PPP2R1A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPP2R1A
Entrez Gene ID	5518
Full Name	protein phosphatase 2 scaffold subunit Aalpha
Synonyms	MRD36,PP2A-Aalpha,PP2AA,PP2AAALPHA,PR65A
General protein information	Preferred Names protein phosphatase 2 scaffold subunit Aalpha Names serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform PP2A subunit A isoform PR65-alpha PP2A subunit A isoform R1-alpha medium tumor antigen-associated 61 KDA protein protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform protein phosphatase 2, regulatory subunit A, alpha protein phosphatase 2A structural subunit A, alpha isoform serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, alpha isoform testicular secretory protein Li 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.41
Summary	This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010].
Disorder MIM:	605983
Disorder Html:	Mental retardation, autosomal dominant 36, 616362 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017026929.1	XP_016882418.1	serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform isoform X1
NM_001363656.2	NP_001350585.1	serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform isoform 2
NM_014225.5	NP_055040.2	serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
NM_001363656.1	NP_001350585.1	serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform isoform 2
NM_014225.6	NP_055040.2	serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform isoform 1

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ACR279C	NP_983681.1
Neurospora crassa	NCU00488	XP_001728513.1
Bos taurus (cattle)	PPP2R1A	NP_001032554.1
Mus musculus (house mouse)	Ppp2r1a	NP_058587.1
Drosophila melanogaster (fruit fly)	Pp2A-29B	NP_001027225.1
Arabidopsis thaliana (thale cress)	RCN1	NP_173920.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009105	XP_319856.4
Kluyveromyces lactis	KLLA0F07689g	XP_455428.1
Schizosaccharomyces pombe (fission yeast)	paa1	NP_594948.1
Homo sapiens (human)	PPP2R1A	NP_055040.2
Pan troglodytes (chimpanzee)	PPP2R1A	XP_001174546.1
	PPP2R1A	XP_001116604.1
Canis lupus familiaris (dog)	LOC484335	XP_850993.1
Rattus norvegicus (Norway rat)	Ppp2r1a	NP_476481.1
Caenorhabditis elegans (roundworm)	paa-1	NP_498162.2
Magnaporthe oryzae (rice blast fungus)	MGG_05671	XP_003710570.1
Xenopus tropicalis (tropical clawed frog)	ppp2r1a	NP_989405.1
Saccharomyces cerevisiae (baker's yeast)	TPD3	NP_009386.1

Gene Ontology
Bibliography

Related articles in PubMed

Protein Phosphatase 2A (PP2A) Regulates EG5 to Control Mitotic Progression.
Liu Y, Zhang Z, Liang H, Zhao X, Liang L, Wang G, Yang J, Jin Y, McNutt MA, Yin Y
Scientific reports7(1)1630(2017 May)

Systematic Analysis of Human Protein Phosphatase Interactions and Dynamics.
Yadav L, Tamene F, G??s H, van Drogen A, Katainen R, Aebersold R, Gstaiger M, Varjosalo M
Cell systems4(4)430-444.e5(2017 Apr)

Oncoprotein CIP2A is stabilized via interaction with tumor suppressor PP2A/B56.
Wang J, Okkeri J, Pavic K, Wang Z, Kauko O, Halonen T, Sarek G, Ojala PM, Rao Z, Xu W, Westermarck J
EMBO reports18(3)437-450(2017 03)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results demonstrated that the promotive effect of eEF-2K on glycolysis resulted from the kinase-mediated restriction of synthesis of the protein phosphatase 2A-A (PP2A-A).
Title: eEF-2 kinase is a critical regulator of Warburg effect through controlling PP2A-A synthesis.

PPP2R1A mutations occur in a subset of gastrointestinal stromal tumors and are associated with a high malignant potential that leads to decreased disease-free survival and overall survival
Title: Clinicopathological effects of protein phosphatase 2, regulatory subunit A, alpha mutations in gastrointestinal stromal tumors.

PPP2R1A mutations affect PP2A function and oncogenic signaling, illuminating the genetic basis for serous EC development.
Title: Recurrent PPP2R1A Mutations in Uterine Cancer Act through a Dominant-Negative Mechanism to Promote Malignant Cell Growth.

PPP2R1A mutation is associated with endometrial carcinoma progression and abdominopelvic metastasis.
Title: The genomic landscape and evolution of endometrial carcinoma progression and abdominopelvic metastasis.

Our results suggest that IH-induced ROS generation increases PP2A activation and subsequently downregulates ERK1/2 activation, which results in inhibition of PC12 cell proliferation through G0/G1 phase arrest and NGF-induced neuronal differentiation.
Title: Intermittent hypoxia-induced protein phosphatase 2A activation reduces PC12 cell proliferation and differentiation.

The following PPP2R1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPP2R1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu32300	NM_014225.6 Latest version!	Homo sapiens protein phosphatase 2 scaffold subunit Aalpha (PPP2R1A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu88542	NM_001363656.2 Latest version!	Homo sapiens protein phosphatase 2 scaffold subunit Aalpha (PPP2R1A), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu88542	XM_017026929.1 Latest version!	Homo sapiens protein phosphatase 2 scaffold subunit Aalpha (PPP2R1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu88542	NM_001363656.1	Homo sapiens protein phosphatase 2 scaffold subunit Aalpha (PPP2R1A), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu32300	NM_014225.5	Homo sapiens protein phosphatase 2 scaffold subunit Aalpha (PPP2R1A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPP2R1A ( NM_014225.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_055040.2 Homo sapiens protein phosphatase 2 scaffold subunit Aalpha (PPP2R1A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu32300
Clone ID Related Accession (Same CDS sequence)	NM_014225.5 , NM_014225.6
Accession Version	NM_014225.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC398468.1, BC026733.1 and CA426613.1. This sequence is a reference standard in the RefSeqGene project. On Apr 16, 2010 this sequence version replaced NM_014225.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.141658.1, HM005541.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CCGACGGCGA CGACTCGCTG TACCCCATCG CGGTGCTCAT AGACGAACTC 
CGCAATGAGG ACGTTCAGCT TCGCCTCAAC AGCATCAAGA AGCTGTCCAC CATCGCCTTG 
GCCCTTGGGG TTGAAAGGAC CCGAAGTGAG CTTCTGCCTT TCCTTACAGA TACCATCTAT 
GATGAAGATG AGGTCCTCCT GGCCCTGGCA GAACAGCTGG GAACCTTCAC TACCCTGGTG 
GGAGGCCCAG AGTACGTGCA CTGCCTGCTG CCACCGCTGG AGTCGCTGGC CACAGTGGAG 
GAGACAGTGG TGCGGGACAA GGCAGTGGAG TCCTTACGGG CCATCTCACA CGAGCACTCG 
CCCTCTGACC TGGAGGCGCA CTTTGTGCCG CTAGTGAAGC GGCTGGCGGG CGGCGACTGG 
TTCACCTCCC GCACCTCGGC CTGCGGCCTC TTCTCCGTCT GCTACCCCCG AGTGTCCAGT 
GCTGTGAAGG CGGAACTTCG ACAGTACTTC CGGAACCTGT GCTCAGATGA CACCCCCATG 
GTGCGGCGGG CCGCAGCCTC CAAGCTGGGG GAGTTTGCCA AGGTGCTGGA GCTGGACAAC 
GTCAAGAGTG AGATCATCCC CATGTTCTCC AACCTGGCCT CTGACGAGCA GGACTCGGTG 
CGGCTGCTGG CGGTGGAGGC GTGCGTGAAC ATCGCCCAGC TTCTGCCCCA GGAGGATCTG 
GAGGCCCTGG TGATGCCCAC TCTGCGCCAG GCCGCTGAAG ACAAGTCCTG GCGCGTCCGC 
TACATGGTGG CTGACAAGTT CACAGAGCTC CAGAAAGCAG TGGGGCCTGA GATCACCAAG 
ACAGACCTGG TCCCTGCCTT CCAGAACCTG ATGAAAGACT GTGAGGCCGA GGTGAGGGCC 
GCAGCCTCCC ACAAGGTCAA AGAGTTCTGT GAAAACCTCT CAGCTGACTG TCGGGAGAAT 
GTGATCATGT CCCAGATCTT GCCCTGCATC AAGGAGCTGG TGTCCGATGC CAACCAACAT 
GTCAAGTCTG CCCTGGCCTC AGTCATCATG GGTCTCTCTC CCATCTTGGG CAAAGACAAC 
ACCATCGAGC ACCTCTTGCC CCTCTTCCTG GCTCAGCTGA AGGATGAGTG CCCTGAGGTA 
CGGCTGAACA TCATCTCTAA CCTGGACTGT GTGAACGAGG TGATTGGCAT CCGGCAGCTG 
TCCCAGTCCC TGCTCCCTGC CATTGTGGAG CTGGCTGAGG ACGCCAAGTG GCGGGTGCGG 
CTGGCCATCA TTGAGTACAT GCCCCTCCTG GCTGGACAGC TGGGAGTGGA GTTCTTTGAT 
GAGAAACTTA ACTCCTTGTG CATGGCCTGG CTTGTGGATC ATGTATATGC CATCCGCGAG 
GCAGCCACCA GCAACCTGAA GAAGCTAGTG GAAAAGTTTG GGAAGGAGTG GGCCCATGCC 
ACAATCATCC CCAAGGTCTT GGCCATGTCC GGAGACCCCA ACTACCTGCA CCGCATGACT 
ACGCTCTTCT GCATCAATGT GCTGTCTGAG GTCTGTGGGC AGGACATCAC CACCAAGCAC 
ATGCTACCCA CGGTTCTGCG CATGGCTGGG GACCCGGTTG CCAATGTCCG CTTCAATGTG 
GCCAAGTCTC TGCAGAAGAT AGGGCCCATC CTGGACAACA GCACCTTGCA GAGTGAAGTC 
AAGCCCATCC TAGAGAAGCT GACCCAGGAC CAGGATGTGG ACGTCAAATA CTTTGCCCAG 
GAGGCTCTGA CTGTTCTGTC TCTCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055040.2
CDS	296..2065
Translation	MAAADGDDSLYPIAVLIDELRNEDVQLRLNSIKKLSTIALALGVERTRSELLPFLTDTIYDEDEVLLALAEQLGTFTTLVGGPEYVHCLLPPLESLATVEETVVRDKAVESLRAISHEHSPSDLEAHFVPLVKRLAGGDWFTSRTSACGLFSVCYPRVSSAVKAELRQYFRNLCSDDTPMVRRAAASKLGEFAKVLELDNVKSEIIPMFSNLASDEQDSVRLLAVEACVNIAQLLPQEDLEALVMPTLRQAAEDKSWRVRYMVADKFTELQKAVGPEITKTDLVPAFQNLMKDCEAEVRAAASHKVKEFCENLSADCRENVIMSQILPCIKELVSDANQHVKSALASVIMGLSPILGKDNTIEHLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKFGKEWAHATIIPKVLAMSGDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTVLRMAGDPVANVRFNVAKSLQKIGPILDNSTLQSEVKPILEKLTQDQDVDVKYFAQEALTVLSLA

Target ORF information:

RefSeq Version	NM_014225.5
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 2 scaffold subunit Aalpha (PPP2R1A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014225.5

ORF Insert Sequence:

ATGGCGGCGG CCGACGGCGA CGACTCGCTG TACCCCATCG CGGTGCTCAT AGACGAACTC 
CGCAATGAGG ACGTTCAGCT TCGCCTCAAC AGCATCAAGA AGCTGTCCAC CATCGCCTTG 
GCCCTTGGGG TTGAAAGGAC CCGAAGTGAG CTTCTGCCTT TCCTTACAGA TACCATCTAT 
GATGAAGATG AGGTCCTCCT GGCCCTGGCA GAACAGCTGG GAACCTTCAC TACCCTGGTG 
GGAGGCCCAG AGTACGTGCA CTGCCTGCTG CCACCGCTGG AGTCGCTGGC CACAGTGGAG 
GAGACAGTGG TGCGGGACAA GGCAGTGGAG TCCTTACGGG CCATCTCACA CGAGCACTCG 
CCCTCTGACC TGGAGGCGCA CTTTGTGCCG CTAGTGAAGC GGCTGGCGGG CGGCGACTGG 
TTCACCTCCC GCACCTCGGC CTGCGGCCTC TTCTCCGTCT GCTACCCCCG AGTGTCCAGT 
GCTGTGAAGG CGGAACTTCG ACAGTACTTC CGGAACCTGT GCTCAGATGA CACCCCCATG 
GTGCGGCGGG CCGCAGCCTC CAAGCTGGGG GAGTTTGCCA AGGTGCTGGA GCTGGACAAC 
GTCAAGAGTG AGATCATCCC CATGTTCTCC AACCTGGCCT CTGACGAGCA GGACTCGGTG 
CGGCTGCTGG CGGTGGAGGC GTGCGTGAAC ATCGCCCAGC TTCTGCCCCA GGAGGATCTG 
GAGGCCCTGG TGATGCCCAC TCTGCGCCAG GCCGCTGAAG ACAAGTCCTG GCGCGTCCGC 
TACATGGTGG CTGACAAGTT CACAGAGCTC CAGAAAGCAG TGGGGCCTGA GATCACCAAG 
ACAGACCTGG TCCCTGCCTT CCAGAACCTG ATGAAAGACT GTGAGGCCGA GGTGAGGGCC 
GCAGCCTCCC ACAAGGTCAA AGAGTTCTGT GAAAACCTCT CAGCTGACTG TCGGGAGAAT 
GTGATCATGT CCCAGATCTT GCCCTGCATC AAGGAGCTGG TGTCCGATGC CAACCAACAT 
GTCAAGTCTG CCCTGGCCTC AGTCATCATG GGTCTCTCTC CCATCTTGGG CAAAGACAAC 
ACCATCGAGC ACCTCTTGCC CCTCTTCCTG GCTCAGCTGA AGGATGAGTG CCCTGAGGTA 
CGGCTGAACA TCATCTCTAA CCTGGACTGT GTGAACGAGG TGATTGGCAT CCGGCAGCTG 
TCCCAGTCCC TGCTCCCTGC CATTGTGGAG CTGGCTGAGG ACGCCAAGTG GCGGGTGCGG 
CTGGCCATCA TTGAGTACAT GCCCCTCCTG GCTGGACAGC TGGGAGTGGA GTTCTTTGAT 
GAGAAACTTA ACTCCTTGTG CATGGCCTGG CTTGTGGATC ATGTATATGC CATCCGCGAG 
GCAGCCACCA GCAACCTGAA GAAGCTAGTG GAAAAGTTTG GGAAGGAGTG GGCCCATGCC 
ACAATCATCC CCAAGGTCTT GGCCATGTCC GGAGACCCCA ACTACCTGCA CCGCATGACT 
ACGCTCTTCT GCATCAATGT GCTGTCTGAG GTCTGTGGGC AGGACATCAC CACCAAGCAC 
ATGCTACCCA CGGTTCTGCG CATGGCTGGG GACCCGGTTG CCAATGTCCG CTTCAATGTG 
GCCAAGTCTC TGCAGAAGAT AGGGCCCATC CTGGACAACA GCACCTTGCA GAGTGAAGTC 
AAGCCCATCC TAGAGAAGCT GACCCAGGAC CAGGATGTGG ACGTCAAATA CTTTGCCCAG 
GAGGCTCTGA CTGTTCTGTC TCTCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP2R1A ( NM_014225.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_055040.2 Homo sapiens protein phosphatase 2 scaffold subunit Aalpha (PPP2R1A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu32300
Clone ID Related Accession (Same CDS sequence)	NM_014225.5 , NM_014225.6
Accession Version	NM_014225.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC026733.1, CA426613.1 and AC010320.10. On Nov 22, 2018 this sequence version replaced NM_014225.5. Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.57023.1, SRR1803611.188780.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000322088.11/ ENSP00000324804.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCGG CCGACGGCGA CGACTCGCTG TACCCCATCG CGGTGCTCAT AGACGAACTC 
CGCAATGAGG ACGTTCAGCT TCGCCTCAAC AGCATCAAGA AGCTGTCCAC CATCGCCTTG 
GCCCTTGGGG TTGAAAGGAC CCGAAGTGAG CTTCTGCCTT TCCTTACAGA TACCATCTAT 
GATGAAGATG AGGTCCTCCT GGCCCTGGCA GAACAGCTGG GAACCTTCAC TACCCTGGTG 
GGAGGCCCAG AGTACGTGCA CTGCCTGCTG CCACCGCTGG AGTCGCTGGC CACAGTGGAG 
GAGACAGTGG TGCGGGACAA GGCAGTGGAG TCCTTACGGG CCATCTCACA CGAGCACTCG 
CCCTCTGACC TGGAGGCGCA CTTTGTGCCG CTAGTGAAGC GGCTGGCGGG CGGCGACTGG 
TTCACCTCCC GCACCTCGGC CTGCGGCCTC TTCTCCGTCT GCTACCCCCG AGTGTCCAGT 
GCTGTGAAGG CGGAACTTCG ACAGTACTTC CGGAACCTGT GCTCAGATGA CACCCCCATG 
GTGCGGCGGG CCGCAGCCTC CAAGCTGGGG GAGTTTGCCA AGGTGCTGGA GCTGGACAAC 
GTCAAGAGTG AGATCATCCC CATGTTCTCC AACCTGGCCT CTGACGAGCA GGACTCGGTG 
CGGCTGCTGG CGGTGGAGGC GTGCGTGAAC ATCGCCCAGC TTCTGCCCCA GGAGGATCTG 
GAGGCCCTGG TGATGCCCAC TCTGCGCCAG GCCGCTGAAG ACAAGTCCTG GCGCGTCCGC 
TACATGGTGG CTGACAAGTT CACAGAGCTC CAGAAAGCAG TGGGGCCTGA GATCACCAAG 
ACAGACCTGG TCCCTGCCTT CCAGAACCTG ATGAAAGACT GTGAGGCCGA GGTGAGGGCC 
GCAGCCTCCC ACAAGGTCAA AGAGTTCTGT GAAAACCTCT CAGCTGACTG TCGGGAGAAT 
GTGATCATGT CCCAGATCTT GCCCTGCATC AAGGAGCTGG TGTCCGATGC CAACCAACAT 
GTCAAGTCTG CCCTGGCCTC AGTCATCATG GGTCTCTCTC CCATCTTGGG CAAAGACAAC 
ACCATCGAGC ACCTCTTGCC CCTCTTCCTG GCTCAGCTGA AGGATGAGTG CCCTGAGGTA 
CGGCTGAACA TCATCTCTAA CCTGGACTGT GTGAACGAGG TGATTGGCAT CCGGCAGCTG 
TCCCAGTCCC TGCTCCCTGC CATTGTGGAG CTGGCTGAGG ACGCCAAGTG GCGGGTGCGG 
CTGGCCATCA TTGAGTACAT GCCCCTCCTG GCTGGACAGC TGGGAGTGGA GTTCTTTGAT 
GAGAAACTTA ACTCCTTGTG CATGGCCTGG CTTGTGGATC ATGTATATGC CATCCGCGAG 
GCAGCCACCA GCAACCTGAA GAAGCTAGTG GAAAAGTTTG GGAAGGAGTG GGCCCATGCC 
ACAATCATCC CCAAGGTCTT GGCCATGTCC GGAGACCCCA ACTACCTGCA CCGCATGACT 
ACGCTCTTCT GCATCAATGT GCTGTCTGAG GTCTGTGGGC AGGACATCAC CACCAAGCAC 
ATGCTACCCA CGGTTCTGCG CATGGCTGGG GACCCGGTTG CCAATGTCCG CTTCAATGTG 
GCCAAGTCTC TGCAGAAGAT AGGGCCCATC CTGGACAACA GCACCTTGCA GAGTGAAGTC 
AAGCCCATCC TAGAGAAGCT GACCCAGGAC CAGGATGTGG ACGTCAAATA CTTTGCCCAG 
GAGGCTCTGA CTGTTCTGTC TCTCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055040.2
CDS	46..1815
Translation	MAAADGDDSLYPIAVLIDELRNEDVQLRLNSIKKLSTIALALGVERTRSELLPFLTDTIYDEDEVLLALAEQLGTFTTLVGGPEYVHCLLPPLESLATVEETVVRDKAVESLRAISHEHSPSDLEAHFVPLVKRLAGGDWFTSRTSACGLFSVCYPRVSSAVKAELRQYFRNLCSDDTPMVRRAAASKLGEFAKVLELDNVKSEIIPMFSNLASDEQDSVRLLAVEACVNIAQLLPQEDLEALVMPTLRQAAEDKSWRVRYMVADKFTELQKAVGPEITKTDLVPAFQNLMKDCEAEVRAAASHKVKEFCENLSADCRENVIMSQILPCIKELVSDANQHVKSALASVIMGLSPILGKDNTIEHLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKFGKEWAHATIIPKVLAMSGDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTVLRMAGDPVANVRFNVAKSLQKIGPILDNSTLQSEVKPILEKLTQDQDVDVKYFAQEALTVLSLA

Target ORF information:

RefSeq Version	NM_014225.6
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 2 scaffold subunit Aalpha (PPP2R1A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014225.6

ORF Insert Sequence:

ATGGCGGCGG CCGACGGCGA CGACTCGCTG TACCCCATCG CGGTGCTCAT AGACGAACTC 
CGCAATGAGG ACGTTCAGCT TCGCCTCAAC AGCATCAAGA AGCTGTCCAC CATCGCCTTG 
GCCCTTGGGG TTGAAAGGAC CCGAAGTGAG CTTCTGCCTT TCCTTACAGA TACCATCTAT 
GATGAAGATG AGGTCCTCCT GGCCCTGGCA GAACAGCTGG GAACCTTCAC TACCCTGGTG 
GGAGGCCCAG AGTACGTGCA CTGCCTGCTG CCACCGCTGG AGTCGCTGGC CACAGTGGAG 
GAGACAGTGG TGCGGGACAA GGCAGTGGAG TCCTTACGGG CCATCTCACA CGAGCACTCG 
CCCTCTGACC TGGAGGCGCA CTTTGTGCCG CTAGTGAAGC GGCTGGCGGG CGGCGACTGG 
TTCACCTCCC GCACCTCGGC CTGCGGCCTC TTCTCCGTCT GCTACCCCCG AGTGTCCAGT 
GCTGTGAAGG CGGAACTTCG ACAGTACTTC CGGAACCTGT GCTCAGATGA CACCCCCATG 
GTGCGGCGGG CCGCAGCCTC CAAGCTGGGG GAGTTTGCCA AGGTGCTGGA GCTGGACAAC 
GTCAAGAGTG AGATCATCCC CATGTTCTCC AACCTGGCCT CTGACGAGCA GGACTCGGTG 
CGGCTGCTGG CGGTGGAGGC GTGCGTGAAC ATCGCCCAGC TTCTGCCCCA GGAGGATCTG 
GAGGCCCTGG TGATGCCCAC TCTGCGCCAG GCCGCTGAAG ACAAGTCCTG GCGCGTCCGC 
TACATGGTGG CTGACAAGTT CACAGAGCTC CAGAAAGCAG TGGGGCCTGA GATCACCAAG 
ACAGACCTGG TCCCTGCCTT CCAGAACCTG ATGAAAGACT GTGAGGCCGA GGTGAGGGCC 
GCAGCCTCCC ACAAGGTCAA AGAGTTCTGT GAAAACCTCT CAGCTGACTG TCGGGAGAAT 
GTGATCATGT CCCAGATCTT GCCCTGCATC AAGGAGCTGG TGTCCGATGC CAACCAACAT 
GTCAAGTCTG CCCTGGCCTC AGTCATCATG GGTCTCTCTC CCATCTTGGG CAAAGACAAC 
ACCATCGAGC ACCTCTTGCC CCTCTTCCTG GCTCAGCTGA AGGATGAGTG CCCTGAGGTA 
CGGCTGAACA TCATCTCTAA CCTGGACTGT GTGAACGAGG TGATTGGCAT CCGGCAGCTG 
TCCCAGTCCC TGCTCCCTGC CATTGTGGAG CTGGCTGAGG ACGCCAAGTG GCGGGTGCGG 
CTGGCCATCA TTGAGTACAT GCCCCTCCTG GCTGGACAGC TGGGAGTGGA GTTCTTTGAT 
GAGAAACTTA ACTCCTTGTG CATGGCCTGG CTTGTGGATC ATGTATATGC CATCCGCGAG 
GCAGCCACCA GCAACCTGAA GAAGCTAGTG GAAAAGTTTG GGAAGGAGTG GGCCCATGCC 
ACAATCATCC CCAAGGTCTT GGCCATGTCC GGAGACCCCA ACTACCTGCA CCGCATGACT 
ACGCTCTTCT GCATCAATGT GCTGTCTGAG GTCTGTGGGC AGGACATCAC CACCAAGCAC 
ATGCTACCCA CGGTTCTGCG CATGGCTGGG GACCCGGTTG CCAATGTCCG CTTCAATGTG 
GCCAAGTCTC TGCAGAAGAT AGGGCCCATC CTGGACAACA GCACCTTGCA GAGTGAAGTC 
AAGCCCATCC TAGAGAAGCT GACCCAGGAC CAGGATGTGG ACGTCAAATA CTTTGCCCAG 
GAGGCTCTGA CTGTTCTGTC TCTCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP2R1A ( NM_001363656.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350585.1 Homo sapiens protein phosphatase 2 scaffold subunit Aalpha (PPP2R1A), transcript variant 3, mRNA.

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CloneID	OHu88542
Clone ID Related Accession (Same CDS sequence)	XM_017026929.1 , NM_001363656.2 , NM_001363656.1
Accession Version	NM_001363656.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK090488.1, BC026733.1, CA426613.1 and AC010320.10. On May 31, 2019 this sequence version replaced NM_001363656.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK090488.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCGGC GGGCCGCAGC CTCCAAGCTG GGGGAGTTTG CCAAGGTGCT GGAGCTGGAC 
AACGTCAAGA GTGAGATCAT CCCCATGTTC TCCAACCTGG CCTCTGACGA GCAGGACTCG 
GTGCGGCTGC TGGCGGTGGA GGCGTGCGTG AACATCGCCC AGCTTCTGCC CCAGGAGGAT 
CTGGAGGCCC TGGTGATGCC CACTCTGCGC CAGGCCGCTG AAGACAAGTC CTGGCGCGTC 
CGCTACATGG TGGCTGACAA GTTCACAGAG CTCCAGAAAG CAGTGGGGCC TGAGATCACC 
AAGACAGACC TGGTCCCTGC CTTCCAGAAC CTGATGAAAG ACTGTGAGGC CGAGGTGAGG 
GCCGCAGCCT CCCACAAGGT CAAAGAGTTC TGTGAAAACC TCTCAGCTGA CTGTCGGGAG 
AATGTGATCA TGTCCCAGAT CTTGCCCTGC ATCAAGGAGC TGGTGTCCGA TGCCAACCAA 
CATGTCAAGT CTGCCCTGGC CTCAGTCATC ATGGGTCTCT CTCCCATCTT GGGCAAAGAC 
AACACCATCG AGCACCTCTT GCCCCTCTTC CTGGCTCAGC TGAAGGATGA GTGCCCTGAG 
GTACGGCTGA ACATCATCTC TAACCTGGAC TGTGTGAACG AGGTGATTGG CATCCGGCAG 
CTGTCCCAGT CCCTGCTCCC TGCCATTGTG GAGCTGGCTG AGGACGCCAA GTGGCGGGTG 
CGGCTGGCCA TCATTGAGTA CATGCCCCTC CTGGCTGGAC AGCTGGGAGT GGAGTTCTTT 
GATGAGAAAC TTAACTCCTT GTGCATGGCC TGGCTTGTGG ATCATGTATA TGCCATCCGC 
GAGGCAGCCA CCAGCAACCT GAAGAAGCTA GTGGAAAAGT TTGGGAAGGA GTGGGCCCAT 
GCCACAATCA TCCCCAAGGT CTTGGCCATG TCCGGAGACC CCAACTACCT GCACCGCATG 
ACTACGCTCT TCTGCATCAA TGTGCTGTCT GAGGTCTGTG GGCAGGACAT CACCACCAAG 
CACATGCTAC CCACGGTTCT GCGCATGGCT GGGGACCCGG TTGCCAATGT CCGCTTCAAT 
GTGGCCAAGT CTCTGCAGAA GATAGGGCCC ATCCTGGACA ACAGCACCTT GCAGAGTGAA 
GTCAAGCCCA TCCTAGAGAA GCTGACCCAG GACCAGGATG TGGACGTCAA ATACTTTGCC 
CAGGAGGCTC TGACTGTTCT GTCTCTCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350585.1
CDS	959..2191
Translation	MVRRAAASKLGEFAKVLELDNVKSEIIPMFSNLASDEQDSVRLLAVEACVNIAQLLPQEDLEALVMPTLRQAAEDKSWRVRYMVADKFTELQKAVGPEITKTDLVPAFQNLMKDCEAEVRAAASHKVKEFCENLSADCRENVIMSQILPCIKELVSDANQHVKSALASVIMGLSPILGKDNTIEHLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKFGKEWAHATIIPKVLAMSGDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTVLRMAGDPVANVRFNVAKSLQKIGPILDNSTLQSEVKPILEKLTQDQDVDVKYFAQEALTVLSLA

Target ORF information:

RefSeq Version	NM_001363656.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 2 scaffold subunit Aalpha (PPP2R1A), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363656.2

ORF Insert Sequence:

ATGGTGCGGC GGGCCGCAGC CTCCAAGCTG GGGGAGTTTG CCAAGGTGCT GGAGCTGGAC 
AACGTCAAGA GTGAGATCAT CCCCATGTTC TCCAACCTGG CCTCTGACGA GCAGGACTCG 
GTGCGGCTGC TGGCGGTGGA GGCGTGCGTG AACATCGCCC AGCTTCTGCC CCAGGAGGAT 
CTGGAGGCCC TGGTGATGCC CACTCTGCGC CAGGCCGCTG AAGACAAGTC CTGGCGCGTC 
CGCTACATGG TGGCTGACAA GTTCACAGAG CTCCAGAAAG CAGTGGGGCC TGAGATCACC 
AAGACAGACC TGGTCCCTGC CTTCCAGAAC CTGATGAAAG ACTGTGAGGC CGAGGTGAGG 
GCCGCAGCCT CCCACAAGGT CAAAGAGTTC TGTGAAAACC TCTCAGCTGA CTGTCGGGAG 
AATGTGATCA TGTCCCAGAT CTTGCCCTGC ATCAAGGAGC TGGTGTCCGA TGCCAACCAA 
CATGTCAAGT CTGCCCTGGC CTCAGTCATC ATGGGTCTCT CTCCCATCTT GGGCAAAGAC 
AACACCATCG AGCACCTCTT GCCCCTCTTC CTGGCTCAGC TGAAGGATGA GTGCCCTGAG 
GTACGGCTGA ACATCATCTC TAACCTGGAC TGTGTGAACG AGGTGATTGG CATCCGGCAG 
CTGTCCCAGT CCCTGCTCCC TGCCATTGTG GAGCTGGCTG AGGACGCCAA GTGGCGGGTG 
CGGCTGGCCA TCATTGAGTA CATGCCCCTC CTGGCTGGAC AGCTGGGAGT GGAGTTCTTT 
GATGAGAAAC TTAACTCCTT GTGCATGGCC TGGCTTGTGG ATCATGTATA TGCCATCCGC 
GAGGCAGCCA CCAGCAACCT GAAGAAGCTA GTGGAAAAGT TTGGGAAGGA GTGGGCCCAT 
GCCACAATCA TCCCCAAGGT CTTGGCCATG TCCGGAGACC CCAACTACCT GCACCGCATG 
ACTACGCTCT TCTGCATCAA TGTGCTGTCT GAGGTCTGTG GGCAGGACAT CACCACCAAG 
CACATGCTAC CCACGGTTCT GCGCATGGCT GGGGACCCGG TTGCCAATGT CCGCTTCAAT 
GTGGCCAAGT CTCTGCAGAA GATAGGGCCC ATCCTGGACA ACAGCACCTT GCAGAGTGAA 
GTCAAGCCCA TCCTAGAGAA GCTGACCCAG GACCAGGATG TGGACGTCAA ATACTTTGCC 
CAGGAGGCTC TGACTGTTCT GTCTCTCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP2R1A ( NM_001363656.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350585.1 Homo sapiens protein phosphatase 2 scaffold subunit Aalpha (PPP2R1A), transcript variant 3, mRNA.

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CloneID	OHu88542
Clone ID Related Accession (Same CDS sequence)	XM_017026929.1 , NM_001363656.2 , NM_001363656.1
Accession Version	NM_001363656.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-27
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK090488.1, BC026733.1 and CA426613.1. On May 24, 2018 this sequence version replaced XM_017026929.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK090488.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCGGC GGGCCGCAGC CTCCAAGCTG GGGGAGTTTG CCAAGGTGCT GGAGCTGGAC 
AACGTCAAGA GTGAGATCAT CCCCATGTTC TCCAACCTGG CCTCTGACGA GCAGGACTCG 
GTGCGGCTGC TGGCGGTGGA GGCGTGCGTG AACATCGCCC AGCTTCTGCC CCAGGAGGAT 
CTGGAGGCCC TGGTGATGCC CACTCTGCGC CAGGCCGCTG AAGACAAGTC CTGGCGCGTC 
CGCTACATGG TGGCTGACAA GTTCACAGAG CTCCAGAAAG CAGTGGGGCC TGAGATCACC 
AAGACAGACC TGGTCCCTGC CTTCCAGAAC CTGATGAAAG ACTGTGAGGC CGAGGTGAGG 
GCCGCAGCCT CCCACAAGGT CAAAGAGTTC TGTGAAAACC TCTCAGCTGA CTGTCGGGAG 
AATGTGATCA TGTCCCAGAT CTTGCCCTGC ATCAAGGAGC TGGTGTCCGA TGCCAACCAA 
CATGTCAAGT CTGCCCTGGC CTCAGTCATC ATGGGTCTCT CTCCCATCTT GGGCAAAGAC 
AACACCATCG AGCACCTCTT GCCCCTCTTC CTGGCTCAGC TGAAGGATGA GTGCCCTGAG 
GTACGGCTGA ACATCATCTC TAACCTGGAC TGTGTGAACG AGGTGATTGG CATCCGGCAG 
CTGTCCCAGT CCCTGCTCCC TGCCATTGTG GAGCTGGCTG AGGACGCCAA GTGGCGGGTG 
CGGCTGGCCA TCATTGAGTA CATGCCCCTC CTGGCTGGAC AGCTGGGAGT GGAGTTCTTT 
GATGAGAAAC TTAACTCCTT GTGCATGGCC TGGCTTGTGG ATCATGTATA TGCCATCCGC 
GAGGCAGCCA CCAGCAACCT GAAGAAGCTA GTGGAAAAGT TTGGGAAGGA GTGGGCCCAT 
GCCACAATCA TCCCCAAGGT CTTGGCCATG TCCGGAGACC CCAACTACCT GCACCGCATG 
ACTACGCTCT TCTGCATCAA TGTGCTGTCT GAGGTCTGTG GGCAGGACAT CACCACCAAG 
CACATGCTAC CCACGGTTCT GCGCATGGCT GGGGACCCGG TTGCCAATGT CCGCTTCAAT 
GTGGCCAAGT CTCTGCAGAA GATAGGGCCC ATCCTGGACA ACAGCACCTT GCAGAGTGAA 
GTCAAGCCCA TCCTAGAGAA GCTGACCCAG GACCAGGATG TGGACGTCAA ATACTTTGCC 
CAGGAGGCTC TGACTGTTCT GTCTCTCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350585.1
CDS	959..2191
Translation	MVRRAAASKLGEFAKVLELDNVKSEIIPMFSNLASDEQDSVRLLAVEACVNIAQLLPQEDLEALVMPTLRQAAEDKSWRVRYMVADKFTELQKAVGPEITKTDLVPAFQNLMKDCEAEVRAAASHKVKEFCENLSADCRENVIMSQILPCIKELVSDANQHVKSALASVIMGLSPILGKDNTIEHLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKFGKEWAHATIIPKVLAMSGDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTVLRMAGDPVANVRFNVAKSLQKIGPILDNSTLQSEVKPILEKLTQDQDVDVKYFAQEALTVLSLA

Target ORF information:

RefSeq Version	NM_001363656.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 2 scaffold subunit Aalpha (PPP2R1A), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363656.1

ORF Insert Sequence:

ATGGTGCGGC GGGCCGCAGC CTCCAAGCTG GGGGAGTTTG CCAAGGTGCT GGAGCTGGAC 
AACGTCAAGA GTGAGATCAT CCCCATGTTC TCCAACCTGG CCTCTGACGA GCAGGACTCG 
GTGCGGCTGC TGGCGGTGGA GGCGTGCGTG AACATCGCCC AGCTTCTGCC CCAGGAGGAT 
CTGGAGGCCC TGGTGATGCC CACTCTGCGC CAGGCCGCTG AAGACAAGTC CTGGCGCGTC 
CGCTACATGG TGGCTGACAA GTTCACAGAG CTCCAGAAAG CAGTGGGGCC TGAGATCACC 
AAGACAGACC TGGTCCCTGC CTTCCAGAAC CTGATGAAAG ACTGTGAGGC CGAGGTGAGG 
GCCGCAGCCT CCCACAAGGT CAAAGAGTTC TGTGAAAACC TCTCAGCTGA CTGTCGGGAG 
AATGTGATCA TGTCCCAGAT CTTGCCCTGC ATCAAGGAGC TGGTGTCCGA TGCCAACCAA 
CATGTCAAGT CTGCCCTGGC CTCAGTCATC ATGGGTCTCT CTCCCATCTT GGGCAAAGAC 
AACACCATCG AGCACCTCTT GCCCCTCTTC CTGGCTCAGC TGAAGGATGA GTGCCCTGAG 
GTACGGCTGA ACATCATCTC TAACCTGGAC TGTGTGAACG AGGTGATTGG CATCCGGCAG 
CTGTCCCAGT CCCTGCTCCC TGCCATTGTG GAGCTGGCTG AGGACGCCAA GTGGCGGGTG 
CGGCTGGCCA TCATTGAGTA CATGCCCCTC CTGGCTGGAC AGCTGGGAGT GGAGTTCTTT 
GATGAGAAAC TTAACTCCTT GTGCATGGCC TGGCTTGTGG ATCATGTATA TGCCATCCGC 
GAGGCAGCCA CCAGCAACCT GAAGAAGCTA GTGGAAAAGT TTGGGAAGGA GTGGGCCCAT 
GCCACAATCA TCCCCAAGGT CTTGGCCATG TCCGGAGACC CCAACTACCT GCACCGCATG 
ACTACGCTCT TCTGCATCAA TGTGCTGTCT GAGGTCTGTG GGCAGGACAT CACCACCAAG 
CACATGCTAC CCACGGTTCT GCGCATGGCT GGGGACCCGG TTGCCAATGT CCGCTTCAAT 
GTGGCCAAGT CTCTGCAGAA GATAGGGCCC ATCCTGGACA ACAGCACCTT GCAGAGTGAA 
GTCAAGCCCA TCCTAGAGAA GCTGACCCAG GACCAGGATG TGGACGTCAA ATACTTTGCC 
CAGGAGGCTC TGACTGTTCT GTCTCTCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP2R1A ( XM_017026929.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882418.1 Homo sapiens protein phosphatase 2 scaffold subunit Aalpha (PPP2R1A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu88542
Clone ID Related Accession (Same CDS sequence)	XM_017026929.1 , NM_001363656.2 , NM_001363656.1
Accession Version	XM_017026929.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCGGC GGGCCGCAGC CTCCAAGCTG GGGGAGTTTG CCAAGGTGCT GGAGCTGGAC 
AACGTCAAGA GTGAGATCAT CCCCATGTTC TCCAACCTGG CCTCTGACGA GCAGGACTCG 
GTGCGGCTGC TGGCGGTGGA GGCGTGCGTG AACATCGCCC AGCTTCTGCC CCAGGAGGAT 
CTGGAGGCCC TGGTGATGCC CACTCTGCGC CAGGCCGCTG AAGACAAGTC CTGGCGCGTC 
CGCTACATGG TGGCTGACAA GTTCACAGAG CTCCAGAAAG CAGTGGGGCC TGAGATCACC 
AAGACAGACC TGGTCCCTGC CTTCCAGAAC CTGATGAAAG ACTGTGAGGC CGAGGTGAGG 
GCCGCAGCCT CCCACAAGGT CAAAGAGTTC TGTGAAAACC TCTCAGCTGA CTGTCGGGAG 
AATGTGATCA TGTCCCAGAT CTTGCCCTGC ATCAAGGAGC TGGTGTCCGA TGCCAACCAA 
CATGTCAAGT CTGCCCTGGC CTCAGTCATC ATGGGTCTCT CTCCCATCTT GGGCAAAGAC 
AACACCATCG AGCACCTCTT GCCCCTCTTC CTGGCTCAGC TGAAGGATGA GTGCCCTGAG 
GTACGGCTGA ACATCATCTC TAACCTGGAC TGTGTGAACG AGGTGATTGG CATCCGGCAG 
CTGTCCCAGT CCCTGCTCCC TGCCATTGTG GAGCTGGCTG AGGACGCCAA GTGGCGGGTG 
CGGCTGGCCA TCATTGAGTA CATGCCCCTC CTGGCTGGAC AGCTGGGAGT GGAGTTCTTT 
GATGAGAAAC TTAACTCCTT GTGCATGGCC TGGCTTGTGG ATCATGTATA TGCCATCCGC 
GAGGCAGCCA CCAGCAACCT GAAGAAGCTA GTGGAAAAGT TTGGGAAGGA GTGGGCCCAT 
GCCACAATCA TCCCCAAGGT CTTGGCCATG TCCGGAGACC CCAACTACCT GCACCGCATG 
ACTACGCTCT TCTGCATCAA TGTGCTGTCT GAGGTCTGTG GGCAGGACAT CACCACCAAG 
CACATGCTAC CCACGGTTCT GCGCATGGCT GGGGACCCGG TTGCCAATGT CCGCTTCAAT 
GTGGCCAAGT CTCTGCAGAA GATAGGGCCC ATCCTGGACA ACAGCACCTT GCAGAGTGAA 
GTCAAGCCCA TCCTAGAGAA GCTGACCCAG GACCAGGATG TGGACGTCAA ATACTTTGCC 
CAGGAGGCTC TGACTGTTCT GTCTCTCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882418.1
CDS	1062..2294
Translation	MVRRAAASKLGEFAKVLELDNVKSEIIPMFSNLASDEQDSVRLLAVEACVNIAQLLPQEDLEALVMPTLRQAAEDKSWRVRYMVADKFTELQKAVGPEITKTDLVPAFQNLMKDCEAEVRAAASHKVKEFCENLSADCRENVIMSQILPCIKELVSDANQHVKSALASVIMGLSPILGKDNTIEHLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKFGKEWAHATIIPKVLAMSGDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTVLRMAGDPVANVRFNVAKSLQKIGPILDNSTLQSEVKPILEKLTQDQDVDVKYFAQEALTVLSLA

Target ORF information:

RefSeq Version	XM_017026929.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 2 scaffold subunit Aalpha (PPP2R1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026929.1

ORF Insert Sequence:

ATGGTGCGGC GGGCCGCAGC CTCCAAGCTG GGGGAGTTTG CCAAGGTGCT GGAGCTGGAC 
AACGTCAAGA GTGAGATCAT CCCCATGTTC TCCAACCTGG CCTCTGACGA GCAGGACTCG 
GTGCGGCTGC TGGCGGTGGA GGCGTGCGTG AACATCGCCC AGCTTCTGCC CCAGGAGGAT 
CTGGAGGCCC TGGTGATGCC CACTCTGCGC CAGGCCGCTG AAGACAAGTC CTGGCGCGTC 
CGCTACATGG TGGCTGACAA GTTCACAGAG CTCCAGAAAG CAGTGGGGCC TGAGATCACC 
AAGACAGACC TGGTCCCTGC CTTCCAGAAC CTGATGAAAG ACTGTGAGGC CGAGGTGAGG 
GCCGCAGCCT CCCACAAGGT CAAAGAGTTC TGTGAAAACC TCTCAGCTGA CTGTCGGGAG 
AATGTGATCA TGTCCCAGAT CTTGCCCTGC ATCAAGGAGC TGGTGTCCGA TGCCAACCAA 
CATGTCAAGT CTGCCCTGGC CTCAGTCATC ATGGGTCTCT CTCCCATCTT GGGCAAAGAC 
AACACCATCG AGCACCTCTT GCCCCTCTTC CTGGCTCAGC TGAAGGATGA GTGCCCTGAG 
GTACGGCTGA ACATCATCTC TAACCTGGAC TGTGTGAACG AGGTGATTGG CATCCGGCAG 
CTGTCCCAGT CCCTGCTCCC TGCCATTGTG GAGCTGGCTG AGGACGCCAA GTGGCGGGTG 
CGGCTGGCCA TCATTGAGTA CATGCCCCTC CTGGCTGGAC AGCTGGGAGT GGAGTTCTTT 
GATGAGAAAC TTAACTCCTT GTGCATGGCC TGGCTTGTGG ATCATGTATA TGCCATCCGC 
GAGGCAGCCA CCAGCAACCT GAAGAAGCTA GTGGAAAAGT TTGGGAAGGA GTGGGCCCAT 
GCCACAATCA TCCCCAAGGT CTTGGCCATG TCCGGAGACC CCAACTACCT GCACCGCATG 
ACTACGCTCT TCTGCATCAA TGTGCTGTCT GAGGTCTGTG GGCAGGACAT CACCACCAAG 
CACATGCTAC CCACGGTTCT GCGCATGGCT GGGGACCCGG TTGCCAATGT CCGCTTCAAT 
GTGGCCAAGT CTCTGCAGAA GATAGGGCCC ATCCTGGACA ACAGCACCTT GCAGAGTGAA 
GTCAAGCCCA TCCTAGAGAA GCTGACCCAG GACCAGGATG TGGACGTCAA ATACTTTGCC 
CAGGAGGCTC TGACTGTTCT GTCTCTCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Protein Phosphatase 2A (PP2A) Regulates EG5 to Control Mitotic Progression.
Scientific reports7(1)1630(2017 May)
Liu Y,Zhang Z,Liang H,Zhao X,Liang L,Wang G,Yang J,Jin Y,McNutt MA,Yin Y

Systematic Analysis of Human Protein Phosphatase Interactions and Dynamics.
Cell systems4(4)430-444.e5(2017 Apr)
Yadav L,Tamene F,G??s H,van Drogen A,Katainen R,Aebersold R,Gstaiger M,Varjosalo M

Oncoprotein CIP2A is stabilized via interaction with tumor suppressor PP2A/B56.
EMBO reports18(3)437-450(2017 03)
Wang J,Okkeri J,Pavic K,Wang Z,Kauko O,Halonen T,Sarek G,Ojala PM,Rao Z,Xu W,Westermarck J

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PPP2R1A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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