DNAAF2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DNAAF2
Entrez Gene ID	55172
Full Name	dynein axonemal assembly factor 2
Synonyms	C14orf104,CILD10,KTU,PF13
General protein information	Preferred Names dynein axonemal assembly factor 2 Names protein kintoun dynein assembly factor 2, axonemal kintoun
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q21.3
Summary	This gene encodes a highly conserved protein involved in the preassembly of dynein arm complexes which power cilia. These complexes are found in some cilia and are assembled in the cytoplasm prior to transport for cilia formation. Mutations in this gene have been associated with primary ciliary dyskinesia. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009].
Disorder MIM:	612517
Disorder Html:	Ciliary dyskinesia, primary, 10, 612518 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001083908.1	NP_001077377.1	protein kintoun isoform 2
NM_018139.2	NP_060609.2	protein kintoun isoform 1

Pathways from BioSystems

Homologies

	LOC719026	XP_001112551.2
Xenopus tropicalis (tropical clawed frog)	dnaaf2	NP_001072474.1
Bos taurus (cattle)	DNAAF2	NP_001069492.2
Pan troglodytes (chimpanzee)	DNAAF2	XP_509930.3
Mus musculus (house mouse)	Dnaaf2	NP_081545.3
Canis lupus familiaris (dog)	DNAAF2	XP_005623972.1
Rattus norvegicus (Norway rat)	Dnaaf2	NP_001014219.2
Homo sapiens (human)	DNAAF2	NP_060609.2
Gallus gallus (chicken)	DNAAF2	XP_421456.4

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in PIH1D3 Cause X-Linked Primary Ciliary Dyskinesia with Outer and Inner Dynein Arm Defects.
Paff T, Loges NT, Aprea I, Wu K, Bakey Z, Haarman EG, Daniels JMA, Sistermans EA, Bogunovic N, Dougherty GW, H?ben IM, Gro?e-Onnebrink J, Matter A, Olbrich H, Werner C, Pals G, Schmidts M, Omran H, Micha D
American journal of human genetics100(1)160-168(2017 Jan)

DYX1C1 is required for axonemal dynein assembly and ciliary motility.
Tarkar A, Loges NT, Slagle CE, Francis R, Dougherty GW, Tamayo JV, Shook B, Cantino M, Schwartz D, Jahnke C, Olbrich H, Werner C, Raidt J, Pennekamp P, Abouhamed M, Hjeij R, K?hler G, Griese M, Li Y, Lemke K, Klena N, Liu X, Gabriel G, Tobita K, Jaspers M, Morgan LC, Shapiro AJ, Letteboer SJ, Mans DA, Carson JL, Leigh MW, Wolf WE, Chen S, Lucas JS, Onoufriadis A, Plagnol V, Schmidts M, Boldt K, , Roepman R, Zariwala MA, Lo CW, Mitchison HM, Knowles MR, Burdine RD, Loturco JJ, Omran H
Nature genetics45(9)995-1003(2013 Sep)

Image-based genome-wide siRNA screen identifies selective autophagy factors.
Orvedahl A, Sumpter R Jr, Xiao G, Ng A, Zou Z, Tang Y, Narimatsu M, Gilpin C, Sun Q, Roth M, Forst CV, Wrana JL, Zhang YE, Luby-Phelps K, Xavier RJ, Xie Y, Levine B
Nature480(7375)113-7(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ktu/PF13 is one of the long-sought proteins involved in pre-assembly of dynein arm complexes in the cytoplasm before intraflagellar transport loads them for the ciliary compartment.
Title: Ktu/PF13 is required for cytoplasmic pre-assembly of axonemal dyneins.

The following DNAAF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DNAAF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu18551	NM_001083908.1 Latest version!	Homo sapiens dynein axonemal assembly factor 2 (DNAAF2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu19388	NM_018139.2 Latest version!	Homo sapiens dynein axonemal assembly factor 2 (DNAAF2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$594.30 $849.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DNAAF2 ( NM_001083908.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001077377.1 Homo sapiens dynein axonemal assembly factor 2 (DNAAF2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu18551
Clone ID Related Accession (Same CDS sequence)	NM_001083908.1
Accession Version	NM_001083908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Homo sapiens(human)
Product	protein kintoun isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX248765.1, BC011400.1, AL139099.3 and BX092175.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC011400.1, SRR1163657.121228.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2161836, SAMEA2467143 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAAG CGGCGGCCTC CTCGTCGCTG GAGGACTTGG ACCTGAGCGG AGAGGAGGTC 
CAGCGGCTCA CCTCCGCCTT CCAGGACCCG GAGTTCCGGC GAATGTTCTC CCAGTACGCC 
GAGGAGCTCA CCGACCCGGA GAACCGGCGG CGCTACGAGG CGGAGATCAC CGCGCTAGAG 
CGTGAGCGCG GGGTGGAAGT GCGGTTCGTG CACCCGGAGC CCGGCCATGT GCTGCGCACC 
AGCCTGGACG GGGCGCGGCG CTGCTTTGTG AATGTCTGCA GCAACGCGTT GGTGGGCGCG 
CCCAGCAGCC GGCCCGGCTC CGGTGGCGAC CGGGGCGCAG CTCCTGGCAG CCACTGGTCC 
CTGCCCTACA GCCTGGCGCC CGGCCGCGAG TACGCGGGGC GCAGCAGCAG CCGCTACATG 
GTCTACGACG TGGTCTTCCA TCCAGACGCG CTTGCGCTGG CCCGGCGGCA CGAGGGCTTC 
CGCCAGATGC TGGACGCCAC GGCCCTGGAG GCCGTCGAGA AGCAGTTCGG CGTGAAGCTG 
GACCGCAGGA ATGCCAAGAC CCTGAAGGCC AAGTATAAGG GGACCCCAGA GGCTGCGGTG 
CTGCGCACGC CCCTGCCCGG GGTCATCCCC GCAAGGCCTG ACGGGGAGCC GAAGGGTCCT 
CTCCCGGACT TCCCCTACCC TTACCAGTAC CCGGCAGCCC CCGGGCCCCG GGCGCCCTCC 
CCTCCGGAAG CGGCCTTGCA GCCCGCCCCC ACCGAGCCTC GCTACAGCGT GGTGCAGCGC 
CACCACGTGG ACCTCCAGGA TTACCGCTGC TCCAGGGACT CAGCCCCGAG CCCCGTGCCC 
CATGAGCTGG TGATCACCAT CGAACTGCCG CTGTTGCGCT CGGCCGAGCA GGCGGCGCTG 
GAGGTAACGA GAAAGCTGCT GTGCCTCGAC TCGAGGAAAC CTGACTACCG GCTGCGGCTC 
TCGCTCCCGT ACCCAGTGGA CGATGGCCGC GGCAAGGCAC AATTCAACAA GGCCCGGCGG 
CAGCTGGTGG TTACGCTGCC AGTGGTGCTG CCGGCCGCGC GCCGGGAGCC CGCTGTCGCC 
GTCGCCGCCG CCGCGCCGGA AGAGTCCGCG GACCGGTCCG GAACTGACGG CCAGGCCTGC 
GCTTCCGCTC GCGAGGGGGA GGCGGGACCC GCGAGGAGTC GCGCGGAGGA CGGAGGCCAC 
GATACCTGCG TGGCTGGGGC TGCGGGCTCC GGGGTCACCA CCCTGGGCGA CCCGGAGGTG 
GCGCCTCCGC CGGCCGCAGC TGGAGAGGAG CGTGTCCCCA AGCCGGGGGA GCAGGACTTG 
AGCAGGCACG CGGGGTCACC GCCGGGCAGC GTGGAGGAGC CATCTCCTGG AGGAGAAAAC 
TCACCTGGTG GCGGAGGCTC CCCTTGTTTG TCCTCCCGGA GCCTGGCGTG GGGTTCTTCT 
GCGGGAAGAG AGAGTGCGCG CGGAGATAGC AGTGTGGAAA CACGCGAGGA GTCGGAGGGC 
ACGGGCGGCC AGCGCTCAGC CTGCGCCATG GGTGGTCCCG GGACCAAGAG CGGGGAGCCT 
TTGTGTCCTC CGTTACTGTG TAATCAGGAC AAAGAAACCT TGACTCTGCT CATTCAGGTG 
CCTCGGATCC AGCCGCAAAG TCTTCAAGGA GATTTGAATC CCCTCTGGTA CAAATTACGC 
TTCTCCGCAC AAGACTTAGT TTATTCCTTC TTTTTGCAAT TTGCTCCAGA GAATAAATTG 
AGTACCACAG AACCTGTGAT TAGCATTTCT TCAAACAATG CAGTGATAGA ACTGGCAAAA 
TCTCCAGAGA GCCATGGACA TTGGAGAGAG TGGTATTATG GTGTAAACAA CGATTCTTTG 
GAGTTGCAAG AATGTAGTAA CTCTGATCAG CTACAAGGAA AGGAGGAAAG AGTAAATGAA 
GAAAGTCATC TAACTGAAAA GGAATATATA GAACATTGTA ACACCCCTAC AACTGATTCT 
GATTCATCTA TAGCAGTTAA AGCACTACAA ATAGATAGCT TTGGTTTAGT TACATGCTTT 
CAACAAGAGT CTCTTGATGT TTCTCAAATG ATACTTGGAA AATCTCAGCA ACCTGAGTCA 
AAAATGCAAT CTGAATTTAT AAAAGAAAAA AGTGCTACTT GTTCAAATGA GGAAAAAGAT 
AACTTAAACG AGTCAGTAAT AACTGAAGAG AAAGAAACAG ATGGAGATCA CCTATCTTCA 
TTACTGAACA AAACTACGGT TCACAATATA CCTGGATTCG ACAGCATAAA AGAAACCAAT 
ATGCAGGATG GTAGTGTGCA GGTCATTAAA GATCATGTGA CCAATTGTGC ATTCAGTTTT 
CAGAATTCTT TGCTATATGA TTTGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077377.1
CDS	82..2451
Translation	MAKAAASSSLEDLDLSGEEVQRLTSAFQDPEFRRMFSQYAEELTDPENRRRYEAEITALERERGVEVRFVHPEPGHVLRTSLDGARRCFVNVCSNALVGAPSSRPGSGGDRGAAPGSHWSLPYSLAPGREYAGRSSSRYMVYDVVFHPDALALARRHEGFRQMLDATALEAVEKQFGVKLDRRNAKTLKAKYKGTPEAAVLRTPLPGVIPARPDGEPKGPLPDFPYPYQYPAAPGPRAPSPPEAALQPAPTEPRYSVVQRHHVDLQDYRCSRDSAPSPVPHELVITIELPLLRSAEQAALEVTRKLLCLDSRKPDYRLRLSLPYPVDDGRGKAQFNKARRQLVVTLPVVLPAARREPAVAVAAAAPEESADRSGTDGQACASAREGEAGPARSRAEDGGHDTCVAGAAGSGVTTLGDPEVAPPPAAAGEERVPKPGEQDLSRHAGSPPGSVEEPSPGGENSPGGGGSPCLSSRSLAWGSSAGRESARGDSSVETREESEGTGGQRSACAMGGPGTKSGEPLCPPLLCNQDKETLTLLIQVPRIQPQSLQGDLNPLWYKLRFSAQDLVYSFFLQFAPENKLSTTEPVISISSNNAVIELAKSPESHGHWREWYYGVNNDSLELQECSNSDQLQGKEERVNEESHLTEKEYIEHCNTPTTDSDSSIAVKALQIDSFGLVTCFQQESLDVSQMILGKSQQPESKMQSEFIKEKSATCSNEEKDNLNESVITEEKETDGDHLSSLLNKTTVHNIPGFDSIKETNMQDGSVQVIKDHVTNCAFSFQNSLLYDLD

Target ORF information:

RefSeq Version	NM_001083908.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dynein axonemal assembly factor 2 (DNAAF2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083908.1

ORF Insert Sequence:

ATGGCCAAAG CGGCGGCCTC CTCGTCGCTG GAGGACTTGG ACCTGAGCGG AGAGGAGGTC 
CAGCGGCTCA CCTCCGCCTT CCAGGACCCG GAGTTCCGGC GAATGTTCTC CCAGTACGCC 
GAGGAGCTCA CCGACCCGGA GAACCGGCGG CGCTACGAGG CGGAGATCAC CGCGCTAGAG 
CGTGAGCGCG GGGTGGAAGT GCGGTTCGTG CACCCGGAGC CCGGCCATGT GCTGCGCACC 
AGCCTGGACG GGGCGCGGCG CTGCTTTGTG AATGTCTGCA GCAACGCGTT GGTGGGCGCG 
CCCAGCAGCC GGCCCGGCTC CGGTGGCGAC CGGGGCGCAG CTCCTGGCAG CCACTGGTCC 
CTGCCCTACA GCCTGGCGCC CGGCCGCGAG TACGCGGGGC GCAGCAGCAG CCGCTACATG 
GTCTACGACG TGGTCTTCCA TCCAGACGCG CTTGCGCTGG CCCGGCGGCA CGAGGGCTTC 
CGCCAGATGC TGGACGCCAC GGCCCTGGAG GCCGTCGAGA AGCAGTTCGG CGTGAAGCTG 
GACCGCAGGA ATGCCAAGAC CCTGAAGGCC AAGTATAAGG GGACCCCAGA GGCTGCGGTG 
CTGCGCACGC CCCTGCCCGG GGTCATCCCC GCAAGGCCTG ACGGGGAGCC GAAGGGTCCT 
CTCCCGGACT TCCCCTACCC TTACCAGTAC CCGGCAGCCC CCGGGCCCCG GGCGCCCTCC 
CCTCCGGAAG CGGCCTTGCA GCCCGCCCCC ACCGAGCCTC GCTACAGCGT GGTGCAGCGC 
CACCACGTGG ACCTCCAGGA TTACCGCTGC TCCAGGGACT CAGCCCCGAG CCCCGTGCCC 
CATGAGCTGG TGATCACCAT CGAACTGCCG CTGTTGCGCT CGGCCGAGCA GGCGGCGCTG 
GAGGTAACGA GAAAGCTGCT GTGCCTCGAC TCGAGGAAAC CTGACTACCG GCTGCGGCTC 
TCGCTCCCGT ACCCAGTGGA CGATGGCCGC GGCAAGGCAC AATTCAACAA GGCCCGGCGG 
CAGCTGGTGG TTACGCTGCC AGTGGTGCTG CCGGCCGCGC GCCGGGAGCC CGCTGTCGCC 
GTCGCCGCCG CCGCGCCGGA AGAGTCCGCG GACCGGTCCG GAACTGACGG CCAGGCCTGC 
GCTTCCGCTC GCGAGGGGGA GGCGGGACCC GCGAGGAGTC GCGCGGAGGA CGGAGGCCAC 
GATACCTGCG TGGCTGGGGC TGCGGGCTCC GGGGTCACCA CCCTGGGCGA CCCGGAGGTG 
GCGCCTCCGC CGGCCGCAGC TGGAGAGGAG CGTGTCCCCA AGCCGGGGGA GCAGGACTTG 
AGCAGGCACG CGGGGTCACC GCCGGGCAGC GTGGAGGAGC CATCTCCTGG AGGAGAAAAC 
TCACCTGGTG GCGGAGGCTC CCCTTGTTTG TCCTCCCGGA GCCTGGCGTG GGGTTCTTCT 
GCGGGAAGAG AGAGTGCGCG CGGAGATAGC AGTGTGGAAA CACGCGAGGA GTCGGAGGGC 
ACGGGCGGCC AGCGCTCAGC CTGCGCCATG GGTGGTCCCG GGACCAAGAG CGGGGAGCCT 
TTGTGTCCTC CGTTACTGTG TAATCAGGAC AAAGAAACCT TGACTCTGCT CATTCAGGTG 
CCTCGGATCC AGCCGCAAAG TCTTCAAGGA GATTTGAATC CCCTCTGGTA CAAATTACGC 
TTCTCCGCAC AAGACTTAGT TTATTCCTTC TTTTTGCAAT TTGCTCCAGA GAATAAATTG 
AGTACCACAG AACCTGTGAT TAGCATTTCT TCAAACAATG CAGTGATAGA ACTGGCAAAA 
TCTCCAGAGA GCCATGGACA TTGGAGAGAG TGGTATTATG GTGTAAACAA CGATTCTTTG 
GAGTTGCAAG AATGTAGTAA CTCTGATCAG CTACAAGGAA AGGAGGAAAG AGTAAATGAA 
GAAAGTCATC TAACTGAAAA GGAATATATA GAACATTGTA ACACCCCTAC AACTGATTCT 
GATTCATCTA TAGCAGTTAA AGCACTACAA ATAGATAGCT TTGGTTTAGT TACATGCTTT 
CAACAAGAGT CTCTTGATGT TTCTCAAATG ATACTTGGAA AATCTCAGCA ACCTGAGTCA 
AAAATGCAAT CTGAATTTAT AAAAGAAAAA AGTGCTACTT GTTCAAATGA GGAAAAAGAT 
AACTTAAACG AGTCAGTAAT AACTGAAGAG AAAGAAACAG ATGGAGATCA CCTATCTTCA 
TTACTGAACA AAACTACGGT TCACAATATA CCTGGATTCG ACAGCATAAA AGAAACCAAT 
ATGCAGGATG GTAGTGTGCA GGTCATTAAA GATCATGTGA CCAATTGTGC ATTCAGTTTT 
CAGAATTCTT TGCTATATGA TTTGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNAAF2 ( NM_018139.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_060609.2 Homo sapiens dynein axonemal assembly factor 2 (DNAAF2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19388
Clone ID Related Accession (Same CDS sequence)	NM_018139.2
Accession Version	NM_018139.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	protein kintoun isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX248765.1, AK001425.1, AL139099.3 and BX092175.1. This sequence is a reference standard in the RefSeqGene project. On Apr 20, 2007 this sequence version replaced NM_018139.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1163655.158695.1, FJ158843.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153427, SAMEA2467148 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAAG CGGCGGCCTC CTCGTCGCTG GAGGACTTGG ACCTGAGCGG AGAGGAGGTC 
CAGCGGCTCA CCTCCGCCTT CCAGGACCCG GAGTTCCGGC GAATGTTCTC CCAGTACGCC 
GAGGAGCTCA CCGACCCGGA GAACCGGCGG CGCTACGAGG CGGAGATCAC CGCGCTAGAG 
CGTGAGCGCG GGGTGGAAGT GCGGTTCGTG CACCCGGAGC CCGGCCATGT GCTGCGCACC 
AGCCTGGACG GGGCGCGGCG CTGCTTTGTG AATGTCTGCA GCAACGCGTT GGTGGGCGCG 
CCCAGCAGCC GGCCCGGCTC CGGTGGCGAC CGGGGCGCAG CTCCTGGCAG CCACTGGTCC 
CTGCCCTACA GCCTGGCGCC CGGCCGCGAG TACGCGGGGC GCAGCAGCAG CCGCTACATG 
GTCTACGACG TGGTCTTCCA TCCAGACGCG CTTGCGCTGG CCCGGCGGCA CGAGGGCTTC 
CGCCAGATGC TGGACGCCAC GGCCCTGGAG GCCGTCGAGA AGCAGTTCGG CGTGAAGCTG 
GACCGCAGGA ATGCCAAGAC CCTGAAGGCC AAGTATAAGG GGACCCCAGA GGCTGCGGTG 
CTGCGCACGC CCCTGCCCGG GGTCATCCCC GCAAGGCCTG ACGGGGAGCC GAAGGGTCCT 
CTCCCGGACT TCCCCTACCC TTACCAGTAC CCGGCAGCCC CCGGGCCCCG GGCGCCCTCC 
CCTCCGGAAG CGGCCTTGCA GCCCGCCCCC ACCGAGCCTC GCTACAGCGT GGTGCAGCGC 
CACCACGTGG ACCTCCAGGA TTACCGCTGC TCCAGGGACT CAGCCCCGAG CCCCGTGCCC 
CATGAGCTGG TGATCACCAT CGAACTGCCG CTGTTGCGCT CGGCCGAGCA GGCGGCGCTG 
GAGGTAACGA GAAAGCTGCT GTGCCTCGAC TCGAGGAAAC CTGACTACCG GCTGCGGCTC 
TCGCTCCCGT ACCCAGTGGA CGATGGCCGC GGCAAGGCAC AATTCAACAA GGCCCGGCGG 
CAGCTGGTGG TTACGCTGCC AGTGGTGCTG CCGGCCGCGC GCCGGGAGCC CGCTGTCGCC 
GTCGCCGCCG CCGCGCCGGA AGAGTCCGCG GACCGGTCCG GAACTGACGG CCAGGCCTGC 
GCTTCCGCTC GCGAGGGGGA GGCGGGACCC GCGAGGAGTC GCGCGGAGGA CGGAGGCCAC 
GATACCTGCG TGGCTGGGGC TGCGGGCTCC GGGGTCACCA CCCTGGGCGA CCCGGAGGTG 
GCGCCTCCGC CGGCCGCAGC TGGAGAGGAG CGTGTCCCCA AGCCGGGGGA GCAGGACTTG 
AGCAGGCACG CGGGGTCACC GCCGGGCAGC GTGGAGGAGC CATCTCCTGG AGGAGAAAAC 
TCACCTGGTG GCGGAGGCTC CCCTTGTTTG TCCTCCCGGA GCCTGGCGTG GGGTTCTTCT 
GCGGGAAGAG AGAGTGCGCG CGGAGATAGC AGTGTGGAAA CACGCGAGGA GTCGGAGGGC 
ACGGGCGGCC AGCGCTCAGC CTGCGCCATG GGTGGTCCCG GGACCAAGAG CGGGGAGCCT 
TTGTGTCCTC CGTTACTGTG TAATCAGGAC AAAGAAACCT TGACTCTGCT CATTCAGGTG 
CCTCGGATCC AGCCGCAAAG TCTTCAAGGA GATTTGAATC CCCTCTGGTA CAAATTACGC 
TTCTCCGCAC AAGACTTAGT TTATTCCTTC TTTTTGCAAT TTGCTCCAGA GAATAAATTG 
AGTACCACAG AACCTGTGAT TAGCATTTCT TCAAACAATG CAGTGATAGA ACTGGCAAAA 
TCTCCAGAGA GCCATGGACA TTGGAGAGAG TGGTATTATG GTGTAAACAA CGATTCTTTG 
GAGGAAAGGT TATTTGTCAA TGAAGAAAAT GTTAATGAGT TTCTTGAAGA GGTCCTGAGC 
TCTCCATTCA AACAGTCTAT GTCCTTGACC CCACCATTAA TTGAAGTTCT TCAAGTTACT 
GATAATAAGA TTCAAATTAA TGCAAAGTTG CAAGAATGTA GTAACTCTGA TCAGCTACAA 
GGAAAGGAGG AAAGAGTAAA TGAAGAAAGT CATCTAACTG AAAAGGAATA TATAGAACAT 
TGTAACACCC CTACAACTGA TTCTGATTCA TCTATAGCAG TTAAAGCACT ACAAATAGAT 
AGCTTTGGTT TAGTTACATG CTTTCAACAA GAGTCTCTTG ATGTTTCTCA AATGATACTT 
GGAAAATCTC AGCAACCTGA GTCAAAAATG CAATCTGAAT TTATAAAAGA AAAAAGTGCT 
ACTTGTTCAA ATGAGGAAAA AGATAACTTA AACGAGTCAG TAATAACTGA AGAGAAAGAA 
ACAGATGGAG ATCACCTATC TTCATTACTG AACAAAACTA CGGTTCACAA TATACCTGGA 
TTCGACAGCA TAAAAGAAAC CAATATGCAG GATGGTAGTG TGCAGGTCAT TAAAGATCAT 
GTGACCAATT GTGCATTCAG TTTTCAGAAT TCTTTGCTAT ATGATTTGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060609.2
CDS	82..2595
Translation	MAKAAASSSLEDLDLSGEEVQRLTSAFQDPEFRRMFSQYAEELTDPENRRRYEAEITALERERGVEVRFVHPEPGHVLRTSLDGARRCFVNVCSNALVGAPSSRPGSGGDRGAAPGSHWSLPYSLAPGREYAGRSSSRYMVYDVVFHPDALALARRHEGFRQMLDATALEAVEKQFGVKLDRRNAKTLKAKYKGTPEAAVLRTPLPGVIPARPDGEPKGPLPDFPYPYQYPAAPGPRAPSPPEAALQPAPTEPRYSVVQRHHVDLQDYRCSRDSAPSPVPHELVITIELPLLRSAEQAALEVTRKLLCLDSRKPDYRLRLSLPYPVDDGRGKAQFNKARRQLVVTLPVVLPAARREPAVAVAAAAPEESADRSGTDGQACASAREGEAGPARSRAEDGGHDTCVAGAAGSGVTTLGDPEVAPPPAAAGEERVPKPGEQDLSRHAGSPPGSVEEPSPGGENSPGGGGSPCLSSRSLAWGSSAGRESARGDSSVETREESEGTGGQRSACAMGGPGTKSGEPLCPPLLCNQDKETLTLLIQVPRIQPQSLQGDLNPLWYKLRFSAQDLVYSFFLQFAPENKLSTTEPVISISSNNAVIELAKSPESHGHWREWYYGVNNDSLEERLFVNEENVNEFLEEVLSSPFKQSMSLTPPLIEVLQVTDNKIQINAKLQECSNSDQLQGKEERVNEESHLTEKEYIEHCNTPTTDSDSSIAVKALQIDSFGLVTCFQQESLDVSQMILGKSQQPESKMQSEFIKEKSATCSNEEKDNLNESVITEEKETDGDHLSSLLNKTTVHNIPGFDSIKETNMQDGSVQVIKDHVTNCAFSFQNSLLYDLD

Target ORF information:

RefSeq Version	NM_018139.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dynein axonemal assembly factor 2 (DNAAF2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018139.2

ORF Insert Sequence:

ATGGCCAAAG CGGCGGCCTC CTCGTCGCTG GAGGACTTGG ACCTGAGCGG AGAGGAGGTC 
CAGCGGCTCA CCTCCGCCTT CCAGGACCCG GAGTTCCGGC GAATGTTCTC CCAGTACGCC 
GAGGAGCTCA CCGACCCGGA GAACCGGCGG CGCTACGAGG CGGAGATCAC CGCGCTAGAG 
CGTGAGCGCG GGGTGGAAGT GCGGTTCGTG CACCCGGAGC CCGGCCATGT GCTGCGCACC 
AGCCTGGACG GGGCGCGGCG CTGCTTTGTG AATGTCTGCA GCAACGCGTT GGTGGGCGCG 
CCCAGCAGCC GGCCCGGCTC CGGTGGCGAC CGGGGCGCAG CTCCTGGCAG CCACTGGTCC 
CTGCCCTACA GCCTGGCGCC CGGCCGCGAG TACGCGGGGC GCAGCAGCAG CCGCTACATG 
GTCTACGACG TGGTCTTCCA TCCAGACGCG CTTGCGCTGG CCCGGCGGCA CGAGGGCTTC 
CGCCAGATGC TGGACGCCAC GGCCCTGGAG GCCGTCGAGA AGCAGTTCGG CGTGAAGCTG 
GACCGCAGGA ATGCCAAGAC CCTGAAGGCC AAGTATAAGG GGACCCCAGA GGCTGCGGTG 
CTGCGCACGC CCCTGCCCGG GGTCATCCCC GCAAGGCCTG ACGGGGAGCC GAAGGGTCCT 
CTCCCGGACT TCCCCTACCC TTACCAGTAC CCGGCAGCCC CCGGGCCCCG GGCGCCCTCC 
CCTCCGGAAG CGGCCTTGCA GCCCGCCCCC ACCGAGCCTC GCTACAGCGT GGTGCAGCGC 
CACCACGTGG ACCTCCAGGA TTACCGCTGC TCCAGGGACT CAGCCCCGAG CCCCGTGCCC 
CATGAGCTGG TGATCACCAT CGAACTGCCG CTGTTGCGCT CGGCCGAGCA GGCGGCGCTG 
GAGGTAACGA GAAAGCTGCT GTGCCTCGAC TCGAGGAAAC CTGACTACCG GCTGCGGCTC 
TCGCTCCCGT ACCCAGTGGA CGATGGCCGC GGCAAGGCAC AATTCAACAA GGCCCGGCGG 
CAGCTGGTGG TTACGCTGCC AGTGGTGCTG CCGGCCGCGC GCCGGGAGCC CGCTGTCGCC 
GTCGCCGCCG CCGCGCCGGA AGAGTCCGCG GACCGGTCCG GAACTGACGG CCAGGCCTGC 
GCTTCCGCTC GCGAGGGGGA GGCGGGACCC GCGAGGAGTC GCGCGGAGGA CGGAGGCCAC 
GATACCTGCG TGGCTGGGGC TGCGGGCTCC GGGGTCACCA CCCTGGGCGA CCCGGAGGTG 
GCGCCTCCGC CGGCCGCAGC TGGAGAGGAG CGTGTCCCCA AGCCGGGGGA GCAGGACTTG 
AGCAGGCACG CGGGGTCACC GCCGGGCAGC GTGGAGGAGC CATCTCCTGG AGGAGAAAAC 
TCACCTGGTG GCGGAGGCTC CCCTTGTTTG TCCTCCCGGA GCCTGGCGTG GGGTTCTTCT 
GCGGGAAGAG AGAGTGCGCG CGGAGATAGC AGTGTGGAAA CACGCGAGGA GTCGGAGGGC 
ACGGGCGGCC AGCGCTCAGC CTGCGCCATG GGTGGTCCCG GGACCAAGAG CGGGGAGCCT 
TTGTGTCCTC CGTTACTGTG TAATCAGGAC AAAGAAACCT TGACTCTGCT CATTCAGGTG 
CCTCGGATCC AGCCGCAAAG TCTTCAAGGA GATTTGAATC CCCTCTGGTA CAAATTACGC 
TTCTCCGCAC AAGACTTAGT TTATTCCTTC TTTTTGCAAT TTGCTCCAGA GAATAAATTG 
AGTACCACAG AACCTGTGAT TAGCATTTCT TCAAACAATG CAGTGATAGA ACTGGCAAAA 
TCTCCAGAGA GCCATGGACA TTGGAGAGAG TGGTATTATG GTGTAAACAA CGATTCTTTG 
GAGGAAAGGT TATTTGTCAA TGAAGAAAAT GTTAATGAGT TTCTTGAAGA GGTCCTGAGC 
TCTCCATTCA AACAGTCTAT GTCCTTGACC CCACCATTAA TTGAAGTTCT TCAAGTTACT 
GATAATAAGA TTCAAATTAA TGCAAAGTTG CAAGAATGTA GTAACTCTGA TCAGCTACAA 
GGAAAGGAGG AAAGAGTAAA TGAAGAAAGT CATCTAACTG AAAAGGAATA TATAGAACAT 
TGTAACACCC CTACAACTGA TTCTGATTCA TCTATAGCAG TTAAAGCACT ACAAATAGAT 
AGCTTTGGTT TAGTTACATG CTTTCAACAA GAGTCTCTTG ATGTTTCTCA AATGATACTT 
GGAAAATCTC AGCAACCTGA GTCAAAAATG CAATCTGAAT TTATAAAAGA AAAAAGTGCT 
ACTTGTTCAA ATGAGGAAAA AGATAACTTA AACGAGTCAG TAATAACTGA AGAGAAAGAA 
ACAGATGGAG ATCACCTATC TTCATTACTG AACAAAACTA CGGTTCACAA TATACCTGGA 
TTCGACAGCA TAAAAGAAAC CAATATGCAG GATGGTAGTG TGCAGGTCAT TAAAGATCAT 
GTGACCAATT GTGCATTCAG TTTTCAGAAT TCTTTGCTAT ATGATTTGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations in PIH1D3 Cause X-Linked Primary Ciliary Dyskinesia with Outer and Inner Dynein Arm Defects.
American journal of human genetics100(1)160-168(2017 Jan)
Paff T,Loges NT,Aprea I,Wu K,Bakey Z,Haarman EG,Daniels JMA,Sistermans EA,Bogunovic N,Dougherty GW,H?ben IM,Gro?e-Onnebrink J,Matter A,Olbrich H,Werner C,Pals G,Schmidts M,Omran H,Micha D

DYX1C1 is required for axonemal dynein assembly and ciliary motility.
Nature genetics45(9)995-1003(2013 Sep)
Tarkar A,Loges NT,Slagle CE,Francis R,Dougherty GW,Tamayo JV,Shook B,Cantino M,Schwartz D,Jahnke C,Olbrich H,Werner C,Raidt J,Pennekamp P,Abouhamed M,Hjeij R,K?hler G,Griese M,Li Y,Lemke K,Klena N,Liu X,Gabriel G,Tobita K,Jaspers M,Morgan LC,Shapiro AJ,Letteboer SJ,Mans DA,Carson JL,Leigh MW,Wolf WE,Chen S,Lucas JS,Onoufriadis A,Plagnol V,Schmidts M,Boldt K,,Roepman R,Zariwala MA,Lo CW,Mitchison HM,Knowles MR,Burdine RD,Loturco JJ,Omran H

Image-based genome-wide siRNA screen identifies selective autophagy factors.
Nature480(7375)113-7(2011 Dec)
Orvedahl A,Sumpter R Jr,Xiao G,Ng A,Zou Z,Tang Y,Narimatsu M,Gilpin C,Sun Q,Roth M,Forst CV,Wrana JL,Zhang YE,Luby-Phelps K,Xavier RJ,Xie Y,Levine B

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DNAAF2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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