RFK cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RFK
Entrez Gene ID	55312
Full Name	riboflavin kinase
Synonyms	RIFK
General protein information	Preferred Names riboflavin kinase Names riboflavin kinase 0610038L10Rik ATP:riboflavin 5'-phosphotransferase flavokinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q21.13
Summary	Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).[supplied by OMIM, Nov 2009].
Disorder MIM:	613010

mRNA and Protein(s)

mRNA	Protein	Name
NM_018339.5	NP_060809.3	riboflavin kinase
NM_018339.6	NP_060809.3	riboflavin kinase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RFK	XP_001149206.2
Bos taurus (cattle)	RFK	XP_002689713.3
Danio rerio (zebrafish)	rfk	XP_689407.3
Kluyveromyces lactis	KLLA0C15213g	XP_452882.1
Neurospora crassa	NCU03970	XP_957609.1
Mus musculus (house mouse)	Rfk	NP_062310.1
Schizosaccharomyces pombe (fission yeast)	fmn1	NP_588395.1
Oryza sativa (rice)	Os10g0464400	NP_001064794.1
Rattus norvegicus (Norway rat)	Rfk	NP_001014128.1
Caenorhabditis elegans (roundworm)	R10H10.6	NP_501922.1
Saccharomyces cerevisiae (baker's yeast)	FMN1	NP_010522.1
Homo sapiens (human)	RFK	NP_060809.3
Canis lupus familiaris (dog)	RFK	XP_005615901.1
Eremothecium gossypii	AGOS_ABL109W	NP_982838.1
Arabidopsis thaliana (thale cress)	FMN/FHY	NP_193878.2
Oryza sativa (rice)	Os10g0209300	NP_001064321.1
Xenopus tropicalis (tropical clawed frog)	rfk	NP_001016959.1
Gallus gallus (chicken)	RFK	NP_001026776.1
Drosophila melanogaster (fruit fly)	CG2846	NP_649749.2
Magnaporthe oryzae (rice blast fungus)	MGG_14853	XP_003717043.1

Gene Ontology
Bibliography

Related articles in PubMed

Variability of Gene Expression Identifies Transcriptional Regulators of Early Human Embryonic Development.
Hasegawa Y, Taylor D, Ovchinnikov DA, Wolvetang EJ, de Torrent? L, Mar JC
PLoS genetics11(8)e1005428(2015 Aug)

Death receptors 4 and 5 activate Nox1 NADPH oxidase through riboflavin kinase to induce reactive oxygen species-mediated apoptotic cell death.
Park KJ, Lee CH, Kim A, Jeong KJ, Kim CH, Kim YS
The Journal of biological chemistry287(5)3313-25(2012 Jan)

Involvement of riboflavin kinase expression in cellular sensitivity against cisplatin.
Hirano G, Izumi H, Yasuniwa Y, Shimajiri S, Ke-Yong W, Sasagiri Y, Kusaba H, Matsumoto K, Hasegawa T, Akimoto M, Akashi K, Kohno K
International journal of oncology38(4)893-902(2011 Apr)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

Riboflavin kinase couples TNF receptor 1 to NADPH oxidase.
Yazdanpanah B, Wiegmann K, Tchikov V, Krut O, Pongratz C, Schramm M, Kleinridders A, Wunderlich T, Kashkar H, Uterm?hlen O, Br?ning JC, Sch?tze S, Kr?nke M
Nature460(7259)1159-63(2009 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DR4 and DR5 have a capability to activate Nox1 by recruiting RFK, resulting in ROS-mediated apoptotic cell death in tumor cells.
Title: Death receptors 4 and 5 activate Nox1 NADPH oxidase through riboflavin kinase to induce reactive oxygen species-mediated apoptotic cell death.

levels of riboflavin kinase expression correlate well with Gleason score, known as a good indicator of patient prognosis
Title: Involvement of riboflavin kinase expression in cellular sensitivity against cisplatin.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

results suggest that TNF, through the activation of RFK, enhances the incorporation of FAD in NADPH oxidase enzymes, a critical step for the assembly and activation of NADPH oxidase
Title: Riboflavin kinase couples TNF receptor 1 to NADPH oxidase.

the structure of riboflavin kinase cocrystallized with both MgADP and FMN is reported; drastic conformational changes associated with flavin binding are observed primarily at the so-called Flap I and Flap II loop regions
Title: Ligand binding-induced conformational changes in riboflavin kinase: structural basis for the ordered mechanism.

The following RFK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RFK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10709	NM_018339.6 Latest version!	Homo sapiens riboflavin kinase (RFK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu10709	NM_018339.5	Homo sapiens riboflavin kinase (RFK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RFK ( NM_018339.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060809.3 Homo sapiens riboflavin kinase (RFK), mRNA.

Price & Availability↑

CloneID	OHu10709
Clone ID Related Accession (Same CDS sequence)	NM_018339.5 , NM_018339.6
Accession Version	NM_018339.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	riboflavin kinase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB499473.1, DA154523.1, AL391868.16, AK315734.1 and DB531307.1. On Nov 28, 2009 this sequence version replaced NM_018339.4. Sequence Note: This RefSeq uses a downstream start codon compared to that used by the vast majority of human transcript data, and as indicated in the literature. The reference genome sequence contains a polymorphism that changes the upstream ATG found in most transcripts to ATT; hence the next downstream AUG is annotated. This polymorphism is represented in the Reference SNP, rs2490582. The reference genome polymorphism results in a protein that is 7 aa shorter at the N-terminus, and is supported by homologous proteins. It is also possible that leaky scanning by ribosomes would permit translation initiation from the downstream AUG in alleles containing the upstream AUG. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.69447.1, SRR1660809.181997.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGCACC TGCCTTACTT CTGCCGGGGT CAAGTGGTGC GGGGCTTCGG CCGCGGCTCC 
AAGCAGCTGG GCATCCCCAC AGCTAATTTT CCTGAGCAAG TGGTAGATAA TCTTCCAGCT 
GATATATCCA CTGGTATTTA CTATGGTTGG GCCAGTGTTG GAAGTGGAGA TGTCCATAAG 
ATGGTGGTGA GCATAGGATG GAACCCATAT TACAAGAATA CGAAGAAGTC TATGGAAACA 
CATATCATGC ATACCTTCAA AGAGGACTTC TATGGGGAAA TCCTCAATGT GGCCATTGTT 
GGCTACCTGA GACCAGAAAA GAACTTTGAT TCTTTAGAGT CACTTATTTC AGCAATTCAA 
GGTGATATTG AAGAAGCTAA GAAACGACTA GAGTTACCAG AACATTTGAA AATCAAAGAA 
GACAATTTCT TCCAGGTTTC TAAAAGCAAA ATAATGAATG GCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060809.3
CDS	358..825
Translation	MRHLPYFCRGQVVRGFGRGSKQLGIPTANFPEQVVDNLPADISTGIYYGWASVGSGDVHKMVVSIGWNPYYKNTKKSMETHIMHTFKEDFYGEILNVAIVGYLRPEKNFDSLESLISAIQGDIEEAKKRLELPEHLKIKEDNFFQVSKSKIMNGH

Target ORF information:

RefSeq Version	NM_018339.5
Organism	Homo sapiens(human)
Definition	Homo sapiens riboflavin kinase (RFK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018339.5

ORF Insert Sequence:

ATGAGGCACC TGCCTTACTT CTGCCGGGGT CAAGTGGTGC GGGGCTTCGG CCGCGGCTCC 
AAGCAGCTGG GCATCCCCAC AGCTAATTTT CCTGAGCAAG TGGTAGATAA TCTTCCAGCT 
GATATATCCA CTGGTATTTA CTATGGTTGG GCCAGTGTTG GAAGTGGAGA TGTCCATAAG 
ATGGTGGTGA GCATAGGATG GAACCCATAT TACAAGAATA CGAAGAAGTC TATGGAAACA 
CATATCATGC ATACCTTCAA AGAGGACTTC TATGGGGAAA TCCTCAATGT GGCCATTGTT 
GGCTACCTGA GACCAGAAAA GAACTTTGAT TCTTTAGAGT CACTTATTTC AGCAATTCAA 
GGTGATATTG AAGAAGCTAA GAAACGACTA GAGTTACCAG AACATTTGAA AATCAAAGAA 
GACAATTTCT TCCAGGTTTC TAAAAGCAAA ATAATGAATG GCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFK ( NM_018339.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_060809.3 Homo sapiens riboflavin kinase (RFK), mRNA.

Price & Availability↑

CloneID	OHu10709
Clone ID Related Accession (Same CDS sequence)	NM_018339.5 , NM_018339.6
Accession Version	NM_018339.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	riboflavin kinase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB499473.1, DA154523.1, AL391868.16, AK315734.1 and DB531307.1. On Nov 23, 2018 this sequence version replaced NM_018339.5. Sequence Note: This RefSeq uses a downstream start codon compared to that used by the vast majority of human transcript data, and as indicated in the literature. The reference genome sequence contains a polymorphism that changes the upstream ATG found in most transcripts to ATT; hence the next downstream AUG is annotated. This polymorphism is represented in the Reference SNP, rs2490582. The reference genome polymorphism results in a protein that is 7 aa shorter at the N-terminus, and is supported by homologous proteins. It is also possible that leaky scanning by ribosomes would permit translation initiation from the downstream AUG in alleles containing the upstream AUG. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.240136.1, SRR1660809.160763.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000376736.6/ ENSP00000365926.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGGCACC TGCCTTACTT CTGCCGGGGT CAAGTGGTGC GGGGCTTCGG CCGCGGCTCC 
AAGCAGCTGG GCATCCCCAC AGCTAATTTT CCTGAGCAAG TGGTAGATAA TCTTCCAGCT 
GATATATCCA CTGGTATTTA CTATGGTTGG GCCAGTGTTG GAAGTGGAGA TGTCCATAAG 
ATGGTGGTGA GCATAGGATG GAACCCATAT TACAAGAATA CGAAGAAGTC TATGGAAACA 
CATATCATGC ATACCTTCAA AGAGGACTTC TATGGGGAAA TCCTCAATGT GGCCATTGTT 
GGCTACCTGA GACCAGAAAA GAACTTTGAT TCTTTAGAGT CACTTATTTC AGCAATTCAA 
GGTGATATTG AAGAAGCTAA GAAACGACTA GAGTTACCAG AACATTTGAA AATCAAAGAA 
GACAATTTCT TCCAGGTTTC TAAAAGCAAA ATAATGAATG GCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060809.3
CDS	256..723
Translation	MRHLPYFCRGQVVRGFGRGSKQLGIPTANFPEQVVDNLPADISTGIYYGWASVGSGDVHKMVVSIGWNPYYKNTKKSMETHIMHTFKEDFYGEILNVAIVGYLRPEKNFDSLESLISAIQGDIEEAKKRLELPEHLKIKEDNFFQVSKSKIMNGH

Target ORF information:

RefSeq Version	NM_018339.6
Organism	Homo sapiens(human)
Definition	Homo sapiens riboflavin kinase (RFK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018339.6

ORF Insert Sequence:

ATGAGGCACC TGCCTTACTT CTGCCGGGGT CAAGTGGTGC GGGGCTTCGG CCGCGGCTCC 
AAGCAGCTGG GCATCCCCAC AGCTAATTTT CCTGAGCAAG TGGTAGATAA TCTTCCAGCT 
GATATATCCA CTGGTATTTA CTATGGTTGG GCCAGTGTTG GAAGTGGAGA TGTCCATAAG 
ATGGTGGTGA GCATAGGATG GAACCCATAT TACAAGAATA CGAAGAAGTC TATGGAAACA 
CATATCATGC ATACCTTCAA AGAGGACTTC TATGGGGAAA TCCTCAATGT GGCCATTGTT 
GGCTACCTGA GACCAGAAAA GAACTTTGAT TCTTTAGAGT CACTTATTTC AGCAATTCAA 
GGTGATATTG AAGAAGCTAA GAAACGACTA GAGTTACCAG AACATTTGAA AATCAAAGAA 
GACAATTTCT TCCAGGTTTC TAAAAGCAAA ATAATGAATG GCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Variability of Gene Expression Identifies Transcriptional Regulators of Early Human Embryonic Development.
PLoS genetics11(8)e1005428(2015 Aug)
Hasegawa Y,Taylor D,Ovchinnikov DA,Wolvetang EJ,de Torrent? L,Mar JC

Death receptors 4 and 5 activate Nox1 NADPH oxidase through riboflavin kinase to induce reactive oxygen species-mediated apoptotic cell death.
The Journal of biological chemistry287(5)3313-25(2012 Jan)
Park KJ,Lee CH,Kim A,Jeong KJ,Kim CH,Kim YS

Involvement of riboflavin kinase expression in cellular sensitivity against cisplatin.
International journal of oncology38(4)893-902(2011 Apr)
Hirano G,Izumi H,Yasuniwa Y,Shimajiri S,Ke-Yong W,Sasagiri Y,Kusaba H,Matsumoto K,Hasegawa T,Akimoto M,Akashi K,Kohno K

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

Riboflavin kinase couples TNF receptor 1 to NADPH oxidase.
Nature460(7259)1159-63(2009 Aug)
Yazdanpanah B,Wiegmann K,Tchikov V,Krut O,Pongratz C,Schramm M,Kleinridders A,Wunderlich T,Kashkar H,Uterm?hlen O,Br?ning JC,Sch?tze S,Kr?nke M

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RFK cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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