PEF1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PEF1
Entrez Gene ID	553115
Full Name	penta-EF-hand domain containing 1
Synonyms	ABP32,PEF1A
General protein information	Preferred Names penta-EF-hand domain containing 1 Names peflin PEF protein with a long N-terminal hydrophobic domain
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p35.2
Summary	This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. The encoded protein has been shown to form a heterodimer with the programmed cell death 6 gene product and may modulate its function in Ca(2+) signaling. Alternative splicing results in multiple transcript variants and a pseudogene has been identified on chromosome 1.[provided by RefSeq, May 2010].
Disorder MIM:	610033

mRNA and Protein(s)

mRNA	Protein	Name
XM_017001680.1	XP_016857169.1	peflin isoform X2
XM_011541746.2	XP_011540048.1	peflin isoform X2
XM_017001681.1	XP_016857170.1	peflin isoform X3
XM_011541747.1	XP_011540049.1	peflin isoform X2
NM_012392.4	NP_036524.1	peflin isoform 1
NM_012392.3	NP_036524.1	peflin
NM_001359651.1	NP_001346580.1	peflin isoform 2
XM_011541745.1	XP_011540047.1	peflin isoform X1

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AT5G04170	NP_196037.2
Arabidopsis thaliana (thale cress)	AT3G10300	NP_187641.2
Oryza sativa (rice)	Os11g0140600	NP_001065711.1
Pan troglodytes (chimpanzee)	PEF1	XP_513269.2
	LOC704758	XP_001101211.1
Danio rerio (zebrafish)	pef1	NP_001003643.1
Bos taurus (cattle)	PEF1	NP_001091461.1
Mus musculus (house mouse)	Pef1	NP_080717.2
Rattus norvegicus (Norway rat)	Pef1	NP_001007652.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006246	XP_316312.3
Oryza sativa (rice)	Os12g0137100	NP_001066106.1
Xenopus tropicalis (tropical clawed frog)	pef1	XP_002938526.1
Homo sapiens (human)	PEF1	NP_036524.1
	PEF1	XP_001100003.1
Canis lupus familiaris (dog)	PEF1	XP_005617978.1
Gallus gallus (chicken)	PEF1	XP_417792.1
Drosophila melanogaster (fruit fly)	CG17765	NP_610592.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of the CUL3?Ubiquitin Ligase by a Calcium-Dependent Co-adaptor.
McGourty CA, Akopian D, Walsh C, Gorur A, Werner A, Schekman R, Bautista D, Rape M
Cell167(2)525-538.e14(2016 Oct)

Penta-EF-Hand Protein Peflin Is a Negative Regulator of ER-To-Golgi Transport.
Rayl M, Truitt M, Held A, Sargeant J, Thorsen K, Hay JC
PloS one11(6)e0157227(2016)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Toward an understanding of the protein interaction network of the human liver.
Wang J, Huo K, Ma L, Tang L, Li D, Huang X, Yuan Y, Li C, Wang W, Guan W, Chen H, Jin C, Wei J, Zhang W, Yang Y, Liu Q, Zhou Y, Zhang C, Wu Z, Xu W, Zhang Y, Liu T, Yu D, Zhang Y, Chen L, Zhu D, Zhong X, Kang L, Gan X, Yu X, Ma Q, Yan J, Zhou L, Liu Z, Zhu Y, Zhou T, He F, Yang X
Molecular systems biology7536(2011 Oct)

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This work identifies for the first time a clear steady state role for a PEF protein in ER-to-Golgi transport-peflin is a negative regulator of transport.
Title: Penta-EF-Hand Protein Peflin Is a Negative Regulator of ER-To-Golgi Transport.

peflin is stabilized by dimerization through its fifth EF-hand region
Title: Both ALG-2 and peflin, penta-EF-hand (PEF) proteins, are stabilized by dimerization through their fifth EF-hand regions.

The following PEF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PEF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu11885	NM_012392.4 Latest version!	Homo sapiens penta-EF-hand domain containing 1 (PEF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu80444	NM_001359651.1 Latest version!	Homo sapiens penta-EF-hand domain containing 1 (PEF1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu80444	XM_017001680.1 Latest version!	Homo sapiens penta-EF-hand domain containing 1 (PEF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu80444	XM_011541746.2 Latest version!	Homo sapiens penta-EF-hand domain containing 1 (PEF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu80444	XM_011541747.1 Latest version!	Homo sapiens penta-EF-hand domain containing 1 (PEF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu80443	XM_011541745.1 Latest version!	Homo sapiens penta-EF-hand domain containing 1 (PEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu90190	XM_017001681.1 Latest version!	Homo sapiens penta-EF-hand domain containing 1 (PEF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu11885	NM_012392.3	Homo sapiens penta-EF-hand domain containing 1 (PEF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PEF1 ( NM_012392.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_036524.1 Homo sapiens penta-EF-hand domain containing 1 (PEF1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu11885
Clone ID Related Accession (Same CDS sequence)	NM_012392.4 , NM_012392.3
Accession Version	NM_012392.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	peflin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC012561.1, AC114488.3 and CA307778.1. On Nov 22, 2018 this sequence version replaced NM_012392.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1163657.142956.1, SRR3476690.202122.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000373703.5/ ENSP00000362807.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCAGCT ATCCTTACCG GCAGGGCTGC CCAGGAGCTG CAGGACAAGC ACCAGGAGCC 
CCTCCGGGTA GCTACTACCC TGGACCCCCC AATAGTGGAG GGCAGTATGG TAGTGGGCTA 
CCCCCTGGTG GTGGTTATGG GGGTCCTGCC CCTGGAGGGC CTTATGGACC ACCAGCTGGT 
GGAGGGCCCT ATGGACACCC CAATCCTGGG ATGTTCCCCT CTGGAACTCC AGGAGGACCA 
TATGGCGGTG CAGCTCCCGG GGGCCCCTAT GGTCAGCCAC CTCCAAGTTC CTACGGTGCC 
CAGCAGCCTG GGCTTTATGG ACAGGGTGGC GCCCCTCCCA ATGTGGATCC TGAGGCCTAC 
TCCTGGTTCC AGTCGGTGGA CTCAGATCAC AGTGGCTATA TCTCCATGAA GGAGCTAAAG 
CAGGCCCTGG TCAACTGCAA TTGGTCTTCA TTCAATGATG AGACCTGCCT CATGATGATA 
AACATGTTTG ACAAGACCAA GTCAGGCCGC ATCGATGTCT ACGGCTTCTC AGCCCTGTGG 
AAATTCATCC AGCAGTGGAA GAACCTCTTC CAGCAGTATG ACCGGGACCG CTCGGGCTCC 
ATTAGCTACA CAGAGCTGCA GCAAGCTCTG TCCCAAATGG GCTACAACCT GAGCCCCCAG 
TTCACCCAGC TTCTGGTCTC CCGCTACTGC CCACGCTCTG CCAATCCTGC CATGCAGCTT 
GACCGCTTCA TCCAGGTGTG CACCCAGCTG CAGGTGCTGA CAGAGGCCTT CCGGGAGAAG 
GACACAGCTG TACAAGGCAA CATTCGGCTC AGCTTCGAGG ACTTCGTCAC CATGACAGCT 
TCTCGGATGC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036524.1
CDS	13..867
Translation	MASYPYRQGCPGAAGQAPGAPPGSYYPGPPNSGGQYGSGLPPGGGYGGPAPGGPYGPPAGGGPYGHPNPGMFPSGTPGGPYGGAAPGGPYGQPPPSSYGAQQPGLYGQGGAPPNVDPEAYSWFQSVDSDHSGYISMKELKQALVNCNWSSFNDETCLMMINMFDKTKSGRIDVYGFSALWKFIQQWKNLFQQYDRDRSGSISYTELQQALSQMGYNLSPQFTQLLVSRYCPRSANPAMQLDRFIQVCTQLQVLTEAFREKDTAVQGNIRLSFEDFVTMTASRML

Target ORF information:

RefSeq Version	NM_012392.4
Organism	Homo sapiens(human)
Definition	Homo sapiens penta-EF-hand domain containing 1 (PEF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012392.4

ORF Insert Sequence:

ATGGCCAGCT ATCCTTACCG GCAGGGCTGC CCAGGAGCTG CAGGACAAGC ACCAGGAGCC 
CCTCCGGGTA GCTACTACCC TGGACCCCCC AATAGTGGAG GGCAGTATGG TAGTGGGCTA 
CCCCCTGGTG GTGGTTATGG GGGTCCTGCC CCTGGAGGGC CTTATGGACC ACCAGCTGGT 
GGAGGGCCCT ATGGACACCC CAATCCTGGG ATGTTCCCCT CTGGAACTCC AGGAGGACCA 
TATGGCGGTG CAGCTCCCGG GGGCCCCTAT GGTCAGCCAC CTCCAAGTTC CTACGGTGCC 
CAGCAGCCTG GGCTTTATGG ACAGGGTGGC GCCCCTCCCA ATGTGGATCC TGAGGCCTAC 
TCCTGGTTCC AGTCGGTGGA CTCAGATCAC AGTGGCTATA TCTCCATGAA GGAGCTAAAG 
CAGGCCCTGG TCAACTGCAA TTGGTCTTCA TTCAATGATG AGACCTGCCT CATGATGATA 
AACATGTTTG ACAAGACCAA GTCAGGCCGC ATCGATGTCT ACGGCTTCTC AGCCCTGTGG 
AAATTCATCC AGCAGTGGAA GAACCTCTTC CAGCAGTATG ACCGGGACCG CTCGGGCTCC 
ATTAGCTACA CAGAGCTGCA GCAAGCTCTG TCCCAAATGG GCTACAACCT GAGCCCCCAG 
TTCACCCAGC TTCTGGTCTC CCGCTACTGC CCACGCTCTG CCAATCCTGC CATGCAGCTT 
GACCGCTTCA TCCAGGTGTG CACCCAGCTG CAGGTGCTGA CAGAGGCCTT CCGGGAGAAG 
GACACAGCTG TACAAGGCAA CATTCGGCTC AGCTTCGAGG ACTTCGTCAC CATGACAGCT 
TCTCGGATGC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEF1 ( NM_012392.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_036524.1 Homo sapiens penta-EF-hand domain containing 1 (PEF1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu11885
Clone ID Related Accession (Same CDS sequence)	NM_012392.4 , NM_012392.3
Accession Version	NM_012392.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	peflin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC301714.1, BC012561.1, AC114488.3 and CA307778.1. On May 26, 2010 this sequence version replaced NM_012392.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279847.4377.1, BC012561.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGCT ATCCTTACCG GCAGGGCTGC CCAGGAGCTG CAGGACAAGC ACCAGGAGCC 
CCTCCGGGTA GCTACTACCC TGGACCCCCC AATAGTGGAG GGCAGTATGG TAGTGGGCTA 
CCCCCTGGTG GTGGTTATGG GGGTCCTGCC CCTGGAGGGC CTTATGGACC ACCAGCTGGT 
GGAGGGCCCT ATGGACACCC CAATCCTGGG ATGTTCCCCT CTGGAACTCC AGGAGGACCA 
TATGGCGGTG CAGCTCCCGG GGGCCCCTAT GGTCAGCCAC CTCCAAGTTC CTACGGTGCC 
CAGCAGCCTG GGCTTTATGG ACAGGGTGGC GCCCCTCCCA ATGTGGATCC TGAGGCCTAC 
TCCTGGTTCC AGTCGGTGGA CTCAGATCAC AGTGGCTATA TCTCCATGAA GGAGCTAAAG 
CAGGCCCTGG TCAACTGCAA TTGGTCTTCA TTCAATGATG AGACCTGCCT CATGATGATA 
AACATGTTTG ACAAGACCAA GTCAGGCCGC ATCGATGTCT ACGGCTTCTC AGCCCTGTGG 
AAATTCATCC AGCAGTGGAA GAACCTCTTC CAGCAGTATG ACCGGGACCG CTCGGGCTCC 
ATTAGCTACA CAGAGCTGCA GCAAGCTCTG TCCCAAATGG GCTACAACCT GAGCCCCCAG 
TTCACCCAGC TTCTGGTCTC CCGCTACTGC CCACGCTCTG CCAATCCTGC CATGCAGCTT 
GACCGCTTCA TCCAGGTGTG CACCCAGCTG CAGGTGCTGA CAGAGGCCTT CCGGGAGAAG 
GACACAGCTG TACAAGGCAA CATTCGGCTC AGCTTCGAGG ACTTCGTCAC CATGACAGCT 
TCTCGGATGC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036524.1
CDS	374..1228
Translation	MASYPYRQGCPGAAGQAPGAPPGSYYPGPPNSGGQYGSGLPPGGGYGGPAPGGPYGPPAGGGPYGHPNPGMFPSGTPGGPYGGAAPGGPYGQPPPSSYGAQQPGLYGQGGAPPNVDPEAYSWFQSVDSDHSGYISMKELKQALVNCNWSSFNDETCLMMINMFDKTKSGRIDVYGFSALWKFIQQWKNLFQQYDRDRSGSISYTELQQALSQMGYNLSPQFTQLLVSRYCPRSANPAMQLDRFIQVCTQLQVLTEAFREKDTAVQGNIRLSFEDFVTMTASRML

Target ORF information:

RefSeq Version	NM_012392.3
Organism	Homo sapiens(human)
Definition	Homo sapiens penta-EF-hand domain containing 1 (PEF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012392.3

ORF Insert Sequence:

ATGGCCAGCT ATCCTTACCG GCAGGGCTGC CCAGGAGCTG CAGGACAAGC ACCAGGAGCC 
CCTCCGGGTA GCTACTACCC TGGACCCCCC AATAGTGGAG GGCAGTATGG TAGTGGGCTA 
CCCCCTGGTG GTGGTTATGG GGGTCCTGCC CCTGGAGGGC CTTATGGACC ACCAGCTGGT 
GGAGGGCCCT ATGGACACCC CAATCCTGGG ATGTTCCCCT CTGGAACTCC AGGAGGACCA 
TATGGCGGTG CAGCTCCCGG GGGCCCCTAT GGTCAGCCAC CTCCAAGTTC CTACGGTGCC 
CAGCAGCCTG GGCTTTATGG ACAGGGTGGC GCCCCTCCCA ATGTGGATCC TGAGGCCTAC 
TCCTGGTTCC AGTCGGTGGA CTCAGATCAC AGTGGCTATA TCTCCATGAA GGAGCTAAAG 
CAGGCCCTGG TCAACTGCAA TTGGTCTTCA TTCAATGATG AGACCTGCCT CATGATGATA 
AACATGTTTG ACAAGACCAA GTCAGGCCGC ATCGATGTCT ACGGCTTCTC AGCCCTGTGG 
AAATTCATCC AGCAGTGGAA GAACCTCTTC CAGCAGTATG ACCGGGACCG CTCGGGCTCC 
ATTAGCTACA CAGAGCTGCA GCAAGCTCTG TCCCAAATGG GCTACAACCT GAGCCCCCAG 
TTCACCCAGC TTCTGGTCTC CCGCTACTGC CCACGCTCTG CCAATCCTGC CATGCAGCTT 
GACCGCTTCA TCCAGGTGTG CACCCAGCTG CAGGTGCTGA CAGAGGCCTT CCGGGAGAAG 
GACACAGCTG TACAAGGCAA CATTCGGCTC AGCTTCGAGG ACTTCGTCAC CATGACAGCT 
TCTCGGATGC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEF1 ( NM_001359651.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001346580.1 Homo sapiens penta-EF-hand domain containing 1 (PEF1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu80444
Clone ID Related Accession (Same CDS sequence)	XM_017001680.1 , XM_011541746.2 , XM_011541747.1 , NM_001359651.1
Accession Version	NM_001359651.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Homo sapiens(human)
Product	peflin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB024828.1, BI826532.1, AC114488.3, BC012561.1 and CA307778.1. On Jan 10, 2018 this sequence version replaced NR_033688.1. Transcript Variant: This variant (3) differs in the 5' UTR and initiates translation at a downstream start codon compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.249131.1, SRR1803615.153058.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCCCCT CTGGAACTCC AGGAGGACCA TATGGCGGTG CAGCTCCCGG GGGCCCCTAT 
GGTCAGCCAC CTCCAAGTTC CTACGGTGCC CAGCAGCCTG GGCTTTATGG ACAGGGTGGC 
GCCCCTCCCA ATGTGGATCC TGAGGCCTAC TCCTGGTTCC AGTCGGTGGA CTCAGATCAC 
AGTGGCTATA TCTCCATGAA GGAGCTAAAG CAGGCCCTGG TCAACTGCAA TTGGTCTTCA 
TTCAATGATG AGACCTGCCT CATGATGATA AACATGTTTG ACAAGACCAA GTCAGGCCGC 
ATCGATGTCT ACGGCTTCTC AGCCCTGTGG AAATTCATCC AGCAGTGGAA GAACCTCTTC 
CAGCAGTATG ACCGGGACCG CTCGGGCTCC ATTAGCTACA CAGAGCTGCA GCAAGCTCTG 
TCCCAAATGG GCTACAACCT GAGCCCCCAG TTCACCCAGC TTCTGGTCTC CCGCTACTGC 
CCACGCTCTG CCAATCCTGC CATGCAGCTT GACCGCTTCA TCCAGGTGTG CACCCAGCTG 
CAGGTGCTGA CAGAGGCCTT CCGGGAGAAG GACACAGCTG TACAAGGCAA CATTCGGCTC 
AGCTTCGAGG ACTTCGTCAC CATGACAGCT TCTCGGATGC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346580.1
CDS	425..1069
Translation	MFPSGTPGGPYGGAAPGGPYGQPPPSSYGAQQPGLYGQGGAPPNVDPEAYSWFQSVDSDHSGYISMKELKQALVNCNWSSFNDETCLMMINMFDKTKSGRIDVYGFSALWKFIQQWKNLFQQYDRDRSGSISYTELQQALSQMGYNLSPQFTQLLVSRYCPRSANPAMQLDRFIQVCTQLQVLTEAFREKDTAVQGNIRLSFEDFVTMTASRML

Target ORF information:

RefSeq Version	NM_001359651.1
Organism	Homo sapiens(human)
Definition	Homo sapiens penta-EF-hand domain containing 1 (PEF1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359651.1

ORF Insert Sequence:

ATGTTCCCCT CTGGAACTCC AGGAGGACCA TATGGCGGTG CAGCTCCCGG GGGCCCCTAT 
GGTCAGCCAC CTCCAAGTTC CTACGGTGCC CAGCAGCCTG GGCTTTATGG ACAGGGTGGC 
GCCCCTCCCA ATGTGGATCC TGAGGCCTAC TCCTGGTTCC AGTCGGTGGA CTCAGATCAC 
AGTGGCTATA TCTCCATGAA GGAGCTAAAG CAGGCCCTGG TCAACTGCAA TTGGTCTTCA 
TTCAATGATG AGACCTGCCT CATGATGATA AACATGTTTG ACAAGACCAA GTCAGGCCGC 
ATCGATGTCT ACGGCTTCTC AGCCCTGTGG AAATTCATCC AGCAGTGGAA GAACCTCTTC 
CAGCAGTATG ACCGGGACCG CTCGGGCTCC ATTAGCTACA CAGAGCTGCA GCAAGCTCTG 
TCCCAAATGG GCTACAACCT GAGCCCCCAG TTCACCCAGC TTCTGGTCTC CCGCTACTGC 
CCACGCTCTG CCAATCCTGC CATGCAGCTT GACCGCTTCA TCCAGGTGTG CACCCAGCTG 
CAGGTGCTGA CAGAGGCCTT CCGGGAGAAG GACACAGCTG TACAAGGCAA CATTCGGCTC 
AGCTTCGAGG ACTTCGTCAC CATGACAGCT TCTCGGATGC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEF1 ( XM_017001680.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857169.1 Homo sapiens penta-EF-hand domain containing 1 (PEF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu80444
Clone ID Related Accession (Same CDS sequence)	XM_017001680.1 , XM_011541746.2 , XM_011541747.1 , NM_001359651.1
Accession Version	XM_017001680.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	peflin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCCT CTGGAACTCC AGGAGGACCA TATGGCGGTG CAGCTCCCGG GGGCCCCTAT 
GGTCAGCCAC CTCCAAGTTC CTACGGTGCC CAGCAGCCTG GGCTTTATGG ACAGGGTGGC 
GCCCCTCCCA ATGTGGATCC TGAGGCCTAC TCCTGGTTCC AGTCGGTGGA CTCAGATCAC 
AGTGGCTATA TCTCCATGAA GGAGCTAAAG CAGGCCCTGG TCAACTGCAA TTGGTCTTCA 
TTCAATGATG AGACCTGCCT CATGATGATA AACATGTTTG ACAAGACCAA GTCAGGCCGC 
ATCGATGTCT ACGGCTTCTC AGCCCTGTGG AAATTCATCC AGCAGTGGAA GAACCTCTTC 
CAGCAGTATG ACCGGGACCG CTCGGGCTCC ATTAGCTACA CAGAGCTGCA GCAAGCTCTG 
TCCCAAATGG GCTACAACCT GAGCCCCCAG TTCACCCAGC TTCTGGTCTC CCGCTACTGC 
CCACGCTCTG CCAATCCTGC CATGCAGCTT GACCGCTTCA TCCAGGTGTG CACCCAGCTG 
CAGGTGCTGA CAGAGGCCTT CCGGGAGAAG GACACAGCTG TACAAGGCAA CATTCGGCTC 
AGCTTCGAGG ACTTCGTCAC CATGACAGCT TCTCGGATGC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857169.1
CDS	542..1186
Translation	MFPSGTPGGPYGGAAPGGPYGQPPPSSYGAQQPGLYGQGGAPPNVDPEAYSWFQSVDSDHSGYISMKELKQALVNCNWSSFNDETCLMMINMFDKTKSGRIDVYGFSALWKFIQQWKNLFQQYDRDRSGSISYTELQQALSQMGYNLSPQFTQLLVSRYCPRSANPAMQLDRFIQVCTQLQVLTEAFREKDTAVQGNIRLSFEDFVTMTASRML

Target ORF information:

RefSeq Version	XM_017001680.1
Organism	Homo sapiens(human)
Definition	Homo sapiens penta-EF-hand domain containing 1 (PEF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001680.1

ORF Insert Sequence:

ATGTTCCCCT CTGGAACTCC AGGAGGACCA TATGGCGGTG CAGCTCCCGG GGGCCCCTAT 
GGTCAGCCAC CTCCAAGTTC CTACGGTGCC CAGCAGCCTG GGCTTTATGG ACAGGGTGGC 
GCCCCTCCCA ATGTGGATCC TGAGGCCTAC TCCTGGTTCC AGTCGGTGGA CTCAGATCAC 
AGTGGCTATA TCTCCATGAA GGAGCTAAAG CAGGCCCTGG TCAACTGCAA TTGGTCTTCA 
TTCAATGATG AGACCTGCCT CATGATGATA AACATGTTTG ACAAGACCAA GTCAGGCCGC 
ATCGATGTCT ACGGCTTCTC AGCCCTGTGG AAATTCATCC AGCAGTGGAA GAACCTCTTC 
CAGCAGTATG ACCGGGACCG CTCGGGCTCC ATTAGCTACA CAGAGCTGCA GCAAGCTCTG 
TCCCAAATGG GCTACAACCT GAGCCCCCAG TTCACCCAGC TTCTGGTCTC CCGCTACTGC 
CCACGCTCTG CCAATCCTGC CATGCAGCTT GACCGCTTCA TCCAGGTGTG CACCCAGCTG 
CAGGTGCTGA CAGAGGCCTT CCGGGAGAAG GACACAGCTG TACAAGGCAA CATTCGGCTC 
AGCTTCGAGG ACTTCGTCAC CATGACAGCT TCTCGGATGC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEF1 ( XM_011541746.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540048.1 Homo sapiens penta-EF-hand domain containing 1 (PEF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu80444
Clone ID Related Accession (Same CDS sequence)	XM_017001680.1 , XM_011541746.2 , XM_011541747.1 , NM_001359651.1
Accession Version	XM_011541746.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	peflin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011541746.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCCT CTGGAACTCC AGGAGGACCA TATGGCGGTG CAGCTCCCGG GGGCCCCTAT 
GGTCAGCCAC CTCCAAGTTC CTACGGTGCC CAGCAGCCTG GGCTTTATGG ACAGGGTGGC 
GCCCCTCCCA ATGTGGATCC TGAGGCCTAC TCCTGGTTCC AGTCGGTGGA CTCAGATCAC 
AGTGGCTATA TCTCCATGAA GGAGCTAAAG CAGGCCCTGG TCAACTGCAA TTGGTCTTCA 
TTCAATGATG AGACCTGCCT CATGATGATA AACATGTTTG ACAAGACCAA GTCAGGCCGC 
ATCGATGTCT ACGGCTTCTC AGCCCTGTGG AAATTCATCC AGCAGTGGAA GAACCTCTTC 
CAGCAGTATG ACCGGGACCG CTCGGGCTCC ATTAGCTACA CAGAGCTGCA GCAAGCTCTG 
TCCCAAATGG GCTACAACCT GAGCCCCCAG TTCACCCAGC TTCTGGTCTC CCGCTACTGC 
CCACGCTCTG CCAATCCTGC CATGCAGCTT GACCGCTTCA TCCAGGTGTG CACCCAGCTG 
CAGGTGCTGA CAGAGGCCTT CCGGGAGAAG GACACAGCTG TACAAGGCAA CATTCGGCTC 
AGCTTCGAGG ACTTCGTCAC CATGACAGCT TCTCGGATGC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540048.1
CDS	678..1322
Translation	MFPSGTPGGPYGGAAPGGPYGQPPPSSYGAQQPGLYGQGGAPPNVDPEAYSWFQSVDSDHSGYISMKELKQALVNCNWSSFNDETCLMMINMFDKTKSGRIDVYGFSALWKFIQQWKNLFQQYDRDRSGSISYTELQQALSQMGYNLSPQFTQLLVSRYCPRSANPAMQLDRFIQVCTQLQVLTEAFREKDTAVQGNIRLSFEDFVTMTASRML

Target ORF information:

RefSeq Version	XM_011541746.2
Organism	Homo sapiens(human)
Definition	Homo sapiens penta-EF-hand domain containing 1 (PEF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541746.2

ORF Insert Sequence:

ATGTTCCCCT CTGGAACTCC AGGAGGACCA TATGGCGGTG CAGCTCCCGG GGGCCCCTAT 
GGTCAGCCAC CTCCAAGTTC CTACGGTGCC CAGCAGCCTG GGCTTTATGG ACAGGGTGGC 
GCCCCTCCCA ATGTGGATCC TGAGGCCTAC TCCTGGTTCC AGTCGGTGGA CTCAGATCAC 
AGTGGCTATA TCTCCATGAA GGAGCTAAAG CAGGCCCTGG TCAACTGCAA TTGGTCTTCA 
TTCAATGATG AGACCTGCCT CATGATGATA AACATGTTTG ACAAGACCAA GTCAGGCCGC 
ATCGATGTCT ACGGCTTCTC AGCCCTGTGG AAATTCATCC AGCAGTGGAA GAACCTCTTC 
CAGCAGTATG ACCGGGACCG CTCGGGCTCC ATTAGCTACA CAGAGCTGCA GCAAGCTCTG 
TCCCAAATGG GCTACAACCT GAGCCCCCAG TTCACCCAGC TTCTGGTCTC CCGCTACTGC 
CCACGCTCTG CCAATCCTGC CATGCAGCTT GACCGCTTCA TCCAGGTGTG CACCCAGCTG 
CAGGTGCTGA CAGAGGCCTT CCGGGAGAAG GACACAGCTG TACAAGGCAA CATTCGGCTC 
AGCTTCGAGG ACTTCGTCAC CATGACAGCT TCTCGGATGC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEF1 ( XM_011541747.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540049.1 Homo sapiens penta-EF-hand domain containing 1 (PEF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu80444
Clone ID Related Accession (Same CDS sequence)	XM_017001680.1 , XM_011541746.2 , XM_011541747.1 , NM_001359651.1
Accession Version	XM_011541747.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	peflin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCCT CTGGAACTCC AGGAGGACCA TATGGCGGTG CAGCTCCCGG GGGCCCCTAT 
GGTCAGCCAC CTCCAAGTTC CTACGGTGCC CAGCAGCCTG GGCTTTATGG ACAGGGTGGC 
GCCCCTCCCA ATGTGGATCC TGAGGCCTAC TCCTGGTTCC AGTCGGTGGA CTCAGATCAC 
AGTGGCTATA TCTCCATGAA GGAGCTAAAG CAGGCCCTGG TCAACTGCAA TTGGTCTTCA 
TTCAATGATG AGACCTGCCT CATGATGATA AACATGTTTG ACAAGACCAA GTCAGGCCGC 
ATCGATGTCT ACGGCTTCTC AGCCCTGTGG AAATTCATCC AGCAGTGGAA GAACCTCTTC 
CAGCAGTATG ACCGGGACCG CTCGGGCTCC ATTAGCTACA CAGAGCTGCA GCAAGCTCTG 
TCCCAAATGG GCTACAACCT GAGCCCCCAG TTCACCCAGC TTCTGGTCTC CCGCTACTGC 
CCACGCTCTG CCAATCCTGC CATGCAGCTT GACCGCTTCA TCCAGGTGTG CACCCAGCTG 
CAGGTGCTGA CAGAGGCCTT CCGGGAGAAG GACACAGCTG TACAAGGCAA CATTCGGCTC 
AGCTTCGAGG ACTTCGTCAC CATGACAGCT TCTCGGATGC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540049.1
CDS	553..1197
Translation	MFPSGTPGGPYGGAAPGGPYGQPPPSSYGAQQPGLYGQGGAPPNVDPEAYSWFQSVDSDHSGYISMKELKQALVNCNWSSFNDETCLMMINMFDKTKSGRIDVYGFSALWKFIQQWKNLFQQYDRDRSGSISYTELQQALSQMGYNLSPQFTQLLVSRYCPRSANPAMQLDRFIQVCTQLQVLTEAFREKDTAVQGNIRLSFEDFVTMTASRML

Target ORF information:

RefSeq Version	XM_011541747.1
Organism	Homo sapiens(human)
Definition	Homo sapiens penta-EF-hand domain containing 1 (PEF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541747.1

ORF Insert Sequence:

ATGTTCCCCT CTGGAACTCC AGGAGGACCA TATGGCGGTG CAGCTCCCGG GGGCCCCTAT 
GGTCAGCCAC CTCCAAGTTC CTACGGTGCC CAGCAGCCTG GGCTTTATGG ACAGGGTGGC 
GCCCCTCCCA ATGTGGATCC TGAGGCCTAC TCCTGGTTCC AGTCGGTGGA CTCAGATCAC 
AGTGGCTATA TCTCCATGAA GGAGCTAAAG CAGGCCCTGG TCAACTGCAA TTGGTCTTCA 
TTCAATGATG AGACCTGCCT CATGATGATA AACATGTTTG ACAAGACCAA GTCAGGCCGC 
ATCGATGTCT ACGGCTTCTC AGCCCTGTGG AAATTCATCC AGCAGTGGAA GAACCTCTTC 
CAGCAGTATG ACCGGGACCG CTCGGGCTCC ATTAGCTACA CAGAGCTGCA GCAAGCTCTG 
TCCCAAATGG GCTACAACCT GAGCCCCCAG TTCACCCAGC TTCTGGTCTC CCGCTACTGC 
CCACGCTCTG CCAATCCTGC CATGCAGCTT GACCGCTTCA TCCAGGTGTG CACCCAGCTG 
CAGGTGCTGA CAGAGGCCTT CCGGGAGAAG GACACAGCTG TACAAGGCAA CATTCGGCTC 
AGCTTCGAGG ACTTCGTCAC CATGACAGCT TCTCGGATGC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEF1 ( XM_011541745.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540047.1 Homo sapiens penta-EF-hand domain containing 1 (PEF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu80443
Clone ID Related Accession (Same CDS sequence)	XM_011541745.1
Accession Version	XM_011541745.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	peflin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCAGG GCTGCCCAGG AGCTGCAGGA CAAGCACCAG GAGCCCCTCC GGGTAGCTAC 
TACCCTGGAC CCCCCAATAG TGGAGGGCAG TATGGTAGTG GGCTACCCCC TGGTGGTGGT 
TATGGGGGTC CTGCCCCTGG AGGGCCTTAT GGACCACCAG CTGGTGGAGG GCCCTATGGA 
CACCCCAATC CTGGGATGTT CCCCTCTGGA ACTCCAGGAG GACCATATGG CGGTGCAGCT 
CCCGGGGGCC CCTATGGTCA GCCACCTCCA AGTTCCTACG GTGCCCAGCA GCCTGGGCTT 
TATGGACAGG GTGGCGCCCC TCCCAATGTG GATCCTGAGG CCTACTCCTG GTTCCAGTCG 
GTGGACTCAG ATCACAGTGG CTATATCTCC ATGAAGGAGC TAAAGCAGGC CCTGGTCAAC 
TGCAATTGGT CTTCATTCAA TGATGAGACC TGCCTCATGA TGATAAACAT GTTTGACAAG 
ACCAAGTCAG GCCGCATCGA TGTCTACGGC TTCTCAGCCC TGTGGAAATT CATCCAGCAG 
TGGAAGAACC TCTTCCAGCA GTATGACCGG GACCGCTCGG GCTCCATTAG CTACACAGAG 
CTGCAGCAAG CTCTGTCCCA AATGGGCTAC AACCTGAGCC CCCAGTTCAC CCAGCTTCTG 
GTCTCCCGCT ACTGCCCACG CTCTGCCAAT CCTGCCATGC AGCTTGACCG CTTCATCCAG 
GTGTGCACCC AGCTGCAGGT GCTGACAGAG GCCTTCCGGG AGAAGGACAC AGCTGTACAA 
GGCAACATTC GGCTCAGCTT CGAGGACTTC GTCACCATGA CAGCTTCTCG GATGCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540047.1
CDS	16..855
Translation	MKQGCPGAAGQAPGAPPGSYYPGPPNSGGQYGSGLPPGGGYGGPAPGGPYGPPAGGGPYGHPNPGMFPSGTPGGPYGGAAPGGPYGQPPPSSYGAQQPGLYGQGGAPPNVDPEAYSWFQSVDSDHSGYISMKELKQALVNCNWSSFNDETCLMMINMFDKTKSGRIDVYGFSALWKFIQQWKNLFQQYDRDRSGSISYTELQQALSQMGYNLSPQFTQLLVSRYCPRSANPAMQLDRFIQVCTQLQVLTEAFREKDTAVQGNIRLSFEDFVTMTASRML

Target ORF information:

RefSeq Version	XM_011541745.1
Organism	Homo sapiens(human)
Definition	Homo sapiens penta-EF-hand domain containing 1 (PEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541745.1

ORF Insert Sequence:

ATGAAGCAGG GCTGCCCAGG AGCTGCAGGA CAAGCACCAG GAGCCCCTCC GGGTAGCTAC 
TACCCTGGAC CCCCCAATAG TGGAGGGCAG TATGGTAGTG GGCTACCCCC TGGTGGTGGT 
TATGGGGGTC CTGCCCCTGG AGGGCCTTAT GGACCACCAG CTGGTGGAGG GCCCTATGGA 
CACCCCAATC CTGGGATGTT CCCCTCTGGA ACTCCAGGAG GACCATATGG CGGTGCAGCT 
CCCGGGGGCC CCTATGGTCA GCCACCTCCA AGTTCCTACG GTGCCCAGCA GCCTGGGCTT 
TATGGACAGG GTGGCGCCCC TCCCAATGTG GATCCTGAGG CCTACTCCTG GTTCCAGTCG 
GTGGACTCAG ATCACAGTGG CTATATCTCC ATGAAGGAGC TAAAGCAGGC CCTGGTCAAC 
TGCAATTGGT CTTCATTCAA TGATGAGACC TGCCTCATGA TGATAAACAT GTTTGACAAG 
ACCAAGTCAG GCCGCATCGA TGTCTACGGC TTCTCAGCCC TGTGGAAATT CATCCAGCAG 
TGGAAGAACC TCTTCCAGCA GTATGACCGG GACCGCTCGG GCTCCATTAG CTACACAGAG 
CTGCAGCAAG CTCTGTCCCA AATGGGCTAC AACCTGAGCC CCCAGTTCAC CCAGCTTCTG 
GTCTCCCGCT ACTGCCCACG CTCTGCCAAT CCTGCCATGC AGCTTGACCG CTTCATCCAG 
GTGTGCACCC AGCTGCAGGT GCTGACAGAG GCCTTCCGGG AGAAGGACAC AGCTGTACAA 
GGCAACATTC GGCTCAGCTT CGAGGACTTC GTCACCATGA CAGCTTCTCG GATGCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEF1 ( XM_017001681.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857170.1 Homo sapiens penta-EF-hand domain containing 1 (PEF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu90190
Clone ID Related Accession (Same CDS sequence)	XM_017001681.1
Accession Version	XM_017001681.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	peflin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTCA CACCCCTTCC TCCAGGTGGC GCCCCTCCCA ATGTGGATCC TGAGGCCTAC 
TCCTGGTTCC AGTCGGTGGA CTCAGATCAC AGTGGCTATA TCTCCATGAA GGAGCTAAAG 
CAGGCCCTGG TCAACTGCAA TTGGTCTTCA TTCAATGATG AGACCTGCCT CATGATGATA 
AACATGTTTG ACAAGACCAA GTCAGGCCGC ATCGATGTCT ACGGCTTCTC AGCCCTGTGG 
AAATTCATCC AGCAGTGGAA GAACCTCTTC CAGCAGTATG ACCGGGACCG CTCGGGCTCC 
ATTAGCTACA CAGAGCTGCA GCAAGCTCTG TCCCAAATGG GCTACAACCT GAGCCCCCAG 
TTCACCCAGC TTCTGGTCTC CCGCTACTGC CCACGCTCTG CCAATCCTGC CATGCAGCTT 
GACCGCTTCA TCCAGGTGTG CACCCAGCTG CAGGTGCTGA CAGAGGCCTT CCGGGAGAAG 
GACACAGCTG TACAAGGCAA CATTCGGCTC AGCTTCGAGG ACTTCGTCAC CATGACAGCT 
TCTCGGATGC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857170.1
CDS	1939..2493
Translation	MAFTPLPPGGAPPNVDPEAYSWFQSVDSDHSGYISMKELKQALVNCNWSSFNDETCLMMINMFDKTKSGRIDVYGFSALWKFIQQWKNLFQQYDRDRSGSISYTELQQALSQMGYNLSPQFTQLLVSRYCPRSANPAMQLDRFIQVCTQLQVLTEAFREKDTAVQGNIRLSFEDFVTMTASRML

Target ORF information:

RefSeq Version	XM_017001681.1
Organism	Homo sapiens(human)
Definition	Homo sapiens penta-EF-hand domain containing 1 (PEF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001681.1

ORF Insert Sequence:

ATGGCCTTCA CACCCCTTCC TCCAGGTGGC GCCCCTCCCA ATGTGGATCC TGAGGCCTAC 
TCCTGGTTCC AGTCGGTGGA CTCAGATCAC AGTGGCTATA TCTCCATGAA GGAGCTAAAG 
CAGGCCCTGG TCAACTGCAA TTGGTCTTCA TTCAATGATG AGACCTGCCT CATGATGATA 
AACATGTTTG ACAAGACCAA GTCAGGCCGC ATCGATGTCT ACGGCTTCTC AGCCCTGTGG 
AAATTCATCC AGCAGTGGAA GAACCTCTTC CAGCAGTATG ACCGGGACCG CTCGGGCTCC 
ATTAGCTACA CAGAGCTGCA GCAAGCTCTG TCCCAAATGG GCTACAACCT GAGCCCCCAG 
TTCACCCAGC TTCTGGTCTC CCGCTACTGC CCACGCTCTG CCAATCCTGC CATGCAGCTT 
GACCGCTTCA TCCAGGTGTG CACCCAGCTG CAGGTGCTGA CAGAGGCCTT CCGGGAGAAG 
GACACAGCTG TACAAGGCAA CATTCGGCTC AGCTTCGAGG ACTTCGTCAC CATGACAGCT 
TCTCGGATGC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of the CUL3?Ubiquitin Ligase by a Calcium-Dependent Co-adaptor.
Cell167(2)525-538.e14(2016 Oct)
McGourty CA,Akopian D,Walsh C,Gorur A,Werner A,Schekman R,Bautista D,Rape M

Penta-EF-Hand Protein Peflin Is a Negative Regulator of ER-To-Golgi Transport.
PloS one11(6)e0157227(2016)
Rayl M,Truitt M,Held A,Sargeant J,Thorsen K,Hay JC

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Toward an understanding of the protein interaction network of the human liver.
Molecular systems biology7536(2011 Oct)
Wang J,Huo K,Ma L,Tang L,Li D,Huang X,Yuan Y,Li C,Wang W,Guan W,Chen H,Jin C,Wei J,Zhang W,Yang Y,Liu Q,Zhou Y,Zhang C,Wu Z,Xu W,Zhang Y,Liu T,Yu D,Zhang Y,Chen L,Zhu D,Zhong X,Kang L,Gan X,Yu X,Ma Q,Yan J,Zhou L,Liu Z,Zhu Y,Zhou T,He F,Yang X

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Immunology and cell biology88(8)851-6(2010 Nov-Dec)
Buschow SI,van Balkom BW,Aalberts M,Heck AJ,Wauben M,Stoorvogel W

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PEF1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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