UBA6 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBA6
Entrez Gene ID	55236
Full Name	ubiquitin like modifier activating enzyme 6
Synonyms	E1-L2,MOP-4,UBE1L2
General protein information	Preferred Names ubiquitin like modifier activating enzyme 6 Names ubiquitin-like modifier-activating enzyme 6 UBA6, ubiquitin-activating enzyme E1 monocyte protein 4 ubiquitin-activating enzyme 6 ubiquitin-activating enzyme E1-like 2 ubiquitin-activating enzyme E1-like protein 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q13.2
Summary	Modification of proteins with ubiquitin (UBB; MIM 191339) or ubiquitin-like proteins controls many signaling networks and requires a ubiquitin-activating enzyme (E1), a ubiquitin conjugating enzyme (E2), and a ubiquitin protein ligase (E3). UBE1L2 is an E1 enzyme that initiates the activation and conjugation of ubiquitin-like proteins (Jin et al., 2007 [PubMed 17597759]).[supplied by OMIM, Mar 2008].
Disorder MIM:	611361

mRNA and Protein(s)

mRNA	Protein	Name
NM_018227.6	NP_060697.4	ubiquitin-like modifier-activating enzyme 6
XM_017008359.1	XP_016863848.1	ubiquitin-like modifier-activating enzyme 6 isoform X1
XM_017008359.2	XP_016863848.1	ubiquitin-like modifier-activating enzyme 6 isoform X1
NM_018227.5	NP_060697.4	ubiquitin-like modifier-activating enzyme 6

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UBA6	XP_001164227.2
Mus musculus (house mouse)	Uba6	NP_766300.1
Danio rerio (zebrafish)	si:dkey-82j4.2	XP_695755.4
Rattus norvegicus (Norway rat)	Uba6	NP_001100683.1
Xenopus tropicalis (tropical clawed frog)	uba6	XP_004911132.1
Homo sapiens (human)	UBA6	NP_060697.4
Bos taurus (cattle)	UBA6	NP_001076907.1
Gallus gallus (chicken)	UBA6	XP_420609.4
	UBA6	XP_002804149.1
Canis lupus familiaris (dog)	UBA6	XP_532390.2

Gene Ontology
Bibliography

Related articles in PubMed

Orthogonal ubiquitin transfer identifies ubiquitination substrates under differential control by the two ubiquitin activating enzymes.
Liu X, Zhao B, Sun L, Bhuripanyo K, Wang Y, Bi Y, Davuluri RV, Duong DM, Nanavati D, Yin J, Kiyokawa H
Nature communications814286(2017 Jan)

LMO2 blocks the UBA6-USE1 interaction and downstream FAT10ylation by targeting the ubiquitin fold domain of UBA6.
Wu C, Liu Y, Gu X, Zhu T, Yang S, Sun W
Biochemical and biophysical research communications478(3)1442-8(2016 09)

Interstitial microdeletions including the chromosome band 4q13.2 and the UBA6 gene as possible causes of intellectual disability and behavior disorder.
Quintela I, Barros F, Fernandez-Prieto M, Martinez-Regueiro R, Castro-Gago M, Carracedo A, Gomez-Lado C, Eiris J
American journal of medical genetics. Part A167A(12)3113-20(2015 Dec)

Disruption of FAT10-MAD2 binding inhibits tumor progression.
Theng SS, Wang W, Mah WC, Chan C, Zhuo J, Gao Y, Qin H, Lim L, Chong SS, Song J, Lee CG
Proceedings of the National Academy of Sciences of the United States of America111(49)E5282-91(2014 Dec)

The Escherichia coli adherence factor plasmid of enteropathogenic Escherichia coli causes a global decrease in ubiquitylated host cell proteins by decreasing ubiquitin E1 enzyme expression through host aspartyl proteases.
Lin AE, Guttman JA
The international journal of biochemistry & cell biology44(12)2223-32(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study revealed a novel function of LMO2 involving in the regulatory hierarchy of UBA6-USE1-FAT10ylation pathway by targeting the E1 enzyme UBA6.
Title: LMO2 blocks the UBA6-USE1 interaction and downstream FAT10ylation by targeting the ubiquitin fold domain of UBA6.

Interstitial microdeletions including the chromosome band 4q13.2 and the UBA6 gene as possible causes of intellectual disability and behavior disorder.
Title: Interstitial microdeletions including the chromosome band 4q13.2 and the UBA6 gene as possible causes of intellectual disability and behavior disorder.

Diminished ubiquitylation phenotype observed in enteropathogenic Escherichia coli infected cells corresponds to a strong reduction in the abundance of both Ube1 and Uba6.
Title: The Escherichia coli adherence factor plasmid of enteropathogenic Escherichia coli causes a global decrease in ubiquitylated host cell proteins by decreasing ubiquitin E1 enzyme expression through host aspartyl proteases.

Data indicate the potential role of cytokine-induced FAT10 expression in regulating Uba6 pathways.
Title: Mechanistic studies on activation of ubiquitin and di-ubiquitin-like protein, FAT10, by ubiquitin-like modifier activating enzyme 6, Uba6.

Ubiquitin-like modifier activating enzyme 6 activates not only ubiquitin, but also the ubiquitin-like modifier FAT10[review]
Title: Activating the ubiquitin family: UBA6 challenges the field.

The following UBA6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBA6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02738	NM_018227.6 Latest version!	Homo sapiens ubiquitin like modifier activating enzyme 6 (UBA6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu95274	XM_017008359.2 Latest version!	Homo sapiens ubiquitin like modifier activating enzyme 6 (UBA6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OHu95274	XM_017008359.1	Homo sapiens ubiquitin like modifier activating enzyme 6 (UBA6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu02738	NM_018227.5	Homo sapiens ubiquitin like modifier activating enzyme 6 (UBA6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBA6 ( NM_018227.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_060697.4 Homo sapiens ubiquitin like modifier activating enzyme 6 (UBA6), mRNA.

Price & Availability↑

CloneID	OHu02738
Clone ID Related Accession (Same CDS sequence)	NM_018227.6 , NM_018227.5
Accession Version	NM_018227.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Homo sapiens(human)
Product	ubiquitin-like modifier-activating enzyme 6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC126484.1, AY359880.1, AL832015.2, AW451445.1, BM973988.1 and AC096720.3. On Nov 22, 2018 this sequence version replaced NM_018227.5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.92861.1, AL832015.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000322244.10/ ENSP00000313454.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAAGGAT CCGAGCCTGT GGCCGCCCAT CAGGGGGAAG AGGCGTCCTG TTCTTCCTGG 
GGGACTGGCA GCACAAATAA AAATTTGCCC ATTATGTCAA CAGCATCTGT GGAAATCGAT 
GATGCATTGT ATAGTCGACA GAGGTACGTT CTTGGAGACA CAGCAATGCA GAAGATGGCC 
AAGTCCCATG TTTTCTTAAG TGGGATGGGT GGTCTTGGTT TGGAAATTGC AAAGAATCTT 
GTTCTTGCAG GGATTAAGGC AGTTACAATT CATGATACAG AAAAATGCCA AGCATGGGAT 
CTAGGAACCA ACTTCTTTCT CAGTGAAGAT GATGTTGTTA ATAAGAGAAA CAGGGCTGAA 
GCTGTACTTA AACATATTGC AGAACTAAAT CCATACGTTC ATGTCACATC ATCTTCTGTT 
CCTTTCAATG AGACCACAGA TCTCTCCTTT TTAGATAAAT ACCAGTGTGT AGTATTGACT 
GAGATGAAAC TTCCATTGCA GAAGAAGATC AATGACTTTT GCCGTTCTCA GTGCCCTCCA 
ATTAAGTTTA TCAGTGCAGA TGTACATGGA ATTTGGTCAA GGTTATTTTG TGATTTCGGT 
GATGAATTTG AAGTTTTAGA TACAACAGGA GAAGAACCAA AAGAAATTTT CATTTCAAAC 
ATAACGCAAG CAAATCCTGG CATTGTTACT TGCCTTGAAA ATCATCCTCA CAAACTGGAG 
ACAGGACAAT TCCTAACATT TCGAGAAATT AATGGAATGA CAGGTTTAAA TGGATCTATA 
CAACAAATAA CGGTGATATC GCCATTTTCT TTTAGTATTG GTGACACCAC AGAACTGGAA 
CCATATTTAC ATGGAGGCAT AGCTGTCCAA GTTAAGACTC CTAAAACAGT TTTTTTTGAA 
TCACTGGAGA GGCAGTTAAA ACATCCAAAG TGCCTTATTG TGGATTTTAG CAACCCTGAG 
GCACCTTTAG AGATTCACAC AGCTATGCTT GCCTTGGACC AGTTTCAGGA GAAATACAGT 
CGCAAGCCAA ATGTTGGATG CCAACAAGAT TCAGAAGAAC TGTTGAAACT AGCAACATCT 
ATAAGTGAAA CCTTGGAAGA GAAGCCTGAT GTAAATGCTG ACATTGTGCA TTGGCTCTCT 
TGGACTGCCC AAGGCTTTTT ATCTCCACTT GCTGCAGCAG TAGGAGGTGT TGCCAGCCAA 
GAAGTATTGA AAGCTGTAAC AGGAAAATTT TCTCCTTTGT GCCAGTGGTT ATATCTTGAA 
GCAGCAGATA TTGTTGAATC ACTAGGCAAA CCTGAATGTG AAGAATTTCT CCCACGAGGA 
GATAGATATG ATGCCTTAAG AGCTTGCATT GGAGACACTT TGTGTCAGAA ACTGCAAAAT 
TTAAACATCT TCTTAGTAGG GTGTGGAGCC ATAGGCTGTG AAATGTTGAA AAATTTTGCT 
TTACTTGGTG TTGGCACAAG CAAAGAGAAA GGAATGATTA CAGTTACAGA TCCTGACTTG 
ATAGAGAAAT CCAACTTAAA TAGACAGTTC CTATTTCGTC CTCATCACAT ACAGAAACCT 
AAAAGCTACA CTGCTGCTGA TGCTACTCTG AAAATAAATT CTCAAATAAA GATAGATGCA 
CACCTGAACA AAGTATGTCC AACCACTGAG ACCATTTACA ATGATGAGTT CTATACTAAA 
CAAGATGTAA TTATTACAGC ATTAGATAAT GTGGAAGCCA GGAGATACGT AGACAGTCGT 
TGCTTAGCAA ATCTAAGGCC TCTTTTAGAT TCTGGAACAA TGGGCACTAA GGGACACACT 
GAAGTTATTG TACCGCATTT GACTGAGTCT TACAATAGTC ATCGGGATCC CCCAGAAGAG 
GAAATACCAT TTTGTACTCT AAAATCCTTT CCAGCTGCTA TTGAACATAC CATACAGTGG 
GCAAGAGATA AGTTTGAAAG TTCCTTTTCC CACAAACCTT CATTGTTTAA CAAATTTTGG 
CAAACCTATT CATCTGCAGA AGAAGTCTTA CAGAAGATAC AGAGTGGACA CAGTTTAGAA 
GGCTGTTTTC AAGTTATAAA GTTACTTAGC AGAAGACCTA GAAATTGGTC CCAGTGTGTA 
GAATTAGCAA GATTAAAGTT TGAAAAATAT TTTAACCATA AGGCTCTTCA GCTTCTTCAC 
TGTTTCCCTC TGGACATACG ATTAAAAGAT GGCAGTTTAT TTTGGCAGTC ACCAAAGAGG 
CCACCCTCTC CAATAAAATT TGATTTAAAT GAGCCTTTGC ACCTCAGTTT CCTTCAGAAT 
GCTGCAAAAC TATATGCTAC AGTATATTGT ATTCCATTTG CAGAAGAGGA CTTATCAGCA 
GATGCCCTCT TGAATATTCT TTCAGAAGTA AAGATTCAGG AATTCAAGCC TTCCAATAAG 
GTTGTTCAAA CAGATGAAAC TGCAAGGAAA CCAGACCATG TTCCTATTAG CAGTGAAGAT 
GAGAGGAATG CAATTTTCCA ACTAGAAAAG GCTATTTTAT CTAATGAAGC CACCAAAAGT 
GACCTTCAGA TGGCAGTGCT TTCATTTGAA AAAGATGATG ATCATAATGG ACACATAGAT 
TTCATCACAG CTGCATCAAA TCTTCGTGCC AAAATGTACA GCATTGAACC AGCTGACCGT 
TTCAAAACAA AGCGCATAGC TGGTAAAATT ATACCTGCTA TAGCAACAAC CACTGCTACA 
GTTTCTGGCT TGGTTGCCTT GGAGATGATC AAAGTAACTG GTGGCTATCC ATTTGAAGCT 
TACAAAAATT GTTTTCTTAA CTTAGCCATT CCAATTGTAG TATTTACAGA GACAACTGAA 
GTAAGGAAAA CTAAAATCAG AAATGGAATA TCATTTACAA TTTGGGATCG ATGGACCGTA 
CATGGAAAAG AAGATTTCAC CCTCTTGGAT TTCATAAATG CAGTCAAAGA GAAGTATGGA 
ATTGAGCCAA CAATGGTGGT ACAGGGAGTC AAAATGCTTT ATGTTCCTGT AATGCCTGGT 
CATGCAAAAA GATTGAAGTT AACAATGCAT AAACTTGTAA AACCTACTAC TGAAAAGAAA 
TATGTGGATC TTACTGTGTC ATTTGCTCCA GACATTGATG GAGATGAAGA TTTGCCGGGA 
CCTCCAGTAA GATACTACTT CAGTCATGAC ACTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060697.4
CDS	37..3195
Translation	MEGSEPVAAHQGEEASCSSWGTGSTNKNLPIMSTASVEIDDALYSRQRYVLGDTAMQKMAKSHVFLSGMGGLGLEIAKNLVLAGIKAVTIHDTEKCQAWDLGTNFFLSEDDVVNKRNRAEAVLKHIAELNPYVHVTSSSVPFNETTDLSFLDKYQCVVLTEMKLPLQKKINDFCRSQCPPIKFISADVHGIWSRLFCDFGDEFEVLDTTGEEPKEIFISNITQANPGIVTCLENHPHKLETGQFLTFREINGMTGLNGSIQQITVISPFSFSIGDTTELEPYLHGGIAVQVKTPKTVFFESLERQLKHPKCLIVDFSNPEAPLEIHTAMLALDQFQEKYSRKPNVGCQQDSEELLKLATSISETLEEKPDVNADIVHWLSWTAQGFLSPLAAAVGGVASQEVLKAVTGKFSPLCQWLYLEAADIVESLGKPECEEFLPRGDRYDALRACIGDTLCQKLQNLNIFLVGCGAIGCEMLKNFALLGVGTSKEKGMITVTDPDLIEKSNLNRQFLFRPHHIQKPKSYTAADATLKINSQIKIDAHLNKVCPTTETIYNDEFYTKQDVIITALDNVEARRYVDSRCLANLRPLLDSGTMGTKGHTEVIVPHLTESYNSHRDPPEEEIPFCTLKSFPAAIEHTIQWARDKFESSFSHKPSLFNKFWQTYSSAEEVLQKIQSGHSLEGCFQVIKLLSRRPRNWSQCVELARLKFEKYFNHKALQLLHCFPLDIRLKDGSLFWQSPKRPPSPIKFDLNEPLHLSFLQNAAKLYATVYCIPFAEEDLSADALLNILSEVKIQEFKPSNKVVQTDETARKPDHVPISSEDERNAIFQLEKAILSNEATKSDLQMAVLSFEKDDDHNGHIDFITAASNLRAKMYSIEPADRFKTKRIAGKIIPAIATTTATVSGLVALEMIKVTGGYPFEAYKNCFLNLAIPIVVFTETTEVRKTKIRNGISFTIWDRWTVHGKEDFTLLDFINAVKEKYGIEPTMVVQGVKMLYVPVMPGHAKRLKLTMHKLVKPTTEKKYVDLTVSFAPDIDGDEDLPGPPVRYYFSHDTD

Target ORF information:

RefSeq Version	NM_018227.6
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin like modifier activating enzyme 6 (UBA6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018227.6

ORF Insert Sequence:

ATGGAAGGAT CCGAGCCTGT GGCCGCCCAT CAGGGGGAAG AGGCGTCCTG TTCTTCCTGG 
GGGACTGGCA GCACAAATAA AAATTTGCCC ATTATGTCAA CAGCATCTGT GGAAATCGAT 
GATGCATTGT ATAGTCGACA GAGGTACGTT CTTGGAGACA CAGCAATGCA GAAGATGGCC 
AAGTCCCATG TTTTCTTAAG TGGGATGGGT GGTCTTGGTT TGGAAATTGC AAAGAATCTT 
GTTCTTGCAG GGATTAAGGC AGTTACAATT CATGATACAG AAAAATGCCA AGCATGGGAT 
CTAGGAACCA ACTTCTTTCT CAGTGAAGAT GATGTTGTTA ATAAGAGAAA CAGGGCTGAA 
GCTGTACTTA AACATATTGC AGAACTAAAT CCATACGTTC ATGTCACATC ATCTTCTGTT 
CCTTTCAATG AGACCACAGA TCTCTCCTTT TTAGATAAAT ACCAGTGTGT AGTATTGACT 
GAGATGAAAC TTCCATTGCA GAAGAAGATC AATGACTTTT GCCGTTCTCA GTGCCCTCCA 
ATTAAGTTTA TCAGTGCAGA TGTACATGGA ATTTGGTCAA GGTTATTTTG TGATTTCGGT 
GATGAATTTG AAGTTTTAGA TACAACAGGA GAAGAACCAA AAGAAATTTT CATTTCAAAC 
ATAACGCAAG CAAATCCTGG CATTGTTACT TGCCTTGAAA ATCATCCTCA CAAACTGGAG 
ACAGGACAAT TCCTAACATT TCGAGAAATT AATGGAATGA CAGGTTTAAA TGGATCTATA 
CAACAAATAA CGGTGATATC GCCATTTTCT TTTAGTATTG GTGACACCAC AGAACTGGAA 
CCATATTTAC ATGGAGGCAT AGCTGTCCAA GTTAAGACTC CTAAAACAGT TTTTTTTGAA 
TCACTGGAGA GGCAGTTAAA ACATCCAAAG TGCCTTATTG TGGATTTTAG CAACCCTGAG 
GCACCTTTAG AGATTCACAC AGCTATGCTT GCCTTGGACC AGTTTCAGGA GAAATACAGT 
CGCAAGCCAA ATGTTGGATG CCAACAAGAT TCAGAAGAAC TGTTGAAACT AGCAACATCT 
ATAAGTGAAA CCTTGGAAGA GAAGCCTGAT GTAAATGCTG ACATTGTGCA TTGGCTCTCT 
TGGACTGCCC AAGGCTTTTT ATCTCCACTT GCTGCAGCAG TAGGAGGTGT TGCCAGCCAA 
GAAGTATTGA AAGCTGTAAC AGGAAAATTT TCTCCTTTGT GCCAGTGGTT ATATCTTGAA 
GCAGCAGATA TTGTTGAATC ACTAGGCAAA CCTGAATGTG AAGAATTTCT CCCACGAGGA 
GATAGATATG ATGCCTTAAG AGCTTGCATT GGAGACACTT TGTGTCAGAA ACTGCAAAAT 
TTAAACATCT TCTTAGTAGG GTGTGGAGCC ATAGGCTGTG AAATGTTGAA AAATTTTGCT 
TTACTTGGTG TTGGCACAAG CAAAGAGAAA GGAATGATTA CAGTTACAGA TCCTGACTTG 
ATAGAGAAAT CCAACTTAAA TAGACAGTTC CTATTTCGTC CTCATCACAT ACAGAAACCT 
AAAAGCTACA CTGCTGCTGA TGCTACTCTG AAAATAAATT CTCAAATAAA GATAGATGCA 
CACCTGAACA AAGTATGTCC AACCACTGAG ACCATTTACA ATGATGAGTT CTATACTAAA 
CAAGATGTAA TTATTACAGC ATTAGATAAT GTGGAAGCCA GGAGATACGT AGACAGTCGT 
TGCTTAGCAA ATCTAAGGCC TCTTTTAGAT TCTGGAACAA TGGGCACTAA GGGACACACT 
GAAGTTATTG TACCGCATTT GACTGAGTCT TACAATAGTC ATCGGGATCC CCCAGAAGAG 
GAAATACCAT TTTGTACTCT AAAATCCTTT CCAGCTGCTA TTGAACATAC CATACAGTGG 
GCAAGAGATA AGTTTGAAAG TTCCTTTTCC CACAAACCTT CATTGTTTAA CAAATTTTGG 
CAAACCTATT CATCTGCAGA AGAAGTCTTA CAGAAGATAC AGAGTGGACA CAGTTTAGAA 
GGCTGTTTTC AAGTTATAAA GTTACTTAGC AGAAGACCTA GAAATTGGTC CCAGTGTGTA 
GAATTAGCAA GATTAAAGTT TGAAAAATAT TTTAACCATA AGGCTCTTCA GCTTCTTCAC 
TGTTTCCCTC TGGACATACG ATTAAAAGAT GGCAGTTTAT TTTGGCAGTC ACCAAAGAGG 
CCACCCTCTC CAATAAAATT TGATTTAAAT GAGCCTTTGC ACCTCAGTTT CCTTCAGAAT 
GCTGCAAAAC TATATGCTAC AGTATATTGT ATTCCATTTG CAGAAGAGGA CTTATCAGCA 
GATGCCCTCT TGAATATTCT TTCAGAAGTA AAGATTCAGG AATTCAAGCC TTCCAATAAG 
GTTGTTCAAA CAGATGAAAC TGCAAGGAAA CCAGACCATG TTCCTATTAG CAGTGAAGAT 
GAGAGGAATG CAATTTTCCA ACTAGAAAAG GCTATTTTAT CTAATGAAGC CACCAAAAGT 
GACCTTCAGA TGGCAGTGCT TTCATTTGAA AAAGATGATG ATCATAATGG ACACATAGAT 
TTCATCACAG CTGCATCAAA TCTTCGTGCC AAAATGTACA GCATTGAACC AGCTGACCGT 
TTCAAAACAA AGCGCATAGC TGGTAAAATT ATACCTGCTA TAGCAACAAC CACTGCTACA 
GTTTCTGGCT TGGTTGCCTT GGAGATGATC AAAGTAACTG GTGGCTATCC ATTTGAAGCT 
TACAAAAATT GTTTTCTTAA CTTAGCCATT CCAATTGTAG TATTTACAGA GACAACTGAA 
GTAAGGAAAA CTAAAATCAG AAATGGAATA TCATTTACAA TTTGGGATCG ATGGACCGTA 
CATGGAAAAG AAGATTTCAC CCTCTTGGAT TTCATAAATG CAGTCAAAGA GAAGTATGGA 
ATTGAGCCAA CAATGGTGGT ACAGGGAGTC AAAATGCTTT ATGTTCCTGT AATGCCTGGT 
CATGCAAAAA GATTGAAGTT AACAATGCAT AAACTTGTAA AACCTACTAC TGAAAAGAAA 
TATGTGGATC TTACTGTGTC ATTTGCTCCA GACATTGATG GAGATGAAGA TTTGCCGGGA 
CCTCCAGTAA GATACTACTT CAGTCATGAC ACTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBA6 ( NM_018227.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060697.4 Homo sapiens ubiquitin like modifier activating enzyme 6 (UBA6), mRNA.

Price & Availability↑

CloneID	OHu02738
Clone ID Related Accession (Same CDS sequence)	NM_018227.6 , NM_018227.5
Accession Version	NM_018227.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	ubiquitin-like modifier-activating enzyme 6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL596839.1, BC126484.1, AY359880.1, AL832015.2, AW451445.1 and BM973988.1. On Jul 3, 2007 this sequence version replaced NM_018227.4. ##Evidence-Data-START## Transcript exon combination :: AL832015.2, SRR1660805.124247.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGGAT CCGAGCCTGT GGCCGCCCAT CAGGGGGAAG AGGCGTCCTG TTCTTCCTGG 
GGGACTGGCA GCACAAATAA AAATTTGCCC ATTATGTCAA CAGCATCTGT GGAAATCGAT 
GATGCATTGT ATAGTCGACA GAGGTACGTT CTTGGAGACA CAGCAATGCA GAAGATGGCC 
AAGTCCCATG TTTTCTTAAG TGGGATGGGT GGTCTTGGTT TGGAAATTGC AAAGAATCTT 
GTTCTTGCAG GGATTAAGGC AGTTACAATT CATGATACAG AAAAATGCCA AGCATGGGAT 
CTAGGAACCA ACTTCTTTCT CAGTGAAGAT GATGTTGTTA ATAAGAGAAA CAGGGCTGAA 
GCTGTACTTA AACATATTGC AGAACTAAAT CCATACGTTC ATGTCACATC ATCTTCTGTT 
CCTTTCAATG AGACCACAGA TCTCTCCTTT TTAGATAAAT ACCAGTGTGT AGTATTGACT 
GAGATGAAAC TTCCATTGCA GAAGAAGATC AATGACTTTT GCCGTTCTCA GTGCCCTCCA 
ATTAAGTTTA TCAGTGCAGA TGTACATGGA ATTTGGTCAA GGTTATTTTG TGATTTCGGT 
GATGAATTTG AAGTTTTAGA TACAACAGGA GAAGAACCAA AAGAAATTTT CATTTCAAAC 
ATAACGCAAG CAAATCCTGG CATTGTTACT TGCCTTGAAA ATCATCCTCA CAAACTGGAG 
ACAGGACAAT TCCTAACATT TCGAGAAATT AATGGAATGA CAGGTTTAAA TGGATCTATA 
CAACAAATAA CGGTGATATC GCCATTTTCT TTTAGTATTG GTGACACCAC AGAACTGGAA 
CCATATTTAC ATGGAGGCAT AGCTGTCCAA GTTAAGACTC CTAAAACAGT TTTTTTTGAA 
TCACTGGAGA GGCAGTTAAA ACATCCAAAG TGCCTTATTG TGGATTTTAG CAACCCTGAG 
GCACCTTTAG AGATTCACAC AGCTATGCTT GCCTTGGACC AGTTTCAGGA GAAATACAGT 
CGCAAGCCAA ATGTTGGATG CCAACAAGAT TCAGAAGAAC TGTTGAAACT AGCAACATCT 
ATAAGTGAAA CCTTGGAAGA GAAGCCTGAT GTAAATGCTG ACATTGTGCA TTGGCTCTCT 
TGGACTGCCC AAGGCTTTTT ATCTCCACTT GCTGCAGCAG TAGGAGGTGT TGCCAGCCAA 
GAAGTATTGA AAGCTGTAAC AGGAAAATTT TCTCCTTTGT GCCAGTGGTT ATATCTTGAA 
GCAGCAGATA TTGTTGAATC ACTAGGCAAA CCTGAATGTG AAGAATTTCT CCCACGAGGA 
GATAGATATG ATGCCTTAAG AGCTTGCATT GGAGACACTT TGTGTCAGAA ACTGCAAAAT 
TTAAACATCT TCTTAGTAGG GTGTGGAGCC ATAGGCTGTG AAATGTTGAA AAATTTTGCT 
TTACTTGGTG TTGGCACAAG CAAAGAGAAA GGAATGATTA CAGTTACAGA TCCTGACTTG 
ATAGAGAAAT CCAACTTAAA TAGACAGTTC CTATTTCGTC CTCATCACAT ACAGAAACCT 
AAAAGCTACA CTGCTGCTGA TGCTACTCTG AAAATAAATT CTCAAATAAA GATAGATGCA 
CACCTGAACA AAGTATGTCC AACCACTGAG ACCATTTACA ATGATGAGTT CTATACTAAA 
CAAGATGTAA TTATTACAGC ATTAGATAAT GTGGAAGCCA GGAGATACGT AGACAGTCGT 
TGCTTAGCAA ATCTAAGGCC TCTTTTAGAT TCTGGAACAA TGGGCACTAA GGGACACACT 
GAAGTTATTG TACCGCATTT GACTGAGTCT TACAATAGTC ATCGGGATCC CCCAGAAGAG 
GAAATACCAT TTTGTACTCT AAAATCCTTT CCAGCTGCTA TTGAACATAC CATACAGTGG 
GCAAGAGATA AGTTTGAAAG TTCCTTTTCC CACAAACCTT CATTGTTTAA CAAATTTTGG 
CAAACCTATT CATCTGCAGA AGAAGTCTTA CAGAAGATAC AGAGTGGACA CAGTTTAGAA 
GGCTGTTTTC AAGTTATAAA GTTACTTAGC AGAAGACCTA GAAATTGGTC CCAGTGTGTA 
GAATTAGCAA GATTAAAGTT TGAAAAATAT TTTAACCATA AGGCTCTTCA GCTTCTTCAC 
TGTTTCCCTC TGGACATACG ATTAAAAGAT GGCAGTTTAT TTTGGCAGTC ACCAAAGAGG 
CCACCCTCTC CAATAAAATT TGATTTAAAT GAGCCTTTGC ACCTCAGTTT CCTTCAGAAT 
GCTGCAAAAC TATATGCTAC AGTATATTGT ATTCCATTTG CAGAAGAGGA CTTATCAGCA 
GATGCCCTCT TGAATATTCT TTCAGAAGTA AAGATTCAGG AATTCAAGCC TTCCAATAAG 
GTTGTTCAAA CAGATGAAAC TGCAAGGAAA CCAGACCATG TTCCTATTAG CAGTGAAGAT 
GAGAGGAATG CAATTTTCCA ACTAGAAAAG GCTATTTTAT CTAATGAAGC CACCAAAAGT 
GACCTTCAGA TGGCAGTGCT TTCATTTGAA AAAGATGATG ATCATAATGG ACACATAGAT 
TTCATCACAG CTGCATCAAA TCTTCGTGCC AAAATGTACA GCATTGAACC AGCTGACCGT 
TTCAAAACAA AGCGCATAGC TGGTAAAATT ATACCTGCTA TAGCAACAAC CACTGCTACA 
GTTTCTGGCT TGGTTGCCTT GGAGATGATC AAAGTAACTG GTGGCTATCC ATTTGAAGCT 
TACAAAAATT GTTTTCTTAA CTTAGCCATT CCAATTGTAG TATTTACAGA GACAACTGAA 
GTAAGGAAAA CTAAAATCAG AAATGGAATA TCATTTACAA TTTGGGATCG ATGGACCGTA 
CATGGAAAAG AAGATTTCAC CCTCTTGGAT TTCATAAATG CAGTCAAAGA GAAGTATGGA 
ATTGAGCCAA CAATGGTGGT ACAGGGAGTC AAAATGCTTT ATGTTCCTGT AATGCCTGGT 
CATGCAAAAA GATTGAAGTT AACAATGCAT AAACTTGTAA AACCTACTAC TGAAAAGAAA 
TATGTGGATC TTACTGTGTC ATTTGCTCCA GACATTGATG GAGATGAAGA TTTGCCGGGA 
CCTCCAGTAA GATACTACTT CAGTCATGAC ACTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060697.4
CDS	53..3211
Translation	MEGSEPVAAHQGEEASCSSWGTGSTNKNLPIMSTASVEIDDALYSRQRYVLGDTAMQKMAKSHVFLSGMGGLGLEIAKNLVLAGIKAVTIHDTEKCQAWDLGTNFFLSEDDVVNKRNRAEAVLKHIAELNPYVHVTSSSVPFNETTDLSFLDKYQCVVLTEMKLPLQKKINDFCRSQCPPIKFISADVHGIWSRLFCDFGDEFEVLDTTGEEPKEIFISNITQANPGIVTCLENHPHKLETGQFLTFREINGMTGLNGSIQQITVISPFSFSIGDTTELEPYLHGGIAVQVKTPKTVFFESLERQLKHPKCLIVDFSNPEAPLEIHTAMLALDQFQEKYSRKPNVGCQQDSEELLKLATSISETLEEKPDVNADIVHWLSWTAQGFLSPLAAAVGGVASQEVLKAVTGKFSPLCQWLYLEAADIVESLGKPECEEFLPRGDRYDALRACIGDTLCQKLQNLNIFLVGCGAIGCEMLKNFALLGVGTSKEKGMITVTDPDLIEKSNLNRQFLFRPHHIQKPKSYTAADATLKINSQIKIDAHLNKVCPTTETIYNDEFYTKQDVIITALDNVEARRYVDSRCLANLRPLLDSGTMGTKGHTEVIVPHLTESYNSHRDPPEEEIPFCTLKSFPAAIEHTIQWARDKFESSFSHKPSLFNKFWQTYSSAEEVLQKIQSGHSLEGCFQVIKLLSRRPRNWSQCVELARLKFEKYFNHKALQLLHCFPLDIRLKDGSLFWQSPKRPPSPIKFDLNEPLHLSFLQNAAKLYATVYCIPFAEEDLSADALLNILSEVKIQEFKPSNKVVQTDETARKPDHVPISSEDERNAIFQLEKAILSNEATKSDLQMAVLSFEKDDDHNGHIDFITAASNLRAKMYSIEPADRFKTKRIAGKIIPAIATTTATVSGLVALEMIKVTGGYPFEAYKNCFLNLAIPIVVFTETTEVRKTKIRNGISFTIWDRWTVHGKEDFTLLDFINAVKEKYGIEPTMVVQGVKMLYVPVMPGHAKRLKLTMHKLVKPTTEKKYVDLTVSFAPDIDGDEDLPGPPVRYYFSHDTD

Target ORF information:

RefSeq Version	NM_018227.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin like modifier activating enzyme 6 (UBA6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018227.5

ORF Insert Sequence:

ATGGAAGGAT CCGAGCCTGT GGCCGCCCAT CAGGGGGAAG AGGCGTCCTG TTCTTCCTGG 
GGGACTGGCA GCACAAATAA AAATTTGCCC ATTATGTCAA CAGCATCTGT GGAAATCGAT 
GATGCATTGT ATAGTCGACA GAGGTACGTT CTTGGAGACA CAGCAATGCA GAAGATGGCC 
AAGTCCCATG TTTTCTTAAG TGGGATGGGT GGTCTTGGTT TGGAAATTGC AAAGAATCTT 
GTTCTTGCAG GGATTAAGGC AGTTACAATT CATGATACAG AAAAATGCCA AGCATGGGAT 
CTAGGAACCA ACTTCTTTCT CAGTGAAGAT GATGTTGTTA ATAAGAGAAA CAGGGCTGAA 
GCTGTACTTA AACATATTGC AGAACTAAAT CCATACGTTC ATGTCACATC ATCTTCTGTT 
CCTTTCAATG AGACCACAGA TCTCTCCTTT TTAGATAAAT ACCAGTGTGT AGTATTGACT 
GAGATGAAAC TTCCATTGCA GAAGAAGATC AATGACTTTT GCCGTTCTCA GTGCCCTCCA 
ATTAAGTTTA TCAGTGCAGA TGTACATGGA ATTTGGTCAA GGTTATTTTG TGATTTCGGT 
GATGAATTTG AAGTTTTAGA TACAACAGGA GAAGAACCAA AAGAAATTTT CATTTCAAAC 
ATAACGCAAG CAAATCCTGG CATTGTTACT TGCCTTGAAA ATCATCCTCA CAAACTGGAG 
ACAGGACAAT TCCTAACATT TCGAGAAATT AATGGAATGA CAGGTTTAAA TGGATCTATA 
CAACAAATAA CGGTGATATC GCCATTTTCT TTTAGTATTG GTGACACCAC AGAACTGGAA 
CCATATTTAC ATGGAGGCAT AGCTGTCCAA GTTAAGACTC CTAAAACAGT TTTTTTTGAA 
TCACTGGAGA GGCAGTTAAA ACATCCAAAG TGCCTTATTG TGGATTTTAG CAACCCTGAG 
GCACCTTTAG AGATTCACAC AGCTATGCTT GCCTTGGACC AGTTTCAGGA GAAATACAGT 
CGCAAGCCAA ATGTTGGATG CCAACAAGAT TCAGAAGAAC TGTTGAAACT AGCAACATCT 
ATAAGTGAAA CCTTGGAAGA GAAGCCTGAT GTAAATGCTG ACATTGTGCA TTGGCTCTCT 
TGGACTGCCC AAGGCTTTTT ATCTCCACTT GCTGCAGCAG TAGGAGGTGT TGCCAGCCAA 
GAAGTATTGA AAGCTGTAAC AGGAAAATTT TCTCCTTTGT GCCAGTGGTT ATATCTTGAA 
GCAGCAGATA TTGTTGAATC ACTAGGCAAA CCTGAATGTG AAGAATTTCT CCCACGAGGA 
GATAGATATG ATGCCTTAAG AGCTTGCATT GGAGACACTT TGTGTCAGAA ACTGCAAAAT 
TTAAACATCT TCTTAGTAGG GTGTGGAGCC ATAGGCTGTG AAATGTTGAA AAATTTTGCT 
TTACTTGGTG TTGGCACAAG CAAAGAGAAA GGAATGATTA CAGTTACAGA TCCTGACTTG 
ATAGAGAAAT CCAACTTAAA TAGACAGTTC CTATTTCGTC CTCATCACAT ACAGAAACCT 
AAAAGCTACA CTGCTGCTGA TGCTACTCTG AAAATAAATT CTCAAATAAA GATAGATGCA 
CACCTGAACA AAGTATGTCC AACCACTGAG ACCATTTACA ATGATGAGTT CTATACTAAA 
CAAGATGTAA TTATTACAGC ATTAGATAAT GTGGAAGCCA GGAGATACGT AGACAGTCGT 
TGCTTAGCAA ATCTAAGGCC TCTTTTAGAT TCTGGAACAA TGGGCACTAA GGGACACACT 
GAAGTTATTG TACCGCATTT GACTGAGTCT TACAATAGTC ATCGGGATCC CCCAGAAGAG 
GAAATACCAT TTTGTACTCT AAAATCCTTT CCAGCTGCTA TTGAACATAC CATACAGTGG 
GCAAGAGATA AGTTTGAAAG TTCCTTTTCC CACAAACCTT CATTGTTTAA CAAATTTTGG 
CAAACCTATT CATCTGCAGA AGAAGTCTTA CAGAAGATAC AGAGTGGACA CAGTTTAGAA 
GGCTGTTTTC AAGTTATAAA GTTACTTAGC AGAAGACCTA GAAATTGGTC CCAGTGTGTA 
GAATTAGCAA GATTAAAGTT TGAAAAATAT TTTAACCATA AGGCTCTTCA GCTTCTTCAC 
TGTTTCCCTC TGGACATACG ATTAAAAGAT GGCAGTTTAT TTTGGCAGTC ACCAAAGAGG 
CCACCCTCTC CAATAAAATT TGATTTAAAT GAGCCTTTGC ACCTCAGTTT CCTTCAGAAT 
GCTGCAAAAC TATATGCTAC AGTATATTGT ATTCCATTTG CAGAAGAGGA CTTATCAGCA 
GATGCCCTCT TGAATATTCT TTCAGAAGTA AAGATTCAGG AATTCAAGCC TTCCAATAAG 
GTTGTTCAAA CAGATGAAAC TGCAAGGAAA CCAGACCATG TTCCTATTAG CAGTGAAGAT 
GAGAGGAATG CAATTTTCCA ACTAGAAAAG GCTATTTTAT CTAATGAAGC CACCAAAAGT 
GACCTTCAGA TGGCAGTGCT TTCATTTGAA AAAGATGATG ATCATAATGG ACACATAGAT 
TTCATCACAG CTGCATCAAA TCTTCGTGCC AAAATGTACA GCATTGAACC AGCTGACCGT 
TTCAAAACAA AGCGCATAGC TGGTAAAATT ATACCTGCTA TAGCAACAAC CACTGCTACA 
GTTTCTGGCT TGGTTGCCTT GGAGATGATC AAAGTAACTG GTGGCTATCC ATTTGAAGCT 
TACAAAAATT GTTTTCTTAA CTTAGCCATT CCAATTGTAG TATTTACAGA GACAACTGAA 
GTAAGGAAAA CTAAAATCAG AAATGGAATA TCATTTACAA TTTGGGATCG ATGGACCGTA 
CATGGAAAAG AAGATTTCAC CCTCTTGGAT TTCATAAATG CAGTCAAAGA GAAGTATGGA 
ATTGAGCCAA CAATGGTGGT ACAGGGAGTC AAAATGCTTT ATGTTCCTGT AATGCCTGGT 
CATGCAAAAA GATTGAAGTT AACAATGCAT AAACTTGTAA AACCTACTAC TGAAAAGAAA 
TATGTGGATC TTACTGTGTC ATTTGCTCCA GACATTGATG GAGATGAAGA TTTGCCGGGA 
CCTCCAGTAA GATACTACTT CAGTCATGAC ACTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBA6 ( XM_017008359.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863848.1 Homo sapiens ubiquitin like modifier activating enzyme 6 (UBA6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu95274
Clone ID Related Accession (Same CDS sequence)	XM_017008359.1 , XM_017008359.2
Accession Version	XM_017008359.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ubiquitin-like modifier-activating enzyme 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGAT CCGAGCCTGT GGCCGCCCAT CAGGGGGAAG AGGCGTCCTG TTCTTCCTGG 
GGGACTGGCA GCACAAATAA AAATTTGCCC ATTATGTCAA CAGCATCTGT GGAAATCGAT 
GATGCATTGT ATAGTCGACA GAGGTACGTT CTTGGAGACA CAGCAATGCA GAAGATGGCC 
AAGTCCCATG TTTTCTTAAG TGGGATGGGT GGTCTTGGTT TGGAAATTGC AAAGAATCTT 
GTTCTTGCAG GGATTAAGGC AGTTACAATT CATGATACAG AAAAATGCCA AGCATGGGAT 
CTAGGAACCA ACTTCTTTCT CAGTGAAGAT GATGTTGTTA ATAAGAGAAA CAGGGCTGAA 
GCTGTACTTA AACATATTGC AGAACTAAAT CCATACGTTC ATGTCACATC ATCTTCTGTT 
CCTTTCAATG AGACCACAGA TCTCTCCTTT TTAGATAAAT ACCAGTGTGT AGTATTGACT 
GAGATGAAAC TTCCATTGCA GAAGAAGATC AATGACTTTT GCCGTTCTCA GTGCCCTCCA 
ATTAAGTTTA TCAGTGCAGA TGTACATGGA ATTTGGTCAA GGTTATTTTG TGATTTCGGT 
GATGAATTTG AAGTTTTAGA TACAACAGGA GAAGAACCAA AAGAAATTTT CATTTCAAAC 
ATAACGCAAG CAAATCCTGG CATTGTTACT TGCCTTGAAA ATCATCCTCA CAAACTGGAG 
ACAGGACAAT TCCTAACATT TCGAGAAATT AATGGAATGA CAGGTTTAAA TGGATCTATA 
CAACAAATAA CGGTGATATC GCCATTTTCT TTTAGTATTG GTGACACCAC AGAACTGGAA 
CCATATTTAC ATGGAGGCAT AGCTGTCCAA GTTAAGACTC CTAAAACAGT TTTTTTTGAA 
TCACTGGAGA GGCAGTTAAA ACATCCAAAG TGCCTTATTG TGGATTTTAG CAACCCTGAG 
GCACCTTTAG AGATTCACAC AGCTATGCTT GCCTTGGACC AGTTTCAGGA GAAATACAGT 
CGCAAGCCAA ATGTTGGATG CCAACAAGAT TCAGAAGAAC TGTTGAAACT AGCAACATCT 
ATAAGTGAAA CCTTGGAAGA GAAGCCTGAT GTAAATGCTG ACATTGTGCA TTGGCTCTCT 
TGGACTGCCC AAGGCTTTTT ATCTCCACTT GCTGCAGCAG TAGGAGGTGT TGCCAGCCAA 
GAAGTATTGA AAGCTGTAAC AGGAAAATTT TCTCCTTTGT GCCAGTGGTT ATATCTTGAA 
GCAGCAGATA TTGTTGAATC ACTAGGCAAA CCTGAATGTG AAGAATTTCT CCCACGAGGA 
GATAGATATG ATGCCTTAAG AGCTTGCATT GGAGACACTT TGTGTCAGAA ACTGCAAAAT 
TTAAACATCT TCTTAGTAGG GTGTGGAGCC ATAGGCTGTG AAATGTTGAA AAATTTTGCT 
TTACTTGGTG TTGGCACAAG CAAAGAGAAA GGAATGATTA CAGTTACAGA TCCTGACTTG 
ATAGAGAAAT CCAACTTAAA TAGACAGTTC CTATTTCGTC CTCATCACAT ACAGAAACCT 
AAAAGCTACA CTGCTGCTGA TGCTACTCTG AAAATAAATT CTCAAATAAA GATAGATGCA 
CACCTGAACA AAGTATGTCC AACCACTGAG ACCATTTACA ATGATGAGTT CTATACTAAA 
CAAGATGTAA TTATTACAGC ATTAGATAAT GTGGAAGCCA GGAGATACGT AGACAGTCGT 
TGCTTAGCAA ATCTAAGGCC TCTTTTAGAT TCTGGAACAA TGGGCACTAA GGGACACACT 
GAAGTTATTG TACCGCATTT GACTGAGTCT TACAATAGTC ATCGGGATCC CCCAGAAGAG 
GAAATACCAT TTTGTACTCT AAAATCCTTT CCAGCTGCTA TTGAACATAC CATACAGTGG 
GCAAGAGATA AGTTTGAAAG TTCCTTTTCC CACAAACCTT CATTGTTTAA CAAATTTTGG 
CAAACCTATT CATCTGCAGA AGAAGTCTTA CAGAAGATAC AGAGTGGACA CAGTTTAGAA 
GGCTGTTTTC AAGTTATAAA GTTACTTAGC AGAAGACCTA GAAATTGGTC CCAGTGTGTA 
GAATTAGCAA GATTAAAGTT TGAAAAATAT TTTAACCATA AGGCTCTTCA GCTTCTTCAC 
TGTTTCCCTC TGGACATACG ATTAAAAGAT GGCAGTTTAT TTTGGCAGTC ACCAAAGAGG 
CCACCCTCTC CAATAAAATT TGATTTAAAT GAGCCTTTGC ACCTCAGTTT CCTTCAGAAT 
GCTGCAAAAC TATATGCTAC AGTATATTGT ATTCCATTTG CAGAAGAGGA CTTATCAGCA 
GATGCCCTCT TGAATATTCT TTCAGAAGTA AAGATTCAGG AATTCAAGCC TTCCAATAAG 
GTTGTTCAAA CAGATGAAAC TGCAAGGAAA CCAGACCATG TTCCTATTAG CAGTGAAGAT 
GAGAGGAATG CAATTTTCCA ACTAGAAAAG GCTATTTTAT CTAATGAAGC CACCAAAAGT 
GACCTTCAGA TGGCAGTGCT TTCATTTGAA AAAGATGATG ATCATAATGG ACACATAGAT 
TTCATCACAG CTGCATCAAA TCTTCGTGCC AAAATGTACA GCATTGAACC AGCTGACCGT 
TTCAAAACAA AGCGCATAGC TGGTAAAATT ATACCTGCTA TAGCAACAAC CACTGCTACA 
GTTTCTGGCT TGGTTGCCTT GGAGATGATC AAAGTAACTG GTGGCTATCC ATTTGAAGCT 
TACAAAAATT GTTTTCTTAA CTTAGCCATT CCAATTGTAG TATTTACAGA GACAACTGAA 
GTAAGGAAAA CTAAAATCAG AAATGGAATA TCATTTACAA TTTGGGATCG ATGGACCGTA 
CATGGAAAAG AAGATTTCAC CCTCTTGGAT TTCATAAATG CAGTCAAAGA GAAGTATGGA 
ATTGAGCCAA CAATGGTGGT ACAGGGAGTC AAAATGCTTT ATGTTCCTGT AATGCCTGGT 
CATGCAAAAA GATTGAAGTT AACACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863848.1
CDS	30..3059
Translation	MEGSEPVAAHQGEEASCSSWGTGSTNKNLPIMSTASVEIDDALYSRQRYVLGDTAMQKMAKSHVFLSGMGGLGLEIAKNLVLAGIKAVTIHDTEKCQAWDLGTNFFLSEDDVVNKRNRAEAVLKHIAELNPYVHVTSSSVPFNETTDLSFLDKYQCVVLTEMKLPLQKKINDFCRSQCPPIKFISADVHGIWSRLFCDFGDEFEVLDTTGEEPKEIFISNITQANPGIVTCLENHPHKLETGQFLTFREINGMTGLNGSIQQITVISPFSFSIGDTTELEPYLHGGIAVQVKTPKTVFFESLERQLKHPKCLIVDFSNPEAPLEIHTAMLALDQFQEKYSRKPNVGCQQDSEELLKLATSISETLEEKPDVNADIVHWLSWTAQGFLSPLAAAVGGVASQEVLKAVTGKFSPLCQWLYLEAADIVESLGKPECEEFLPRGDRYDALRACIGDTLCQKLQNLNIFLVGCGAIGCEMLKNFALLGVGTSKEKGMITVTDPDLIEKSNLNRQFLFRPHHIQKPKSYTAADATLKINSQIKIDAHLNKVCPTTETIYNDEFYTKQDVIITALDNVEARRYVDSRCLANLRPLLDSGTMGTKGHTEVIVPHLTESYNSHRDPPEEEIPFCTLKSFPAAIEHTIQWARDKFESSFSHKPSLFNKFWQTYSSAEEVLQKIQSGHSLEGCFQVIKLLSRRPRNWSQCVELARLKFEKYFNHKALQLLHCFPLDIRLKDGSLFWQSPKRPPSPIKFDLNEPLHLSFLQNAAKLYATVYCIPFAEEDLSADALLNILSEVKIQEFKPSNKVVQTDETARKPDHVPISSEDERNAIFQLEKAILSNEATKSDLQMAVLSFEKDDDHNGHIDFITAASNLRAKMYSIEPADRFKTKRIAGKIIPAIATTTATVSGLVALEMIKVTGGYPFEAYKNCFLNLAIPIVVFTETTEVRKTKIRNGISFTIWDRWTVHGKEDFTLLDFINAVKEKYGIEPTMVVQGVKMLYVPVMPGHAKRLKLTH

Target ORF information:

RefSeq Version	XM_017008359.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin like modifier activating enzyme 6 (UBA6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008359.1

ORF Insert Sequence:

ATGGAAGGAT CCGAGCCTGT GGCCGCCCAT CAGGGGGAAG AGGCGTCCTG TTCTTCCTGG 
GGGACTGGCA GCACAAATAA AAATTTGCCC ATTATGTCAA CAGCATCTGT GGAAATCGAT 
GATGCATTGT ATAGTCGACA GAGGTACGTT CTTGGAGACA CAGCAATGCA GAAGATGGCC 
AAGTCCCATG TTTTCTTAAG TGGGATGGGT GGTCTTGGTT TGGAAATTGC AAAGAATCTT 
GTTCTTGCAG GGATTAAGGC AGTTACAATT CATGATACAG AAAAATGCCA AGCATGGGAT 
CTAGGAACCA ACTTCTTTCT CAGTGAAGAT GATGTTGTTA ATAAGAGAAA CAGGGCTGAA 
GCTGTACTTA AACATATTGC AGAACTAAAT CCATACGTTC ATGTCACATC ATCTTCTGTT 
CCTTTCAATG AGACCACAGA TCTCTCCTTT TTAGATAAAT ACCAGTGTGT AGTATTGACT 
GAGATGAAAC TTCCATTGCA GAAGAAGATC AATGACTTTT GCCGTTCTCA GTGCCCTCCA 
ATTAAGTTTA TCAGTGCAGA TGTACATGGA ATTTGGTCAA GGTTATTTTG TGATTTCGGT 
GATGAATTTG AAGTTTTAGA TACAACAGGA GAAGAACCAA AAGAAATTTT CATTTCAAAC 
ATAACGCAAG CAAATCCTGG CATTGTTACT TGCCTTGAAA ATCATCCTCA CAAACTGGAG 
ACAGGACAAT TCCTAACATT TCGAGAAATT AATGGAATGA CAGGTTTAAA TGGATCTATA 
CAACAAATAA CGGTGATATC GCCATTTTCT TTTAGTATTG GTGACACCAC AGAACTGGAA 
CCATATTTAC ATGGAGGCAT AGCTGTCCAA GTTAAGACTC CTAAAACAGT TTTTTTTGAA 
TCACTGGAGA GGCAGTTAAA ACATCCAAAG TGCCTTATTG TGGATTTTAG CAACCCTGAG 
GCACCTTTAG AGATTCACAC AGCTATGCTT GCCTTGGACC AGTTTCAGGA GAAATACAGT 
CGCAAGCCAA ATGTTGGATG CCAACAAGAT TCAGAAGAAC TGTTGAAACT AGCAACATCT 
ATAAGTGAAA CCTTGGAAGA GAAGCCTGAT GTAAATGCTG ACATTGTGCA TTGGCTCTCT 
TGGACTGCCC AAGGCTTTTT ATCTCCACTT GCTGCAGCAG TAGGAGGTGT TGCCAGCCAA 
GAAGTATTGA AAGCTGTAAC AGGAAAATTT TCTCCTTTGT GCCAGTGGTT ATATCTTGAA 
GCAGCAGATA TTGTTGAATC ACTAGGCAAA CCTGAATGTG AAGAATTTCT CCCACGAGGA 
GATAGATATG ATGCCTTAAG AGCTTGCATT GGAGACACTT TGTGTCAGAA ACTGCAAAAT 
TTAAACATCT TCTTAGTAGG GTGTGGAGCC ATAGGCTGTG AAATGTTGAA AAATTTTGCT 
TTACTTGGTG TTGGCACAAG CAAAGAGAAA GGAATGATTA CAGTTACAGA TCCTGACTTG 
ATAGAGAAAT CCAACTTAAA TAGACAGTTC CTATTTCGTC CTCATCACAT ACAGAAACCT 
AAAAGCTACA CTGCTGCTGA TGCTACTCTG AAAATAAATT CTCAAATAAA GATAGATGCA 
CACCTGAACA AAGTATGTCC AACCACTGAG ACCATTTACA ATGATGAGTT CTATACTAAA 
CAAGATGTAA TTATTACAGC ATTAGATAAT GTGGAAGCCA GGAGATACGT AGACAGTCGT 
TGCTTAGCAA ATCTAAGGCC TCTTTTAGAT TCTGGAACAA TGGGCACTAA GGGACACACT 
GAAGTTATTG TACCGCATTT GACTGAGTCT TACAATAGTC ATCGGGATCC CCCAGAAGAG 
GAAATACCAT TTTGTACTCT AAAATCCTTT CCAGCTGCTA TTGAACATAC CATACAGTGG 
GCAAGAGATA AGTTTGAAAG TTCCTTTTCC CACAAACCTT CATTGTTTAA CAAATTTTGG 
CAAACCTATT CATCTGCAGA AGAAGTCTTA CAGAAGATAC AGAGTGGACA CAGTTTAGAA 
GGCTGTTTTC AAGTTATAAA GTTACTTAGC AGAAGACCTA GAAATTGGTC CCAGTGTGTA 
GAATTAGCAA GATTAAAGTT TGAAAAATAT TTTAACCATA AGGCTCTTCA GCTTCTTCAC 
TGTTTCCCTC TGGACATACG ATTAAAAGAT GGCAGTTTAT TTTGGCAGTC ACCAAAGAGG 
CCACCCTCTC CAATAAAATT TGATTTAAAT GAGCCTTTGC ACCTCAGTTT CCTTCAGAAT 
GCTGCAAAAC TATATGCTAC AGTATATTGT ATTCCATTTG CAGAAGAGGA CTTATCAGCA 
GATGCCCTCT TGAATATTCT TTCAGAAGTA AAGATTCAGG AATTCAAGCC TTCCAATAAG 
GTTGTTCAAA CAGATGAAAC TGCAAGGAAA CCAGACCATG TTCCTATTAG CAGTGAAGAT 
GAGAGGAATG CAATTTTCCA ACTAGAAAAG GCTATTTTAT CTAATGAAGC CACCAAAAGT 
GACCTTCAGA TGGCAGTGCT TTCATTTGAA AAAGATGATG ATCATAATGG ACACATAGAT 
TTCATCACAG CTGCATCAAA TCTTCGTGCC AAAATGTACA GCATTGAACC AGCTGACCGT 
TTCAAAACAA AGCGCATAGC TGGTAAAATT ATACCTGCTA TAGCAACAAC CACTGCTACA 
GTTTCTGGCT TGGTTGCCTT GGAGATGATC AAAGTAACTG GTGGCTATCC ATTTGAAGCT 
TACAAAAATT GTTTTCTTAA CTTAGCCATT CCAATTGTAG TATTTACAGA GACAACTGAA 
GTAAGGAAAA CTAAAATCAG AAATGGAATA TCATTTACAA TTTGGGATCG ATGGACCGTA 
CATGGAAAAG AAGATTTCAC CCTCTTGGAT TTCATAAATG CAGTCAAAGA GAAGTATGGA 
ATTGAGCCAA CAATGGTGGT ACAGGGAGTC AAAATGCTTT ATGTTCCTGT AATGCCTGGT 
CATGCAAAAA GATTGAAGTT AACACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBA6 ( XM_017008359.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863848.1 Homo sapiens ubiquitin like modifier activating enzyme 6 (UBA6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu95274
Clone ID Related Accession (Same CDS sequence)	XM_017008359.1 , XM_017008359.2
Accession Version	XM_017008359.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ubiquitin-like modifier-activating enzyme 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017008359.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGAT CCGAGCCTGT GGCCGCCCAT CAGGGGGAAG AGGCGTCCTG TTCTTCCTGG 
GGGACTGGCA GCACAAATAA AAATTTGCCC ATTATGTCAA CAGCATCTGT GGAAATCGAT 
GATGCATTGT ATAGTCGACA GAGGTACGTT CTTGGAGACA CAGCAATGCA GAAGATGGCC 
AAGTCCCATG TTTTCTTAAG TGGGATGGGT GGTCTTGGTT TGGAAATTGC AAAGAATCTT 
GTTCTTGCAG GGATTAAGGC AGTTACAATT CATGATACAG AAAAATGCCA AGCATGGGAT 
CTAGGAACCA ACTTCTTTCT CAGTGAAGAT GATGTTGTTA ATAAGAGAAA CAGGGCTGAA 
GCTGTACTTA AACATATTGC AGAACTAAAT CCATACGTTC ATGTCACATC ATCTTCTGTT 
CCTTTCAATG AGACCACAGA TCTCTCCTTT TTAGATAAAT ACCAGTGTGT AGTATTGACT 
GAGATGAAAC TTCCATTGCA GAAGAAGATC AATGACTTTT GCCGTTCTCA GTGCCCTCCA 
ATTAAGTTTA TCAGTGCAGA TGTACATGGA ATTTGGTCAA GGTTATTTTG TGATTTCGGT 
GATGAATTTG AAGTTTTAGA TACAACAGGA GAAGAACCAA AAGAAATTTT CATTTCAAAC 
ATAACGCAAG CAAATCCTGG CATTGTTACT TGCCTTGAAA ATCATCCTCA CAAACTGGAG 
ACAGGACAAT TCCTAACATT TCGAGAAATT AATGGAATGA CAGGTTTAAA TGGATCTATA 
CAACAAATAA CGGTGATATC GCCATTTTCT TTTAGTATTG GTGACACCAC AGAACTGGAA 
CCATATTTAC ATGGAGGCAT AGCTGTCCAA GTTAAGACTC CTAAAACAGT TTTTTTTGAA 
TCACTGGAGA GGCAGTTAAA ACATCCAAAG TGCCTTATTG TGGATTTTAG CAACCCTGAG 
GCACCTTTAG AGATTCACAC AGCTATGCTT GCCTTGGACC AGTTTCAGGA GAAATACAGT 
CGCAAGCCAA ATGTTGGATG CCAACAAGAT TCAGAAGAAC TGTTGAAACT AGCAACATCT 
ATAAGTGAAA CCTTGGAAGA GAAGCCTGAT GTAAATGCTG ACATTGTGCA TTGGCTCTCT 
TGGACTGCCC AAGGCTTTTT ATCTCCACTT GCTGCAGCAG TAGGAGGTGT TGCCAGCCAA 
GAAGTATTGA AAGCTGTAAC AGGAAAATTT TCTCCTTTGT GCCAGTGGTT ATATCTTGAA 
GCAGCAGATA TTGTTGAATC ACTAGGCAAA CCTGAATGTG AAGAATTTCT CCCACGAGGA 
GATAGATATG ATGCCTTAAG AGCTTGCATT GGAGACACTT TGTGTCAGAA ACTGCAAAAT 
TTAAACATCT TCTTAGTAGG GTGTGGAGCC ATAGGCTGTG AAATGTTGAA AAATTTTGCT 
TTACTTGGTG TTGGCACAAG CAAAGAGAAA GGAATGATTA CAGTTACAGA TCCTGACTTG 
ATAGAGAAAT CCAACTTAAA TAGACAGTTC CTATTTCGTC CTCATCACAT ACAGAAACCT 
AAAAGCTACA CTGCTGCTGA TGCTACTCTG AAAATAAATT CTCAAATAAA GATAGATGCA 
CACCTGAACA AAGTATGTCC AACCACTGAG ACCATTTACA ATGATGAGTT CTATACTAAA 
CAAGATGTAA TTATTACAGC ATTAGATAAT GTGGAAGCCA GGAGATACGT AGACAGTCGT 
TGCTTAGCAA ATCTAAGGCC TCTTTTAGAT TCTGGAACAA TGGGCACTAA GGGACACACT 
GAAGTTATTG TACCGCATTT GACTGAGTCT TACAATAGTC ATCGGGATCC CCCAGAAGAG 
GAAATACCAT TTTGTACTCT AAAATCCTTT CCAGCTGCTA TTGAACATAC CATACAGTGG 
GCAAGAGATA AGTTTGAAAG TTCCTTTTCC CACAAACCTT CATTGTTTAA CAAATTTTGG 
CAAACCTATT CATCTGCAGA AGAAGTCTTA CAGAAGATAC AGAGTGGACA CAGTTTAGAA 
GGCTGTTTTC AAGTTATAAA GTTACTTAGC AGAAGACCTA GAAATTGGTC CCAGTGTGTA 
GAATTAGCAA GATTAAAGTT TGAAAAATAT TTTAACCATA AGGCTCTTCA GCTTCTTCAC 
TGTTTCCCTC TGGACATACG ATTAAAAGAT GGCAGTTTAT TTTGGCAGTC ACCAAAGAGG 
CCACCCTCTC CAATAAAATT TGATTTAAAT GAGCCTTTGC ACCTCAGTTT CCTTCAGAAT 
GCTGCAAAAC TATATGCTAC AGTATATTGT ATTCCATTTG CAGAAGAGGA CTTATCAGCA 
GATGCCCTCT TGAATATTCT TTCAGAAGTA AAGATTCAGG AATTCAAGCC TTCCAATAAG 
GTTGTTCAAA CAGATGAAAC TGCAAGGAAA CCAGACCATG TTCCTATTAG CAGTGAAGAT 
GAGAGGAATG CAATTTTCCA ACTAGAAAAG GCTATTTTAT CTAATGAAGC CACCAAAAGT 
GACCTTCAGA TGGCAGTGCT TTCATTTGAA AAAGATGATG ATCATAATGG ACACATAGAT 
TTCATCACAG CTGCATCAAA TCTTCGTGCC AAAATGTACA GCATTGAACC AGCTGACCGT 
TTCAAAACAA AGCGCATAGC TGGTAAAATT ATACCTGCTA TAGCAACAAC CACTGCTACA 
GTTTCTGGCT TGGTTGCCTT GGAGATGATC AAAGTAACTG GTGGCTATCC ATTTGAAGCT 
TACAAAAATT GTTTTCTTAA CTTAGCCATT CCAATTGTAG TATTTACAGA GACAACTGAA 
GTAAGGAAAA CTAAAATCAG AAATGGAATA TCATTTACAA TTTGGGATCG ATGGACCGTA 
CATGGAAAAG AAGATTTCAC CCTCTTGGAT TTCATAAATG CAGTCAAAGA GAAGTATGGA 
ATTGAGCCAA CAATGGTGGT ACAGGGAGTC AAAATGCTTT ATGTTCCTGT AATGCCTGGT 
CATGCAAAAA GATTGAAGTT AACACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863848.1
CDS	37..3066
Translation	MEGSEPVAAHQGEEASCSSWGTGSTNKNLPIMSTASVEIDDALYSRQRYVLGDTAMQKMAKSHVFLSGMGGLGLEIAKNLVLAGIKAVTIHDTEKCQAWDLGTNFFLSEDDVVNKRNRAEAVLKHIAELNPYVHVTSSSVPFNETTDLSFLDKYQCVVLTEMKLPLQKKINDFCRSQCPPIKFISADVHGIWSRLFCDFGDEFEVLDTTGEEPKEIFISNITQANPGIVTCLENHPHKLETGQFLTFREINGMTGLNGSIQQITVISPFSFSIGDTTELEPYLHGGIAVQVKTPKTVFFESLERQLKHPKCLIVDFSNPEAPLEIHTAMLALDQFQEKYSRKPNVGCQQDSEELLKLATSISETLEEKPDVNADIVHWLSWTAQGFLSPLAAAVGGVASQEVLKAVTGKFSPLCQWLYLEAADIVESLGKPECEEFLPRGDRYDALRACIGDTLCQKLQNLNIFLVGCGAIGCEMLKNFALLGVGTSKEKGMITVTDPDLIEKSNLNRQFLFRPHHIQKPKSYTAADATLKINSQIKIDAHLNKVCPTTETIYNDEFYTKQDVIITALDNVEARRYVDSRCLANLRPLLDSGTMGTKGHTEVIVPHLTESYNSHRDPPEEEIPFCTLKSFPAAIEHTIQWARDKFESSFSHKPSLFNKFWQTYSSAEEVLQKIQSGHSLEGCFQVIKLLSRRPRNWSQCVELARLKFEKYFNHKALQLLHCFPLDIRLKDGSLFWQSPKRPPSPIKFDLNEPLHLSFLQNAAKLYATVYCIPFAEEDLSADALLNILSEVKIQEFKPSNKVVQTDETARKPDHVPISSEDERNAIFQLEKAILSNEATKSDLQMAVLSFEKDDDHNGHIDFITAASNLRAKMYSIEPADRFKTKRIAGKIIPAIATTTATVSGLVALEMIKVTGGYPFEAYKNCFLNLAIPIVVFTETTEVRKTKIRNGISFTIWDRWTVHGKEDFTLLDFINAVKEKYGIEPTMVVQGVKMLYVPVMPGHAKRLKLTH

Target ORF information:

RefSeq Version	XM_017008359.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin like modifier activating enzyme 6 (UBA6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008359.2

ORF Insert Sequence:

ATGGAAGGAT CCGAGCCTGT GGCCGCCCAT CAGGGGGAAG AGGCGTCCTG TTCTTCCTGG 
GGGACTGGCA GCACAAATAA AAATTTGCCC ATTATGTCAA CAGCATCTGT GGAAATCGAT 
GATGCATTGT ATAGTCGACA GAGGTACGTT CTTGGAGACA CAGCAATGCA GAAGATGGCC 
AAGTCCCATG TTTTCTTAAG TGGGATGGGT GGTCTTGGTT TGGAAATTGC AAAGAATCTT 
GTTCTTGCAG GGATTAAGGC AGTTACAATT CATGATACAG AAAAATGCCA AGCATGGGAT 
CTAGGAACCA ACTTCTTTCT CAGTGAAGAT GATGTTGTTA ATAAGAGAAA CAGGGCTGAA 
GCTGTACTTA AACATATTGC AGAACTAAAT CCATACGTTC ATGTCACATC ATCTTCTGTT 
CCTTTCAATG AGACCACAGA TCTCTCCTTT TTAGATAAAT ACCAGTGTGT AGTATTGACT 
GAGATGAAAC TTCCATTGCA GAAGAAGATC AATGACTTTT GCCGTTCTCA GTGCCCTCCA 
ATTAAGTTTA TCAGTGCAGA TGTACATGGA ATTTGGTCAA GGTTATTTTG TGATTTCGGT 
GATGAATTTG AAGTTTTAGA TACAACAGGA GAAGAACCAA AAGAAATTTT CATTTCAAAC 
ATAACGCAAG CAAATCCTGG CATTGTTACT TGCCTTGAAA ATCATCCTCA CAAACTGGAG 
ACAGGACAAT TCCTAACATT TCGAGAAATT AATGGAATGA CAGGTTTAAA TGGATCTATA 
CAACAAATAA CGGTGATATC GCCATTTTCT TTTAGTATTG GTGACACCAC AGAACTGGAA 
CCATATTTAC ATGGAGGCAT AGCTGTCCAA GTTAAGACTC CTAAAACAGT TTTTTTTGAA 
TCACTGGAGA GGCAGTTAAA ACATCCAAAG TGCCTTATTG TGGATTTTAG CAACCCTGAG 
GCACCTTTAG AGATTCACAC AGCTATGCTT GCCTTGGACC AGTTTCAGGA GAAATACAGT 
CGCAAGCCAA ATGTTGGATG CCAACAAGAT TCAGAAGAAC TGTTGAAACT AGCAACATCT 
ATAAGTGAAA CCTTGGAAGA GAAGCCTGAT GTAAATGCTG ACATTGTGCA TTGGCTCTCT 
TGGACTGCCC AAGGCTTTTT ATCTCCACTT GCTGCAGCAG TAGGAGGTGT TGCCAGCCAA 
GAAGTATTGA AAGCTGTAAC AGGAAAATTT TCTCCTTTGT GCCAGTGGTT ATATCTTGAA 
GCAGCAGATA TTGTTGAATC ACTAGGCAAA CCTGAATGTG AAGAATTTCT CCCACGAGGA 
GATAGATATG ATGCCTTAAG AGCTTGCATT GGAGACACTT TGTGTCAGAA ACTGCAAAAT 
TTAAACATCT TCTTAGTAGG GTGTGGAGCC ATAGGCTGTG AAATGTTGAA AAATTTTGCT 
TTACTTGGTG TTGGCACAAG CAAAGAGAAA GGAATGATTA CAGTTACAGA TCCTGACTTG 
ATAGAGAAAT CCAACTTAAA TAGACAGTTC CTATTTCGTC CTCATCACAT ACAGAAACCT 
AAAAGCTACA CTGCTGCTGA TGCTACTCTG AAAATAAATT CTCAAATAAA GATAGATGCA 
CACCTGAACA AAGTATGTCC AACCACTGAG ACCATTTACA ATGATGAGTT CTATACTAAA 
CAAGATGTAA TTATTACAGC ATTAGATAAT GTGGAAGCCA GGAGATACGT AGACAGTCGT 
TGCTTAGCAA ATCTAAGGCC TCTTTTAGAT TCTGGAACAA TGGGCACTAA GGGACACACT 
GAAGTTATTG TACCGCATTT GACTGAGTCT TACAATAGTC ATCGGGATCC CCCAGAAGAG 
GAAATACCAT TTTGTACTCT AAAATCCTTT CCAGCTGCTA TTGAACATAC CATACAGTGG 
GCAAGAGATA AGTTTGAAAG TTCCTTTTCC CACAAACCTT CATTGTTTAA CAAATTTTGG 
CAAACCTATT CATCTGCAGA AGAAGTCTTA CAGAAGATAC AGAGTGGACA CAGTTTAGAA 
GGCTGTTTTC AAGTTATAAA GTTACTTAGC AGAAGACCTA GAAATTGGTC CCAGTGTGTA 
GAATTAGCAA GATTAAAGTT TGAAAAATAT TTTAACCATA AGGCTCTTCA GCTTCTTCAC 
TGTTTCCCTC TGGACATACG ATTAAAAGAT GGCAGTTTAT TTTGGCAGTC ACCAAAGAGG 
CCACCCTCTC CAATAAAATT TGATTTAAAT GAGCCTTTGC ACCTCAGTTT CCTTCAGAAT 
GCTGCAAAAC TATATGCTAC AGTATATTGT ATTCCATTTG CAGAAGAGGA CTTATCAGCA 
GATGCCCTCT TGAATATTCT TTCAGAAGTA AAGATTCAGG AATTCAAGCC TTCCAATAAG 
GTTGTTCAAA CAGATGAAAC TGCAAGGAAA CCAGACCATG TTCCTATTAG CAGTGAAGAT 
GAGAGGAATG CAATTTTCCA ACTAGAAAAG GCTATTTTAT CTAATGAAGC CACCAAAAGT 
GACCTTCAGA TGGCAGTGCT TTCATTTGAA AAAGATGATG ATCATAATGG ACACATAGAT 
TTCATCACAG CTGCATCAAA TCTTCGTGCC AAAATGTACA GCATTGAACC AGCTGACCGT 
TTCAAAACAA AGCGCATAGC TGGTAAAATT ATACCTGCTA TAGCAACAAC CACTGCTACA 
GTTTCTGGCT TGGTTGCCTT GGAGATGATC AAAGTAACTG GTGGCTATCC ATTTGAAGCT 
TACAAAAATT GTTTTCTTAA CTTAGCCATT CCAATTGTAG TATTTACAGA GACAACTGAA 
GTAAGGAAAA CTAAAATCAG AAATGGAATA TCATTTACAA TTTGGGATCG ATGGACCGTA 
CATGGAAAAG AAGATTTCAC CCTCTTGGAT TTCATAAATG CAGTCAAAGA GAAGTATGGA 
ATTGAGCCAA CAATGGTGGT ACAGGGAGTC AAAATGCTTT ATGTTCCTGT AATGCCTGGT 
CATGCAAAAA GATTGAAGTT AACACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Orthogonal ubiquitin transfer identifies ubiquitination substrates under differential control by the two ubiquitin activating enzymes.
Nature communications814286(2017 Jan)
Liu X,Zhao B,Sun L,Bhuripanyo K,Wang Y,Bi Y,Davuluri RV,Duong DM,Nanavati D,Yin J,Kiyokawa H

LMO2 blocks the UBA6-USE1 interaction and downstream FAT10ylation by targeting the ubiquitin fold domain of UBA6.
Biochemical and biophysical research communications478(3)1442-8(2016 09)
Wu C,Liu Y,Gu X,Zhu T,Yang S,Sun W

Interstitial microdeletions including the chromosome band 4q13.2 and the UBA6 gene as possible causes of intellectual disability and behavior disorder.
American journal of medical genetics. Part A167A(12)3113-20(2015 Dec)
Quintela I,Barros F,Fernandez-Prieto M,Martinez-Regueiro R,Castro-Gago M,Carracedo A,Gomez-Lado C,Eiris J

Disruption of FAT10-MAD2 binding inhibits tumor progression.
Proceedings of the National Academy of Sciences of the United States of America111(49)E5282-91(2014 Dec)
Theng SS,Wang W,Mah WC,Chan C,Zhuo J,Gao Y,Qin H,Lim L,Chong SS,Song J,Lee CG

The Escherichia coli adherence factor plasmid of enteropathogenic Escherichia coli causes a global decrease in ubiquitylated host cell proteins by decreasing ubiquitin E1 enzyme expression through host aspartyl proteases.
The international journal of biochemistry & cell biology44(12)2223-32(2012 Dec)
Lin AE,Guttman JA

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UBA6 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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