RFWD3 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_018124.3	NP_060594.3	E3 ubiquitin-protein ligase RFWD3
NM_001370537.1	NP_001357466.1	E3 ubiquitin-protein ligase RFWD3 isoform 3
NM_001370539.1	NP_001357468.1	E3 ubiquitin-protein ligase RFWD3 isoform 3
NM_001370536.1	NP_001357465.1	E3 ubiquitin-protein ligase RFWD3 isoform 2
NM_001370540.1	NP_001357469.1	E3 ubiquitin-protein ligase RFWD3 isoform 3
NM_001370534.1	NP_001357463.1	E3 ubiquitin-protein ligase RFWD3 isoform 1
XM_017023391.1	XP_016878880.1	E3 ubiquitin-protein ligase RFWD3 isoform X1
NM_018124.4	NP_060594.3	E3 ubiquitin-protein ligase RFWD3 isoform 1
XM_011523191.2	XP_011521493.1	E3 ubiquitin-protein ligase RFWD3 isoform X1
XM_006721228.3	XP_006721291.1	E3 ubiquitin-protein ligase RFWD3 isoform X2
XM_011523191.3	XP_011521493.1	E3 ubiquitin-protein ligase RFWD3 isoform X1
XM_005256021.4	XP_005256078.1	E3 ubiquitin-protein ligase RFWD3 isoform X1
XM_017023392.1	XP_016878881.1	E3 ubiquitin-protein ligase RFWD3 isoform X2
NM_001370535.1	NP_001357464.1	E3 ubiquitin-protein ligase RFWD3 isoform 1
NM_001370543.1	NP_001357472.1	E3 ubiquitin-protein ligase RFWD3 isoform 3
XM_005256022.4	XP_005256079.1	E3 ubiquitin-protein ligase RFWD3 isoform X1
NM_001370542.1	NP_001357471.1	E3 ubiquitin-protein ligase RFWD3 isoform 3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02691	NM_018124.4 Latest version!	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu108103	NM_001370543.1 Latest version!	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu108103	NM_001370537.1 Latest version!	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu108103	NM_001370542.1 Latest version!	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu108103	NM_001370540.1 Latest version!	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu108103	NM_001370539.1 Latest version!	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu02691	NM_001370534.1 Latest version!	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu02691	NM_001370535.1 Latest version!	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu49413	NM_001370536.1 Latest version!	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu02691	XM_011523191.3 Latest version!	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu02691	XM_005256021.4 Latest version!	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu02691	XM_017023391.1 Latest version!	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu02691	XM_005256022.4 Latest version!	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu49413	XM_006721228.3 Latest version!	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu49413	XM_017023392.1 Latest version!	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu02691	NM_018124.3	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu02691	XM_011523191.2	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
Hide Old Accession Versions >>

RFWD3 ( NM_018124.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060594.3 Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02691
Clone ID Related Accession (Same CDS sequence)	NM_018124.3 , NM_001370534.1 , XM_017023391.1 , NM_018124.4 , XM_011523191.2 , XM_011523191.3 , XM_005256021.4 , NM_001370535.1 , XM_005256022.4
Accession Version	NM_018124.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RFWD3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP872110.1, BC059371.1, BC002574.2 and BM467919.1. On Nov 22, 2018 this sequence version replaced NM_018124.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.21672.1, SRR1660805.195981.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000361070.9/ ENSP00000354361.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT 
GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG 
ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG 
ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA 
CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG 
ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA 
TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG 
GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT 
TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT 
AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC 
TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA 
GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC 
CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT 
GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC 
CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT 
AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC 
GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT 
TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA 
CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG 
CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG 
GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG 
ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC 
TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC 
CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC 
ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT 
CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC 
AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA 
GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT 
GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG 
AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC 
TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC 
ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA 
ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC 
AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT 
GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA 
CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG 
GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060594.3
CDS	95..2419
Translation	MAHEAMEYDVQVQLNHAEQQPAPAGMASSQGGPALLQPVPADVVSSQGVPSILQPAPAEVISSQATPPLLQPAPQLSVDLTEVEVLGEDTVENINPRTSEQHRQGSDGNHTIPASSLHSMTNFISGLQRLHGMLEFLRPSSSNHSVGPMRTRRRVSASRRARAGGSQRTDSARLRAPLDAYFQVSRTQPDLPATTYDSETRNPVSEELQVSSSSDSDSDSSAEYGGVVDQAEESGAVILEEQLAGVSAEQEVTCIDGGKTLPKQPSPQKSEPLLPSASMDEEEGDTCTICLEQWTNAGDHRLSALRCGHLFGYRCISTWLKGQVRKCPQCNKKARHSDIVVLYARTLRALDTSEQERMKSSLLKEQMLRKQAELESAQCRLQLQVLTDKCTRLQRRVQDLQKLTSHQSQNLQQPRGSQAWVLSCSPSSQGQHKHKYHFQKTFTVSQAGNCRIMAYCDALSCLVISQPSPQASFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSYLRGLLLSASLDNTIKLTSLETNTVVQTYNAGRPVWSCCWCLDEANYIYAGLANGSILVYDVRNTSSHVQELVAQKARCPLVSLSYMPRAASAAFPYGGVLAGTLEDASFWEQKMDFSHWPHVLPLEPGGCIDFQTENSSRHCLVTYRPDKNHTTIRSVLMEMSYRLDDTGNPICSCQPVHTFFGGPTCKLLTKNAIFQSPENDGNILVCTGDEAANSALLWDAASGSLLQDLQTDQPVLDICPFEVNRNSYLATLTEKMVHIYKWE

Target ORF information:

RefSeq Version	NM_018124.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018124.4

ORF Insert Sequence:

ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT 
GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG 
ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG 
ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA 
CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG 
ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA 
TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG 
GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT 
TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT 
AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC 
TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA 
GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC 
CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT 
GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC 
CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT 
AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC 
GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT 
TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA 
CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG 
CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG 
GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG 
ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC 
TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC 
CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC 
ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT 
CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC 
AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA 
GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT 
GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG 
AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC 
TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC 
ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA 
ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC 
AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT 
GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA 
CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG 
GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( NM_001370537.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001357466.1 Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu108103
Clone ID Related Accession (Same CDS sequence)	NM_001370537.1 , NM_001370539.1 , NM_001370540.1 , NM_001370543.1 , NM_001370542.1
Accession Version	NM_001370537.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RFWD3 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109599.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.4859.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGATGAGG AAGAAGGGGA CACTTGTACA ATATGTCTGG AACAGTGGAC CAATGCTGGG 
GACCACCGGC TCTCAGCATT ACGCTGTGGG CATCTCTTTG GGTATAGGTG CATTTCCACG 
TGGCTTAAAG GACAAGTACG AAAATGTCCC CAGTGCAACA AGAAAGCCAG GCACAGTGAC 
ATTGTCGTCC TTTATGCCCG AACCCTGAGA GCTTTGGACA CTAGTGAACA GGAGCGCATG 
AAAAGTTCCC TACTGAAGGA ACAGATGCTA AGGAAACAGG CCGAGTTAGA ATCAGCACAG 
TGCCGACTCC AACTGCAGGT CCTCACTGAT AAGTGCACTA GGCTTCAAAG GCGTGTTCAG 
GACTTGCAAA AACTTACGTC ACATCAAAGT CAGAATTTAC AGCAACCCAG GGGCTCCCAA 
GCATGGGTCC TGAGCTGCTC ACCCTCCAGC CAGGGCCAGC ACAAGCACAA GTACCACTTC 
CAAAAGACCT TCACAGTATC TCAGGCAGGA AACTGCCGGA TCATGGCATA CTGTGATGCT 
CTGAGCTGCC TGGTGATATC ACAGCCTTCT CCTCAGGCCT CTTTTCTTCC AGGCTTTGGT 
GTTAAGATGT TGAGTACTGC CAACATGAAG AGCAGTCAGT ACATTCCGAT GCATGGCAAA 
CAGATCCGTG GACTGGCGTT TAGCAGTTAC CTCAGAGGCT TGCTACTCTC TGCTTCCCTA 
GACAACACTA TTAAACTGAC CAGCCTGGAG ACAAATACCG TGGTCCAGAC TTATAATGCT 
GGACGTCCTG TCTGGAGCTG TTGCTGGTGT CTTGATGAGG CTAACTACAT CTATGCTGGA 
CTGGCCAATG GTTCAATTCT GGTATATGAC GTGCGAAACA CGAGCAGTCA TGTGCAGGAG 
TTAGTAGCTC AGAAAGCCAG ATGCCCACTG GTCTCCCTGT CATACATGCC CAGAGCTGCC 
TCAGCTGCAT TTCCATATGG TGGGGTGCTG GCTGGAACCT TGGAGGATGC TTCATTCTGG 
GAACAGAAAA TGGACTTTTC TCATTGGCCT CATGTGCTGC CCTTGGAGCC AGGGGGCTGC 
ATAGACTTTC AGACAGAGAA CAGCTCCCGG CACTGTCTTG TGACCTACAG GCCTGATAAA 
AATCACACCA CCATACGAAG TGTGCTGATG GAAATGTCCT ACCGACTGGA TGACACTGGA 
AATCCAATCT GCTCCTGCCA GCCTGTACAT ACATTTTTTG GAGGACCTAC TTGCAAACTA 
TTGACCAAAA ATGCCATTTT CCAAAGCCCA GAGAATGATG GCAACATCCT GGTGTGTACT 
GGGGATGAAG CAGCAAATTC TGCCCTGCTG TGGGATGCTG CCAGTGGCTC GTTGCTCCAG 
GACCTACAGA CCGATCAGCC TGTGTTGGAC ATCTGCCCAT TTGAGGTGAA CCGTAACAGC 
TACTTGGCTA CCTTAACAGA GAAGATGGTC CACATCTATA AGTGGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357466.1
CDS	1103..2593
Translation	MDEEEGDTCTICLEQWTNAGDHRLSALRCGHLFGYRCISTWLKGQVRKCPQCNKKARHSDIVVLYARTLRALDTSEQERMKSSLLKEQMLRKQAELESAQCRLQLQVLTDKCTRLQRRVQDLQKLTSHQSQNLQQPRGSQAWVLSCSPSSQGQHKHKYHFQKTFTVSQAGNCRIMAYCDALSCLVISQPSPQASFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSYLRGLLLSASLDNTIKLTSLETNTVVQTYNAGRPVWSCCWCLDEANYIYAGLANGSILVYDVRNTSSHVQELVAQKARCPLVSLSYMPRAASAAFPYGGVLAGTLEDASFWEQKMDFSHWPHVLPLEPGGCIDFQTENSSRHCLVTYRPDKNHTTIRSVLMEMSYRLDDTGNPICSCQPVHTFFGGPTCKLLTKNAIFQSPENDGNILVCTGDEAANSALLWDAASGSLLQDLQTDQPVLDICPFEVNRNSYLATLTEKMVHIYKWE

Target ORF information:

RefSeq Version	NM_001370537.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370537.1

ORF Insert Sequence:

ATGGATGAGG AAGAAGGGGA CACTTGTACA ATATGTCTGG AACAGTGGAC CAATGCTGGG 
GACCACCGGC TCTCAGCATT ACGCTGTGGG CATCTCTTTG GGTATAGGTG CATTTCCACG 
TGGCTTAAAG GACAAGTACG AAAATGTCCC CAGTGCAACA AGAAAGCCAG GCACAGTGAC 
ATTGTCGTCC TTTATGCCCG AACCCTGAGA GCTTTGGACA CTAGTGAACA GGAGCGCATG 
AAAAGTTCCC TACTGAAGGA ACAGATGCTA AGGAAACAGG CCGAGTTAGA ATCAGCACAG 
TGCCGACTCC AACTGCAGGT CCTCACTGAT AAGTGCACTA GGCTTCAAAG GCGTGTTCAG 
GACTTGCAAA AACTTACGTC ACATCAAAGT CAGAATTTAC AGCAACCCAG GGGCTCCCAA 
GCATGGGTCC TGAGCTGCTC ACCCTCCAGC CAGGGCCAGC ACAAGCACAA GTACCACTTC 
CAAAAGACCT TCACAGTATC TCAGGCAGGA AACTGCCGGA TCATGGCATA CTGTGATGCT 
CTGAGCTGCC TGGTGATATC ACAGCCTTCT CCTCAGGCCT CTTTTCTTCC AGGCTTTGGT 
GTTAAGATGT TGAGTACTGC CAACATGAAG AGCAGTCAGT ACATTCCGAT GCATGGCAAA 
CAGATCCGTG GACTGGCGTT TAGCAGTTAC CTCAGAGGCT TGCTACTCTC TGCTTCCCTA 
GACAACACTA TTAAACTGAC CAGCCTGGAG ACAAATACCG TGGTCCAGAC TTATAATGCT 
GGACGTCCTG TCTGGAGCTG TTGCTGGTGT CTTGATGAGG CTAACTACAT CTATGCTGGA 
CTGGCCAATG GTTCAATTCT GGTATATGAC GTGCGAAACA CGAGCAGTCA TGTGCAGGAG 
TTAGTAGCTC AGAAAGCCAG ATGCCCACTG GTCTCCCTGT CATACATGCC CAGAGCTGCC 
TCAGCTGCAT TTCCATATGG TGGGGTGCTG GCTGGAACCT TGGAGGATGC TTCATTCTGG 
GAACAGAAAA TGGACTTTTC TCATTGGCCT CATGTGCTGC CCTTGGAGCC AGGGGGCTGC 
ATAGACTTTC AGACAGAGAA CAGCTCCCGG CACTGTCTTG TGACCTACAG GCCTGATAAA 
AATCACACCA CCATACGAAG TGTGCTGATG GAAATGTCCT ACCGACTGGA TGACACTGGA 
AATCCAATCT GCTCCTGCCA GCCTGTACAT ACATTTTTTG GAGGACCTAC TTGCAAACTA 
TTGACCAAAA ATGCCATTTT CCAAAGCCCA GAGAATGATG GCAACATCCT GGTGTGTACT 
GGGGATGAAG CAGCAAATTC TGCCCTGCTG TGGGATGCTG CCAGTGGCTC GTTGCTCCAG 
GACCTACAGA CCGATCAGCC TGTGTTGGAC ATCTGCCCAT TTGAGGTGAA CCGTAACAGC 
TACTTGGCTA CCTTAACAGA GAAGATGGTC CACATCTATA AGTGGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( NM_001370539.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001357468.1 Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu108103
Clone ID Related Accession (Same CDS sequence)	NM_001370537.1 , NM_001370539.1 , NM_001370540.1 , NM_001370543.1 , NM_001370542.1
Accession Version	NM_001370539.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RFWD3 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109599.4. On May 20, 2019 this sequence version replaced NM_001370541.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.91940.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGATGAGG AAGAAGGGGA CACTTGTACA ATATGTCTGG AACAGTGGAC CAATGCTGGG 
GACCACCGGC TCTCAGCATT ACGCTGTGGG CATCTCTTTG GGTATAGGTG CATTTCCACG 
TGGCTTAAAG GACAAGTACG AAAATGTCCC CAGTGCAACA AGAAAGCCAG GCACAGTGAC 
ATTGTCGTCC TTTATGCCCG AACCCTGAGA GCTTTGGACA CTAGTGAACA GGAGCGCATG 
AAAAGTTCCC TACTGAAGGA ACAGATGCTA AGGAAACAGG CCGAGTTAGA ATCAGCACAG 
TGCCGACTCC AACTGCAGGT CCTCACTGAT AAGTGCACTA GGCTTCAAAG GCGTGTTCAG 
GACTTGCAAA AACTTACGTC ACATCAAAGT CAGAATTTAC AGCAACCCAG GGGCTCCCAA 
GCATGGGTCC TGAGCTGCTC ACCCTCCAGC CAGGGCCAGC ACAAGCACAA GTACCACTTC 
CAAAAGACCT TCACAGTATC TCAGGCAGGA AACTGCCGGA TCATGGCATA CTGTGATGCT 
CTGAGCTGCC TGGTGATATC ACAGCCTTCT CCTCAGGCCT CTTTTCTTCC AGGCTTTGGT 
GTTAAGATGT TGAGTACTGC CAACATGAAG AGCAGTCAGT ACATTCCGAT GCATGGCAAA 
CAGATCCGTG GACTGGCGTT TAGCAGTTAC CTCAGAGGCT TGCTACTCTC TGCTTCCCTA 
GACAACACTA TTAAACTGAC CAGCCTGGAG ACAAATACCG TGGTCCAGAC TTATAATGCT 
GGACGTCCTG TCTGGAGCTG TTGCTGGTGT CTTGATGAGG CTAACTACAT CTATGCTGGA 
CTGGCCAATG GTTCAATTCT GGTATATGAC GTGCGAAACA CGAGCAGTCA TGTGCAGGAG 
TTAGTAGCTC AGAAAGCCAG ATGCCCACTG GTCTCCCTGT CATACATGCC CAGAGCTGCC 
TCAGCTGCAT TTCCATATGG TGGGGTGCTG GCTGGAACCT TGGAGGATGC TTCATTCTGG 
GAACAGAAAA TGGACTTTTC TCATTGGCCT CATGTGCTGC CCTTGGAGCC AGGGGGCTGC 
ATAGACTTTC AGACAGAGAA CAGCTCCCGG CACTGTCTTG TGACCTACAG GCCTGATAAA 
AATCACACCA CCATACGAAG TGTGCTGATG GAAATGTCCT ACCGACTGGA TGACACTGGA 
AATCCAATCT GCTCCTGCCA GCCTGTACAT ACATTTTTTG GAGGACCTAC TTGCAAACTA 
TTGACCAAAA ATGCCATTTT CCAAAGCCCA GAGAATGATG GCAACATCCT GGTGTGTACT 
GGGGATGAAG CAGCAAATTC TGCCCTGCTG TGGGATGCTG CCAGTGGCTC GTTGCTCCAG 
GACCTACAGA CCGATCAGCC TGTGTTGGAC ATCTGCCCAT TTGAGGTGAA CCGTAACAGC 
TACTTGGCTA CCTTAACAGA GAAGATGGTC CACATCTATA AGTGGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357468.1
CDS	726..2216
Translation	MDEEEGDTCTICLEQWTNAGDHRLSALRCGHLFGYRCISTWLKGQVRKCPQCNKKARHSDIVVLYARTLRALDTSEQERMKSSLLKEQMLRKQAELESAQCRLQLQVLTDKCTRLQRRVQDLQKLTSHQSQNLQQPRGSQAWVLSCSPSSQGQHKHKYHFQKTFTVSQAGNCRIMAYCDALSCLVISQPSPQASFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSYLRGLLLSASLDNTIKLTSLETNTVVQTYNAGRPVWSCCWCLDEANYIYAGLANGSILVYDVRNTSSHVQELVAQKARCPLVSLSYMPRAASAAFPYGGVLAGTLEDASFWEQKMDFSHWPHVLPLEPGGCIDFQTENSSRHCLVTYRPDKNHTTIRSVLMEMSYRLDDTGNPICSCQPVHTFFGGPTCKLLTKNAIFQSPENDGNILVCTGDEAANSALLWDAASGSLLQDLQTDQPVLDICPFEVNRNSYLATLTEKMVHIYKWE

Target ORF information:

RefSeq Version	NM_001370539.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370539.1

ORF Insert Sequence:

ATGGATGAGG AAGAAGGGGA CACTTGTACA ATATGTCTGG AACAGTGGAC CAATGCTGGG 
GACCACCGGC TCTCAGCATT ACGCTGTGGG CATCTCTTTG GGTATAGGTG CATTTCCACG 
TGGCTTAAAG GACAAGTACG AAAATGTCCC CAGTGCAACA AGAAAGCCAG GCACAGTGAC 
ATTGTCGTCC TTTATGCCCG AACCCTGAGA GCTTTGGACA CTAGTGAACA GGAGCGCATG 
AAAAGTTCCC TACTGAAGGA ACAGATGCTA AGGAAACAGG CCGAGTTAGA ATCAGCACAG 
TGCCGACTCC AACTGCAGGT CCTCACTGAT AAGTGCACTA GGCTTCAAAG GCGTGTTCAG 
GACTTGCAAA AACTTACGTC ACATCAAAGT CAGAATTTAC AGCAACCCAG GGGCTCCCAA 
GCATGGGTCC TGAGCTGCTC ACCCTCCAGC CAGGGCCAGC ACAAGCACAA GTACCACTTC 
CAAAAGACCT TCACAGTATC TCAGGCAGGA AACTGCCGGA TCATGGCATA CTGTGATGCT 
CTGAGCTGCC TGGTGATATC ACAGCCTTCT CCTCAGGCCT CTTTTCTTCC AGGCTTTGGT 
GTTAAGATGT TGAGTACTGC CAACATGAAG AGCAGTCAGT ACATTCCGAT GCATGGCAAA 
CAGATCCGTG GACTGGCGTT TAGCAGTTAC CTCAGAGGCT TGCTACTCTC TGCTTCCCTA 
GACAACACTA TTAAACTGAC CAGCCTGGAG ACAAATACCG TGGTCCAGAC TTATAATGCT 
GGACGTCCTG TCTGGAGCTG TTGCTGGTGT CTTGATGAGG CTAACTACAT CTATGCTGGA 
CTGGCCAATG GTTCAATTCT GGTATATGAC GTGCGAAACA CGAGCAGTCA TGTGCAGGAG 
TTAGTAGCTC AGAAAGCCAG ATGCCCACTG GTCTCCCTGT CATACATGCC CAGAGCTGCC 
TCAGCTGCAT TTCCATATGG TGGGGTGCTG GCTGGAACCT TGGAGGATGC TTCATTCTGG 
GAACAGAAAA TGGACTTTTC TCATTGGCCT CATGTGCTGC CCTTGGAGCC AGGGGGCTGC 
ATAGACTTTC AGACAGAGAA CAGCTCCCGG CACTGTCTTG TGACCTACAG GCCTGATAAA 
AATCACACCA CCATACGAAG TGTGCTGATG GAAATGTCCT ACCGACTGGA TGACACTGGA 
AATCCAATCT GCTCCTGCCA GCCTGTACAT ACATTTTTTG GAGGACCTAC TTGCAAACTA 
TTGACCAAAA ATGCCATTTT CCAAAGCCCA GAGAATGATG GCAACATCCT GGTGTGTACT 
GGGGATGAAG CAGCAAATTC TGCCCTGCTG TGGGATGCTG CCAGTGGCTC GTTGCTCCAG 
GACCTACAGA CCGATCAGCC TGTGTTGGAC ATCTGCCCAT TTGAGGTGAA CCGTAACAGC 
TACTTGGCTA CCTTAACAGA GAAGATGGTC CACATCTATA AGTGGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( NM_001370540.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001357469.1 Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu108103
Clone ID Related Accession (Same CDS sequence)	NM_001370537.1 , NM_001370539.1 , NM_001370540.1 , NM_001370543.1 , NM_001370542.1
Accession Version	NM_001370540.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RFWD3 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109599.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.19411.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGATGAGG AAGAAGGGGA CACTTGTACA ATATGTCTGG AACAGTGGAC CAATGCTGGG 
GACCACCGGC TCTCAGCATT ACGCTGTGGG CATCTCTTTG GGTATAGGTG CATTTCCACG 
TGGCTTAAAG GACAAGTACG AAAATGTCCC CAGTGCAACA AGAAAGCCAG GCACAGTGAC 
ATTGTCGTCC TTTATGCCCG AACCCTGAGA GCTTTGGACA CTAGTGAACA GGAGCGCATG 
AAAAGTTCCC TACTGAAGGA ACAGATGCTA AGGAAACAGG CCGAGTTAGA ATCAGCACAG 
TGCCGACTCC AACTGCAGGT CCTCACTGAT AAGTGCACTA GGCTTCAAAG GCGTGTTCAG 
GACTTGCAAA AACTTACGTC ACATCAAAGT CAGAATTTAC AGCAACCCAG GGGCTCCCAA 
GCATGGGTCC TGAGCTGCTC ACCCTCCAGC CAGGGCCAGC ACAAGCACAA GTACCACTTC 
CAAAAGACCT TCACAGTATC TCAGGCAGGA AACTGCCGGA TCATGGCATA CTGTGATGCT 
CTGAGCTGCC TGGTGATATC ACAGCCTTCT CCTCAGGCCT CTTTTCTTCC AGGCTTTGGT 
GTTAAGATGT TGAGTACTGC CAACATGAAG AGCAGTCAGT ACATTCCGAT GCATGGCAAA 
CAGATCCGTG GACTGGCGTT TAGCAGTTAC CTCAGAGGCT TGCTACTCTC TGCTTCCCTA 
GACAACACTA TTAAACTGAC CAGCCTGGAG ACAAATACCG TGGTCCAGAC TTATAATGCT 
GGACGTCCTG TCTGGAGCTG TTGCTGGTGT CTTGATGAGG CTAACTACAT CTATGCTGGA 
CTGGCCAATG GTTCAATTCT GGTATATGAC GTGCGAAACA CGAGCAGTCA TGTGCAGGAG 
TTAGTAGCTC AGAAAGCCAG ATGCCCACTG GTCTCCCTGT CATACATGCC CAGAGCTGCC 
TCAGCTGCAT TTCCATATGG TGGGGTGCTG GCTGGAACCT TGGAGGATGC TTCATTCTGG 
GAACAGAAAA TGGACTTTTC TCATTGGCCT CATGTGCTGC CCTTGGAGCC AGGGGGCTGC 
ATAGACTTTC AGACAGAGAA CAGCTCCCGG CACTGTCTTG TGACCTACAG GCCTGATAAA 
AATCACACCA CCATACGAAG TGTGCTGATG GAAATGTCCT ACCGACTGGA TGACACTGGA 
AATCCAATCT GCTCCTGCCA GCCTGTACAT ACATTTTTTG GAGGACCTAC TTGCAAACTA 
TTGACCAAAA ATGCCATTTT CCAAAGCCCA GAGAATGATG GCAACATCCT GGTGTGTACT 
GGGGATGAAG CAGCAAATTC TGCCCTGCTG TGGGATGCTG CCAGTGGCTC GTTGCTCCAG 
GACCTACAGA CCGATCAGCC TGTGTTGGAC ATCTGCCCAT TTGAGGTGAA CCGTAACAGC 
TACTTGGCTA CCTTAACAGA GAAGATGGTC CACATCTATA AGTGGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357469.1
CDS	579..2069
Translation	MDEEEGDTCTICLEQWTNAGDHRLSALRCGHLFGYRCISTWLKGQVRKCPQCNKKARHSDIVVLYARTLRALDTSEQERMKSSLLKEQMLRKQAELESAQCRLQLQVLTDKCTRLQRRVQDLQKLTSHQSQNLQQPRGSQAWVLSCSPSSQGQHKHKYHFQKTFTVSQAGNCRIMAYCDALSCLVISQPSPQASFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSYLRGLLLSASLDNTIKLTSLETNTVVQTYNAGRPVWSCCWCLDEANYIYAGLANGSILVYDVRNTSSHVQELVAQKARCPLVSLSYMPRAASAAFPYGGVLAGTLEDASFWEQKMDFSHWPHVLPLEPGGCIDFQTENSSRHCLVTYRPDKNHTTIRSVLMEMSYRLDDTGNPICSCQPVHTFFGGPTCKLLTKNAIFQSPENDGNILVCTGDEAANSALLWDAASGSLLQDLQTDQPVLDICPFEVNRNSYLATLTEKMVHIYKWE

Target ORF information:

RefSeq Version	NM_001370540.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370540.1

ORF Insert Sequence:

ATGGATGAGG AAGAAGGGGA CACTTGTACA ATATGTCTGG AACAGTGGAC CAATGCTGGG 
GACCACCGGC TCTCAGCATT ACGCTGTGGG CATCTCTTTG GGTATAGGTG CATTTCCACG 
TGGCTTAAAG GACAAGTACG AAAATGTCCC CAGTGCAACA AGAAAGCCAG GCACAGTGAC 
ATTGTCGTCC TTTATGCCCG AACCCTGAGA GCTTTGGACA CTAGTGAACA GGAGCGCATG 
AAAAGTTCCC TACTGAAGGA ACAGATGCTA AGGAAACAGG CCGAGTTAGA ATCAGCACAG 
TGCCGACTCC AACTGCAGGT CCTCACTGAT AAGTGCACTA GGCTTCAAAG GCGTGTTCAG 
GACTTGCAAA AACTTACGTC ACATCAAAGT CAGAATTTAC AGCAACCCAG GGGCTCCCAA 
GCATGGGTCC TGAGCTGCTC ACCCTCCAGC CAGGGCCAGC ACAAGCACAA GTACCACTTC 
CAAAAGACCT TCACAGTATC TCAGGCAGGA AACTGCCGGA TCATGGCATA CTGTGATGCT 
CTGAGCTGCC TGGTGATATC ACAGCCTTCT CCTCAGGCCT CTTTTCTTCC AGGCTTTGGT 
GTTAAGATGT TGAGTACTGC CAACATGAAG AGCAGTCAGT ACATTCCGAT GCATGGCAAA 
CAGATCCGTG GACTGGCGTT TAGCAGTTAC CTCAGAGGCT TGCTACTCTC TGCTTCCCTA 
GACAACACTA TTAAACTGAC CAGCCTGGAG ACAAATACCG TGGTCCAGAC TTATAATGCT 
GGACGTCCTG TCTGGAGCTG TTGCTGGTGT CTTGATGAGG CTAACTACAT CTATGCTGGA 
CTGGCCAATG GTTCAATTCT GGTATATGAC GTGCGAAACA CGAGCAGTCA TGTGCAGGAG 
TTAGTAGCTC AGAAAGCCAG ATGCCCACTG GTCTCCCTGT CATACATGCC CAGAGCTGCC 
TCAGCTGCAT TTCCATATGG TGGGGTGCTG GCTGGAACCT TGGAGGATGC TTCATTCTGG 
GAACAGAAAA TGGACTTTTC TCATTGGCCT CATGTGCTGC CCTTGGAGCC AGGGGGCTGC 
ATAGACTTTC AGACAGAGAA CAGCTCCCGG CACTGTCTTG TGACCTACAG GCCTGATAAA 
AATCACACCA CCATACGAAG TGTGCTGATG GAAATGTCCT ACCGACTGGA TGACACTGGA 
AATCCAATCT GCTCCTGCCA GCCTGTACAT ACATTTTTTG GAGGACCTAC TTGCAAACTA 
TTGACCAAAA ATGCCATTTT CCAAAGCCCA GAGAATGATG GCAACATCCT GGTGTGTACT 
GGGGATGAAG CAGCAAATTC TGCCCTGCTG TGGGATGCTG CCAGTGGCTC GTTGCTCCAG 
GACCTACAGA CCGATCAGCC TGTGTTGGAC ATCTGCCCAT TTGAGGTGAA CCGTAACAGC 
TACTTGGCTA CCTTAACAGA GAAGATGGTC CACATCTATA AGTGGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( NM_001370543.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001357472.1 Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 10, mRNA.

Price & Availability↑

CloneID	OHu108103
Clone ID Related Accession (Same CDS sequence)	NM_001370537.1 , NM_001370539.1 , NM_001370540.1 , NM_001370543.1 , NM_001370542.1
Accession Version	NM_001370543.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RFWD3 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109599.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.234889.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGATGAGG AAGAAGGGGA CACTTGTACA ATATGTCTGG AACAGTGGAC CAATGCTGGG 
GACCACCGGC TCTCAGCATT ACGCTGTGGG CATCTCTTTG GGTATAGGTG CATTTCCACG 
TGGCTTAAAG GACAAGTACG AAAATGTCCC CAGTGCAACA AGAAAGCCAG GCACAGTGAC 
ATTGTCGTCC TTTATGCCCG AACCCTGAGA GCTTTGGACA CTAGTGAACA GGAGCGCATG 
AAAAGTTCCC TACTGAAGGA ACAGATGCTA AGGAAACAGG CCGAGTTAGA ATCAGCACAG 
TGCCGACTCC AACTGCAGGT CCTCACTGAT AAGTGCACTA GGCTTCAAAG GCGTGTTCAG 
GACTTGCAAA AACTTACGTC ACATCAAAGT CAGAATTTAC AGCAACCCAG GGGCTCCCAA 
GCATGGGTCC TGAGCTGCTC ACCCTCCAGC CAGGGCCAGC ACAAGCACAA GTACCACTTC 
CAAAAGACCT TCACAGTATC TCAGGCAGGA AACTGCCGGA TCATGGCATA CTGTGATGCT 
CTGAGCTGCC TGGTGATATC ACAGCCTTCT CCTCAGGCCT CTTTTCTTCC AGGCTTTGGT 
GTTAAGATGT TGAGTACTGC CAACATGAAG AGCAGTCAGT ACATTCCGAT GCATGGCAAA 
CAGATCCGTG GACTGGCGTT TAGCAGTTAC CTCAGAGGCT TGCTACTCTC TGCTTCCCTA 
GACAACACTA TTAAACTGAC CAGCCTGGAG ACAAATACCG TGGTCCAGAC TTATAATGCT 
GGACGTCCTG TCTGGAGCTG TTGCTGGTGT CTTGATGAGG CTAACTACAT CTATGCTGGA 
CTGGCCAATG GTTCAATTCT GGTATATGAC GTGCGAAACA CGAGCAGTCA TGTGCAGGAG 
TTAGTAGCTC AGAAAGCCAG ATGCCCACTG GTCTCCCTGT CATACATGCC CAGAGCTGCC 
TCAGCTGCAT TTCCATATGG TGGGGTGCTG GCTGGAACCT TGGAGGATGC TTCATTCTGG 
GAACAGAAAA TGGACTTTTC TCATTGGCCT CATGTGCTGC CCTTGGAGCC AGGGGGCTGC 
ATAGACTTTC AGACAGAGAA CAGCTCCCGG CACTGTCTTG TGACCTACAG GCCTGATAAA 
AATCACACCA CCATACGAAG TGTGCTGATG GAAATGTCCT ACCGACTGGA TGACACTGGA 
AATCCAATCT GCTCCTGCCA GCCTGTACAT ACATTTTTTG GAGGACCTAC TTGCAAACTA 
TTGACCAAAA ATGCCATTTT CCAAAGCCCA GAGAATGATG GCAACATCCT GGTGTGTACT 
GGGGATGAAG CAGCAAATTC TGCCCTGCTG TGGGATGCTG CCAGTGGCTC GTTGCTCCAG 
GACCTACAGA CCGATCAGCC TGTGTTGGAC ATCTGCCCAT TTGAGGTGAA CCGTAACAGC 
TACTTGGCTA CCTTAACAGA GAAGATGGTC CACATCTATA AGTGGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357472.1
CDS	980..2470
Translation	MDEEEGDTCTICLEQWTNAGDHRLSALRCGHLFGYRCISTWLKGQVRKCPQCNKKARHSDIVVLYARTLRALDTSEQERMKSSLLKEQMLRKQAELESAQCRLQLQVLTDKCTRLQRRVQDLQKLTSHQSQNLQQPRGSQAWVLSCSPSSQGQHKHKYHFQKTFTVSQAGNCRIMAYCDALSCLVISQPSPQASFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSYLRGLLLSASLDNTIKLTSLETNTVVQTYNAGRPVWSCCWCLDEANYIYAGLANGSILVYDVRNTSSHVQELVAQKARCPLVSLSYMPRAASAAFPYGGVLAGTLEDASFWEQKMDFSHWPHVLPLEPGGCIDFQTENSSRHCLVTYRPDKNHTTIRSVLMEMSYRLDDTGNPICSCQPVHTFFGGPTCKLLTKNAIFQSPENDGNILVCTGDEAANSALLWDAASGSLLQDLQTDQPVLDICPFEVNRNSYLATLTEKMVHIYKWE

Target ORF information:

RefSeq Version	NM_001370543.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370543.1

ORF Insert Sequence:

ATGGATGAGG AAGAAGGGGA CACTTGTACA ATATGTCTGG AACAGTGGAC CAATGCTGGG 
GACCACCGGC TCTCAGCATT ACGCTGTGGG CATCTCTTTG GGTATAGGTG CATTTCCACG 
TGGCTTAAAG GACAAGTACG AAAATGTCCC CAGTGCAACA AGAAAGCCAG GCACAGTGAC 
ATTGTCGTCC TTTATGCCCG AACCCTGAGA GCTTTGGACA CTAGTGAACA GGAGCGCATG 
AAAAGTTCCC TACTGAAGGA ACAGATGCTA AGGAAACAGG CCGAGTTAGA ATCAGCACAG 
TGCCGACTCC AACTGCAGGT CCTCACTGAT AAGTGCACTA GGCTTCAAAG GCGTGTTCAG 
GACTTGCAAA AACTTACGTC ACATCAAAGT CAGAATTTAC AGCAACCCAG GGGCTCCCAA 
GCATGGGTCC TGAGCTGCTC ACCCTCCAGC CAGGGCCAGC ACAAGCACAA GTACCACTTC 
CAAAAGACCT TCACAGTATC TCAGGCAGGA AACTGCCGGA TCATGGCATA CTGTGATGCT 
CTGAGCTGCC TGGTGATATC ACAGCCTTCT CCTCAGGCCT CTTTTCTTCC AGGCTTTGGT 
GTTAAGATGT TGAGTACTGC CAACATGAAG AGCAGTCAGT ACATTCCGAT GCATGGCAAA 
CAGATCCGTG GACTGGCGTT TAGCAGTTAC CTCAGAGGCT TGCTACTCTC TGCTTCCCTA 
GACAACACTA TTAAACTGAC CAGCCTGGAG ACAAATACCG TGGTCCAGAC TTATAATGCT 
GGACGTCCTG TCTGGAGCTG TTGCTGGTGT CTTGATGAGG CTAACTACAT CTATGCTGGA 
CTGGCCAATG GTTCAATTCT GGTATATGAC GTGCGAAACA CGAGCAGTCA TGTGCAGGAG 
TTAGTAGCTC AGAAAGCCAG ATGCCCACTG GTCTCCCTGT CATACATGCC CAGAGCTGCC 
TCAGCTGCAT TTCCATATGG TGGGGTGCTG GCTGGAACCT TGGAGGATGC TTCATTCTGG 
GAACAGAAAA TGGACTTTTC TCATTGGCCT CATGTGCTGC CCTTGGAGCC AGGGGGCTGC 
ATAGACTTTC AGACAGAGAA CAGCTCCCGG CACTGTCTTG TGACCTACAG GCCTGATAAA 
AATCACACCA CCATACGAAG TGTGCTGATG GAAATGTCCT ACCGACTGGA TGACACTGGA 
AATCCAATCT GCTCCTGCCA GCCTGTACAT ACATTTTTTG GAGGACCTAC TTGCAAACTA 
TTGACCAAAA ATGCCATTTT CCAAAGCCCA GAGAATGATG GCAACATCCT GGTGTGTACT 
GGGGATGAAG CAGCAAATTC TGCCCTGCTG TGGGATGCTG CCAGTGGCTC GTTGCTCCAG 
GACCTACAGA CCGATCAGCC TGTGTTGGAC ATCTGCCCAT TTGAGGTGAA CCGTAACAGC 
TACTTGGCTA CCTTAACAGA GAAGATGGTC CACATCTATA AGTGGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( NM_001370542.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001357471.1 Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu108103
Clone ID Related Accession (Same CDS sequence)	NM_001370537.1 , NM_001370539.1 , NM_001370540.1 , NM_001370543.1 , NM_001370542.1
Accession Version	NM_001370542.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RFWD3 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109599.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.152782.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGATGAGG AAGAAGGGGA CACTTGTACA ATATGTCTGG AACAGTGGAC CAATGCTGGG 
GACCACCGGC TCTCAGCATT ACGCTGTGGG CATCTCTTTG GGTATAGGTG CATTTCCACG 
TGGCTTAAAG GACAAGTACG AAAATGTCCC CAGTGCAACA AGAAAGCCAG GCACAGTGAC 
ATTGTCGTCC TTTATGCCCG AACCCTGAGA GCTTTGGACA CTAGTGAACA GGAGCGCATG 
AAAAGTTCCC TACTGAAGGA ACAGATGCTA AGGAAACAGG CCGAGTTAGA ATCAGCACAG 
TGCCGACTCC AACTGCAGGT CCTCACTGAT AAGTGCACTA GGCTTCAAAG GCGTGTTCAG 
GACTTGCAAA AACTTACGTC ACATCAAAGT CAGAATTTAC AGCAACCCAG GGGCTCCCAA 
GCATGGGTCC TGAGCTGCTC ACCCTCCAGC CAGGGCCAGC ACAAGCACAA GTACCACTTC 
CAAAAGACCT TCACAGTATC TCAGGCAGGA AACTGCCGGA TCATGGCATA CTGTGATGCT 
CTGAGCTGCC TGGTGATATC ACAGCCTTCT CCTCAGGCCT CTTTTCTTCC AGGCTTTGGT 
GTTAAGATGT TGAGTACTGC CAACATGAAG AGCAGTCAGT ACATTCCGAT GCATGGCAAA 
CAGATCCGTG GACTGGCGTT TAGCAGTTAC CTCAGAGGCT TGCTACTCTC TGCTTCCCTA 
GACAACACTA TTAAACTGAC CAGCCTGGAG ACAAATACCG TGGTCCAGAC TTATAATGCT 
GGACGTCCTG TCTGGAGCTG TTGCTGGTGT CTTGATGAGG CTAACTACAT CTATGCTGGA 
CTGGCCAATG GTTCAATTCT GGTATATGAC GTGCGAAACA CGAGCAGTCA TGTGCAGGAG 
TTAGTAGCTC AGAAAGCCAG ATGCCCACTG GTCTCCCTGT CATACATGCC CAGAGCTGCC 
TCAGCTGCAT TTCCATATGG TGGGGTGCTG GCTGGAACCT TGGAGGATGC TTCATTCTGG 
GAACAGAAAA TGGACTTTTC TCATTGGCCT CATGTGCTGC CCTTGGAGCC AGGGGGCTGC 
ATAGACTTTC AGACAGAGAA CAGCTCCCGG CACTGTCTTG TGACCTACAG GCCTGATAAA 
AATCACACCA CCATACGAAG TGTGCTGATG GAAATGTCCT ACCGACTGGA TGACACTGGA 
AATCCAATCT GCTCCTGCCA GCCTGTACAT ACATTTTTTG GAGGACCTAC TTGCAAACTA 
TTGACCAAAA ATGCCATTTT CCAAAGCCCA GAGAATGATG GCAACATCCT GGTGTGTACT 
GGGGATGAAG CAGCAAATTC TGCCCTGCTG TGGGATGCTG CCAGTGGCTC GTTGCTCCAG 
GACCTACAGA CCGATCAGCC TGTGTTGGAC ATCTGCCCAT TTGAGGTGAA CCGTAACAGC 
TACTTGGCTA CCTTAACAGA GAAGATGGTC CACATCTATA AGTGGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357471.1
CDS	1102..2592
Translation	MDEEEGDTCTICLEQWTNAGDHRLSALRCGHLFGYRCISTWLKGQVRKCPQCNKKARHSDIVVLYARTLRALDTSEQERMKSSLLKEQMLRKQAELESAQCRLQLQVLTDKCTRLQRRVQDLQKLTSHQSQNLQQPRGSQAWVLSCSPSSQGQHKHKYHFQKTFTVSQAGNCRIMAYCDALSCLVISQPSPQASFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSYLRGLLLSASLDNTIKLTSLETNTVVQTYNAGRPVWSCCWCLDEANYIYAGLANGSILVYDVRNTSSHVQELVAQKARCPLVSLSYMPRAASAAFPYGGVLAGTLEDASFWEQKMDFSHWPHVLPLEPGGCIDFQTENSSRHCLVTYRPDKNHTTIRSVLMEMSYRLDDTGNPICSCQPVHTFFGGPTCKLLTKNAIFQSPENDGNILVCTGDEAANSALLWDAASGSLLQDLQTDQPVLDICPFEVNRNSYLATLTEKMVHIYKWE

Target ORF information:

RefSeq Version	NM_001370542.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370542.1

ORF Insert Sequence:

ATGGATGAGG AAGAAGGGGA CACTTGTACA ATATGTCTGG AACAGTGGAC CAATGCTGGG 
GACCACCGGC TCTCAGCATT ACGCTGTGGG CATCTCTTTG GGTATAGGTG CATTTCCACG 
TGGCTTAAAG GACAAGTACG AAAATGTCCC CAGTGCAACA AGAAAGCCAG GCACAGTGAC 
ATTGTCGTCC TTTATGCCCG AACCCTGAGA GCTTTGGACA CTAGTGAACA GGAGCGCATG 
AAAAGTTCCC TACTGAAGGA ACAGATGCTA AGGAAACAGG CCGAGTTAGA ATCAGCACAG 
TGCCGACTCC AACTGCAGGT CCTCACTGAT AAGTGCACTA GGCTTCAAAG GCGTGTTCAG 
GACTTGCAAA AACTTACGTC ACATCAAAGT CAGAATTTAC AGCAACCCAG GGGCTCCCAA 
GCATGGGTCC TGAGCTGCTC ACCCTCCAGC CAGGGCCAGC ACAAGCACAA GTACCACTTC 
CAAAAGACCT TCACAGTATC TCAGGCAGGA AACTGCCGGA TCATGGCATA CTGTGATGCT 
CTGAGCTGCC TGGTGATATC ACAGCCTTCT CCTCAGGCCT CTTTTCTTCC AGGCTTTGGT 
GTTAAGATGT TGAGTACTGC CAACATGAAG AGCAGTCAGT ACATTCCGAT GCATGGCAAA 
CAGATCCGTG GACTGGCGTT TAGCAGTTAC CTCAGAGGCT TGCTACTCTC TGCTTCCCTA 
GACAACACTA TTAAACTGAC CAGCCTGGAG ACAAATACCG TGGTCCAGAC TTATAATGCT 
GGACGTCCTG TCTGGAGCTG TTGCTGGTGT CTTGATGAGG CTAACTACAT CTATGCTGGA 
CTGGCCAATG GTTCAATTCT GGTATATGAC GTGCGAAACA CGAGCAGTCA TGTGCAGGAG 
TTAGTAGCTC AGAAAGCCAG ATGCCCACTG GTCTCCCTGT CATACATGCC CAGAGCTGCC 
TCAGCTGCAT TTCCATATGG TGGGGTGCTG GCTGGAACCT TGGAGGATGC TTCATTCTGG 
GAACAGAAAA TGGACTTTTC TCATTGGCCT CATGTGCTGC CCTTGGAGCC AGGGGGCTGC 
ATAGACTTTC AGACAGAGAA CAGCTCCCGG CACTGTCTTG TGACCTACAG GCCTGATAAA 
AATCACACCA CCATACGAAG TGTGCTGATG GAAATGTCCT ACCGACTGGA TGACACTGGA 
AATCCAATCT GCTCCTGCCA GCCTGTACAT ACATTTTTTG GAGGACCTAC TTGCAAACTA 
TTGACCAAAA ATGCCATTTT CCAAAGCCCA GAGAATGATG GCAACATCCT GGTGTGTACT 
GGGGATGAAG CAGCAAATTC TGCCCTGCTG TGGGATGCTG CCAGTGGCTC GTTGCTCCAG 
GACCTACAGA CCGATCAGCC TGTGTTGGAC ATCTGCCCAT TTGAGGTGAA CCGTAACAGC 
TACTTGGCTA CCTTAACAGA GAAGATGGTC CACATCTATA AGTGGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( NM_018124.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060594.3 Homo sapiens ring finger and WD repeat domain 3 (RFWD3), mRNA.

Price & Availability↑

CloneID	OHu02691
Clone ID Related Accession (Same CDS sequence)	NM_018124.3 , NM_001370534.1 , XM_017023391.1 , NM_018124.4 , XM_011523191.2 , XM_011523191.3 , XM_005256021.4 , NM_001370535.1 , XM_005256022.4
Accession Version	NM_018124.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RFWD3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP872110.1, BC059371.1, BC002574.2 and BM467919.1. On Jul 26, 2005 this sequence version replaced NM_018124.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.21672.1, SRR1660805.195981.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT 
GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG 
ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG 
ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA 
CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG 
ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA 
TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG 
GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT 
TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT 
AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC 
TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA 
GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC 
CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT 
GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC 
CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT 
AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC 
GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT 
TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA 
CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG 
CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG 
GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG 
ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC 
TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC 
CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC 
ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT 
CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC 
AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA 
GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT 
GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG 
AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC 
TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC 
ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA 
ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC 
AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT 
GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA 
CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG 
GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060594.3
CDS	99..2423
Translation	MAHEAMEYDVQVQLNHAEQQPAPAGMASSQGGPALLQPVPADVVSSQGVPSILQPAPAEVISSQATPPLLQPAPQLSVDLTEVEVLGEDTVENINPRTSEQHRQGSDGNHTIPASSLHSMTNFISGLQRLHGMLEFLRPSSSNHSVGPMRTRRRVSASRRARAGGSQRTDSARLRAPLDAYFQVSRTQPDLPATTYDSETRNPVSEELQVSSSSDSDSDSSAEYGGVVDQAEESGAVILEEQLAGVSAEQEVTCIDGGKTLPKQPSPQKSEPLLPSASMDEEEGDTCTICLEQWTNAGDHRLSALRCGHLFGYRCISTWLKGQVRKCPQCNKKARHSDIVVLYARTLRALDTSEQERMKSSLLKEQMLRKQAELESAQCRLQLQVLTDKCTRLQRRVQDLQKLTSHQSQNLQQPRGSQAWVLSCSPSSQGQHKHKYHFQKTFTVSQAGNCRIMAYCDALSCLVISQPSPQASFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSYLRGLLLSASLDNTIKLTSLETNTVVQTYNAGRPVWSCCWCLDEANYIYAGLANGSILVYDVRNTSSHVQELVAQKARCPLVSLSYMPRAASAAFPYGGVLAGTLEDASFWEQKMDFSHWPHVLPLEPGGCIDFQTENSSRHCLVTYRPDKNHTTIRSVLMEMSYRLDDTGNPICSCQPVHTFFGGPTCKLLTKNAIFQSPENDGNILVCTGDEAANSALLWDAASGSLLQDLQTDQPVLDICPFEVNRNSYLATLTEKMVHIYKWE

Target ORF information:

RefSeq Version	NM_018124.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018124.3

ORF Insert Sequence:

ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT 
GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG 
ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG 
ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA 
CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG 
ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA 
TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG 
GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT 
TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT 
AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC 
TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA 
GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC 
CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT 
GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC 
CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT 
AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC 
GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT 
TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA 
CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG 
CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG 
GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG 
ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC 
TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC 
CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC 
ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT 
CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC 
AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA 
GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT 
GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG 
AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC 
TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC 
ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA 
ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC 
AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT 
GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA 
CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG 
GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( NM_001370534.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001357463.1 Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02691
Clone ID Related Accession (Same CDS sequence)	NM_018124.3 , NM_001370534.1 , XM_017023391.1 , NM_018124.4 , XM_011523191.2 , XM_011523191.3 , XM_005256021.4 , NM_001370535.1 , XM_005256022.4
Accession Version	NM_001370534.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RFWD3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109599.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.255154.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT 
GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG 
ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG 
ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA 
CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG 
ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA 
TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG 
GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT 
TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT 
AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC 
TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA 
GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC 
CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT 
GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC 
CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT 
AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC 
GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT 
TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA 
CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG 
CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG 
GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG 
ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC 
TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC 
CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC 
ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT 
CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC 
AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA 
GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT 
GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG 
AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC 
TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC 
ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA 
ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC 
AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT 
GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA 
CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG 
GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357463.1
CDS	39..2363
Translation	MAHEAMEYDVQVQLNHAEQQPAPAGMASSQGGPALLQPVPADVVSSQGVPSILQPAPAEVISSQATPPLLQPAPQLSVDLTEVEVLGEDTVENINPRTSEQHRQGSDGNHTIPASSLHSMTNFISGLQRLHGMLEFLRPSSSNHSVGPMRTRRRVSASRRARAGGSQRTDSARLRAPLDAYFQVSRTQPDLPATTYDSETRNPVSEELQVSSSSDSDSDSSAEYGGVVDQAEESGAVILEEQLAGVSAEQEVTCIDGGKTLPKQPSPQKSEPLLPSASMDEEEGDTCTICLEQWTNAGDHRLSALRCGHLFGYRCISTWLKGQVRKCPQCNKKARHSDIVVLYARTLRALDTSEQERMKSSLLKEQMLRKQAELESAQCRLQLQVLTDKCTRLQRRVQDLQKLTSHQSQNLQQPRGSQAWVLSCSPSSQGQHKHKYHFQKTFTVSQAGNCRIMAYCDALSCLVISQPSPQASFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSYLRGLLLSASLDNTIKLTSLETNTVVQTYNAGRPVWSCCWCLDEANYIYAGLANGSILVYDVRNTSSHVQELVAQKARCPLVSLSYMPRAASAAFPYGGVLAGTLEDASFWEQKMDFSHWPHVLPLEPGGCIDFQTENSSRHCLVTYRPDKNHTTIRSVLMEMSYRLDDTGNPICSCQPVHTFFGGPTCKLLTKNAIFQSPENDGNILVCTGDEAANSALLWDAASGSLLQDLQTDQPVLDICPFEVNRNSYLATLTEKMVHIYKWE

Target ORF information:

RefSeq Version	NM_001370534.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370534.1

ORF Insert Sequence:

ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT 
GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG 
ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG 
ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA 
CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG 
ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA 
TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG 
GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT 
TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT 
AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC 
TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA 
GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC 
CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT 
GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC 
CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT 
AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC 
GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT 
TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA 
CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG 
CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG 
GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG 
ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC 
TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC 
CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC 
ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT 
CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC 
AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA 
GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT 
GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG 
AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC 
TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC 
ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA 
ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC 
AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT 
GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA 
CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG 
GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( NM_001370535.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001357464.1 Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 3, mRNA.

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CloneID	OHu02691
Clone ID Related Accession (Same CDS sequence)	NM_018124.3 , NM_001370534.1 , XM_017023391.1 , NM_018124.4 , XM_011523191.2 , XM_011523191.3 , XM_005256021.4 , NM_001370535.1 , XM_005256022.4
Accession Version	NM_001370535.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RFWD3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109599.4. On May 9, 2019 this sequence version replaced XM_005256021.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.110044.1, SRR1803611.149781.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT 
GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG 
ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG 
ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA 
CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG 
ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA 
TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG 
GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT 
TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT 
AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC 
TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA 
GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC 
CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT 
GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC 
CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT 
AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC 
GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT 
TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA 
CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG 
CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG 
GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG 
ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC 
TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC 
CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC 
ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT 
CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC 
AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA 
GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT 
GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG 
AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC 
TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC 
ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA 
ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC 
AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT 
GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA 
CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG 
GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357464.1
CDS	217..2541
Translation	MAHEAMEYDVQVQLNHAEQQPAPAGMASSQGGPALLQPVPADVVSSQGVPSILQPAPAEVISSQATPPLLQPAPQLSVDLTEVEVLGEDTVENINPRTSEQHRQGSDGNHTIPASSLHSMTNFISGLQRLHGMLEFLRPSSSNHSVGPMRTRRRVSASRRARAGGSQRTDSARLRAPLDAYFQVSRTQPDLPATTYDSETRNPVSEELQVSSSSDSDSDSSAEYGGVVDQAEESGAVILEEQLAGVSAEQEVTCIDGGKTLPKQPSPQKSEPLLPSASMDEEEGDTCTICLEQWTNAGDHRLSALRCGHLFGYRCISTWLKGQVRKCPQCNKKARHSDIVVLYARTLRALDTSEQERMKSSLLKEQMLRKQAELESAQCRLQLQVLTDKCTRLQRRVQDLQKLTSHQSQNLQQPRGSQAWVLSCSPSSQGQHKHKYHFQKTFTVSQAGNCRIMAYCDALSCLVISQPSPQASFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSYLRGLLLSASLDNTIKLTSLETNTVVQTYNAGRPVWSCCWCLDEANYIYAGLANGSILVYDVRNTSSHVQELVAQKARCPLVSLSYMPRAASAAFPYGGVLAGTLEDASFWEQKMDFSHWPHVLPLEPGGCIDFQTENSSRHCLVTYRPDKNHTTIRSVLMEMSYRLDDTGNPICSCQPVHTFFGGPTCKLLTKNAIFQSPENDGNILVCTGDEAANSALLWDAASGSLLQDLQTDQPVLDICPFEVNRNSYLATLTEKMVHIYKWE

Target ORF information:

RefSeq Version	NM_001370535.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370535.1

ORF Insert Sequence:

ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT 
GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG 
ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG 
ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA 
CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG 
ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA 
TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG 
GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT 
TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT 
AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC 
TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA 
GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC 
CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT 
GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC 
CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT 
AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC 
GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT 
TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA 
CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG 
CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG 
GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG 
ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC 
TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC 
CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC 
ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT 
CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC 
AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA 
GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT 
GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG 
AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC 
TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC 
ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA 
ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC 
AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT 
GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA 
CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG 
GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( NM_001370536.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001357465.1 Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu49413
Clone ID Related Accession (Same CDS sequence)	NM_001370536.1 , XM_006721228.3 , XM_017023392.1
Accession Version	NM_001370536.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RFWD3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109599.4. On May 9, 2019 this sequence version replaced XM_017023392.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.31049.1, SRR1803613.79167.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT 
GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG 
ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG 
ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA 
CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG 
ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA 
TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG 
GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT 
TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT 
AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC 
TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA 
GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC 
CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT 
GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC 
CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT 
AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC 
GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT 
TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA 
CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG 
CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG 
GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG 
ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC 
TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC 
CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC 
ACTATTAAAC TGACCAGATG CCCACTGGTC TCCCTGTCAT ACATGCCCAG AGCTGCCTCA 
GCTGCATTTC CATATGGTGG GGTGCTGGCT GGAACCTTGG AGGATGCTTC ATTCTGGGAA 
CAGAAAATGG ACTTTTCTCA TTGGCCTCAT GTGCTGCCCT TGGAGCCAGG GGGCTGCATA 
GACTTTCAGA CAGAGAACAG CTCCCGGCAC TGTCTTGTGA CCTACAGGCC TGATAAAAAT 
CACACCACCA TACGAAGTGT GCTGATGGAA ATGTCCTACC GACTGGATGA CACTGGAAAT 
CCAATCTGCT CCTGCCAGCC TGTACATACA TTTTTTGGAG GACCTACTTG CAAACTATTG 
ACCAAAAATG CCATTTTCCA AAGCCCAGAG AATGATGGCA ACATCCTGGT GTGTACTGGG 
GATGAAGCAG CAAATTCTGC CCTGCTGTGG GATGCTGCCA GTGGCTCGTT GCTCCAGGAC 
CTACAGACCG ATCAGCCTGT GTTGGACATC TGCCCATTTG AGGTGAACCG TAACAGCTAC 
TTGGCTACCT TAACAGAGAA GATGGTCCAC ATCTATAAGT GGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357465.1
CDS	95..2242
Translation	MAHEAMEYDVQVQLNHAEQQPAPAGMASSQGGPALLQPVPADVVSSQGVPSILQPAPAEVISSQATPPLLQPAPQLSVDLTEVEVLGEDTVENINPRTSEQHRQGSDGNHTIPASSLHSMTNFISGLQRLHGMLEFLRPSSSNHSVGPMRTRRRVSASRRARAGGSQRTDSARLRAPLDAYFQVSRTQPDLPATTYDSETRNPVSEELQVSSSSDSDSDSSAEYGGVVDQAEESGAVILEEQLAGVSAEQEVTCIDGGKTLPKQPSPQKSEPLLPSASMDEEEGDTCTICLEQWTNAGDHRLSALRCGHLFGYRCISTWLKGQVRKCPQCNKKARHSDIVVLYARTLRALDTSEQERMKSSLLKEQMLRKQAELESAQCRLQLQVLTDKCTRLQRRVQDLQKLTSHQSQNLQQPRGSQAWVLSCSPSSQGQHKHKYHFQKTFTVSQAGNCRIMAYCDALSCLVISQPSPQASFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSYLRGLLLSASLDNTIKLTRCPLVSLSYMPRAASAAFPYGGVLAGTLEDASFWEQKMDFSHWPHVLPLEPGGCIDFQTENSSRHCLVTYRPDKNHTTIRSVLMEMSYRLDDTGNPICSCQPVHTFFGGPTCKLLTKNAIFQSPENDGNILVCTGDEAANSALLWDAASGSLLQDLQTDQPVLDICPFEVNRNSYLATLTEKMVHIYKWE

Target ORF information:

RefSeq Version	NM_001370536.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370536.1

ORF Insert Sequence:

ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT 
GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG 
ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG 
ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA 
CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG 
ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA 
TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG 
GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT 
TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT 
AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC 
TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA 
GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC 
CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT 
GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC 
CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT 
AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC 
GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT 
TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA 
CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG 
CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG 
GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG 
ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC 
TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC 
CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC 
ACTATTAAAC TGACCAGATG CCCACTGGTC TCCCTGTCAT ACATGCCCAG AGCTGCCTCA 
GCTGCATTTC CATATGGTGG GGTGCTGGCT GGAACCTTGG AGGATGCTTC ATTCTGGGAA 
CAGAAAATGG ACTTTTCTCA TTGGCCTCAT GTGCTGCCCT TGGAGCCAGG GGGCTGCATA 
GACTTTCAGA CAGAGAACAG CTCCCGGCAC TGTCTTGTGA CCTACAGGCC TGATAAAAAT 
CACACCACCA TACGAAGTGT GCTGATGGAA ATGTCCTACC GACTGGATGA CACTGGAAAT 
CCAATCTGCT CCTGCCAGCC TGTACATACA TTTTTTGGAG GACCTACTTG CAAACTATTG 
ACCAAAAATG CCATTTTCCA AAGCCCAGAG AATGATGGCA ACATCCTGGT GTGTACTGGG 
GATGAAGCAG CAAATTCTGC CCTGCTGTGG GATGCTGCCA GTGGCTCGTT GCTCCAGGAC 
CTACAGACCG ATCAGCCTGT GTTGGACATC TGCCCATTTG AGGTGAACCG TAACAGCTAC 
TTGGCTACCT TAACAGAGAA GATGGTCCAC ATCTATAAGT GGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( XM_017023391.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878880.1 Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu02691
Clone ID Related Accession (Same CDS sequence)	NM_018124.3 , NM_001370534.1 , XM_017023391.1 , NM_018124.4 , XM_011523191.2 , XM_011523191.3 , XM_005256021.4 , NM_001370535.1 , XM_005256022.4
Accession Version	XM_017023391.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RFWD3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT 
GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG 
ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG 
ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA 
CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG 
ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA 
TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG 
GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT 
TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT 
AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC 
TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA 
GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC 
CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT 
GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC 
CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT 
AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC 
GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT 
TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA 
CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG 
CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG 
GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG 
ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC 
TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC 
CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC 
ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT 
CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC 
AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA 
GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT 
GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG 
AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC 
TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC 
ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA 
ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC 
AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT 
GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA 
CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG 
GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878880.1
CDS	148..2472
Translation	MAHEAMEYDVQVQLNHAEQQPAPAGMASSQGGPALLQPVPADVVSSQGVPSILQPAPAEVISSQATPPLLQPAPQLSVDLTEVEVLGEDTVENINPRTSEQHRQGSDGNHTIPASSLHSMTNFISGLQRLHGMLEFLRPSSSNHSVGPMRTRRRVSASRRARAGGSQRTDSARLRAPLDAYFQVSRTQPDLPATTYDSETRNPVSEELQVSSSSDSDSDSSAEYGGVVDQAEESGAVILEEQLAGVSAEQEVTCIDGGKTLPKQPSPQKSEPLLPSASMDEEEGDTCTICLEQWTNAGDHRLSALRCGHLFGYRCISTWLKGQVRKCPQCNKKARHSDIVVLYARTLRALDTSEQERMKSSLLKEQMLRKQAELESAQCRLQLQVLTDKCTRLQRRVQDLQKLTSHQSQNLQQPRGSQAWVLSCSPSSQGQHKHKYHFQKTFTVSQAGNCRIMAYCDALSCLVISQPSPQASFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSYLRGLLLSASLDNTIKLTSLETNTVVQTYNAGRPVWSCCWCLDEANYIYAGLANGSILVYDVRNTSSHVQELVAQKARCPLVSLSYMPRAASAAFPYGGVLAGTLEDASFWEQKMDFSHWPHVLPLEPGGCIDFQTENSSRHCLVTYRPDKNHTTIRSVLMEMSYRLDDTGNPICSCQPVHTFFGGPTCKLLTKNAIFQSPENDGNILVCTGDEAANSALLWDAASGSLLQDLQTDQPVLDICPFEVNRNSYLATLTEKMVHIYKWE

Target ORF information:

RefSeq Version	XM_017023391.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023391.1

ORF Insert Sequence:

ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT 
GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG 
ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG 
ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA 
CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG 
ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA 
TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG 
GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT 
TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT 
AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC 
TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA 
GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC 
CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT 
GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC 
CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT 
AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC 
GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT 
TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA 
CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG 
CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG 
GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG 
ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC 
TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC 
CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC 
ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT 
CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC 
AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA 
GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT 
GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG 
AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC 
TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC 
ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA 
ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC 
AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT 
GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA 
CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG 
GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( XM_011523191.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521493.1 Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu02691
Clone ID Related Accession (Same CDS sequence)	NM_018124.3 , NM_001370534.1 , XM_017023391.1 , NM_018124.4 , XM_011523191.2 , XM_011523191.3 , XM_005256021.4 , NM_001370535.1 , XM_005256022.4
Accession Version	XM_011523191.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RFWD3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011523191.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT 
GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG 
ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG 
ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA 
CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG 
ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA 
TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG 
GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT 
TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT 
AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC 
TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA 
GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC 
CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT 
GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC 
CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT 
AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC 
GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT 
TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA 
CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG 
CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG 
GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG 
ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC 
TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC 
CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC 
ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT 
CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC 
AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA 
GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT 
GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG 
AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC 
TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC 
ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA 
ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC 
AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT 
GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA 
CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG 
GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011521493.1
CDS	52..2376
Translation	MAHEAMEYDVQVQLNHAEQQPAPAGMASSQGGPALLQPVPADVVSSQGVPSILQPAPAEVISSQATPPLLQPAPQLSVDLTEVEVLGEDTVENINPRTSEQHRQGSDGNHTIPASSLHSMTNFISGLQRLHGMLEFLRPSSSNHSVGPMRTRRRVSASRRARAGGSQRTDSARLRAPLDAYFQVSRTQPDLPATTYDSETRNPVSEELQVSSSSDSDSDSSAEYGGVVDQAEESGAVILEEQLAGVSAEQEVTCIDGGKTLPKQPSPQKSEPLLPSASMDEEEGDTCTICLEQWTNAGDHRLSALRCGHLFGYRCISTWLKGQVRKCPQCNKKARHSDIVVLYARTLRALDTSEQERMKSSLLKEQMLRKQAELESAQCRLQLQVLTDKCTRLQRRVQDLQKLTSHQSQNLQQPRGSQAWVLSCSPSSQGQHKHKYHFQKTFTVSQAGNCRIMAYCDALSCLVISQPSPQASFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSYLRGLLLSASLDNTIKLTSLETNTVVQTYNAGRPVWSCCWCLDEANYIYAGLANGSILVYDVRNTSSHVQELVAQKARCPLVSLSYMPRAASAAFPYGGVLAGTLEDASFWEQKMDFSHWPHVLPLEPGGCIDFQTENSSRHCLVTYRPDKNHTTIRSVLMEMSYRLDDTGNPICSCQPVHTFFGGPTCKLLTKNAIFQSPENDGNILVCTGDEAANSALLWDAASGSLLQDLQTDQPVLDICPFEVNRNSYLATLTEKMVHIYKWE

Target ORF information:

RefSeq Version	XM_011523191.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011523191.2

ORF Insert Sequence:

ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT 
GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG 
ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG 
ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA 
CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG 
ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA 
TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG 
GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT 
TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT 
AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC 
TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA 
GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC 
CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT 
GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC 
CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT 
AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC 
GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT 
TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA 
CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG 
CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG 
GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG 
ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC 
TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC 
CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC 
ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT 
CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC 
AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA 
GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT 
GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG 
AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC 
TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC 
ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA 
ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC 
AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT 
GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA 
CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG 
GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( XM_011523191.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521493.1 Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu02691
Clone ID Related Accession (Same CDS sequence)	NM_018124.3 , NM_001370534.1 , XM_017023391.1 , NM_018124.4 , XM_011523191.2 , XM_011523191.3 , XM_005256021.4 , NM_001370535.1 , XM_005256022.4
Accession Version	XM_011523191.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RFWD3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011523191.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT 
GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG 
ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG 
ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA 
CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG 
ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA 
TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG 
GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT 
TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT 
AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC 
TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA 
GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC 
CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT 
GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC 
CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT 
AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC 
GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT 
TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA 
CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG 
CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG 
GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG 
ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC 
TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC 
CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC 
ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT 
CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC 
AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA 
GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT 
GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG 
AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC 
TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC 
ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA 
ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC 
AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT 
GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA 
CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG 
GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011521493.1
CDS	64..2388
Translation	MAHEAMEYDVQVQLNHAEQQPAPAGMASSQGGPALLQPVPADVVSSQGVPSILQPAPAEVISSQATPPLLQPAPQLSVDLTEVEVLGEDTVENINPRTSEQHRQGSDGNHTIPASSLHSMTNFISGLQRLHGMLEFLRPSSSNHSVGPMRTRRRVSASRRARAGGSQRTDSARLRAPLDAYFQVSRTQPDLPATTYDSETRNPVSEELQVSSSSDSDSDSSAEYGGVVDQAEESGAVILEEQLAGVSAEQEVTCIDGGKTLPKQPSPQKSEPLLPSASMDEEEGDTCTICLEQWTNAGDHRLSALRCGHLFGYRCISTWLKGQVRKCPQCNKKARHSDIVVLYARTLRALDTSEQERMKSSLLKEQMLRKQAELESAQCRLQLQVLTDKCTRLQRRVQDLQKLTSHQSQNLQQPRGSQAWVLSCSPSSQGQHKHKYHFQKTFTVSQAGNCRIMAYCDALSCLVISQPSPQASFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSYLRGLLLSASLDNTIKLTSLETNTVVQTYNAGRPVWSCCWCLDEANYIYAGLANGSILVYDVRNTSSHVQELVAQKARCPLVSLSYMPRAASAAFPYGGVLAGTLEDASFWEQKMDFSHWPHVLPLEPGGCIDFQTENSSRHCLVTYRPDKNHTTIRSVLMEMSYRLDDTGNPICSCQPVHTFFGGPTCKLLTKNAIFQSPENDGNILVCTGDEAANSALLWDAASGSLLQDLQTDQPVLDICPFEVNRNSYLATLTEKMVHIYKWE

Target ORF information:

RefSeq Version	XM_011523191.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011523191.3

ORF Insert Sequence:

ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT 
GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG 
ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG 
ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA 
CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG 
ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA 
TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG 
GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT 
TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT 
AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC 
TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA 
GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC 
CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT 
GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC 
CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT 
AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC 
GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT 
TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA 
CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG 
CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG 
GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG 
ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC 
TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC 
CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC 
ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT 
CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC 
AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA 
GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT 
GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG 
AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC 
TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC 
ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA 
ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC 
AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT 
GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA 
CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG 
GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( XM_005256021.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005256078.1 Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu02691
Clone ID Related Accession (Same CDS sequence)	NM_018124.3 , NM_001370534.1 , XM_017023391.1 , NM_018124.4 , XM_011523191.2 , XM_011523191.3 , XM_005256021.4 , NM_001370535.1 , XM_005256022.4
Accession Version	XM_005256021.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RFWD3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005256021.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT 
GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG 
ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG 
ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA 
CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG 
ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA 
TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG 
GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT 
TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT 
AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC 
TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA 
GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC 
CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT 
GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC 
CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT 
AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC 
GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT 
TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA 
CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG 
CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG 
GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG 
ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC 
TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC 
CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC 
ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT 
CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC 
AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA 
GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT 
GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG 
AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC 
TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC 
ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA 
ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC 
AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT 
GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA 
CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG 
GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005256078.1
CDS	232..2556
Translation	MAHEAMEYDVQVQLNHAEQQPAPAGMASSQGGPALLQPVPADVVSSQGVPSILQPAPAEVISSQATPPLLQPAPQLSVDLTEVEVLGEDTVENINPRTSEQHRQGSDGNHTIPASSLHSMTNFISGLQRLHGMLEFLRPSSSNHSVGPMRTRRRVSASRRARAGGSQRTDSARLRAPLDAYFQVSRTQPDLPATTYDSETRNPVSEELQVSSSSDSDSDSSAEYGGVVDQAEESGAVILEEQLAGVSAEQEVTCIDGGKTLPKQPSPQKSEPLLPSASMDEEEGDTCTICLEQWTNAGDHRLSALRCGHLFGYRCISTWLKGQVRKCPQCNKKARHSDIVVLYARTLRALDTSEQERMKSSLLKEQMLRKQAELESAQCRLQLQVLTDKCTRLQRRVQDLQKLTSHQSQNLQQPRGSQAWVLSCSPSSQGQHKHKYHFQKTFTVSQAGNCRIMAYCDALSCLVISQPSPQASFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSYLRGLLLSASLDNTIKLTSLETNTVVQTYNAGRPVWSCCWCLDEANYIYAGLANGSILVYDVRNTSSHVQELVAQKARCPLVSLSYMPRAASAAFPYGGVLAGTLEDASFWEQKMDFSHWPHVLPLEPGGCIDFQTENSSRHCLVTYRPDKNHTTIRSVLMEMSYRLDDTGNPICSCQPVHTFFGGPTCKLLTKNAIFQSPENDGNILVCTGDEAANSALLWDAASGSLLQDLQTDQPVLDICPFEVNRNSYLATLTEKMVHIYKWE

Target ORF information:

RefSeq Version	XM_005256021.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005256021.4

ORF Insert Sequence:

ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT 
GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG 
ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG 
ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA 
CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG 
ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA 
TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG 
GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT 
TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT 
AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC 
TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA 
GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC 
CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT 
GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC 
CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT 
AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC 
GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT 
TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA 
CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG 
CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG 
GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG 
ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC 
TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC 
CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC 
ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT 
CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC 
AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA 
GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT 
GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG 
AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC 
TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC 
ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA 
ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC 
AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT 
GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA 
CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG 
GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( XM_005256022.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005256079.1 Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu02691
Clone ID Related Accession (Same CDS sequence)	NM_018124.3 , NM_001370534.1 , XM_017023391.1 , NM_018124.4 , XM_011523191.2 , XM_011523191.3 , XM_005256021.4 , NM_001370535.1 , XM_005256022.4
Accession Version	XM_005256022.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RFWD3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005256022.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT 
GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG 
ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG 
ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA 
CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG 
ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA 
TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG 
GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT 
TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT 
AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC 
TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA 
GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC 
CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT 
GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC 
CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT 
AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC 
GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT 
TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA 
CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG 
CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG 
GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG 
ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC 
TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC 
CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC 
ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT 
CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC 
AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA 
GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT 
GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG 
AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC 
TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC 
ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA 
ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC 
AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT 
GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA 
CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG 
GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005256079.1
CDS	982..3306
Translation	MAHEAMEYDVQVQLNHAEQQPAPAGMASSQGGPALLQPVPADVVSSQGVPSILQPAPAEVISSQATPPLLQPAPQLSVDLTEVEVLGEDTVENINPRTSEQHRQGSDGNHTIPASSLHSMTNFISGLQRLHGMLEFLRPSSSNHSVGPMRTRRRVSASRRARAGGSQRTDSARLRAPLDAYFQVSRTQPDLPATTYDSETRNPVSEELQVSSSSDSDSDSSAEYGGVVDQAEESGAVILEEQLAGVSAEQEVTCIDGGKTLPKQPSPQKSEPLLPSASMDEEEGDTCTICLEQWTNAGDHRLSALRCGHLFGYRCISTWLKGQVRKCPQCNKKARHSDIVVLYARTLRALDTSEQERMKSSLLKEQMLRKQAELESAQCRLQLQVLTDKCTRLQRRVQDLQKLTSHQSQNLQQPRGSQAWVLSCSPSSQGQHKHKYHFQKTFTVSQAGNCRIMAYCDALSCLVISQPSPQASFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSYLRGLLLSASLDNTIKLTSLETNTVVQTYNAGRPVWSCCWCLDEANYIYAGLANGSILVYDVRNTSSHVQELVAQKARCPLVSLSYMPRAASAAFPYGGVLAGTLEDASFWEQKMDFSHWPHVLPLEPGGCIDFQTENSSRHCLVTYRPDKNHTTIRSVLMEMSYRLDDTGNPICSCQPVHTFFGGPTCKLLTKNAIFQSPENDGNILVCTGDEAANSALLWDAASGSLLQDLQTDQPVLDICPFEVNRNSYLATLTEKMVHIYKWE

Target ORF information:

RefSeq Version	XM_005256022.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005256022.4

ORF Insert Sequence:

ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT 
GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG 
ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG 
ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA 
CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG 
ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA 
TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG 
GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT 
TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT 
AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC 
TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA 
GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC 
CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT 
GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC 
CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT 
AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC 
GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT 
TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA 
CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG 
CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG 
GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG 
ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC 
TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC 
CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC 
ACTATTAAAC TGACCAGCCT GGAGACAAAT ACCGTGGTCC AGACTTATAA TGCTGGACGT 
CCTGTCTGGA GCTGTTGCTG GTGTCTTGAT GAGGCTAACT ACATCTATGC TGGACTGGCC 
AATGGTTCAA TTCTGGTATA TGACGTGCGA AACACGAGCA GTCATGTGCA GGAGTTAGTA 
GCTCAGAAAG CCAGATGCCC ACTGGTCTCC CTGTCATACA TGCCCAGAGC TGCCTCAGCT 
GCATTTCCAT ATGGTGGGGT GCTGGCTGGA ACCTTGGAGG ATGCTTCATT CTGGGAACAG 
AAAATGGACT TTTCTCATTG GCCTCATGTG CTGCCCTTGG AGCCAGGGGG CTGCATAGAC 
TTTCAGACAG AGAACAGCTC CCGGCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAC 
ACCACCATAC GAAGTGTGCT GATGGAAATG TCCTACCGAC TGGATGACAC TGGAAATCCA 
ATCTGCTCCT GCCAGCCTGT ACATACATTT TTTGGAGGAC CTACTTGCAA ACTATTGACC 
AAAAATGCCA TTTTCCAAAG CCCAGAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT 
GAAGCAGCAA ATTCTGCCCT GCTGTGGGAT GCTGCCAGTG GCTCGTTGCT CCAGGACCTA 
CAGACCGATC AGCCTGTGTT GGACATCTGC CCATTTGAGG TGAACCGTAA CAGCTACTTG 
GCTACCTTAA CAGAGAAGAT GGTCCACATC TATAAGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( XM_006721228.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006721291.1 Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X3, mRNA.

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CloneID	OHu49413
Clone ID Related Accession (Same CDS sequence)	NM_001370536.1 , XM_006721228.3 , XM_017023392.1
Accession Version	XM_006721228.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RFWD3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006721228.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT 
GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG 
ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG 
ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA 
CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG 
ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA 
TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG 
GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT 
TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT 
AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC 
TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA 
GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC 
CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT 
GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC 
CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT 
AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC 
GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT 
TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA 
CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG 
CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG 
GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG 
ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC 
TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC 
CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC 
ACTATTAAAC TGACCAGATG CCCACTGGTC TCCCTGTCAT ACATGCCCAG AGCTGCCTCA 
GCTGCATTTC CATATGGTGG GGTGCTGGCT GGAACCTTGG AGGATGCTTC ATTCTGGGAA 
CAGAAAATGG ACTTTTCTCA TTGGCCTCAT GTGCTGCCCT TGGAGCCAGG GGGCTGCATA 
GACTTTCAGA CAGAGAACAG CTCCCGGCAC TGTCTTGTGA CCTACAGGCC TGATAAAAAT 
CACACCACCA TACGAAGTGT GCTGATGGAA ATGTCCTACC GACTGGATGA CACTGGAAAT 
CCAATCTGCT CCTGCCAGCC TGTACATACA TTTTTTGGAG GACCTACTTG CAAACTATTG 
ACCAAAAATG CCATTTTCCA AAGCCCAGAG AATGATGGCA ACATCCTGGT GTGTACTGGG 
GATGAAGCAG CAAATTCTGC CCTGCTGTGG GATGCTGCCA GTGGCTCGTT GCTCCAGGAC 
CTACAGACCG ATCAGCCTGT GTTGGACATC TGCCCATTTG AGGTGAACCG TAACAGCTAC 
TTGGCTACCT TAACAGAGAA GATGGTCCAC ATCTATAAGT GGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006721291.1
CDS	232..2379
Translation	MAHEAMEYDVQVQLNHAEQQPAPAGMASSQGGPALLQPVPADVVSSQGVPSILQPAPAEVISSQATPPLLQPAPQLSVDLTEVEVLGEDTVENINPRTSEQHRQGSDGNHTIPASSLHSMTNFISGLQRLHGMLEFLRPSSSNHSVGPMRTRRRVSASRRARAGGSQRTDSARLRAPLDAYFQVSRTQPDLPATTYDSETRNPVSEELQVSSSSDSDSDSSAEYGGVVDQAEESGAVILEEQLAGVSAEQEVTCIDGGKTLPKQPSPQKSEPLLPSASMDEEEGDTCTICLEQWTNAGDHRLSALRCGHLFGYRCISTWLKGQVRKCPQCNKKARHSDIVVLYARTLRALDTSEQERMKSSLLKEQMLRKQAELESAQCRLQLQVLTDKCTRLQRRVQDLQKLTSHQSQNLQQPRGSQAWVLSCSPSSQGQHKHKYHFQKTFTVSQAGNCRIMAYCDALSCLVISQPSPQASFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSYLRGLLLSASLDNTIKLTRCPLVSLSYMPRAASAAFPYGGVLAGTLEDASFWEQKMDFSHWPHVLPLEPGGCIDFQTENSSRHCLVTYRPDKNHTTIRSVLMEMSYRLDDTGNPICSCQPVHTFFGGPTCKLLTKNAIFQSPENDGNILVCTGDEAANSALLWDAASGSLLQDLQTDQPVLDICPFEVNRNSYLATLTEKMVHIYKWE

Target ORF information:

RefSeq Version	XM_006721228.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006721228.3

ORF Insert Sequence:

ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT 
GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG 
ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG 
ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA 
CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG 
ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA 
TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG 
GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT 
TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT 
AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC 
TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA 
GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC 
CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT 
GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC 
CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT 
AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC 
GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT 
TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA 
CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG 
CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG 
GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG 
ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC 
TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC 
CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC 
ACTATTAAAC TGACCAGATG CCCACTGGTC TCCCTGTCAT ACATGCCCAG AGCTGCCTCA 
GCTGCATTTC CATATGGTGG GGTGCTGGCT GGAACCTTGG AGGATGCTTC ATTCTGGGAA 
CAGAAAATGG ACTTTTCTCA TTGGCCTCAT GTGCTGCCCT TGGAGCCAGG GGGCTGCATA 
GACTTTCAGA CAGAGAACAG CTCCCGGCAC TGTCTTGTGA CCTACAGGCC TGATAAAAAT 
CACACCACCA TACGAAGTGT GCTGATGGAA ATGTCCTACC GACTGGATGA CACTGGAAAT 
CCAATCTGCT CCTGCCAGCC TGTACATACA TTTTTTGGAG GACCTACTTG CAAACTATTG 
ACCAAAAATG CCATTTTCCA AAGCCCAGAG AATGATGGCA ACATCCTGGT GTGTACTGGG 
GATGAAGCAG CAAATTCTGC CCTGCTGTGG GATGCTGCCA GTGGCTCGTT GCTCCAGGAC 
CTACAGACCG ATCAGCCTGT GTTGGACATC TGCCCATTTG AGGTGAACCG TAACAGCTAC 
TTGGCTACCT TAACAGAGAA GATGGTCCAC ATCTATAAGT GGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( XM_017023392.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878881.1 Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu49413
Clone ID Related Accession (Same CDS sequence)	NM_001370536.1 , XM_006721228.3 , XM_017023392.1
Accession Version	XM_017023392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RFWD3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT 
GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG 
ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG 
ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA 
CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG 
ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA 
TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG 
GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT 
TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT 
AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC 
TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA 
GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC 
CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT 
GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC 
CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT 
AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC 
GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT 
TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA 
CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG 
CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG 
GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG 
ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC 
TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC 
CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC 
ACTATTAAAC TGACCAGATG CCCACTGGTC TCCCTGTCAT ACATGCCCAG AGCTGCCTCA 
GCTGCATTTC CATATGGTGG GGTGCTGGCT GGAACCTTGG AGGATGCTTC ATTCTGGGAA 
CAGAAAATGG ACTTTTCTCA TTGGCCTCAT GTGCTGCCCT TGGAGCCAGG GGGCTGCATA 
GACTTTCAGA CAGAGAACAG CTCCCGGCAC TGTCTTGTGA CCTACAGGCC TGATAAAAAT 
CACACCACCA TACGAAGTGT GCTGATGGAA ATGTCCTACC GACTGGATGA CACTGGAAAT 
CCAATCTGCT CCTGCCAGCC TGTACATACA TTTTTTGGAG GACCTACTTG CAAACTATTG 
ACCAAAAATG CCATTTTCCA AAGCCCAGAG AATGATGGCA ACATCCTGGT GTGTACTGGG 
GATGAAGCAG CAAATTCTGC CCTGCTGTGG GATGCTGCCA GTGGCTCGTT GCTCCAGGAC 
CTACAGACCG ATCAGCCTGT GTTGGACATC TGCCCATTTG AGGTGAACCG TAACAGCTAC 
TTGGCTACCT TAACAGAGAA GATGGTCCAC ATCTATAAGT GGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878881.1
CDS	96..2243
Translation	MAHEAMEYDVQVQLNHAEQQPAPAGMASSQGGPALLQPVPADVVSSQGVPSILQPAPAEVISSQATPPLLQPAPQLSVDLTEVEVLGEDTVENINPRTSEQHRQGSDGNHTIPASSLHSMTNFISGLQRLHGMLEFLRPSSSNHSVGPMRTRRRVSASRRARAGGSQRTDSARLRAPLDAYFQVSRTQPDLPATTYDSETRNPVSEELQVSSSSDSDSDSSAEYGGVVDQAEESGAVILEEQLAGVSAEQEVTCIDGGKTLPKQPSPQKSEPLLPSASMDEEEGDTCTICLEQWTNAGDHRLSALRCGHLFGYRCISTWLKGQVRKCPQCNKKARHSDIVVLYARTLRALDTSEQERMKSSLLKEQMLRKQAELESAQCRLQLQVLTDKCTRLQRRVQDLQKLTSHQSQNLQQPRGSQAWVLSCSPSSQGQHKHKYHFQKTFTVSQAGNCRIMAYCDALSCLVISQPSPQASFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSYLRGLLLSASLDNTIKLTRCPLVSLSYMPRAASAAFPYGGVLAGTLEDASFWEQKMDFSHWPHVLPLEPGGCIDFQTENSSRHCLVTYRPDKNHTTIRSVLMEMSYRLDDTGNPICSCQPVHTFFGGPTCKLLTKNAIFQSPENDGNILVCTGDEAANSALLWDAASGSLLQDLQTDQPVLDICPFEVNRNSYLATLTEKMVHIYKWE

Target ORF information:

RefSeq Version	XM_017023392.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger and WD repeat domain 3 (RFWD3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023392.1

ORF Insert Sequence:

ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC CGAACAACAG 
CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA GCCTGTTCCT 
GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC TGCTGAGGTG 
ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC TGTTGACCTG 
ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG AACTTCAGAA 
CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT GCATTCAATG 
ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT GAGACCTTCA 
TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC TTCACGGAGG 
GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC ATTGGATGCT 
TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA TTCAGAGACT 
AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA CAGTGACAGC 
TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT CATTTTAGAA 
GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG AGGCAAGACC 
CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC TTCTATGGAT 
GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC TGGGGACCAC 
CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGTGCATTTC CACGTGGCTT 
AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG TGACATTGTC 
GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG CATGAAAAGT 
TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC ACAGTGCCGA 
CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAGGCTTC AAAGGCGTGT TCAGGACTTG 
CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC CCAAGCATGG 
GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA CTTCCAAAAG 
ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA TGCTCTGAGC 
TGCCTGGTGA TATCACAGCC TTCTCCTCAG GCCTCTTTTC TTCCAGGCTT TGGTGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CGATGCATGG CAAACAGATC 
CGTGGACTGG CGTTTAGCAG TTACCTCAGA GGCTTGCTAC TCTCTGCTTC CCTAGACAAC 
ACTATTAAAC TGACCAGATG CCCACTGGTC TCCCTGTCAT ACATGCCCAG AGCTGCCTCA 
GCTGCATTTC CATATGGTGG GGTGCTGGCT GGAACCTTGG AGGATGCTTC ATTCTGGGAA 
CAGAAAATGG ACTTTTCTCA TTGGCCTCAT GTGCTGCCCT TGGAGCCAGG GGGCTGCATA 
GACTTTCAGA CAGAGAACAG CTCCCGGCAC TGTCTTGTGA CCTACAGGCC TGATAAAAAT 
CACACCACCA TACGAAGTGT GCTGATGGAA ATGTCCTACC GACTGGATGA CACTGGAAAT 
CCAATCTGCT CCTGCCAGCC TGTACATACA TTTTTTGGAG GACCTACTTG CAAACTATTG 
ACCAAAAATG CCATTTTCCA AAGCCCAGAG AATGATGGCA ACATCCTGGT GTGTACTGGG 
GATGAAGCAG CAAATTCTGC CCTGCTGTGG GATGCTGCCA GTGGCTCGTT GCTCCAGGAC 
CTACAGACCG ATCAGCCTGT GTTGGACATC TGCCCATTTG AGGTGAACCG TAACAGCTAC 
TTGGCTACCT TAACAGAGAA GATGGTCCAC ATCTATAAGT GGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RFWD3
Entrez Gene ID	55159
Full Name	ring finger and WD repeat domain 3
Synonyms	FANCW,RNF201
General protein information	Preferred Names ring finger and WD repeat domain 3 Names E3 ubiquitin-protein ligase RFWD3 RING finger and WD repeat domain-containing protein 3 RING finger protein 201 RING-type E3 ubiquitin transferase RFWD3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q23.1
Disorder MIM:	614151
Disorder Html:	?Fanconi anemia, complementation group W, 617784 (3)

Rattus norvegicus (Norway rat)	Rfwd3	XP_006255701.1
	RFWD3	XP_002802615.1
Danio rerio (zebrafish)	LOC100329327	XP_002667008.1
Drosophila melanogaster (fruit fly)	CG13025	NP_001261971.1
Homo sapiens (human)	RFWD3	NP_060594.3
Mus musculus (house mouse)	Rfwd3	NP_666330.2
Pan troglodytes (chimpanzee)	RFWD3	XP_001137409.1
Canis lupus familiaris (dog)	RFWD3	XP_853024.2
Bos taurus (cattle)	RFWD3	XP_005218437.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005643	XP_315661.2
Xenopus tropicalis (tropical clawed frog)	rfwd3	XP_002931710.1
Gallus gallus (chicken)	RFWD3	XP_414051.3

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RFWD3 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

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