DARS2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DARS2
Entrez Gene ID	55157
Full Name	aspartyl-tRNA synthetase 2, mitochondrial
Synonyms	ASPRS,LBSL,MT-ASPRS
General protein information	Preferred Names aspartyl-tRNA synthetase 2, mitochondrial Names aspartate--tRNA ligase, mitochondrial aspartate tRNA ligase 2, mitochondrial aspartyl-tRNA synthetase, mitochondrial
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q25.1
Summary	The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). [provided by RefSeq, Nov 2009].
Disorder MIM:	610956
Disorder Html:	Leukoencephalopathy with brain stem and spinal cord involvement and lactate elevation, 611105 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006711427.3	XP_006711490.1	aspartate--tRNA ligase, mitochondrial isoform X1
NM_001365213.2	NP_001352142.1	aspartate--tRNA ligase, mitochondrial isoform 3
NM_018122.5	NP_060592.2	aspartate--tRNA ligase, mitochondrial isoform 1
NM_018122.4	NP_060592.2	aspartate--tRNA ligase, mitochondrial
XM_011509711.2	XP_011508013.1	aspartate--tRNA ligase, mitochondrial isoform X2
NM_001365213.1	NP_001352142.1	aspartate--tRNA ligase, mitochondrial isoform 3
NM_001365212.1	NP_001352141.1	aspartate--tRNA ligase, mitochondrial isoform 2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	DARS2	XP_422251.3
Drosophila melanogaster (fruit fly)	CG31739	NP_001260519.1
Schizosaccharomyces pombe (fission yeast)	SPCC736.06	NP_587777.1
Xenopus tropicalis (tropical clawed frog)	dars2	XP_002931512.2
Homo sapiens (human)	DARS2	NP_060592.2
Pan troglodytes (chimpanzee)	DARS2	XP_524974.2
	DARS2	XP_001101931.2
Danio rerio (zebrafish)	dars2	XP_699804.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007844	XP_317655.3
Caenorhabditis elegans (roundworm)	dars-2	NP_506019.2
Kluyveromyces lactis	KLLA0E24465g	XP_455053.1
Eremothecium gossypii	AGOS_AGR206C	NP_986872.1
Neurospora crassa	NCU02585	XP_965716.2
Canis lupus familiaris (dog)	DARS2	XP_547457.2
Magnaporthe oryzae (rice blast fungus)	MGG_13512	XP_003716295.1
Oryza sativa (rice)	Os01g0153400	NP_001042054.1
Bos taurus (cattle)	DARS2	NP_001095692.1
Mus musculus (house mouse)	Dars2	NP_766232.1
Rattus norvegicus (Norway rat)	Dars2	NP_001029315.1
Saccharomyces cerevisiae (baker's yeast)	MSD1	NP_015221.1
Arabidopsis thaliana (thale cress)	AT4G33760	NP_195102.2

Gene Ontology
Bibliography

Related articles in PubMed

Upregulation of DARS2 by HBV promotes hepatocarcinogenesis through the miR-30e-5p/MAPK/NFAT5 pathway.
Qin X, Li C, Guo T, Chen J, Wang HT, Wang YT, Xiao YS, Li J, Liu P, Liu ZS, Liu QY
Journal of experimental & clinical cancer research : CR36(1)148(2017 Oct)

Neurodegenerative disease-associated mutants of a human mitochondrial aminoacyl-tRNA synthetase present individual molecular signatures.
Sauter C, Lorber B, Gaudry A, Karim L, Schwenzer H, Wien F, Roblin P, Florentz C, Sissler M
Scientific reports517332(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutations with mild effects on solubility occur in patients as allelic combinations whereas those with strong effects on solubility or on aminoacylation are necessarily associated with a partially functional allele.
Title: Neurodegenerative disease-associated mutants of a human mitochondrial aminoacyl-tRNA synthetase present individual molecular signatures.

This study identified DARS2-associated leukoencephalopathy with hypomyelination with brainstem and spinal cord involvement and leg spasticity.
Title: DARS-associated leukoencephalopathy can mimic a steroid-responsive neuroinflammatory disorder.

60 different DARS2 mutations were identified in 78 patients with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation, 13 of which have not been reported before
Title: Leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation: clinical and genetic characterization and target for therapy.

Cognitive impairment seems to be common among patients with leukoencephalopathy with brainstem and spinal cord involvement and elevated lactate and DARS2 mutations
Title: Impaired information-processing speed and working memory in leukoencephalopathy with brainstem and spinal cord involvement and elevated lactate (LBSL) and DARS2 mutations: a report of three adult patients.

A comparison of biophysical properties of human mitochondrial aspartyl-tRNA synthetase, HsaDRS2, with them to those of a bacterial (E. coli) homolog, EcoDRS.
Title: Thermodynamic properties distinguish human mitochondrial aspartyl-tRNA synthetase from bacterial homolog with same 3D architecture.

The following DARS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DARS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27194	NM_018122.5 Latest version!	Homo sapiens aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu34519	NM_001365212.1 Latest version!	Homo sapiens aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu69095	NM_001365213.2 Latest version!	Homo sapiens aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu34519	XM_006711427.3 Latest version!	Homo sapiens aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu69095	XM_011509711.2 Latest version!	Homo sapiens aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu27194	NM_018122.4	Homo sapiens aspartyl-tRNA synthetase 2, mitochondrial (DARS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu69095	NM_001365213.1	Homo sapiens aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DARS2 ( NM_018122.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060592.2 Homo sapiens aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27194
Clone ID Related Accession (Same CDS sequence)	NM_018122.5 , NM_018122.4
Accession Version	NM_018122.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	aspartate--tRNA ligase, mitochondrial isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL519850.3, BC045173.2, AK022754.1 and AL109921.21. This sequence is a reference standard in the RefSeqGene project. On Aug 7, 2018 this sequence version replaced NM_018122.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.201082.1, SRR1660809.181326.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000649689.2/ ENSP00000497569.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTACTTCC CTTCTTGGTT AAGTCAGCTG TACAGGGGTT TATCCAGACC CATCAGAAGG 
ACCACCCAAC CGATCTGGGG TTCTCTCTAC AGAAGTCTGT TGCAGAGTTC ACAGAGGAGA 
ATTCCAGAAT TCAGTAGCTT TGTTGTCCGG ACCAACACAT GTGGAGAGTT GCGTTCGTCT 
CACTTAGGCC AAGAAGTCAC CTTGTGTGGA TGGATTCAGT ACCGAAGGCA AAACACATTC 
TTGGTCCTAA GAGATTTCGA TGGGCTTGTT CAAGTTATCA TTCCCCAGGA TGAGTCGGCA 
GCCTCTGTGA AGAAGATTTT ATGTGAAGCC CCTGTGGAAT CTGTGGTGCA AGTGTCTGGT 
ACAGTCATTT CCCGTCCTGC AGGACAAGAG AATCCAAAAA TGCCAACAGG TGAGATTGAA 
ATCAAAGTTA AAACAGCTGA GCTTCTGAAT GCCTGCAAGA AGCTGCCCTT TGAAATTAAG 
AACTTCGTGA AGAAAACAGA GGCTCTTCGG TTGCAGTATC GCTACTTAGA CTTGCGTAGT 
TTCCAAATGC AGTATAACCT GCGACTGAGG TCCCAGATGG TCATGAAAAT GCGGGAATAT 
CTCTGTAATC TGCATGGGTT TGTGGATATA GAAACCCCCA CATTGTTTAA GAGGACCCCA 
GGGGGTGCCA AAGAGTTTTT AGTACCATCC AGGGAACCTG GAAAGTTTTA TTCTCTCCCT 
CAGAGTCCTC AACAGTTTAA GCAACTTCTG ATGGTTGGCG GTTTAGACAG ATATTTTCAG 
GTTGCCCGAT GTTATCGAGA TGAAGGTTCA AGACCAGACA GACAGCCTGA GTTTACTCAG 
ATTGACATAG AGATGTCATT TGTAGACCAG ACTGGGATCC AGAGTTTAAT TGAGGGTTTG 
CTCCAGTATT CCTGGCCCAA TGACAAAGAT CCTGTGGTTG TTCCTTTTCC TACTATGACT 
TTTGCTGAGG TGCTGGCCAC CTATGGAACT GATAAACCTG ACACTCGCTT TGGAATGAAG 
ATTATAGATA TCAGTGATGT GTTTAGAAAC ACAGAGATTG GATTTCTTCA AGATGCACTT 
AGTAAGCCCC ATGGAACTGT GAAAGCCATA TGTATCCCTG AAGGAGCAAA ATACTTAAAA 
AGGAAAGACA TTGAATCCAT TAGAAACTTT GCAGCTGACC ATTTTAATCA GGAAATCTTA 
CCTGTATTCC TTAACGCCAA TAGAAACTGG AATTCTCCAG TTGCTAATTT CATAATGGAG 
TCACAAAGAC TGGAATTAAT CAGACTAATG GAGACCCAAG AGGAAGATGT GGTCCTACTA 
ACTGCTGGAG AGCACAATAA AGCATGCTCT TTGTTAGGAA AATTACGACT GGAATGTGCT 
GACCTTCTAG AAACAAGAGG AGTGGTGCTC CGTGACCCCA CTCTGTTCTC TTTCCTTTGG 
GTGGTAGATT TCCCACTCTT CCTGCCCAAG GAGGAAAATC CCAGAGAGCT GGAATCGGCC 
CACCACCCAT TTACTGCTCC CCACCCCAGT GACATACATC TCCTGTACAC TGAGCCCAAA 
AAGGCCCGTA GCCAACACTA TGACTTGGTT TTAAATGGCA ATGAAATAGG AGGTGGTTCA 
ATTCGAATTC ACAATGCAGA GCTGCAGCGT TATATCCTGG CAACCTTACT AAAGGAGGAT 
GTGAAAATGC TCTCCCATCT GCTCCAGGCT TTAGATTATG GGGCACCCCC TCATGGAGGA 
ATTGCCTTAG GGTTAGACAG ACTGATATGC CTTGTCACTG GATCTCCAAG CATCAGAGAT 
GTCATAGCCT TCCCAAAGTC CTTCCGGGGA CATGACCTCA TGAGCAATAC CCCAGATTCT 
GTCCCTCCTG AGGAACTGAA GCCCTATCAT ATCCGAGTCT CCAAGCCAAC AGACTCCAAA 
GCAGAAAGAG CTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060592.2
CDS	558..2495
Translation	MYFPSWLSQLYRGLSRPIRRTTQPIWGSLYRSLLQSSQRRIPEFSSFVVRTNTCGELRSSHLGQEVTLCGWIQYRRQNTFLVLRDFDGLVQVIIPQDESAASVKKILCEAPVESVVQVSGTVISRPAGQENPKMPTGEIEIKVKTAELLNACKKLPFEIKNFVKKTEALRLQYRYLDLRSFQMQYNLRLRSQMVMKMREYLCNLHGFVDIETPTLFKRTPGGAKEFLVPSREPGKFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQPEFTQIDIEMSFVDQTGIQSLIEGLLQYSWPNDKDPVVVPFPTMTFAEVLATYGTDKPDTRFGMKIIDISDVFRNTEIGFLQDALSKPHGTVKAICIPEGAKYLKRKDIESIRNFAADHFNQEILPVFLNANRNWNSPVANFIMESQRLELIRLMETQEEDVVLLTAGEHNKACSLLGKLRLECADLLETRGVVLRDPTLFSFLWVVDFPLFLPKEENPRELESAHHPFTAPHPSDIHLLYTEPKKARSQHYDLVLNGNEIGGGSIRIHNAELQRYILATLLKEDVKMLSHLLQALDYGAPPHGGIALGLDRLICLVTGSPSIRDVIAFPKSFRGHDLMSNTPDSVPPEELKPYHIRVSKPTDSKAERAH

Target ORF information:

RefSeq Version	NM_018122.5
Organism	Homo sapiens(human)
Definition	Homo sapiens aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018122.5

ORF Insert Sequence:

ATGTACTTCC CTTCTTGGTT AAGTCAGCTG TACAGGGGTT TATCCAGACC CATCAGAAGG 
ACCACCCAAC CGATCTGGGG TTCTCTCTAC AGAAGTCTGT TGCAGAGTTC ACAGAGGAGA 
ATTCCAGAAT TCAGTAGCTT TGTTGTCCGG ACCAACACAT GTGGAGAGTT GCGTTCGTCT 
CACTTAGGCC AAGAAGTCAC CTTGTGTGGA TGGATTCAGT ACCGAAGGCA AAACACATTC 
TTGGTCCTAA GAGATTTCGA TGGGCTTGTT CAAGTTATCA TTCCCCAGGA TGAGTCGGCA 
GCCTCTGTGA AGAAGATTTT ATGTGAAGCC CCTGTGGAAT CTGTGGTGCA AGTGTCTGGT 
ACAGTCATTT CCCGTCCTGC AGGACAAGAG AATCCAAAAA TGCCAACAGG TGAGATTGAA 
ATCAAAGTTA AAACAGCTGA GCTTCTGAAT GCCTGCAAGA AGCTGCCCTT TGAAATTAAG 
AACTTCGTGA AGAAAACAGA GGCTCTTCGG TTGCAGTATC GCTACTTAGA CTTGCGTAGT 
TTCCAAATGC AGTATAACCT GCGACTGAGG TCCCAGATGG TCATGAAAAT GCGGGAATAT 
CTCTGTAATC TGCATGGGTT TGTGGATATA GAAACCCCCA CATTGTTTAA GAGGACCCCA 
GGGGGTGCCA AAGAGTTTTT AGTACCATCC AGGGAACCTG GAAAGTTTTA TTCTCTCCCT 
CAGAGTCCTC AACAGTTTAA GCAACTTCTG ATGGTTGGCG GTTTAGACAG ATATTTTCAG 
GTTGCCCGAT GTTATCGAGA TGAAGGTTCA AGACCAGACA GACAGCCTGA GTTTACTCAG 
ATTGACATAG AGATGTCATT TGTAGACCAG ACTGGGATCC AGAGTTTAAT TGAGGGTTTG 
CTCCAGTATT CCTGGCCCAA TGACAAAGAT CCTGTGGTTG TTCCTTTTCC TACTATGACT 
TTTGCTGAGG TGCTGGCCAC CTATGGAACT GATAAACCTG ACACTCGCTT TGGAATGAAG 
ATTATAGATA TCAGTGATGT GTTTAGAAAC ACAGAGATTG GATTTCTTCA AGATGCACTT 
AGTAAGCCCC ATGGAACTGT GAAAGCCATA TGTATCCCTG AAGGAGCAAA ATACTTAAAA 
AGGAAAGACA TTGAATCCAT TAGAAACTTT GCAGCTGACC ATTTTAATCA GGAAATCTTA 
CCTGTATTCC TTAACGCCAA TAGAAACTGG AATTCTCCAG TTGCTAATTT CATAATGGAG 
TCACAAAGAC TGGAATTAAT CAGACTAATG GAGACCCAAG AGGAAGATGT GGTCCTACTA 
ACTGCTGGAG AGCACAATAA AGCATGCTCT TTGTTAGGAA AATTACGACT GGAATGTGCT 
GACCTTCTAG AAACAAGAGG AGTGGTGCTC CGTGACCCCA CTCTGTTCTC TTTCCTTTGG 
GTGGTAGATT TCCCACTCTT CCTGCCCAAG GAGGAAAATC CCAGAGAGCT GGAATCGGCC 
CACCACCCAT TTACTGCTCC CCACCCCAGT GACATACATC TCCTGTACAC TGAGCCCAAA 
AAGGCCCGTA GCCAACACTA TGACTTGGTT TTAAATGGCA ATGAAATAGG AGGTGGTTCA 
ATTCGAATTC ACAATGCAGA GCTGCAGCGT TATATCCTGG CAACCTTACT AAAGGAGGAT 
GTGAAAATGC TCTCCCATCT GCTCCAGGCT TTAGATTATG GGGCACCCCC TCATGGAGGA 
ATTGCCTTAG GGTTAGACAG ACTGATATGC CTTGTCACTG GATCTCCAAG CATCAGAGAT 
GTCATAGCCT TCCCAAAGTC CTTCCGGGGA CATGACCTCA TGAGCAATAC CCCAGATTCT 
GTCCCTCCTG AGGAACTGAA GCCCTATCAT ATCCGAGTCT CCAAGCCAAC AGACTCCAAA 
GCAGAAAGAG CTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DARS2 ( NM_018122.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060592.2 Homo sapiens aspartyl-tRNA synthetase 2, mitochondrial (DARS2), mRNA.

Price & Availability↑

CloneID	OHu27194
Clone ID Related Accession (Same CDS sequence)	NM_018122.5 , NM_018122.4
Accession Version	NM_018122.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	aspartate--tRNA ligase, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL519850.3, BC045173.2 and AK022754.1. This sequence is a reference standard in the RefSeqGene project. On Nov 13, 2009 this sequence version replaced NM_018122.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.197992.1, SRR1660809.176393.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTACTTCC CTTCTTGGTT AAGTCAGCTG TACAGGGGTT TATCCAGACC CATCAGAAGG 
ACCACCCAAC CGATCTGGGG TTCTCTCTAC AGAAGTCTGT TGCAGAGTTC ACAGAGGAGA 
ATTCCAGAAT TCAGTAGCTT TGTTGTCCGG ACCAACACAT GTGGAGAGTT GCGTTCGTCT 
CACTTAGGCC AAGAAGTCAC CTTGTGTGGA TGGATTCAGT ACCGAAGGCA AAACACATTC 
TTGGTCCTAA GAGATTTCGA TGGGCTTGTT CAAGTTATCA TTCCCCAGGA TGAGTCGGCA 
GCCTCTGTGA AGAAGATTTT ATGTGAAGCC CCTGTGGAAT CTGTGGTGCA AGTGTCTGGT 
ACAGTCATTT CCCGTCCTGC AGGACAAGAG AATCCAAAAA TGCCAACAGG TGAGATTGAA 
ATCAAAGTTA AAACAGCTGA GCTTCTGAAT GCCTGCAAGA AGCTGCCCTT TGAAATTAAG 
AACTTCGTGA AGAAAACAGA GGCTCTTCGG TTGCAGTATC GCTACTTAGA CTTGCGTAGT 
TTCCAAATGC AGTATAACCT GCGACTGAGG TCCCAGATGG TCATGAAAAT GCGGGAATAT 
CTCTGTAATC TGCATGGGTT TGTGGATATA GAAACCCCCA CATTGTTTAA GAGGACCCCA 
GGGGGTGCCA AAGAGTTTTT AGTACCATCC AGGGAACCTG GAAAGTTTTA TTCTCTCCCT 
CAGAGTCCTC AACAGTTTAA GCAACTTCTG ATGGTTGGCG GTTTAGACAG ATATTTTCAG 
GTTGCCCGAT GTTATCGAGA TGAAGGTTCA AGACCAGACA GACAGCCTGA GTTTACTCAG 
ATTGACATAG AGATGTCATT TGTAGACCAG ACTGGGATCC AGAGTTTAAT TGAGGGTTTG 
CTCCAGTATT CCTGGCCCAA TGACAAAGAT CCTGTGGTTG TTCCTTTTCC TACTATGACT 
TTTGCTGAGG TGCTGGCCAC CTATGGAACT GATAAACCTG ACACTCGCTT TGGAATGAAG 
ATTATAGATA TCAGTGATGT GTTTAGAAAC ACAGAGATTG GATTTCTTCA AGATGCACTT 
AGTAAGCCCC ATGGAACTGT GAAAGCCATA TGTATCCCTG AAGGAGCAAA ATACTTAAAA 
AGGAAAGACA TTGAATCCAT TAGAAACTTT GCAGCTGACC ATTTTAATCA GGAAATCTTA 
CCTGTATTCC TTAACGCCAA TAGAAACTGG AATTCTCCAG TTGCTAATTT CATAATGGAG 
TCACAAAGAC TGGAATTAAT CAGACTAATG GAGACCCAAG AGGAAGATGT GGTCCTACTA 
ACTGCTGGAG AGCACAATAA AGCATGCTCT TTGTTAGGAA AATTACGACT GGAATGTGCT 
GACCTTCTAG AAACAAGAGG AGTGGTGCTC CGTGACCCCA CTCTGTTCTC TTTCCTTTGG 
GTGGTAGATT TCCCACTCTT CCTGCCCAAG GAGGAAAATC CCAGAGAGCT GGAATCGGCC 
CACCACCCAT TTACTGCTCC CCACCCCAGT GACATACATC TCCTGTACAC TGAGCCCAAA 
AAGGCCCGTA GCCAACACTA TGACTTGGTT TTAAATGGCA ATGAAATAGG AGGTGGTTCA 
ATTCGAATTC ACAATGCAGA GCTGCAGCGT TATATCCTGG CAACCTTACT AAAGGAGGAT 
GTGAAAATGC TCTCCCATCT GCTCCAGGCT TTAGATTATG GGGCACCCCC TCATGGAGGA 
ATTGCCTTAG GGTTAGACAG ACTGATATGC CTTGTCACTG GATCTCCAAG CATCAGAGAT 
GTCATAGCCT TCCCAAAGTC CTTCCGGGGA CATGACCTCA TGAGCAATAC CCCAGATTCT 
GTCCCTCCTG AGGAACTGAA GCCCTATCAT ATCCGAGTCT CCAAGCCAAC AGACTCCAAA 
GCAGAAAGAG CTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060592.2
CDS	572..2509
Translation	MYFPSWLSQLYRGLSRPIRRTTQPIWGSLYRSLLQSSQRRIPEFSSFVVRTNTCGELRSSHLGQEVTLCGWIQYRRQNTFLVLRDFDGLVQVIIPQDESAASVKKILCEAPVESVVQVSGTVISRPAGQENPKMPTGEIEIKVKTAELLNACKKLPFEIKNFVKKTEALRLQYRYLDLRSFQMQYNLRLRSQMVMKMREYLCNLHGFVDIETPTLFKRTPGGAKEFLVPSREPGKFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQPEFTQIDIEMSFVDQTGIQSLIEGLLQYSWPNDKDPVVVPFPTMTFAEVLATYGTDKPDTRFGMKIIDISDVFRNTEIGFLQDALSKPHGTVKAICIPEGAKYLKRKDIESIRNFAADHFNQEILPVFLNANRNWNSPVANFIMESQRLELIRLMETQEEDVVLLTAGEHNKACSLLGKLRLECADLLETRGVVLRDPTLFSFLWVVDFPLFLPKEENPRELESAHHPFTAPHPSDIHLLYTEPKKARSQHYDLVLNGNEIGGGSIRIHNAELQRYILATLLKEDVKMLSHLLQALDYGAPPHGGIALGLDRLICLVTGSPSIRDVIAFPKSFRGHDLMSNTPDSVPPEELKPYHIRVSKPTDSKAERAH

Target ORF information:

RefSeq Version	NM_018122.4
Organism	Homo sapiens(human)
Definition	Homo sapiens aspartyl-tRNA synthetase 2, mitochondrial (DARS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018122.4

ORF Insert Sequence:

ATGTACTTCC CTTCTTGGTT AAGTCAGCTG TACAGGGGTT TATCCAGACC CATCAGAAGG 
ACCACCCAAC CGATCTGGGG TTCTCTCTAC AGAAGTCTGT TGCAGAGTTC ACAGAGGAGA 
ATTCCAGAAT TCAGTAGCTT TGTTGTCCGG ACCAACACAT GTGGAGAGTT GCGTTCGTCT 
CACTTAGGCC AAGAAGTCAC CTTGTGTGGA TGGATTCAGT ACCGAAGGCA AAACACATTC 
TTGGTCCTAA GAGATTTCGA TGGGCTTGTT CAAGTTATCA TTCCCCAGGA TGAGTCGGCA 
GCCTCTGTGA AGAAGATTTT ATGTGAAGCC CCTGTGGAAT CTGTGGTGCA AGTGTCTGGT 
ACAGTCATTT CCCGTCCTGC AGGACAAGAG AATCCAAAAA TGCCAACAGG TGAGATTGAA 
ATCAAAGTTA AAACAGCTGA GCTTCTGAAT GCCTGCAAGA AGCTGCCCTT TGAAATTAAG 
AACTTCGTGA AGAAAACAGA GGCTCTTCGG TTGCAGTATC GCTACTTAGA CTTGCGTAGT 
TTCCAAATGC AGTATAACCT GCGACTGAGG TCCCAGATGG TCATGAAAAT GCGGGAATAT 
CTCTGTAATC TGCATGGGTT TGTGGATATA GAAACCCCCA CATTGTTTAA GAGGACCCCA 
GGGGGTGCCA AAGAGTTTTT AGTACCATCC AGGGAACCTG GAAAGTTTTA TTCTCTCCCT 
CAGAGTCCTC AACAGTTTAA GCAACTTCTG ATGGTTGGCG GTTTAGACAG ATATTTTCAG 
GTTGCCCGAT GTTATCGAGA TGAAGGTTCA AGACCAGACA GACAGCCTGA GTTTACTCAG 
ATTGACATAG AGATGTCATT TGTAGACCAG ACTGGGATCC AGAGTTTAAT TGAGGGTTTG 
CTCCAGTATT CCTGGCCCAA TGACAAAGAT CCTGTGGTTG TTCCTTTTCC TACTATGACT 
TTTGCTGAGG TGCTGGCCAC CTATGGAACT GATAAACCTG ACACTCGCTT TGGAATGAAG 
ATTATAGATA TCAGTGATGT GTTTAGAAAC ACAGAGATTG GATTTCTTCA AGATGCACTT 
AGTAAGCCCC ATGGAACTGT GAAAGCCATA TGTATCCCTG AAGGAGCAAA ATACTTAAAA 
AGGAAAGACA TTGAATCCAT TAGAAACTTT GCAGCTGACC ATTTTAATCA GGAAATCTTA 
CCTGTATTCC TTAACGCCAA TAGAAACTGG AATTCTCCAG TTGCTAATTT CATAATGGAG 
TCACAAAGAC TGGAATTAAT CAGACTAATG GAGACCCAAG AGGAAGATGT GGTCCTACTA 
ACTGCTGGAG AGCACAATAA AGCATGCTCT TTGTTAGGAA AATTACGACT GGAATGTGCT 
GACCTTCTAG AAACAAGAGG AGTGGTGCTC CGTGACCCCA CTCTGTTCTC TTTCCTTTGG 
GTGGTAGATT TCCCACTCTT CCTGCCCAAG GAGGAAAATC CCAGAGAGCT GGAATCGGCC 
CACCACCCAT TTACTGCTCC CCACCCCAGT GACATACATC TCCTGTACAC TGAGCCCAAA 
AAGGCCCGTA GCCAACACTA TGACTTGGTT TTAAATGGCA ATGAAATAGG AGGTGGTTCA 
ATTCGAATTC ACAATGCAGA GCTGCAGCGT TATATCCTGG CAACCTTACT AAAGGAGGAT 
GTGAAAATGC TCTCCCATCT GCTCCAGGCT TTAGATTATG GGGCACCCCC TCATGGAGGA 
ATTGCCTTAG GGTTAGACAG ACTGATATGC CTTGTCACTG GATCTCCAAG CATCAGAGAT 
GTCATAGCCT TCCCAAAGTC CTTCCGGGGA CATGACCTCA TGAGCAATAC CCCAGATTCT 
GTCCCTCCTG AGGAACTGAA GCCCTATCAT ATCCGAGTCT CCAAGCCAAC AGACTCCAAA 
GCAGAAAGAG CTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DARS2 ( NM_001365212.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001352141.1 Homo sapiens aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu34519
Clone ID Related Accession (Same CDS sequence)	XM_006711427.3 , NM_001365212.1
Accession Version	NM_001365212.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	aspartate--tRNA ligase, mitochondrial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL109921.21. On Aug 7, 2018 this sequence version replaced XM_006711427.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.226068.1, SRR1803616.88977.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTACTTCC CTTCTTGGTT AAGTCAGCTG TACAGGGGTT TATCCAGACC CATCAGAAGG 
ACCACCCAAC CGATCTGGGG TTCTCTCTAC AGAAGTCTGT TGCAGAGTTC ACAGAGGAGA 
ATTCCAGAAT TCAGTAGCTT TGTTGTCCGG ACCAACACAT GTGGAGAGTT GCGTTCGTCT 
CACTTAGGCC AAGAAGTCAC CTTGTGTGGA TGGATTCAGT ACCGAAGGCA AAACACATTC 
TTGGTCCTAA GAGATTTCGA TGGGCTTGTT CAAGTTATCA TTCCCCAGGA TGAGTCGGCA 
GCCTCTGTGA AGAAGATTTT ATGTGAAGCC CCTGTGGAAT CTGTGGTGCA AGTGTCTGGT 
ACAGTCATTT CCCGTCCTGC AGGACAAGAG AATCCAAAAA TGCCAACAGG TGAGATTGAA 
ATCAAAGTTA AAACAGCTGA GCTTCTGAAT GCCTGCAAGA AGCTGCCCTT TGAAATTAAG 
AACTTCGTGA AGAAAACAGA GGCTCTTCGG TTGCAGTATC GCTACTTAGA CTTGCGTAGT 
TTCCAAATGC AGTATAACCT GCGACTGAGG TCCCAGATGG TCATGAAAAT GCGGGAATAT 
CTCTGTAATC TGCATGGGTT TGTGGATATA GAAACCCCCA CATTGTTTAA GAGGACCCCA 
GGGGGTGCCA AAGAGTTTTT AGTACCATCC AGGGAACCTG GAAAGTTTTA TTCTCTCCCT 
CAGAGTCCTC AACAGTTTAA GCAACTTCTG ATGGTTGGCG GTTTAGACAG ATATTTTCAG 
GTTGCCCGAT GTTATCGAGA TGAAGGTTCA AGACCAGACA GACAGCCTGA GTTTACTCAG 
ATTGACATAG AGATGTCATT TGTAGACCAG ACTGGGATCC AGAGTTTAAT TGAGGGTTTG 
CTCCAGTATT CCTGGCCCAA TGACAAAGAT CCTGTGGTTG TTCCTTTTCC TACTATGACT 
TTTGCTGAGG TGCTGGCCAC CTATGGAACT GATAAACCTG ACACTCGCTT TGGAATGAAG 
ATTATAGATA TCAGTGATGT GTTTAGAAAC ACAGAGATTG GATTTCTTCA AGATGCACTT 
AGTAAGCCCC ATGGAACTGT GAAAGCCATA TGTATCCCTG AAGGAGCAAA ATACTTAAAA 
AGGAAAGACA TTGAATCCAT TAGAAACTTT GCAGCTGACC ATTTTAATCA GTGCTCTTTG 
TTAGGAAAAT TACGACTGGA ATGTGCTGAC CTTCTAGAAA CAAGAGGAGT GGTGCTCCGT 
GACCCCACTC TGTTCTCTTT CCTTTGGGTG GTAGATTTCC CACTCTTCCT GCCCAAGGAG 
GAAAATCCCA GAGAGCTGGA ATCGGCCCAC CACCCATTTA CTGCTCCCCA CCCCAGTGAC 
ATACATCTCC TGTACACTGA GCCCAAAAAG GCCCGTAGCC AACACTATGA CTTGGTTTTA 
AATGGCAATG AAATAGGAGG TGGTTCAATT CGAATTCACA ATGCAGAGCT GCAGCGTTAT 
ATCCTGGCAA CCTTACTAAA GGAGGATGTG AAAATGCTCT CCCATCTGCT CCAGGCTTTA 
GATTATGGGG CACCCCCTCA TGGAGGAATT GCCTTAGGGT TAGACAGACT GATATGCCTT 
GTCACTGGAT CTCCAAGCAT CAGAGATGTC ATAGCCTTCC CAAAGTCCTT CCGGGGACAT 
GACCTCATGA GCAATACCCC AGATTCTGTC CCTCCTGAGG AACTGAAGCC CTATCATATC 
CGAGTCTCCA AGCCAACAGA CTCCAAAGCA GAAAGAGCTC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352141.1
CDS	558..2342
Translation	MYFPSWLSQLYRGLSRPIRRTTQPIWGSLYRSLLQSSQRRIPEFSSFVVRTNTCGELRSSHLGQEVTLCGWIQYRRQNTFLVLRDFDGLVQVIIPQDESAASVKKILCEAPVESVVQVSGTVISRPAGQENPKMPTGEIEIKVKTAELLNACKKLPFEIKNFVKKTEALRLQYRYLDLRSFQMQYNLRLRSQMVMKMREYLCNLHGFVDIETPTLFKRTPGGAKEFLVPSREPGKFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQPEFTQIDIEMSFVDQTGIQSLIEGLLQYSWPNDKDPVVVPFPTMTFAEVLATYGTDKPDTRFGMKIIDISDVFRNTEIGFLQDALSKPHGTVKAICIPEGAKYLKRKDIESIRNFAADHFNQCSLLGKLRLECADLLETRGVVLRDPTLFSFLWVVDFPLFLPKEENPRELESAHHPFTAPHPSDIHLLYTEPKKARSQHYDLVLNGNEIGGGSIRIHNAELQRYILATLLKEDVKMLSHLLQALDYGAPPHGGIALGLDRLICLVTGSPSIRDVIAFPKSFRGHDLMSNTPDSVPPEELKPYHIRVSKPTDSKAERAH

Target ORF information:

RefSeq Version	NM_001365212.1
Organism	Homo sapiens(human)
Definition	Homo sapiens aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365212.1

ORF Insert Sequence:

ATGTACTTCC CTTCTTGGTT AAGTCAGCTG TACAGGGGTT TATCCAGACC CATCAGAAGG 
ACCACCCAAC CGATCTGGGG TTCTCTCTAC AGAAGTCTGT TGCAGAGTTC ACAGAGGAGA 
ATTCCAGAAT TCAGTAGCTT TGTTGTCCGG ACCAACACAT GTGGAGAGTT GCGTTCGTCT 
CACTTAGGCC AAGAAGTCAC CTTGTGTGGA TGGATTCAGT ACCGAAGGCA AAACACATTC 
TTGGTCCTAA GAGATTTCGA TGGGCTTGTT CAAGTTATCA TTCCCCAGGA TGAGTCGGCA 
GCCTCTGTGA AGAAGATTTT ATGTGAAGCC CCTGTGGAAT CTGTGGTGCA AGTGTCTGGT 
ACAGTCATTT CCCGTCCTGC AGGACAAGAG AATCCAAAAA TGCCAACAGG TGAGATTGAA 
ATCAAAGTTA AAACAGCTGA GCTTCTGAAT GCCTGCAAGA AGCTGCCCTT TGAAATTAAG 
AACTTCGTGA AGAAAACAGA GGCTCTTCGG TTGCAGTATC GCTACTTAGA CTTGCGTAGT 
TTCCAAATGC AGTATAACCT GCGACTGAGG TCCCAGATGG TCATGAAAAT GCGGGAATAT 
CTCTGTAATC TGCATGGGTT TGTGGATATA GAAACCCCCA CATTGTTTAA GAGGACCCCA 
GGGGGTGCCA AAGAGTTTTT AGTACCATCC AGGGAACCTG GAAAGTTTTA TTCTCTCCCT 
CAGAGTCCTC AACAGTTTAA GCAACTTCTG ATGGTTGGCG GTTTAGACAG ATATTTTCAG 
GTTGCCCGAT GTTATCGAGA TGAAGGTTCA AGACCAGACA GACAGCCTGA GTTTACTCAG 
ATTGACATAG AGATGTCATT TGTAGACCAG ACTGGGATCC AGAGTTTAAT TGAGGGTTTG 
CTCCAGTATT CCTGGCCCAA TGACAAAGAT CCTGTGGTTG TTCCTTTTCC TACTATGACT 
TTTGCTGAGG TGCTGGCCAC CTATGGAACT GATAAACCTG ACACTCGCTT TGGAATGAAG 
ATTATAGATA TCAGTGATGT GTTTAGAAAC ACAGAGATTG GATTTCTTCA AGATGCACTT 
AGTAAGCCCC ATGGAACTGT GAAAGCCATA TGTATCCCTG AAGGAGCAAA ATACTTAAAA 
AGGAAAGACA TTGAATCCAT TAGAAACTTT GCAGCTGACC ATTTTAATCA GTGCTCTTTG 
TTAGGAAAAT TACGACTGGA ATGTGCTGAC CTTCTAGAAA CAAGAGGAGT GGTGCTCCGT 
GACCCCACTC TGTTCTCTTT CCTTTGGGTG GTAGATTTCC CACTCTTCCT GCCCAAGGAG 
GAAAATCCCA GAGAGCTGGA ATCGGCCCAC CACCCATTTA CTGCTCCCCA CCCCAGTGAC 
ATACATCTCC TGTACACTGA GCCCAAAAAG GCCCGTAGCC AACACTATGA CTTGGTTTTA 
AATGGCAATG AAATAGGAGG TGGTTCAATT CGAATTCACA ATGCAGAGCT GCAGCGTTAT 
ATCCTGGCAA CCTTACTAAA GGAGGATGTG AAAATGCTCT CCCATCTGCT CCAGGCTTTA 
GATTATGGGG CACCCCCTCA TGGAGGAATT GCCTTAGGGT TAGACAGACT GATATGCCTT 
GTCACTGGAT CTCCAAGCAT CAGAGATGTC ATAGCCTTCC CAAAGTCCTT CCGGGGACAT 
GACCTCATGA GCAATACCCC AGATTCTGTC CCTCCTGAGG AACTGAAGCC CTATCATATC 
CGAGTCTCCA AGCCAACAGA CTCCAAAGCA GAAAGAGCTC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DARS2 ( NM_001365213.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001352142.1 Homo sapiens aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu69095
Clone ID Related Accession (Same CDS sequence)	NM_001365213.2 , XM_011509711.2 , NM_001365213.1
Accession Version	NM_001365213.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	aspartate--tRNA ligase, mitochondrial isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL109921.21. On Jun 2, 2019 this sequence version replaced NM_001365213.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189664.174851.1, SRR1660803.46017.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGTACTTCC CTTCTTGGTT AAGTCAGCTG TACAGGGGTT TATCCAGACC CATCAGAAGG 
ACCACCCAAC CGATCTGGGG TTCTCTCTAC AGAAGTCTGT TGCAGAGTTC ACAGAGGAGA 
ATTCCAGAAT TCAGTAGCTT TGTTGTCCGG ACCAACACAT GTGGAGAGTT GCGTTCGTCT 
CACTTAGGCC AAGAAGTCAC CTTGTGTGGA TGGATTCAGT ACCGAAGGCA AAACACATTC 
TTGGTCCTAA GAGATTTCGA TGGGCTTGTT CAAGTTATCA TTCCCCAGGA TGAGTCGGCA 
GCCTCTGTGA AGAAGATTTT ATGTGAAGCC CCTGTGGAAT CTGTGGTGCA AGTGTCTGGT 
ACAGTCATTT CCCGTCCTGC AGGACAAGAG AATCCAAAAA TGCCAACAGG TGAGATTGAA 
ATCAAAGTTA AAACAGCTGA GCTTCTGAAT GCCTGCAAGA AGCTGCCCTT TGAAATTAAG 
AACTTCGTGA AGAAAACAGA GGCTCTTCGG TTGCAGTATC GCTACTTAGA CTTGCGTAGT 
TTCCAAATGC AGTATAACCT GCGACTGAGG TCCCAGATGG TCATGAAAAT GCGGGAATAT 
CTCTGTAATC TGCATGGGTT TGTGGATATA GAAACCCCCA CATTGTTTAA GAGGACCCCA 
GGGGGTGCCA AAGAGTTTTT AGTACCATCC AGGGAACCTG GAAAGTTTTA TTCTCTCCCT 
CAGAGTCCTC AACAGTTTAA GCAACTTCTG ATGGTTGGCG GTTTAGACAG ATATTTTCAG 
GTTGCCCGAT GTTATCGAGA TGAAGGTTCA AGACCAGACA GACAGCCTGA GTTTACTCAG 
ATTGACATAG AGATGTCATT TGTAGACCAG ACTGGGATCC AGAGTTTAAT TGAGGGTTTG 
CTCCAGTATT CCTGGCCCAA TGACAAAGAT CCTGTGGTTG TTCCTTTTCC TACTATGACT 
TTTGCTGAGG TGCTGGCCAC CTATGGAACT GATAAACCTG ACACTCGCTT TGGAATGAAG 
ATTATAGATA TCAGTGATGT GTTTAGAAAC ACAGAGATTG GATTTCTTCA AGATGCACTT 
AGTAAGCCCC ATGGAACTGT GAAAGCCATA TGTATCCCTG AAGGAGCAAA ATACTTAAAA 
AGGAAAGACA TTGAATCCAT TAGAAACTTT GCAGCTGACC ATTTTAATCA GGAAATCTTA 
CCTGTATTCC TTAACGCCAA TAGAAACTGG AATTCTCCAG TTGCTAATTT CATAATGGAG 
TCACAAAGAC TGGAATTAAT CAGACTAATG GAGACCCAAG AGGAAGATGT GGTCCTACTA 
ACTGCTGGAG AGCACAATAA AGCAAAAAAG TTTCAGAGTT CAGCTTCAAA GCTAAATGTA 
ATCCCCCGTC TCCCCTTCCC CTACAATAGT AAAATGAAGA AAGCTATTTA TTTCCAAATT 
TTATTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352142.1
CDS	558..2006
Translation	MYFPSWLSQLYRGLSRPIRRTTQPIWGSLYRSLLQSSQRRIPEFSSFVVRTNTCGELRSSHLGQEVTLCGWIQYRRQNTFLVLRDFDGLVQVIIPQDESAASVKKILCEAPVESVVQVSGTVISRPAGQENPKMPTGEIEIKVKTAELLNACKKLPFEIKNFVKKTEALRLQYRYLDLRSFQMQYNLRLRSQMVMKMREYLCNLHGFVDIETPTLFKRTPGGAKEFLVPSREPGKFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQPEFTQIDIEMSFVDQTGIQSLIEGLLQYSWPNDKDPVVVPFPTMTFAEVLATYGTDKPDTRFGMKIIDISDVFRNTEIGFLQDALSKPHGTVKAICIPEGAKYLKRKDIESIRNFAADHFNQEILPVFLNANRNWNSPVANFIMESQRLELIRLMETQEEDVVLLTAGEHNKAKKFQSSASKLNVIPRLPFPYNSKMKKAIYFQILL

Target ORF information:

RefSeq Version	NM_001365213.2
Organism	Homo sapiens(human)
Definition	Homo sapiens aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365213.2

ORF Insert Sequence:

ATGTACTTCC CTTCTTGGTT AAGTCAGCTG TACAGGGGTT TATCCAGACC CATCAGAAGG 
ACCACCCAAC CGATCTGGGG TTCTCTCTAC AGAAGTCTGT TGCAGAGTTC ACAGAGGAGA 
ATTCCAGAAT TCAGTAGCTT TGTTGTCCGG ACCAACACAT GTGGAGAGTT GCGTTCGTCT 
CACTTAGGCC AAGAAGTCAC CTTGTGTGGA TGGATTCAGT ACCGAAGGCA AAACACATTC 
TTGGTCCTAA GAGATTTCGA TGGGCTTGTT CAAGTTATCA TTCCCCAGGA TGAGTCGGCA 
GCCTCTGTGA AGAAGATTTT ATGTGAAGCC CCTGTGGAAT CTGTGGTGCA AGTGTCTGGT 
ACAGTCATTT CCCGTCCTGC AGGACAAGAG AATCCAAAAA TGCCAACAGG TGAGATTGAA 
ATCAAAGTTA AAACAGCTGA GCTTCTGAAT GCCTGCAAGA AGCTGCCCTT TGAAATTAAG 
AACTTCGTGA AGAAAACAGA GGCTCTTCGG TTGCAGTATC GCTACTTAGA CTTGCGTAGT 
TTCCAAATGC AGTATAACCT GCGACTGAGG TCCCAGATGG TCATGAAAAT GCGGGAATAT 
CTCTGTAATC TGCATGGGTT TGTGGATATA GAAACCCCCA CATTGTTTAA GAGGACCCCA 
GGGGGTGCCA AAGAGTTTTT AGTACCATCC AGGGAACCTG GAAAGTTTTA TTCTCTCCCT 
CAGAGTCCTC AACAGTTTAA GCAACTTCTG ATGGTTGGCG GTTTAGACAG ATATTTTCAG 
GTTGCCCGAT GTTATCGAGA TGAAGGTTCA AGACCAGACA GACAGCCTGA GTTTACTCAG 
ATTGACATAG AGATGTCATT TGTAGACCAG ACTGGGATCC AGAGTTTAAT TGAGGGTTTG 
CTCCAGTATT CCTGGCCCAA TGACAAAGAT CCTGTGGTTG TTCCTTTTCC TACTATGACT 
TTTGCTGAGG TGCTGGCCAC CTATGGAACT GATAAACCTG ACACTCGCTT TGGAATGAAG 
ATTATAGATA TCAGTGATGT GTTTAGAAAC ACAGAGATTG GATTTCTTCA AGATGCACTT 
AGTAAGCCCC ATGGAACTGT GAAAGCCATA TGTATCCCTG AAGGAGCAAA ATACTTAAAA 
AGGAAAGACA TTGAATCCAT TAGAAACTTT GCAGCTGACC ATTTTAATCA GGAAATCTTA 
CCTGTATTCC TTAACGCCAA TAGAAACTGG AATTCTCCAG TTGCTAATTT CATAATGGAG 
TCACAAAGAC TGGAATTAAT CAGACTAATG GAGACCCAAG AGGAAGATGT GGTCCTACTA 
ACTGCTGGAG AGCACAATAA AGCAAAAAAG TTTCAGAGTT CAGCTTCAAA GCTAAATGTA 
ATCCCCCGTC TCCCCTTCCC CTACAATAGT AAAATGAAGA AAGCTATTTA TTTCCAAATT 
TTATTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DARS2 ( NM_001365213.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001352142.1 Homo sapiens aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu69095
Clone ID Related Accession (Same CDS sequence)	NM_001365213.2 , XM_011509711.2 , NM_001365213.1
Accession Version	NM_001365213.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	aspartate--tRNA ligase, mitochondrial isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL109921.21. On Aug 7, 2018 this sequence version replaced XM_011509711.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189664.174851.1, SRR1803615.234215.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGTACTTCC CTTCTTGGTT AAGTCAGCTG TACAGGGGTT TATCCAGACC CATCAGAAGG 
ACCACCCAAC CGATCTGGGG TTCTCTCTAC AGAAGTCTGT TGCAGAGTTC ACAGAGGAGA 
ATTCCAGAAT TCAGTAGCTT TGTTGTCCGG ACCAACACAT GTGGAGAGTT GCGTTCGTCT 
CACTTAGGCC AAGAAGTCAC CTTGTGTGGA TGGATTCAGT ACCGAAGGCA AAACACATTC 
TTGGTCCTAA GAGATTTCGA TGGGCTTGTT CAAGTTATCA TTCCCCAGGA TGAGTCGGCA 
GCCTCTGTGA AGAAGATTTT ATGTGAAGCC CCTGTGGAAT CTGTGGTGCA AGTGTCTGGT 
ACAGTCATTT CCCGTCCTGC AGGACAAGAG AATCCAAAAA TGCCAACAGG TGAGATTGAA 
ATCAAAGTTA AAACAGCTGA GCTTCTGAAT GCCTGCAAGA AGCTGCCCTT TGAAATTAAG 
AACTTCGTGA AGAAAACAGA GGCTCTTCGG TTGCAGTATC GCTACTTAGA CTTGCGTAGT 
TTCCAAATGC AGTATAACCT GCGACTGAGG TCCCAGATGG TCATGAAAAT GCGGGAATAT 
CTCTGTAATC TGCATGGGTT TGTGGATATA GAAACCCCCA CATTGTTTAA GAGGACCCCA 
GGGGGTGCCA AAGAGTTTTT AGTACCATCC AGGGAACCTG GAAAGTTTTA TTCTCTCCCT 
CAGAGTCCTC AACAGTTTAA GCAACTTCTG ATGGTTGGCG GTTTAGACAG ATATTTTCAG 
GTTGCCCGAT GTTATCGAGA TGAAGGTTCA AGACCAGACA GACAGCCTGA GTTTACTCAG 
ATTGACATAG AGATGTCATT TGTAGACCAG ACTGGGATCC AGAGTTTAAT TGAGGGTTTG 
CTCCAGTATT CCTGGCCCAA TGACAAAGAT CCTGTGGTTG TTCCTTTTCC TACTATGACT 
TTTGCTGAGG TGCTGGCCAC CTATGGAACT GATAAACCTG ACACTCGCTT TGGAATGAAG 
ATTATAGATA TCAGTGATGT GTTTAGAAAC ACAGAGATTG GATTTCTTCA AGATGCACTT 
AGTAAGCCCC ATGGAACTGT GAAAGCCATA TGTATCCCTG AAGGAGCAAA ATACTTAAAA 
AGGAAAGACA TTGAATCCAT TAGAAACTTT GCAGCTGACC ATTTTAATCA GGAAATCTTA 
CCTGTATTCC TTAACGCCAA TAGAAACTGG AATTCTCCAG TTGCTAATTT CATAATGGAG 
TCACAAAGAC TGGAATTAAT CAGACTAATG GAGACCCAAG AGGAAGATGT GGTCCTACTA 
ACTGCTGGAG AGCACAATAA AGCAAAAAAG TTTCAGAGTT CAGCTTCAAA GCTAAATGTA 
ATCCCCCGTC TCCCCTTCCC CTACAATAGT AAAATGAAGA AAGCTATTTA TTTCCAAATT 
TTATTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352142.1
CDS	558..2006
Translation	MYFPSWLSQLYRGLSRPIRRTTQPIWGSLYRSLLQSSQRRIPEFSSFVVRTNTCGELRSSHLGQEVTLCGWIQYRRQNTFLVLRDFDGLVQVIIPQDESAASVKKILCEAPVESVVQVSGTVISRPAGQENPKMPTGEIEIKVKTAELLNACKKLPFEIKNFVKKTEALRLQYRYLDLRSFQMQYNLRLRSQMVMKMREYLCNLHGFVDIETPTLFKRTPGGAKEFLVPSREPGKFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQPEFTQIDIEMSFVDQTGIQSLIEGLLQYSWPNDKDPVVVPFPTMTFAEVLATYGTDKPDTRFGMKIIDISDVFRNTEIGFLQDALSKPHGTVKAICIPEGAKYLKRKDIESIRNFAADHFNQEILPVFLNANRNWNSPVANFIMESQRLELIRLMETQEEDVVLLTAGEHNKAKKFQSSASKLNVIPRLPFPYNSKMKKAIYFQILL

Target ORF information:

RefSeq Version	NM_001365213.1
Organism	Homo sapiens(human)
Definition	Homo sapiens aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365213.1

ORF Insert Sequence:

ATGTACTTCC CTTCTTGGTT AAGTCAGCTG TACAGGGGTT TATCCAGACC CATCAGAAGG 
ACCACCCAAC CGATCTGGGG TTCTCTCTAC AGAAGTCTGT TGCAGAGTTC ACAGAGGAGA 
ATTCCAGAAT TCAGTAGCTT TGTTGTCCGG ACCAACACAT GTGGAGAGTT GCGTTCGTCT 
CACTTAGGCC AAGAAGTCAC CTTGTGTGGA TGGATTCAGT ACCGAAGGCA AAACACATTC 
TTGGTCCTAA GAGATTTCGA TGGGCTTGTT CAAGTTATCA TTCCCCAGGA TGAGTCGGCA 
GCCTCTGTGA AGAAGATTTT ATGTGAAGCC CCTGTGGAAT CTGTGGTGCA AGTGTCTGGT 
ACAGTCATTT CCCGTCCTGC AGGACAAGAG AATCCAAAAA TGCCAACAGG TGAGATTGAA 
ATCAAAGTTA AAACAGCTGA GCTTCTGAAT GCCTGCAAGA AGCTGCCCTT TGAAATTAAG 
AACTTCGTGA AGAAAACAGA GGCTCTTCGG TTGCAGTATC GCTACTTAGA CTTGCGTAGT 
TTCCAAATGC AGTATAACCT GCGACTGAGG TCCCAGATGG TCATGAAAAT GCGGGAATAT 
CTCTGTAATC TGCATGGGTT TGTGGATATA GAAACCCCCA CATTGTTTAA GAGGACCCCA 
GGGGGTGCCA AAGAGTTTTT AGTACCATCC AGGGAACCTG GAAAGTTTTA TTCTCTCCCT 
CAGAGTCCTC AACAGTTTAA GCAACTTCTG ATGGTTGGCG GTTTAGACAG ATATTTTCAG 
GTTGCCCGAT GTTATCGAGA TGAAGGTTCA AGACCAGACA GACAGCCTGA GTTTACTCAG 
ATTGACATAG AGATGTCATT TGTAGACCAG ACTGGGATCC AGAGTTTAAT TGAGGGTTTG 
CTCCAGTATT CCTGGCCCAA TGACAAAGAT CCTGTGGTTG TTCCTTTTCC TACTATGACT 
TTTGCTGAGG TGCTGGCCAC CTATGGAACT GATAAACCTG ACACTCGCTT TGGAATGAAG 
ATTATAGATA TCAGTGATGT GTTTAGAAAC ACAGAGATTG GATTTCTTCA AGATGCACTT 
AGTAAGCCCC ATGGAACTGT GAAAGCCATA TGTATCCCTG AAGGAGCAAA ATACTTAAAA 
AGGAAAGACA TTGAATCCAT TAGAAACTTT GCAGCTGACC ATTTTAATCA GGAAATCTTA 
CCTGTATTCC TTAACGCCAA TAGAAACTGG AATTCTCCAG TTGCTAATTT CATAATGGAG 
TCACAAAGAC TGGAATTAAT CAGACTAATG GAGACCCAAG AGGAAGATGT GGTCCTACTA 
ACTGCTGGAG AGCACAATAA AGCAAAAAAG TTTCAGAGTT CAGCTTCAAA GCTAAATGTA 
ATCCCCCGTC TCCCCTTCCC CTACAATAGT AAAATGAAGA AAGCTATTTA TTTCCAAATT 
TTATTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DARS2 ( XM_006711427.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006711490.1 Homo sapiens aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant X1, mRNA.

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CloneID	OHu34519
Clone ID Related Accession (Same CDS sequence)	XM_006711427.3 , NM_001365212.1
Accession Version	XM_006711427.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	aspartate--tRNA ligase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006711427.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTCC CTTCTTGGTT AAGTCAGCTG TACAGGGGTT TATCCAGACC CATCAGAAGG 
ACCACCCAAC CGATCTGGGG TTCTCTCTAC AGAAGTCTGT TGCAGAGTTC ACAGAGGAGA 
ATTCCAGAAT TCAGTAGCTT TGTTGTCCGG ACCAACACAT GTGGAGAGTT GCGTTCGTCT 
CACTTAGGCC AAGAAGTCAC CTTGTGTGGA TGGATTCAGT ACCGAAGGCA AAACACATTC 
TTGGTCCTAA GAGATTTCGA TGGGCTTGTT CAAGTTATCA TTCCCCAGGA TGAGTCGGCA 
GCCTCTGTGA AGAAGATTTT ATGTGAAGCC CCTGTGGAAT CTGTGGTGCA AGTGTCTGGT 
ACAGTCATTT CCCGTCCTGC AGGACAAGAG AATCCAAAAA TGCCAACAGG TGAGATTGAA 
ATCAAAGTTA AAACAGCTGA GCTTCTGAAT GCCTGCAAGA AGCTGCCCTT TGAAATTAAG 
AACTTCGTGA AGAAAACAGA GGCTCTTCGG TTGCAGTATC GCTACTTAGA CTTGCGTAGT 
TTCCAAATGC AGTATAACCT GCGACTGAGG TCCCAGATGG TCATGAAAAT GCGGGAATAT 
CTCTGTAATC TGCATGGGTT TGTGGATATA GAAACCCCCA CATTGTTTAA GAGGACCCCA 
GGGGGTGCCA AAGAGTTTTT AGTACCATCC AGGGAACCTG GAAAGTTTTA TTCTCTCCCT 
CAGAGTCCTC AACAGTTTAA GCAACTTCTG ATGGTTGGCG GTTTAGACAG ATATTTTCAG 
GTTGCCCGAT GTTATCGAGA TGAAGGTTCA AGACCAGACA GACAGCCTGA GTTTACTCAG 
ATTGACATAG AGATGTCATT TGTAGACCAG ACTGGGATCC AGAGTTTAAT TGAGGGTTTG 
CTCCAGTATT CCTGGCCCAA TGACAAAGAT CCTGTGGTTG TTCCTTTTCC TACTATGACT 
TTTGCTGAGG TGCTGGCCAC CTATGGAACT GATAAACCTG ACACTCGCTT TGGAATGAAG 
ATTATAGATA TCAGTGATGT GTTTAGAAAC ACAGAGATTG GATTTCTTCA AGATGCACTT 
AGTAAGCCCC ATGGAACTGT GAAAGCCATA TGTATCCCTG AAGGAGCAAA ATACTTAAAA 
AGGAAAGACA TTGAATCCAT TAGAAACTTT GCAGCTGACC ATTTTAATCA GTGCTCTTTG 
TTAGGAAAAT TACGACTGGA ATGTGCTGAC CTTCTAGAAA CAAGAGGAGT GGTGCTCCGT 
GACCCCACTC TGTTCTCTTT CCTTTGGGTG GTAGATTTCC CACTCTTCCT GCCCAAGGAG 
GAAAATCCCA GAGAGCTGGA ATCGGCCCAC CACCCATTTA CTGCTCCCCA CCCCAGTGAC 
ATACATCTCC TGTACACTGA GCCCAAAAAG GCCCGTAGCC AACACTATGA CTTGGTTTTA 
AATGGCAATG AAATAGGAGG TGGTTCAATT CGAATTCACA ATGCAGAGCT GCAGCGTTAT 
ATCCTGGCAA CCTTACTAAA GGAGGATGTG AAAATGCTCT CCCATCTGCT CCAGGCTTTA 
GATTATGGGG CACCCCCTCA TGGAGGAATT GCCTTAGGGT TAGACAGACT GATATGCCTT 
GTCACTGGAT CTCCAAGCAT CAGAGATGTC ATAGCCTTCC CAAAGTCCTT CCGGGGACAT 
GACCTCATGA GCAATACCCC AGATTCTGTC CCTCCTGAGG AACTGAAGCC CTATCATATC 
CGAGTCTCCA AGCCAACAGA CTCCAAAGCA GAAAGAGCTC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006711490.1
CDS	529..2313
Translation	MYFPSWLSQLYRGLSRPIRRTTQPIWGSLYRSLLQSSQRRIPEFSSFVVRTNTCGELRSSHLGQEVTLCGWIQYRRQNTFLVLRDFDGLVQVIIPQDESAASVKKILCEAPVESVVQVSGTVISRPAGQENPKMPTGEIEIKVKTAELLNACKKLPFEIKNFVKKTEALRLQYRYLDLRSFQMQYNLRLRSQMVMKMREYLCNLHGFVDIETPTLFKRTPGGAKEFLVPSREPGKFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQPEFTQIDIEMSFVDQTGIQSLIEGLLQYSWPNDKDPVVVPFPTMTFAEVLATYGTDKPDTRFGMKIIDISDVFRNTEIGFLQDALSKPHGTVKAICIPEGAKYLKRKDIESIRNFAADHFNQCSLLGKLRLECADLLETRGVVLRDPTLFSFLWVVDFPLFLPKEENPRELESAHHPFTAPHPSDIHLLYTEPKKARSQHYDLVLNGNEIGGGSIRIHNAELQRYILATLLKEDVKMLSHLLQALDYGAPPHGGIALGLDRLICLVTGSPSIRDVIAFPKSFRGHDLMSNTPDSVPPEELKPYHIRVSKPTDSKAERAH

Target ORF information:

RefSeq Version	XM_006711427.3
Organism	Homo sapiens(human)
Definition	Homo sapiens aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006711427.3

ORF Insert Sequence:

ATGTACTTCC CTTCTTGGTT AAGTCAGCTG TACAGGGGTT TATCCAGACC CATCAGAAGG 
ACCACCCAAC CGATCTGGGG TTCTCTCTAC AGAAGTCTGT TGCAGAGTTC ACAGAGGAGA 
ATTCCAGAAT TCAGTAGCTT TGTTGTCCGG ACCAACACAT GTGGAGAGTT GCGTTCGTCT 
CACTTAGGCC AAGAAGTCAC CTTGTGTGGA TGGATTCAGT ACCGAAGGCA AAACACATTC 
TTGGTCCTAA GAGATTTCGA TGGGCTTGTT CAAGTTATCA TTCCCCAGGA TGAGTCGGCA 
GCCTCTGTGA AGAAGATTTT ATGTGAAGCC CCTGTGGAAT CTGTGGTGCA AGTGTCTGGT 
ACAGTCATTT CCCGTCCTGC AGGACAAGAG AATCCAAAAA TGCCAACAGG TGAGATTGAA 
ATCAAAGTTA AAACAGCTGA GCTTCTGAAT GCCTGCAAGA AGCTGCCCTT TGAAATTAAG 
AACTTCGTGA AGAAAACAGA GGCTCTTCGG TTGCAGTATC GCTACTTAGA CTTGCGTAGT 
TTCCAAATGC AGTATAACCT GCGACTGAGG TCCCAGATGG TCATGAAAAT GCGGGAATAT 
CTCTGTAATC TGCATGGGTT TGTGGATATA GAAACCCCCA CATTGTTTAA GAGGACCCCA 
GGGGGTGCCA AAGAGTTTTT AGTACCATCC AGGGAACCTG GAAAGTTTTA TTCTCTCCCT 
CAGAGTCCTC AACAGTTTAA GCAACTTCTG ATGGTTGGCG GTTTAGACAG ATATTTTCAG 
GTTGCCCGAT GTTATCGAGA TGAAGGTTCA AGACCAGACA GACAGCCTGA GTTTACTCAG 
ATTGACATAG AGATGTCATT TGTAGACCAG ACTGGGATCC AGAGTTTAAT TGAGGGTTTG 
CTCCAGTATT CCTGGCCCAA TGACAAAGAT CCTGTGGTTG TTCCTTTTCC TACTATGACT 
TTTGCTGAGG TGCTGGCCAC CTATGGAACT GATAAACCTG ACACTCGCTT TGGAATGAAG 
ATTATAGATA TCAGTGATGT GTTTAGAAAC ACAGAGATTG GATTTCTTCA AGATGCACTT 
AGTAAGCCCC ATGGAACTGT GAAAGCCATA TGTATCCCTG AAGGAGCAAA ATACTTAAAA 
AGGAAAGACA TTGAATCCAT TAGAAACTTT GCAGCTGACC ATTTTAATCA GTGCTCTTTG 
TTAGGAAAAT TACGACTGGA ATGTGCTGAC CTTCTAGAAA CAAGAGGAGT GGTGCTCCGT 
GACCCCACTC TGTTCTCTTT CCTTTGGGTG GTAGATTTCC CACTCTTCCT GCCCAAGGAG 
GAAAATCCCA GAGAGCTGGA ATCGGCCCAC CACCCATTTA CTGCTCCCCA CCCCAGTGAC 
ATACATCTCC TGTACACTGA GCCCAAAAAG GCCCGTAGCC AACACTATGA CTTGGTTTTA 
AATGGCAATG AAATAGGAGG TGGTTCAATT CGAATTCACA ATGCAGAGCT GCAGCGTTAT 
ATCCTGGCAA CCTTACTAAA GGAGGATGTG AAAATGCTCT CCCATCTGCT CCAGGCTTTA 
GATTATGGGG CACCCCCTCA TGGAGGAATT GCCTTAGGGT TAGACAGACT GATATGCCTT 
GTCACTGGAT CTCCAAGCAT CAGAGATGTC ATAGCCTTCC CAAAGTCCTT CCGGGGACAT 
GACCTCATGA GCAATACCCC AGATTCTGTC CCTCCTGAGG AACTGAAGCC CTATCATATC 
CGAGTCTCCA AGCCAACAGA CTCCAAAGCA GAAAGAGCTC ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DARS2 ( XM_011509711.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508013.1 Homo sapiens aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant X2, mRNA.

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CloneID	OHu69095
Clone ID Related Accession (Same CDS sequence)	NM_001365213.2 , XM_011509711.2 , NM_001365213.1
Accession Version	XM_011509711.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	aspartate--tRNA ligase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011509711.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTCC CTTCTTGGTT AAGTCAGCTG TACAGGGGTT TATCCAGACC CATCAGAAGG 
ACCACCCAAC CGATCTGGGG TTCTCTCTAC AGAAGTCTGT TGCAGAGTTC ACAGAGGAGA 
ATTCCAGAAT TCAGTAGCTT TGTTGTCCGG ACCAACACAT GTGGAGAGTT GCGTTCGTCT 
CACTTAGGCC AAGAAGTCAC CTTGTGTGGA TGGATTCAGT ACCGAAGGCA AAACACATTC 
TTGGTCCTAA GAGATTTCGA TGGGCTTGTT CAAGTTATCA TTCCCCAGGA TGAGTCGGCA 
GCCTCTGTGA AGAAGATTTT ATGTGAAGCC CCTGTGGAAT CTGTGGTGCA AGTGTCTGGT 
ACAGTCATTT CCCGTCCTGC AGGACAAGAG AATCCAAAAA TGCCAACAGG TGAGATTGAA 
ATCAAAGTTA AAACAGCTGA GCTTCTGAAT GCCTGCAAGA AGCTGCCCTT TGAAATTAAG 
AACTTCGTGA AGAAAACAGA GGCTCTTCGG TTGCAGTATC GCTACTTAGA CTTGCGTAGT 
TTCCAAATGC AGTATAACCT GCGACTGAGG TCCCAGATGG TCATGAAAAT GCGGGAATAT 
CTCTGTAATC TGCATGGGTT TGTGGATATA GAAACCCCCA CATTGTTTAA GAGGACCCCA 
GGGGGTGCCA AAGAGTTTTT AGTACCATCC AGGGAACCTG GAAAGTTTTA TTCTCTCCCT 
CAGAGTCCTC AACAGTTTAA GCAACTTCTG ATGGTTGGCG GTTTAGACAG ATATTTTCAG 
GTTGCCCGAT GTTATCGAGA TGAAGGTTCA AGACCAGACA GACAGCCTGA GTTTACTCAG 
ATTGACATAG AGATGTCATT TGTAGACCAG ACTGGGATCC AGAGTTTAAT TGAGGGTTTG 
CTCCAGTATT CCTGGCCCAA TGACAAAGAT CCTGTGGTTG TTCCTTTTCC TACTATGACT 
TTTGCTGAGG TGCTGGCCAC CTATGGAACT GATAAACCTG ACACTCGCTT TGGAATGAAG 
ATTATAGATA TCAGTGATGT GTTTAGAAAC ACAGAGATTG GATTTCTTCA AGATGCACTT 
AGTAAGCCCC ATGGAACTGT GAAAGCCATA TGTATCCCTG AAGGAGCAAA ATACTTAAAA 
AGGAAAGACA TTGAATCCAT TAGAAACTTT GCAGCTGACC ATTTTAATCA GGAAATCTTA 
CCTGTATTCC TTAACGCCAA TAGAAACTGG AATTCTCCAG TTGCTAATTT CATAATGGAG 
TCACAAAGAC TGGAATTAAT CAGACTAATG GAGACCCAAG AGGAAGATGT GGTCCTACTA 
ACTGCTGGAG AGCACAATAA AGCAAAAAAG TTTCAGAGTT CAGCTTCAAA GCTAAATGTA 
ATCCCCCGTC TCCCCTTCCC CTACAATAGT AAAATGAAGA AAGCTATTTA TTTCCAAATT 
TTATTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508013.1
CDS	529..1977
Translation	MYFPSWLSQLYRGLSRPIRRTTQPIWGSLYRSLLQSSQRRIPEFSSFVVRTNTCGELRSSHLGQEVTLCGWIQYRRQNTFLVLRDFDGLVQVIIPQDESAASVKKILCEAPVESVVQVSGTVISRPAGQENPKMPTGEIEIKVKTAELLNACKKLPFEIKNFVKKTEALRLQYRYLDLRSFQMQYNLRLRSQMVMKMREYLCNLHGFVDIETPTLFKRTPGGAKEFLVPSREPGKFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQPEFTQIDIEMSFVDQTGIQSLIEGLLQYSWPNDKDPVVVPFPTMTFAEVLATYGTDKPDTRFGMKIIDISDVFRNTEIGFLQDALSKPHGTVKAICIPEGAKYLKRKDIESIRNFAADHFNQEILPVFLNANRNWNSPVANFIMESQRLELIRLMETQEEDVVLLTAGEHNKAKKFQSSASKLNVIPRLPFPYNSKMKKAIYFQILL

Target ORF information:

RefSeq Version	XM_011509711.2
Organism	Homo sapiens(human)
Definition	Homo sapiens aspartyl-tRNA synthetase 2, mitochondrial (DARS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509711.2

ORF Insert Sequence:

ATGTACTTCC CTTCTTGGTT AAGTCAGCTG TACAGGGGTT TATCCAGACC CATCAGAAGG 
ACCACCCAAC CGATCTGGGG TTCTCTCTAC AGAAGTCTGT TGCAGAGTTC ACAGAGGAGA 
ATTCCAGAAT TCAGTAGCTT TGTTGTCCGG ACCAACACAT GTGGAGAGTT GCGTTCGTCT 
CACTTAGGCC AAGAAGTCAC CTTGTGTGGA TGGATTCAGT ACCGAAGGCA AAACACATTC 
TTGGTCCTAA GAGATTTCGA TGGGCTTGTT CAAGTTATCA TTCCCCAGGA TGAGTCGGCA 
GCCTCTGTGA AGAAGATTTT ATGTGAAGCC CCTGTGGAAT CTGTGGTGCA AGTGTCTGGT 
ACAGTCATTT CCCGTCCTGC AGGACAAGAG AATCCAAAAA TGCCAACAGG TGAGATTGAA 
ATCAAAGTTA AAACAGCTGA GCTTCTGAAT GCCTGCAAGA AGCTGCCCTT TGAAATTAAG 
AACTTCGTGA AGAAAACAGA GGCTCTTCGG TTGCAGTATC GCTACTTAGA CTTGCGTAGT 
TTCCAAATGC AGTATAACCT GCGACTGAGG TCCCAGATGG TCATGAAAAT GCGGGAATAT 
CTCTGTAATC TGCATGGGTT TGTGGATATA GAAACCCCCA CATTGTTTAA GAGGACCCCA 
GGGGGTGCCA AAGAGTTTTT AGTACCATCC AGGGAACCTG GAAAGTTTTA TTCTCTCCCT 
CAGAGTCCTC AACAGTTTAA GCAACTTCTG ATGGTTGGCG GTTTAGACAG ATATTTTCAG 
GTTGCCCGAT GTTATCGAGA TGAAGGTTCA AGACCAGACA GACAGCCTGA GTTTACTCAG 
ATTGACATAG AGATGTCATT TGTAGACCAG ACTGGGATCC AGAGTTTAAT TGAGGGTTTG 
CTCCAGTATT CCTGGCCCAA TGACAAAGAT CCTGTGGTTG TTCCTTTTCC TACTATGACT 
TTTGCTGAGG TGCTGGCCAC CTATGGAACT GATAAACCTG ACACTCGCTT TGGAATGAAG 
ATTATAGATA TCAGTGATGT GTTTAGAAAC ACAGAGATTG GATTTCTTCA AGATGCACTT 
AGTAAGCCCC ATGGAACTGT GAAAGCCATA TGTATCCCTG AAGGAGCAAA ATACTTAAAA 
AGGAAAGACA TTGAATCCAT TAGAAACTTT GCAGCTGACC ATTTTAATCA GGAAATCTTA 
CCTGTATTCC TTAACGCCAA TAGAAACTGG AATTCTCCAG TTGCTAATTT CATAATGGAG 
TCACAAAGAC TGGAATTAAT CAGACTAATG GAGACCCAAG AGGAAGATGT GGTCCTACTA 
ACTGCTGGAG AGCACAATAA AGCAAAAAAG TTTCAGAGTT CAGCTTCAAA GCTAAATGTA 
ATCCCCCGTC TCCCCTTCCC CTACAATAGT AAAATGAAGA AAGCTATTTA TTTCCAAATT 
TTATTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Upregulation of DARS2 by HBV promotes hepatocarcinogenesis through the miR-30e-5p/MAPK/NFAT5 pathway.
Journal of experimental & clinical cancer research : CR36(1)148(2017 Oct)
Qin X,Li C,Guo T,Chen J,Wang HT,Wang YT,Xiao YS,Li J,Liu P,Liu ZS,Liu QY

Neurodegenerative disease-associated mutants of a human mitochondrial aminoacyl-tRNA synthetase present individual molecular signatures.
Scientific reports517332(2015 Dec)
Sauter C,Lorber B,Gaudry A,Karim L,Schwenzer H,Wien F,Roblin P,Florentz C,Sissler M

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DARS2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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