TMEM143 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TMEM143
Entrez Gene ID	55260
Full Name	transmembrane protein 143
General protein information	Preferred Names transmembrane protein 143 Names transmembrane protein 143
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.33

mRNA and Protein(s)

mRNA	Protein	Name
NM_001303539.2	NP_001290468.1	transmembrane protein 143 isoform c
NM_018273.3	NP_060743.2	transmembrane protein 143 isoform a
NM_018273.4	NP_060743.2	transmembrane protein 143 isoform a
NM_001303538.2	NP_001290467.1	transmembrane protein 143 isoform b
NM_001303538.1	NP_001290467.1	transmembrane protein 143 isoform b
NM_001303540.1	NP_001290469.1	transmembrane protein 143 isoform d
NM_001303540.2	NP_001290469.1	transmembrane protein 143 isoform d
NM_001303539.1	NP_001290468.1	transmembrane protein 143 isoform c

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TMEM143	NP_001032524.1
Rattus norvegicus (Norway rat)	Tmem143	NP_001100983.1
Homo sapiens (human)	TMEM143	NP_060743.2
Pan troglodytes (chimpanzee)	TMEM143	XP_512792.2
Canis lupus familiaris (dog)	TMEM143	XP_854887.1
Xenopus tropicalis (tropical clawed frog)	tmem143	XP_002935033.2
Mus musculus (house mouse)	Tmem143	NP_659050.2

Gene Ontology
Bibliography

Related articles in PubMed

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Bailey SD, Xie C, Do R, Montpetit A, Diaz R, Mohan V, Keavney B, Yusuf S, Gerstein HC, Engert JC, Anand S,
Diabetes care33(10)2250-3(2010 Oct)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.
Talmud PJ, Drenos F, Shah S, Shah T, Palmen J, Verzilli C, Gaunt TR, Pallas J, Lovering R, Li K, Casas JP, Sofat R, Kumari M, Rodriguez S, Johnson T, Newhouse SJ, Dominiczak A, Samani NJ, Caulfield M, Sever P, Stanton A, Shields DC, Padmanabhan S, Melander O, Hastie C, Delles C, Ebrahim S, Marmot MG, Smith GD, Lawlor DA, Munroe PB, Day IN, Kivimaki M, Whittaker J, Humphries SE, Hingorani AD, , ,
American journal of human genetics85(5)628-42(2009 Nov)

The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.
Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C, Currell B, Deuel B, Dowd P, Eaton D, Foster J, Grimaldi C, Gu Q, Hass PE, Heldens S, Huang A, Kim HS, Klimowski L, Jin Y, Johnson S, Lee J, Lewis L, Liao D, Mark M, Robbie E, Sanchez C, Schoenfeld J, Seshagiri S, Simmons L, Singh J, Smith V, Stinson J, Vagts A, Vandlen R, Watanabe C, Wieand D, Woods K, Xie MH, Yansura D, Yi S, Yu G, Yuan J, Zhang M, Zhang Z, Goddard A, Wood WI, Godowski P, Gray A
Genome research13(10)2265-70(2003 Oct)

Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing.
Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S
EMBO reports1(3)287-92(2000 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

The following TMEM143 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEM143 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu69246	NM_018273.4 Latest version!	Homo sapiens transmembrane protein 143 (TMEM143), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$272.30 $389.00
OHu69247	NM_001303539.2 Latest version!	Homo sapiens transmembrane protein 143 (TMEM143), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu69248	NM_001303538.2 Latest version!	Homo sapiens transmembrane protein 143 (TMEM143), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu69249	NM_001303540.2 Latest version!	Homo sapiens transmembrane protein 143 (TMEM143), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu69246	NM_018273.3	Homo sapiens transmembrane protein 143 (TMEM143), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$272.30 $389.00
OHu69248	NM_001303538.1	Homo sapiens transmembrane protein 143 (TMEM143), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu69249	NM_001303540.1	Homo sapiens transmembrane protein 143 (TMEM143), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu69247	NM_001303539.1	Homo sapiens transmembrane protein 143 (TMEM143), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMEM143 ( NM_018273.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060743.2 Homo sapiens transmembrane protein 143 (TMEM143), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu69246
Clone ID Related Accession (Same CDS sequence)	NM_018273.3 , NM_018273.4
Accession Version	NM_018273.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	transmembrane protein 143 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC357566.1, AC011514.3, BC016919.1, AC008392.6 and AW136519.1. On Jan 13, 2015 this sequence version replaced NM_018273.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC040021.1, SRR1660803.147271.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153932, SAMEA2154405 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAGTCG AGCTTTGGCT AAGGCTCCGG GGAAAGGGTC TAGCCATGCT GCATGTGACC 
CGGGGGGTCT GGGGGTCCAG GGTCCGAGTA TGGCCACTGT TGCCCGCGCT CCTCGGGCCC 
CCCCGGGCCC TCTCATCGCT GGCAGCCAAA ATGGGGGAGT ATCGCAAGAT GTGGAACCCC 
AGGGAGCCCC GCGACTGGGC CCAGCAGTAC CGCGAGCGCT TCATTCCCTT CTCCAAGGAG 
CAGCTGCTCC GCCTCCTAAT ACAGGAATTC CACTCGAGTC CGGCAGAGAA GGCGGCTTTG 
GAGGCGTTCT CGGCCCACGT GGACTTCTGC ACCCTGTTCC ACTACCACCA AATCCTGGCC 
CGGCTGCAGG CCTTATATGA CCCCATCAAC CCTGACAGGG AGACCCTCGA TCAGCCATCA 
CTAACGGATC CCCAGCGTCT GTCTAATGAG CAGGAGGTGC TTCGGGCTCT GGAGCCCCTG 
CTGGCCCAGG CCAACTTCTC CCCGCTGTCT GAGGACACCC TGGCCTACGC GCTGGTGGTC 
CACCACCCTC AGGATGAGGT CCAGGTGACA GTAAATTTGG ATCAGTATGT CTACATTCAC 
TTCTGGGCCC TGGGCCAGCG AGTCGGGCAG ATGCCCCTGA AGTCCAGCGT GGGCTCCAGG 
CGTGGCTTCT TCACCAAGCT GCCCCCTGCG GAGAGGAGAT ACTTTAAGCG GGTGGTCCTG 
GCAGCCCGGA CCAAACGGGG ACACCTGGTA CTGAAGAGTT TCAAGGACAC GCCGCTGGAA 
GGCCTGGAGC AGCTGCTGCC GGAGCTGAAG GTGCGCACGC CCACCCTGCA GCGCGCCCTG 
CTCAACCTCA TGCTGGTAGT CTCCGGCGTG GCGATCTTCG TCAACGTGGG CATGGTGGTG 
CTAACCGACC TCAAGGTGGC CACCTCCCTG CTGCTGCTGC TCTTCGCCAT CTTCATGGGC 
CTGCGGGCCT CCAAGATGTT CGGGCAGCGG CGCAGCGCGC AGGCGTTGGA GCTGGCGCAC 
ATGCTGTACT ATCGCAGTAC GTCCAACAAC TCGGAGCTGC TCAGCGCCCT GGCCCTGCGC 
GCGCAGGACG AGCACACCAA GGAGGCGCTG CTGGCTCACA GCTTCCTGGC CCGGCGGCCA 
GGGGGCACTC AAGGCTCGCC CGAAGAGACC TCCAGGTGGC TCCGGTCGGA GGTGGAGAAC 
TGGCTCCTAG CCAAGTCAGG CTGTGAGGTG ACCTTCAACG GAACTCGGGC CCTGGCGCAT 
CTGCAGGCCC TGACCCCCAG CATGGGATTG TACCCACCCC CGGGTTTCCC CAAACTAGAC 
CCAGTGGCTC CGATCACTTC CGAGCCCCCG CAAGCCACGC CCAGCAGTAA CATCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060743.2
CDS	318..1697
Translation	MTVELWLRLRGKGLAMLHVTRGVWGSRVRVWPLLPALLGPPRALSSLAAKMGEYRKMWNPREPRDWAQQYRERFIPFSKEQLLRLLIQEFHSSPAEKAALEAFSAHVDFCTLFHYHQILARLQALYDPINPDRETLDQPSLTDPQRLSNEQEVLRALEPLLAQANFSPLSEDTLAYALVVHHPQDEVQVTVNLDQYVYIHFWALGQRVGQMPLKSSVGSRRGFFTKLPPAERRYFKRVVLAARTKRGHLVLKSFKDTPLEGLEQLLPELKVRTPTLQRALLNLMLVVSGVAIFVNVGMVVLTDLKVATSLLLLLFAIFMGLRASKMFGQRRSAQALELAHMLYYRSTSNNSELLSALALRAQDEHTKEALLAHSFLARRPGGTQGSPEETSRWLRSEVENWLLAKSGCEVTFNGTRALAHLQALTPSMGLYPPPGFPKLDPVAPITSEPPQATPSSNIS

Target ORF information:

RefSeq Version	NM_018273.3
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 143 (TMEM143), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018273.3

ORF Insert Sequence:

ATGACAGTCG AGCTTTGGCT AAGGCTCCGG GGAAAGGGTC TAGCCATGCT GCATGTGACC 
CGGGGGGTCT GGGGGTCCAG GGTCCGAGTA TGGCCACTGT TGCCCGCGCT CCTCGGGCCC 
CCCCGGGCCC TCTCATCGCT GGCAGCCAAA ATGGGGGAGT ATCGCAAGAT GTGGAACCCC 
AGGGAGCCCC GCGACTGGGC CCAGCAGTAC CGCGAGCGCT TCATTCCCTT CTCCAAGGAG 
CAGCTGCTCC GCCTCCTAAT ACAGGAATTC CACTCGAGTC CGGCAGAGAA GGCGGCTTTG 
GAGGCGTTCT CGGCCCACGT GGACTTCTGC ACCCTGTTCC ACTACCACCA AATCCTGGCC 
CGGCTGCAGG CCTTATATGA CCCCATCAAC CCTGACAGGG AGACCCTCGA TCAGCCATCA 
CTAACGGATC CCCAGCGTCT GTCTAATGAG CAGGAGGTGC TTCGGGCTCT GGAGCCCCTG 
CTGGCCCAGG CCAACTTCTC CCCGCTGTCT GAGGACACCC TGGCCTACGC GCTGGTGGTC 
CACCACCCTC AGGATGAGGT CCAGGTGACA GTAAATTTGG ATCAGTATGT CTACATTCAC 
TTCTGGGCCC TGGGCCAGCG AGTCGGGCAG ATGCCCCTGA AGTCCAGCGT GGGCTCCAGG 
CGTGGCTTCT TCACCAAGCT GCCCCCTGCG GAGAGGAGAT ACTTTAAGCG GGTGGTCCTG 
GCAGCCCGGA CCAAACGGGG ACACCTGGTA CTGAAGAGTT TCAAGGACAC GCCGCTGGAA 
GGCCTGGAGC AGCTGCTGCC GGAGCTGAAG GTGCGCACGC CCACCCTGCA GCGCGCCCTG 
CTCAACCTCA TGCTGGTAGT CTCCGGCGTG GCGATCTTCG TCAACGTGGG CATGGTGGTG 
CTAACCGACC TCAAGGTGGC CACCTCCCTG CTGCTGCTGC TCTTCGCCAT CTTCATGGGC 
CTGCGGGCCT CCAAGATGTT CGGGCAGCGG CGCAGCGCGC AGGCGTTGGA GCTGGCGCAC 
ATGCTGTACT ATCGCAGTAC GTCCAACAAC TCGGAGCTGC TCAGCGCCCT GGCCCTGCGC 
GCGCAGGACG AGCACACCAA GGAGGCGCTG CTGGCTCACA GCTTCCTGGC CCGGCGGCCA 
GGGGGCACTC AAGGCTCGCC CGAAGAGACC TCCAGGTGGC TCCGGTCGGA GGTGGAGAAC 
TGGCTCCTAG CCAAGTCAGG CTGTGAGGTG ACCTTCAACG GAACTCGGGC CCTGGCGCAT 
CTGCAGGCCC TGACCCCCAG CATGGGATTG TACCCACCCC CGGGTTTCCC CAAACTAGAC 
CCAGTGGCTC CGATCACTTC CGAGCCCCCG CAAGCCACGC CCAGCAGTAA CATCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM143 ( NM_018273.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060743.2 Homo sapiens transmembrane protein 143 (TMEM143), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu69246
Clone ID Related Accession (Same CDS sequence)	NM_018273.3 , NM_018273.4
Accession Version	NM_018273.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	transmembrane protein 143 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC357566.1, BC016919.1, AC008392.6 and AW136519.1. On Nov 22, 2018 this sequence version replaced NM_018273.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK129801.1, SRR1803616.113790.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153932, SAMEA2154405 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000293261.8/ ENSP00000293261.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACAGTCG AGCTTTGGCT AAGGCTCCGG GGAAAGGGTC TAGCCATGCT GCATGTGACC 
CGGGGGGTCT GGGGGTCCAG GGTCCGAGTA TGGCCACTGT TGCCCGCGCT CCTCGGGCCC 
CCCCGGGCCC TCTCATCGCT GGCAGCCAAA ATGGGGGAGT ATCGCAAGAT GTGGAACCCC 
AGGGAGCCCC GCGACTGGGC CCAGCAGTAC CGCGAGCGCT TCATTCCCTT CTCCAAGGAG 
CAGCTGCTCC GCCTCCTAAT ACAGGAATTC CACTCGAGTC CGGCAGAGAA GGCGGCTTTG 
GAGGCGTTCT CGGCCCACGT GGACTTCTGC ACCCTGTTCC ACTACCACCA AATCCTGGCC 
CGGCTGCAGG CCTTATATGA CCCCATCAAC CCTGACAGGG AGACCCTCGA TCAGCCATCA 
CTAACGGATC CCCAGCGTCT GTCTAATGAG CAGGAGGTGC TTCGGGCTCT GGAGCCCCTG 
CTGGCCCAGG CCAACTTCTC CCCGCTGTCT GAGGACACCC TGGCCTACGC GCTGGTGGTC 
CACCACCCTC AGGATGAGGT CCAGGTGACA GTAAATTTGG ATCAGTATGT CTACATTCAC 
TTCTGGGCCC TGGGCCAGCG AGTCGGGCAG ATGCCCCTGA AGTCCAGCGT GGGCTCCAGG 
CGTGGCTTCT TCACCAAGCT GCCCCCTGCG GAGAGGAGAT ACTTTAAGCG GGTGGTCCTG 
GCAGCCCGGA CCAAACGGGG ACACCTGGTA CTGAAGAGTT TCAAGGACAC GCCGCTGGAA 
GGCCTGGAGC AGCTGCTGCC GGAGCTGAAG GTGCGCACGC CCACCCTGCA GCGCGCCCTG 
CTCAACCTCA TGCTGGTAGT CTCCGGCGTG GCGATCTTCG TCAACGTGGG CATGGTGGTG 
CTAACCGACC TCAAGGTGGC CACCTCCCTG CTGCTGCTGC TCTTCGCCAT CTTCATGGGC 
CTGCGGGCCT CCAAGATGTT CGGGCAGCGG CGCAGCGCGC AGGCGTTGGA GCTGGCGCAC 
ATGCTGTACT ATCGCAGTAC GTCCAACAAC TCGGAGCTGC TCAGCGCCCT GGCCCTGCGC 
GCGCAGGACG AGCACACCAA GGAGGCGCTG CTGGCTCACA GCTTCCTGGC CCGGCGGCCA 
GGGGGCACTC AAGGCTCGCC CGAAGAGACC TCCAGGTGGC TCCGGTCGGA GGTGGAGAAC 
TGGCTCCTAG CCAAGTCAGG CTGTGAGGTG ACCTTCAACG GAACTCGGGC CCTGGCGCAT 
CTGCAGGCCC TGACCCCCAG CATGGGATTG TACCCACCCC CGGGTTTCCC CAAACTAGAC 
CCAGTGGCTC CGATCACTTC CGAGCCCCCG CAAGCCACGC CCAGCAGTAA CATCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060743.2
CDS	21..1400
Translation	MTVELWLRLRGKGLAMLHVTRGVWGSRVRVWPLLPALLGPPRALSSLAAKMGEYRKMWNPREPRDWAQQYRERFIPFSKEQLLRLLIQEFHSSPAEKAALEAFSAHVDFCTLFHYHQILARLQALYDPINPDRETLDQPSLTDPQRLSNEQEVLRALEPLLAQANFSPLSEDTLAYALVVHHPQDEVQVTVNLDQYVYIHFWALGQRVGQMPLKSSVGSRRGFFTKLPPAERRYFKRVVLAARTKRGHLVLKSFKDTPLEGLEQLLPELKVRTPTLQRALLNLMLVVSGVAIFVNVGMVVLTDLKVATSLLLLLFAIFMGLRASKMFGQRRSAQALELAHMLYYRSTSNNSELLSALALRAQDEHTKEALLAHSFLARRPGGTQGSPEETSRWLRSEVENWLLAKSGCEVTFNGTRALAHLQALTPSMGLYPPPGFPKLDPVAPITSEPPQATPSSNIS

Target ORF information:

RefSeq Version	NM_018273.4
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 143 (TMEM143), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018273.4

ORF Insert Sequence:

ATGACAGTCG AGCTTTGGCT AAGGCTCCGG GGAAAGGGTC TAGCCATGCT GCATGTGACC 
CGGGGGGTCT GGGGGTCCAG GGTCCGAGTA TGGCCACTGT TGCCCGCGCT CCTCGGGCCC 
CCCCGGGCCC TCTCATCGCT GGCAGCCAAA ATGGGGGAGT ATCGCAAGAT GTGGAACCCC 
AGGGAGCCCC GCGACTGGGC CCAGCAGTAC CGCGAGCGCT TCATTCCCTT CTCCAAGGAG 
CAGCTGCTCC GCCTCCTAAT ACAGGAATTC CACTCGAGTC CGGCAGAGAA GGCGGCTTTG 
GAGGCGTTCT CGGCCCACGT GGACTTCTGC ACCCTGTTCC ACTACCACCA AATCCTGGCC 
CGGCTGCAGG CCTTATATGA CCCCATCAAC CCTGACAGGG AGACCCTCGA TCAGCCATCA 
CTAACGGATC CCCAGCGTCT GTCTAATGAG CAGGAGGTGC TTCGGGCTCT GGAGCCCCTG 
CTGGCCCAGG CCAACTTCTC CCCGCTGTCT GAGGACACCC TGGCCTACGC GCTGGTGGTC 
CACCACCCTC AGGATGAGGT CCAGGTGACA GTAAATTTGG ATCAGTATGT CTACATTCAC 
TTCTGGGCCC TGGGCCAGCG AGTCGGGCAG ATGCCCCTGA AGTCCAGCGT GGGCTCCAGG 
CGTGGCTTCT TCACCAAGCT GCCCCCTGCG GAGAGGAGAT ACTTTAAGCG GGTGGTCCTG 
GCAGCCCGGA CCAAACGGGG ACACCTGGTA CTGAAGAGTT TCAAGGACAC GCCGCTGGAA 
GGCCTGGAGC AGCTGCTGCC GGAGCTGAAG GTGCGCACGC CCACCCTGCA GCGCGCCCTG 
CTCAACCTCA TGCTGGTAGT CTCCGGCGTG GCGATCTTCG TCAACGTGGG CATGGTGGTG 
CTAACCGACC TCAAGGTGGC CACCTCCCTG CTGCTGCTGC TCTTCGCCAT CTTCATGGGC 
CTGCGGGCCT CCAAGATGTT CGGGCAGCGG CGCAGCGCGC AGGCGTTGGA GCTGGCGCAC 
ATGCTGTACT ATCGCAGTAC GTCCAACAAC TCGGAGCTGC TCAGCGCCCT GGCCCTGCGC 
GCGCAGGACG AGCACACCAA GGAGGCGCTG CTGGCTCACA GCTTCCTGGC CCGGCGGCCA 
GGGGGCACTC AAGGCTCGCC CGAAGAGACC TCCAGGTGGC TCCGGTCGGA GGTGGAGAAC 
TGGCTCCTAG CCAAGTCAGG CTGTGAGGTG ACCTTCAACG GAACTCGGGC CCTGGCGCAT 
CTGCAGGCCC TGACCCCCAG CATGGGATTG TACCCACCCC CGGGTTTCCC CAAACTAGAC 
CCAGTGGCTC CGATCACTTC CGAGCCCCCG CAAGCCACGC CCAGCAGTAA CATCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM143 ( NM_001303539.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001290468.1 Homo sapiens transmembrane protein 143 (TMEM143), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu69247
Clone ID Related Accession (Same CDS sequence)	NM_001303539.2 , NM_001303539.1
Accession Version	NM_001303539.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	transmembrane protein 143 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC357566.1, AK298317.1, AC008392.6 and AW136519.1. On May 31, 2019 this sequence version replaced NM_001303539.1. Transcript Variant: This variant (3) lacks an in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (c) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.212922.1, AK298317.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAGTCG AGCTTTGGCT AAGGCTCCGG GGAAAGGGTC TAGCCATGCT GCATGTGACC 
CGGGGGGTCT GGGGGTCCAG GGTCCGAGTA TGGCCACTGT TGCCCGCGCT CCTCGGGCCC 
CCCCGGGCCC TCTCATCGCT GGCAGCCAAA ATGGGGGAGT ATCGCAAGAT GTGGAACCCC 
AGGGAGCCCC GCGACTGGGC CCAGCAGTAC CGCGAGCGCT TCATTCCCTT CTCCAAGGAG 
CAGCTGCTCC GCCTCCTAAT ACAGGAATTC CACTCGAGTC CGGCAGAGAA GGCGGCTTTG 
GAGGCGTTCT CGGCCCACGT GGACTTCTGC ACCCTGTTCC ACTACCACCA AATCCTGGCC 
CGGCTGCAGG TGACAGTAAA TTTGGATCAG TATGTCTACA TTCACTTCTG GGCCCTGGGC 
CAGCGAGTCG GGCAGATGCC CCTGAAGTCC AGCGTGGGCT CCAGGCGTGG CTTCTTCACC 
AAGCTGCCCC CTGCGGAGAG GAGATACTTT AAGCGGGTGG TCCTGGCAGC CCGGACCAAA 
CGGGGACACC TGGTACTGAA GAGTTTCAAG GACACGCCGC TGGAAGGCCT GGAGCAGCTG 
CTGCCGGAGC TGAAGGTGCG CACGCCCACC CTGCAGCGCG CCCTGCTCAA CCTCATGCTG 
GTAGTCTCCG GCGTGGCGAT CTTCGTCAAC GTGGGCATGG TGGTGCTAAC CGACCTCAAG 
GTGGCCACCT CCCTGCTGCT GCTGCTCTTC GCCATCTTCA TGGGCCTGCG GGCCTCCAAG 
ATGTTCGGGC AGCGGCGCAG CGCGCAGGCG TTGGAGCTGG CGCACATGCT GTACTATCGC 
AGTACGTCCA ACAACTCGGA GCTGCTCAGC GCCCTGGCCC TGCGCGCGCA GGACGAGCAC 
ACCAAGGAGG CGCTGCTGGC TCACAGCTTC CTGGCCCGGC GGCCAGGGGG CACTCAAGGC 
TCGCCCGAAG AGACCTCCAG GTGGCTCCGG TCGGAGGTGG AGAACTGGCT CCTAGCCAAG 
TCAGGCTGTG AGGTGACCTT CAACGGAACT CGGGCCCTGG CGCATCTGCA GGCCCTGACC 
CCCAGCATGG GATTGTACCC ACCCCCGGGT TTCCCCAAAC TAGACCCAGT GGCTCCGATC 
ACTTCCGAGC CCCCGCAAGC CACGCCCAGC AGTAACATCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001290468.1
CDS	21..1205
Translation	MTVELWLRLRGKGLAMLHVTRGVWGSRVRVWPLLPALLGPPRALSSLAAKMGEYRKMWNPREPRDWAQQYRERFIPFSKEQLLRLLIQEFHSSPAEKAALEAFSAHVDFCTLFHYHQILARLQVTVNLDQYVYIHFWALGQRVGQMPLKSSVGSRRGFFTKLPPAERRYFKRVVLAARTKRGHLVLKSFKDTPLEGLEQLLPELKVRTPTLQRALLNLMLVVSGVAIFVNVGMVVLTDLKVATSLLLLLFAIFMGLRASKMFGQRRSAQALELAHMLYYRSTSNNSELLSALALRAQDEHTKEALLAHSFLARRPGGTQGSPEETSRWLRSEVENWLLAKSGCEVTFNGTRALAHLQALTPSMGLYPPPGFPKLDPVAPITSEPPQATPSSNIS

Target ORF information:

RefSeq Version	NM_001303539.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 143 (TMEM143), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001303539.2

ORF Insert Sequence:

ATGACAGTCG AGCTTTGGCT AAGGCTCCGG GGAAAGGGTC TAGCCATGCT GCATGTGACC 
CGGGGGGTCT GGGGGTCCAG GGTCCGAGTA TGGCCACTGT TGCCCGCGCT CCTCGGGCCC 
CCCCGGGCCC TCTCATCGCT GGCAGCCAAA ATGGGGGAGT ATCGCAAGAT GTGGAACCCC 
AGGGAGCCCC GCGACTGGGC CCAGCAGTAC CGCGAGCGCT TCATTCCCTT CTCCAAGGAG 
CAGCTGCTCC GCCTCCTAAT ACAGGAATTC CACTCGAGTC CGGCAGAGAA GGCGGCTTTG 
GAGGCGTTCT CGGCCCACGT GGACTTCTGC ACCCTGTTCC ACTACCACCA AATCCTGGCC 
CGGCTGCAGG TGACAGTAAA TTTGGATCAG TATGTCTACA TTCACTTCTG GGCCCTGGGC 
CAGCGAGTCG GGCAGATGCC CCTGAAGTCC AGCGTGGGCT CCAGGCGTGG CTTCTTCACC 
AAGCTGCCCC CTGCGGAGAG GAGATACTTT AAGCGGGTGG TCCTGGCAGC CCGGACCAAA 
CGGGGACACC TGGTACTGAA GAGTTTCAAG GACACGCCGC TGGAAGGCCT GGAGCAGCTG 
CTGCCGGAGC TGAAGGTGCG CACGCCCACC CTGCAGCGCG CCCTGCTCAA CCTCATGCTG 
GTAGTCTCCG GCGTGGCGAT CTTCGTCAAC GTGGGCATGG TGGTGCTAAC CGACCTCAAG 
GTGGCCACCT CCCTGCTGCT GCTGCTCTTC GCCATCTTCA TGGGCCTGCG GGCCTCCAAG 
ATGTTCGGGC AGCGGCGCAG CGCGCAGGCG TTGGAGCTGG CGCACATGCT GTACTATCGC 
AGTACGTCCA ACAACTCGGA GCTGCTCAGC GCCCTGGCCC TGCGCGCGCA GGACGAGCAC 
ACCAAGGAGG CGCTGCTGGC TCACAGCTTC CTGGCCCGGC GGCCAGGGGG CACTCAAGGC 
TCGCCCGAAG AGACCTCCAG GTGGCTCCGG TCGGAGGTGG AGAACTGGCT CCTAGCCAAG 
TCAGGCTGTG AGGTGACCTT CAACGGAACT CGGGCCCTGG CGCATCTGCA GGCCCTGACC 
CCCAGCATGG GATTGTACCC ACCCCCGGGT TTCCCCAAAC TAGACCCAGT GGCTCCGATC 
ACTTCCGAGC CCCCGCAAGC CACGCCCAGC AGTAACATCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM143 ( NM_001303539.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001290468.1 Homo sapiens transmembrane protein 143 (TMEM143), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu69247
Clone ID Related Accession (Same CDS sequence)	NM_001303539.2 , NM_001303539.1
Accession Version	NM_001303539.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	transmembrane protein 143 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC357566.1, AC011514.3, AK298317.1, AC008392.6 and AW136519.1. Transcript Variant: This variant (3) lacks an in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (c) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.212922.1, AK298317.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAGTCG AGCTTTGGCT AAGGCTCCGG GGAAAGGGTC TAGCCATGCT GCATGTGACC 
CGGGGGGTCT GGGGGTCCAG GGTCCGAGTA TGGCCACTGT TGCCCGCGCT CCTCGGGCCC 
CCCCGGGCCC TCTCATCGCT GGCAGCCAAA ATGGGGGAGT ATCGCAAGAT GTGGAACCCC 
AGGGAGCCCC GCGACTGGGC CCAGCAGTAC CGCGAGCGCT TCATTCCCTT CTCCAAGGAG 
CAGCTGCTCC GCCTCCTAAT ACAGGAATTC CACTCGAGTC CGGCAGAGAA GGCGGCTTTG 
GAGGCGTTCT CGGCCCACGT GGACTTCTGC ACCCTGTTCC ACTACCACCA AATCCTGGCC 
CGGCTGCAGG TGACAGTAAA TTTGGATCAG TATGTCTACA TTCACTTCTG GGCCCTGGGC 
CAGCGAGTCG GGCAGATGCC CCTGAAGTCC AGCGTGGGCT CCAGGCGTGG CTTCTTCACC 
AAGCTGCCCC CTGCGGAGAG GAGATACTTT AAGCGGGTGG TCCTGGCAGC CCGGACCAAA 
CGGGGACACC TGGTACTGAA GAGTTTCAAG GACACGCCGC TGGAAGGCCT GGAGCAGCTG 
CTGCCGGAGC TGAAGGTGCG CACGCCCACC CTGCAGCGCG CCCTGCTCAA CCTCATGCTG 
GTAGTCTCCG GCGTGGCGAT CTTCGTCAAC GTGGGCATGG TGGTGCTAAC CGACCTCAAG 
GTGGCCACCT CCCTGCTGCT GCTGCTCTTC GCCATCTTCA TGGGCCTGCG GGCCTCCAAG 
ATGTTCGGGC AGCGGCGCAG CGCGCAGGCG TTGGAGCTGG CGCACATGCT GTACTATCGC 
AGTACGTCCA ACAACTCGGA GCTGCTCAGC GCCCTGGCCC TGCGCGCGCA GGACGAGCAC 
ACCAAGGAGG CGCTGCTGGC TCACAGCTTC CTGGCCCGGC GGCCAGGGGG CACTCAAGGC 
TCGCCCGAAG AGACCTCCAG GTGGCTCCGG TCGGAGGTGG AGAACTGGCT CCTAGCCAAG 
TCAGGCTGTG AGGTGACCTT CAACGGAACT CGGGCCCTGG CGCATCTGCA GGCCCTGACC 
CCCAGCATGG GATTGTACCC ACCCCCGGGT TTCCCCAAAC TAGACCCAGT GGCTCCGATC 
ACTTCCGAGC CCCCGCAAGC CACGCCCAGC AGTAACATCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001290468.1
CDS	318..1502
Translation	MTVELWLRLRGKGLAMLHVTRGVWGSRVRVWPLLPALLGPPRALSSLAAKMGEYRKMWNPREPRDWAQQYRERFIPFSKEQLLRLLIQEFHSSPAEKAALEAFSAHVDFCTLFHYHQILARLQVTVNLDQYVYIHFWALGQRVGQMPLKSSVGSRRGFFTKLPPAERRYFKRVVLAARTKRGHLVLKSFKDTPLEGLEQLLPELKVRTPTLQRALLNLMLVVSGVAIFVNVGMVVLTDLKVATSLLLLLFAIFMGLRASKMFGQRRSAQALELAHMLYYRSTSNNSELLSALALRAQDEHTKEALLAHSFLARRPGGTQGSPEETSRWLRSEVENWLLAKSGCEVTFNGTRALAHLQALTPSMGLYPPPGFPKLDPVAPITSEPPQATPSSNIS

Target ORF information:

RefSeq Version	NM_001303539.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 143 (TMEM143), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001303539.1

ORF Insert Sequence:

ATGACAGTCG AGCTTTGGCT AAGGCTCCGG GGAAAGGGTC TAGCCATGCT GCATGTGACC 
CGGGGGGTCT GGGGGTCCAG GGTCCGAGTA TGGCCACTGT TGCCCGCGCT CCTCGGGCCC 
CCCCGGGCCC TCTCATCGCT GGCAGCCAAA ATGGGGGAGT ATCGCAAGAT GTGGAACCCC 
AGGGAGCCCC GCGACTGGGC CCAGCAGTAC CGCGAGCGCT TCATTCCCTT CTCCAAGGAG 
CAGCTGCTCC GCCTCCTAAT ACAGGAATTC CACTCGAGTC CGGCAGAGAA GGCGGCTTTG 
GAGGCGTTCT CGGCCCACGT GGACTTCTGC ACCCTGTTCC ACTACCACCA AATCCTGGCC 
CGGCTGCAGG TGACAGTAAA TTTGGATCAG TATGTCTACA TTCACTTCTG GGCCCTGGGC 
CAGCGAGTCG GGCAGATGCC CCTGAAGTCC AGCGTGGGCT CCAGGCGTGG CTTCTTCACC 
AAGCTGCCCC CTGCGGAGAG GAGATACTTT AAGCGGGTGG TCCTGGCAGC CCGGACCAAA 
CGGGGACACC TGGTACTGAA GAGTTTCAAG GACACGCCGC TGGAAGGCCT GGAGCAGCTG 
CTGCCGGAGC TGAAGGTGCG CACGCCCACC CTGCAGCGCG CCCTGCTCAA CCTCATGCTG 
GTAGTCTCCG GCGTGGCGAT CTTCGTCAAC GTGGGCATGG TGGTGCTAAC CGACCTCAAG 
GTGGCCACCT CCCTGCTGCT GCTGCTCTTC GCCATCTTCA TGGGCCTGCG GGCCTCCAAG 
ATGTTCGGGC AGCGGCGCAG CGCGCAGGCG TTGGAGCTGG CGCACATGCT GTACTATCGC 
AGTACGTCCA ACAACTCGGA GCTGCTCAGC GCCCTGGCCC TGCGCGCGCA GGACGAGCAC 
ACCAAGGAGG CGCTGCTGGC TCACAGCTTC CTGGCCCGGC GGCCAGGGGG CACTCAAGGC 
TCGCCCGAAG AGACCTCCAG GTGGCTCCGG TCGGAGGTGG AGAACTGGCT CCTAGCCAAG 
TCAGGCTGTG AGGTGACCTT CAACGGAACT CGGGCCCTGG CGCATCTGCA GGCCCTGACC 
CCCAGCATGG GATTGTACCC ACCCCCGGGT TTCCCCAAAC TAGACCCAGT GGCTCCGATC 
ACTTCCGAGC CCCCGCAAGC CACGCCCAGC AGTAACATCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM143 ( NM_001303538.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001290467.1 Homo sapiens transmembrane protein 143 (TMEM143), transcript variant 2, mRNA.

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CloneID	OHu69248
Clone ID Related Accession (Same CDS sequence)	NM_001303538.2 , NM_001303538.1
Accession Version	NM_001303538.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	transmembrane protein 143 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC357566.1, AK297610.1, AC008392.6 and AW136519.1. On May 31, 2019 this sequence version replaced NM_001303538.1. Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (b) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.251435.1, AK297610.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153932, SAMEA2154405 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAGTCG AGCTTTGGCT AAGGCTCCGG GGAAAGGGTC TAGCCATGCT GCATGTGACC 
CGGGGGGTCT GGGGGTCCAG GGTCCGAGTA TGGCCACTGT TGCCCGCGCT CCTCGGGCCC 
CCCCGGGCCC TCTCATCGCT GGCAGCCAAA ATGGGGGAGT ATCGCAAGAT GTGGAACCCC 
AGGGAGCCCC GCGACTGGGC CCAGCAGTAC CGCGAGCGCT TCATTCCCTT CTCCAAGGAG 
CAGCTGCTCC GCCTCCTAAT ACAGGCCTTA TATGACCCCA TCAACCCTGA CAGGGAGACC 
CTCGATCAGC CATCACTAAC GGATCCCCAG CGTCTGTCTA ATGAGCAGGA GGTGCTTCGG 
GCTCTGGAGC CCCTGCTGGC CCAGGCCAAC TTCTCCCCGC TGTCTGAGGA CACCCTGGCC 
TACGCGCTGG TGGTCCACCA CCCTCAGGAT GAGGTCCAGG TGACAGTAAA TTTGGATCAG 
TATGTCTACA TTCACTTCTG GGCCCTGGGC CAGCGAGTCG GGCAGATGCC CCTGAAGTCC 
AGCGTGGGCT CCAGGCGTGG CTTCTTCACC AAGCTGCCCC CTGCGGAGAG GAGATACTTT 
AAGCGGGTGG TCCTGGCAGC CCGGACCAAA CGGGGACACC TGGTACTGAA GAGTTTCAAG 
GACACGCCGC TGGAAGGCCT GGAGCAGCTG CTGCCGGAGC TGAAGGTGCG CACGCCCACC 
CTGCAGCGCG CCCTGCTCAA CCTCATGCTG GTAGTCTCCG GCGTGGCGAT CTTCGTCAAC 
GTGGGCATGG TGGTGCTAAC CGACCTCAAG GTGGCCACCT CCCTGCTGCT GCTGCTCTTC 
GCCATCTTCA TGGGCCTGCG GGCCTCCAAG ATGTTCGGGC AGCGGCGCAG CGCGCAGGCG 
TTGGAGCTGG CGCACATGCT GTACTATCGC AGTACGTCCA ACAACTCGGA GCTGCTCAGC 
GCCCTGGCCC TGCGCGCGCA GGACGAGCAC ACCAAGGAGG CGCTGCTGGC TCACAGCTTC 
CTGGCCCGGC GGCCAGGGGG CACTCAAGGC TCGCCCGAAG AGACCTCCAG GTGGCTCCGG 
TCGGAGGTGG AGAACTGGCT CCTAGCCAAG TCAGGCTGTG AGGTGACCTT CAACGGAACT 
CGGGCCCTGG CGCATCTGCA GGCCCTGACC CCCAGCATGG GATTGTACCC ACCCCCGGGT 
TTCCCCAAAC TAGACCCAGT GGCTCCGATC ACTTCCGAGC CCCCGCAAGC CACGCCCAGC 
AGTAACATCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001290467.1
CDS	21..1295
Translation	MTVELWLRLRGKGLAMLHVTRGVWGSRVRVWPLLPALLGPPRALSSLAAKMGEYRKMWNPREPRDWAQQYRERFIPFSKEQLLRLLIQALYDPINPDRETLDQPSLTDPQRLSNEQEVLRALEPLLAQANFSPLSEDTLAYALVVHHPQDEVQVTVNLDQYVYIHFWALGQRVGQMPLKSSVGSRRGFFTKLPPAERRYFKRVVLAARTKRGHLVLKSFKDTPLEGLEQLLPELKVRTPTLQRALLNLMLVVSGVAIFVNVGMVVLTDLKVATSLLLLLFAIFMGLRASKMFGQRRSAQALELAHMLYYRSTSNNSELLSALALRAQDEHTKEALLAHSFLARRPGGTQGSPEETSRWLRSEVENWLLAKSGCEVTFNGTRALAHLQALTPSMGLYPPPGFPKLDPVAPITSEPPQATPSSNIS

Target ORF information:

RefSeq Version	NM_001303538.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 143 (TMEM143), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001303538.2

ORF Insert Sequence:

ATGACAGTCG AGCTTTGGCT AAGGCTCCGG GGAAAGGGTC TAGCCATGCT GCATGTGACC 
CGGGGGGTCT GGGGGTCCAG GGTCCGAGTA TGGCCACTGT TGCCCGCGCT CCTCGGGCCC 
CCCCGGGCCC TCTCATCGCT GGCAGCCAAA ATGGGGGAGT ATCGCAAGAT GTGGAACCCC 
AGGGAGCCCC GCGACTGGGC CCAGCAGTAC CGCGAGCGCT TCATTCCCTT CTCCAAGGAG 
CAGCTGCTCC GCCTCCTAAT ACAGGCCTTA TATGACCCCA TCAACCCTGA CAGGGAGACC 
CTCGATCAGC CATCACTAAC GGATCCCCAG CGTCTGTCTA ATGAGCAGGA GGTGCTTCGG 
GCTCTGGAGC CCCTGCTGGC CCAGGCCAAC TTCTCCCCGC TGTCTGAGGA CACCCTGGCC 
TACGCGCTGG TGGTCCACCA CCCTCAGGAT GAGGTCCAGG TGACAGTAAA TTTGGATCAG 
TATGTCTACA TTCACTTCTG GGCCCTGGGC CAGCGAGTCG GGCAGATGCC CCTGAAGTCC 
AGCGTGGGCT CCAGGCGTGG CTTCTTCACC AAGCTGCCCC CTGCGGAGAG GAGATACTTT 
AAGCGGGTGG TCCTGGCAGC CCGGACCAAA CGGGGACACC TGGTACTGAA GAGTTTCAAG 
GACACGCCGC TGGAAGGCCT GGAGCAGCTG CTGCCGGAGC TGAAGGTGCG CACGCCCACC 
CTGCAGCGCG CCCTGCTCAA CCTCATGCTG GTAGTCTCCG GCGTGGCGAT CTTCGTCAAC 
GTGGGCATGG TGGTGCTAAC CGACCTCAAG GTGGCCACCT CCCTGCTGCT GCTGCTCTTC 
GCCATCTTCA TGGGCCTGCG GGCCTCCAAG ATGTTCGGGC AGCGGCGCAG CGCGCAGGCG 
TTGGAGCTGG CGCACATGCT GTACTATCGC AGTACGTCCA ACAACTCGGA GCTGCTCAGC 
GCCCTGGCCC TGCGCGCGCA GGACGAGCAC ACCAAGGAGG CGCTGCTGGC TCACAGCTTC 
CTGGCCCGGC GGCCAGGGGG CACTCAAGGC TCGCCCGAAG AGACCTCCAG GTGGCTCCGG 
TCGGAGGTGG AGAACTGGCT CCTAGCCAAG TCAGGCTGTG AGGTGACCTT CAACGGAACT 
CGGGCCCTGG CGCATCTGCA GGCCCTGACC CCCAGCATGG GATTGTACCC ACCCCCGGGT 
TTCCCCAAAC TAGACCCAGT GGCTCCGATC ACTTCCGAGC CCCCGCAAGC CACGCCCAGC 
AGTAACATCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM143 ( NM_001303538.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001290467.1 Homo sapiens transmembrane protein 143 (TMEM143), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu69248
Clone ID Related Accession (Same CDS sequence)	NM_001303538.2 , NM_001303538.1
Accession Version	NM_001303538.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	transmembrane protein 143 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC357566.1, AC011514.3, AK297610.1, AC008392.6 and AW136519.1. Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (b) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK297610.1, SRR1660805.100487.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153932, SAMEA2154405 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAGTCG AGCTTTGGCT AAGGCTCCGG GGAAAGGGTC TAGCCATGCT GCATGTGACC 
CGGGGGGTCT GGGGGTCCAG GGTCCGAGTA TGGCCACTGT TGCCCGCGCT CCTCGGGCCC 
CCCCGGGCCC TCTCATCGCT GGCAGCCAAA ATGGGGGAGT ATCGCAAGAT GTGGAACCCC 
AGGGAGCCCC GCGACTGGGC CCAGCAGTAC CGCGAGCGCT TCATTCCCTT CTCCAAGGAG 
CAGCTGCTCC GCCTCCTAAT ACAGGCCTTA TATGACCCCA TCAACCCTGA CAGGGAGACC 
CTCGATCAGC CATCACTAAC GGATCCCCAG CGTCTGTCTA ATGAGCAGGA GGTGCTTCGG 
GCTCTGGAGC CCCTGCTGGC CCAGGCCAAC TTCTCCCCGC TGTCTGAGGA CACCCTGGCC 
TACGCGCTGG TGGTCCACCA CCCTCAGGAT GAGGTCCAGG TGACAGTAAA TTTGGATCAG 
TATGTCTACA TTCACTTCTG GGCCCTGGGC CAGCGAGTCG GGCAGATGCC CCTGAAGTCC 
AGCGTGGGCT CCAGGCGTGG CTTCTTCACC AAGCTGCCCC CTGCGGAGAG GAGATACTTT 
AAGCGGGTGG TCCTGGCAGC CCGGACCAAA CGGGGACACC TGGTACTGAA GAGTTTCAAG 
GACACGCCGC TGGAAGGCCT GGAGCAGCTG CTGCCGGAGC TGAAGGTGCG CACGCCCACC 
CTGCAGCGCG CCCTGCTCAA CCTCATGCTG GTAGTCTCCG GCGTGGCGAT CTTCGTCAAC 
GTGGGCATGG TGGTGCTAAC CGACCTCAAG GTGGCCACCT CCCTGCTGCT GCTGCTCTTC 
GCCATCTTCA TGGGCCTGCG GGCCTCCAAG ATGTTCGGGC AGCGGCGCAG CGCGCAGGCG 
TTGGAGCTGG CGCACATGCT GTACTATCGC AGTACGTCCA ACAACTCGGA GCTGCTCAGC 
GCCCTGGCCC TGCGCGCGCA GGACGAGCAC ACCAAGGAGG CGCTGCTGGC TCACAGCTTC 
CTGGCCCGGC GGCCAGGGGG CACTCAAGGC TCGCCCGAAG AGACCTCCAG GTGGCTCCGG 
TCGGAGGTGG AGAACTGGCT CCTAGCCAAG TCAGGCTGTG AGGTGACCTT CAACGGAACT 
CGGGCCCTGG CGCATCTGCA GGCCCTGACC CCCAGCATGG GATTGTACCC ACCCCCGGGT 
TTCCCCAAAC TAGACCCAGT GGCTCCGATC ACTTCCGAGC CCCCGCAAGC CACGCCCAGC 
AGTAACATCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001290467.1
CDS	318..1592
Translation	MTVELWLRLRGKGLAMLHVTRGVWGSRVRVWPLLPALLGPPRALSSLAAKMGEYRKMWNPREPRDWAQQYRERFIPFSKEQLLRLLIQALYDPINPDRETLDQPSLTDPQRLSNEQEVLRALEPLLAQANFSPLSEDTLAYALVVHHPQDEVQVTVNLDQYVYIHFWALGQRVGQMPLKSSVGSRRGFFTKLPPAERRYFKRVVLAARTKRGHLVLKSFKDTPLEGLEQLLPELKVRTPTLQRALLNLMLVVSGVAIFVNVGMVVLTDLKVATSLLLLLFAIFMGLRASKMFGQRRSAQALELAHMLYYRSTSNNSELLSALALRAQDEHTKEALLAHSFLARRPGGTQGSPEETSRWLRSEVENWLLAKSGCEVTFNGTRALAHLQALTPSMGLYPPPGFPKLDPVAPITSEPPQATPSSNIS

Target ORF information:

RefSeq Version	NM_001303538.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 143 (TMEM143), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001303538.1

ORF Insert Sequence:

ATGACAGTCG AGCTTTGGCT AAGGCTCCGG GGAAAGGGTC TAGCCATGCT GCATGTGACC 
CGGGGGGTCT GGGGGTCCAG GGTCCGAGTA TGGCCACTGT TGCCCGCGCT CCTCGGGCCC 
CCCCGGGCCC TCTCATCGCT GGCAGCCAAA ATGGGGGAGT ATCGCAAGAT GTGGAACCCC 
AGGGAGCCCC GCGACTGGGC CCAGCAGTAC CGCGAGCGCT TCATTCCCTT CTCCAAGGAG 
CAGCTGCTCC GCCTCCTAAT ACAGGCCTTA TATGACCCCA TCAACCCTGA CAGGGAGACC 
CTCGATCAGC CATCACTAAC GGATCCCCAG CGTCTGTCTA ATGAGCAGGA GGTGCTTCGG 
GCTCTGGAGC CCCTGCTGGC CCAGGCCAAC TTCTCCCCGC TGTCTGAGGA CACCCTGGCC 
TACGCGCTGG TGGTCCACCA CCCTCAGGAT GAGGTCCAGG TGACAGTAAA TTTGGATCAG 
TATGTCTACA TTCACTTCTG GGCCCTGGGC CAGCGAGTCG GGCAGATGCC CCTGAAGTCC 
AGCGTGGGCT CCAGGCGTGG CTTCTTCACC AAGCTGCCCC CTGCGGAGAG GAGATACTTT 
AAGCGGGTGG TCCTGGCAGC CCGGACCAAA CGGGGACACC TGGTACTGAA GAGTTTCAAG 
GACACGCCGC TGGAAGGCCT GGAGCAGCTG CTGCCGGAGC TGAAGGTGCG CACGCCCACC 
CTGCAGCGCG CCCTGCTCAA CCTCATGCTG GTAGTCTCCG GCGTGGCGAT CTTCGTCAAC 
GTGGGCATGG TGGTGCTAAC CGACCTCAAG GTGGCCACCT CCCTGCTGCT GCTGCTCTTC 
GCCATCTTCA TGGGCCTGCG GGCCTCCAAG ATGTTCGGGC AGCGGCGCAG CGCGCAGGCG 
TTGGAGCTGG CGCACATGCT GTACTATCGC AGTACGTCCA ACAACTCGGA GCTGCTCAGC 
GCCCTGGCCC TGCGCGCGCA GGACGAGCAC ACCAAGGAGG CGCTGCTGGC TCACAGCTTC 
CTGGCCCGGC GGCCAGGGGG CACTCAAGGC TCGCCCGAAG AGACCTCCAG GTGGCTCCGG 
TCGGAGGTGG AGAACTGGCT CCTAGCCAAG TCAGGCTGTG AGGTGACCTT CAACGGAACT 
CGGGCCCTGG CGCATCTGCA GGCCCTGACC CCCAGCATGG GATTGTACCC ACCCCCGGGT 
TTCCCCAAAC TAGACCCAGT GGCTCCGATC ACTTCCGAGC CCCCGCAAGC CACGCCCAGC 
AGTAACATCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM143 ( NM_001303540.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001290469.1 Homo sapiens transmembrane protein 143 (TMEM143), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu69249
Clone ID Related Accession (Same CDS sequence)	NM_001303540.1 , NM_001303540.2
Accession Version	NM_001303540.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	transmembrane protein 143 isoform d
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC357566.1, AC011514.3, AY358161.1, AC008392.6 and AW136519.1. Transcript Variant: This variant (4) lacks two in-frame exons in the 5' coding region compared to variant 1. The encoded isoform (d) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY358161.1, AK291521.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAGTCG AGCTTTGGCT AAGGCTCCGG GGAAAGGGTC TAGCCATGCT GCATGTGACC 
CGGGGGGTCT GGGGGTCCAG GGTCCGAGTA TGGCCACTGT TGCCCGCGCT CCTCGGGCCC 
CCCCGGGCCC TCTCATCGCT GGCAGCCAAA ATGGGGGAGT ATCGCAAGAT GTGGAACCCC 
AGGGAGCCCC GCGACTGGGC CCAGCAGTAC CGCGAGCGCT TCATTCCCTT CTCCAAGGAG 
CAGCTGCTCC GCCTCCTAAT ACAGGTGACA GTAAATTTGG ATCAGTATGT CTACATTCAC 
TTCTGGGCCC TGGGCCAGCG AGTCGGGCAG ATGCCCCTGA AGTCCAGCGT GGGCTCCAGG 
CGTGGCTTCT TCACCAAGCT GCCCCCTGCG GAGAGGAGAT ACTTTAAGCG GGTGGTCCTG 
GCAGCCCGGA CCAAACGGGG ACACCTGGTA CTGAAGAGTT TCAAGGACAC GCCGCTGGAA 
GGCCTGGAGC AGCTGCTGCC GGAGCTGAAG GTGCGCACGC CCACCCTGCA GCGCGCCCTG 
CTCAACCTCA TGCTGGTAGT CTCCGGCGTG GCGATCTTCG TCAACGTGGG CATGGTGGTG 
CTAACCGACC TCAAGGTGGC CACCTCCCTG CTGCTGCTGC TCTTCGCCAT CTTCATGGGC 
CTGCGGGCCT CCAAGATGTT CGGGCAGCGG CGCAGCGCGC AGGCGTTGGA GCTGGCGCAC 
ATGCTGTACT ATCGCAGTAC GTCCAACAAC TCGGAGCTGC TCAGCGCCCT GGCCCTGCGC 
GCGCAGGACG AGCACACCAA GGAGGCGCTG CTGGCTCACA GCTTCCTGGC CCGGCGGCCA 
GGGGGCACTC AAGGCTCGCC CGAAGAGACC TCCAGGTGGC TCCGGTCGGA GGTGGAGAAC 
TGGCTCCTAG CCAAGTCAGG CTGTGAGGTG ACCTTCAACG GAACTCGGGC CCTGGCGCAT 
CTGCAGGCCC TGACCCCCAG CATGGGATTG TACCCACCCC CGGGTTTCCC CAAACTAGAC 
CCAGTGGCTC CGATCACTTC CGAGCCCCCG CAAGCCACGC CCAGCAGTAA CATCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001290469.1
CDS	318..1397
Translation	MTVELWLRLRGKGLAMLHVTRGVWGSRVRVWPLLPALLGPPRALSSLAAKMGEYRKMWNPREPRDWAQQYRERFIPFSKEQLLRLLIQVTVNLDQYVYIHFWALGQRVGQMPLKSSVGSRRGFFTKLPPAERRYFKRVVLAARTKRGHLVLKSFKDTPLEGLEQLLPELKVRTPTLQRALLNLMLVVSGVAIFVNVGMVVLTDLKVATSLLLLLFAIFMGLRASKMFGQRRSAQALELAHMLYYRSTSNNSELLSALALRAQDEHTKEALLAHSFLARRPGGTQGSPEETSRWLRSEVENWLLAKSGCEVTFNGTRALAHLQALTPSMGLYPPPGFPKLDPVAPITSEPPQATPSSNIS

Target ORF information:

RefSeq Version	NM_001303540.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 143 (TMEM143), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001303540.1

ORF Insert Sequence:

ATGACAGTCG AGCTTTGGCT AAGGCTCCGG GGAAAGGGTC TAGCCATGCT GCATGTGACC 
CGGGGGGTCT GGGGGTCCAG GGTCCGAGTA TGGCCACTGT TGCCCGCGCT CCTCGGGCCC 
CCCCGGGCCC TCTCATCGCT GGCAGCCAAA ATGGGGGAGT ATCGCAAGAT GTGGAACCCC 
AGGGAGCCCC GCGACTGGGC CCAGCAGTAC CGCGAGCGCT TCATTCCCTT CTCCAAGGAG 
CAGCTGCTCC GCCTCCTAAT ACAGGTGACA GTAAATTTGG ATCAGTATGT CTACATTCAC 
TTCTGGGCCC TGGGCCAGCG AGTCGGGCAG ATGCCCCTGA AGTCCAGCGT GGGCTCCAGG 
CGTGGCTTCT TCACCAAGCT GCCCCCTGCG GAGAGGAGAT ACTTTAAGCG GGTGGTCCTG 
GCAGCCCGGA CCAAACGGGG ACACCTGGTA CTGAAGAGTT TCAAGGACAC GCCGCTGGAA 
GGCCTGGAGC AGCTGCTGCC GGAGCTGAAG GTGCGCACGC CCACCCTGCA GCGCGCCCTG 
CTCAACCTCA TGCTGGTAGT CTCCGGCGTG GCGATCTTCG TCAACGTGGG CATGGTGGTG 
CTAACCGACC TCAAGGTGGC CACCTCCCTG CTGCTGCTGC TCTTCGCCAT CTTCATGGGC 
CTGCGGGCCT CCAAGATGTT CGGGCAGCGG CGCAGCGCGC AGGCGTTGGA GCTGGCGCAC 
ATGCTGTACT ATCGCAGTAC GTCCAACAAC TCGGAGCTGC TCAGCGCCCT GGCCCTGCGC 
GCGCAGGACG AGCACACCAA GGAGGCGCTG CTGGCTCACA GCTTCCTGGC CCGGCGGCCA 
GGGGGCACTC AAGGCTCGCC CGAAGAGACC TCCAGGTGGC TCCGGTCGGA GGTGGAGAAC 
TGGCTCCTAG CCAAGTCAGG CTGTGAGGTG ACCTTCAACG GAACTCGGGC CCTGGCGCAT 
CTGCAGGCCC TGACCCCCAG CATGGGATTG TACCCACCCC CGGGTTTCCC CAAACTAGAC 
CCAGTGGCTC CGATCACTTC CGAGCCCCCG CAAGCCACGC CCAGCAGTAA CATCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM143 ( NM_001303540.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001290469.1 Homo sapiens transmembrane protein 143 (TMEM143), transcript variant 4, mRNA.

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CloneID	OHu69249
Clone ID Related Accession (Same CDS sequence)	NM_001303540.1 , NM_001303540.2
Accession Version	NM_001303540.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	transmembrane protein 143 isoform d
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC357566.1, AY358161.1, AC008392.6 and AW136519.1. On May 31, 2019 this sequence version replaced NM_001303540.1. Transcript Variant: This variant (4) lacks two in-frame exons in the 5' coding region compared to variant 1. The encoded isoform (d) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY358161.1, AK291521.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAGTCG AGCTTTGGCT AAGGCTCCGG GGAAAGGGTC TAGCCATGCT GCATGTGACC 
CGGGGGGTCT GGGGGTCCAG GGTCCGAGTA TGGCCACTGT TGCCCGCGCT CCTCGGGCCC 
CCCCGGGCCC TCTCATCGCT GGCAGCCAAA ATGGGGGAGT ATCGCAAGAT GTGGAACCCC 
AGGGAGCCCC GCGACTGGGC CCAGCAGTAC CGCGAGCGCT TCATTCCCTT CTCCAAGGAG 
CAGCTGCTCC GCCTCCTAAT ACAGGTGACA GTAAATTTGG ATCAGTATGT CTACATTCAC 
TTCTGGGCCC TGGGCCAGCG AGTCGGGCAG ATGCCCCTGA AGTCCAGCGT GGGCTCCAGG 
CGTGGCTTCT TCACCAAGCT GCCCCCTGCG GAGAGGAGAT ACTTTAAGCG GGTGGTCCTG 
GCAGCCCGGA CCAAACGGGG ACACCTGGTA CTGAAGAGTT TCAAGGACAC GCCGCTGGAA 
GGCCTGGAGC AGCTGCTGCC GGAGCTGAAG GTGCGCACGC CCACCCTGCA GCGCGCCCTG 
CTCAACCTCA TGCTGGTAGT CTCCGGCGTG GCGATCTTCG TCAACGTGGG CATGGTGGTG 
CTAACCGACC TCAAGGTGGC CACCTCCCTG CTGCTGCTGC TCTTCGCCAT CTTCATGGGC 
CTGCGGGCCT CCAAGATGTT CGGGCAGCGG CGCAGCGCGC AGGCGTTGGA GCTGGCGCAC 
ATGCTGTACT ATCGCAGTAC GTCCAACAAC TCGGAGCTGC TCAGCGCCCT GGCCCTGCGC 
GCGCAGGACG AGCACACCAA GGAGGCGCTG CTGGCTCACA GCTTCCTGGC CCGGCGGCCA 
GGGGGCACTC AAGGCTCGCC CGAAGAGACC TCCAGGTGGC TCCGGTCGGA GGTGGAGAAC 
TGGCTCCTAG CCAAGTCAGG CTGTGAGGTG ACCTTCAACG GAACTCGGGC CCTGGCGCAT 
CTGCAGGCCC TGACCCCCAG CATGGGATTG TACCCACCCC CGGGTTTCCC CAAACTAGAC 
CCAGTGGCTC CGATCACTTC CGAGCCCCCG CAAGCCACGC CCAGCAGTAA CATCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001290469.1
CDS	21..1100
Translation	MTVELWLRLRGKGLAMLHVTRGVWGSRVRVWPLLPALLGPPRALSSLAAKMGEYRKMWNPREPRDWAQQYRERFIPFSKEQLLRLLIQVTVNLDQYVYIHFWALGQRVGQMPLKSSVGSRRGFFTKLPPAERRYFKRVVLAARTKRGHLVLKSFKDTPLEGLEQLLPELKVRTPTLQRALLNLMLVVSGVAIFVNVGMVVLTDLKVATSLLLLLFAIFMGLRASKMFGQRRSAQALELAHMLYYRSTSNNSELLSALALRAQDEHTKEALLAHSFLARRPGGTQGSPEETSRWLRSEVENWLLAKSGCEVTFNGTRALAHLQALTPSMGLYPPPGFPKLDPVAPITSEPPQATPSSNIS

Target ORF information:

RefSeq Version	NM_001303540.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 143 (TMEM143), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001303540.2

ORF Insert Sequence:

ATGACAGTCG AGCTTTGGCT AAGGCTCCGG GGAAAGGGTC TAGCCATGCT GCATGTGACC 
CGGGGGGTCT GGGGGTCCAG GGTCCGAGTA TGGCCACTGT TGCCCGCGCT CCTCGGGCCC 
CCCCGGGCCC TCTCATCGCT GGCAGCCAAA ATGGGGGAGT ATCGCAAGAT GTGGAACCCC 
AGGGAGCCCC GCGACTGGGC CCAGCAGTAC CGCGAGCGCT TCATTCCCTT CTCCAAGGAG 
CAGCTGCTCC GCCTCCTAAT ACAGGTGACA GTAAATTTGG ATCAGTATGT CTACATTCAC 
TTCTGGGCCC TGGGCCAGCG AGTCGGGCAG ATGCCCCTGA AGTCCAGCGT GGGCTCCAGG 
CGTGGCTTCT TCACCAAGCT GCCCCCTGCG GAGAGGAGAT ACTTTAAGCG GGTGGTCCTG 
GCAGCCCGGA CCAAACGGGG ACACCTGGTA CTGAAGAGTT TCAAGGACAC GCCGCTGGAA 
GGCCTGGAGC AGCTGCTGCC GGAGCTGAAG GTGCGCACGC CCACCCTGCA GCGCGCCCTG 
CTCAACCTCA TGCTGGTAGT CTCCGGCGTG GCGATCTTCG TCAACGTGGG CATGGTGGTG 
CTAACCGACC TCAAGGTGGC CACCTCCCTG CTGCTGCTGC TCTTCGCCAT CTTCATGGGC 
CTGCGGGCCT CCAAGATGTT CGGGCAGCGG CGCAGCGCGC AGGCGTTGGA GCTGGCGCAC 
ATGCTGTACT ATCGCAGTAC GTCCAACAAC TCGGAGCTGC TCAGCGCCCT GGCCCTGCGC 
GCGCAGGACG AGCACACCAA GGAGGCGCTG CTGGCTCACA GCTTCCTGGC CCGGCGGCCA 
GGGGGCACTC AAGGCTCGCC CGAAGAGACC TCCAGGTGGC TCCGGTCGGA GGTGGAGAAC 
TGGCTCCTAG CCAAGTCAGG CTGTGAGGTG ACCTTCAACG GAACTCGGGC CCTGGCGCAT 
CTGCAGGCCC TGACCCCCAG CATGGGATTG TACCCACCCC CGGGTTTCCC CAAACTAGAC 
CCAGTGGCTC CGATCACTTC CGAGCCCCCG CAAGCCACGC CCAGCAGTAA CATCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Diabetes care33(10)2250-3(2010 Oct)
Bailey SD,Xie C,Do R,Montpetit A,Diaz R,Mohan V,Keavney B,Yusuf S,Gerstein HC,Engert JC,Anand S,

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.
American journal of human genetics85(5)628-42(2009 Nov)
Talmud PJ,Drenos F,Shah S,Shah T,Palmen J,Verzilli C,Gaunt TR,Pallas J,Lovering R,Li K,Casas JP,Sofat R,Kumari M,Rodriguez S,Johnson T,Newhouse SJ,Dominiczak A,Samani NJ,Caulfield M,Sever P,Stanton A,Shields DC,Padmanabhan S,Melander O,Hastie C,Delles C,Ebrahim S,Marmot MG,Smith GD,Lawlor DA,Munroe PB,Day IN,Kivimaki M,Whittaker J,Humphries SE,Hingorani AD,,,

The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.
Genome research13(10)2265-70(2003 Oct)
Clark HF,Gurney AL,Abaya E,Baker K,Baldwin D,Brush J,Chen J,Chow B,Chui C,Crowley C,Currell B,Deuel B,Dowd P,Eaton D,Foster J,Grimaldi C,Gu Q,Hass PE,Heldens S,Huang A,Kim HS,Klimowski L,Jin Y,Johnson S,Lee J,Lewis L,Liao D,Mark M,Robbie E,Sanchez C,Schoenfeld J,Seshagiri S,Simmons L,Singh J,Smith V,Stinson J,Vagts A,Vandlen R,Watanabe C,Wieand D,Woods K,Xie MH,Yansura D,Yi S,Yu G,Yuan J,Zhang M,Zhang Z,Goddard A,Wood WI,Godowski P,Gray A

Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing.
EMBO reports1(3)287-92(2000 Sep)
Simpson JC,Wellenreuther R,Poustka A,Pepperkok R,Wiemann S

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TMEM143 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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