAAGGAA 
GGTCCAAATA AGGGTCGTCT CTTTTATACA TGTGATGGAC CCAAAGCTGA TCGATGTAAA 
TTCTTTAAAT GGCTTGAGGA CGTGACTCCA GGATATTCAA CACAGGAAGG AGCTCGACCT 
GGCATGGTTT TAAGTGATAT TAAGAGTATT GGCTTATATT TAAGAAGTCA AAAGATACCA 
CTTTATGAGG AATGCCAGCT TTTGGTGAGA AAAGGATTTG ATTTTCAGAG AAAACAGTAT 
GGCAAACTAA AGAAGTTTAC TACTGTAAAT CCTGAGTTTT ATAATGAACC AAAAACCAAA 
CTTTATCTTA AGCTAAGTCG GAAGGAAAGA TCTTCAGCTT ATAGCAAAAA TGATCTTTGG 
GTGGTTTCAA AAACCCTAGA CTTTGAGCTG GATACTTTTA TCGCATGTAG TGCTTTCTTT 
GGACCATCAT CTATCAATGA GATAGAAATA CTGCCTTTGA AAGGCTATTT CCCTTCTAAT 
TGGCCCACTA ACATGGTTGT CCATGCGTTA TTGGTTTGTA ATGCTAGCAC AGAACTGACT 
ACTTTGAAAA ACATTCAGGA CTACTTTAAT CCAGCTACTC TACCTCTAAC ACAGTACCTG 
TTAACAACGT CTTCGCCAAC TATAGTTAGT AACAAAAGAG TCAGTAAGAG AAAATTTATC 
CCACCAGCCT TCACAAATGT CAGTACAAAA TTTGAACTAC TCAGCCTAGG AGCAACATTG 
AAGTTAGCTA GTGAGTTGAT TCAGGTACAC AAGTTAAACA AGGATCAAGC TACAGCTCTA 
ATTCAAATAG CTCAAATGAT GGCATCACAT GAAAGCATTG AAGAAGTGAA GGAACTGCAA 
ACTCATACCT TCCCTATCAC AATCATACAT GGTGTGTTTG GAGCAGGAAA GAGTTACTTG 
CTGGCAGTGG TGATTTTGTT CTTTGTACAG CTGTTTGAAA AGAGTGAAGC TCCCACCATT 
GGAAATGCAA GGCCGTGGAA ACTTCTGATT TCTTCTTCTA CTAATGTGGC TGTTGACAGA 
GTACTTCTTG GGCTTCTCAG TCTTGGATTT GAAAACTTTA TCAGAGTTGG GAGTGTTAGG 
AAGATTGCCA AACCAATTTT ACCTTATAGC TTGCATGCTG GCTCAGAAAA TGAAAGTGAA 
CAGTTAAAAG AACTACATGC ACTAATGAAA GAAGACCTGA CTCCTACGGA AAGAGTCTAT 
GTGAGAAAAA GCATTGAGCA GCATAAACTG GGGACCAATA GAACCCTGCT GAAGCAGGTT 
CGAGTAGTTG GAGTTACCTG TGCAGCCTGC CCATTCCCAT GCATGAATGA TCTTAAATTT 
CCTGTAGTTG TGCTGGATGA GTGTAGTCAG ATAACTGAAC CGGCCTCTCT CCTTCCCATT 
GCAAGGTTTG AGTGTGAAAA GCTGATTCTT GTTGGGGATC CCAAACAGCT ACCTCCTACT 
ATTCAGGGTT CTGATGCAGC TCATGAAAAT GGATTGGAAC AAACTCTTTT TGATCGACTT 
TGCTTAATGG GTCACAAGCC AATTCTATTG AGAACTCAAT ACCGTTGTCA TCCTGCAATC 
AGTGCTATTG CTAATGATCT GTTTTACAAA GGAGCCCTCA TGAATGGTGT AACAGAAATA 
GAGCGGAGCC CTTTATTGGA ATGGCTACCA ACCCTGTGTT TTTATAATGT TAAAGGACTA 
GAACAGATAG AAAGAGATAA CAGCTTTCAT AATGTGGCAG AAGCTACGTT TACACTCAAG 
CTGATTCAAT CACTGATTGC AAGTGGAATA GCAGGCTCTA TGATTGGTGT GATAACATTA 
TACAAATCCC AGATGTACAA GCTTTGTCAT TTACTCAGTG CTGTGGACTT TCACCATCCT 
GATATTAAAA CTGTGCAGGT GTCCACAGTA GATGCTTTTC AGGGAGCTGA AAAGGAGATC 
ATTATTCTGT CCTGTGTAAG GACAAGACAA GTAGGATTCA TTGATTCAGA AAAAAGAATG 
AATGTTGCAT TGACTAGAGG AAAGAGGCAT TTGTTGATTG TGGGAAATTT AGCCTGTTTG 
AGGAAAAATC AACTTTGGGG ACGAGTGATC CAACACTGCG AAGGAAGGGA AGATGGATTG 
CAACATGCAA ACCAGTATGA ACCACAGCTG AACCATCTCC TTAAAGATTA TTTTGAAAAA 
CAAGTGGAAG AAAAACAGAA GAAAAAGAGT GAAAAAGAGA AATCTAAAGA TAAATCTCAT 
TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_011532096.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530398.1 Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu69376
Clone ID Related Accession (Same CDS sequence)	XM_011532096.2
Accession Version	XM_011532096.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein ZGRF1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532096.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGCC AAGAATTTAT TGTTCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAATTTT ATATGATGAC 
AAAGGAGCAT GTTTGGAGAG TCTGTTTCTT AAATGCCTTG AGGTGAAACC TGGAGATGAC 
TTAGAAAGTG ATCGATACTT AATCACAGTT GAAGAGGTTA AAGTTGCTGG AGCCATAGGT 
ATTGTTAAGC AGAATGTCAA TAAAGAAGCA CCAGAGTTAA ATTCAAGGAC ATTTATATCC 
TCTGGCCGAT CTCTTGGATG TCAGCCCTCT GGCTTAAAAA GGAAGTTTAC TGGTTTTCAA 
GGACCACGTC AGGTTCCAAA GAAAATGGTT ATTATGGAAA GTGGTGAATC AGCTGCATCA 
CATGAGGCTA AGAAAACTGG CCCTACTATT TTTTCTCCAT TCTGCAGCAT GCCTCCTTTG 
TTTCCTACTG TTGGCAAGAA AGATGTAAAT AATATACTGG CAGACCCTGA GAACATTGTG 
ACTTACAAGA ACAGGGAGAG AAATGCCATG GATTTTTCTT CGGTTTTTTC TCCATCCTTC 
CAGATTAACC CAGAAGTGCT GTGTGAAGAA AATTATTTTT GCTCACCTGT CAATTCTGGA 
AATAAGCTTT CAGACTCTTT ACTGACCAAT GAGCCTGTGA AAAGAGATAG TTTGGCATCT 
CACTATTCAG GAGTTTCACA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTGAAG 
TCCGAATCAT CTAGTTCATG TGAGGAACTA AATTCTGAGA TGACAGAGCA TTTTCCTCAA 
AAACAACCAC AAGGAAGTTT AAAAATTGCT ACTAAACCAA AGTACCTAAT TCAACAGGAA 
GAGTGTGCTG AGATGAAGAG CACAGAAAAT TTATACTACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TATACATTCT 
TCTACTGTAG ATGGGAATGA TACAGAAAGG AAACCCAAGG CCCAGGAAGA TGATGTAAAT 
TCTAATTTGA AAGACCTTTC ATTACAAAAA ATTATACAGT TCGTTGAAAC GTATGCTGAA 
GAGAGGAAAA AGTATAATGT AGACCAGTCA GTCGGTAATA ATGATCCATC CTGGAATCAG 
GAAGTAAAAT TAGAAATTCC TTCATTCAAT GAAAGCAGTA GCTTACAGGT TACTTGTAGC 
AGTGCAGAAA ATGATGGTAT ATTATCAGAA TCTGACATTC AAGAAGATAA TAAAATACCT 
TTTAATCAAA ATGACAAGGG GTGCATTAAA GGATCAGTTC TCATTAAAGA AAATGCTCAG 
GAGGTAAATA CATGTGGAAC ACTGGAAAAG GAGTATGAAC AATCAGAGTC ATCTCTGCCA 
GAACTGAAAC ATCTCCAAAT TGAATCTAGT AATAATTCTA GGATCTCTGA TGACATTACA 
GACATGATTT CTGAAAGTAA AATGGATAAT GAAAGTCTTA ATAGTATTCA TGAATCTCTG 
AGTAATGTAA CACAACCATT TTTGGAGGTA ACTTTTAATC TGAACAATTT TGAGACCAGT 
GACACTGAGG AGGAATCACA GGAAAGCAAC AAAATTTCCC AGGATTCAGA GAGTTGGGTA 
AAGGACATTT TGGTTAATGA TGGCAATTCA TGTTTTCAAA AGAGGTCTGA GAATACAAAC 
TGTGAAGAGA TTGAGGGTGA ACATTTGCCA TTCTTAACAT CTGTTAGTGA CAAACCTACA 
GTGACATTTC CTGTTAAAGA GACTCTGCCA TCACAGTTTT GTGATAAAAC TTATGTGGGT 
TTTGACATGG GAATATGTAA AACTGAAAAC ACAGGAAAAG AAATAGAGGA GTATAGTGAC 
ACATTAAGCA ATTTTGAATC TTTCAAGTGG ACTGATGCTG TATACGGAGA TAATAAAGAA 
GATGCTAATA AACCTATTCA AGAAGTCAGA ATTAACTATG ATTTTGCTTT ACCCCCGAAT 
AAATCTAAAG GTATAAACAT GAATTTACAT ATTCCTCATA TTCAAAACCA GATTGCTGAA 
AACAGTAATC TATTTTCAGA AGATGCTCAA CCTCAGCCTT TTATTTTGGG AAGTGACTTA 
GACAAAAATG ATGAACATGT TTTACCCTCA ACTTCTAGTA GTGACAACAG TGTCCAACTA 
TTAAATACCA ATCAGAATCA CTATGAATGT ATTGCACTTG ATAAATCAAA TACCCACATT 
TCCAATTCTT TGTTTTACCC ACTGGGAAAA AAGCATCTTA TTTCCAAAGA CACAGAAGCA 
CATATATCTG AACCTGAAGA TTTGGGAAAG ATTAGAAGTC CACCCCCTGA CCATGTTGAG 
GTTGAAACTG CCAGAGAAGG CAAACAATAC TGGAATCCTA GAAATTCTTC AGAACTTTCT 
GGATTAGTAA ATACCATTTC TATTTTAAAG TCGCTATGTG AACACAGTAC TGCTTTAGAC 
AGCTTGGAAA TATTGAAAAA GAAAAATACT GTCTTTCAGC AAGGAACTCA ACAGACGTAT 
GAGCCAGATA GCCCTCCTGA AGTGAGGAAA CCATTTATTA CAGTAGTTTC ACCAAAGTCT 
CCTCATCTGC ACAAGGATTC TCAACAGATA CTAAAAGAAG ATGAAGTTGA ACTAAGTGAA 
CCACTTCAGT CTGTGCAGTT CTCTTCCTCG GGAAGTAAAG AAGAGACTGC TTTTCAAGCT 
GTTATTCCTA AACAAATAGA GAGAAAAACC TGTGACCCTA AGCCTGTTGA GTTTCAAGGA 
CATCAAGTAA AAGGATCAGC TACTAGTGGT GTAATGGTCA GAGGACACAG CTCACAGCTA 
GGATGCAGTC AGTTTCCAGA TAGCACTGAG TATGAAAACT TTATGACAGA GACACCAGAG 
TTACCTAGCA CGTGTATGCA GATTGACTTC TTGCAGGTGA CATCACCAGA AGAAAACATC 
TCTACATTGA GCCCTGTTTC TACCTTTTCT TTGAACTCAA GAGATGAAGA CTTCATGGTA 
GAATTCTCTG AGACGTCCCT GAAAGCAAGA ACTTTACCTG ATGATCTTCA TTTTCTCAAC 
TTGGAGGGGA TGAAAAAATC CCGTTCTCTG GAGAATGAGA ACCTTCAAAG GCTTTCATTA 
TTAAGTAGAA CCCAGGTTCC ACTTATTACT TTGCCACGTA CTGATGGGCC ACCTGACTTA 
GACTCTCATT CGTATATGAT CAACTCTAAC ACATACGAGT CTTCTGGCTC CCCCATGCTC 
AATTTGTGTG AAAAGTCAGC AGTTCTTTCG TTTAGCATTG AGCCTGAGGA CCAAAATGAA 
ACCTTTTTCT CTGAAGAATC TAGGGAAGTG AATCCAGGGG ATGTTTCACT TAATAATATA 
TCTACTCAGA GCAAGTGGCT GAAATATCAA AACACATCCC AATGCAACGT GGCTACTCCA 
AACAGAGTTG ATAAGAGAAT AACTGATGGC TTCTTTGCTG AGGCTGTTTC TGGGATGCAT 
TTTAGAGACA CAAGTGAAAG ACAGAGTGAT GCTGTCAATG AAAGCTCTTT AGACTCTGTG 
CATTTGCAAA TGATAAAAGG CATGCTCTAT CAACAGCGGC AGGATTTTAG CAGTCAAGAT 
TCGGTTTCCA GAAAGAAAGT ACTTTCTCTG AATTTAAAGC AGACTTCTAA GACAGAGGAA 
ATTAAAAATG TATTAGGAGG GTCTACCTGC TACAACTACA GTGTAAAGGA TTTACAGGAG 
ATAAGTGGCT CTGAGCTGTG CTTTCCAAGT GGGCAGAAAA TAAAATCTGC TTATCTTCCC 
CAAAGGCAAA TTCACATACC AGCTGTTTTT CAGTCTCCTG CTCATTATAA GCAGACTTTC 
ACATCTTGCC TCATAGAACA TCTAAATATA TTGCTGTTTG GGTTAGCACA AAACCTGCAG 
AAAGCTCTTT CAAAAGTTGA CATATCATTT TATACATCAT TGAAGGGAGA GAAACTGAAA 
AACGCAGAAA ATAATGTACC ATCCTGCCAT CATAGTCAAC CTGCAAAACT TGTCATGGTT 
AAAAAGGAAG GTCCAAATAA GGGTCGTCTC TTTTATACAT GTGATGGACC CAAAGCTGAT 
CGATGTAAAT TCTTTAAATG GCTTGAGGAC GTGACTCCAG GATATTCAAC ACAGGAAGGA 
GCTCGACCTG GCATGGTTTT AAGTGATATT AAGAGTATTG GCTTATATTT AAGAAGTCAA 
AAGATACCAC TTTATGAGGA ATGCCAGCTT TTGGTGAGAA AAGGATTTGA TTTTCAGAGA 
AAACAGTATG GCAAACTAAA GAAGTTTACT ACTGTAAATC CTGAGTTTTA TAATGAACCA 
AAAACCAAAC TTTATCTTAA GCTAAGTCGG AAGGAAAGAT CTTCAGCTTA TAGCAAAAAT 
GATCTTTGGG TGGTTTCAAA AACCCTAGAC TTTGAGCTGG ATACTTTTAT CGCATGTAGT 
GCTTTCTTTG GACCATCATC TATCAATGAG ATAGAAATAC TGCCTTTGAA AGGCTATTTC 
CCTTCTAATT GGCCCACTAA CATGGTTGTC CATGCGTTAT TGGTTTGTAA TGCTAGCACA 
GAACTGACTA CTTTGAAAAA CATTCAGGAC TACTTTAATC CAGCTACTCT ACCTCTAACA 
CAGTACCTGT TAACAACTGT TGTTGTGAAG ATTTCATCAA GTAATATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530398.1
CDS	212..4942
Translation	MESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGACLESLFLKCLEVKPGDDLESDRYLITVEEVKVAGAIGIVKQNVNKEAPELNSRTFISSGRSLGCQPSGLKRKFTGFQGPRQVPKKMVIMESGESAASHEAKKTGPTIFSPFCSMPPLFPTVGKKDVNNILADPENIVTYKNRERNAMDFSSVFSPSFQINPEVLCEENYFCSPVNSGNKLSDSLLTNEPVKRDSLASHYSGVSQNIRSKAQILALLKSESSSSCEELNSEMTEHFPQKQPQGSLKIATKPKYLIQQEECAEMKSTENLYYQHQSENTMRNKSRWAMYLSSQSSPIHSSTVDGNDTERKPKAQEDDVNSNLKDLSLQKIIQFVETYAEERKKYNVDQSVGNNDPSWNQEVKLEIPSFNESSSLQVTCSSAENDGILSESDIQEDNKIPFNQNDKGCIKGSVLIKENAQEVNTCGTLEKEYEQSESSLPELKHLQIESSNNSRISDDITDMISESKMDNESLNSIHESLSNVTQPFLEVTFNLNNFETSDTEEESQESNKISQDSESWVKDILVNDGNSCFQKRSENTNCEEIEGEHLPFLTSVSDKPTVTFPVKETLPSQFCDKTYVGFDMGICKTENTGKEIEEYSDTLSNFESFKWTDAVYGDNKEDANKPIQEVRINYDFALPPNKSKGINMNLHIPHIQNQIAENSNLFSEDAQPQPFILGSDLDKNDEHVLPSTSSSDNSVQLLNTNQNHYECIALDKSNTHISNSLFYPLGKKHLISKDTEAHISEPEDLGKIRSPPPDHVEVETAREGKQYWNPRNSSELSGLVNTISILKSLCEHSTALDSLEILKKKNTVFQQGTQQTYEPDSPPEVRKPFITVVSPKSPHLHKDSQQILKEDEVELSEPLQSVQFSSSGSKEETAFQAVIPKQIERKTCDPKPVEFQGHQVKGSATSGVMVRGHSSQLGCSQFPDSTEYENFMTETPELPSTCMQIDFLQVTSPEENISTLSPVSTFSLNSRDEDFMVEFSETSLKARTLPDDLHFLNLEGMKKSRSLENENLQRLSLLSRTQVPLITLPRTDGPPDLDSHSYMINSNTYESSGSPMLNLCEKSAVLSFSIEPEDQNETFFSEESREVNPGDVSLNNISTQSKWLKYQNTSQCNVATPNRVDKRITDGFFAEAVSGMHFRDTSERQSDAVNESSLDSVHLQMIKGMLYQQRQDFSSQDSVSRKKVLSLNLKQTSKTEEIKNVLGGSTCYNYSVKDLQEISGSELCFPSGQKIKSAYLPQRQIHIPAVFQSPAHYKQTFTSCLIEHLNILLFGLAQNLQKALSKVDISFYTSLKGEKLKNAENNVPSCHHSQPAKLVMVKKEGPNKGRLFYTCDGPKADRCKFFKWLEDVTPGYSTQEGARPGMVLSDIKSIGLYLRSQKIPLYEECQLLVRKGFDFQRKQYGKLKKFTTVNPEFYNEPKTKLYLKLSRKERSSAYSKNDLWVVSKTLDFELDTFIACSAFFGPSSINEIEILPLKGYFPSNWPTNMVVHALLVCNASTELTTLKNIQDYFNPATLPLTQYLLTTVVVKISSSNM

Target ORF information:

RefSeq Version	XM_011532096.2
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532096.2

ORF Insert Sequence:

ATGGAAAGCC AAGAATTTAT TGTTCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAATTTT ATATGATGAC 
AAAGGAGCAT GTTTGGAGAG TCTGTTTCTT AAATGCCTTG AGGTGAAACC TGGAGATGAC 
TTAGAAAGTG ATCGATACTT AATCACAGTT GAAGAGGTTA AAGTTGCTGG AGCCATAGGT 
ATTGTTAAGC AGAATGTCAA TAAAGAAGCA CCAGAGTTAA ATTCAAGGAC ATTTATATCC 
TCTGGCCGAT CTCTTGGATG TCAGCCCTCT GGCTTAAAAA GGAAGTTTAC TGGTTTTCAA 
GGACCACGTC AGGTTCCAAA GAAAATGGTT ATTATGGAAA GTGGTGAATC AGCTGCATCA 
CATGAGGCTA AGAAAACTGG CCCTACTATT TTTTCTCCAT TCTGCAGCAT GCCTCCTTTG 
TTTCCTACTG TTGGCAAGAA AGATGTAAAT AATATACTGG CAGACCCTGA GAACATTGTG 
ACTTACAAGA ACAGGGAGAG AAATGCCATG GATTTTTCTT CGGTTTTTTC TCCATCCTTC 
CAGATTAACC CAGAAGTGCT GTGTGAAGAA AATTATTTTT GCTCACCTGT CAATTCTGGA 
AATAAGCTTT CAGACTCTTT ACTGACCAAT GAGCCTGTGA AAAGAGATAG TTTGGCATCT 
CACTATTCAG GAGTTTCACA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTGAAG 
TCCGAATCAT CTAGTTCATG TGAGGAACTA AATTCTGAGA TGACAGAGCA TTTTCCTCAA 
AAACAACCAC AAGGAAGTTT AAAAATTGCT ACTAAACCAA AGTACCTAAT TCAACAGGAA 
GAGTGTGCTG AGATGAAGAG CACAGAAAAT TTATACTACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TATACATTCT 
TCTACTGTAG ATGGGAATGA TACAGAAAGG AAACCCAAGG CCCAGGAAGA TGATGTAAAT 
TCTAATTTGA AAGACCTTTC ATTACAAAAA ATTATACAGT TCGTTGAAAC GTATGCTGAA 
GAGAGGAAAA AGTATAATGT AGACCAGTCA GTCGGTAATA ATGATCCATC CTGGAATCAG 
GAAGTAAAAT TAGAAATTCC TTCATTCAAT GAAAGCAGTA GCTTACAGGT TACTTGTAGC 
AGTGCAGAAA ATGATGGTAT ATTATCAGAA TCTGACATTC AAGAAGATAA TAAAATACCT 
TTTAATCAAA ATGACAAGGG GTGCATTAAA GGATCAGTTC TCATTAAAGA AAATGCTCAG 
GAGGTAAATA CATGTGGAAC ACTGGAAAAG GAGTATGAAC AATCAGAGTC ATCTCTGCCA 
GAACTGAAAC ATCTCCAAAT TGAATCTAGT AATAATTCTA GGATCTCTGA TGACATTACA 
GACATGATTT CTGAAAGTAA AATGGATAAT GAAAGTCTTA ATAGTATTCA TGAATCTCTG 
AGTAATGTAA CACAACCATT TTTGGAGGTA ACTTTTAATC TGAACAATTT TGAGACCAGT 
GACACTGAGG AGGAATCACA GGAAAGCAAC AAAATTTCCC AGGATTCAGA GAGTTGGGTA 
AAGGACATTT TGGTTAATGA TGGCAATTCA TGTTTTCAAA AGAGGTCTGA GAATACAAAC 
TGTGAAGAGA TTGAGGGTGA ACATTTGCCA TTCTTAACAT CTGTTAGTGA CAAACCTACA 
GTGACATTTC CTGTTAAAGA GACTCTGCCA TCACAGTTTT GTGATAAAAC TTATGTGGGT 
TTTGACATGG GAATATGTAA AACTGAAAAC ACAGGAAAAG AAATAGAGGA GTATAGTGAC 
ACATTAAGCA ATTTTGAATC TTTCAAGTGG ACTGATGCTG TATACGGAGA TAATAAAGAA 
GATGCTAATA AACCTATTCA AGAAGTCAGA ATTAACTATG ATTTTGCTTT ACCCCCGAAT 
AAATCTAAAG GTATAAACAT GAATTTACAT ATTCCTCATA TTCAAAACCA GATTGCTGAA 
AACAGTAATC TATTTTCAGA AGATGCTCAA CCTCAGCCTT TTATTTTGGG AAGTGACTTA 
GACAAAAATG ATGAACATGT TTTACCCTCA ACTTCTAGTA GTGACAACAG TGTCCAACTA 
TTAAATACCA ATCAGAATCA CTATGAATGT ATTGCACTTG ATAAATCAAA TACCCACATT 
TCCAATTCTT TGTTTTACCC ACTGGGAAAA AAGCATCTTA TTTCCAAAGA CACAGAAGCA 
CATATATCTG AACCTGAAGA TTTGGGAAAG ATTAGAAGTC CACCCCCTGA CCATGTTGAG 
GTTGAAACTG CCAGAGAAGG CAAACAATAC TGGAATCCTA GAAATTCTTC AGAACTTTCT 
GGATTAGTAA ATACCATTTC TATTTTAAAG TCGCTATGTG AACACAGTAC TGCTTTAGAC 
AGCTTGGAAA TATTGAAAAA GAAAAATACT GTCTTTCAGC AAGGAACTCA ACAGACGTAT 
GAGCCAGATA GCCCTCCTGA AGTGAGGAAA CCATTTATTA CAGTAGTTTC ACCAAAGTCT 
CCTCATCTGC ACAAGGATTC TCAACAGATA CTAAAAGAAG ATGAAGTTGA ACTAAGTGAA 
CCACTTCAGT CTGTGCAGTT CTCTTCCTCG GGAAGTAAAG AAGAGACTGC TTTTCAAGCT 
GTTATTCCTA AACAAATAGA GAGAAAAACC TGTGACCCTA AGCCTGTTGA GTTTCAAGGA 
CATCAAGTAA AAGGATCAGC TACTAGTGGT GTAATGGTCA GAGGACACAG CTCACAGCTA 
GGATGCAGTC AGTTTCCAGA TAGCACTGAG TATGAAAACT TTATGACAGA GACACCAGAG 
TTACCTAGCA CGTGTATGCA GATTGACTTC TTGCAGGTGA CATCACCAGA AGAAAACATC 
TCTACATTGA GCCCTGTTTC TACCTTTTCT TTGAACTCAA GAGATGAAGA CTTCATGGTA 
GAATTCTCTG AGACGTCCCT GAAAGCAAGA ACTTTACCTG ATGATCTTCA TTTTCTCAAC 
TTGGAGGGGA TGAAAAAATC CCGTTCTCTG GAGAATGAGA ACCTTCAAAG GCTTTCATTA 
TTAAGTAGAA CCCAGGTTCC ACTTATTACT TTGCCACGTA CTGATGGGCC ACCTGACTTA 
GACTCTCATT CGTATATGAT CAACTCTAAC ACATACGAGT CTTCTGGCTC CCCCATGCTC 
AATTTGTGTG AAAAGTCAGC AGTTCTTTCG TTTAGCATTG AGCCTGAGGA CCAAAATGAA 
ACCTTTTTCT CTGAAGAATC TAGGGAAGTG AATCCAGGGG ATGTTTCACT TAATAATATA 
TCTACTCAGA GCAAGTGGCT GAAATATCAA AACACATCCC AATGCAACGT GGCTACTCCA 
AACAGAGTTG ATAAGAGAAT AACTGATGGC TTCTTTGCTG AGGCTGTTTC TGGGATGCAT 
TTTAGAGACA CAAGTGAAAG ACAGAGTGAT GCTGTCAATG AAAGCTCTTT AGACTCTGTG 
CATTTGCAAA TGATAAAAGG CATGCTCTAT CAACAGCGGC AGGATTTTAG CAGTCAAGAT 
TCGGTTTCCA GAAAGAAAGT ACTTTCTCTG AATTTAAAGC AGACTTCTAA GACAGAGGAA 
ATTAAAAATG TATTAGGAGG GTCTACCTGC TACAACTACA GTGTAAAGGA TTTACAGGAG 
ATAAGTGGCT CTGAGCTGTG CTTTCCAAGT GGGCAGAAAA TAAAATCTGC TTATCTTCCC 
CAAAGGCAAA TTCACATACC AGCTGTTTTT CAGTCTCCTG CTCATTATAA GCAGACTTTC 
ACATCTTGCC TCATAGAACA TCTAAATATA TTGCTGTTTG GGTTAGCACA AAACCTGCAG 
AAAGCTCTTT CAAAAGTTGA CATATCATTT TATACATCAT TGAAGGGAGA GAAACTGAAA 
AACGCAGAAA ATAATGTACC ATCCTGCCAT CATAGTCAAC CTGCAAAACT TGTCATGGTT 
AAAAAGGAAG GTCCAAATAA GGGTCGTCTC TTTTATACAT GTGATGGACC CAAAGCTGAT 
CGATGTAAAT TCTTTAAATG GCTTGAGGAC GTGACTCCAG GATATTCAAC ACAGGAAGGA 
GCTCGACCTG GCATGGTTTT AAGTGATATT AAGAGTATTG GCTTATATTT AAGAAGTCAA 
AAGATACCAC TTTATGAGGA ATGCCAGCTT TTGGTGAGAA AAGGATTTGA TTTTCAGAGA 
AAACAGTATG GCAAACTAAA GAAGTTTACT ACTGTAAATC CTGAGTTTTA TAATGAACCA 
AAAACCAAAC TTTATCTTAA GCTAAGTCGG AAGGAAAGAT CTTCAGCTTA TAGCAAAAAT 
GATCTTTGGG TGGTTTCAAA AACCCTAGAC TTTGAGCTGG ATACTTTTAT CGCATGTAGT 
GCTTTCTTTG GACCATCATC TATCAATGAG ATAGAAATAC TGCCTTTGAA AGGCTATTTC 
CCTTCTAATT GGCCCACTAA CATGGTTGTC CATGCGTTAT TGGTTTGTAA TGCTAGCACA 
GAACTGACTA CTTTGAAAAA CATTCAGGAC TACTTTAATC CAGCTACTCT ACCTCTAACA 
CAGTACCTGT TAACAACTGT TGTTGTGAAG ATTTCATCAA GTAATATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_017008370.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863859.1 Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu95280
Clone ID Related Accession (Same CDS sequence)	XM_017008370.1
Accession Version	XM_017008370.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein ZGRF1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGCC AAGAATTTAT TGTTCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAATTTT ATATGATGAC 
AAAGGAGCAT GTTTGGAGAG TCTGTTTCTT AAATGCCTTG AGGTGAAACC TGGAGATGAC 
TTAGAAAGTG ATCGATACTT AATCACAGTT GAAGAGGTTA AAGTTGCTGG AGCCATAGGT 
ATTGTTAAGC AGAATGTCAA TAAAGAAGCA CCAGAGTTAA ATTCAAGGAC ATTTATATCC 
TCTGGCCGAT CTCTTGGATG TCAGCCCTCT GGCTTAAAAA GGAAGTTTAC TGGTTTTCAA 
GGACCACGTC AGGTTCCAAA GAAAATGGTT ATTATGGAAA GTGGTGAATC AGCTGCATCA 
CATGAGGCTA AGAAAACTGG CCCTACTATT TTTTCTCCAT TCTGCAGCAT GCCTCCTTTG 
TTTCCTACTG TTGGCAAGAA AGATGTAAAT AATATACTGG CAGACCCTGA GAACATTGTG 
ACTTACAAGA ACAGGGAGAG AAATGCCATG GATTTTTCTT CGGTTTTTTC TCCATCCTTC 
CAGATTAACC CAGAAGTGCT GTGTGAAGAA AATTATTTTT GCTCACCTGT CAATTCTGGA 
AATAAGCTTT CAGACTCTTT ACTGACCAAT GAGCCTGTGA AAAGAGATAG TTTGGCATCT 
CACTATTCAG GAGTTTCACA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTGAAG 
TCCGAATCAT CTAGTTCATG TGAGGAACTA AATTCTGAGA TGACAGAGCA TTTTCCTCAA 
AAACAACCAC AAGGAAGTTT AAAAATTGCT ACTAAACCAA AGTACCTAAT TCAACAGGAA 
GAGTGTGCTG AGATGAAGAG CACAGAAAAT TTATACTACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TATACATTCT 
TCTACTGTAG ATGGGAATGA TACAGAAAGG AAACCCAAGG CCCAGGAAGA TGATGTAAAT 
TCTAATTTGA AAGACCTTTC ATTACAAAAA ATTATACAGT TCGTTGAAAC GTATGCTGAA 
GAGAGGAAAA AGTATAATGT AGACCAGTCA GTCGGTAATA ATGATCCATC CTGGAATCAG 
GAAGTAAAAT TAGAAATTCC TTCATTCAAT GAAAGCAGTA GCTTACAGGT TACTTGTAGC 
AGTGCAGAAA ATGATGGTAT ATTATCAGAA TCTGACATTC AAGAAGATAA TAAAATACCT 
TTTAATCAAA ATGACAAGGG GTGCATTAAA GGATCAGTTC TCATTAAAGA AAATGCTCAG 
GAGGTAAATA CATGTGGAAC ACTGGAAAAG GAGTATGAAC AATCAGAGTC ATCTCTGCCA 
GAACTGAAAC ATCTCCAAAT TGAATCTAGT AATAATTCTA GGATCTCTGA TGACATTACA 
GACATGATTT CTGAAAGTAA AATGGATAAT GAAAGTCTTA ATAGTATTCA TGAATCTCTG 
AGTAATGTAA CACAACCATT TTTGGAGGTA ACTTTTAATC TGAACAATTT TGAGACCAGT 
GACACTGAGG AGGAATCACA GGAAAGCAAC AAAATTTCCC AGGATTCAGA GAGTTGGGTA 
AAGGACATTT TGGTTAATGA TGGCAATTCA TGTTTTCAAA AGAGGTCTGA GAATACAAAC 
TGTGAAGAGA TTGAGGGTGA ACATTTGCCA TTCTTAACAT CTGTTAGTGA CAAACCTACA 
GTGACATTTC CTGTTAAAGA GACTCTGCCA TCACAGTTTT GTGATAAAAC TTATGTGGGT 
TTTGACATGG GAATATGTAA AACTGAAAAC ACAGGAAAAG AAATAGAGGA GTATAGTGAC 
ACATTAAGCA ATTTTGAATC TTTCAAGTGG ACTGATGCTG TATACGGAGA TAATAAAGAA 
GATGCTAATA AACCTATTCA AGAAGTCAGA ATTAACTATG ATTTTGCTTT ACCCCCGAAT 
AAATCTAAAG GTATAAACAT GAATTTACAT ATTCCTCATA TTCAAAACCA GATTGCTGAA 
AACAGTAATC TATTTTCAGA AGATGCTCAA CCTCAGCCTT TTATTTTGGG AAGTGACTTA 
GACAAAAATG ATGAACATGT TTTACCCTCA ACTTCTAGTA GTGACAACAG TGTCCAACTA 
TTAAATACCA ATCAGAATCA CTATGAATGT ATTGCACTTG ATAAATCAAA TACCCACATT 
TCCAATTCTT TGTTTTACCC ACTGGGAAAA AAGCATCTTA TTTCCAAAGA CACAGAAGCA 
CATATATCTG AACCTGAAGA TTTGGGAAAG ATTAGAAGTC CACCCCCTGA CCATGTTGAG 
GTTGAAACTG CCAGAGAAGG CAAACAATAC TGGAATCCTA GAAATTCTTC AGAACTTTCT 
GGATTAGTAA ATACCATTTC TATTTTAAAG TCGCTATGTG AACACAGTAC TGCTTTAGAC 
AGCTTGGAAA TATTGAAAAA GAAAAATACT GTCTTTCAGC AAGGAACTCA ACAGACGTAT 
GAGCCAGATA GCCCTCCTGA AGTGAGGAAA CCATTTATTA CAGTAGTTTC ACCAAAGTCT 
CCTCATCTGC ACAAGGATTC TCAACAGTTC TCTTCCTCGG GAAGTAAAGA AGAGACTGCT 
TTTCAAGCTG TTATTCCTAA ACAAATAGAG AGAAAAACCT GTGACCCTAA GGTGACATCA 
CCAGAAGAAA ACATCTCTAC ATTGAGCCCT GTTTCTACCT TTTCTTTGAA CTCAAGAGAT 
GAAGACTTCA TGGTAGAATT CTCTGAGACG TCCCTGAAAG CAAGAACTTT ACCTGATGAT 
CTTCATTTTC TCAACTTGGA GGGGATGAAA AAATCCCGTT CTCTGGAGAA TGAGAACCTT 
CAAAGGCTTT CATTATTAAG TAGAACCCAG GTTCCACTTA TTACTTTGCC ACGTACTGAT 
GGGCCACCTG ACTTAGACTC TCATTCGTAT ATGATCAACT CTAACACATA CGAGTCTTCT 
GGCTCCCCCA TGCTCAATTT GTGTGAAAAG TCAGCAGTTC TTTCGTTTAG CATTGAGCCT 
GAGGACCAAA ATGAAACCTT TTTCTCTGAA GAATCTAGGG AAGTGAATCC AGGGGATGTT 
TCACTTAATA ATATATCTAC TCAGAGCAAG TGGCTGAAAT ATCAAAACAC ATCCCAATGC 
AACGTGGCTA CTCCAAACAG AGTTGATAAG AGAATAACTG ATGGCTTCTT TGCTGAGGCT 
GTTTCTGGGA TGCATTTTAG AGACACAAGT GAAAGACAGA GTGATGCTGT CAATGAAAGC 
TCTTTAGACT CTGTGCATTT GCAAATGATA AAAGGCATGC TCTATCAACA GCGGCAGGAT 
TTTAGCAGTC AAGATTCGGT TTCCAGAAAG AAAGTACTTT CTCTGAATTT AAAGCAGACT 
TCTAAGACAG AGGAAATTAA AAATGTATTA GGAGGGTCTA CCTGCTACAA CTACAGTGTA 
AAGGATTTAC AGGAGATAAG TGGCTCTGAG CTGTGCTTTC CAAGTGGGCA GAAAATAAAA 
TCTGCTTATC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA CTTTCACATC TTGCCTCATA GAACATCTAA ATATATTGCT GTTTGGGTTA 
GCACAAAACC TGCAGAAAGC TCTTTCAAAA GTTGACATAT CATTTTATAC ATCATTGAAG 
GGAGAGAAAC TGAAAAACGC AGAAAATAAT GTACCATCCT GCCATCATAG TCAACCTGCA 
AAACTTGTCA TGGTTAAAAA GGAAGGTCCA AATAAGGGTC GTCTCTTTTA TACATGTGAT 
GGACCCAAAG CTGATCGATG TAAATTCTTT AAATGGCTTG AGGACGTGAC TCCAGGATAT 
TCAACACAGG AAGGAGCTCG ACCTGGCATG GTTTTAAGTG ATATTAAGAG TATTGGCTTA 
TATTTAAGAA GTCAAAAGAT ACCACTTTAT GAGGAATGCC AGCTTTTGGT GAGAAAAGGA 
TTTGATTTTC AGAGAAAACA GTATGGCAAA CTAAAGAAGT TTACTACTGT AAATCCTGAG 
TTTTATAATG AACCAAAAAC CAAACTTTAT CTTAAGCTAA GTCGGAAGGA AAGATCTTCA 
GCTTATAGCA AAAATGATCT TTGGGTGGTT TCAAAAACCC TAGACTTTGA GCTGGATACT 
TTTATCGCAT GTAGTGCTTT CTTTGGACCA TCATCTATCA ATGAGATAGA AATACTGCCT 
TTGAAAGGCT ATTTCCCTTC TAATTGGCCC ACTAACATGG TTGTCCATGC GTTATTGGTT 
TGTAATGCTA GCACAGAACT GACTACTTTG AAAAACATTC AGGACTACTT TAATCCAGCT 
ACTCTACCTC TAACACAGTA CCTGTTAACA ACGTCTTCGC CAACTATAGT TAGTAACAAA 
AGAGTCAGTA AGAGAAAATT TATCCCACCA GCCTTCACAA ATGTCAGTAC AAAATTTGAA 
CTACTCAGCC TAGGAGCAAC ATTGAAGTTA GCTAGTGAGT TGATTCAGGT ACACAAGTTA 
AACAAGGATC AAGCTACAGC TCTAATTCAA ATAGCTCAAA TGATGGCATC ACATGAAAGC 
ATTGAAGAAG TGAAGGAACT GCAAACTCAT ACCTTCCCTA TCACAATCAT ACATGGTGTG 
TTTGGAGCAG GAAAGAGTTA CTTGCTGGCA GTGGTGATTT TGTTCTTTGT ACAGCTGTTT 
GAAAAGAGTG AAGCTCCCAC CATTGGAAAT GCAAGGCCGT GGAAACTTCT GATTTCTTCT 
TCTACTAATG TGGCTGTTGA CAGAGTACTT CTTGGGCTTC TCAGTCTTGG ATTTGAAAAC 
TTTATCAGAG TTGGGAGTGT TAGGAAGATT GCCAAACCAA TTTTACCTTA TAGCTTGCAT 
GCTGGCTCAG AAAATGAAAG TGAACAGTTA AAAGAACTAC ATGCACTAAT GAAAGAAGAC 
CTGACTCCTA CGGAAAGAGT CTATGTGAGA AAAAGCATTG AGCAGCATAA ACTGGGGACC 
AATAGAACCC TGCTGAAGCA GGTTCGAGTA GTTGGAGTTA CCTGTGCAGC CTGCCCATTC 
CCATGCATGA ATGATCTTAA ATTTCCTGTA GTTGTGCTGG ATGAGTGTAG TCAGATAACT 
GAACCGGCCT CTCTCCTTCC CATTGCAAGG TTTGAGTGTG AAAAGCTGAT TCTTGTTGGG 
GATCCCAAAC AGCTACCTCC TACTATTCAG GGTTCTGATG CAGCTCATGA AAATGGATTG 
GAACAAACTC TTTTTGATCG ACTTTGCTTA ATGGGTCACA AGCCAATTCT ATTGAGAACT 
CAATACCGTT GTCATCCTGC AATCAGTGCT ATTGCTAATG ATCTGTTTTA CAAAGGAGCC 
CTCATGAATG GTGTAACAGA AATAGAGCGG AGCCCTTTAT TGGAATGGCT ACCAACCCTG 
TGTTTTTATA ATGTTAAAGG ACTAGAACAG ATAGAAAGAG ATAACAGCTT TCATAATGTG 
GCAGAAGCTA CGTTTACACT CAAGCTGATT CAATCACTGA TTGCAAGTGG AATAGCAGGC 
TCTATGATTG GTGTGATAAC ATTATACAAA TCCCAGATGT ACAAGCTTTG TCATTTACTC 
AGTGCTGTGG ACTTTCACCA TCCTGATATT AAAACTGTGC AGGTGTCCAC AGTAGATGCT 
TTTCAGGGAG CTGAAAAGGA GATCATTATT CTGTCCTGTG TAAGGACAAG ACAAGTAGGA 
TTCATTGATT CAGAAAAAAG AATGAATGTT GCATTGACTA GAGGAAAGAG GCATTTGTTG 
ATTGTGGGAA ATTTAGCCTG TTTGAGGAAA AATCAACTTT GGGGACGAGT GATCCAACAC 
TGCGAAGGAA GGGAAGATGG ATTGCAACAT GCAAACCAGT ATGAACCACA GCTGAACCAT 
CTCCTTAAAG ATTATTTTGA AAAACAAGTG GAAGAAAAAC AGAAGAAAAA GAGTGAAAAA 
GAGAAATCTA AAGATAAATC TCATTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863859.1
CDS	212..6301
Translation	MESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGACLESLFLKCLEVKPGDDLESDRYLITVEEVKVAGAIGIVKQNVNKEAPELNSRTFISSGRSLGCQPSGLKRKFTGFQGPRQVPKKMVIMESGESAASHEAKKTGPTIFSPFCSMPPLFPTVGKKDVNNILADPENIVTYKNRERNAMDFSSVFSPSFQINPEVLCEENYFCSPVNSGNKLSDSLLTNEPVKRDSLASHYSGVSQNIRSKAQILALLKSESSSSCEELNSEMTEHFPQKQPQGSLKIATKPKYLIQQEECAEMKSTENLYYQHQSENTMRNKSRWAMYLSSQSSPIHSSTVDGNDTERKPKAQEDDVNSNLKDLSLQKIIQFVETYAEERKKYNVDQSVGNNDPSWNQEVKLEIPSFNESSSLQVTCSSAENDGILSESDIQEDNKIPFNQNDKGCIKGSVLIKENAQEVNTCGTLEKEYEQSESSLPELKHLQIESSNNSRISDDITDMISESKMDNESLNSIHESLSNVTQPFLEVTFNLNNFETSDTEEESQESNKISQDSESWVKDILVNDGNSCFQKRSENTNCEEIEGEHLPFLTSVSDKPTVTFPVKETLPSQFCDKTYVGFDMGICKTENTGKEIEEYSDTLSNFESFKWTDAVYGDNKEDANKPIQEVRINYDFALPPNKSKGINMNLHIPHIQNQIAENSNLFSEDAQPQPFILGSDLDKNDEHVLPSTSSSDNSVQLLNTNQNHYECIALDKSNTHISNSLFYPLGKKHLISKDTEAHISEPEDLGKIRSPPPDHVEVETAREGKQYWNPRNSSELSGLVNTISILKSLCEHSTALDSLEILKKKNTVFQQGTQQTYEPDSPPEVRKPFITVVSPKSPHLHKDSQQFSSSGSKEETAFQAVIPKQIERKTCDPKVTSPEENISTLSPVSTFSLNSRDEDFMVEFSETSLKARTLPDDLHFLNLEGMKKSRSLENENLQRLSLLSRTQVPLITLPRTDGPPDLDSHSYMINSNTYESSGSPMLNLCEKSAVLSFSIEPEDQNETFFSEESREVNPGDVSLNNISTQSKWLKYQNTSQCNVATPNRVDKRITDGFFAEAVSGMHFRDTSERQSDAVNESSLDSVHLQMIKGMLYQQRQDFSSQDSVSRKKVLSLNLKQTSKTEEIKNVLGGSTCYNYSVKDLQEISGSELCFPSGQKIKSAYLPQRQIHIPAVFQSPAHYKQTFTSCLIEHLNILLFGLAQNLQKALSKVDISFYTSLKGEKLKNAENNVPSCHHSQPAKLVMVKKEGPNKGRLFYTCDGPKADRCKFFKWLEDVTPGYSTQEGARPGMVLSDIKSIGLYLRSQKIPLYEECQLLVRKGFDFQRKQYGKLKKFTTVNPEFYNEPKTKLYLKLSRKERSSAYSKNDLWVVSKTLDFELDTFIACSAFFGPSSINEIEILPLKGYFPSNWPTNMVVHALLVCNASTELTTLKNIQDYFNPATLPLTQYLLTTSSPTIVSNKRVSKRKFIPPAFTNVSTKFELLSLGATLKLASELIQVHKLNKDQATALIQIAQMMASHESIEEVKELQTHTFPITIIHGVFGAGKSYLLAVVILFFVQLFEKSEAPTIGNARPWKLLISSSTNVAVDRVLLGLLSLGFENFIRVGSVRKIAKPILPYSLHAGSENESEQLKELHALMKEDLTPTERVYVRKSIEQHKLGTNRTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPAISAIANDLFYKGALMNGVTEIERSPLLEWLPTLCFYNVKGLEQIERDNSFHNVAEATFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAVDFHHPDIKTVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGKRHLLIVGNLACLRKNQLWGRVIQHCEGREDGLQHANQYEPQLNHLLKDYFEKQVEEKQKKKSEKEKSKDKSHS

Target ORF information:

RefSeq Version	XM_017008370.1
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008370.1

ORF Insert Sequence:

ATGGAAAGCC AAGAATTTAT TGTTCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAATTTT ATATGATGAC 
AAAGGAGCAT GTTTGGAGAG TCTGTTTCTT AAATGCCTTG AGGTGAAACC TGGAGATGAC 
TTAGAAAGTG ATCGATACTT AATCACAGTT GAAGAGGTTA AAGTTGCTGG AGCCATAGGT 
ATTGTTAAGC AGAATGTCAA TAAAGAAGCA CCAGAGTTAA ATTCAAGGAC ATTTATATCC 
TCTGGCCGAT CTCTTGGATG TCAGCCCTCT GGCTTAAAAA GGAAGTTTAC TGGTTTTCAA 
GGACCACGTC AGGTTCCAAA GAAAATGGTT ATTATGGAAA GTGGTGAATC AGCTGCATCA 
CATGAGGCTA AGAAAACTGG CCCTACTATT TTTTCTCCAT TCTGCAGCAT GCCTCCTTTG 
TTTCCTACTG TTGGCAAGAA AGATGTAAAT AATATACTGG CAGACCCTGA GAACATTGTG 
ACTTACAAGA ACAGGGAGAG AAATGCCATG GATTTTTCTT CGGTTTTTTC TCCATCCTTC 
CAGATTAACC CAGAAGTGCT GTGTGAAGAA AATTATTTTT GCTCACCTGT CAATTCTGGA 
AATAAGCTTT CAGACTCTTT ACTGACCAAT GAGCCTGTGA AAAGAGATAG TTTGGCATCT 
CACTATTCAG GAGTTTCACA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTGAAG 
TCCGAATCAT CTAGTTCATG TGAGGAACTA AATTCTGAGA TGACAGAGCA TTTTCCTCAA 
AAACAACCAC AAGGAAGTTT AAAAATTGCT ACTAAACCAA AGTACCTAAT TCAACAGGAA 
GAGTGTGCTG AGATGAAGAG CACAGAAAAT TTATACTACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TATACATTCT 
TCTACTGTAG ATGGGAATGA TACAGAAAGG AAACCCAAGG CCCAGGAAGA TGATGTAAAT 
TCTAATTTGA AAGACCTTTC ATTACAAAAA ATTATACAGT TCGTTGAAAC GTATGCTGAA 
GAGAGGAAAA AGTATAATGT AGACCAGTCA GTCGGTAATA ATGATCCATC CTGGAATCAG 
GAAGTAAAAT TAGAAATTCC TTCATTCAAT GAAAGCAGTA GCTTACAGGT TACTTGTAGC 
AGTGCAGAAA ATGATGGTAT ATTATCAGAA TCTGACATTC AAGAAGATAA TAAAATACCT 
TTTAATCAAA ATGACAAGGG GTGCATTAAA GGATCAGTTC TCATTAAAGA AAATGCTCAG 
GAGGTAAATA CATGTGGAAC ACTGGAAAAG GAGTATGAAC AATCAGAGTC ATCTCTGCCA 
GAACTGAAAC ATCTCCAAAT TGAATCTAGT AATAATTCTA GGATCTCTGA TGACATTACA 
GACATGATTT CTGAAAGTAA AATGGATAAT GAAAGTCTTA ATAGTATTCA TGAATCTCTG 
AGTAATGTAA CACAACCATT TTTGGAGGTA ACTTTTAATC TGAACAATTT TGAGACCAGT 
GACACTGAGG AGGAATCACA GGAAAGCAAC AAAATTTCCC AGGATTCAGA GAGTTGGGTA 
AAGGACATTT TGGTTAATGA TGGCAATTCA TGTTTTCAAA AGAGGTCTGA GAATACAAAC 
TGTGAAGAGA TTGAGGGTGA ACATTTGCCA TTCTTAACAT CTGTTAGTGA CAAACCTACA 
GTGACATTTC CTGTTAAAGA GACTCTGCCA TCACAGTTTT GTGATAAAAC TTATGTGGGT 
TTTGACATGG GAATATGTAA AACTGAAAAC ACAGGAAAAG AAATAGAGGA GTATAGTGAC 
ACATTAAGCA ATTTTGAATC TTTCAAGTGG ACTGATGCTG TATACGGAGA TAATAAAGAA 
GATGCTAATA AACCTATTCA AGAAGTCAGA ATTAACTATG ATTTTGCTTT ACCCCCGAAT 
AAATCTAAAG GTATAAACAT GAATTTACAT ATTCCTCATA TTCAAAACCA GATTGCTGAA 
AACAGTAATC TATTTTCAGA AGATGCTCAA CCTCAGCCTT TTATTTTGGG AAGTGACTTA 
GACAAAAATG ATGAACATGT TTTACCCTCA ACTTCTAGTA GTGACAACAG TGTCCAACTA 
TTAAATACCA ATCAGAATCA CTATGAATGT ATTGCACTTG ATAAATCAAA TACCCACATT 
TCCAATTCTT TGTTTTACCC ACTGGGAAAA AAGCATCTTA TTTCCAAAGA CACAGAAGCA 
CATATATCTG AACCTGAAGA TTTGGGAAAG ATTAGAAGTC CACCCCCTGA CCATGTTGAG 
GTTGAAACTG CCAGAGAAGG CAAACAATAC TGGAATCCTA GAAATTCTTC AGAACTTTCT 
GGATTAGTAA ATACCATTTC TATTTTAAAG TCGCTATGTG AACACAGTAC TGCTTTAGAC 
AGCTTGGAAA TATTGAAAAA GAAAAATACT GTCTTTCAGC AAGGAACTCA ACAGACGTAT 
GAGCCAGATA GCCCTCCTGA AGTGAGGAAA CCATTTATTA CAGTAGTTTC ACCAAAGTCT 
CCTCATCTGC ACAAGGATTC TCAACAGTTC TCTTCCTCGG GAAGTAAAGA AGAGACTGCT 
TTTCAAGCTG TTATTCCTAA ACAAATAGAG AGAAAAACCT GTGACCCTAA GGTGACATCA 
CCAGAAGAAA ACATCTCTAC ATTGAGCCCT GTTTCTACCT TTTCTTTGAA CTCAAGAGAT 
GAAGACTTCA TGGTAGAATT CTCTGAGACG TCCCTGAAAG CAAGAACTTT ACCTGATGAT 
CTTCATTTTC TCAACTTGGA GGGGATGAAA AAATCCCGTT CTCTGGAGAA TGAGAACCTT 
CAAAGGCTTT CATTATTAAG TAGAACCCAG GTTCCACTTA TTACTTTGCC ACGTACTGAT 
GGGCCACCTG ACTTAGACTC TCATTCGTAT ATGATCAACT CTAACACATA CGAGTCTTCT 
GGCTCCCCCA TGCTCAATTT GTGTGAAAAG TCAGCAGTTC TTTCGTTTAG CATTGAGCCT 
GAGGACCAAA ATGAAACCTT TTTCTCTGAA GAATCTAGGG AAGTGAATCC AGGGGATGTT 
TCACTTAATA ATATATCTAC TCAGAGCAAG TGGCTGAAAT ATCAAAACAC ATCCCAATGC 
AACGTGGCTA CTCCAAACAG AGTTGATAAG AGAATAACTG ATGGCTTCTT TGCTGAGGCT 
GTTTCTGGGA TGCATTTTAG AGACACAAGT GAAAGACAGA GTGATGCTGT CAATGAAAGC 
TCTTTAGACT CTGTGCATTT GCAAATGATA AAAGGCATGC TCTATCAACA GCGGCAGGAT 
TTTAGCAGTC AAGATTCGGT TTCCAGAAAG AAAGTACTTT CTCTGAATTT AAAGCAGACT 
TCTAAGACAG AGGAAATTAA AAATGTATTA GGAGGGTCTA CCTGCTACAA CTACAGTGTA 
AAGGATTTAC AGGAGATAAG TGGCTCTGAG CTGTGCTTTC CAAGTGGGCA GAAAATAAAA 
TCTGCTTATC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA CTTTCACATC TTGCCTCATA GAACATCTAA ATATATTGCT GTTTGGGTTA 
GCACAAAACC TGCAGAAAGC TCTTTCAAAA GTTGACATAT CATTTTATAC ATCATTGAAG 
GGAGAGAAAC TGAAAAACGC AGAAAATAAT GTACCATCCT GCCATCATAG TCAACCTGCA 
AAACTTGTCA TGGTTAAAAA GGAAGGTCCA AATAAGGGTC GTCTCTTTTA TACATGTGAT 
GGACCCAAAG CTGATCGATG TAAATTCTTT AAATGGCTTG AGGACGTGAC TCCAGGATAT 
TCAACACAGG AAGGAGCTCG ACCTGGCATG GTTTTAAGTG ATATTAAGAG TATTGGCTTA 
TATTTAAGAA GTCAAAAGAT ACCACTTTAT GAGGAATGCC AGCTTTTGGT GAGAAAAGGA 
TTTGATTTTC AGAGAAAACA GTATGGCAAA CTAAAGAAGT TTACTACTGT AAATCCTGAG 
TTTTATAATG AACCAAAAAC CAAACTTTAT CTTAAGCTAA GTCGGAAGGA AAGATCTTCA 
GCTTATAGCA AAAATGATCT TTGGGTGGTT TCAAAAACCC TAGACTTTGA GCTGGATACT 
TTTATCGCAT GTAGTGCTTT CTTTGGACCA TCATCTATCA ATGAGATAGA AATACTGCCT 
TTGAAAGGCT ATTTCCCTTC TAATTGGCCC ACTAACATGG TTGTCCATGC GTTATTGGTT 
TGTAATGCTA GCACAGAACT GACTACTTTG AAAAACATTC AGGACTACTT TAATCCAGCT 
ACTCTACCTC TAACACAGTA CCTGTTAACA ACGTCTTCGC CAACTATAGT TAGTAACAAA 
AGAGTCAGTA AGAGAAAATT TATCCCACCA GCCTTCACAA ATGTCAGTAC AAAATTTGAA 
CTACTCAGCC TAGGAGCAAC ATTGAAGTTA GCTAGTGAGT TGATTCAGGT ACACAAGTTA 
AACAAGGATC AAGCTACAGC TCTAATTCAA ATAGCTCAAA TGATGGCATC ACATGAAAGC 
ATTGAAGAAG TGAAGGAACT GCAAACTCAT ACCTTCCCTA TCACAATCAT ACATGGTGTG 
TTTGGAGCAG GAAAGAGTTA CTTGCTGGCA GTGGTGATTT TGTTCTTTGT ACAGCTGTTT 
GAAAAGAGTG AAGCTCCCAC CATTGGAAAT GCAAGGCCGT GGAAACTTCT GATTTCTTCT 
TCTACTAATG TGGCTGTTGA CAGAGTACTT CTTGGGCTTC TCAGTCTTGG ATTTGAAAAC 
TTTATCAGAG TTGGGAGTGT TAGGAAGATT GCCAAACCAA TTTTACCTTA TAGCTTGCAT 
GCTGGCTCAG AAAATGAAAG TGAACAGTTA AAAGAACTAC ATGCACTAAT GAAAGAAGAC 
CTGACTCCTA CGGAAAGAGT CTATGTGAGA AAAAGCATTG AGCAGCATAA ACTGGGGACC 
AATAGAACCC TGCTGAAGCA GGTTCGAGTA GTTGGAGTTA CCTGTGCAGC CTGCCCATTC 
CCATGCATGA ATGATCTTAA ATTTCCTGTA GTTGTGCTGG ATGAGTGTAG TCAGATAACT 
GAACCGGCCT CTCTCCTTCC CATTGCAAGG TTTGAGTGTG AAAAGCTGAT TCTTGTTGGG 
GATCCCAAAC AGCTACCTCC TACTATTCAG GGTTCTGATG CAGCTCATGA AAATGGATTG 
GAACAAACTC TTTTTGATCG ACTTTGCTTA ATGGGTCACA AGCCAATTCT ATTGAGAACT 
CAATACCGTT GTCATCCTGC AATCAGTGCT ATTGCTAATG ATCTGTTTTA CAAAGGAGCC 
CTCATGAATG GTGTAACAGA AATAGAGCGG AGCCCTTTAT TGGAATGGCT ACCAACCCTG 
TGTTTTTATA ATGTTAAAGG ACTAGAACAG ATAGAAAGAG ATAACAGCTT TCATAATGTG 
GCAGAAGCTA CGTTTACACT CAAGCTGATT CAATCACTGA TTGCAAGTGG AATAGCAGGC 
TCTATGATTG GTGTGATAAC ATTATACAAA TCCCAGATGT ACAAGCTTTG TCATTTACTC 
AGTGCTGTGG ACTTTCACCA TCCTGATATT AAAACTGTGC AGGTGTCCAC AGTAGATGCT 
TTTCAGGGAG CTGAAAAGGA GATCATTATT CTGTCCTGTG TAAGGACAAG ACAAGTAGGA 
TTCATTGATT CAGAAAAAAG AATGAATGTT GCATTGACTA GAGGAAAGAG GCATTTGTTG 
ATTGTGGGAA ATTTAGCCTG TTTGAGGAAA AATCAACTTT GGGGACGAGT GATCCAACAC 
TGCGAAGGAA GGGAAGATGG ATTGCAACAT GCAAACCAGT ATGAACCACA GCTGAACCAT 
CTCCTTAAAG ATTATTTTGA AAAACAAGTG GAAGAAAAAC AGAAGAAAAA GAGTGAAAAA 
GAGAAATCTA AAGATAAATC TCATTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_017008371.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863860.1 Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X7, mRNA.

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CloneID	OHu95281
Clone ID Related Accession (Same CDS sequence)	XM_017008371.1
Accession Version	XM_017008371.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein ZGRF1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGCC AAGAATTTAT TGTTCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGTGAAACC TGGAGATGAC 
TTAGAAAGTG ATCGATACTT AATCACAGTT GAAGAGGTTA AAGTTGCTGG AGCCATAGGT 
ATTGTTAAGC AGAATGTCAA TAAAGAAGCA CCAGAGTTAA ATTCAAGGAC ATTTATATCC 
TCTGGCCGAT CTCTTGGATG TCAGCCCTCT GGCTTAAAAA GGAAGTTTAC TGGTTTTCAA 
GGACCACGTC AGGTTCCAAA GAAAATGGTT ATTATGGAAA GTGGTGAATC AGCTGCATCA 
CATGAGGCTA AGAAAACTGG CCCTACTATT TTTTCTCCAT TCTGCAGCAT GCCTCCTTTG 
TTTCCTACTG TTGGCAAGAA AGATGTAAAT AATATACTGG CAGACCCTGA GAACATTGTG 
ACTTACAAGA ACAGGGAGAG AAATGCCATG GATTTTTCTT CGGTTTTTTC TCCATCCTTC 
CAGATTAACC CAGAAGTGCT GTGTGAAGAA AATTATTTTT GCTCACCTGT CAATTCTGGA 
AATAAGCTTT CAGACTCTTT ACTGACCAAT GAGCCTGTGA AAAGAGATAG TTTGGCATCT 
CACTATTCAG GAGTTTCACA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTGAAG 
TCCGAATCAT CTAGTTCATG TGAGGAACTA AATTCTGAGA TGACAGAGCA TTTTCCTCAA 
AAACAACCAC AAGGAAGTTT AAAAATTGCT ACTAAACCAA AGTACCTAAT TCAACAGGAA 
GAGTGTGCTG AGATGAAGAG CACAGAAAAT TTATACTACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TATACATTCT 
TCTACTGTAG ATGGGAATGA TACAGAAAGG AAACCCAAGG CCCAGGAAGA TGATGTAAAT 
TCTAATTTGA AAGACCTTTC ATTACAAAAA ATTATACAGT TCGTTGAAAC GTATGCTGAA 
GAGAGGAAAA AGTATAATGT AGACCAGTCA GTCGGTAATA ATGATCCATC CTGGAATCAG 
GAAGTAAAAT TAGAAATTCC TTCATTCAAT GAAAGCAGTA GCTTACAGGT TACTTGTAGC 
AGTGCAGAAA ATGATGGTAT ATTATCAGAA TCTGACATTC AAGAAGATAA TAAAATACCT 
TTTAATCAAA ATGACAAGGG GTGCATTAAA GGATCAGTTC TCATTAAAGA AAATGCTCAG 
GAGGTAAATA CATGTGGAAC ACTGGAAAAG GAGTATGAAC AATCAGAGTC ATCTCTGCCA 
GAACTGAAAC ATCTCCAAAT TGAATCTAGT AATAATTCTA GGATCTCTGA TGACATTACA 
GACATGATTT CTGAAAGTAA AATGGATAAT GAAAGTCTTA ATAGTATTCA TGAATCTCTG 
AGTAATGTAA CACAACCATT TTTGGAGGTA ACTTTTAATC TGAACAATTT TGAGACCAGT 
GACACTGAGG AGGAATCACA GGAAAGCAAC AAAATTTCCC AGGATTCAGA GAGTTGGGTA 
AAGGACATTT TGGTTAATGA TGGCAATTCA TGTTTTCAAA AGAGGTCTGA GAATACAAAC 
TGTGAAGAGA TTGAGGGTGA ACATTTGCCA TTCTTAACAT CTGTTAGTGA CAAACCTACA 
GTGACATTTC CTGTTAAAGA GACTCTGCCA TCACAGTTTT GTGATAAAAC TTATGTGGGT 
TTTGACATGG GAATATGTAA AACTGAAAAC ACAGGAAAAG AAATAGAGGA GTATAGTGAC 
ACATTAAGCA ATTTTGAATC TTTCAAGTGG ACTGATGCTG TATACGGAGA TAATAAAGAA 
GATGCTAATA AACCTATTCA AGAAGTCAGA ATTAACTATG ATTTTGCTTT ACCCCCGAAT 
AAATCTAAAG GTATAAACAT GAATTTACAT ATTCCTCATA TTCAAAACCA GATTGCTGAA 
AACAGTAATC TATTTTCAGA AGATGCTCAA CCTCAGCCTT TTATTTTGGG AAGTGACTTA 
GACAAAAATG ATGAACATGT TTTACCCTCA ACTTCTAGTA GTGACAACAG TGTCCAACTA 
TTAAATACCA ATCAGAATCA CTATGAATGT ATTGCACTTG ATAAATCAAA TACCCACATT 
TCCAATTCTT TGTTTTACCC ACTGGGAAAA AAGCATCTTA TTTCCAAAGA CACAGAAGCA 
CATATATCTG AACCTGAAGA TTTGGGAAAG ATTAGAAGTC CACCCCCTGA CCATGTTGAG 
GTTGAAACTG CCAGAGAAGG CAAACAATAC TGGAATCCTA GAAATTCTTC AGAACTTTCT 
GGATTAGTAA ATACCATTTC TATTTTAAAG TCGCTATGTG AACACAGTAC TGCTTTAGAC 
AGCTTGGAAA TATTGAAAAA GAAAAATACT GTCTTTCAGC AAGGAACTCA ACAGACGTAT 
GAGCCAGATA GCCCTCCTGA AGTGAGGAAA CCATTTATTA CAGTAGTTTC ACCAAAGTCT 
CCTCATCTGC ACAAGGATTC TCAACAGTTC TCTTCCTCGG GAAGTAAAGA AGAGACTGCT 
TTTCAAGCTG TTATTCCTAA ACAAATAGAG AGAAAAACCT GTGACCCTAA GGTGACATCA 
CCAGAAGAAA ACATCTCTAC ATTGAGCCCT GTTTCTACCT TTTCTTTGAA CTCAAGAGAT 
GAAGACTTCA TGGTAGAATT CTCTGAGACG TCCCTGAAAG CAAGAACTTT ACCTGATGAT 
CTTCATTTTC TCAACTTGGA GGGGATGAAA AAATCCCGTT CTCTGGAGAA TGAGAACCTT 
CAAAGGCTTT CATTATTAAG TAGAACCCAG GTTCCACTTA TTACTTTGCC ACGTACTGAT 
GGGCCACCTG ACTTAGACTC TCATTCGTAT ATGATCAACT CTAACACATA CGAGTCTTCT 
GGCTCCCCCA TGCTCAATTT GTGTGAAAAG TCAGCAGTTC TTTCGTTTAG CATTGAGCCT 
GAGGACCAAA ATGAAACCTT TTTCTCTGAA GAATCTAGGG AAGTGAATCC AGGGGATGTT 
TCACTTAATA ATATATCTAC TCAGAGCAAG TGGCTGAAAT ATCAAAACAC ATCCCAATGC 
AACGTGGCTA CTCCAAACAG AGTTGATAAG AGAATAACTG ATGGCTTCTT TGCTGAGGCT 
GTTTCTGGGA TGCATTTTAG AGACACAAGT GAAAGACAGA GTGATGCTGT CAATGAAAGC 
TCTTTAGACT CTGTGCATTT GCAAATGATA AAAGGCATGC TCTATCAACA GCGGCAGGAT 
TTTAGCAGTC AAGATTCGGT TTCCAGAAAG AAAGTACTTT CTCTGAATTT AAAGCAGACT 
TCTAAGACAG AGGAAATTAA AAATGTATTA GGAGGGTCTA CCTGCTACAA CTACAGTGTA 
AAGGATTTAC AGGAGATAAG TGGCTCTGAG CTGTGCTTTC CAAGTGGGCA GAAAATAAAA 
TCTGCTTATC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA CTTTCACATC TTGCCTCATA GAACATCTAA ATATATTGCT GTTTGGGTTA 
GCACAAAACC TGCAGAAAGC TCTTTCAAAA GTTGACATAT CATTTTATAC ATCATTGAAG 
GGAGAGAAAC TGAAAAACGC AGAAAATAAT GTACCATCCT GCCATCATAG TCAACCTGCA 
AAACTTGTCA TGGTTAAAAA GGAAGGTCCA AATAAGGGTC GTCTCTTTTA TACATGTGAT 
GGACCCAAAG CTGATCGATG TAAATTCTTT AAATGGCTTG AGGACGTGAC TCCAGGATAT 
TCAACACAGG AAGGAGCTCG ACCTGGCATG GTTTTAAGTG ATATTAAGAG TATTGGCTTA 
TATTTAAGAA GTCAAAAGAT ACCACTTTAT GAGGAATGCC AGCTTTTGGT GAGAAAAGGA 
TTTGATTTTC AGAGAAAACA GTATGGCAAA CTAAAGAAGT TTACTACTGT AAATCCTGAG 
TTTTATAATG AACCAAAAAC CAAACTTTAT CTTAAGCTAA GTCGGAAGGA AAGATCTTCA 
GCTTATAGCA AAAATGATCT TTGGGTGGTT TCAAAAACCC TAGACTTTGA GCTGGATACT 
TTTATCGCAT GTAGTGCTTT CTTTGGACCA TCATCTATCA ATGAGATAGA AATACTGCCT 
TTGAAAGGCT ATTTCCCTTC TAATTGGCCC ACTAACATGG TTGTCCATGC GTTATTGGTT 
TGTAATGCTA GCACAGAACT GACTACTTTG AAAAACATTC AGGACTACTT TAATCCAGCT 
ACTCTACCTC TAACACAGTA CCTGTTAACA ACGTCTTCGC CAACTATAGT TAGTAACAAA 
AGAGTCAGTA AGAGAAAATT TATCCCACCA GCCTTCACAA ATGTCAGTAC AAAATTTGAA 
CTACTCAGCC TAGGAGCAAC ATTGAAGTTA GCTAGTGAGT TGATTCAGGT ACACAAGTTA 
AACAAGGATC AAGCTACAGC TCTAATTCAA ATAGCTCAAA TGATGGCATC ACATGAAAGC 
ATTGAAGAAG TGAAGGAACT GCAAACTCAT ACCTTCCCTA TCACAATCAT ACATGGTGTG 
TTTGGAGCAG GAAAGAGTTA CTTGCTGGCA GTGGTGATTT TGTTCTTTGT ACAGCTGTTT 
GAAAAGAGTG AAGCTCCCAC CATTGGAAAT GCAAGGCCGT GGAAACTTCT GATTTCTTCT 
TCTACTAATG TGGCTGTTGA CAGAGTACTT CTTGGGCTTC TCAGTCTTGG ATTTGAAAAC 
TTTATCAGAG TTGGGAGTGT TAGGAAGATT GCCAAACCAA TTTTACCTTA TAGCTTGCAT 
GCTGGCTCAG AAAATGAAAG TGAACAGTTA AAAGAACTAC ATGCACTAAT GAAAGAAGAC 
CTGACTCCTA CGGAAAGAGT CTATGTGAGA AAAAGCATTG AGCAGCATAA ACTGGGGACC 
AATAGAACCC TGCTGAAGCA GGTTCGAGTA GTTGGAGTTA CCTGTGCAGC CTGCCCATTC 
CCATGCATGA ATGATCTTAA ATTTCCTGTA GTTGTGCTGG ATGAGTGTAG TCAGATAACT 
GAACCGGCCT CTCTCCTTCC CATTGCAAGG TTTGAGTGTG AAAAGCTGAT TCTTGTTGGG 
GATCCCAAAC AGCTACCTCC TACTATTCAG GGTTCTGATG CAGCTCATGA AAATGGATTG 
GAACAAACTC TTTTTGATCG ACTTTGCTTA ATGGGTCACA AGCCAATTCT ATTGAGAACT 
CAATACCGTT GTCATCCTGC AATCAGTGCT ATTGCTAATG ATCTGTTTTA CAAAGGAGCC 
CTCATGAATG GTGTAACAGA AATAGAGCGG AGCCCTTTAT TGGAATGGCT ACCAACCCTG 
TGTTTTTATA ATGTTAAAGG ACTAGAACAG ATAGAAAGAG ATAACAGCTT TCATAATGTG 
GCAGAAGCTA CGTTTACACT CAAGCTGATT CAATCACTGA TTGCAAGTGG AATAGCAGGC 
TCTATGATTG GTGTGATAAC ATTATACAAA TCCCAGATGT ACAAGCTTTG TCATTTACTC 
AGTGCTGTGG ACTTTCACCA TCCTGATATT AAAACTGTGC AGGTGTCCAC AGTAGATGCT 
TTTCAGGGAG CTGAAAAGGA GATCATTATT CTGTCCTGTG TAAGGACAAG ACAAGTAGGA 
TTCATTGATT CAGAAAAAAG AATGAATGTT GCATTGACTA GAGGAAAGAG GCATTTGTTG 
ATTGTGGGAA ATTTAGCCTG TTTGAGGAAA AATCAACTTT GGGGACGAGT GATCCAACAC 
TGCGAAGGAA GGGAAGATGG ATTGCAACAT GCAAACCAGT ATGAACCACA GCTGAACCAT 
CTCCTTAAAG ATTATTTTGA AAAACAAGTG GAAGAAAAAC AGAAGAAAAA GAGTGAAAAA 
GAGAAATCTA AAGATAAATC TCATTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863860.1
CDS	152..6181
Translation	MESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKVKPGDDLESDRYLITVEEVKVAGAIGIVKQNVNKEAPELNSRTFISSGRSLGCQPSGLKRKFTGFQGPRQVPKKMVIMESGESAASHEAKKTGPTIFSPFCSMPPLFPTVGKKDVNNILADPENIVTYKNRERNAMDFSSVFSPSFQINPEVLCEENYFCSPVNSGNKLSDSLLTNEPVKRDSLASHYSGVSQNIRSKAQILALLKSESSSSCEELNSEMTEHFPQKQPQGSLKIATKPKYLIQQEECAEMKSTENLYYQHQSENTMRNKSRWAMYLSSQSSPIHSSTVDGNDTERKPKAQEDDVNSNLKDLSLQKIIQFVETYAEERKKYNVDQSVGNNDPSWNQEVKLEIPSFNESSSLQVTCSSAENDGILSESDIQEDNKIPFNQNDKGCIKGSVLIKENAQEVNTCGTLEKEYEQSESSLPELKHLQIESSNNSRISDDITDMISESKMDNESLNSIHESLSNVTQPFLEVTFNLNNFETSDTEEESQESNKISQDSESWVKDILVNDGNSCFQKRSENTNCEEIEGEHLPFLTSVSDKPTVTFPVKETLPSQFCDKTYVGFDMGICKTENTGKEIEEYSDTLSNFESFKWTDAVYGDNKEDANKPIQEVRINYDFALPPNKSKGINMNLHIPHIQNQIAENSNLFSEDAQPQPFILGSDLDKNDEHVLPSTSSSDNSVQLLNTNQNHYECIALDKSNTHISNSLFYPLGKKHLISKDTEAHISEPEDLGKIRSPPPDHVEVETAREGKQYWNPRNSSELSGLVNTISILKSLCEHSTALDSLEILKKKNTVFQQGTQQTYEPDSPPEVRKPFITVVSPKSPHLHKDSQQFSSSGSKEETAFQAVIPKQIERKTCDPKVTSPEENISTLSPVSTFSLNSRDEDFMVEFSETSLKARTLPDDLHFLNLEGMKKSRSLENENLQRLSLLSRTQVPLITLPRTDGPPDLDSHSYMINSNTYESSGSPMLNLCEKSAVLSFSIEPEDQNETFFSEESREVNPGDVSLNNISTQSKWLKYQNTSQCNVATPNRVDKRITDGFFAEAVSGMHFRDTSERQSDAVNESSLDSVHLQMIKGMLYQQRQDFSSQDSVSRKKVLSLNLKQTSKTEEIKNVLGGSTCYNYSVKDLQEISGSELCFPSGQKIKSAYLPQRQIHIPAVFQSPAHYKQTFTSCLIEHLNILLFGLAQNLQKALSKVDISFYTSLKGEKLKNAENNVPSCHHSQPAKLVMVKKEGPNKGRLFYTCDGPKADRCKFFKWLEDVTPGYSTQEGARPGMVLSDIKSIGLYLRSQKIPLYEECQLLVRKGFDFQRKQYGKLKKFTTVNPEFYNEPKTKLYLKLSRKERSSAYSKNDLWVVSKTLDFELDTFIACSAFFGPSSINEIEILPLKGYFPSNWPTNMVVHALLVCNASTELTTLKNIQDYFNPATLPLTQYLLTTSSPTIVSNKRVSKRKFIPPAFTNVSTKFELLSLGATLKLASELIQVHKLNKDQATALIQIAQMMASHESIEEVKELQTHTFPITIIHGVFGAGKSYLLAVVILFFVQLFEKSEAPTIGNARPWKLLISSSTNVAVDRVLLGLLSLGFENFIRVGSVRKIAKPILPYSLHAGSENESEQLKELHALMKEDLTPTERVYVRKSIEQHKLGTNRTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPAISAIANDLFYKGALMNGVTEIERSPLLEWLPTLCFYNVKGLEQIERDNSFHNVAEATFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAVDFHHPDIKTVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGKRHLLIVGNLACLRKNQLWGRVIQHCEGREDGLQHANQYEPQLNHLLKDYFEKQVEEKQKKKSEKEKSKDKSHS

Target ORF information:

RefSeq Version	XM_017008371.1
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008371.1

ORF Insert Sequence:

ATGGAAAGCC AAGAATTTAT TGTTCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGTGAAACC TGGAGATGAC 
TTAGAAAGTG ATCGATACTT AATCACAGTT GAAGAGGTTA AAGTTGCTGG AGCCATAGGT 
ATTGTTAAGC AGAATGTCAA TAAAGAAGCA CCAGAGTTAA ATTCAAGGAC ATTTATATCC 
TCTGGCCGAT CTCTTGGATG TCAGCCCTCT GGCTTAAAAA GGAAGTTTAC TGGTTTTCAA 
GGACCACGTC AGGTTCCAAA GAAAATGGTT ATTATGGAAA GTGGTGAATC AGCTGCATCA 
CATGAGGCTA AGAAAACTGG CCCTACTATT TTTTCTCCAT TCTGCAGCAT GCCTCCTTTG 
TTTCCTACTG TTGGCAAGAA AGATGTAAAT AATATACTGG CAGACCCTGA GAACATTGTG 
ACTTACAAGA ACAGGGAGAG AAATGCCATG GATTTTTCTT CGGTTTTTTC TCCATCCTTC 
CAGATTAACC CAGAAGTGCT GTGTGAAGAA AATTATTTTT GCTCACCTGT CAATTCTGGA 
AATAAGCTTT CAGACTCTTT ACTGACCAAT GAGCCTGTGA AAAGAGATAG TTTGGCATCT 
CACTATTCAG GAGTTTCACA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTGAAG 
TCCGAATCAT CTAGTTCATG TGAGGAACTA AATTCTGAGA TGACAGAGCA TTTTCCTCAA 
AAACAACCAC AAGGAAGTTT AAAAATTGCT ACTAAACCAA AGTACCTAAT TCAACAGGAA 
GAGTGTGCTG AGATGAAGAG CACAGAAAAT TTATACTACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TATACATTCT 
TCTACTGTAG ATGGGAATGA TACAGAAAGG AAACCCAAGG CCCAGGAAGA TGATGTAAAT 
TCTAATTTGA AAGACCTTTC ATTACAAAAA ATTATACAGT TCGTTGAAAC GTATGCTGAA 
GAGAGGAAAA AGTATAATGT AGACCAGTCA GTCGGTAATA ATGATCCATC CTGGAATCAG 
GAAGTAAAAT TAGAAATTCC TTCATTCAAT GAAAGCAGTA GCTTACAGGT TACTTGTAGC 
AGTGCAGAAA ATGATGGTAT ATTATCAGAA TCTGACATTC AAGAAGATAA TAAAATACCT 
TTTAATCAAA ATGACAAGGG GTGCATTAAA GGATCAGTTC TCATTAAAGA AAATGCTCAG 
GAGGTAAATA CATGTGGAAC ACTGGAAAAG GAGTATGAAC AATCAGAGTC ATCTCTGCCA 
GAACTGAAAC ATCTCCAAAT TGAATCTAGT AATAATTCTA GGATCTCTGA TGACATTACA 
GACATGATTT CTGAAAGTAA AATGGATAAT GAAAGTCTTA ATAGTATTCA TGAATCTCTG 
AGTAATGTAA CACAACCATT TTTGGAGGTA ACTTTTAATC TGAACAATTT TGAGACCAGT 
GACACTGAGG AGGAATCACA GGAAAGCAAC AAAATTTCCC AGGATTCAGA GAGTTGGGTA 
AAGGACATTT TGGTTAATGA TGGCAATTCA TGTTTTCAAA AGAGGTCTGA GAATACAAAC 
TGTGAAGAGA TTGAGGGTGA ACATTTGCCA TTCTTAACAT CTGTTAGTGA CAAACCTACA 
GTGACATTTC CTGTTAAAGA GACTCTGCCA TCACAGTTTT GTGATAAAAC TTATGTGGGT 
TTTGACATGG GAATATGTAA AACTGAAAAC ACAGGAAAAG AAATAGAGGA GTATAGTGAC 
ACATTAAGCA ATTTTGAATC TTTCAAGTGG ACTGATGCTG TATACGGAGA TAATAAAGAA 
GATGCTAATA AACCTATTCA AGAAGTCAGA ATTAACTATG ATTTTGCTTT ACCCCCGAAT 
AAATCTAAAG GTATAAACAT GAATTTACAT ATTCCTCATA TTCAAAACCA GATTGCTGAA 
AACAGTAATC TATTTTCAGA AGATGCTCAA CCTCAGCCTT TTATTTTGGG AAGTGACTTA 
GACAAAAATG ATGAACATGT TTTACCCTCA ACTTCTAGTA GTGACAACAG TGTCCAACTA 
TTAAATACCA ATCAGAATCA CTATGAATGT ATTGCACTTG ATAAATCAAA TACCCACATT 
TCCAATTCTT TGTTTTACCC ACTGGGAAAA AAGCATCTTA TTTCCAAAGA CACAGAAGCA 
CATATATCTG AACCTGAAGA TTTGGGAAAG ATTAGAAGTC CACCCCCTGA CCATGTTGAG 
GTTGAAACTG CCAGAGAAGG CAAACAATAC TGGAATCCTA GAAATTCTTC AGAACTTTCT 
GGATTAGTAA ATACCATTTC TATTTTAAAG TCGCTATGTG AACACAGTAC TGCTTTAGAC 
AGCTTGGAAA TATTGAAAAA GAAAAATACT GTCTTTCAGC AAGGAACTCA ACAGACGTAT 
GAGCCAGATA GCCCTCCTGA AGTGAGGAAA CCATTTATTA CAGTAGTTTC ACCAAAGTCT 
CCTCATCTGC ACAAGGATTC TCAACAGTTC TCTTCCTCGG GAAGTAAAGA AGAGACTGCT 
TTTCAAGCTG TTATTCCTAA ACAAATAGAG AGAAAAACCT GTGACCCTAA GGTGACATCA 
CCAGAAGAAA ACATCTCTAC ATTGAGCCCT GTTTCTACCT TTTCTTTGAA CTCAAGAGAT 
GAAGACTTCA TGGTAGAATT CTCTGAGACG TCCCTGAAAG CAAGAACTTT ACCTGATGAT 
CTTCATTTTC TCAACTTGGA GGGGATGAAA AAATCCCGTT CTCTGGAGAA TGAGAACCTT 
CAAAGGCTTT CATTATTAAG TAGAACCCAG GTTCCACTTA TTACTTTGCC ACGTACTGAT 
GGGCCACCTG ACTTAGACTC TCATTCGTAT ATGATCAACT CTAACACATA CGAGTCTTCT 
GGCTCCCCCA TGCTCAATTT GTGTGAAAAG TCAGCAGTTC TTTCGTTTAG CATTGAGCCT 
GAGGACCAAA ATGAAACCTT TTTCTCTGAA GAATCTAGGG AAGTGAATCC AGGGGATGTT 
TCACTTAATA ATATATCTAC TCAGAGCAAG TGGCTGAAAT ATCAAAACAC ATCCCAATGC 
AACGTGGCTA CTCCAAACAG AGTTGATAAG AGAATAACTG ATGGCTTCTT TGCTGAGGCT 
GTTTCTGGGA TGCATTTTAG AGACACAAGT GAAAGACAGA GTGATGCTGT CAATGAAAGC 
TCTTTAGACT CTGTGCATTT GCAAATGATA AAAGGCATGC TCTATCAACA GCGGCAGGAT 
TTTAGCAGTC AAGATTCGGT TTCCAGAAAG AAAGTACTTT CTCTGAATTT AAAGCAGACT 
TCTAAGACAG AGGAAATTAA AAATGTATTA GGAGGGTCTA CCTGCTACAA CTACAGTGTA 
AAGGATTTAC AGGAGATAAG TGGCTCTGAG CTGTGCTTTC CAAGTGGGCA GAAAATAAAA 
TCTGCTTATC TTCCCCAAAG GCAAATTCAC ATACCAGCTG TTTTTCAGTC TCCTGCTCAT 
TATAAGCAGA CTTTCACATC TTGCCTCATA GAACATCTAA ATATATTGCT GTTTGGGTTA 
GCACAAAACC TGCAGAAAGC TCTTTCAAAA GTTGACATAT CATTTTATAC ATCATTGAAG 
GGAGAGAAAC TGAAAAACGC AGAAAATAAT GTACCATCCT GCCATCATAG TCAACCTGCA 
AAACTTGTCA TGGTTAAAAA GGAAGGTCCA AATAAGGGTC GTCTCTTTTA TACATGTGAT 
GGACCCAAAG CTGATCGATG TAAATTCTTT AAATGGCTTG AGGACGTGAC TCCAGGATAT 
TCAACACAGG AAGGAGCTCG ACCTGGCATG GTTTTAAGTG ATATTAAGAG TATTGGCTTA 
TATTTAAGAA GTCAAAAGAT ACCACTTTAT GAGGAATGCC AGCTTTTGGT GAGAAAAGGA 
TTTGATTTTC AGAGAAAACA GTATGGCAAA CTAAAGAAGT TTACTACTGT AAATCCTGAG 
TTTTATAATG AACCAAAAAC CAAACTTTAT CTTAAGCTAA GTCGGAAGGA AAGATCTTCA 
GCTTATAGCA AAAATGATCT TTGGGTGGTT TCAAAAACCC TAGACTTTGA GCTGGATACT 
TTTATCGCAT GTAGTGCTTT CTTTGGACCA TCATCTATCA ATGAGATAGA AATACTGCCT 
TTGAAAGGCT ATTTCCCTTC TAATTGGCCC ACTAACATGG TTGTCCATGC GTTATTGGTT 
TGTAATGCTA GCACAGAACT GACTACTTTG AAAAACATTC AGGACTACTT TAATCCAGCT 
ACTCTACCTC TAACACAGTA CCTGTTAACA ACGTCTTCGC CAACTATAGT TAGTAACAAA 
AGAGTCAGTA AGAGAAAATT TATCCCACCA GCCTTCACAA ATGTCAGTAC AAAATTTGAA 
CTACTCAGCC TAGGAGCAAC ATTGAAGTTA GCTAGTGAGT TGATTCAGGT ACACAAGTTA 
AACAAGGATC AAGCTACAGC TCTAATTCAA ATAGCTCAAA TGATGGCATC ACATGAAAGC 
ATTGAAGAAG TGAAGGAACT GCAAACTCAT ACCTTCCCTA TCACAATCAT ACATGGTGTG 
TTTGGAGCAG GAAAGAGTTA CTTGCTGGCA GTGGTGATTT TGTTCTTTGT ACAGCTGTTT 
GAAAAGAGTG AAGCTCCCAC CATTGGAAAT GCAAGGCCGT GGAAACTTCT GATTTCTTCT 
TCTACTAATG TGGCTGTTGA CAGAGTACTT CTTGGGCTTC TCAGTCTTGG ATTTGAAAAC 
TTTATCAGAG TTGGGAGTGT TAGGAAGATT GCCAAACCAA TTTTACCTTA TAGCTTGCAT 
GCTGGCTCAG AAAATGAAAG TGAACAGTTA AAAGAACTAC ATGCACTAAT GAAAGAAGAC 
CTGACTCCTA CGGAAAGAGT CTATGTGAGA AAAAGCATTG AGCAGCATAA ACTGGGGACC 
AATAGAACCC TGCTGAAGCA GGTTCGAGTA GTTGGAGTTA CCTGTGCAGC CTGCCCATTC 
CCATGCATGA ATGATCTTAA ATTTCCTGTA GTTGTGCTGG ATGAGTGTAG TCAGATAACT 
GAACCGGCCT CTCTCCTTCC CATTGCAAGG TTTGAGTGTG AAAAGCTGAT TCTTGTTGGG 
GATCCCAAAC AGCTACCTCC TACTATTCAG GGTTCTGATG CAGCTCATGA AAATGGATTG 
GAACAAACTC TTTTTGATCG ACTTTGCTTA ATGGGTCACA AGCCAATTCT ATTGAGAACT 
CAATACCGTT GTCATCCTGC AATCAGTGCT ATTGCTAATG ATCTGTTTTA CAAAGGAGCC 
CTCATGAATG GTGTAACAGA AATAGAGCGG AGCCCTTTAT TGGAATGGCT ACCAACCCTG 
TGTTTTTATA ATGTTAAAGG ACTAGAACAG ATAGAAAGAG ATAACAGCTT TCATAATGTG 
GCAGAAGCTA CGTTTACACT CAAGCTGATT CAATCACTGA TTGCAAGTGG AATAGCAGGC 
TCTATGATTG GTGTGATAAC ATTATACAAA TCCCAGATGT ACAAGCTTTG TCATTTACTC 
AGTGCTGTGG ACTTTCACCA TCCTGATATT AAAACTGTGC AGGTGTCCAC AGTAGATGCT 
TTTCAGGGAG CTGAAAAGGA GATCATTATT CTGTCCTGTG TAAGGACAAG ACAAGTAGGA 
TTCATTGATT CAGAAAAAAG AATGAATGTT GCATTGACTA GAGGAAAGAG GCATTTGTTG 
ATTGTGGGAA ATTTAGCCTG TTTGAGGAAA AATCAACTTT GGGGACGAGT GATCCAACAC 
TGCGAAGGAA GGGAAGATGG ATTGCAACAT GCAAACCAGT ATGAACCACA GCTGAACCAT 
CTCCTTAAAG ATTATTTTGA AAAACAAGTG GAAGAAAAAC AGAAGAAAAA GAGTGAAAAA 
GAGAAATCTA AAGATAAATC TCATTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( NM_001350397.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001337326.1 Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant 2, mRNA.

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CloneID	OHu69373
Clone ID Related Accession (Same CDS sequence)	NM_001350397.1
Accession Version	NM_001350397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Homo sapiens(human)
Product	protein ZGRF1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC106864.5 and AC023886.7. On Apr 14, 2017 this sequence version replaced XM_011532093.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.108396.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAAAGCC AAGAATTTAT TGTTCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAATTTT ATATGATGAC 
AAAGGAGCAT GTTTGGAGAG TCTGTTTCTT AAATGCCTTG AGGTGAAACC TGGAGATGAC 
TTAGAAAGTG ATCGATACTT AATCACAGTT GAAGAGGTTA AAGTTGCTGG AGCCATAGGT 
ATTGTTAAGC AGAATGTCAA TAAAGAAGCA CCAGAGTTAA ATTCAAGGAC ATTTATATCC 
TCTGGCCGAT CTCTTGGATG TCAGCCCTCT GGCTTAAAAA GGAAGTTTAC TGGTTTTCAA 
GGACCACGTC AGGTTCCAAA GAAAATGGTT ATTATGGAAA GTGGTGAATC AGCTGCATCA 
CATGAGGCTA AGAAAACTGG CCCTACTATT TTTTCTCCAT TCTGCAGCAT GCCTCCTTTG 
TTTCCTACTG TTGGCAAGAA AGATGTAAAT AATATACTGG CAGACCCTGA GAACATTGTG 
ACTTACAAGA ACAGGGAGAG AAATGCCATG GATTTTTCTT CGGTTTTTTC TCCATCCTTC 
CAGATTAACC CAGAAGTGCT GTGTGAAGAA AATTATTTTT GCTCACCTGT CAATTCTGGA 
AATAAGCTTT CAGACTCTTT ACTGACCAAT GAGCCTGTGA AAAGAGATAG TTTGGCATCT 
CACTATTCAG GAGTTTCACA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTGAAG 
TCCGAATCAT CTAGTTCATG TGAGGAACTA AATTCTGAGA TGACAGAGCA TTTTCCTCAA 
AAACAACCAC AAGGAAGTTT AAAAATTGCT ACTAAACCAA AGTACCTAAT TCAACAGGAA 
GAGTGTGCTG AGATGAAGAG CACAGAAAAT TTATACTACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TATACATTCT 
TCTACTGTAG ATGGGAATGA TACAGAAAGG AAACCCAAGG CCCAGGAAGA TGATGTAAAT 
TCTAATTTGA AAGACCTTTC ATTACAAAAA ATTATACAGT TCGTTGAAAC GTATGCTGAA 
GAGAGGAAAA AGTATAATGT AGACCAGTCA GTCGGTAATA ATGATCCATC CTGGAATCAG 
GAAGTAAAAT TAGAAATTCC TTCATTCAAT GAAAGCAGTA GCTTACAGGT TACTTGTAGC 
AGTGCAGAAA ATGATGGTAT ATTATCAGAA TCTGACATTC AAGAAGATAA TAAAATACCT 
TTTAATCAAA ATGACAAGGG GTGCATTAAA GGATCAGTTC TCATTAAAGA AAATGCTCAG 
GAGGTAAATA CATGTGGAAC ACTGGAAAAG GAGTATGAAC AATCAGAGTC ATCTCTGCCA 
GAACTGAAAC ATCTCCAAAT TGAATCTAGT AATAATTCTA GGATCTCTGA TGACATTACA 
GACATGATTT CTGAAAGTAA AATGGATAAT GAAAGTCTTA ATAGTATTCA TGAATCTCTG 
AGTAATGTAA CACAACCATT TTTGGAGGTA ACTTTTAATC TGAACAATTT TGAGACCAGT 
GACACTGAGG AGGAATCACA GGAAAGCAAC AAAATTTCCC AGGATTCAGA GAGTTGGGTA 
AAGGACATTT TGGTTAATGA TGGCAATTCA TGTTTTCAAA AGAGGTCTGA GAATACAAAC 
TGTGAAGAGA TTGAGGGTGA ACATTTGCCA TTCTTAACAT CTGTTAGTGA CAAACCTACA 
GTGACATTTC CTGTTAAAGA GACTCTGCCA TCACAGTTTT GTGATAAAAC TTATGTGGGT 
TTTGACATGG GAATATGTAA AACTGAAAAC ACAGGAAAAG AAATAGAGGA GTATAGTGAC 
ACATTAAGCA ATTTTGAATC TTTCAAGTGG ACTGATGCTG TATACGGAGA TAATAAAGAA 
GATGCTAATA AACCTATTCA AGAAGTCAGA ATTAACTATG ATTTTGCTTT ACCCCCGAAT 
AAATCTAAAG GTATAAACAT GAATTTACAT ATTCCTCATA TTCAAAACCA GATTGCTGAA 
AACAGTAATC TATTTTCAGA AGATGCTCAA CCTCAGCCTT TTATTTTGGG AAGTGACTTA 
GACAAAAATG ATGAACATGT TTTACCCTCA ACTTCTAGTA GTGACAACAG TGTCCAACTA 
TTAAATACCA ATCAGAATCA CTATGAATGT ATTGCACTTG ATAAATCAAA TACCCACATT 
TCCAATTCTT TGTTTTACCC ACTGGGAAAA AAGCATCTTA TTTCCAAAGA CACAGAAGCA 
CATATATCTG AACCTGAAGA TTTGGGAAAG ATTAGAAGTC CACCCCCTGA CCATGTTGAG 
GTTGAAACTG CCAGAGAAGG CAAACAATAC TGGAATCCTA GAAATTCTTC AGAACTTTCT 
GGATTAGTAA ATACCATTTC TATTTTAAAG TCGCTATGTG AACACAGTAC TGCTTTAGAC 
AGCTTGGAAA TATTGAAAAA GAAAAATACT GTCTTTCAGC AAGGAACTCA ACAGACGTAT 
GAGCCAGATA GCCCTCCTGA AGTGAGGAAA CCATTTATTA CAGTAGTTTC ACCAAAGTCT 
CCTCATCTGC ACAAGGATTC TCAACAGATA CTAAAAGAAG ATGAAGTTGA ACTAAGTGAA 
CCACTTCAGT CTGTGCAGTT CTCTTCCTCG GGAAGTAAAG AAGAGACTGC TTTTCAAGCT 
GTTATTCCTA AACAAATAGA GAGAAAAACC TGTGACCCTA AGGTGACATC ACCAGAAGAA 
AACATCTCTA CATTGAGCCC TGTTTCTACC TTTTCTTTGA ACTCAAGAGA TGAAGACTTC 
ATGGTAGAAT TCTCTGAGAC GTCCCTGAAA GCAAGAACTT TACCTGATGA TCTTCATTTT 
CTCAACTTGG AGGGGATGAA AAAATCCCGT TCTCTGGAGA ATGAGAACCT TCAAAGGCTT 
TCATTATTAA GTAGAACCCA GGTTCCACTT ATTACTTTGC CACGTACTGA TGGGCCACCT 
GACTTAGACT CTCATTCGTA TATGATCAAC TCTAACACAT ACGAGTCTTC TGGCTCCCCC 
ATGCTCAATT TGTGTGAAAA GTCAGCAGTT CTTTCGTTTA GCATTGAGCC TGAGGACCAA 
AATGAAACCT TTTTCTCTGA AGAATCTAGG GAAGTGAATC CAGGGGATGT TTCACTTAAT 
AATATATCTA CTCAGAGCAA GTGGCTGAAA TATCAAAACA CATCCCAATG CAACGTGGCT 
ACTCCAAACA GAGTTGATAA GAGAATAACT GATGGCTTCT TTGCTGAGGC TGTTTCTGGG 
ATGCATTTTA GAGACACAAG TGAAAGACAG AGTGATGCTG TCAATGAAAG CTCTTTAGAC 
TCTGTGCATT TGCAAATGAT AAAAGGCATG CTCTATCAAC AGCGGCAGGA TTTTAGCAGT 
CAAGATTCGG TTTCCAGAAA GAAAGTACTT TCTCTGAATT TAAAGCAGAC TTCTAAGACA 
GAGGAAATTA AAAATGTATT AGGAGGGTCT ACCTGCTACA ACTACAGTGT AAAGGATTTA 
CAGGAGATAA GTGGCTCTGA GCTGTGCTTT CCAAGTGGGC AGAAAATAAA ATCTGCTTAT 
CTTCCCCAAA GGCAAATTCA CATACCAGCT GTTTTTCAGT CTCCTGCTCA TTATAAGCAG 
ACTTTCACAT CTTGCCTCAT AGAACATCTA AATATATTGC TGTTTGGGTT AGCACAAAAC 
CTGCAGAAAG CTCTTTCAAA AGTTGACATA TCATTTTATA CATCATTGAA GGGAGAGAAA 
CTGAAAAACG CAGAAAATAA TGTACCATCC TGCCATCATA GTCAACCTGC AAAACTTGTC 
ATGGTTAAAA AGGAAGGTCC AAATAAGGGT CGTCTCTTTT ATACATGTGA TGGACCCAAA 
GCTGATCGAT GTAAATTCTT TAAATGGCTT GAGGACGTGA CTCCAGGATA TTCAACACAG 
GAAGGAGCTC GACCTGGCAT GGTTTTAAGT GATATTAAGA GTATTGGCTT ATATTTAAGA 
AGTCAAAAGA TACCACTTTA TGAGGAATGC CAGCTTTTGG TGAGAAAAGG ATTTGATTTT 
CAGAGAAAAC AGTATGGCAA ACTAAAGAAG TTTACTACTG TAAATCCTGA GTTTTATAAT 
GAACCAAAAA CCAAACTTTA TCTTAAGCTA AGTCGGAAGG AAAGATCTTC AGCTTATAGC 
AAAAATGATC TTTGGGTGGT TTCAAAAACC CTAGACTTTG AGCTGGATAC TTTTATCGCA 
TGTAGTGCTT TCTTTGGACC ATCATCTATC AATGAGATAG AAATACTGCC TTTGAAAGGC 
TATTTCCCTT CTAATTGGCC CACTAACATG GTTGTCCATG CGTTATTGGT TTGTAATGCT 
AGCACAGAAC TGACTACTTT GAAAAACATT CAGGACTACT TTAATCCAGC TACTCTACCT 
CTAACACAGT ACCTGTTAAC AACGTCTTCG CCAACTATAG TTAGTAACAA AAGAGTCAGT 
AAGAGAAAAT TTATCCCACC AGCCTTCACA AATGTCAGTA CAAAATTTGA ACTACTCAGC 
CTAGGAGCAA CATTGAAGTT AGCTAGTGAG TTGATTCAGG TACACAAGTT AAACAAGGAT 
CAAGCTACAG CTCTAATTCA AATAGCTCAA ATGATGGCAT CACATGAAAG CATTGAAGAA 
GTGAAGGAAC TGCAAACTCA TACCTTCCCT ATCACAATCA TACATGGTGT GTTTGGAGCA 
GGAAAGAGTT ACTTGCTGGC AGTGGTGATT TTGTTCTTTG TACAGCTGTT TGAAAAGAGT 
GAAGCTCCCA CCATTGGAAA TGCAAGGCCG TGGAAACTTC TGATTTCTTC TTCTACTAAT 
GTGGCTGTTG ACAGAGTACT TCTTGGGCTT CTCAGTCTTG GATTTGAAAA CTTTATCAGA 
GTTGGGAGTG TTAGGAAGAT TGCCAAACCA ATTTTACCTT ATAGCTTGCA TGCTGGCTCA 
GAAAATGAAA GTGAACAGTT AAAAGAACTA CATGCACTAA TGAAAGAAGA CCTGACTCCT 
ACGGAAAGAG TCTATGTGAG AAAAAGCATT GAGCAGCATA AACTGGGGAC CAATAGAACC 
CTGCTGAAGC AGGTTCGAGT AGTTGGAGTT ACCTGTGCAG CCTGCCCATT CCCATGCATG 
AATGATCTTA AATTTCCTGT AGTTGTGCTG GATGAGTGTA GTCAGATAAC TGAACCGGCC 
TCTCTCCTTC CCATTGCAAG GTTTGAGTGT GAAAAGCTGA TTCTTGTTGG GGATCCCAAA 
CAGCTACCTC CTACTATTCA GGGTTCTGAT GCAGCTCATG AAAATGGATT GGAACAAACT 
CTTTTTGATC GACTTTGCTT AATGGGTCAC AAGCCAATTC TATTGAGAAC TCAATACCGT 
TGTCATCCTG CAATCAGTGC TATTGCTAAT GATCTGTTTT ACAAAGGAGC CCTCATGAAT 
GGTGTAACAG AAATAGAGCG GAGCCCTTTA TTGGAATGGC TACCAACCCT GTGTTTTTAT 
AATGTTAAAG GACTAGAACA GATAGAAAGA GATAACAGCT TTCATAATGT GGCAGAAGCT 
ACGTTTACAC TCAAGCTGAT TCAATCACTG ATTGCAAGTG GAATAGCAGG CTCTATGATT 
GGTGTGATAA CATTATACAA ATCCCAGATG TACAAGCTTT GTCATTTACT CAGTGCTGTG 
GACTTTCACC ATCCTGATAT TAAAACTGTG CAGGTGTCCA CAGTAGATGC TTTTCAGGGA 
GCTGAAAAGG AGATCATTAT TCTGTCCTGT GTAAGGACAA GACAAGTAGG ATTCATTGAT 
TCAGAAAAAA GAATGAATGT TGCATTGACT AGAGGAAAGA GGCATTTGTT GATTGTGGGA 
AATTTAGCCT GTTTGAGGAA AAATCAACTT TGGGGACGAG TGATCCAACA CTGCGAAGGA 
AGGGAAGATG GATTGCAACA TGCAAACCAG TATGAACCAC AGCTGAACCA TCTCCTTAAA 
GATTATTTTG AAAAACAAGT GGAAGAAAAA CAGAAGAAAA AGAGTGAAAA AGAGAAATCT 
AAAGATAAAT CTCATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001337326.1
CDS	212..6352
Translation	MESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGACLESLFLKCLEVKPGDDLESDRYLITVEEVKVAGAIGIVKQNVNKEAPELNSRTFISSGRSLGCQPSGLKRKFTGFQGPRQVPKKMVIMESGESAASHEAKKTGPTIFSPFCSMPPLFPTVGKKDVNNILADPENIVTYKNRERNAMDFSSVFSPSFQINPEVLCEENYFCSPVNSGNKLSDSLLTNEPVKRDSLASHYSGVSQNIRSKAQILALLKSESSSSCEELNSEMTEHFPQKQPQGSLKIATKPKYLIQQEECAEMKSTENLYYQHQSENTMRNKSRWAMYLSSQSSPIHSSTVDGNDTERKPKAQEDDVNSNLKDLSLQKIIQFVETYAEERKKYNVDQSVGNNDPSWNQEVKLEIPSFNESSSLQVTCSSAENDGILSESDIQEDNKIPFNQNDKGCIKGSVLIKENAQEVNTCGTLEKEYEQSESSLPELKHLQIESSNNSRISDDITDMISESKMDNESLNSIHESLSNVTQPFLEVTFNLNNFETSDTEEESQESNKISQDSESWVKDILVNDGNSCFQKRSENTNCEEIEGEHLPFLTSVSDKPTVTFPVKETLPSQFCDKTYVGFDMGICKTENTGKEIEEYSDTLSNFESFKWTDAVYGDNKEDANKPIQEVRINYDFALPPNKSKGINMNLHIPHIQNQIAENSNLFSEDAQPQPFILGSDLDKNDEHVLPSTSSSDNSVQLLNTNQNHYECIALDKSNTHISNSLFYPLGKKHLISKDTEAHISEPEDLGKIRSPPPDHVEVETAREGKQYWNPRNSSELSGLVNTISILKSLCEHSTALDSLEILKKKNTVFQQGTQQTYEPDSPPEVRKPFITVVSPKSPHLHKDSQQILKEDEVELSEPLQSVQFSSSGSKEETAFQAVIPKQIERKTCDPKVTSPEENISTLSPVSTFSLNSRDEDFMVEFSETSLKARTLPDDLHFLNLEGMKKSRSLENENLQRLSLLSRTQVPLITLPRTDGPPDLDSHSYMINSNTYESSGSPMLNLCEKSAVLSFSIEPEDQNETFFSEESREVNPGDVSLNNISTQSKWLKYQNTSQCNVATPNRVDKRITDGFFAEAVSGMHFRDTSERQSDAVNESSLDSVHLQMIKGMLYQQRQDFSSQDSVSRKKVLSLNLKQTSKTEEIKNVLGGSTCYNYSVKDLQEISGSELCFPSGQKIKSAYLPQRQIHIPAVFQSPAHYKQTFTSCLIEHLNILLFGLAQNLQKALSKVDISFYTSLKGEKLKNAENNVPSCHHSQPAKLVMVKKEGPNKGRLFYTCDGPKADRCKFFKWLEDVTPGYSTQEGARPGMVLSDIKSIGLYLRSQKIPLYEECQLLVRKGFDFQRKQYGKLKKFTTVNPEFYNEPKTKLYLKLSRKERSSAYSKNDLWVVSKTLDFELDTFIACSAFFGPSSINEIEILPLKGYFPSNWPTNMVVHALLVCNASTELTTLKNIQDYFNPATLPLTQYLLTTSSPTIVSNKRVSKRKFIPPAFTNVSTKFELLSLGATLKLASELIQVHKLNKDQATALIQIAQMMASHESIEEVKELQTHTFPITIIHGVFGAGKSYLLAVVILFFVQLFEKSEAPTIGNARPWKLLISSSTNVAVDRVLLGLLSLGFENFIRVGSVRKIAKPILPYSLHAGSENESEQLKELHALMKEDLTPTERVYVRKSIEQHKLGTNRTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPAISAIANDLFYKGALMNGVTEIERSPLLEWLPTLCFYNVKGLEQIERDNSFHNVAEATFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAVDFHHPDIKTVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGKRHLLIVGNLACLRKNQLWGRVIQHCEGREDGLQHANQYEPQLNHLLKDYFEKQVEEKQKKKSEKEKSKDKSHS

Target ORF information:

RefSeq Version	NM_001350397.1
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350397.1

ORF Insert Sequence:

ATGGAAAGCC AAGAATTTAT TGTTCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAATTTT ATATGATGAC 
AAAGGAGCAT GTTTGGAGAG TCTGTTTCTT AAATGCCTTG AGGTGAAACC TGGAGATGAC 
TTAGAAAGTG ATCGATACTT AATCACAGTT GAAGAGGTTA AAGTTGCTGG AGCCATAGGT 
ATTGTTAAGC AGAATGTCAA TAAAGAAGCA CCAGAGTTAA ATTCAAGGAC ATTTATATCC 
TCTGGCCGAT CTCTTGGATG TCAGCCCTCT GGCTTAAAAA GGAAGTTTAC TGGTTTTCAA 
GGACCACGTC AGGTTCCAAA GAAAATGGTT ATTATGGAAA GTGGTGAATC AGCTGCATCA 
CATGAGGCTA AGAAAACTGG CCCTACTATT TTTTCTCCAT TCTGCAGCAT GCCTCCTTTG 
TTTCCTACTG TTGGCAAGAA AGATGTAAAT AATATACTGG CAGACCCTGA GAACATTGTG 
ACTTACAAGA ACAGGGAGAG AAATGCCATG GATTTTTCTT CGGTTTTTTC TCCATCCTTC 
CAGATTAACC CAGAAGTGCT GTGTGAAGAA AATTATTTTT GCTCACCTGT CAATTCTGGA 
AATAAGCTTT CAGACTCTTT ACTGACCAAT GAGCCTGTGA AAAGAGATAG TTTGGCATCT 
CACTATTCAG GAGTTTCACA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTGAAG 
TCCGAATCAT CTAGTTCATG TGAGGAACTA AATTCTGAGA TGACAGAGCA TTTTCCTCAA 
AAACAACCAC AAGGAAGTTT AAAAATTGCT ACTAAACCAA AGTACCTAAT TCAACAGGAA 
GAGTGTGCTG AGATGAAGAG CACAGAAAAT TTATACTACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TATACATTCT 
TCTACTGTAG ATGGGAATGA TACAGAAAGG AAACCCAAGG CCCAGGAAGA TGATGTAAAT 
TCTAATTTGA AAGACCTTTC ATTACAAAAA ATTATACAGT TCGTTGAAAC GTATGCTGAA 
GAGAGGAAAA AGTATAATGT AGACCAGTCA GTCGGTAATA ATGATCCATC CTGGAATCAG 
GAAGTAAAAT TAGAAATTCC TTCATTCAAT GAAAGCAGTA GCTTACAGGT TACTTGTAGC 
AGTGCAGAAA ATGATGGTAT ATTATCAGAA TCTGACATTC AAGAAGATAA TAAAATACCT 
TTTAATCAAA ATGACAAGGG GTGCATTAAA GGATCAGTTC TCATTAAAGA AAATGCTCAG 
GAGGTAAATA CATGTGGAAC ACTGGAAAAG GAGTATGAAC AATCAGAGTC ATCTCTGCCA 
GAACTGAAAC ATCTCCAAAT TGAATCTAGT AATAATTCTA GGATCTCTGA TGACATTACA 
GACATGATTT CTGAAAGTAA AATGGATAAT GAAAGTCTTA ATAGTATTCA TGAATCTCTG 
AGTAATGTAA CACAACCATT TTTGGAGGTA ACTTTTAATC TGAACAATTT TGAGACCAGT 
GACACTGAGG AGGAATCACA GGAAAGCAAC AAAATTTCCC AGGATTCAGA GAGTTGGGTA 
AAGGACATTT TGGTTAATGA TGGCAATTCA TGTTTTCAAA AGAGGTCTGA GAATACAAAC 
TGTGAAGAGA TTGAGGGTGA ACATTTGCCA TTCTTAACAT CTGTTAGTGA CAAACCTACA 
GTGACATTTC CTGTTAAAGA GACTCTGCCA TCACAGTTTT GTGATAAAAC TTATGTGGGT 
TTTGACATGG GAATATGTAA AACTGAAAAC ACAGGAAAAG AAATAGAGGA GTATAGTGAC 
ACATTAAGCA ATTTTGAATC TTTCAAGTGG ACTGATGCTG TATACGGAGA TAATAAAGAA 
GATGCTAATA AACCTATTCA AGAAGTCAGA ATTAACTATG ATTTTGCTTT ACCCCCGAAT 
AAATCTAAAG GTATAAACAT GAATTTACAT ATTCCTCATA TTCAAAACCA GATTGCTGAA 
AACAGTAATC TATTTTCAGA AGATGCTCAA CCTCAGCCTT TTATTTTGGG AAGTGACTTA 
GACAAAAATG ATGAACATGT TTTACCCTCA ACTTCTAGTA GTGACAACAG TGTCCAACTA 
TTAAATACCA ATCAGAATCA CTATGAATGT ATTGCACTTG ATAAATCAAA TACCCACATT 
TCCAATTCTT TGTTTTACCC ACTGGGAAAA AAGCATCTTA TTTCCAAAGA CACAGAAGCA 
CATATATCTG AACCTGAAGA TTTGGGAAAG ATTAGAAGTC CACCCCCTGA CCATGTTGAG 
GTTGAAACTG CCAGAGAAGG CAAACAATAC TGGAATCCTA GAAATTCTTC AGAACTTTCT 
GGATTAGTAA ATACCATTTC TATTTTAAAG TCGCTATGTG AACACAGTAC TGCTTTAGAC 
AGCTTGGAAA TATTGAAAAA GAAAAATACT GTCTTTCAGC AAGGAACTCA ACAGACGTAT 
GAGCCAGATA GCCCTCCTGA AGTGAGGAAA CCATTTATTA CAGTAGTTTC ACCAAAGTCT 
CCTCATCTGC ACAAGGATTC TCAACAGATA CTAAAAGAAG ATGAAGTTGA ACTAAGTGAA 
CCACTTCAGT CTGTGCAGTT CTCTTCCTCG GGAAGTAAAG AAGAGACTGC TTTTCAAGCT 
GTTATTCCTA AACAAATAGA GAGAAAAACC TGTGACCCTA AGGTGACATC ACCAGAAGAA 
AACATCTCTA CATTGAGCCC TGTTTCTACC TTTTCTTTGA ACTCAAGAGA TGAAGACTTC 
ATGGTAGAAT TCTCTGAGAC GTCCCTGAAA GCAAGAACTT TACCTGATGA TCTTCATTTT 
CTCAACTTGG AGGGGATGAA AAAATCCCGT TCTCTGGAGA ATGAGAACCT TCAAAGGCTT 
TCATTATTAA GTAGAACCCA GGTTCCACTT ATTACTTTGC CACGTACTGA TGGGCCACCT 
GACTTAGACT CTCATTCGTA TATGATCAAC TCTAACACAT ACGAGTCTTC TGGCTCCCCC 
ATGCTCAATT TGTGTGAAAA GTCAGCAGTT CTTTCGTTTA GCATTGAGCC TGAGGACCAA 
AATGAAACCT TTTTCTCTGA AGAATCTAGG GAAGTGAATC CAGGGGATGT TTCACTTAAT 
AATATATCTA CTCAGAGCAA GTGGCTGAAA TATCAAAACA CATCCCAATG CAACGTGGCT 
ACTCCAAACA GAGTTGATAA GAGAATAACT GATGGCTTCT TTGCTGAGGC TGTTTCTGGG 
ATGCATTTTA GAGACACAAG TGAAAGACAG AGTGATGCTG TCAATGAAAG CTCTTTAGAC 
TCTGTGCATT TGCAAATGAT AAAAGGCATG CTCTATCAAC AGCGGCAGGA TTTTAGCAGT 
CAAGATTCGG TTTCCAGAAA GAAAGTACTT TCTCTGAATT TAAAGCAGAC TTCTAAGACA 
GAGGAAATTA AAAATGTATT AGGAGGGTCT ACCTGCTACA ACTACAGTGT AAAGGATTTA 
CAGGAGATAA GTGGCTCTGA GCTGTGCTTT CCAAGTGGGC AGAAAATAAA ATCTGCTTAT 
CTTCCCCAAA GGCAAATTCA CATACCAGCT GTTTTTCAGT CTCCTGCTCA TTATAAGCAG 
ACTTTCACAT CTTGCCTCAT AGAACATCTA AATATATTGC TGTTTGGGTT AGCACAAAAC 
CTGCAGAAAG CTCTTTCAAA AGTTGACATA TCATTTTATA CATCATTGAA GGGAGAGAAA 
CTGAAAAACG CAGAAAATAA TGTACCATCC TGCCATCATA GTCAACCTGC AAAACTTGTC 
ATGGTTAAAA AGGAAGGTCC AAATAAGGGT CGTCTCTTTT ATACATGTGA TGGACCCAAA 
GCTGATCGAT GTAAATTCTT TAAATGGCTT GAGGACGTGA CTCCAGGATA TTCAACACAG 
GAAGGAGCTC GACCTGGCAT GGTTTTAAGT GATATTAAGA GTATTGGCTT ATATTTAAGA 
AGTCAAAAGA TACCACTTTA TGAGGAATGC CAGCTTTTGG TGAGAAAAGG ATTTGATTTT 
CAGAGAAAAC AGTATGGCAA ACTAAAGAAG TTTACTACTG TAAATCCTGA GTTTTATAAT 
GAACCAAAAA CCAAACTTTA TCTTAAGCTA AGTCGGAAGG AAAGATCTTC AGCTTATAGC 
AAAAATGATC TTTGGGTGGT TTCAAAAACC CTAGACTTTG AGCTGGATAC TTTTATCGCA 
TGTAGTGCTT TCTTTGGACC ATCATCTATC AATGAGATAG AAATACTGCC TTTGAAAGGC 
TATTTCCCTT CTAATTGGCC CACTAACATG GTTGTCCATG CGTTATTGGT TTGTAATGCT 
AGCACAGAAC TGACTACTTT GAAAAACATT CAGGACTACT TTAATCCAGC TACTCTACCT 
CTAACACAGT ACCTGTTAAC AACGTCTTCG CCAACTATAG TTAGTAACAA AAGAGTCAGT 
AAGAGAAAAT TTATCCCACC AGCCTTCACA AATGTCAGTA CAAAATTTGA ACTACTCAGC 
CTAGGAGCAA CATTGAAGTT AGCTAGTGAG TTGATTCAGG TACACAAGTT AAACAAGGAT 
CAAGCTACAG CTCTAATTCA AATAGCTCAA ATGATGGCAT CACATGAAAG CATTGAAGAA 
GTGAAGGAAC TGCAAACTCA TACCTTCCCT ATCACAATCA TACATGGTGT GTTTGGAGCA 
GGAAAGAGTT ACTTGCTGGC AGTGGTGATT TTGTTCTTTG TACAGCTGTT TGAAAAGAGT 
GAAGCTCCCA CCATTGGAAA TGCAAGGCCG TGGAAACTTC TGATTTCTTC TTCTACTAAT 
GTGGCTGTTG ACAGAGTACT TCTTGGGCTT CTCAGTCTTG GATTTGAAAA CTTTATCAGA 
GTTGGGAGTG TTAGGAAGAT TGCCAAACCA ATTTTACCTT ATAGCTTGCA TGCTGGCTCA 
GAAAATGAAA GTGAACAGTT AAAAGAACTA CATGCACTAA TGAAAGAAGA CCTGACTCCT 
ACGGAAAGAG TCTATGTGAG AAAAAGCATT GAGCAGCATA AACTGGGGAC CAATAGAACC 
CTGCTGAAGC AGGTTCGAGT AGTTGGAGTT ACCTGTGCAG CCTGCCCATT CCCATGCATG 
AATGATCTTA AATTTCCTGT AGTTGTGCTG GATGAGTGTA GTCAGATAAC TGAACCGGCC 
TCTCTCCTTC CCATTGCAAG GTTTGAGTGT GAAAAGCTGA TTCTTGTTGG GGATCCCAAA 
CAGCTACCTC CTACTATTCA GGGTTCTGAT GCAGCTCATG AAAATGGATT GGAACAAACT 
CTTTTTGATC GACTTTGCTT AATGGGTCAC AAGCCAATTC TATTGAGAAC TCAATACCGT 
TGTCATCCTG CAATCAGTGC TATTGCTAAT GATCTGTTTT ACAAAGGAGC CCTCATGAAT 
GGTGTAACAG AAATAGAGCG GAGCCCTTTA TTGGAATGGC TACCAACCCT GTGTTTTTAT 
AATGTTAAAG GACTAGAACA GATAGAAAGA GATAACAGCT TTCATAATGT GGCAGAAGCT 
ACGTTTACAC TCAAGCTGAT TCAATCACTG ATTGCAAGTG GAATAGCAGG CTCTATGATT 
GGTGTGATAA CATTATACAA ATCCCAGATG TACAAGCTTT GTCATTTACT CAGTGCTGTG 
GACTTTCACC ATCCTGATAT TAAAACTGTG CAGGTGTCCA CAGTAGATGC TTTTCAGGGA 
GCTGAAAAGG AGATCATTAT TCTGTCCTGT GTAAGGACAA GACAAGTAGG ATTCATTGAT 
TCAGAAAAAA GAATGAATGT TGCATTGACT AGAGGAAAGA GGCATTTGTT GATTGTGGGA 
AATTTAGCCT GTTTGAGGAA AAATCAACTT TGGGGACGAG TGATCCAACA CTGCGAAGGA 
AGGGAAGATG GATTGCAACA TGCAAACCAG TATGAACCAC AGCTGAACCA TCTCCTTAAA 
GATTATTTTG AAAAACAAGT GGAAGAAAAA CAGAAGAAAA AGAGTGAAAA AGAGAAATCT 
AAAGATAAAT CTCATTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_011532091.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530393.1 Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X2, mRNA.

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CloneID	OHu69371
Clone ID Related Accession (Same CDS sequence)	XM_011532091.2
Accession Version	XM_011532091.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein ZGRF1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532091.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGCC AAGAATTTAT TGTTCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAATTTT ATATGATGAC 
AAAGGAGCAT GTTTGGAGAG TCTGTTTCTT AAATGCCTTG AGGTGAAACC TGGAGATGAC 
TTAGAAAGTG ATCGATACTT AATCACAGTT GAAGAGGTTA AAGTTGCTGG AGCCATAGGT 
ATTGTTAAGC AGAATGTCAA TAAAGAAGCA CCAGAGTTAA ATTCAAGGAC ATTTATATCC 
TCTGGCCGAT CTCTTGGATG TCAGCCCTCT GGCTTAAAAA GGAAGTTTAC TGGTTTTCAA 
GGACCACGTC AGGTTCCAAA GAAAATGGTT ATTATGGAAA GTGGTGAATC AGCTGCATCA 
CATGAGGCTA AGAAAACTGG CCCTACTATT TTTTCTCCAT TCTGCAGCAT GCCTCCTTTG 
TTTCCTACTG TTGGCAAGAA AGATGTAAAT AATATACTGG CAGACCCTGA GAACATTGTG 
ACTTACAAGA ACAGGGAGAG AAATGCCATG GATTTTTCTT CGGTTTTTTC TCCATCCTTC 
CAGATTAACC CAGAAGTGCT GTGTGAAGAA AATTATTTTT GCTCACCTGT CAATTCTGGA 
AATAAGCTTT CAGACTCTTT ACTGACCAAT GAGCCTGTGA AAAGAGATAG TTTGGCATCT 
CACTATTCAG GAGTTTCACA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTGAAG 
TCCGAATCAT CTAGTTCATG TGAGGAACTA AATTCTGAGA TGACAGAGCA TTTTCCTCAA 
AAACAACCAC AAGGAAGTTT AAAAATTGCT ACTAAACCAA AGTACCTAAT TCAACAGGAA 
GAGTGTGCTG AGATGAAGAG CACAGAAAAT TTATACTACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TATACATTCT 
TCTACTGTAG ATGGGAATGA TACAGAAAGG AAACCCAAGG CCCAGGAAGA TGATGTAAAT 
TCTAATTTGA AAGACCTTTC ATTACAAAAA ATTATACAGT TCGTTGAAAC GTATGCTGAA 
GAGAGGAAAA AGTATAATGT AGACCAGTCA GTCGGTAATA ATGATCCATC CTGGAATCAG 
GAAGTAAAAT TAGAAATTCC TTCATTCAAT GAAAGCAGTA GCTTACAGGT TACTTGTAGC 
AGTGCAGAAA ATGATGGTAT ATTATCAGAA TCTGACATTC AAGAAGATAA TAAAATACCT 
TTTAATCAAA ATGACAAGGG GTGCATTAAA GGATCAGTTC TCATTAAAGA AAATGCTCAG 
GAGGTAAATA CATGTGGAAC ACTGGAAAAG GAGTATGAAC AATCAGAGTC ATCTCTGCCA 
GAACTGAAAC ATCTCCAAAT TGAATCTAGT AATAATTCTA GGATCTCTGA TGACATTACA 
GACATGATTT CTGAAAGTAA AATGGATAAT GAAAGTCTTA ATAGTATTCA TGAATCTCTG 
AGTAATGTAA CACAACCATT TTTGGAGGTA ACTTTTAATC TGAACAATTT TGAGACCAGT 
GACACTGAGG AGGAATCACA GGAAAGCAAC AAAATTTCCC AGGATTCAGA GAGTTGGGTA 
AAGGACATTT TGGTTAATGA TGGCAATTCA TGTTTTCAAA AGAGGTCTGA GAATACAAAC 
TGTGAAGAGA TTGAGGGTGA ACATTTGCCA TTCTTAACAT CTGTTAGTGA CAAACCTACA 
GTGACATTTC CTGTTAAAGA GACTCTGCCA TCACAGTTTT GTGATAAAAC TTATGTGGGT 
TTTGACATGG GAATATGTAA AACTGAAAAC ACAGGAAAAG AAATAGAGGA GTATAGTGAC 
ACATTAAGCA ATTTTGAATC TTTCAAGTGG ACTGATGCTG TATACGGAGA TAATAAAGAA 
GATGCTAATA AACCTATTCA AGAAGTCAGA ATTAACTATG ATTTTGCTTT ACCCCCGAAT 
AAATCTAAAG GTATAAACAT GAATTTACAT ATTCCTCATA TTCAAAACCA GATTGCTGAA 
AACAGTAATC TATTTTCAGA AGATGCTCAA CCTCAGCCTT TTATTTTGGG AAGTGACTTA 
GACAAAAATG ATGAACATGT TTTACCCTCA ACTTCTAGTA GTGACAACAG TGTCCAACTA 
TTAAATACCA ATCAGAATCA CTATGAATGT ATTGCACTTG ATAAATCAAA TACCCACATT 
TCCAATTCTT TGTTTTACCC ACTGGGAAAA AAGCATCTTA TTTCCAAAGA CACAGAAGCA 
CATATATCTG AACCTGAAGA TTTGGGAAAG ATTAGAAGTC CACCCCCTGA CCATGTTGAG 
GTTGAAACTG CCAGAGAAGG CAAACAATAC TGGAATCCTA GAAATTCTTC AGAACTTTCT 
GGATTAGTAA ATACCATTTC TATTTTAAAG TCGCTATGTG AACACAGTAC TGCTTTAGAC 
AGCTTGGAAA TATTGAAAAA GAAAAATACT GTCTTTCAGC AAGGAACTCA ACAGACGTAT 
GAGCCAGATA GCCCTCCTGA AGTGAGGAAA CCATTTATTA CAGTAGTTTC ACCAAAGTCT 
CCTCATCTGC ACAAGGATTC TCAACAGTTC TCTTCCTCGG GAAGTAAAGA AGAGACTGCT 
TTTCAAGCTG TTATTCCTAA ACAAATAGAG AGAAAAACCT GTGACCCTAA GCCTGTTGAG 
TTTCAAGGAC ATCAAGTAAA AGGATCAGCT ACTAGTGGTG TAATGGTCAG AGGACACAGC 
TCACAGCTAG GATGCAGTCA GTTTCCAGAT AGCACTGAGT ATGAAAACTT TATGACAGAG 
ACACCAGAGT TACCTAGCAC GTGTATGCAG ATTGACTTCT TGCAGGTGAC ATCACCAGAA 
GAAAACATCT CTACATTGAG CCCTGTTTCT ACCTTTTCTT TGAACTCAAG AGATGAAGAC 
TTCATGGTAG AATTCTCTGA GACGTCCCTG AAAGCAAGAA CTTTACCTGA TGATCTTCAT 
TTTCTCAACT TGGAGGGGAT GAAAAAATCC CGTTCTCTGG AGAATGAGAA CCTTCAAAGG 
CTTTCATTAT TAAGTAGAAC CCAGGTTCCA CTTATTACTT TGCCACGTAC TGATGGGCCA 
CCTGACTTAG ACTCTCATTC GTATATGATC AACTCTAACA CATACGAGTC TTCTGGCTCC 
CCCATGCTCA ATTTGTGTGA AAAGTCAGCA GTTCTTTCGT TTAGCATTGA GCCTGAGGAC 
CAAAATGAAA CCTTTTTCTC TGAAGAATCT AGGGAAGTGA ATCCAGGGGA TGTTTCACTT 
AATAATATAT CTACTCAGAG CAAGTGGCTG AAATATCAAA ACACATCCCA ATGCAACGTG 
GCTACTCCAA ACAGAGTTGA TAAGAGAATA ACTGATGGCT TCTTTGCTGA GGCTGTTTCT 
GGGATGCATT TTAGAGACAC AAGTGAAAGA CAGAGTGATG CTGTCAATGA AAGCTCTTTA 
GACTCTGTGC ATTTGCAAAT GATAAAAGGC ATGCTCTATC AACAGCGGCA GGATTTTAGC 
AGTCAAGATT CGGTTTCCAG AAAGAAAGTA CTTTCTCTGA ATTTAAAGCA GACTTCTAAG 
ACAGAGGAAA TTAAAAATGT ATTAGGAGGG TCTACCTGCT ACAACTACAG TGTAAAGGAT 
TTACAGGAGA TAAGTGGCTC TGAGCTGTGC TTTCCAAGTG GGCAGAAAAT AAAATCTGCT 
TATCTTCCCC AAAGGCAAAT TCACATACCA GCTGTTTTTC AGTCTCCTGC TCATTATAAG 
CAGACTTTCA CATCTTGCCT CATAGAACAT CTAAATATAT TGCTGTTTGG GTTAGCACAA 
AACCTGCAGA AAGCTCTTTC AAAAGTTGAC ATATCATTTT ATACATCATT GAAGGGAGAG 
AAACTGAAAA ACGCAGAAAA TAATGTACCA TCCTGCCATC ATAGTCAACC TGCAAAACTT 
GTCATGGTTA AAAAGGAAGG TCCAAATAAG GGTCGTCTCT TTTATACATG TGATGGACCC 
AAAGCTGATC GATGTAAATT CTTTAAATGG CTTGAGGACG TGACTCCAGG ATATTCAACA 
CAGGAAGGAG CTCGACCTGG CATGGTTTTA AGTGATATTA AGAGTATTGG CTTATATTTA 
AGAAGTCAAA AGATACCACT TTATGAGGAA TGCCAGCTTT TGGTGAGAAA AGGATTTGAT 
TTTCAGAGAA AACAGTATGG CAAACTAAAG AAGTTTACTA CTGTAAATCC TGAGTTTTAT 
AATGAACCAA AAACCAAACT TTATCTTAAG CTAAGTCGGA AGGAAAGATC TTCAGCTTAT 
AGCAAAAATG ATCTTTGGGT GGTTTCAAAA ACCCTAGACT TTGAGCTGGA TACTTTTATC 
GCATGTAGTG CTTTCTTTGG ACCATCATCT ATCAATGAGA TAGAAATACT GCCTTTGAAA 
GGCTATTTCC CTTCTAATTG GCCCACTAAC ATGGTTGTCC ATGCGTTATT GGTTTGTAAT 
GCTAGCACAG AACTGACTAC TTTGAAAAAC ATTCAGGACT ACTTTAATCC AGCTACTCTA 
CCTCTAACAC AGTACCTGTT AACAACGTCT TCGCCAACTA TAGTTAGTAA CAAAAGAGTC 
AGTAAGAGAA AATTTATCCC ACCAGCCTTC ACAAATGTCA GTACAAAATT TGAACTACTC 
AGCCTAGGAG CAACATTGAA GTTAGCTAGT GAGTTGATTC AGGTACACAA GTTAAACAAG 
GATCAAGCTA CAGCTCTAAT TCAAATAGCT CAAATGATGG CATCACATGA AAGCATTGAA 
GAAGTGAAGG AACTGCAAAC TCATACCTTC CCTATCACAA TCATACATGG TGTGTTTGGA 
GCAGGAAAGA GTTACTTGCT GGCAGTGGTG ATTTTGTTCT TTGTACAGCT GTTTGAAAAG 
AGTGAAGCTC CCACCATTGG AAATGCAAGG CCGTGGAAAC TTCTGATTTC TTCTTCTACT 
AATGTGGCTG TTGACAGAGT ACTTCTTGGG CTTCTCAGTC TTGGATTTGA AAACTTTATC 
AGAGTTGGGA GTGTTAGGAA GATTGCCAAA CCAATTTTAC CTTATAGCTT GCATGCTGGC 
TCAGAAAATG AAAGTGAACA GTTAAAAGAA CTACATGCAC TAATGAAAGA AGACCTGACT 
CCTACGGAAA GAGTCTATGT GAGAAAAAGC ATTGAGCAGC ATAAACTGGG GACCAATAGA 
ACCCTGCTGA AGCAGGTTCG AGTAGTTGGA GTTACCTGTG CAGCCTGCCC ATTCCCATGC 
ATGAATGATC TTAAATTTCC TGTAGTTGTG CTGGATGAGT GTAGTCAGAT AACTGAACCG 
GCCTCTCTCC TTCCCATTGC AAGGTTTGAG TGTGAAAAGC TGATTCTTGT TGGGGATCCC 
AAACAGCTAC CTCCTACTAT TCAGGGTTCT GATGCAGCTC ATGAAAATGG ATTGGAACAA 
ACTCTTTTTG ATCGACTTTG CTTAATGGGT CACAAGCCAA TTCTATTGAG AACTCAATAC 
CGTTGTCATC CTGCAATCAG TGCTATTGCT AATGATCTGT TTTACAAAGG AGCCCTCATG 
AATGGTGTAA CAGAAATAGA GCGGAGCCCT TTATTGGAAT GGCTACCAAC CCTGTGTTTT 
TATAATGTTA AAGGACTAGA ACAGATAGAA AGAGATAACA GCTTTCATAA TGTGGCAGAA 
GCTACGTTTA CACTCAAGCT GATTCAATCA CTGATTGCAA GTGGAATAGC AGGCTCTATG 
ATTGGTGTGA TAACATTATA CAAATCCCAG ATGTACAAGC TTTGTCATTT ACTCAGTGCT 
GTGGACTTTC ACCATCCTGA TATTAAAACT GTGCAGGTGT CCACAGTAGA TGCTTTTCAG 
GGAGCTGAAA AGGAGATCAT TATTCTGTCC TGTGTAAGGA CAAGACAAGT AGGATTCATT 
GATTCAGAAA AAAGAATGAA TGTTGCATTG ACTAGAGGAA AGAGGCATTT GTTGATTGTG 
GGAAATTTAG CCTGTTTGAG GAAAAATCAA CTTTGGGGAC GAGTGATCCA ACACTGCGAA 
GGAAGGGAAG ATGGATTGCA ACATGCAAAC CAGTATGAAC CACAGCTGAA CCATCTCCTT 
AAAGATTATT TTGAAAAACA AGTGGAAGAA AAACAGAAGA AAAAGAGTGA AAAAGAGAAA 
TCTAAAGATA AATCTCATTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530393.1
CDS	212..6475
Translation	MESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGACLESLFLKCLEVKPGDDLESDRYLITVEEVKVAGAIGIVKQNVNKEAPELNSRTFISSGRSLGCQPSGLKRKFTGFQGPRQVPKKMVIMESGESAASHEAKKTGPTIFSPFCSMPPLFPTVGKKDVNNILADPENIVTYKNRERNAMDFSSVFSPSFQINPEVLCEENYFCSPVNSGNKLSDSLLTNEPVKRDSLASHYSGVSQNIRSKAQILALLKSESSSSCEELNSEMTEHFPQKQPQGSLKIATKPKYLIQQEECAEMKSTENLYYQHQSENTMRNKSRWAMYLSSQSSPIHSSTVDGNDTERKPKAQEDDVNSNLKDLSLQKIIQFVETYAEERKKYNVDQSVGNNDPSWNQEVKLEIPSFNESSSLQVTCSSAENDGILSESDIQEDNKIPFNQNDKGCIKGSVLIKENAQEVNTCGTLEKEYEQSESSLPELKHLQIESSNNSRISDDITDMISESKMDNESLNSIHESLSNVTQPFLEVTFNLNNFETSDTEEESQESNKISQDSESWVKDILVNDGNSCFQKRSENTNCEEIEGEHLPFLTSVSDKPTVTFPVKETLPSQFCDKTYVGFDMGICKTENTGKEIEEYSDTLSNFESFKWTDAVYGDNKEDANKPIQEVRINYDFALPPNKSKGINMNLHIPHIQNQIAENSNLFSEDAQPQPFILGSDLDKNDEHVLPSTSSSDNSVQLLNTNQNHYECIALDKSNTHISNSLFYPLGKKHLISKDTEAHISEPEDLGKIRSPPPDHVEVETAREGKQYWNPRNSSELSGLVNTISILKSLCEHSTALDSLEILKKKNTVFQQGTQQTYEPDSPPEVRKPFITVVSPKSPHLHKDSQQFSSSGSKEETAFQAVIPKQIERKTCDPKPVEFQGHQVKGSATSGVMVRGHSSQLGCSQFPDSTEYENFMTETPELPSTCMQIDFLQVTSPEENISTLSPVSTFSLNSRDEDFMVEFSETSLKARTLPDDLHFLNLEGMKKSRSLENENLQRLSLLSRTQVPLITLPRTDGPPDLDSHSYMINSNTYESSGSPMLNLCEKSAVLSFSIEPEDQNETFFSEESREVNPGDVSLNNISTQSKWLKYQNTSQCNVATPNRVDKRITDGFFAEAVSGMHFRDTSERQSDAVNESSLDSVHLQMIKGMLYQQRQDFSSQDSVSRKKVLSLNLKQTSKTEEIKNVLGGSTCYNYSVKDLQEISGSELCFPSGQKIKSAYLPQRQIHIPAVFQSPAHYKQTFTSCLIEHLNILLFGLAQNLQKALSKVDISFYTSLKGEKLKNAENNVPSCHHSQPAKLVMVKKEGPNKGRLFYTCDGPKADRCKFFKWLEDVTPGYSTQEGARPGMVLSDIKSIGLYLRSQKIPLYEECQLLVRKGFDFQRKQYGKLKKFTTVNPEFYNEPKTKLYLKLSRKERSSAYSKNDLWVVSKTLDFELDTFIACSAFFGPSSINEIEILPLKGYFPSNWPTNMVVHALLVCNASTELTTLKNIQDYFNPATLPLTQYLLTTSSPTIVSNKRVSKRKFIPPAFTNVSTKFELLSLGATLKLASELIQVHKLNKDQATALIQIAQMMASHESIEEVKELQTHTFPITIIHGVFGAGKSYLLAVVILFFVQLFEKSEAPTIGNARPWKLLISSSTNVAVDRVLLGLLSLGFENFIRVGSVRKIAKPILPYSLHAGSENESEQLKELHALMKEDLTPTERVYVRKSIEQHKLGTNRTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPAISAIANDLFYKGALMNGVTEIERSPLLEWLPTLCFYNVKGLEQIERDNSFHNVAEATFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAVDFHHPDIKTVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGKRHLLIVGNLACLRKNQLWGRVIQHCEGREDGLQHANQYEPQLNHLLKDYFEKQVEEKQKKKSEKEKSKDKSHS

Target ORF information:

RefSeq Version	XM_011532091.2
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532091.2

ORF Insert Sequence:

ATGGAAAGCC AAGAATTTAT TGTTCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAATTTT ATATGATGAC 
AAAGGAGCAT GTTTGGAGAG TCTGTTTCTT AAATGCCTTG AGGTGAAACC TGGAGATGAC 
TTAGAAAGTG ATCGATACTT AATCACAGTT GAAGAGGTTA AAGTTGCTGG AGCCATAGGT 
ATTGTTAAGC AGAATGTCAA TAAAGAAGCA CCAGAGTTAA ATTCAAGGAC ATTTATATCC 
TCTGGCCGAT CTCTTGGATG TCAGCCCTCT GGCTTAAAAA GGAAGTTTAC TGGTTTTCAA 
GGACCACGTC AGGTTCCAAA GAAAATGGTT ATTATGGAAA GTGGTGAATC AGCTGCATCA 
CATGAGGCTA AGAAAACTGG CCCTACTATT TTTTCTCCAT TCTGCAGCAT GCCTCCTTTG 
TTTCCTACTG TTGGCAAGAA AGATGTAAAT AATATACTGG CAGACCCTGA GAACATTGTG 
ACTTACAAGA ACAGGGAGAG AAATGCCATG GATTTTTCTT CGGTTTTTTC TCCATCCTTC 
CAGATTAACC CAGAAGTGCT GTGTGAAGAA AATTATTTTT GCTCACCTGT CAATTCTGGA 
AATAAGCTTT CAGACTCTTT ACTGACCAAT GAGCCTGTGA AAAGAGATAG TTTGGCATCT 
CACTATTCAG GAGTTTCACA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTGAAG 
TCCGAATCAT CTAGTTCATG TGAGGAACTA AATTCTGAGA TGACAGAGCA TTTTCCTCAA 
AAACAACCAC AAGGAAGTTT AAAAATTGCT ACTAAACCAA AGTACCTAAT TCAACAGGAA 
GAGTGTGCTG AGATGAAGAG CACAGAAAAT TTATACTACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TATACATTCT 
TCTACTGTAG ATGGGAATGA TACAGAAAGG AAACCCAAGG CCCAGGAAGA TGATGTAAAT 
TCTAATTTGA AAGACCTTTC ATTACAAAAA ATTATACAGT TCGTTGAAAC GTATGCTGAA 
GAGAGGAAAA AGTATAATGT AGACCAGTCA GTCGGTAATA ATGATCCATC CTGGAATCAG 
GAAGTAAAAT TAGAAATTCC TTCATTCAAT GAAAGCAGTA GCTTACAGGT TACTTGTAGC 
AGTGCAGAAA ATGATGGTAT ATTATCAGAA TCTGACATTC AAGAAGATAA TAAAATACCT 
TTTAATCAAA ATGACAAGGG GTGCATTAAA GGATCAGTTC TCATTAAAGA AAATGCTCAG 
GAGGTAAATA CATGTGGAAC ACTGGAAAAG GAGTATGAAC AATCAGAGTC ATCTCTGCCA 
GAACTGAAAC ATCTCCAAAT TGAATCTAGT AATAATTCTA GGATCTCTGA TGACATTACA 
GACATGATTT CTGAAAGTAA AATGGATAAT GAAAGTCTTA ATAGTATTCA TGAATCTCTG 
AGTAATGTAA CACAACCATT TTTGGAGGTA ACTTTTAATC TGAACAATTT TGAGACCAGT 
GACACTGAGG AGGAATCACA GGAAAGCAAC AAAATTTCCC AGGATTCAGA GAGTTGGGTA 
AAGGACATTT TGGTTAATGA TGGCAATTCA TGTTTTCAAA AGAGGTCTGA GAATACAAAC 
TGTGAAGAGA TTGAGGGTGA ACATTTGCCA TTCTTAACAT CTGTTAGTGA CAAACCTACA 
GTGACATTTC CTGTTAAAGA GACTCTGCCA TCACAGTTTT GTGATAAAAC TTATGTGGGT 
TTTGACATGG GAATATGTAA AACTGAAAAC ACAGGAAAAG AAATAGAGGA GTATAGTGAC 
ACATTAAGCA ATTTTGAATC TTTCAAGTGG ACTGATGCTG TATACGGAGA TAATAAAGAA 
GATGCTAATA AACCTATTCA AGAAGTCAGA ATTAACTATG ATTTTGCTTT ACCCCCGAAT 
AAATCTAAAG GTATAAACAT GAATTTACAT ATTCCTCATA TTCAAAACCA GATTGCTGAA 
AACAGTAATC TATTTTCAGA AGATGCTCAA CCTCAGCCTT TTATTTTGGG AAGTGACTTA 
GACAAAAATG ATGAACATGT TTTACCCTCA ACTTCTAGTA GTGACAACAG TGTCCAACTA 
TTAAATACCA ATCAGAATCA CTATGAATGT ATTGCACTTG ATAAATCAAA TACCCACATT 
TCCAATTCTT TGTTTTACCC ACTGGGAAAA AAGCATCTTA TTTCCAAAGA CACAGAAGCA 
CATATATCTG AACCTGAAGA TTTGGGAAAG ATTAGAAGTC CACCCCCTGA CCATGTTGAG 
GTTGAAACTG CCAGAGAAGG CAAACAATAC TGGAATCCTA GAAATTCTTC AGAACTTTCT 
GGATTAGTAA ATACCATTTC TATTTTAAAG TCGCTATGTG AACACAGTAC TGCTTTAGAC 
AGCTTGGAAA TATTGAAAAA GAAAAATACT GTCTTTCAGC AAGGAACTCA ACAGACGTAT 
GAGCCAGATA GCCCTCCTGA AGTGAGGAAA CCATTTATTA CAGTAGTTTC ACCAAAGTCT 
CCTCATCTGC ACAAGGATTC TCAACAGTTC TCTTCCTCGG GAAGTAAAGA AGAGACTGCT 
TTTCAAGCTG TTATTCCTAA ACAAATAGAG AGAAAAACCT GTGACCCTAA GCCTGTTGAG 
TTTCAAGGAC ATCAAGTAAA AGGATCAGCT ACTAGTGGTG TAATGGTCAG AGGACACAGC 
TCACAGCTAG GATGCAGTCA GTTTCCAGAT AGCACTGAGT ATGAAAACTT TATGACAGAG 
ACACCAGAGT TACCTAGCAC GTGTATGCAG ATTGACTTCT TGCAGGTGAC ATCACCAGAA 
GAAAACATCT CTACATTGAG CCCTGTTTCT ACCTTTTCTT TGAACTCAAG AGATGAAGAC 
TTCATGGTAG AATTCTCTGA GACGTCCCTG AAAGCAAGAA CTTTACCTGA TGATCTTCAT 
TTTCTCAACT TGGAGGGGAT GAAAAAATCC CGTTCTCTGG AGAATGAGAA CCTTCAAAGG 
CTTTCATTAT TAAGTAGAAC CCAGGTTCCA CTTATTACTT TGCCACGTAC TGATGGGCCA 
CCTGACTTAG ACTCTCATTC GTATATGATC AACTCTAACA CATACGAGTC TTCTGGCTCC 
CCCATGCTCA ATTTGTGTGA AAAGTCAGCA GTTCTTTCGT TTAGCATTGA GCCTGAGGAC 
CAAAATGAAA CCTTTTTCTC TGAAGAATCT AGGGAAGTGA ATCCAGGGGA TGTTTCACTT 
AATAATATAT CTACTCAGAG CAAGTGGCTG AAATATCAAA ACACATCCCA ATGCAACGTG 
GCTACTCCAA ACAGAGTTGA TAAGAGAATA ACTGATGGCT TCTTTGCTGA GGCTGTTTCT 
GGGATGCATT TTAGAGACAC AAGTGAAAGA CAGAGTGATG CTGTCAATGA AAGCTCTTTA 
GACTCTGTGC ATTTGCAAAT GATAAAAGGC ATGCTCTATC AACAGCGGCA GGATTTTAGC 
AGTCAAGATT CGGTTTCCAG AAAGAAAGTA CTTTCTCTGA ATTTAAAGCA GACTTCTAAG 
ACAGAGGAAA TTAAAAATGT ATTAGGAGGG TCTACCTGCT ACAACTACAG TGTAAAGGAT 
TTACAGGAGA TAAGTGGCTC TGAGCTGTGC TTTCCAAGTG GGCAGAAAAT AAAATCTGCT 
TATCTTCCCC AAAGGCAAAT TCACATACCA GCTGTTTTTC AGTCTCCTGC TCATTATAAG 
CAGACTTTCA CATCTTGCCT CATAGAACAT CTAAATATAT TGCTGTTTGG GTTAGCACAA 
AACCTGCAGA AAGCTCTTTC AAAAGTTGAC ATATCATTTT ATACATCATT GAAGGGAGAG 
AAACTGAAAA ACGCAGAAAA TAATGTACCA TCCTGCCATC ATAGTCAACC TGCAAAACTT 
GTCATGGTTA AAAAGGAAGG TCCAAATAAG GGTCGTCTCT TTTATACATG TGATGGACCC 
AAAGCTGATC GATGTAAATT CTTTAAATGG CTTGAGGACG TGACTCCAGG ATATTCAACA 
CAGGAAGGAG CTCGACCTGG CATGGTTTTA AGTGATATTA AGAGTATTGG CTTATATTTA 
AGAAGTCAAA AGATACCACT TTATGAGGAA TGCCAGCTTT TGGTGAGAAA AGGATTTGAT 
TTTCAGAGAA AACAGTATGG CAAACTAAAG AAGTTTACTA CTGTAAATCC TGAGTTTTAT 
AATGAACCAA AAACCAAACT TTATCTTAAG CTAAGTCGGA AGGAAAGATC TTCAGCTTAT 
AGCAAAAATG ATCTTTGGGT GGTTTCAAAA ACCCTAGACT TTGAGCTGGA TACTTTTATC 
GCATGTAGTG CTTTCTTTGG ACCATCATCT ATCAATGAGA TAGAAATACT GCCTTTGAAA 
GGCTATTTCC CTTCTAATTG GCCCACTAAC ATGGTTGTCC ATGCGTTATT GGTTTGTAAT 
GCTAGCACAG AACTGACTAC TTTGAAAAAC ATTCAGGACT ACTTTAATCC AGCTACTCTA 
CCTCTAACAC AGTACCTGTT AACAACGTCT TCGCCAACTA TAGTTAGTAA CAAAAGAGTC 
AGTAAGAGAA AATTTATCCC ACCAGCCTTC ACAAATGTCA GTACAAAATT TGAACTACTC 
AGCCTAGGAG CAACATTGAA GTTAGCTAGT GAGTTGATTC AGGTACACAA GTTAAACAAG 
GATCAAGCTA CAGCTCTAAT TCAAATAGCT CAAATGATGG CATCACATGA AAGCATTGAA 
GAAGTGAAGG AACTGCAAAC TCATACCTTC CCTATCACAA TCATACATGG TGTGTTTGGA 
GCAGGAAAGA GTTACTTGCT GGCAGTGGTG ATTTTGTTCT TTGTACAGCT GTTTGAAAAG 
AGTGAAGCTC CCACCATTGG AAATGCAAGG CCGTGGAAAC TTCTGATTTC TTCTTCTACT 
AATGTGGCTG TTGACAGAGT ACTTCTTGGG CTTCTCAGTC TTGGATTTGA AAACTTTATC 
AGAGTTGGGA GTGTTAGGAA GATTGCCAAA CCAATTTTAC CTTATAGCTT GCATGCTGGC 
TCAGAAAATG AAAGTGAACA GTTAAAAGAA CTACATGCAC TAATGAAAGA AGACCTGACT 
CCTACGGAAA GAGTCTATGT GAGAAAAAGC ATTGAGCAGC ATAAACTGGG GACCAATAGA 
ACCCTGCTGA AGCAGGTTCG AGTAGTTGGA GTTACCTGTG CAGCCTGCCC ATTCCCATGC 
ATGAATGATC TTAAATTTCC TGTAGTTGTG CTGGATGAGT GTAGTCAGAT AACTGAACCG 
GCCTCTCTCC TTCCCATTGC AAGGTTTGAG TGTGAAAAGC TGATTCTTGT TGGGGATCCC 
AAACAGCTAC CTCCTACTAT TCAGGGTTCT GATGCAGCTC ATGAAAATGG ATTGGAACAA 
ACTCTTTTTG ATCGACTTTG CTTAATGGGT CACAAGCCAA TTCTATTGAG AACTCAATAC 
CGTTGTCATC CTGCAATCAG TGCTATTGCT AATGATCTGT TTTACAAAGG AGCCCTCATG 
AATGGTGTAA CAGAAATAGA GCGGAGCCCT TTATTGGAAT GGCTACCAAC CCTGTGTTTT 
TATAATGTTA AAGGACTAGA ACAGATAGAA AGAGATAACA GCTTTCATAA TGTGGCAGAA 
GCTACGTTTA CACTCAAGCT GATTCAATCA CTGATTGCAA GTGGAATAGC AGGCTCTATG 
ATTGGTGTGA TAACATTATA CAAATCCCAG ATGTACAAGC TTTGTCATTT ACTCAGTGCT 
GTGGACTTTC ACCATCCTGA TATTAAAACT GTGCAGGTGT CCACAGTAGA TGCTTTTCAG 
GGAGCTGAAA AGGAGATCAT TATTCTGTCC TGTGTAAGGA CAAGACAAGT AGGATTCATT 
GATTCAGAAA AAAGAATGAA TGTTGCATTG ACTAGAGGAA AGAGGCATTT GTTGATTGTG 
GGAAATTTAG CCTGTTTGAG GAAAAATCAA CTTTGGGGAC GAGTGATCCA ACACTGCGAA 
GGAAGGGAAG ATGGATTGCA ACATGCAAAC CAGTATGAAC CACAGCTGAA CCATCTCCTT 
AAAGATTATT TTGAAAAACA AGTGGAAGAA AAACAGAAGA AAAAGAGTGA AAAAGAGAAA 
TCTAAAGATA AATCTCATTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_017008369.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863858.1 Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X4, mRNA.

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CloneID	OHu69372
Clone ID Related Accession (Same CDS sequence)	XM_017008369.1 , XM_017008369.2 , XM_011532092.2
Accession Version	XM_017008369.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein ZGRF1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGCC AAGAATTTAT TGTTCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGTGAAACC TGGAGATGAC 
TTAGAAAGTG ATCGATACTT AATCACAGTT GAAGAGGTTA AAGTTGCTGG AGCCATAGGT 
ATTGTTAAGC AGAATGTCAA TAAAGAAGCA CCAGAGTTAA ATTCAAGGAC ATTTATATCC 
TCTGGCCGAT CTCTTGGATG TCAGCCCTCT GGCTTAAAAA GGAAGTTTAC TGGTTTTCAA 
GGACCACGTC AGGTTCCAAA GAAAATGGTT ATTATGGAAA GTGGTGAATC AGCTGCATCA 
CATGAGGCTA AGAAAACTGG CCCTACTATT TTTTCTCCAT TCTGCAGCAT GCCTCCTTTG 
TTTCCTACTG TTGGCAAGAA AGATGTAAAT AATATACTGG CAGACCCTGA GAACATTGTG 
ACTTACAAGA ACAGGGAGAG AAATGCCATG GATTTTTCTT CGGTTTTTTC TCCATCCTTC 
CAGATTAACC CAGAAGTGCT GTGTGAAGAA AATTATTTTT GCTCACCTGT CAATTCTGGA 
AATAAGCTTT CAGACTCTTT ACTGACCAAT GAGCCTGTGA AAAGAGATAG TTTGGCATCT 
CACTATTCAG GAGTTTCACA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTGAAG 
TCCGAATCAT CTAGTTCATG TGAGGAACTA AATTCTGAGA TGACAGAGCA TTTTCCTCAA 
AAACAACCAC AAGGAAGTTT AAAAATTGCT ACTAAACCAA AGTACCTAAT TCAACAGGAA 
GAGTGTGCTG AGATGAAGAG CACAGAAAAT TTATACTACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TATACATTCT 
TCTACTGTAG ATGGGAATGA TACAGAAAGG AAACCCAAGG CCCAGGAAGA TGATGTAAAT 
TCTAATTTGA AAGACCTTTC ATTACAAAAA ATTATACAGT TCGTTGAAAC GTATGCTGAA 
GAGAGGAAAA AGTATAATGT AGACCAGTCA GTCGGTAATA ATGATCCATC CTGGAATCAG 
GAAGTAAAAT TAGAAATTCC TTCATTCAAT GAAAGCAGTA GCTTACAGGT TACTTGTAGC 
AGTGCAGAAA ATGATGGTAT ATTATCAGAA TCTGACATTC AAGAAGATAA TAAAATACCT 
TTTAATCAAA ATGACAAGGG GTGCATTAAA GGATCAGTTC TCATTAAAGA AAATGCTCAG 
GAGGTAAATA CATGTGGAAC ACTGGAAAAG GAGTATGAAC AATCAGAGTC ATCTCTGCCA 
GAACTGAAAC ATCTCCAAAT TGAATCTAGT AATAATTCTA GGATCTCTGA TGACATTACA 
GACATGATTT CTGAAAGTAA AATGGATAAT GAAAGTCTTA ATAGTATTCA TGAATCTCTG 
AGTAATGTAA CACAACCATT TTTGGAGGTA ACTTTTAATC TGAACAATTT TGAGACCAGT 
GACACTGAGG AGGAATCACA GGAAAGCAAC AAAATTTCCC AGGATTCAGA GAGTTGGGTA 
AAGGACATTT TGGTTAATGA TGGCAATTCA TGTTTTCAAA AGAGGTCTGA GAATACAAAC 
TGTGAAGAGA TTGAGGGTGA ACATTTGCCA TTCTTAACAT CTGTTAGTGA CAAACCTACA 
GTGACATTTC CTGTTAAAGA GACTCTGCCA TCACAGTTTT GTGATAAAAC TTATGTGGGT 
TTTGACATGG GAATATGTAA AACTGAAAAC ACAGGAAAAG AAATAGAGGA GTATAGTGAC 
ACATTAAGCA ATTTTGAATC TTTCAAGTGG ACTGATGCTG TATACGGAGA TAATAAAGAA 
GATGCTAATA AACCTATTCA AGAAGTCAGA ATTAACTATG ATTTTGCTTT ACCCCCGAAT 
AAATCTAAAG GTATAAACAT GAATTTACAT ATTCCTCATA TTCAAAACCA GATTGCTGAA 
AACAGTAATC TATTTTCAGA AGATGCTCAA CCTCAGCCTT TTATTTTGGG AAGTGACTTA 
GACAAAAATG ATGAACATGT TTTACCCTCA ACTTCTAGTA GTGACAACAG TGTCCAACTA 
TTAAATACCA ATCAGAATCA CTATGAATGT ATTGCACTTG ATAAATCAAA TACCCACATT 
TCCAATTCTT TGTTTTACCC ACTGGGAAAA AAGCATCTTA TTTCCAAAGA CACAGAAGCA 
CATATATCTG AACCTGAAGA TTTGGGAAAG ATTAGAAGTC CACCCCCTGA CCATGTTGAG 
GTTGAAACTG CCAGAGAAGG CAAACAATAC TGGAATCCTA GAAATTCTTC AGAACTTTCT 
GGATTAGTAA ATACCATTTC TATTTTAAAG TCGCTATGTG AACACAGTAC TGCTTTAGAC 
AGCTTGGAAA TATTGAAAAA GAAAAATACT GTCTTTCAGC AAGGAACTCA ACAGACGTAT 
GAGCCAGATA GCCCTCCTGA AGTGAGGAAA CCATTTATTA CAGTAGTTTC ACCAAAGTCT 
CCTCATCTGC ACAAGGATTC TCAACAGATA CTAAAAGAAG ATGAAGTTGA ACTAAGTGAA 
CCACTTCAGT CTGTGCAGTT CTCTTCCTCG GGAAGTAAAG AAGAGACTGC TTTTCAAGCT 
GTTATTCCTA AACAAATAGA GAGAAAAACC TGTGACCCTA AGCCTGTTGA GTTTCAAGGA 
CATCAAGTAA AAGGATCAGC TACTAGTGGT GTAATGGTCA GAGGACACAG CTCACAGCTA 
GGATGCAGTC AGTTTCCAGA TAGCACTGAG TATGAAAACT TTATGACAGA GACACCAGAG 
TTACCTAGCA CGTGTATGCA GATTGACTTC TTGCAGGTGA CATCACCAGA AGAAAACATC 
TCTACATTGA GCCCTGTTTC TACCTTTTCT TTGAACTCAA GAGATGAAGA CTTCATGGTA 
GAATTCTCTG AGACGTCCCT GAAAGCAAGA ACTTTACCTG ATGATCTTCA TTTTCTCAAC 
TTGGAGGGGA TGAAAAAATC CCGTTCTCTG GAGAATGAGA ACCTTCAAAG GCTTTCATTA 
TTAAGTAGAA CCCAGGTTCC ACTTATTACT TTGCCACGTA CTGATGGGCC ACCTGACTTA 
GACTCTCATT CGTATATGAT CAACTCTAAC ACATACGAGT CTTCTGGCTC CCCCATGCTC 
AATTTGTGTG AAAAGTCAGC AGTTCTTTCG TTTAGCATTG AGCCTGAGGA CCAAAATGAA 
ACCTTTTTCT CTGAAGAATC TAGGGAAGTG AATCCAGGGG ATGTTTCACT TAATAATATA 
TCTACTCAGA GCAAGTGGCT GAAATATCAA AACACATCCC AATGCAACGT GGCTACTCCA 
AACAGAGTTG ATAAGAGAAT AACTGATGGC TTCTTTGCTG AGGCTGTTTC TGGGATGCAT 
TTTAGAGACA CAAGTGAAAG ACAGAGTGAT GCTGTCAATG AAAGCTCTTT AGACTCTGTG 
CATTTGCAAA TGATAAAAGG CATGCTCTAT CAACAGCGGC AGGATTTTAG CAGTCAAGAT 
TCGGTTTCCA GAAAGAAAGT ACTTTCTCTG AATTTAAAGC AGACTTCTAA GACAGAGGAA 
ATTAAAAATG TATTAGGAGG GTCTACCTGC TACAACTACA GTGTAAAGGA TTTACAGGAG 
ATAAGTGGCT CTGAGCTGTG CTTTCCAAGT GGGCAGAAAA TAAAATCTGC TTATCTTCCC 
CAAAGGCAAA TTCACATACC AGCTGTTTTT CAGTCTCCTG CTCATTATAA GCAGACTTTC 
ACATCTTGCC TCATAGAACA TCTAAATATA TTGCTGTTTG GGTTAGCACA AAACCTGCAG 
AAAGCTCTTT CAAAAGTTGA CATATCATTT TATACATCAT TGAAGGGAGA GAAACTGAAA 
AACGCAGAAA ATAATGTACC ATCCTGCCAT CATAGTCAAC CTGCAAAACT TGTCATGGTT 
AAAAAGGAAG GTCCAAATAA GGGTCGTCTC TTTTATACAT GTGATGGACC CAAAGCTGAT 
CGATGTAAAT TCTTTAAATG GCTTGAGGAC GTGACTCCAG GATATTCAAC ACAGGAAGGA 
GCTCGACCTG GCATGGTTTT AAGTGATATT AAGAGTATTG GCTTATATTT AAGAAGTCAA 
AAGATACCAC TTTATGAGGA ATGCCAGCTT TTGGTGAGAA AAGGATTTGA TTTTCAGAGA 
AAACAGTATG GCAAACTAAA GAAGTTTACT ACTGTAAATC CTGAGTTTTA TAATGAACCA 
AAAACCAAAC TTTATCTTAA GCTAAGTCGG AAGGAAAGAT CTTCAGCTTA TAGCAAAAAT 
GATCTTTGGG TGGTTTCAAA AACCCTAGAC TTTGAGCTGG ATACTTTTAT CGCATGTAGT 
GCTTTCTTTG GACCATCATC TATCAATGAG ATAGAAATAC TGCCTTTGAA AGGCTATTTC 
CCTTCTAATT GGCCCACTAA CATGGTTGTC CATGCGTTAT TGGTTTGTAA TGCTAGCACA 
GAACTGACTA CTTTGAAAAA CATTCAGGAC TACTTTAATC CAGCTACTCT ACCTCTAACA 
CAGTACCTGT TAACAACGTC TTCGCCAACT ATAGTTAGTA ACAAAAGAGT CAGTAAGAGA 
AAATTTATCC CACCAGCCTT CACAAATGTC AGTACAAAAT TTGAACTACT CAGCCTAGGA 
GCAACATTGA AGTTAGCTAG TGAGTTGATT CAGGTACACA AGTTAAACAA GGATCAAGCT 
ACAGCTCTAA TTCAAATAGC TCAAATGATG GCATCACATG AAAGCATTGA AGAAGTGAAG 
GAACTGCAAA CTCATACCTT CCCTATCACA ATCATACATG GTGTGTTTGG AGCAGGAAAG 
AGTTACTTGC TGGCAGTGGT GATTTTGTTC TTTGTACAGC TGTTTGAAAA GAGTGAAGCT 
CCCACCATTG GAAATGCAAG GCCGTGGAAA CTTCTGATTT CTTCTTCTAC TAATGTGGCT 
GTTGACAGAG TACTTCTTGG GCTTCTCAGT CTTGGATTTG AAAACTTTAT CAGAGTTGGG 
AGTGTTAGGA AGATTGCCAA ACCAATTTTA CCTTATAGCT TGCATGCTGG CTCAGAAAAT 
GAAAGTGAAC AGTTAAAAGA ACTACATGCA CTAATGAAAG AAGACCTGAC TCCTACGGAA 
AGAGTCTATG TGAGAAAAAG CATTGAGCAG CATAAACTGG GGACCAATAG AACCCTGCTG 
AAGCAGGTTC GAGTAGTTGG AGTTACCTGT GCAGCCTGCC CATTCCCATG CATGAATGAT 
CTTAAATTTC CTGTAGTTGT GCTGGATGAG TGTAGTCAGA TAACTGAACC GGCCTCTCTC 
CTTCCCATTG CAAGGTTTGA GTGTGAAAAG CTGATTCTTG TTGGGGATCC CAAACAGCTA 
CCTCCTACTA TTCAGGGTTC TGATGCAGCT CATGAAAATG GATTGGAACA AACTCTTTTT 
GATCGACTTT GCTTAATGGG TCACAAGCCA ATTCTATTGA GAACTCAATA CCGTTGTCAT 
CCTGCAATCA GTGCTATTGC TAATGATCTG TTTTACAAAG GAGCCCTCAT GAATGGTGTA 
ACAGAAATAG AGCGGAGCCC TTTATTGGAA TGGCTACCAA CCCTGTGTTT TTATAATGTT 
AAAGGACTAG AACAGATAGA AAGAGATAAC AGCTTTCATA ATGTGGCAGA AGCTACGTTT 
ACACTCAAGC TGATTCAATC ACTGATTGCA AGTGGAATAG CAGGCTCTAT GATTGGTGTG 
ATAACATTAT ACAAATCCCA GATGTACAAG CTTTGTCATT TACTCAGTGC TGTGGACTTT 
CACCATCCTG ATATTAAAAC TGTGCAGGTG TCCACAGTAG ATGCTTTTCA GGGAGCTGAA 
AAGGAGATCA TTATTCTGTC CTGTGTAAGG ACAAGACAAG TAGGATTCAT TGATTCAGAA 
AAAAGAATGA ATGTTGCATT GACTAGAGGA AAGAGGCATT TGTTGATTGT GGGAAATTTA 
GCCTGTTTGA GGAAAAATCA ACTTTGGGGA CGAGTGATCC AACACTGCGA AGGAAGGGAA 
GATGGATTGC AACATGCAAA CCAGTATGAA CCACAGCTGA ACCATCTCCT TAAAGATTAT 
TTTGAAAAAC AAGTGGAAGA AAAACAGAAG AAAAAGAGTG AAAAAGAGAA ATCTAAAGAT 
AAATCTCATT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863858.1
CDS	267..6521
Translation	MESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKVKPGDDLESDRYLITVEEVKVAGAIGIVKQNVNKEAPELNSRTFISSGRSLGCQPSGLKRKFTGFQGPRQVPKKMVIMESGESAASHEAKKTGPTIFSPFCSMPPLFPTVGKKDVNNILADPENIVTYKNRERNAMDFSSVFSPSFQINPEVLCEENYFCSPVNSGNKLSDSLLTNEPVKRDSLASHYSGVSQNIRSKAQILALLKSESSSSCEELNSEMTEHFPQKQPQGSLKIATKPKYLIQQEECAEMKSTENLYYQHQSENTMRNKSRWAMYLSSQSSPIHSSTVDGNDTERKPKAQEDDVNSNLKDLSLQKIIQFVETYAEERKKYNVDQSVGNNDPSWNQEVKLEIPSFNESSSLQVTCSSAENDGILSESDIQEDNKIPFNQNDKGCIKGSVLIKENAQEVNTCGTLEKEYEQSESSLPELKHLQIESSNNSRISDDITDMISESKMDNESLNSIHESLSNVTQPFLEVTFNLNNFETSDTEEESQESNKISQDSESWVKDILVNDGNSCFQKRSENTNCEEIEGEHLPFLTSVSDKPTVTFPVKETLPSQFCDKTYVGFDMGICKTENTGKEIEEYSDTLSNFESFKWTDAVYGDNKEDANKPIQEVRINYDFALPPNKSKGINMNLHIPHIQNQIAENSNLFSEDAQPQPFILGSDLDKNDEHVLPSTSSSDNSVQLLNTNQNHYECIALDKSNTHISNSLFYPLGKKHLISKDTEAHISEPEDLGKIRSPPPDHVEVETAREGKQYWNPRNSSELSGLVNTISILKSLCEHSTALDSLEILKKKNTVFQQGTQQTYEPDSPPEVRKPFITVVSPKSPHLHKDSQQILKEDEVELSEPLQSVQFSSSGSKEETAFQAVIPKQIERKTCDPKPVEFQGHQVKGSATSGVMVRGHSSQLGCSQFPDSTEYENFMTETPELPSTCMQIDFLQVTSPEENISTLSPVSTFSLNSRDEDFMVEFSETSLKARTLPDDLHFLNLEGMKKSRSLENENLQRLSLLSRTQVPLITLPRTDGPPDLDSHSYMINSNTYESSGSPMLNLCEKSAVLSFSIEPEDQNETFFSEESREVNPGDVSLNNISTQSKWLKYQNTSQCNVATPNRVDKRITDGFFAEAVSGMHFRDTSERQSDAVNESSLDSVHLQMIKGMLYQQRQDFSSQDSVSRKKVLSLNLKQTSKTEEIKNVLGGSTCYNYSVKDLQEISGSELCFPSGQKIKSAYLPQRQIHIPAVFQSPAHYKQTFTSCLIEHLNILLFGLAQNLQKALSKVDISFYTSLKGEKLKNAENNVPSCHHSQPAKLVMVKKEGPNKGRLFYTCDGPKADRCKFFKWLEDVTPGYSTQEGARPGMVLSDIKSIGLYLRSQKIPLYEECQLLVRKGFDFQRKQYGKLKKFTTVNPEFYNEPKTKLYLKLSRKERSSAYSKNDLWVVSKTLDFELDTFIACSAFFGPSSINEIEILPLKGYFPSNWPTNMVVHALLVCNASTELTTLKNIQDYFNPATLPLTQYLLTTSSPTIVSNKRVSKRKFIPPAFTNVSTKFELLSLGATLKLASELIQVHKLNKDQATALIQIAQMMASHESIEEVKELQTHTFPITIIHGVFGAGKSYLLAVVILFFVQLFEKSEAPTIGNARPWKLLISSSTNVAVDRVLLGLLSLGFENFIRVGSVRKIAKPILPYSLHAGSENESEQLKELHALMKEDLTPTERVYVRKSIEQHKLGTNRTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPAISAIANDLFYKGALMNGVTEIERSPLLEWLPTLCFYNVKGLEQIERDNSFHNVAEATFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAVDFHHPDIKTVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGKRHLLIVGNLACLRKNQLWGRVIQHCEGREDGLQHANQYEPQLNHLLKDYFEKQVEEKQKKKSEKEKSKDKSHS

Target ORF information:

RefSeq Version	XM_017008369.1
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008369.1

ORF Insert Sequence:

ATGGAAAGCC AAGAATTTAT TGTTCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGTGAAACC TGGAGATGAC 
TTAGAAAGTG ATCGATACTT AATCACAGTT GAAGAGGTTA AAGTTGCTGG AGCCATAGGT 
ATTGTTAAGC AGAATGTCAA TAAAGAAGCA CCAGAGTTAA ATTCAAGGAC ATTTATATCC 
TCTGGCCGAT CTCTTGGATG TCAGCCCTCT GGCTTAAAAA GGAAGTTTAC TGGTTTTCAA 
GGACCACGTC AGGTTCCAAA GAAAATGGTT ATTATGGAAA GTGGTGAATC AGCTGCATCA 
CATGAGGCTA AGAAAACTGG CCCTACTATT TTTTCTCCAT TCTGCAGCAT GCCTCCTTTG 
TTTCCTACTG TTGGCAAGAA AGATGTAAAT AATATACTGG CAGACCCTGA GAACATTGTG 
ACTTACAAGA ACAGGGAGAG AAATGCCATG GATTTTTCTT CGGTTTTTTC TCCATCCTTC 
CAGATTAACC CAGAAGTGCT GTGTGAAGAA AATTATTTTT GCTCACCTGT CAATTCTGGA 
AATAAGCTTT CAGACTCTTT ACTGACCAAT GAGCCTGTGA AAAGAGATAG TTTGGCATCT 
CACTATTCAG GAGTTTCACA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTGAAG 
TCCGAATCAT CTAGTTCATG TGAGGAACTA AATTCTGAGA TGACAGAGCA TTTTCCTCAA 
AAACAACCAC AAGGAAGTTT AAAAATTGCT ACTAAACCAA AGTACCTAAT TCAACAGGAA 
GAGTGTGCTG AGATGAAGAG CACAGAAAAT TTATACTACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TATACATTCT 
TCTACTGTAG ATGGGAATGA TACAGAAAGG AAACCCAAGG CCCAGGAAGA TGATGTAAAT 
TCTAATTTGA AAGACCTTTC ATTACAAAAA ATTATACAGT TCGTTGAAAC GTATGCTGAA 
GAGAGGAAAA AGTATAATGT AGACCAGTCA GTCGGTAATA ATGATCCATC CTGGAATCAG 
GAAGTAAAAT TAGAAATTCC TTCATTCAAT GAAAGCAGTA GCTTACAGGT TACTTGTAGC 
AGTGCAGAAA ATGATGGTAT ATTATCAGAA TCTGACATTC AAGAAGATAA TAAAATACCT 
TTTAATCAAA ATGACAAGGG GTGCATTAAA GGATCAGTTC TCATTAAAGA AAATGCTCAG 
GAGGTAAATA CATGTGGAAC ACTGGAAAAG GAGTATGAAC AATCAGAGTC ATCTCTGCCA 
GAACTGAAAC ATCTCCAAAT TGAATCTAGT AATAATTCTA GGATCTCTGA TGACATTACA 
GACATGATTT CTGAAAGTAA AATGGATAAT GAAAGTCTTA ATAGTATTCA TGAATCTCTG 
AGTAATGTAA CACAACCATT TTTGGAGGTA ACTTTTAATC TGAACAATTT TGAGACCAGT 
GACACTGAGG AGGAATCACA GGAAAGCAAC AAAATTTCCC AGGATTCAGA GAGTTGGGTA 
AAGGACATTT TGGTTAATGA TGGCAATTCA TGTTTTCAAA AGAGGTCTGA GAATACAAAC 
TGTGAAGAGA TTGAGGGTGA ACATTTGCCA TTCTTAACAT CTGTTAGTGA CAAACCTACA 
GTGACATTTC CTGTTAAAGA GACTCTGCCA TCACAGTTTT GTGATAAAAC TTATGTGGGT 
TTTGACATGG GAATATGTAA AACTGAAAAC ACAGGAAAAG AAATAGAGGA GTATAGTGAC 
ACATTAAGCA ATTTTGAATC TTTCAAGTGG ACTGATGCTG TATACGGAGA TAATAAAGAA 
GATGCTAATA AACCTATTCA AGAAGTCAGA ATTAACTATG ATTTTGCTTT ACCCCCGAAT 
AAATCTAAAG GTATAAACAT GAATTTACAT ATTCCTCATA TTCAAAACCA GATTGCTGAA 
AACAGTAATC TATTTTCAGA AGATGCTCAA CCTCAGCCTT TTATTTTGGG AAGTGACTTA 
GACAAAAATG ATGAACATGT TTTACCCTCA ACTTCTAGTA GTGACAACAG TGTCCAACTA 
TTAAATACCA ATCAGAATCA CTATGAATGT ATTGCACTTG ATAAATCAAA TACCCACATT 
TCCAATTCTT TGTTTTACCC ACTGGGAAAA AAGCATCTTA TTTCCAAAGA CACAGAAGCA 
CATATATCTG AACCTGAAGA TTTGGGAAAG ATTAGAAGTC CACCCCCTGA CCATGTTGAG 
GTTGAAACTG CCAGAGAAGG CAAACAATAC TGGAATCCTA GAAATTCTTC AGAACTTTCT 
GGATTAGTAA ATACCATTTC TATTTTAAAG TCGCTATGTG AACACAGTAC TGCTTTAGAC 
AGCTTGGAAA TATTGAAAAA GAAAAATACT GTCTTTCAGC AAGGAACTCA ACAGACGTAT 
GAGCCAGATA GCCCTCCTGA AGTGAGGAAA CCATTTATTA CAGTAGTTTC ACCAAAGTCT 
CCTCATCTGC ACAAGGATTC TCAACAGATA CTAAAAGAAG ATGAAGTTGA ACTAAGTGAA 
CCACTTCAGT CTGTGCAGTT CTCTTCCTCG GGAAGTAAAG AAGAGACTGC TTTTCAAGCT 
GTTATTCCTA AACAAATAGA GAGAAAAACC TGTGACCCTA AGCCTGTTGA GTTTCAAGGA 
CATCAAGTAA AAGGATCAGC TACTAGTGGT GTAATGGTCA GAGGACACAG CTCACAGCTA 
GGATGCAGTC AGTTTCCAGA TAGCACTGAG TATGAAAACT TTATGACAGA GACACCAGAG 
TTACCTAGCA CGTGTATGCA GATTGACTTC TTGCAGGTGA CATCACCAGA AGAAAACATC 
TCTACATTGA GCCCTGTTTC TACCTTTTCT TTGAACTCAA GAGATGAAGA CTTCATGGTA 
GAATTCTCTG AGACGTCCCT GAAAGCAAGA ACTTTACCTG ATGATCTTCA TTTTCTCAAC 
TTGGAGGGGA TGAAAAAATC CCGTTCTCTG GAGAATGAGA ACCTTCAAAG GCTTTCATTA 
TTAAGTAGAA CCCAGGTTCC ACTTATTACT TTGCCACGTA CTGATGGGCC ACCTGACTTA 
GACTCTCATT CGTATATGAT CAACTCTAAC ACATACGAGT CTTCTGGCTC CCCCATGCTC 
AATTTGTGTG AAAAGTCAGC AGTTCTTTCG TTTAGCATTG AGCCTGAGGA CCAAAATGAA 
ACCTTTTTCT CTGAAGAATC TAGGGAAGTG AATCCAGGGG ATGTTTCACT TAATAATATA 
TCTACTCAGA GCAAGTGGCT GAAATATCAA AACACATCCC AATGCAACGT GGCTACTCCA 
AACAGAGTTG ATAAGAGAAT AACTGATGGC TTCTTTGCTG AGGCTGTTTC TGGGATGCAT 
TTTAGAGACA CAAGTGAAAG ACAGAGTGAT GCTGTCAATG AAAGCTCTTT AGACTCTGTG 
CATTTGCAAA TGATAAAAGG CATGCTCTAT CAACAGCGGC AGGATTTTAG CAGTCAAGAT 
TCGGTTTCCA GAAAGAAAGT ACTTTCTCTG AATTTAAAGC AGACTTCTAA GACAGAGGAA 
ATTAAAAATG TATTAGGAGG GTCTACCTGC TACAACTACA GTGTAAAGGA TTTACAGGAG 
ATAAGTGGCT CTGAGCTGTG CTTTCCAAGT GGGCAGAAAA TAAAATCTGC TTATCTTCCC 
CAAAGGCAAA TTCACATACC AGCTGTTTTT CAGTCTCCTG CTCATTATAA GCAGACTTTC 
ACATCTTGCC TCATAGAACA TCTAAATATA TTGCTGTTTG GGTTAGCACA AAACCTGCAG 
AAAGCTCTTT CAAAAGTTGA CATATCATTT TATACATCAT TGAAGGGAGA GAAACTGAAA 
AACGCAGAAA ATAATGTACC ATCCTGCCAT CATAGTCAAC CTGCAAAACT TGTCATGGTT 
AAAAAGGAAG GTCCAAATAA GGGTCGTCTC TTTTATACAT GTGATGGACC CAAAGCTGAT 
CGATGTAAAT TCTTTAAATG GCTTGAGGAC GTGACTCCAG GATATTCAAC ACAGGAAGGA 
GCTCGACCTG GCATGGTTTT AAGTGATATT AAGAGTATTG GCTTATATTT AAGAAGTCAA 
AAGATACCAC TTTATGAGGA ATGCCAGCTT TTGGTGAGAA AAGGATTTGA TTTTCAGAGA 
AAACAGTATG GCAAACTAAA GAAGTTTACT ACTGTAAATC CTGAGTTTTA TAATGAACCA 
AAAACCAAAC TTTATCTTAA GCTAAGTCGG AAGGAAAGAT CTTCAGCTTA TAGCAAAAAT 
GATCTTTGGG TGGTTTCAAA AACCCTAGAC TTTGAGCTGG ATACTTTTAT CGCATGTAGT 
GCTTTCTTTG GACCATCATC TATCAATGAG ATAGAAATAC TGCCTTTGAA AGGCTATTTC 
CCTTCTAATT GGCCCACTAA CATGGTTGTC CATGCGTTAT TGGTTTGTAA TGCTAGCACA 
GAACTGACTA CTTTGAAAAA CATTCAGGAC TACTTTAATC CAGCTACTCT ACCTCTAACA 
CAGTACCTGT TAACAACGTC TTCGCCAACT ATAGTTAGTA ACAAAAGAGT CAGTAAGAGA 
AAATTTATCC CACCAGCCTT CACAAATGTC AGTACAAAAT TTGAACTACT CAGCCTAGGA 
GCAACATTGA AGTTAGCTAG TGAGTTGATT CAGGTACACA AGTTAAACAA GGATCAAGCT 
ACAGCTCTAA TTCAAATAGC TCAAATGATG GCATCACATG AAAGCATTGA AGAAGTGAAG 
GAACTGCAAA CTCATACCTT CCCTATCACA ATCATACATG GTGTGTTTGG AGCAGGAAAG 
AGTTACTTGC TGGCAGTGGT GATTTTGTTC TTTGTACAGC TGTTTGAAAA GAGTGAAGCT 
CCCACCATTG GAAATGCAAG GCCGTGGAAA CTTCTGATTT CTTCTTCTAC TAATGTGGCT 
GTTGACAGAG TACTTCTTGG GCTTCTCAGT CTTGGATTTG AAAACTTTAT CAGAGTTGGG 
AGTGTTAGGA AGATTGCCAA ACCAATTTTA CCTTATAGCT TGCATGCTGG CTCAGAAAAT 
GAAAGTGAAC AGTTAAAAGA ACTACATGCA CTAATGAAAG AAGACCTGAC TCCTACGGAA 
AGAGTCTATG TGAGAAAAAG CATTGAGCAG CATAAACTGG GGACCAATAG AACCCTGCTG 
AAGCAGGTTC GAGTAGTTGG AGTTACCTGT GCAGCCTGCC CATTCCCATG CATGAATGAT 
CTTAAATTTC CTGTAGTTGT GCTGGATGAG TGTAGTCAGA TAACTGAACC GGCCTCTCTC 
CTTCCCATTG CAAGGTTTGA GTGTGAAAAG CTGATTCTTG TTGGGGATCC CAAACAGCTA 
CCTCCTACTA TTCAGGGTTC TGATGCAGCT CATGAAAATG GATTGGAACA AACTCTTTTT 
GATCGACTTT GCTTAATGGG TCACAAGCCA ATTCTATTGA GAACTCAATA CCGTTGTCAT 
CCTGCAATCA GTGCTATTGC TAATGATCTG TTTTACAAAG GAGCCCTCAT GAATGGTGTA 
ACAGAAATAG AGCGGAGCCC TTTATTGGAA TGGCTACCAA CCCTGTGTTT TTATAATGTT 
AAAGGACTAG AACAGATAGA AAGAGATAAC AGCTTTCATA ATGTGGCAGA AGCTACGTTT 
ACACTCAAGC TGATTCAATC ACTGATTGCA AGTGGAATAG CAGGCTCTAT GATTGGTGTG 
ATAACATTAT ACAAATCCCA GATGTACAAG CTTTGTCATT TACTCAGTGC TGTGGACTTT 
CACCATCCTG ATATTAAAAC TGTGCAGGTG TCCACAGTAG ATGCTTTTCA GGGAGCTGAA 
AAGGAGATCA TTATTCTGTC CTGTGTAAGG ACAAGACAAG TAGGATTCAT TGATTCAGAA 
AAAAGAATGA ATGTTGCATT GACTAGAGGA AAGAGGCATT TGTTGATTGT GGGAAATTTA 
GCCTGTTTGA GGAAAAATCA ACTTTGGGGA CGAGTGATCC AACACTGCGA AGGAAGGGAA 
GATGGATTGC AACATGCAAA CCAGTATGAA CCACAGCTGA ACCATCTCCT TAAAGATTAT 
TTTGAAAAAC AAGTGGAAGA AAAACAGAAG AAAAAGAGTG AAAAAGAGAA ATCTAAAGAT 
AAATCTCATT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_017008369.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863858.1 Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X4, mRNA.

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CloneID	OHu69372
Clone ID Related Accession (Same CDS sequence)	XM_017008369.1 , XM_017008369.2 , XM_011532092.2
Accession Version	XM_017008369.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein ZGRF1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017008369.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGCC AAGAATTTAT TGTTCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGTGAAACC TGGAGATGAC 
TTAGAAAGTG ATCGATACTT AATCACAGTT GAAGAGGTTA AAGTTGCTGG AGCCATAGGT 
ATTGTTAAGC AGAATGTCAA TAAAGAAGCA CCAGAGTTAA ATTCAAGGAC ATTTATATCC 
TCTGGCCGAT CTCTTGGATG TCAGCCCTCT GGCTTAAAAA GGAAGTTTAC TGGTTTTCAA 
GGACCACGTC AGGTTCCAAA GAAAATGGTT ATTATGGAAA GTGGTGAATC AGCTGCATCA 
CATGAGGCTA AGAAAACTGG CCCTACTATT TTTTCTCCAT TCTGCAGCAT GCCTCCTTTG 
TTTCCTACTG TTGGCAAGAA AGATGTAAAT AATATACTGG CAGACCCTGA GAACATTGTG 
ACTTACAAGA ACAGGGAGAG AAATGCCATG GATTTTTCTT CGGTTTTTTC TCCATCCTTC 
CAGATTAACC CAGAAGTGCT GTGTGAAGAA AATTATTTTT GCTCACCTGT CAATTCTGGA 
AATAAGCTTT CAGACTCTTT ACTGACCAAT GAGCCTGTGA AAAGAGATAG TTTGGCATCT 
CACTATTCAG GAGTTTCACA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTGAAG 
TCCGAATCAT CTAGTTCATG TGAGGAACTA AATTCTGAGA TGACAGAGCA TTTTCCTCAA 
AAACAACCAC AAGGAAGTTT AAAAATTGCT ACTAAACCAA AGTACCTAAT TCAACAGGAA 
GAGTGTGCTG AGATGAAGAG CACAGAAAAT TTATACTACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TATACATTCT 
TCTACTGTAG ATGGGAATGA TACAGAAAGG AAACCCAAGG CCCAGGAAGA TGATGTAAAT 
TCTAATTTGA AAGACCTTTC ATTACAAAAA ATTATACAGT TCGTTGAAAC GTATGCTGAA 
GAGAGGAAAA AGTATAATGT AGACCAGTCA GTCGGTAATA ATGATCCATC CTGGAATCAG 
GAAGTAAAAT TAGAAATTCC TTCATTCAAT GAAAGCAGTA GCTTACAGGT TACTTGTAGC 
AGTGCAGAAA ATGATGGTAT ATTATCAGAA TCTGACATTC AAGAAGATAA TAAAATACCT 
TTTAATCAAA ATGACAAGGG GTGCATTAAA GGATCAGTTC TCATTAAAGA AAATGCTCAG 
GAGGTAAATA CATGTGGAAC ACTGGAAAAG GAGTATGAAC AATCAGAGTC ATCTCTGCCA 
GAACTGAAAC ATCTCCAAAT TGAATCTAGT AATAATTCTA GGATCTCTGA TGACATTACA 
GACATGATTT CTGAAAGTAA AATGGATAAT GAAAGTCTTA ATAGTATTCA TGAATCTCTG 
AGTAATGTAA CACAACCATT TTTGGAGGTA ACTTTTAATC TGAACAATTT TGAGACCAGT 
GACACTGAGG AGGAATCACA GGAAAGCAAC AAAATTTCCC AGGATTCAGA GAGTTGGGTA 
AAGGACATTT TGGTTAATGA TGGCAATTCA TGTTTTCAAA AGAGGTCTGA GAATACAAAC 
TGTGAAGAGA TTGAGGGTGA ACATTTGCCA TTCTTAACAT CTGTTAGTGA CAAACCTACA 
GTGACATTTC CTGTTAAAGA GACTCTGCCA TCACAGTTTT GTGATAAAAC TTATGTGGGT 
TTTGACATGG GAATATGTAA AACTGAAAAC ACAGGAAAAG AAATAGAGGA GTATAGTGAC 
ACATTAAGCA ATTTTGAATC TTTCAAGTGG ACTGATGCTG TATACGGAGA TAATAAAGAA 
GATGCTAATA AACCTATTCA AGAAGTCAGA ATTAACTATG ATTTTGCTTT ACCCCCGAAT 
AAATCTAAAG GTATAAACAT GAATTTACAT ATTCCTCATA TTCAAAACCA GATTGCTGAA 
AACAGTAATC TATTTTCAGA AGATGCTCAA CCTCAGCCTT TTATTTTGGG AAGTGACTTA 
GACAAAAATG ATGAACATGT TTTACCCTCA ACTTCTAGTA GTGACAACAG TGTCCAACTA 
TTAAATACCA ATCAGAATCA CTATGAATGT ATTGCACTTG ATAAATCAAA TACCCACATT 
TCCAATTCTT TGTTTTACCC ACTGGGAAAA AAGCATCTTA TTTCCAAAGA CACAGAAGCA 
CATATATCTG AACCTGAAGA TTTGGGAAAG ATTAGAAGTC CACCCCCTGA CCATGTTGAG 
GTTGAAACTG CCAGAGAAGG CAAACAATAC TGGAATCCTA GAAATTCTTC AGAACTTTCT 
GGATTAGTAA ATACCATTTC TATTTTAAAG TCGCTATGTG AACACAGTAC TGCTTTAGAC 
AGCTTGGAAA TATTGAAAAA GAAAAATACT GTCTTTCAGC AAGGAACTCA ACAGACGTAT 
GAGCCAGATA GCCCTCCTGA AGTGAGGAAA CCATTTATTA CAGTAGTTTC ACCAAAGTCT 
CCTCATCTGC ACAAGGATTC TCAACAGATA CTAAAAGAAG ATGAAGTTGA ACTAAGTGAA 
CCACTTCAGT CTGTGCAGTT CTCTTCCTCG GGAAGTAAAG AAGAGACTGC TTTTCAAGCT 
GTTATTCCTA AACAAATAGA GAGAAAAACC TGTGACCCTA AGCCTGTTGA GTTTCAAGGA 
CATCAAGTAA AAGGATCAGC TACTAGTGGT GTAATGGTCA GAGGACACAG CTCACAGCTA 
GGATGCAGTC AGTTTCCAGA TAGCACTGAG TATGAAAACT TTATGACAGA GACACCAGAG 
TTACCTAGCA CGTGTATGCA GATTGACTTC TTGCAGGTGA CATCACCAGA AGAAAACATC 
TCTACATTGA GCCCTGTTTC TACCTTTTCT TTGAACTCAA GAGATGAAGA CTTCATGGTA 
GAATTCTCTG AGACGTCCCT GAAAGCAAGA ACTTTACCTG ATGATCTTCA TTTTCTCAAC 
TTGGAGGGGA TGAAAAAATC CCGTTCTCTG GAGAATGAGA ACCTTCAAAG GCTTTCATTA 
TTAAGTAGAA CCCAGGTTCC ACTTATTACT TTGCCACGTA CTGATGGGCC ACCTGACTTA 
GACTCTCATT CGTATATGAT CAACTCTAAC ACATACGAGT CTTCTGGCTC CCCCATGCTC 
AATTTGTGTG AAAAGTCAGC AGTTCTTTCG TTTAGCATTG AGCCTGAGGA CCAAAATGAA 
ACCTTTTTCT CTGAAGAATC TAGGGAAGTG AATCCAGGGG ATGTTTCACT TAATAATATA 
TCTACTCAGA GCAAGTGGCT GAAATATCAA AACACATCCC AATGCAACGT GGCTACTCCA 
AACAGAGTTG ATAAGAGAAT AACTGATGGC TTCTTTGCTG AGGCTGTTTC TGGGATGCAT 
TTTAGAGACA CAAGTGAAAG ACAGAGTGAT GCTGTCAATG AAAGCTCTTT AGACTCTGTG 
CATTTGCAAA TGATAAAAGG CATGCTCTAT CAACAGCGGC AGGATTTTAG CAGTCAAGAT 
TCGGTTTCCA GAAAGAAAGT ACTTTCTCTG AATTTAAAGC AGACTTCTAA GACAGAGGAA 
ATTAAAAATG TATTAGGAGG GTCTACCTGC TACAACTACA GTGTAAAGGA TTTACAGGAG 
ATAAGTGGCT CTGAGCTGTG CTTTCCAAGT GGGCAGAAAA TAAAATCTGC TTATCTTCCC 
CAAAGGCAAA TTCACATACC AGCTGTTTTT CAGTCTCCTG CTCATTATAA GCAGACTTTC 
ACATCTTGCC TCATAGAACA TCTAAATATA TTGCTGTTTG GGTTAGCACA AAACCTGCAG 
AAAGCTCTTT CAAAAGTTGA CATATCATTT TATACATCAT TGAAGGGAGA GAAACTGAAA 
AACGCAGAAA ATAATGTACC ATCCTGCCAT CATAGTCAAC CTGCAAAACT TGTCATGGTT 
AAAAAGGAAG GTCCAAATAA GGGTCGTCTC TTTTATACAT GTGATGGACC CAAAGCTGAT 
CGATGTAAAT TCTTTAAATG GCTTGAGGAC GTGACTCCAG GATATTCAAC ACAGGAAGGA 
GCTCGACCTG GCATGGTTTT AAGTGATATT AAGAGTATTG GCTTATATTT AAGAAGTCAA 
AAGATACCAC TTTATGAGGA ATGCCAGCTT TTGGTGAGAA AAGGATTTGA TTTTCAGAGA 
AAACAGTATG GCAAACTAAA GAAGTTTACT ACTGTAAATC CTGAGTTTTA TAATGAACCA 
AAAACCAAAC TTTATCTTAA GCTAAGTCGG AAGGAAAGAT CTTCAGCTTA TAGCAAAAAT 
GATCTTTGGG TGGTTTCAAA AACCCTAGAC TTTGAGCTGG ATACTTTTAT CGCATGTAGT 
GCTTTCTTTG GACCATCATC TATCAATGAG ATAGAAATAC TGCCTTTGAA AGGCTATTTC 
CCTTCTAATT GGCCCACTAA CATGGTTGTC CATGCGTTAT TGGTTTGTAA TGCTAGCACA 
GAACTGACTA CTTTGAAAAA CATTCAGGAC TACTTTAATC CAGCTACTCT ACCTCTAACA 
CAGTACCTGT TAACAACGTC TTCGCCAACT ATAGTTAGTA ACAAAAGAGT CAGTAAGAGA 
AAATTTATCC CACCAGCCTT CACAAATGTC AGTACAAAAT TTGAACTACT CAGCCTAGGA 
GCAACATTGA AGTTAGCTAG TGAGTTGATT CAGGTACACA AGTTAAACAA GGATCAAGCT 
ACAGCTCTAA TTCAAATAGC TCAAATGATG GCATCACATG AAAGCATTGA AGAAGTGAAG 
GAACTGCAAA CTCATACCTT CCCTATCACA ATCATACATG GTGTGTTTGG AGCAGGAAAG 
AGTTACTTGC TGGCAGTGGT GATTTTGTTC TTTGTACAGC TGTTTGAAAA GAGTGAAGCT 
CCCACCATTG GAAATGCAAG GCCGTGGAAA CTTCTGATTT CTTCTTCTAC TAATGTGGCT 
GTTGACAGAG TACTTCTTGG GCTTCTCAGT CTTGGATTTG AAAACTTTAT CAGAGTTGGG 
AGTGTTAGGA AGATTGCCAA ACCAATTTTA CCTTATAGCT TGCATGCTGG CTCAGAAAAT 
GAAAGTGAAC AGTTAAAAGA ACTACATGCA CTAATGAAAG AAGACCTGAC TCCTACGGAA 
AGAGTCTATG TGAGAAAAAG CATTGAGCAG CATAAACTGG GGACCAATAG AACCCTGCTG 
AAGCAGGTTC GAGTAGTTGG AGTTACCTGT GCAGCCTGCC CATTCCCATG CATGAATGAT 
CTTAAATTTC CTGTAGTTGT GCTGGATGAG TGTAGTCAGA TAACTGAACC GGCCTCTCTC 
CTTCCCATTG CAAGGTTTGA GTGTGAAAAG CTGATTCTTG TTGGGGATCC CAAACAGCTA 
CCTCCTACTA TTCAGGGTTC TGATGCAGCT CATGAAAATG GATTGGAACA AACTCTTTTT 
GATCGACTTT GCTTAATGGG TCACAAGCCA ATTCTATTGA GAACTCAATA CCGTTGTCAT 
CCTGCAATCA GTGCTATTGC TAATGATCTG TTTTACAAAG GAGCCCTCAT GAATGGTGTA 
ACAGAAATAG AGCGGAGCCC TTTATTGGAA TGGCTACCAA CCCTGTGTTT TTATAATGTT 
AAAGGACTAG AACAGATAGA AAGAGATAAC AGCTTTCATA ATGTGGCAGA AGCTACGTTT 
ACACTCAAGC TGATTCAATC ACTGATTGCA AGTGGAATAG CAGGCTCTAT GATTGGTGTG 
ATAACATTAT ACAAATCCCA GATGTACAAG CTTTGTCATT TACTCAGTGC TGTGGACTTT 
CACCATCCTG ATATTAAAAC TGTGCAGGTG TCCACAGTAG ATGCTTTTCA GGGAGCTGAA 
AAGGAGATCA TTATTCTGTC CTGTGTAAGG ACAAGACAAG TAGGATTCAT TGATTCAGAA 
AAAAGAATGA ATGTTGCATT GACTAGAGGA AAGAGGCATT TGTTGATTGT GGGAAATTTA 
GCCTGTTTGA GGAAAAATCA ACTTTGGGGA CGAGTGATCC AACACTGCGA AGGAAGGGAA 
GATGGATTGC AACATGCAAA CCAGTATGAA CCACAGCTGA ACCATCTCCT TAAAGATTAT 
TTTGAAAAAC AAGTGGAAGA AAAACAGAAG AAAAAGAGTG AAAAAGAGAA ATCTAAAGAT 
AAATCTCATT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863858.1
CDS	230..6484
Translation	MESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKVKPGDDLESDRYLITVEEVKVAGAIGIVKQNVNKEAPELNSRTFISSGRSLGCQPSGLKRKFTGFQGPRQVPKKMVIMESGESAASHEAKKTGPTIFSPFCSMPPLFPTVGKKDVNNILADPENIVTYKNRERNAMDFSSVFSPSFQINPEVLCEENYFCSPVNSGNKLSDSLLTNEPVKRDSLASHYSGVSQNIRSKAQILALLKSESSSSCEELNSEMTEHFPQKQPQGSLKIATKPKYLIQQEECAEMKSTENLYYQHQSENTMRNKSRWAMYLSSQSSPIHSSTVDGNDTERKPKAQEDDVNSNLKDLSLQKIIQFVETYAEERKKYNVDQSVGNNDPSWNQEVKLEIPSFNESSSLQVTCSSAENDGILSESDIQEDNKIPFNQNDKGCIKGSVLIKENAQEVNTCGTLEKEYEQSESSLPELKHLQIESSNNSRISDDITDMISESKMDNESLNSIHESLSNVTQPFLEVTFNLNNFETSDTEEESQESNKISQDSESWVKDILVNDGNSCFQKRSENTNCEEIEGEHLPFLTSVSDKPTVTFPVKETLPSQFCDKTYVGFDMGICKTENTGKEIEEYSDTLSNFESFKWTDAVYGDNKEDANKPIQEVRINYDFALPPNKSKGINMNLHIPHIQNQIAENSNLFSEDAQPQPFILGSDLDKNDEHVLPSTSSSDNSVQLLNTNQNHYECIALDKSNTHISNSLFYPLGKKHLISKDTEAHISEPEDLGKIRSPPPDHVEVETAREGKQYWNPRNSSELSGLVNTISILKSLCEHSTALDSLEILKKKNTVFQQGTQQTYEPDSPPEVRKPFITVVSPKSPHLHKDSQQILKEDEVELSEPLQSVQFSSSGSKEETAFQAVIPKQIERKTCDPKPVEFQGHQVKGSATSGVMVRGHSSQLGCSQFPDSTEYENFMTETPELPSTCMQIDFLQVTSPEENISTLSPVSTFSLNSRDEDFMVEFSETSLKARTLPDDLHFLNLEGMKKSRSLENENLQRLSLLSRTQVPLITLPRTDGPPDLDSHSYMINSNTYESSGSPMLNLCEKSAVLSFSIEPEDQNETFFSEESREVNPGDVSLNNISTQSKWLKYQNTSQCNVATPNRVDKRITDGFFAEAVSGMHFRDTSERQSDAVNESSLDSVHLQMIKGMLYQQRQDFSSQDSVSRKKVLSLNLKQTSKTEEIKNVLGGSTCYNYSVKDLQEISGSELCFPSGQKIKSAYLPQRQIHIPAVFQSPAHYKQTFTSCLIEHLNILLFGLAQNLQKALSKVDISFYTSLKGEKLKNAENNVPSCHHSQPAKLVMVKKEGPNKGRLFYTCDGPKADRCKFFKWLEDVTPGYSTQEGARPGMVLSDIKSIGLYLRSQKIPLYEECQLLVRKGFDFQRKQYGKLKKFTTVNPEFYNEPKTKLYLKLSRKERSSAYSKNDLWVVSKTLDFELDTFIACSAFFGPSSINEIEILPLKGYFPSNWPTNMVVHALLVCNASTELTTLKNIQDYFNPATLPLTQYLLTTSSPTIVSNKRVSKRKFIPPAFTNVSTKFELLSLGATLKLASELIQVHKLNKDQATALIQIAQMMASHESIEEVKELQTHTFPITIIHGVFGAGKSYLLAVVILFFVQLFEKSEAPTIGNARPWKLLISSSTNVAVDRVLLGLLSLGFENFIRVGSVRKIAKPILPYSLHAGSENESEQLKELHALMKEDLTPTERVYVRKSIEQHKLGTNRTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPAISAIANDLFYKGALMNGVTEIERSPLLEWLPTLCFYNVKGLEQIERDNSFHNVAEATFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAVDFHHPDIKTVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGKRHLLIVGNLACLRKNQLWGRVIQHCEGREDGLQHANQYEPQLNHLLKDYFEKQVEEKQKKKSEKEKSKDKSHS

Target ORF information:

RefSeq Version	XM_017008369.2
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008369.2

ORF Insert Sequence:

ATGGAAAGCC AAGAATTTAT TGTTCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGTGAAACC TGGAGATGAC 
TTAGAAAGTG ATCGATACTT AATCACAGTT GAAGAGGTTA AAGTTGCTGG AGCCATAGGT 
ATTGTTAAGC AGAATGTCAA TAAAGAAGCA CCAGAGTTAA ATTCAAGGAC ATTTATATCC 
TCTGGCCGAT CTCTTGGATG TCAGCCCTCT GGCTTAAAAA GGAAGTTTAC TGGTTTTCAA 
GGACCACGTC AGGTTCCAAA GAAAATGGTT ATTATGGAAA GTGGTGAATC AGCTGCATCA 
CATGAGGCTA AGAAAACTGG CCCTACTATT TTTTCTCCAT TCTGCAGCAT GCCTCCTTTG 
TTTCCTACTG TTGGCAAGAA AGATGTAAAT AATATACTGG CAGACCCTGA GAACATTGTG 
ACTTACAAGA ACAGGGAGAG AAATGCCATG GATTTTTCTT CGGTTTTTTC TCCATCCTTC 
CAGATTAACC CAGAAGTGCT GTGTGAAGAA AATTATTTTT GCTCACCTGT CAATTCTGGA 
AATAAGCTTT CAGACTCTTT ACTGACCAAT GAGCCTGTGA AAAGAGATAG TTTGGCATCT 
CACTATTCAG GAGTTTCACA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTGAAG 
TCCGAATCAT CTAGTTCATG TGAGGAACTA AATTCTGAGA TGACAGAGCA TTTTCCTCAA 
AAACAACCAC AAGGAAGTTT AAAAATTGCT ACTAAACCAA AGTACCTAAT TCAACAGGAA 
GAGTGTGCTG AGATGAAGAG CACAGAAAAT TTATACTACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TATACATTCT 
TCTACTGTAG ATGGGAATGA TACAGAAAGG AAACCCAAGG CCCAGGAAGA TGATGTAAAT 
TCTAATTTGA AAGACCTTTC ATTACAAAAA ATTATACAGT TCGTTGAAAC GTATGCTGAA 
GAGAGGAAAA AGTATAATGT AGACCAGTCA GTCGGTAATA ATGATCCATC CTGGAATCAG 
GAAGTAAAAT TAGAAATTCC TTCATTCAAT GAAAGCAGTA GCTTACAGGT TACTTGTAGC 
AGTGCAGAAA ATGATGGTAT ATTATCAGAA TCTGACATTC AAGAAGATAA TAAAATACCT 
TTTAATCAAA ATGACAAGGG GTGCATTAAA GGATCAGTTC TCATTAAAGA AAATGCTCAG 
GAGGTAAATA CATGTGGAAC ACTGGAAAAG GAGTATGAAC AATCAGAGTC ATCTCTGCCA 
GAACTGAAAC ATCTCCAAAT TGAATCTAGT AATAATTCTA GGATCTCTGA TGACATTACA 
GACATGATTT CTGAAAGTAA AATGGATAAT GAAAGTCTTA ATAGTATTCA TGAATCTCTG 
AGTAATGTAA CACAACCATT TTTGGAGGTA ACTTTTAATC TGAACAATTT TGAGACCAGT 
GACACTGAGG AGGAATCACA GGAAAGCAAC AAAATTTCCC AGGATTCAGA GAGTTGGGTA 
AAGGACATTT TGGTTAATGA TGGCAATTCA TGTTTTCAAA AGAGGTCTGA GAATACAAAC 
TGTGAAGAGA TTGAGGGTGA ACATTTGCCA TTCTTAACAT CTGTTAGTGA CAAACCTACA 
GTGACATTTC CTGTTAAAGA GACTCTGCCA TCACAGTTTT GTGATAAAAC TTATGTGGGT 
TTTGACATGG GAATATGTAA AACTGAAAAC ACAGGAAAAG AAATAGAGGA GTATAGTGAC 
ACATTAAGCA ATTTTGAATC TTTCAAGTGG ACTGATGCTG TATACGGAGA TAATAAAGAA 
GATGCTAATA AACCTATTCA AGAAGTCAGA ATTAACTATG ATTTTGCTTT ACCCCCGAAT 
AAATCTAAAG GTATAAACAT GAATTTACAT ATTCCTCATA TTCAAAACCA GATTGCTGAA 
AACAGTAATC TATTTTCAGA AGATGCTCAA CCTCAGCCTT TTATTTTGGG AAGTGACTTA 
GACAAAAATG ATGAACATGT TTTACCCTCA ACTTCTAGTA GTGACAACAG TGTCCAACTA 
TTAAATACCA ATCAGAATCA CTATGAATGT ATTGCACTTG ATAAATCAAA TACCCACATT 
TCCAATTCTT TGTTTTACCC ACTGGGAAAA AAGCATCTTA TTTCCAAAGA CACAGAAGCA 
CATATATCTG AACCTGAAGA TTTGGGAAAG ATTAGAAGTC CACCCCCTGA CCATGTTGAG 
GTTGAAACTG CCAGAGAAGG CAAACAATAC TGGAATCCTA GAAATTCTTC AGAACTTTCT 
GGATTAGTAA ATACCATTTC TATTTTAAAG TCGCTATGTG AACACAGTAC TGCTTTAGAC 
AGCTTGGAAA TATTGAAAAA GAAAAATACT GTCTTTCAGC AAGGAACTCA ACAGACGTAT 
GAGCCAGATA GCCCTCCTGA AGTGAGGAAA CCATTTATTA CAGTAGTTTC ACCAAAGTCT 
CCTCATCTGC ACAAGGATTC TCAACAGATA CTAAAAGAAG ATGAAGTTGA ACTAAGTGAA 
CCACTTCAGT CTGTGCAGTT CTCTTCCTCG GGAAGTAAAG AAGAGACTGC TTTTCAAGCT 
GTTATTCCTA AACAAATAGA GAGAAAAACC TGTGACCCTA AGCCTGTTGA GTTTCAAGGA 
CATCAAGTAA AAGGATCAGC TACTAGTGGT GTAATGGTCA GAGGACACAG CTCACAGCTA 
GGATGCAGTC AGTTTCCAGA TAGCACTGAG TATGAAAACT TTATGACAGA GACACCAGAG 
TTACCTAGCA CGTGTATGCA GATTGACTTC TTGCAGGTGA CATCACCAGA AGAAAACATC 
TCTACATTGA GCCCTGTTTC TACCTTTTCT TTGAACTCAA GAGATGAAGA CTTCATGGTA 
GAATTCTCTG AGACGTCCCT GAAAGCAAGA ACTTTACCTG ATGATCTTCA TTTTCTCAAC 
TTGGAGGGGA TGAAAAAATC CCGTTCTCTG GAGAATGAGA ACCTTCAAAG GCTTTCATTA 
TTAAGTAGAA CCCAGGTTCC ACTTATTACT TTGCCACGTA CTGATGGGCC ACCTGACTTA 
GACTCTCATT CGTATATGAT CAACTCTAAC ACATACGAGT CTTCTGGCTC CCCCATGCTC 
AATTTGTGTG AAAAGTCAGC AGTTCTTTCG TTTAGCATTG AGCCTGAGGA CCAAAATGAA 
ACCTTTTTCT CTGAAGAATC TAGGGAAGTG AATCCAGGGG ATGTTTCACT TAATAATATA 
TCTACTCAGA GCAAGTGGCT GAAATATCAA AACACATCCC AATGCAACGT GGCTACTCCA 
AACAGAGTTG ATAAGAGAAT AACTGATGGC TTCTTTGCTG AGGCTGTTTC TGGGATGCAT 
TTTAGAGACA CAAGTGAAAG ACAGAGTGAT GCTGTCAATG AAAGCTCTTT AGACTCTGTG 
CATTTGCAAA TGATAAAAGG CATGCTCTAT CAACAGCGGC AGGATTTTAG CAGTCAAGAT 
TCGGTTTCCA GAAAGAAAGT ACTTTCTCTG AATTTAAAGC AGACTTCTAA GACAGAGGAA 
ATTAAAAATG TATTAGGAGG GTCTACCTGC TACAACTACA GTGTAAAGGA TTTACAGGAG 
ATAAGTGGCT CTGAGCTGTG CTTTCCAAGT GGGCAGAAAA TAAAATCTGC TTATCTTCCC 
CAAAGGCAAA TTCACATACC AGCTGTTTTT CAGTCTCCTG CTCATTATAA GCAGACTTTC 
ACATCTTGCC TCATAGAACA TCTAAATATA TTGCTGTTTG GGTTAGCACA AAACCTGCAG 
AAAGCTCTTT CAAAAGTTGA CATATCATTT TATACATCAT TGAAGGGAGA GAAACTGAAA 
AACGCAGAAA ATAATGTACC ATCCTGCCAT CATAGTCAAC CTGCAAAACT TGTCATGGTT 
AAAAAGGAAG GTCCAAATAA GGGTCGTCTC TTTTATACAT GTGATGGACC CAAAGCTGAT 
CGATGTAAAT TCTTTAAATG GCTTGAGGAC GTGACTCCAG GATATTCAAC ACAGGAAGGA 
GCTCGACCTG GCATGGTTTT AAGTGATATT AAGAGTATTG GCTTATATTT AAGAAGTCAA 
AAGATACCAC TTTATGAGGA ATGCCAGCTT TTGGTGAGAA AAGGATTTGA TTTTCAGAGA 
AAACAGTATG GCAAACTAAA GAAGTTTACT ACTGTAAATC CTGAGTTTTA TAATGAACCA 
AAAACCAAAC TTTATCTTAA GCTAAGTCGG AAGGAAAGAT CTTCAGCTTA TAGCAAAAAT 
GATCTTTGGG TGGTTTCAAA AACCCTAGAC TTTGAGCTGG ATACTTTTAT CGCATGTAGT 
GCTTTCTTTG GACCATCATC TATCAATGAG ATAGAAATAC TGCCTTTGAA AGGCTATTTC 
CCTTCTAATT GGCCCACTAA CATGGTTGTC CATGCGTTAT TGGTTTGTAA TGCTAGCACA 
GAACTGACTA CTTTGAAAAA CATTCAGGAC TACTTTAATC CAGCTACTCT ACCTCTAACA 
CAGTACCTGT TAACAACGTC TTCGCCAACT ATAGTTAGTA ACAAAAGAGT CAGTAAGAGA 
AAATTTATCC CACCAGCCTT CACAAATGTC AGTACAAAAT TTGAACTACT CAGCCTAGGA 
GCAACATTGA AGTTAGCTAG TGAGTTGATT CAGGTACACA AGTTAAACAA GGATCAAGCT 
ACAGCTCTAA TTCAAATAGC TCAAATGATG GCATCACATG AAAGCATTGA AGAAGTGAAG 
GAACTGCAAA CTCATACCTT CCCTATCACA ATCATACATG GTGTGTTTGG AGCAGGAAAG 
AGTTACTTGC TGGCAGTGGT GATTTTGTTC TTTGTACAGC TGTTTGAAAA GAGTGAAGCT 
CCCACCATTG GAAATGCAAG GCCGTGGAAA CTTCTGATTT CTTCTTCTAC TAATGTGGCT 
GTTGACAGAG TACTTCTTGG GCTTCTCAGT CTTGGATTTG AAAACTTTAT CAGAGTTGGG 
AGTGTTAGGA AGATTGCCAA ACCAATTTTA CCTTATAGCT TGCATGCTGG CTCAGAAAAT 
GAAAGTGAAC AGTTAAAAGA ACTACATGCA CTAATGAAAG AAGACCTGAC TCCTACGGAA 
AGAGTCTATG TGAGAAAAAG CATTGAGCAG CATAAACTGG GGACCAATAG AACCCTGCTG 
AAGCAGGTTC GAGTAGTTGG AGTTACCTGT GCAGCCTGCC CATTCCCATG CATGAATGAT 
CTTAAATTTC CTGTAGTTGT GCTGGATGAG TGTAGTCAGA TAACTGAACC GGCCTCTCTC 
CTTCCCATTG CAAGGTTTGA GTGTGAAAAG CTGATTCTTG TTGGGGATCC CAAACAGCTA 
CCTCCTACTA TTCAGGGTTC TGATGCAGCT CATGAAAATG GATTGGAACA AACTCTTTTT 
GATCGACTTT GCTTAATGGG TCACAAGCCA ATTCTATTGA GAACTCAATA CCGTTGTCAT 
CCTGCAATCA GTGCTATTGC TAATGATCTG TTTTACAAAG GAGCCCTCAT GAATGGTGTA 
ACAGAAATAG AGCGGAGCCC TTTATTGGAA TGGCTACCAA CCCTGTGTTT TTATAATGTT 
AAAGGACTAG AACAGATAGA AAGAGATAAC AGCTTTCATA ATGTGGCAGA AGCTACGTTT 
ACACTCAAGC TGATTCAATC ACTGATTGCA AGTGGAATAG CAGGCTCTAT GATTGGTGTG 
ATAACATTAT ACAAATCCCA GATGTACAAG CTTTGTCATT TACTCAGTGC TGTGGACTTT 
CACCATCCTG ATATTAAAAC TGTGCAGGTG TCCACAGTAG ATGCTTTTCA GGGAGCTGAA 
AAGGAGATCA TTATTCTGTC CTGTGTAAGG ACAAGACAAG TAGGATTCAT TGATTCAGAA 
AAAAGAATGA ATGTTGCATT GACTAGAGGA AAGAGGCATT TGTTGATTGT GGGAAATTTA 
GCCTGTTTGA GGAAAAATCA ACTTTGGGGA CGAGTGATCC AACACTGCGA AGGAAGGGAA 
GATGGATTGC AACATGCAAA CCAGTATGAA CCACAGCTGA ACCATCTCCT TAAAGATTAT 
TTTGAAAAAC AAGTGGAAGA AAAACAGAAG AAAAAGAGTG AAAAAGAGAA ATCTAAAGAT 
AAATCTCATT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_011532092.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530394.1 Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X3, mRNA.

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CloneID	OHu69372
Clone ID Related Accession (Same CDS sequence)	XM_017008369.1 , XM_017008369.2 , XM_011532092.2
Accession Version	XM_011532092.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein ZGRF1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532092.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGCC AAGAATTTAT TGTTCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGTGAAACC TGGAGATGAC 
TTAGAAAGTG ATCGATACTT AATCACAGTT GAAGAGGTTA AAGTTGCTGG AGCCATAGGT 
ATTGTTAAGC AGAATGTCAA TAAAGAAGCA CCAGAGTTAA ATTCAAGGAC ATTTATATCC 
TCTGGCCGAT CTCTTGGATG TCAGCCCTCT GGCTTAAAAA GGAAGTTTAC TGGTTTTCAA 
GGACCACGTC AGGTTCCAAA GAAAATGGTT ATTATGGAAA GTGGTGAATC AGCTGCATCA 
CATGAGGCTA AGAAAACTGG CCCTACTATT TTTTCTCCAT TCTGCAGCAT GCCTCCTTTG 
TTTCCTACTG TTGGCAAGAA AGATGTAAAT AATATACTGG CAGACCCTGA GAACATTGTG 
ACTTACAAGA ACAGGGAGAG AAATGCCATG GATTTTTCTT CGGTTTTTTC TCCATCCTTC 
CAGATTAACC CAGAAGTGCT GTGTGAAGAA AATTATTTTT GCTCACCTGT CAATTCTGGA 
AATAAGCTTT CAGACTCTTT ACTGACCAAT GAGCCTGTGA AAAGAGATAG TTTGGCATCT 
CACTATTCAG GAGTTTCACA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTGAAG 
TCCGAATCAT CTAGTTCATG TGAGGAACTA AATTCTGAGA TGACAGAGCA TTTTCCTCAA 
AAACAACCAC AAGGAAGTTT AAAAATTGCT ACTAAACCAA AGTACCTAAT TCAACAGGAA 
GAGTGTGCTG AGATGAAGAG CACAGAAAAT TTATACTACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TATACATTCT 
TCTACTGTAG ATGGGAATGA TACAGAAAGG AAACCCAAGG CCCAGGAAGA TGATGTAAAT 
TCTAATTTGA AAGACCTTTC ATTACAAAAA ATTATACAGT TCGTTGAAAC GTATGCTGAA 
GAGAGGAAAA AGTATAATGT AGACCAGTCA GTCGGTAATA ATGATCCATC CTGGAATCAG 
GAAGTAAAAT TAGAAATTCC TTCATTCAAT GAAAGCAGTA GCTTACAGGT TACTTGTAGC 
AGTGCAGAAA ATGATGGTAT ATTATCAGAA TCTGACATTC AAGAAGATAA TAAAATACCT 
TTTAATCAAA ATGACAAGGG GTGCATTAAA GGATCAGTTC TCATTAAAGA AAATGCTCAG 
GAGGTAAATA CATGTGGAAC ACTGGAAAAG GAGTATGAAC AATCAGAGTC ATCTCTGCCA 
GAACTGAAAC ATCTCCAAAT TGAATCTAGT AATAATTCTA GGATCTCTGA TGACATTACA 
GACATGATTT CTGAAAGTAA AATGGATAAT GAAAGTCTTA ATAGTATTCA TGAATCTCTG 
AGTAATGTAA CACAACCATT TTTGGAGGTA ACTTTTAATC TGAACAATTT TGAGACCAGT 
GACACTGAGG AGGAATCACA GGAAAGCAAC AAAATTTCCC AGGATTCAGA GAGTTGGGTA 
AAGGACATTT TGGTTAATGA TGGCAATTCA TGTTTTCAAA AGAGGTCTGA GAATACAAAC 
TGTGAAGAGA TTGAGGGTGA ACATTTGCCA TTCTTAACAT CTGTTAGTGA CAAACCTACA 
GTGACATTTC CTGTTAAAGA GACTCTGCCA TCACAGTTTT GTGATAAAAC TTATGTGGGT 
TTTGACATGG GAATATGTAA AACTGAAAAC ACAGGAAAAG AAATAGAGGA GTATAGTGAC 
ACATTAAGCA ATTTTGAATC TTTCAAGTGG ACTGATGCTG TATACGGAGA TAATAAAGAA 
GATGCTAATA AACCTATTCA AGAAGTCAGA ATTAACTATG ATTTTGCTTT ACCCCCGAAT 
AAATCTAAAG GTATAAACAT GAATTTACAT ATTCCTCATA TTCAAAACCA GATTGCTGAA 
AACAGTAATC TATTTTCAGA AGATGCTCAA CCTCAGCCTT TTATTTTGGG AAGTGACTTA 
GACAAAAATG ATGAACATGT TTTACCCTCA ACTTCTAGTA GTGACAACAG TGTCCAACTA 
TTAAATACCA ATCAGAATCA CTATGAATGT ATTGCACTTG ATAAATCAAA TACCCACATT 
TCCAATTCTT TGTTTTACCC ACTGGGAAAA AAGCATCTTA TTTCCAAAGA CACAGAAGCA 
CATATATCTG AACCTGAAGA TTTGGGAAAG ATTAGAAGTC CACCCCCTGA CCATGTTGAG 
GTTGAAACTG CCAGAGAAGG CAAACAATAC TGGAATCCTA GAAATTCTTC AGAACTTTCT 
GGATTAGTAA ATACCATTTC TATTTTAAAG TCGCTATGTG AACACAGTAC TGCTTTAGAC 
AGCTTGGAAA TATTGAAAAA GAAAAATACT GTCTTTCAGC AAGGAACTCA ACAGACGTAT 
GAGCCAGATA GCCCTCCTGA AGTGAGGAAA CCATTTATTA CAGTAGTTTC ACCAAAGTCT 
CCTCATCTGC ACAAGGATTC TCAACAGATA CTAAAAGAAG ATGAAGTTGA ACTAAGTGAA 
CCACTTCAGT CTGTGCAGTT CTCTTCCTCG GGAAGTAAAG AAGAGACTGC TTTTCAAGCT 
GTTATTCCTA AACAAATAGA GAGAAAAACC TGTGACCCTA AGCCTGTTGA GTTTCAAGGA 
CATCAAGTAA AAGGATCAGC TACTAGTGGT GTAATGGTCA GAGGACACAG CTCACAGCTA 
GGATGCAGTC AGTTTCCAGA TAGCACTGAG TATGAAAACT TTATGACAGA GACACCAGAG 
TTACCTAGCA CGTGTATGCA GATTGACTTC TTGCAGGTGA CATCACCAGA AGAAAACATC 
TCTACATTGA GCCCTGTTTC TACCTTTTCT TTGAACTCAA GAGATGAAGA CTTCATGGTA 
GAATTCTCTG AGACGTCCCT GAAAGCAAGA ACTTTACCTG ATGATCTTCA TTTTCTCAAC 
TTGGAGGGGA TGAAAAAATC CCGTTCTCTG GAGAATGAGA ACCTTCAAAG GCTTTCATTA 
TTAAGTAGAA CCCAGGTTCC ACTTATTACT TTGCCACGTA CTGATGGGCC ACCTGACTTA 
GACTCTCATT CGTATATGAT CAACTCTAAC ACATACGAGT CTTCTGGCTC CCCCATGCTC 
AATTTGTGTG AAAAGTCAGC AGTTCTTTCG TTTAGCATTG AGCCTGAGGA CCAAAATGAA 
ACCTTTTTCT CTGAAGAATC TAGGGAAGTG AATCCAGGGG ATGTTTCACT TAATAATATA 
TCTACTCAGA GCAAGTGGCT GAAATATCAA AACACATCCC AATGCAACGT GGCTACTCCA 
AACAGAGTTG ATAAGAGAAT AACTGATGGC TTCTTTGCTG AGGCTGTTTC TGGGATGCAT 
TTTAGAGACA CAAGTGAAAG ACAGAGTGAT GCTGTCAATG AAAGCTCTTT AGACTCTGTG 
CATTTGCAAA TGATAAAAGG CATGCTCTAT CAACAGCGGC AGGATTTTAG CAGTCAAGAT 
TCGGTTTCCA GAAAGAAAGT ACTTTCTCTG AATTTAAAGC AGACTTCTAA GACAGAGGAA 
ATTAAAAATG TATTAGGAGG GTCTACCTGC TACAACTACA GTGTAAAGGA TTTACAGGAG 
ATAAGTGGCT CTGAGCTGTG CTTTCCAAGT GGGCAGAAAA TAAAATCTGC TTATCTTCCC 
CAAAGGCAAA TTCACATACC AGCTGTTTTT CAGTCTCCTG CTCATTATAA GCAGACTTTC 
ACATCTTGCC TCATAGAACA TCTAAATATA TTGCTGTTTG GGTTAGCACA AAACCTGCAG 
AAAGCTCTTT CAAAAGTTGA CATATCATTT TATACATCAT TGAAGGGAGA GAAACTGAAA 
AACGCAGAAA ATAATGTACC ATCCTGCCAT CATAGTCAAC CTGCAAAACT TGTCATGGTT 
AAAAAGGAAG GTCCAAATAA GGGTCGTCTC TTTTATACAT GTGATGGACC CAAAGCTGAT 
CGATGTAAAT TCTTTAAATG GCTTGAGGAC GTGACTCCAG GATATTCAAC ACAGGAAGGA 
GCTCGACCTG GCATGGTTTT AAGTGATATT AAGAGTATTG GCTTATATTT AAGAAGTCAA 
AAGATACCAC TTTATGAGGA ATGCCAGCTT TTGGTGAGAA AAGGATTTGA TTTTCAGAGA 
AAACAGTATG GCAAACTAAA GAAGTTTACT ACTGTAAATC CTGAGTTTTA TAATGAACCA 
AAAACCAAAC TTTATCTTAA GCTAAGTCGG AAGGAAAGAT CTTCAGCTTA TAGCAAAAAT 
GATCTTTGGG TGGTTTCAAA AACCCTAGAC TTTGAGCTGG ATACTTTTAT CGCATGTAGT 
GCTTTCTTTG GACCATCATC TATCAATGAG ATAGAAATAC TGCCTTTGAA AGGCTATTTC 
CCTTCTAATT GGCCCACTAA CATGGTTGTC CATGCGTTAT TGGTTTGTAA TGCTAGCACA 
GAACTGACTA CTTTGAAAAA CATTCAGGAC TACTTTAATC CAGCTACTCT ACCTCTAACA 
CAGTACCTGT TAACAACGTC TTCGCCAACT ATAGTTAGTA ACAAAAGAGT CAGTAAGAGA 
AAATTTATCC CACCAGCCTT CACAAATGTC AGTACAAAAT TTGAACTACT CAGCCTAGGA 
GCAACATTGA AGTTAGCTAG TGAGTTGATT CAGGTACACA AGTTAAACAA GGATCAAGCT 
ACAGCTCTAA TTCAAATAGC TCAAATGATG GCATCACATG AAAGCATTGA AGAAGTGAAG 
GAACTGCAAA CTCATACCTT CCCTATCACA ATCATACATG GTGTGTTTGG AGCAGGAAAG 
AGTTACTTGC TGGCAGTGGT GATTTTGTTC TTTGTACAGC TGTTTGAAAA GAGTGAAGCT 
CCCACCATTG GAAATGCAAG GCCGTGGAAA CTTCTGATTT CTTCTTCTAC TAATGTGGCT 
GTTGACAGAG TACTTCTTGG GCTTCTCAGT CTTGGATTTG AAAACTTTAT CAGAGTTGGG 
AGTGTTAGGA AGATTGCCAA ACCAATTTTA CCTTATAGCT TGCATGCTGG CTCAGAAAAT 
GAAAGTGAAC AGTTAAAAGA ACTACATGCA CTAATGAAAG AAGACCTGAC TCCTACGGAA 
AGAGTCTATG TGAGAAAAAG CATTGAGCAG CATAAACTGG GGACCAATAG AACCCTGCTG 
AAGCAGGTTC GAGTAGTTGG AGTTACCTGT GCAGCCTGCC CATTCCCATG CATGAATGAT 
CTTAAATTTC CTGTAGTTGT GCTGGATGAG TGTAGTCAGA TAACTGAACC GGCCTCTCTC 
CTTCCCATTG CAAGGTTTGA GTGTGAAAAG CTGATTCTTG TTGGGGATCC CAAACAGCTA 
CCTCCTACTA TTCAGGGTTC TGATGCAGCT CATGAAAATG GATTGGAACA AACTCTTTTT 
GATCGACTTT GCTTAATGGG TCACAAGCCA ATTCTATTGA GAACTCAATA CCGTTGTCAT 
CCTGCAATCA GTGCTATTGC TAATGATCTG TTTTACAAAG GAGCCCTCAT GAATGGTGTA 
ACAGAAATAG AGCGGAGCCC TTTATTGGAA TGGCTACCAA CCCTGTGTTT TTATAATGTT 
AAAGGACTAG AACAGATAGA AAGAGATAAC AGCTTTCATA ATGTGGCAGA AGCTACGTTT 
ACACTCAAGC TGATTCAATC ACTGATTGCA AGTGGAATAG CAGGCTCTAT GATTGGTGTG 
ATAACATTAT ACAAATCCCA GATGTACAAG CTTTGTCATT TACTCAGTGC TGTGGACTTT 
CACCATCCTG ATATTAAAAC TGTGCAGGTG TCCACAGTAG ATGCTTTTCA GGGAGCTGAA 
AAGGAGATCA TTATTCTGTC CTGTGTAAGG ACAAGACAAG TAGGATTCAT TGATTCAGAA 
AAAAGAATGA ATGTTGCATT GACTAGAGGA AAGAGGCATT TGTTGATTGT GGGAAATTTA 
GCCTGTTTGA GGAAAAATCA ACTTTGGGGA CGAGTGATCC AACACTGCGA AGGAAGGGAA 
GATGGATTGC AACATGCAAA CCAGTATGAA CCACAGCTGA ACCATCTCCT TAAAGATTAT 
TTTGAAAAAC AAGTGGAAGA AAAACAGAAG AAAAAGAGTG AAAAAGAGAA ATCTAAAGAT 
AAATCTCATT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530394.1
CDS	152..6406
Translation	MESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKVKPGDDLESDRYLITVEEVKVAGAIGIVKQNVNKEAPELNSRTFISSGRSLGCQPSGLKRKFTGFQGPRQVPKKMVIMESGESAASHEAKKTGPTIFSPFCSMPPLFPTVGKKDVNNILADPENIVTYKNRERNAMDFSSVFSPSFQINPEVLCEENYFCSPVNSGNKLSDSLLTNEPVKRDSLASHYSGVSQNIRSKAQILALLKSESSSSCEELNSEMTEHFPQKQPQGSLKIATKPKYLIQQEECAEMKSTENLYYQHQSENTMRNKSRWAMYLSSQSSPIHSSTVDGNDTERKPKAQEDDVNSNLKDLSLQKIIQFVETYAEERKKYNVDQSVGNNDPSWNQEVKLEIPSFNESSSLQVTCSSAENDGILSESDIQEDNKIPFNQNDKGCIKGSVLIKENAQEVNTCGTLEKEYEQSESSLPELKHLQIESSNNSRISDDITDMISESKMDNESLNSIHESLSNVTQPFLEVTFNLNNFETSDTEEESQESNKISQDSESWVKDILVNDGNSCFQKRSENTNCEEIEGEHLPFLTSVSDKPTVTFPVKETLPSQFCDKTYVGFDMGICKTENTGKEIEEYSDTLSNFESFKWTDAVYGDNKEDANKPIQEVRINYDFALPPNKSKGINMNLHIPHIQNQIAENSNLFSEDAQPQPFILGSDLDKNDEHVLPSTSSSDNSVQLLNTNQNHYECIALDKSNTHISNSLFYPLGKKHLISKDTEAHISEPEDLGKIRSPPPDHVEVETAREGKQYWNPRNSSELSGLVNTISILKSLCEHSTALDSLEILKKKNTVFQQGTQQTYEPDSPPEVRKPFITVVSPKSPHLHKDSQQILKEDEVELSEPLQSVQFSSSGSKEETAFQAVIPKQIERKTCDPKPVEFQGHQVKGSATSGVMVRGHSSQLGCSQFPDSTEYENFMTETPELPSTCMQIDFLQVTSPEENISTLSPVSTFSLNSRDEDFMVEFSETSLKARTLPDDLHFLNLEGMKKSRSLENENLQRLSLLSRTQVPLITLPRTDGPPDLDSHSYMINSNTYESSGSPMLNLCEKSAVLSFSIEPEDQNETFFSEESREVNPGDVSLNNISTQSKWLKYQNTSQCNVATPNRVDKRITDGFFAEAVSGMHFRDTSERQSDAVNESSLDSVHLQMIKGMLYQQRQDFSSQDSVSRKKVLSLNLKQTSKTEEIKNVLGGSTCYNYSVKDLQEISGSELCFPSGQKIKSAYLPQRQIHIPAVFQSPAHYKQTFTSCLIEHLNILLFGLAQNLQKALSKVDISFYTSLKGEKLKNAENNVPSCHHSQPAKLVMVKKEGPNKGRLFYTCDGPKADRCKFFKWLEDVTPGYSTQEGARPGMVLSDIKSIGLYLRSQKIPLYEECQLLVRKGFDFQRKQYGKLKKFTTVNPEFYNEPKTKLYLKLSRKERSSAYSKNDLWVVSKTLDFELDTFIACSAFFGPSSINEIEILPLKGYFPSNWPTNMVVHALLVCNASTELTTLKNIQDYFNPATLPLTQYLLTTSSPTIVSNKRVSKRKFIPPAFTNVSTKFELLSLGATLKLASELIQVHKLNKDQATALIQIAQMMASHESIEEVKELQTHTFPITIIHGVFGAGKSYLLAVVILFFVQLFEKSEAPTIGNARPWKLLISSSTNVAVDRVLLGLLSLGFENFIRVGSVRKIAKPILPYSLHAGSENESEQLKELHALMKEDLTPTERVYVRKSIEQHKLGTNRTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPAISAIANDLFYKGALMNGVTEIERSPLLEWLPTLCFYNVKGLEQIERDNSFHNVAEATFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAVDFHHPDIKTVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGKRHLLIVGNLACLRKNQLWGRVIQHCEGREDGLQHANQYEPQLNHLLKDYFEKQVEEKQKKKSEKEKSKDKSHS

Target ORF information:

RefSeq Version	XM_011532092.2
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532092.2

ORF Insert Sequence:

ATGGAAAGCC AAGAATTTAT TGTTCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGTGAAACC TGGAGATGAC 
TTAGAAAGTG ATCGATACTT AATCACAGTT GAAGAGGTTA AAGTTGCTGG AGCCATAGGT 
ATTGTTAAGC AGAATGTCAA TAAAGAAGCA CCAGAGTTAA ATTCAAGGAC ATTTATATCC 
TCTGGCCGAT CTCTTGGATG TCAGCCCTCT GGCTTAAAAA GGAAGTTTAC TGGTTTTCAA 
GGACCACGTC AGGTTCCAAA GAAAATGGTT ATTATGGAAA GTGGTGAATC AGCTGCATCA 
CATGAGGCTA AGAAAACTGG CCCTACTATT TTTTCTCCAT TCTGCAGCAT GCCTCCTTTG 
TTTCCTACTG TTGGCAAGAA AGATGTAAAT AATATACTGG CAGACCCTGA GAACATTGTG 
ACTTACAAGA ACAGGGAGAG AAATGCCATG GATTTTTCTT CGGTTTTTTC TCCATCCTTC 
CAGATTAACC CAGAAGTGCT GTGTGAAGAA AATTATTTTT GCTCACCTGT CAATTCTGGA 
AATAAGCTTT CAGACTCTTT ACTGACCAAT GAGCCTGTGA AAAGAGATAG TTTGGCATCT 
CACTATTCAG GAGTTTCACA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTGAAG 
TCCGAATCAT CTAGTTCATG TGAGGAACTA AATTCTGAGA TGACAGAGCA TTTTCCTCAA 
AAACAACCAC AAGGAAGTTT AAAAATTGCT ACTAAACCAA AGTACCTAAT TCAACAGGAA 
GAGTGTGCTG AGATGAAGAG CACAGAAAAT TTATACTACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TATACATTCT 
TCTACTGTAG ATGGGAATGA TACAGAAAGG AAACCCAAGG CCCAGGAAGA TGATGTAAAT 
TCTAATTTGA AAGACCTTTC ATTACAAAAA ATTATACAGT TCGTTGAAAC GTATGCTGAA 
GAGAGGAAAA AGTATAATGT AGACCAGTCA GTCGGTAATA ATGATCCATC CTGGAATCAG 
GAAGTAAAAT TAGAAATTCC TTCATTCAAT GAAAGCAGTA GCTTACAGGT TACTTGTAGC 
AGTGCAGAAA ATGATGGTAT ATTATCAGAA TCTGACATTC AAGAAGATAA TAAAATACCT 
TTTAATCAAA ATGACAAGGG GTGCATTAAA GGATCAGTTC TCATTAAAGA AAATGCTCAG 
GAGGTAAATA CATGTGGAAC ACTGGAAAAG GAGTATGAAC AATCAGAGTC ATCTCTGCCA 
GAACTGAAAC ATCTCCAAAT TGAATCTAGT AATAATTCTA GGATCTCTGA TGACATTACA 
GACATGATTT CTGAAAGTAA AATGGATAAT GAAAGTCTTA ATAGTATTCA TGAATCTCTG 
AGTAATGTAA CACAACCATT TTTGGAGGTA ACTTTTAATC TGAACAATTT TGAGACCAGT 
GACACTGAGG AGGAATCACA GGAAAGCAAC AAAATTTCCC AGGATTCAGA GAGTTGGGTA 
AAGGACATTT TGGTTAATGA TGGCAATTCA TGTTTTCAAA AGAGGTCTGA GAATACAAAC 
TGTGAAGAGA TTGAGGGTGA ACATTTGCCA TTCTTAACAT CTGTTAGTGA CAAACCTACA 
GTGACATTTC CTGTTAAAGA GACTCTGCCA TCACAGTTTT GTGATAAAAC TTATGTGGGT 
TTTGACATGG GAATATGTAA AACTGAAAAC ACAGGAAAAG AAATAGAGGA GTATAGTGAC 
ACATTAAGCA ATTTTGAATC TTTCAAGTGG ACTGATGCTG TATACGGAGA TAATAAAGAA 
GATGCTAATA AACCTATTCA AGAAGTCAGA ATTAACTATG ATTTTGCTTT ACCCCCGAAT 
AAATCTAAAG GTATAAACAT GAATTTACAT ATTCCTCATA TTCAAAACCA GATTGCTGAA 
AACAGTAATC TATTTTCAGA AGATGCTCAA CCTCAGCCTT TTATTTTGGG AAGTGACTTA 
GACAAAAATG ATGAACATGT TTTACCCTCA ACTTCTAGTA GTGACAACAG TGTCCAACTA 
TTAAATACCA ATCAGAATCA CTATGAATGT ATTGCACTTG ATAAATCAAA TACCCACATT 
TCCAATTCTT TGTTTTACCC ACTGGGAAAA AAGCATCTTA TTTCCAAAGA CACAGAAGCA 
CATATATCTG AACCTGAAGA TTTGGGAAAG ATTAGAAGTC CACCCCCTGA CCATGTTGAG 
GTTGAAACTG CCAGAGAAGG CAAACAATAC TGGAATCCTA GAAATTCTTC AGAACTTTCT 
GGATTAGTAA ATACCATTTC TATTTTAAAG TCGCTATGTG AACACAGTAC TGCTTTAGAC 
AGCTTGGAAA TATTGAAAAA GAAAAATACT GTCTTTCAGC AAGGAACTCA ACAGACGTAT 
GAGCCAGATA GCCCTCCTGA AGTGAGGAAA CCATTTATTA CAGTAGTTTC ACCAAAGTCT 
CCTCATCTGC ACAAGGATTC TCAACAGATA CTAAAAGAAG ATGAAGTTGA ACTAAGTGAA 
CCACTTCAGT CTGTGCAGTT CTCTTCCTCG GGAAGTAAAG AAGAGACTGC TTTTCAAGCT 
GTTATTCCTA AACAAATAGA GAGAAAAACC TGTGACCCTA AGCCTGTTGA GTTTCAAGGA 
CATCAAGTAA AAGGATCAGC TACTAGTGGT GTAATGGTCA GAGGACACAG CTCACAGCTA 
GGATGCAGTC AGTTTCCAGA TAGCACTGAG TATGAAAACT TTATGACAGA GACACCAGAG 
TTACCTAGCA CGTGTATGCA GATTGACTTC TTGCAGGTGA CATCACCAGA AGAAAACATC 
TCTACATTGA GCCCTGTTTC TACCTTTTCT TTGAACTCAA GAGATGAAGA CTTCATGGTA 
GAATTCTCTG AGACGTCCCT GAAAGCAAGA ACTTTACCTG ATGATCTTCA TTTTCTCAAC 
TTGGAGGGGA TGAAAAAATC CCGTTCTCTG GAGAATGAGA ACCTTCAAAG GCTTTCATTA 
TTAAGTAGAA CCCAGGTTCC ACTTATTACT TTGCCACGTA CTGATGGGCC ACCTGACTTA 
GACTCTCATT CGTATATGAT CAACTCTAAC ACATACGAGT CTTCTGGCTC CCCCATGCTC 
AATTTGTGTG AAAAGTCAGC AGTTCTTTCG TTTAGCATTG AGCCTGAGGA CCAAAATGAA 
ACCTTTTTCT CTGAAGAATC TAGGGAAGTG AATCCAGGGG ATGTTTCACT TAATAATATA 
TCTACTCAGA GCAAGTGGCT GAAATATCAA AACACATCCC AATGCAACGT GGCTACTCCA 
AACAGAGTTG ATAAGAGAAT AACTGATGGC TTCTTTGCTG AGGCTGTTTC TGGGATGCAT 
TTTAGAGACA CAAGTGAAAG ACAGAGTGAT GCTGTCAATG AAAGCTCTTT AGACTCTGTG 
CATTTGCAAA TGATAAAAGG CATGCTCTAT CAACAGCGGC AGGATTTTAG CAGTCAAGAT 
TCGGTTTCCA GAAAGAAAGT ACTTTCTCTG AATTTAAAGC AGACTTCTAA GACAGAGGAA 
ATTAAAAATG TATTAGGAGG GTCTACCTGC TACAACTACA GTGTAAAGGA TTTACAGGAG 
ATAAGTGGCT CTGAGCTGTG CTTTCCAAGT GGGCAGAAAA TAAAATCTGC TTATCTTCCC 
CAAAGGCAAA TTCACATACC AGCTGTTTTT CAGTCTCCTG CTCATTATAA GCAGACTTTC 
ACATCTTGCC TCATAGAACA TCTAAATATA TTGCTGTTTG GGTTAGCACA AAACCTGCAG 
AAAGCTCTTT CAAAAGTTGA CATATCATTT TATACATCAT TGAAGGGAGA GAAACTGAAA 
AACGCAGAAA ATAATGTACC ATCCTGCCAT CATAGTCAAC CTGCAAAACT TGTCATGGTT 
AAAAAGGAAG GTCCAAATAA GGGTCGTCTC TTTTATACAT GTGATGGACC CAAAGCTGAT 
CGATGTAAAT TCTTTAAATG GCTTGAGGAC GTGACTCCAG GATATTCAAC ACAGGAAGGA 
GCTCGACCTG GCATGGTTTT AAGTGATATT AAGAGTATTG GCTTATATTT AAGAAGTCAA 
AAGATACCAC TTTATGAGGA ATGCCAGCTT TTGGTGAGAA AAGGATTTGA TTTTCAGAGA 
AAACAGTATG GCAAACTAAA GAAGTTTACT ACTGTAAATC CTGAGTTTTA TAATGAACCA 
AAAACCAAAC TTTATCTTAA GCTAAGTCGG AAGGAAAGAT CTTCAGCTTA TAGCAAAAAT 
GATCTTTGGG TGGTTTCAAA AACCCTAGAC TTTGAGCTGG ATACTTTTAT CGCATGTAGT 
GCTTTCTTTG GACCATCATC TATCAATGAG ATAGAAATAC TGCCTTTGAA AGGCTATTTC 
CCTTCTAATT GGCCCACTAA CATGGTTGTC CATGCGTTAT TGGTTTGTAA TGCTAGCACA 
GAACTGACTA CTTTGAAAAA CATTCAGGAC TACTTTAATC CAGCTACTCT ACCTCTAACA 
CAGTACCTGT TAACAACGTC TTCGCCAACT ATAGTTAGTA ACAAAAGAGT CAGTAAGAGA 
AAATTTATCC CACCAGCCTT CACAAATGTC AGTACAAAAT TTGAACTACT CAGCCTAGGA 
GCAACATTGA AGTTAGCTAG TGAGTTGATT CAGGTACACA AGTTAAACAA GGATCAAGCT 
ACAGCTCTAA TTCAAATAGC TCAAATGATG GCATCACATG AAAGCATTGA AGAAGTGAAG 
GAACTGCAAA CTCATACCTT CCCTATCACA ATCATACATG GTGTGTTTGG AGCAGGAAAG 
AGTTACTTGC TGGCAGTGGT GATTTTGTTC TTTGTACAGC TGTTTGAAAA GAGTGAAGCT 
CCCACCATTG GAAATGCAAG GCCGTGGAAA CTTCTGATTT CTTCTTCTAC TAATGTGGCT 
GTTGACAGAG TACTTCTTGG GCTTCTCAGT CTTGGATTTG AAAACTTTAT CAGAGTTGGG 
AGTGTTAGGA AGATTGCCAA ACCAATTTTA CCTTATAGCT TGCATGCTGG CTCAGAAAAT 
GAAAGTGAAC AGTTAAAAGA ACTACATGCA CTAATGAAAG AAGACCTGAC TCCTACGGAA 
AGAGTCTATG TGAGAAAAAG CATTGAGCAG CATAAACTGG GGACCAATAG AACCCTGCTG 
AAGCAGGTTC GAGTAGTTGG AGTTACCTGT GCAGCCTGCC CATTCCCATG CATGAATGAT 
CTTAAATTTC CTGTAGTTGT GCTGGATGAG TGTAGTCAGA TAACTGAACC GGCCTCTCTC 
CTTCCCATTG CAAGGTTTGA GTGTGAAAAG CTGATTCTTG TTGGGGATCC CAAACAGCTA 
CCTCCTACTA TTCAGGGTTC TGATGCAGCT CATGAAAATG GATTGGAACA AACTCTTTTT 
GATCGACTTT GCTTAATGGG TCACAAGCCA ATTCTATTGA GAACTCAATA CCGTTGTCAT 
CCTGCAATCA GTGCTATTGC TAATGATCTG TTTTACAAAG GAGCCCTCAT GAATGGTGTA 
ACAGAAATAG AGCGGAGCCC TTTATTGGAA TGGCTACCAA CCCTGTGTTT TTATAATGTT 
AAAGGACTAG AACAGATAGA AAGAGATAAC AGCTTTCATA ATGTGGCAGA AGCTACGTTT 
ACACTCAAGC TGATTCAATC ACTGATTGCA AGTGGAATAG CAGGCTCTAT GATTGGTGTG 
ATAACATTAT ACAAATCCCA GATGTACAAG CTTTGTCATT TACTCAGTGC TGTGGACTTT 
CACCATCCTG ATATTAAAAC TGTGCAGGTG TCCACAGTAG ATGCTTTTCA GGGAGCTGAA 
AAGGAGATCA TTATTCTGTC CTGTGTAAGG ACAAGACAAG TAGGATTCAT TGATTCAGAA 
AAAAGAATGA ATGTTGCATT GACTAGAGGA AAGAGGCATT TGTTGATTGT GGGAAATTTA 
GCCTGTTTGA GGAAAAATCA ACTTTGGGGA CGAGTGATCC AACACTGCGA AGGAAGGGAA 
GATGGATTGC AACATGCAAA CCAGTATGAA CCACAGCTGA ACCATCTCCT TAAAGATTAT 
TTTGAAAAAC AAGTGGAAGA AAAACAGAAG AAAAAGAGTG AAAAAGAGAA ATCTAAAGAT 
AAATCTCATT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZGRF1 ( XM_011532094.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530396.1 Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X6, mRNA.

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CloneID	OHu69374
Clone ID Related Accession (Same CDS sequence)	XM_011532094.2
Accession Version	XM_011532094.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein ZGRF1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532094.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGCC AAGAATTTAT TGTTCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAATTTT ATATGATGAC 
AAAGGAGCAT GTTTGGAGAG TCTGTTTCTT AAATGCCTTG AGGTGAAACC TGGAGATGAC 
TTAGAAAGTG ATCGATACTT AATCACAGTT GAAGAGGTTA AAGTTGCTGG AGCCATAGGT 
ATTGTTAAGC AGAATGTCAA TAAAGAAGCA CCAGAGTTAA ATTCAAGGAC ATTTATATCC 
TCTGGCCGAT CTCTTGGATG TCAGCCCTCT GGCTTAAAAA GGAAGTTTAC TGGTTTTCAA 
GGACCACGTC AGGTTCCAAA GAAAATGGTT ATTATGGAAA GTGGTGAATC AGCTGCATCA 
CATGAGGCTA AGAAAACTGG CCCTACTATT TTTTCTCCAT TCTGCAGCAT GCCTCCTTTG 
TTTCCTACTG TTGGCAAGAA AGATGTAAAT AATATACTGG CAGACCCTGA GAACATTGTG 
ACTTACAAGA ACAGGGAGAG AAATGCCATG GATTTTTCTT CGGTTTTTTC TCCATCCTTC 
CAGATTAACC CAGAAGTGCT GTGTGAAGAA AATTATTTTT GCTCACCTGT CAATTCTGGA 
AATAAGCTTT CAGACTCTTT ACTGACCAAT GAGCCTGTGA AAAGAGATAG TTTGGCATCT 
CACTATTCAG GAGTTTCACA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTGAAG 
TCCGAATCAT CTAGTTCATG TGAGGAACTA AATTCTGAGA TGACAGAGCA TTTTCCTCAA 
AAACAACCAC AAGGAAGTTT AAAAATTGCT ACTAAACCAA AGTACCTAAT TCAACAGGAA 
GAGTGTGCTG AGATGAAGAG CACAGAAAAT TTATACTACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TATACATTCT 
TCTACTGTAG ATGGGAATGA TACAGAAAGG AAACCCAAGG CCCAGGAAGA TGATGTAAAT 
TCTAATTTGA AAGACCTTTC ATTACAAAAA ATTATACAGT TCGTTGAAAC GTATGCTGAA 
GAGAGGAAAA AGTATAATGT AGACCAGTCA GTCGGTAATA ATGATCCATC CTGGAATCAG 
GAAGTAAAAT TAGAAATTCC TTCATTCAAT GAAAGCAGTA GCTTACAGGT TACTTGTAGC 
AGTGCAGAAA ATGATGGTAT ATTATCAGAA TCTGACATTC AAGAAGATAA TAAAATACCT 
TTTAATCAAA ATGACAAGGG GTGCATTAAA GGATCAGTTC TCATTAAAGA AAATGCTCAG 
GAGGTAAATA CATGTGGAAC ACTGGAAAAG GAGTATGAAC AATCAGAGTC ATCTCTGCCA 
GAACTGAAAC ATCTCCAAAT TGAATCTAGT AATAATTCTA GGATCTCTGA TGACATTACA 
GACATGATTT CTGAAAGTAA AATGGATAAT GAAAGTCTTA ATAGTATTCA TGAATCTCTG 
AGTAATGTAA CACAACCATT TTTGGAGGTA ACTTTTAATC TGAACAATTT TGAGACCAGT 
GACACTGAGG AGGAATCACA GGAAAGCAAC AAAATTTCCC AGGATTCAGA GAGTTGGGTA 
AAGGACATTT TGGTTAATGA TGGCAATTCA TGTTTTCAAA AGAGGTCTGA GAATACAAAC 
TGTGAAGAGA TTGAGGGTGA ACATTTGCCA TTCTTAACAT CTGTTAGTGA CAAACCTACA 
GTGACATTTC CTGTTAAAGA GACTCTGCCA TCACAGTTTT GTGATAAAAC TTATGTGGGT 
TTTGACATGG GAATATGTAA AACTGAAAAC ACAGGAAAAG AAATAGAGGA GTATAGTGAC 
ACATTAAGCA ATTTTGAATC TTTCAAGTGG ACTGATGCTG TATACGGAGA TAATAAAGAA 
GATGCTAATA AACCTATTCA AGAAGTCAGA ATTAACTATG ATTTTGCTTT ACCCCCGAAT 
AAATCTAAAG GTATAAACAT GAATTTACAT ATTCCTCATA TTCAAAACCA GATTGCTGAA 
AACAGTAATC TATTTTCAGA AGATGCTCAA CCTCAGCCTT TTATTTTGGG AAGTGACTTA 
GACAAAAATG ATGAACATGT TTTACCCTCA ACTTCTAGTA GTGACAACAG TGTCCAACTA 
TTAAATACCA ATCAGAATCA CTATGAATGT ATTGCACTTG ATAAATCAAA TACCCACATT 
TCCAATTCTT TGTTTTACCC ACTGGGAAAA AAGCATCTTA TTTCCAAAGA CACAGAAGCA 
CATATATCTG AACCTGAAGA TTTGGGAAAG ATTAGAAGTC CACCCCCTGA CCATGTTGAG 
GTTGAAACTG CCAGAGAAGG CAAACAATAC TGGAATCCTA GAAATTCTTC AGAACTTTCT 
GGATTAGTAA ATACCATTTC TATTTTAAAG TCGCTATGTG AACACAGTAC TGCTTTAGAC 
AGCTTGGAAA TATTGAAAAA GAAAAATACT GTCTTTCAGC AAGGAACTCA ACAGACGTAT 
GAGCCAGATA GCCCTCCTGA AGTGAGGAAA CCATTTATTA CAGTAGTTTC ACCAAAGTCT 
CCTCATCTGC ACAAGGATTC TCAACAGATA CTAAAAGAAG ATGAAGTTGA ACTAAGTGAA 
CCACTTCAGT CTGTGCAGGT GACATCACCA GAAGAAAACA TCTCTACATT GAGCCCTGTT 
TCTACCTTTT CTTTGAACTC AAGAGATGAA GACTTCATGG TAGAATTCTC TGAGACGTCC 
CTGAAAGCAA GAACTTTACC TGATGATCTT CATTTTCTCA ACTTGGAGGG GATGAAAAAA 
TCCCGTTCTC TGGAGAATGA GAACCTTCAA AGGCTTTCAT TATTAAGTAG AACCCAGGTT 
CCACTTATTA CTTTGCCACG TACTGATGGG CCACCTGACT TAGACTCTCA TTCGTATATG 
ATCAACTCTA ACACATACGA GTCTTCTGGC TCCCCCATGC TCAATTTGTG TGAAAAGTCA 
GCAGTTCTTT CGTTTAGCAT TGAGCCTGAG GACCAAAATG AAACCTTTTT CTCTGAAGAA 
TCTAGGGAAG TGAATCCAGG GGATGTTTCA CTTAATAATA TATCTACTCA GAGCAAGTGG 
CTGAAATATC AAAACACATC CCAATGCAAC GTGGCTACTC CAAACAGAGT TGATAAGAGA 
ATAACTGATG GCTTCTTTGC TGAGGCTGTT TCTGGGATGC ATTTTAGAGA CACAAGTGAA 
AGACAGAGTG ATGCTGTCAA TGAAAGCTCT TTAGACTCTG TGCATTTGCA AATGATAAAA 
GGCATGCTCT ATCAACAGCG GCAGGATTTT AGCAGTCAAG ATTCGGTTTC CAGAAAGAAA 
GTACTTTCTC TGAATTTAAA GCAGACTTCT AAGACAGAGG AAATTAAAAA TGTATTAGGA 
GGGTCTACCT GCTACAACTA CAGTGTAAAG GATTTACAGG AGATAAGTGG CTCTGAGCTG 
TGCTTTCCAA GTGGGCAGAA AATAAAATCT GCTTATCTTC CCCAAAGGCA AATTCACATA 
CCAGCTGTTT TTCAGTCTCC TGCTCATTAT AAGCAGACTT TCACATCTTG CCTCATAGAA 
CATCTAAATA TATTGCTGTT TGGGTTAGCA CAAAACCTGC AGAAAGCTCT TTCAAAAGTT 
GACATATCAT TTTATACATC ATTGAAGGGA GAGAAACTGA AAAACGCAGA AAATAATGTA 
CCATCCTGCC ATCATAGTCA ACCTGCAAAA CTTGTCATGG TTAAAAAGGA AGGTCCAAAT 
AAGGGTCGTC TCTTTTATAC ATGTGATGGA CCCAAAGCTG ATCGATGTAA ATTCTTTAAA 
TGGCTTGAGG ACGTGACTCC AGGATATTCA ACACAGGAAG GAGCTCGACC TGGCATGGTT 
TTAAGTGATA TTAAGAGTAT TGGCTTATAT TTAAGAAGTC AAAAGATACC ACTTTATGAG 
GAATGCCAGC TTTTGGTGAG AAAAGGATTT GATTTTCAGA GAAAACAGTA TGGCAAACTA 
AAGAAGTTTA CTACTGTAAA TCCTGAGTTT TATAATGAAC CAAAAACCAA ACTTTATCTT 
AAGCTAAGTC GGAAGGAAAG ATCTTCAGCT TATAGCAAAA ATGATCTTTG GGTGGTTTCA 
AAAACCCTAG ACTTTGAGCT GGATACTTTT ATCGCATGTA GTGCTTTCTT TGGACCATCA 
TCTATCAATG AGATAGAAAT ACTGCCTTTG AAAGGCTATT TCCCTTCTAA TTGGCCCACT 
AACATGGTTG TCCATGCGTT ATTGGTTTGT AATGCTAGCA CAGAACTGAC TACTTTGAAA 
AACATTCAGG ACTACTTTAA TCCAGCTACT CTACCTCTAA CACAGTACCT GTTAACAACG 
TCTTCGCCAA CTATAGTTAG TAACAAAAGA GTCAGTAAGA GAAAATTTAT CCCACCAGCC 
TTCACAAATG TCAGTACAAA ATTTGAACTA CTCAGCCTAG GAGCAACATT GAAGTTAGCT 
AGTGAGTTGA TTCAGGTACA CAAGTTAAAC AAGGATCAAG CTACAGCTCT AATTCAAATA 
GCTCAAATGA TGGCATCACA TGAAAGCATT GAAGAAGTGA AGGAACTGCA AACTCATACC 
TTCCCTATCA CAATCATACA TGGTGTGTTT GGAGCAGGAA AGAGTTACTT GCTGGCAGTG 
GTGATTTTGT TCTTTGTACA GCTGTTTGAA AAGAGTGAAG CTCCCACCAT TGGAAATGCA 
AGGCCGTGGA AACTTCTGAT TTCTTCTTCT ACTAATGTGG CTGTTGACAG AGTACTTCTT 
GGGCTTCTCA GTCTTGGATT TGAAAACTTT ATCAGAGTTG GGAGTGTTAG GAAGATTGCC 
AAACCAATTT TACCTTATAG CTTGCATGCT GGCTCAGAAA ATGAAAGTGA ACAGTTAAAA 
GAACTACATG CACTAATGAA AGAAGACCTG ACTCCTACGG AAAGAGTCTA TGTGAGAAAA 
AGCATTGAGC AGCATAAACT GGGGACCAAT AGAACCCTGC TGAAGCAGGT TCGAGTAGTT 
GGAGTTACCT GTGCAGCCTG CCCATTCCCA TGCATGAATG ATCTTAAATT TCCTGTAGTT 
GTGCTGGATG AGTGTAGTCA GATAACTGAA CCGGCCTCTC TCCTTCCCAT TGCAAGGTTT 
GAGTGTGAAA AGCTGATTCT TGTTGGGGAT CCCAAACAGC TACCTCCTAC TATTCAGGGT 
TCTGATGCAG CTCATGAAAA TGGATTGGAA CAAACTCTTT TTGATCGACT TTGCTTAATG 
GGTCACAAGC CAATTCTATT GAGAACTCAA TACCGTTGTC ATCCTGCAAT CAGTGCTATT 
GCTAATGATC TGTTTTACAA AGGAGCCCTC ATGAATGGTG TAACAGAAAT AGAGCGGAGC 
CCTTTATTGG AATGGCTACC AACCCTGTGT TTTTATAATG TTAAAGGACT AGAACAGATA 
GAAAGAGATA ACAGCTTTCA TAATGTGGCA GAAGCTACGT TTACACTCAA GCTGATTCAA 
TCACTGATTG CAAGTGGAAT AGCAGGCTCT ATGATTGGTG TGATAACATT ATACAAATCC 
CAGATGTACA AGCTTTGTCA TTTACTCAGT GCTGTGGACT TTCACCATCC TGATATTAAA 
ACTGTGCAGG TGTCCACAGT AGATGCTTTT CAGGGAGCTG AAAAGGAGAT CATTATTCTG 
TCCTGTGTAA GGACAAGACA AGTAGGATTC ATTGATTCAG AAAAAAGAAT GAATGTTGCA 
TTGACTAGAG GAAAGAGGCA TTTGTTGATT GTGGGAAATT TAGCCTGTTT GAGGAAAAAT 
CAACTTTGGG GACGAGTGAT CCAACACTGC GAAGGAAGGG AAGATGGATT GCAACATGCA 
AACCAGTATG AACCACAGCT GAACCATCTC CTTAAAGATT ATTTTGAAAA ACAAGTGGAA 
GAAAAACAGA AGAAAAAGAG TGAAAAAGAG AAATCTAAAG ATAAATCTCA TTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530396.1
CDS	212..6268
Translation	MESQEFIVLYTHQKMKKSKVWQDGILKITHLGNKAILYDDKGACLESLFLKCLEVKPGDDLESDRYLITVEEVKVAGAIGIVKQNVNKEAPELNSRTFISSGRSLGCQPSGLKRKFTGFQGPRQVPKKMVIMESGESAASHEAKKTGPTIFSPFCSMPPLFPTVGKKDVNNILADPENIVTYKNRERNAMDFSSVFSPSFQINPEVLCEENYFCSPVNSGNKLSDSLLTNEPVKRDSLASHYSGVSQNIRSKAQILALLKSESSSSCEELNSEMTEHFPQKQPQGSLKIATKPKYLIQQEECAEMKSTENLYYQHQSENTMRNKSRWAMYLSSQSSPIHSSTVDGNDTERKPKAQEDDVNSNLKDLSLQKIIQFVETYAEERKKYNVDQSVGNNDPSWNQEVKLEIPSFNESSSLQVTCSSAENDGILSESDIQEDNKIPFNQNDKGCIKGSVLIKENAQEVNTCGTLEKEYEQSESSLPELKHLQIESSNNSRISDDITDMISESKMDNESLNSIHESLSNVTQPFLEVTFNLNNFETSDTEEESQESNKISQDSESWVKDILVNDGNSCFQKRSENTNCEEIEGEHLPFLTSVSDKPTVTFPVKETLPSQFCDKTYVGFDMGICKTENTGKEIEEYSDTLSNFESFKWTDAVYGDNKEDANKPIQEVRINYDFALPPNKSKGINMNLHIPHIQNQIAENSNLFSEDAQPQPFILGSDLDKNDEHVLPSTSSSDNSVQLLNTNQNHYECIALDKSNTHISNSLFYPLGKKHLISKDTEAHISEPEDLGKIRSPPPDHVEVETAREGKQYWNPRNSSELSGLVNTISILKSLCEHSTALDSLEILKKKNTVFQQGTQQTYEPDSPPEVRKPFITVVSPKSPHLHKDSQQILKEDEVELSEPLQSVQVTSPEENISTLSPVSTFSLNSRDEDFMVEFSETSLKARTLPDDLHFLNLEGMKKSRSLENENLQRLSLLSRTQVPLITLPRTDGPPDLDSHSYMINSNTYESSGSPMLNLCEKSAVLSFSIEPEDQNETFFSEESREVNPGDVSLNNISTQSKWLKYQNTSQCNVATPNRVDKRITDGFFAEAVSGMHFRDTSERQSDAVNESSLDSVHLQMIKGMLYQQRQDFSSQDSVSRKKVLSLNLKQTSKTEEIKNVLGGSTCYNYSVKDLQEISGSELCFPSGQKIKSAYLPQRQIHIPAVFQSPAHYKQTFTSCLIEHLNILLFGLAQNLQKALSKVDISFYTSLKGEKLKNAENNVPSCHHSQPAKLVMVKKEGPNKGRLFYTCDGPKADRCKFFKWLEDVTPGYSTQEGARPGMVLSDIKSIGLYLRSQKIPLYEECQLLVRKGFDFQRKQYGKLKKFTTVNPEFYNEPKTKLYLKLSRKERSSAYSKNDLWVVSKTLDFELDTFIACSAFFGPSSINEIEILPLKGYFPSNWPTNMVVHALLVCNASTELTTLKNIQDYFNPATLPLTQYLLTTSSPTIVSNKRVSKRKFIPPAFTNVSTKFELLSLGATLKLASELIQVHKLNKDQATALIQIAQMMASHESIEEVKELQTHTFPITIIHGVFGAGKSYLLAVVILFFVQLFEKSEAPTIGNARPWKLLISSSTNVAVDRVLLGLLSLGFENFIRVGSVRKIAKPILPYSLHAGSENESEQLKELHALMKEDLTPTERVYVRKSIEQHKLGTNRTLLKQVRVVGVTCAACPFPCMNDLKFPVVVLDECSQITEPASLLPIARFECEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPILLRTQYRCHPAISAIANDLFYKGALMNGVTEIERSPLLEWLPTLCFYNVKGLEQIERDNSFHNVAEATFTLKLIQSLIASGIAGSMIGVITLYKSQMYKLCHLLSAVDFHHPDIKTVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVALTRGKRHLLIVGNLACLRKNQLWGRVIQHCEGREDGLQHANQYEPQLNHLLKDYFEKQVEEKQKKKSEKEKSKDKSHS

Target ORF information:

RefSeq Version	XM_011532094.2
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger GRF-type containing 1 (ZGRF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532094.2

ORF Insert Sequence:

ATGGAAAGCC AAGAATTTAT TGTTCTATAT ACTCATCAAA AGATGAAGAA GTCAAAAGTG 
TGGCAAGATG GAATTCTGAA GATCACTCAC TTAGGAAACA AAGCAATTTT ATATGATGAC 
AAAGGAGCAT GTTTGGAGAG TCTGTTTCTT AAATGCCTTG AGGTGAAACC TGGAGATGAC 
TTAGAAAGTG ATCGATACTT AATCACAGTT GAAGAGGTTA AAGTTGCTGG AGCCATAGGT 
ATTGTTAAGC AGAATGTCAA TAAAGAAGCA CCAGAGTTAA ATTCAAGGAC ATTTATATCC 
TCTGGCCGAT CTCTTGGATG TCAGCCCTCT GGCTTAAAAA GGAAGTTTAC TGGTTTTCAA 
GGACCACGTC AGGTTCCAAA GAAAATGGTT ATTATGGAAA GTGGTGAATC AGCTGCATCA 
CATGAGGCTA AGAAAACTGG CCCTACTATT TTTTCTCCAT TCTGCAGCAT GCCTCCTTTG 
TTTCCTACTG TTGGCAAGAA AGATGTAAAT AATATACTGG CAGACCCTGA GAACATTGTG 
ACTTACAAGA ACAGGGAGAG AAATGCCATG GATTTTTCTT CGGTTTTTTC TCCATCCTTC 
CAGATTAACC CAGAAGTGCT GTGTGAAGAA AATTATTTTT GCTCACCTGT CAATTCTGGA 
AATAAGCTTT CAGACTCTTT ACTGACCAAT GAGCCTGTGA AAAGAGATAG TTTGGCATCT 
CACTATTCAG GAGTTTCACA AAACATCAGA AGCAAAGCAC AGATATTAGC TCTTCTGAAG 
TCCGAATCAT CTAGTTCATG TGAGGAACTA AATTCTGAGA TGACAGAGCA TTTTCCTCAA 
AAACAACCAC AAGGAAGTTT AAAAATTGCT ACTAAACCAA AGTACCTAAT TCAACAGGAA 
GAGTGTGCTG AGATGAAGAG CACAGAAAAT TTATACTACC AGCATCAATC AGAAAATACC 
ATGAGAAATA AAAGCCGGTG GGCCATGTAT TTATCCTCAC AGAGTTCACC TATACATTCT 
TCTACTGTAG ATGGGAATGA TACAGAAAGG AAACCCAAGG CCCAGGAAGA TGATGTAAAT 
TCTAATTTGA AAGACCTTTC ATTACAAAAA ATTATACAGT TCGTTGAAAC GTATGCTGAA 
GAGAGGAAAA AGTATAATGT AGACCAGTCA GTCGGTAATA ATGATCCATC CTGGAATCAG 
GAAGTAAAAT TAGAAATTCC TTCATTCAAT GAAAGCAGTA GCTTACAGGT TACTTGTAGC 
AGTGCAGAAA ATGATGGTAT ATTATCAGAA TCTGACATTC AAGAAGATAA TAAAATACCT 
TTTAATCAAA ATGACAAGGG GTGCATTAAA GGATCAGTTC TCATTAAAGA AAATGCTCAG 
GAGGTAAATA CATGTGGAAC ACTGGAAAAG GAGTATGAAC AATCAGAGTC ATCTCTGCCA 
GAACTGAAAC ATCTCCAAAT TGAATCTAGT AATAATTCTA GGATCTCTGA TGACATTACA 
GACATGATTT CTGAAAGTAA AATGGATAAT GAAAGTCTTA ATAGTATTCA TGAATCTCTG 
AGTAATGTAA CACAACCATT TTTGGAGGTA ACTTTTAATC TGAACAATTT TGAGACCAGT 
GACACTGAGG AGGAATCACA GGAAAGCAAC AAAATTTCCC AGGATTCAGA GAGTTGGGTA 
AAGGACATTT TGGTTAATGA TGGCAATTCA TGTTTTCAAA AGAGGTCTGA GAATACAAAC 
TGTGAAGAGA TTGAGGGTGA ACATTTGCCA TTCTTAACAT CTGTTAGTGA CAAACCTACA 
GTGACATTTC CTGTTAAAGA GACTCTGCCA TCACAGTTTT GTGATAAAAC TTATGTGGGT 
TTTGACATGG GAATATGTAA AACTGAAAAC ACAGGAAAAG AAATAGAGGA GTATAGTGAC 
ACATTAAGCA ATTTTGAATC TTTCAAGTGG ACTGATGCTG TATACGGAGA TAATAAAGAA 
GATGCTAATA AACCTATTCA AGAAGTCAGA ATTAACTATG ATTTTGCTTT ACCCCCGAAT 
AAATCTAAAG GTATAAACAT GAATTTACAT ATTCCTCATA TTCAAAACCA GATTGCTGAA 
AACAGTAATC TATTTTCAGA AGATGCTCAA CCTCAGCCTT TTATTTTGGG AAGTGACTTA 
GACAAAAATG ATGAACATGT TTTACCCTCA ACTTCTAGTA GTGACAACAG TGTCCAACTA 
TTAAATACCA ATCAGAATCA CTATGAATGT ATTGCACTTG ATAAATCAAA TACCCACATT 
TCCAATTCTT TGTTTTACCC ACTGGGAAAA AAGCATCTTA TTTCCAAAGA CACAGAAGCA 
CATATATCTG AACCTGAAGA TTTGGGAAAG ATTAGAAGTC CACCCCCTGA CCATGTTGAG 
GTTGAAACTG CCAGAGAAGG CAAACAATAC TGGAATCCTA GAAATTCTTC AGAACTTTCT 
GGATTAGTAA ATACCATTTC TATTTTAAAG TCGCTATGTG AACACAGTAC TGCTTTAGAC 
AGCTTGGAAA TATTGAAAAA GAAAAATACT GTCTTTCAGC AAGGAACTCA ACAGACGTAT 
GAGCCAGATA GCCCTCCTGA AGTGAGGAAA CCATTTATTA CAGTAGTTTC ACCAAAGTCT 
CCTCATCTGC ACAAGGATTC TCAACAGATA CTAAAAGAAG ATGAAGTTGA ACTAAGTGAA 
CCACTTCAGT CTGTGCAGGT GACATCACCA GAAGAAAACA TCTCTACATT GAGCCCTGTT 
TCTACCTTTT CTTTGAACTC AAGAGATGAA GACTTCATGG TAGAATTCTC TGAGACGTCC 
CTGAAAGCAA GAACTTTACC TGATGATCTT CATTTTCTCA ACTTGGAGGG GATGAAAAAA 
TCCCGTTCTC TGGAGAATGA GAACCTTCAA AGGCTTTCAT TATTAAGTAG AACCCAGGTT 
CCACTTATTA CTTTGCCACG TACTGATGGG CCACCTGACT TAGACTCTCA TTCGTATATG 
ATCAACTCTA ACACATACGA GTCTTCTGGC TCCCCCATGC TCAATTTGTG TGAAAAGTCA 
GCAGTTCTTT CGTTTAGCAT TGAGCCTGAG GACCAAAATG AAACCTTTTT CTCTGAAGAA 
TCTAGGGAAG TGAATCCAGG GGATGTTTCA CTTAATAATA TATCTACTCA GAGCAAGTGG 
CTGAAATATC AAAACACATC CCAATGCAAC GTGGCTACTC CAAACAGAGT TGATAAGAGA 
ATAACTGATG GCTTCTTTGC TGAGGCTGTT TCTGGGATGC ATTTTAGAGA CACAAGTGAA 
AGACAGAGTG ATGCTGTCAA TGAAAGCTCT TTAGACTCTG TGCATTTGCA AATGATAAAA 
GGCATGCTCT ATCAACAGCG GCAGGATTTT AGCAGTCAAG ATTCGGTTTC CAGAAAGAAA 
GTACTTTCTC TGAATTTAAA GCAGACTTCT AAGACAGAGG AAATTAAAAA TGTATTAGGA 
GGGTCTACCT GCTACAACTA CAGTGTAAAG GATTTACAGG AGATAAGTGG CTCTGAGCTG 
TGCTTTCCAA GTGGGCAGAA AATAAAATCT GCTTATCTTC CCCAAAGGCA AATTCACATA 
CCAGCTGTTT TTCAGTCTCC TGCTCATTAT AAGCAGACTT TCACATCTTG CCTCATAGAA 
CATCTAAATA TATTGCTGTT TGGGTTAGCA CAAAACCTGC AGAAAGCTCT TTCAAAAGTT 
GACATATCAT TTTATACATC ATTGAAGGGA GAGAAACTGA AAAACGCAGA AAATAATGTA 
CCATCCTGCC ATCATAGTCA ACCTGCAAAA CTTGTCATGG TTAAAAAGGA AGGTCCAAAT 
AAGGGTCGTC TCTTTTATAC ATGTGATGGA CCCAAAGCTG ATCGATGTAA ATTCTTTAAA 
TGGCTTGAGG ACGTGACTCC AGGATATTCA ACACAGGAAG GAGCTCGACC TGGCATGGTT 
TTAAGTGATA TTAAGAGTAT TGGCTTATAT TTAAGAAGTC AAAAGATACC ACTTTATGAG 
GAATGCCAGC TTTTGGTGAG AAAAGGATTT GATTTTCAGA GAAAACAGTA TGGCAAACTA 
AAGAAGTTTA CTACTGTAAA TCCTGAGTTT TATAATGAAC CAAAAACCAA ACTTTATCTT 
AAGCTAAGTC GGAAGGAAAG ATCTTCAGCT TATAGCAAAA ATGATCTTTG GGTGGTTTCA 
AAAACCCTAG ACTTTGAGCT GGATACTTTT ATCGCATGTA GTGCTTTCTT TGGACCATCA 
TCTATCAATG AGATAGAAAT ACTGCCTTTG AAAGGCTATT TCCCTTCTAA TTGGCCCACT 
AACATGGTTG TCCATGCGTT ATTGGTTTGT AATGCTAGCA CAGAACTGAC TACTTTGAAA 
AACATTCAGG ACTACTTTAA TCCAGCTACT CTACCTCTAA CACAGTACCT GTTAACAACG 
TCTTCGCCAA CTATAGTTAG TAACAAAAGA GTCAGTAAGA GAAAATTTAT CCCACCAGCC 
TTCACAAATG TCAGTACAAA ATTTGAACTA CTCAGCCTAG GAGCAACATT GAAGTTAGCT 
AGTGAGTTGA TTCAGGTACA CAAGTTAAAC AAGGATCAAG CTACAGCTCT AATTCAAATA 
GCTCAAATGA TGGCATCACA TGAAAGCATT GAAGAAGTGA AGGAACTGCA AACTCATACC 
TTCCCTATCA CAATCATACA TGGTGTGTTT GGAGCAGGAA AGAGTTACTT GCTGGCAGTG 
GTGATTTTGT TCTTTGTACA GCTGTTTGAA AAGAGTGAAG CTCCCACCAT TGGAAATGCA 
AGGCCGTGGA AACTTCTGAT TTCTTCTTCT ACTAATGTGG CTGTTGACAG AGTACTTCTT 
GGGCTTCTCA GTCTTGGATT TGAAAACTTT ATCAGAGTTG GGAGTGTTAG GAAGATTGCC 
AAACCAATTT TACCTTATAG CTTGCATGCT GGCTCAGAAA ATGAAAGTGA ACAGTTAAAA 
GAACTACATG CACTAATGAA AGAAGACCTG ACTCCTACGG AAAGAGTCTA TGTGAGAAAA 
AGCATTGAGC AGCATAAACT GGGGACCAAT AGAACCCTGC TGAAGCAGGT TCGAGTAGTT 
GGAGTTACCT GTGCAGCCTG CCCATTCCCA TGCATGAATG ATCTTAAATT TCCTGTAGTT 
GTGCTGGATG AGTGTAGTCA GATAACTGAA CCGGCCTCTC TCCTTCCCAT TGCAAGGTTT 
GAGTGTGAAA AGCTGATTCT TGTTGGGGAT CCCAAACAGC TACCTCCTAC TATTCAGGGT 
TCTGATGCAG CTCATGAAAA TGGATTGGAA CAAACTCTTT TTGATCGACT TTGCTTAATG 
GGTCACAAGC CAATTCTATT GAGAACTCAA TACCGTTGTC ATCCTGCAAT CAGTGCTATT 
GCTAATGATC TGTTTTACAA AGGAGCCCTC ATGAATGGTG TAACAGAAAT AGAGCGGAGC 
CCTTTATTGG AATGGCTACC AACCCTGTGT TTTTATAATG TTAAAGGACT AGAACAGATA 
GAAAGAGATA ACAGCTTTCA TAATGTGGCA GAAGCTACGT TTACACTCAA GCTGATTCAA 
TCACTGATTG CAAGTGGAAT AGCAGGCTCT ATGATTGGTG TGATAACATT ATACAAATCC 
CAGATGTACA AGCTTTGTCA TTTACTCAGT GCTGTGGACT TTCACCATCC TGATATTAAA 
ACTGTGCAGG TGTCCACAGT AGATGCTTTT CAGGGAGCTG AAAAGGAGAT CATTATTCTG 
TCCTGTGTAA GGACAAGACA AGTAGGATTC ATTGATTCAG AAAAAAGAAT GAATGTTGCA 
TTGACTAGAG GAAAGAGGCA TTTGTTGATT GTGGGAAATT TAGCCTGTTT GAGGAAAAAT 
CAACTTTGGG GACGAGTGAT CCAACACTGC GAAGGAAGGG AAGATGGATT GCAACATGCA 
AACCAGTATG AACCACAGCT GAACCATCTC CTTAAAGATT ATTTTGAAAA ACAAGTGGAA 
GAAAAACAGA AGAAAAAGAG TGAAAAAGAG AAATCTAAAG ATAAATCTCA TTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ZGRF1
Entrez Gene ID	55345
Full Name	zinc finger GRF-type containing 1
Synonyms	C4orf21
General protein information	Preferred Names zinc finger GRF-type containing 1 Names protein ZGRF1 GRF-type zinc finger domain-containing protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q25
Summary	The encoded protein contains GRF zinc finger (zf-GRF) and transmembrane domains. GRF zinc fingers are found in a number of DNA-binding proteins. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2017].

Homo sapiens (human)	ZGRF1	NP_060862.3
	LOC703497	XP_002804225.1
Mus musculus (house mouse)	4930422G04Rik	NP_932114.2
Pan troglodytes (chimpanzee)	LOC461442	XP_517401.3
Bos taurus (cattle)	ZGRF1	XP_002688121.1
Gallus gallus (chicken)	C4H4ORF21	XP_426326.4
Rattus norvegicus (Norway rat)	RGD1305834	XP_006233355.1

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ZGRF1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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