ORF » Species Summary » Homo sapiens » PPP2CA cDNA ORF clone

PPP2CA cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following PPP2CA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPP2CA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu20615 NM_002715.4
Latest version!		 Homo sapiens protein phosphatase 2 catalytic subunit alpha (PPP2CA), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$154.50-$216.30
$309.00
OHu102930 NM_001355019.1
Latest version!		 Homo sapiens protein phosphatase 2 catalytic subunit alpha (PPP2CA), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
View Old Accession Versions >>
OHu20615 NM_002715.3 Homo sapiens protein phosphatase 2 catalytic subunit alpha (PPP2CA), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$154.50-$216.30
$309.00
OHu20615 NM_002715.2 Homo sapiens protein phosphatase 2 catalytic subunit alpha (PPP2CA), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$154.50-$216.30
$309.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    PPP2CA ( NM_002715.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002706.1 Homo sapiens protein phosphatase 2 catalytic subunit alpha (PPP2CA), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu20615
    Clone ID Related Accession (Same CDS sequence) NM_002715.3 , NM_002715.4 , NM_002715.2
    Accession Version NM_002715.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 930bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-27
    Organism Homo sapiens(human)
    Product serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104109.3 and AK097599.1. This sequence is a reference standard in the RefSeqGene project. On Sep 1, 2017 this sequence version replaced NM_002715.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.9460.1, SRR1660805.119337.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820687, SAMN03267764 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGACGAGA AGGTGTTCAC CAAGGAGCTG GACCAGTGGA TCGAGCAGCT GAACGAGTGC 
    AAGCAGCTGT CCGAGTCCCA GGTCAAGAGC CTCTGCGAGA AGGCTAAAGA AATCCTGACA 
    AAAGAATCCA ACGTGCAAGA GGTTCGATGT CCAGTTACTG TCTGTGGAGA TGTGCATGGG 
    CAATTTCATG ATCTCATGGA ACTGTTTAGA ATTGGTGGCA AATCACCAGA TACAAATTAC 
    TTGTTTATGG GAGATTATGT TGACAGAGGA TATTATTCAG TTGAAACAGT TACACTGCTT 
    GTAGCTCTTA AGGTTCGTTA CCGTGAACGC ATCACCATTC TTCGAGGGAA TCATGAGAGC 
    AGACAGATCA CACAAGTTTA TGGTTTCTAT GATGAATGTT TAAGAAAATA TGGAAATGCA 
    AATGTTTGGA AATATTTTAC AGATCTTTTT GACTATCTTC CTCTCACTGC CTTGGTGGAT 
    GGGCAGATCT TCTGTCTACA TGGTGGTCTC TCGCCATCTA TAGATACACT GGATCATATC 
    AGAGCACTTG ATCGCCTACA AGAAGTTCCC CATGAGGGTC CAATGTGTGA CTTGCTGTGG 
    TCAGATCCAG ATGACCGTGG TGGTTGGGGT ATATCTCCTC GAGGAGCTGG TTACACCTTT 
    GGGCAAGATA TTTCTGAGAC ATTTAATCAT GCCAATGGCC TCACGTTGGT GTCTAGAGCT 
    CACCAGCTAG TGATGGAGGG ATATAACTGG TGCCATGACC GGAATGTAGT AACGATTTTC 
    AGTGCTCCAA ACTATTGTTA TCGTTGTGGT AACCAAGCTG CAATCATGGA ACTTGACGAT 
    ACTCTAAAAT ACTCTTTCTT GCAGTTTGAC CCAGCACCTC GTAGAGGCGA GCCACATGTT 
    ACTCGTCGTA CCCCAGACTA CTTCCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002706.1
    CDS399..1328
    Translation

    Target ORF information:

    RefSeq Version NM_002715.3
    Organism Homo sapiens(human)
    Definition Homo sapiens protein phosphatase 2 catalytic subunit alpha (PPP2CA), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_002715.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGACGAGA AGGTGTTCAC CAAGGAGCTG GACCAGTGGA TCGAGCAGCT GAACGAGTGC 
    AAGCAGCTGT CCGAGTCCCA GGTCAAGAGC CTCTGCGAGA AGGCTAAAGA AATCCTGACA 
    AAAGAATCCA ACGTGCAAGA GGTTCGATGT CCAGTTACTG TCTGTGGAGA TGTGCATGGG 
    CAATTTCATG ATCTCATGGA ACTGTTTAGA ATTGGTGGCA AATCACCAGA TACAAATTAC 
    TTGTTTATGG GAGATTATGT TGACAGAGGA TATTATTCAG TTGAAACAGT TACACTGCTT 
    GTAGCTCTTA AGGTTCGTTA CCGTGAACGC ATCACCATTC TTCGAGGGAA TCATGAGAGC 
    AGACAGATCA CACAAGTTTA TGGTTTCTAT GATGAATGTT TAAGAAAATA TGGAAATGCA 
    AATGTTTGGA AATATTTTAC AGATCTTTTT GACTATCTTC CTCTCACTGC CTTGGTGGAT 
    GGGCAGATCT TCTGTCTACA TGGTGGTCTC TCGCCATCTA TAGATACACT GGATCATATC 
    AGAGCACTTG ATCGCCTACA AGAAGTTCCC CATGAGGGTC CAATGTGTGA CTTGCTGTGG 
    TCAGATCCAG ATGACCGTGG TGGTTGGGGT ATATCTCCTC GAGGAGCTGG TTACACCTTT 
    GGGCAAGATA TTTCTGAGAC ATTTAATCAT GCCAATGGCC TCACGTTGGT GTCTAGAGCT 
    CACCAGCTAG TGATGGAGGG ATATAACTGG TGCCATGACC GGAATGTAGT AACGATTTTC 
    AGTGCTCCAA ACTATTGTTA TCGTTGTGGT AACCAAGCTG CAATCATGGA ACTTGACGAT 
    ACTCTAAAAT ACTCTTTCTT GCAGTTTGAC CCAGCACCTC GTAGAGGCGA GCCACATGTT 
    ACTCGTCGTA CCCCAGACTA CTTCCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    PPP2CA ( NM_002715.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002706.1 Homo sapiens protein phosphatase 2 catalytic subunit alpha (PPP2CA), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu20615
    Clone ID Related Accession (Same CDS sequence) NM_002715.3 , NM_002715.4 , NM_002715.2
    Accession Version NM_002715.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 930bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104109.3 and AK097599.1. On Nov 23, 2018 this sequence version replaced NM_002715.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.67081.1, SRR1660803.123457.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820687, SAMN03267764 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000481195.6/ ENSP00000418447.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGACGAGA AGGTGTTCAC CAAGGAGCTG GACCAGTGGA TCGAGCAGCT GAACGAGTGC 
    AAGCAGCTGT CCGAGTCCCA GGTCAAGAGC CTCTGCGAGA AGGCTAAAGA AATCCTGACA 
    AAAGAATCCA ACGTGCAAGA GGTTCGATGT CCAGTTACTG TCTGTGGAGA TGTGCATGGG 
    CAATTTCATG ATCTCATGGA ACTGTTTAGA ATTGGTGGCA AATCACCAGA TACAAATTAC 
    TTGTTTATGG GAGATTATGT TGACAGAGGA TATTATTCAG TTGAAACAGT TACACTGCTT 
    GTAGCTCTTA AGGTTCGTTA CCGTGAACGC ATCACCATTC TTCGAGGGAA TCATGAGAGC 
    AGACAGATCA CACAAGTTTA TGGTTTCTAT GATGAATGTT TAAGAAAATA TGGAAATGCA 
    AATGTTTGGA AATATTTTAC AGATCTTTTT GACTATCTTC CTCTCACTGC CTTGGTGGAT 
    GGGCAGATCT TCTGTCTACA TGGTGGTCTC TCGCCATCTA TAGATACACT GGATCATATC 
    AGAGCACTTG ATCGCCTACA AGAAGTTCCC CATGAGGGTC CAATGTGTGA CTTGCTGTGG 
    TCAGATCCAG ATGACCGTGG TGGTTGGGGT ATATCTCCTC GAGGAGCTGG TTACACCTTT 
    GGGCAAGATA TTTCTGAGAC ATTTAATCAT GCCAATGGCC TCACGTTGGT GTCTAGAGCT 
    CACCAGCTAG TGATGGAGGG ATATAACTGG TGCCATGACC GGAATGTAGT AACGATTTTC 
    AGTGCTCCAA ACTATTGTTA TCGTTGTGGT AACCAAGCTG CAATCATGGA ACTTGACGAT 
    ACTCTAAAAT ACTCTTTCTT GCAGTTTGAC CCAGCACCTC GTAGAGGCGA GCCACATGTT 
    ACTCGTCGTA CCCCAGACTA CTTCCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002706.1
    CDS213..1142
    Translation

    Target ORF information:

    RefSeq Version NM_002715.4
    Organism Homo sapiens(human)
    Definition Homo sapiens protein phosphatase 2 catalytic subunit alpha (PPP2CA), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_002715.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGACGAGA AGGTGTTCAC CAAGGAGCTG GACCAGTGGA TCGAGCAGCT GAACGAGTGC 
    AAGCAGCTGT CCGAGTCCCA GGTCAAGAGC CTCTGCGAGA AGGCTAAAGA AATCCTGACA 
    AAAGAATCCA ACGTGCAAGA GGTTCGATGT CCAGTTACTG TCTGTGGAGA TGTGCATGGG 
    CAATTTCATG ATCTCATGGA ACTGTTTAGA ATTGGTGGCA AATCACCAGA TACAAATTAC 
    TTGTTTATGG GAGATTATGT TGACAGAGGA TATTATTCAG TTGAAACAGT TACACTGCTT 
    GTAGCTCTTA AGGTTCGTTA CCGTGAACGC ATCACCATTC TTCGAGGGAA TCATGAGAGC 
    AGACAGATCA CACAAGTTTA TGGTTTCTAT GATGAATGTT TAAGAAAATA TGGAAATGCA 
    AATGTTTGGA AATATTTTAC AGATCTTTTT GACTATCTTC CTCTCACTGC CTTGGTGGAT 
    GGGCAGATCT TCTGTCTACA TGGTGGTCTC TCGCCATCTA TAGATACACT GGATCATATC 
    AGAGCACTTG ATCGCCTACA AGAAGTTCCC CATGAGGGTC CAATGTGTGA CTTGCTGTGG 
    TCAGATCCAG ATGACCGTGG TGGTTGGGGT ATATCTCCTC GAGGAGCTGG TTACACCTTT 
    GGGCAAGATA TTTCTGAGAC ATTTAATCAT GCCAATGGCC TCACGTTGGT GTCTAGAGCT 
    CACCAGCTAG TGATGGAGGG ATATAACTGG TGCCATGACC GGAATGTAGT AACGATTTTC 
    AGTGCTCCAA ACTATTGTTA TCGTTGTGGT AACCAAGCTG CAATCATGGA ACTTGACGAT 
    ACTCTAAAAT ACTCTTTCTT GCAGTTTGAC CCAGCACCTC GTAGAGGCGA GCCACATGTT 
    ACTCGTCGTA CCCCAGACTA CTTCCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    PPP2CA ( NM_002715.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_002706.1 Homo sapiens protein phosphatase 2 catalytic subunit alpha (PPP2CA), mRNA.
    Price & Availability↑
    CloneID OHu20615
    Clone ID Related Accession (Same CDS sequence) NM_002715.3 , NM_002715.4 , NM_002715.2
    Accession Version NM_002715.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 930bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-12
    Organism Homo sapiens(Human)
    Product serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
    Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AV705728.1, AC104109.3, AK097599.1 and BM786995.1. This sequence is a reference standard in the RefSeqGene project. On Jan 7, 2005 this sequence version replaced NM_002715.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK097599.1, BC002657.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820687, SAMN03267764 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGACGAGA AGGTGTTCAC CAAGGAGCTG GACCAGTGGA TCGAGCAGCT GAACGAGTGC 
    AAGCAGCTGT CCGAGTCCCA GGTCAAGAGC CTCTGCGAGA AGGCTAAAGA AATCCTGACA 
    AAAGAATCCA ACGTGCAAGA GGTTCGATGT CCAGTTACTG TCTGTGGAGA TGTGCATGGG 
    CAATTTCATG ATCTCATGGA ACTGTTTAGA ATTGGTGGCA AATCACCAGA TACAAATTAC 
    TTGTTTATGG GAGATTATGT TGACAGAGGA TATTATTCAG TTGAAACAGT TACACTGCTT 
    GTAGCTCTTA AGGTTCGTTA CCGTGAACGC ATCACCATTC TTCGAGGGAA TCATGAGAGC 
    AGACAGATCA CACAAGTTTA TGGTTTCTAT GATGAATGTT TAAGAAAATA TGGAAATGCA 
    AATGTTTGGA AATATTTTAC AGATCTTTTT GACTATCTTC CTCTCACTGC CTTGGTGGAT 
    GGGCAGATCT TCTGTCTACA TGGTGGTCTC TCGCCATCTA TAGATACACT GGATCATATC 
    AGAGCACTTG ATCGCCTACA AGAAGTTCCC CATGAGGGTC CAATGTGTGA CTTGCTGTGG 
    TCAGATCCAG ATGACCGTGG TGGTTGGGGT ATATCTCCTC GAGGAGCTGG TTACACCTTT 
    GGGCAAGATA TTTCTGAGAC ATTTAATCAT GCCAATGGCC TCACGTTGGT GTCTAGAGCT 
    CACCAGCTAG TGATGGAGGG ATATAACTGG TGCCATGACC GGAATGTAGT AACGATTTTC 
    AGTGCTCCAA ACTATTGTTA TCGTTGTGGT AACCAAGCTG CAATCATGGA ACTTGACGAT 
    ACTCTAAAAT ACTCTTTCTT GCAGTTTGAC CCAGCACCTC GTAGAGGCGA GCCACATGTT 
    ACTCGTCGTA CCCCAGACTA CTTCCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002706.1
    CDS399..1328
    Translation

    Target ORF information:

    RefSeq Version NM_002715.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens protein phosphatase 2 catalytic subunit alpha (PPP2CA), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_002715.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGACGAGA AGGTGTTCAC CAAGGAGCTG GACCAGTGGA TCGAGCAGCT GAACGAGTGC 
    AAGCAGCTGT CCGAGTCCCA GGTCAAGAGC CTCTGCGAGA AGGCTAAAGA AATCCTGACA 
    AAAGAATCCA ACGTGCAAGA GGTTCGATGT CCAGTTACTG TCTGTGGAGA TGTGCATGGG 
    CAATTTCATG ATCTCATGGA ACTGTTTAGA ATTGGTGGCA AATCACCAGA TACAAATTAC 
    TTGTTTATGG GAGATTATGT TGACAGAGGA TATTATTCAG TTGAAACAGT TACACTGCTT 
    GTAGCTCTTA AGGTTCGTTA CCGTGAACGC ATCACCATTC TTCGAGGGAA TCATGAGAGC 
    AGACAGATCA CACAAGTTTA TGGTTTCTAT GATGAATGTT TAAGAAAATA TGGAAATGCA 
    AATGTTTGGA AATATTTTAC AGATCTTTTT GACTATCTTC CTCTCACTGC CTTGGTGGAT 
    GGGCAGATCT TCTGTCTACA TGGTGGTCTC TCGCCATCTA TAGATACACT GGATCATATC 
    AGAGCACTTG ATCGCCTACA AGAAGTTCCC CATGAGGGTC CAATGTGTGA CTTGCTGTGG 
    TCAGATCCAG ATGACCGTGG TGGTTGGGGT ATATCTCCTC GAGGAGCTGG TTACACCTTT 
    GGGCAAGATA TTTCTGAGAC ATTTAATCAT GCCAATGGCC TCACGTTGGT GTCTAGAGCT 
    CACCAGCTAG TGATGGAGGG ATATAACTGG TGCCATGACC GGAATGTAGT AACGATTTTC 
    AGTGCTCCAA ACTATTGTTA TCGTTGTGGT AACCAAGCTG CAATCATGGA ACTTGACGAT 
    ACTCTAAAAT ACTCTTTCTT GCAGTTTGAC CCAGCACCTC GTAGAGGCGA GCCACATGTT 
    ACTCGTCGTA CCCCAGACTA CTTCCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    PPP2CA ( NM_001355019.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341948.1 Homo sapiens protein phosphatase 2 catalytic subunit alpha (PPP2CA), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu102930
    Clone ID Related Accession (Same CDS sequence) NM_001355019.1
    Accession Version NM_001355019.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 735bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-29
    Organism Homo sapiens(human)
    Product serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104109.3. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.13549.1, SRR1803611.62992.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820687, SAMN03267764 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGGAACTGT TTAGAATTGG TGGCAAATCA CCAGATACAA ATTACTTGTT TATGGGAGAT 
    TATGTTGACA GAGGATATTA TTCAGTTGAA ACAGTTACAC TGCTTGTAGC TCTTAAGGTT 
    CGTTACCGTG AACGCATCAC CATTCTTCGA GGGAATCATG AGAGCAGACA GATCACACAA 
    GTTTATGGTT TCTATGATGA ATGTTTAAGA AAATATGGAA ATGCAAATGT TTGGAAATAT 
    TTTACAGATC TTTTTGACTA TCTTCCTCTC ACTGCCTTGG TGGATGGGCA GATCTTCTGT 
    CTACATGGTG GTCTCTCGCC ATCTATAGAT ACACTGGATC ATATCAGAGC ACTTGATCGC 
    CTACAAGAAG TTCCCCATGA GGGTCCAATG TGTGACTTGC TGTGGTCAGA TCCAGATGAC 
    CGTGGTGGTT GGGGTATATC TCCTCGAGGA GCTGGTTACA CCTTTGGGCA AGATATTTCT 
    GAGACATTTA ATCATGCCAA TGGCCTCACG TTGGTGTCTA GAGCTCACCA GCTAGTGATG 
    GAGGGATATA ACTGGTGCCA TGACCGGAAT GTAGTAACGA TTTTCAGTGC TCCAAACTAT 
    TGTTATCGTT GTGGTAACCA AGCTGCAATC ATGGAACTTG ACGATACTCT AAAATACTCT 
    TTCTTGCAGT TTGACCCAGC ACCTCGTAGA GGCGAGCCAC ATGTTACTCG TCGTACCCCA 
    GACTACTTCC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341948.1
    CDS440..1174
    Translation

    Target ORF information:

    RefSeq Version NM_001355019.1
    Organism Homo sapiens(human)
    Definition Homo sapiens protein phosphatase 2 catalytic subunit alpha (PPP2CA), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001355019.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGGAACTGT TTAGAATTGG TGGCAAATCA CCAGATACAA ATTACTTGTT TATGGGAGAT 
    TATGTTGACA GAGGATATTA TTCAGTTGAA ACAGTTACAC TGCTTGTAGC TCTTAAGGTT 
    CGTTACCGTG AACGCATCAC CATTCTTCGA GGGAATCATG AGAGCAGACA GATCACACAA 
    GTTTATGGTT TCTATGATGA ATGTTTAAGA AAATATGGAA ATGCAAATGT TTGGAAATAT 
    TTTACAGATC TTTTTGACTA TCTTCCTCTC ACTGCCTTGG TGGATGGGCA GATCTTCTGT 
    CTACATGGTG GTCTCTCGCC ATCTATAGAT ACACTGGATC ATATCAGAGC ACTTGATCGC 
    CTACAAGAAG TTCCCCATGA GGGTCCAATG TGTGACTTGC TGTGGTCAGA TCCAGATGAC 
    CGTGGTGGTT GGGGTATATC TCCTCGAGGA GCTGGTTACA CCTTTGGGCA AGATATTTCT 
    GAGACATTTA ATCATGCCAA TGGCCTCACG TTGGTGTCTA GAGCTCACCA GCTAGTGATG 
    GAGGGATATA ACTGGTGCCA TGACCGGAAT GTAGTAACGA TTTTCAGTGC TCCAAACTAT 
    TGTTATCGTT GTGGTAACCA AGCTGCAATC ATGGAACTTG ACGATACTCT AAAATACTCT 
    TTCTTGCAGT TTGACCCAGC ACCTCGTAGA GGCGAGCCAC ATGTTACTCG TCGTACCCCA 
    GACTACTTCC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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