CDCA8 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CDCA8
Entrez Gene ID	55143
Full Name	cell division cycle associated 8
Synonyms	BOR,BOREALIN,DasraB,MESRGP
General protein information	Preferred Names cell division cycle associated 8 Names borealin Dasra B cell division cycle-associated protein 8 dasra-B hDasra-B pluripotent embryonic stem cell-related gene 3 protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p34.3
Summary	This gene encodes a component of the chromosomal passenger complex. This complex is an essential regulator of mitosis and cell division. This protein is cell-cycle regulated and is required for chromatin-induced microtubule stabilization and spindle formation. Alternate splicing results in multiple transcript variants. Pseudgenes of this gene are found on chromosomes 7, 8 and 16. [provided by RefSeq, Apr 2013].
Disorder MIM:	609977

mRNA and Protein(s)

mRNA	Protein	Name
NM_001256875.1	NP_001243804.1	borealin
NM_018101.4	NP_060571.1	borealin
NM_018101.3	NP_060571.1	borealin
NM_001256875.2	NP_001243804.1	borealin

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CDCA8	NP_001233359.1
	CDCA8	XP_001115347.1
Rattus norvegicus (Norway rat)	Cdca8	NP_001020221.1
Xenopus tropicalis (tropical clawed frog)	cdca8	NP_001002902.1
Homo sapiens (human)	CDCA8	NP_001243804.1
Canis lupus familiaris (dog)	CDCA8	XP_850190.1
Mus musculus (house mouse)	Cdca8	NP_080836.3
Bos taurus (cattle)	CDCA8	NP_001077121.1
Danio rerio (zebrafish)	cdca8	NP_001007457.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in BOREALIN cause thyroid dysgenesis.
Carr? A, Stoupa A, Kariyawasam D, Gueriouz M, Ramond C, Monus T, L?ger J, Gaujoux S, Sebag F, Glaser N, Zenaty D, Nitschke P, Bole-Feysot C, Hubert L, Lyonnet S, Scharfmann R, Munnich A, Besmond C, Taylor W, Polak M
Human molecular genetics26(3)599-610(2017 02)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results were well correlated with the same gene expression pattern analysed in the thyroid tissue of the patient with BOREALIN-p.R114W. These studies open new avenues in the genetics of TD in humans.
Title: Mutations in BOREALIN cause thyroid dysgenesis.

enhanced CDCA8 promoter activities by NF-Y overexpression and reduced CDCA8 transcription by NF-Y knockdown further verified that NF-Y is a positive regulator of CDCA8 transcription.
Title: Transcriptional activation of human CDCA8 gene regulated by transcription factor NF-Y in embryonic stem cells and cancer cells.

Borealin dimerization mediates chromosomal passenger complex checkpoint function by enhancing localization to centromeres and kinetochores.
Title: Borealin dimerization mediates optimal CPC checkpoint function by enhancing localization to centromeres and kinetochores.

In colorectal cancer patients, Borealin expression was positively correlated with age, lymph node metastasis and neoplasm staging.
Title: Borealin/Dasra B is overexpressed in colorectal cancers and contributes to proliferation of cancer cells.

These findings reveal a previously unrecognized but direct link between HP1 and CPC localization in the centromere and illustrate the critical role of borealin-HP1 interaction in orchestrating an accurate cell division.
Title: Chromatin protein HP1 interacts with the mitotic regulator borealin protein and specifies the centromere localization of the chromosomal passenger complex.

The following CDCA8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDCA8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10906	NM_018101.4 Latest version!	Homo sapiens cell division cycle associated 8 (CDCA8), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu10906	NM_001256875.2 Latest version!	Homo sapiens cell division cycle associated 8 (CDCA8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu10906	NM_018101.3	Homo sapiens cell division cycle associated 8 (CDCA8), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu10906	NM_001256875.1	Homo sapiens cell division cycle associated 8 (CDCA8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CDCA8 ( NM_001256875.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243804.1 Homo sapiens cell division cycle associated 8 (CDCA8), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu10906
Clone ID Related Accession (Same CDS sequence)	NM_001256875.1 , NM_018101.4 , NM_018101.3 , NM_001256875.2
Accession Version	NM_001256875.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-17
Organism	Homo sapiens(human)
Product	borealin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104336.2, BC001651.2 and BC008079.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.651233.1, BC000703.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCCTA GGAAGGGCAG TAGTCGGGTG GCCAAGACCA ACTCCTTACG GAGGCGGAAG 
CTCGCCTCCT TTCTGAAAGA CTTCGACCGT GAAGTGGAAA TACGAATCAA GCAAATTGAG 
TCAGACAGGC AGAACCTCCT CAAGGAGGTG GATAACCTCT ACAACATCGA GATCCTGCGG 
CTCCCCAAGG CTCTGCGCGA GATGAACTGG CTTGACTACT TCGCCCTTGG AGGAAACAAA 
CAGGCCCTGG AAGAGGCGGC AACAGCTGAC CTGGATATCA CCGAAATAAA CAAACTAACA 
GCAGAAGCTA TTCAGACACC CCTGAAATCT GCCAAAACAC GAAAGGTAAT ACAGGTAGAT 
GAAATGATAG TGGAAGAGGA AGAAGAAGAA GAAAATGAAC GTAAGAATCT TCAAACTGCA 
AGAGTCAAAA GGTGTCCTCC ATCCAAGAAG AGAACTCAGT CCATACAAGG AAAAGGAAAA 
GGGAAAAGGT CAAGCCGTGC TAACACTGTT ACCCCAGCCG TGGGCCGATT GGAGGTGTCC 
ATGGTCAAAC CAACTCCAGG CCTGACACCC AGGTTTGACT CAAGGGTCTT CAAGACCCCT 
GGCCTGCGTA CTCCAGCAGC AGGAGAGCGG ATTTACAACA TCTCAGGGAA TGGCAGCCCT 
CTTGCTGACA GCAAAGAGAT CTTCCTCACT GTGCCAGTGG GCGGCGGAGA GAGCCTGCGA 
TTATTGGCCA GTGACTTGCA GAGGCACAGT ATTGCCCAGC TGGATCCAGA GGCCTTGGGA 
AACATTAAGA AGCTCTCCAA CCGTCTCGCC CAAATCTGCA GCAGCATACG GACCCACAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243804.1
CDS	291..1133
Translation	MAPRKGSSRVAKTNSLRRRKLASFLKDFDREVEIRIKQIESDRQNLLKEVDNLYNIEILRLPKALREMNWLDYFALGGNKQALEEAATADLDITEINKLTAEAIQTPLKSAKTRKVIQVDEMIVEEEEEEENERKNLQTARVKRCPPSKKRTQSIQGKGKGKRSSRANTVTPAVGRLEVSMVKPTPGLTPRFDSRVFKTPGLRTPAAGERIYNISGNGSPLADSKEIFLTVPVGGGESLRLLASDLQRHSIAQLDPEALGNIKKLSNRLAQICSSIRTHK

Target ORF information:

RefSeq Version	NM_001256875.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle associated 8 (CDCA8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256875.1

ORF Insert Sequence:

ATGGCTCCTA GGAAGGGCAG TAGTCGGGTG GCCAAGACCA ACTCCTTACG GAGGCGGAAG 
CTCGCCTCCT TTCTGAAAGA CTTCGACCGT GAAGTGGAAA TACGAATCAA GCAAATTGAG 
TCAGACAGGC AGAACCTCCT CAAGGAGGTG GATAACCTCT ACAACATCGA GATCCTGCGG 
CTCCCCAAGG CTCTGCGCGA GATGAACTGG CTTGACTACT TCGCCCTTGG AGGAAACAAA 
CAGGCCCTGG AAGAGGCGGC AACAGCTGAC CTGGATATCA CCGAAATAAA CAAACTAACA 
GCAGAAGCTA TTCAGACACC CCTGAAATCT GCCAAAACAC GAAAGGTAAT ACAGGTAGAT 
GAAATGATAG TGGAAGAGGA AGAAGAAGAA GAAAATGAAC GTAAGAATCT TCAAACTGCA 
AGAGTCAAAA GGTGTCCTCC ATCCAAGAAG AGAACTCAGT CCATACAAGG AAAAGGAAAA 
GGGAAAAGGT CAAGCCGTGC TAACACTGTT ACCCCAGCCG TGGGCCGATT GGAGGTGTCC 
ATGGTCAAAC CAACTCCAGG CCTGACACCC AGGTTTGACT CAAGGGTCTT CAAGACCCCT 
GGCCTGCGTA CTCCAGCAGC AGGAGAGCGG ATTTACAACA TCTCAGGGAA TGGCAGCCCT 
CTTGCTGACA GCAAAGAGAT CTTCCTCACT GTGCCAGTGG GCGGCGGAGA GAGCCTGCGA 
TTATTGGCCA GTGACTTGCA GAGGCACAGT ATTGCCCAGC TGGATCCAGA GGCCTTGGGA 
AACATTAAGA AGCTCTCCAA CCGTCTCGCC CAAATCTGCA GCAGCATACG GACCCACAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDCA8 ( NM_018101.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060571.1 Homo sapiens cell division cycle associated 8 (CDCA8), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10906
Clone ID Related Accession (Same CDS sequence)	NM_001256875.1 , NM_018101.4 , NM_018101.3 , NM_001256875.2
Accession Version	NM_018101.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	borealin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY508815.2 and BC008079.2. On May 31, 2019 this sequence version replaced NM_018101.3. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC008079.2, AY508815.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCCTA GGAAGGGCAG TAGTCGGGTG GCCAAGACCA ACTCCTTACG GAGGCGGAAG 
CTCGCCTCCT TTCTGAAAGA CTTCGACCGT GAAGTGGAAA TACGAATCAA GCAAATTGAG 
TCAGACAGGC AGAACCTCCT CAAGGAGGTG GATAACCTCT ACAACATCGA GATCCTGCGG 
CTCCCCAAGG CTCTGCGCGA GATGAACTGG CTTGACTACT TCGCCCTTGG AGGAAACAAA 
CAGGCCCTGG AAGAGGCGGC AACAGCTGAC CTGGATATCA CCGAAATAAA CAAACTAACA 
GCAGAAGCTA TTCAGACACC CCTGAAATCT GCCAAAACAC GAAAGGTAAT ACAGGTAGAT 
GAAATGATAG TGGAAGAGGA AGAAGAAGAA GAAAATGAAC GTAAGAATCT TCAAACTGCA 
AGAGTCAAAA GGTGTCCTCC ATCCAAGAAG AGAACTCAGT CCATACAAGG AAAAGGAAAA 
GGGAAAAGGT CAAGCCGTGC TAACACTGTT ACCCCAGCCG TGGGCCGATT GGAGGTGTCC 
ATGGTCAAAC CAACTCCAGG CCTGACACCC AGGTTTGACT CAAGGGTCTT CAAGACCCCT 
GGCCTGCGTA CTCCAGCAGC AGGAGAGCGG ATTTACAACA TCTCAGGGAA TGGCAGCCCT 
CTTGCTGACA GCAAAGAGAT CTTCCTCACT GTGCCAGTGG GCGGCGGAGA GAGCCTGCGA 
TTATTGGCCA GTGACTTGCA GAGGCACAGT ATTGCCCAGC TGGATCCAGA GGCCTTGGGA 
AACATTAAGA AGCTCTCCAA CCGTCTCGCC CAAATCTGCA GCAGCATACG GACCCACAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060571.1
CDS	79..921
Translation	MAPRKGSSRVAKTNSLRRRKLASFLKDFDREVEIRIKQIESDRQNLLKEVDNLYNIEILRLPKALREMNWLDYFALGGNKQALEEAATADLDITEINKLTAEAIQTPLKSAKTRKVIQVDEMIVEEEEEEENERKNLQTARVKRCPPSKKRTQSIQGKGKGKRSSRANTVTPAVGRLEVSMVKPTPGLTPRFDSRVFKTPGLRTPAAGERIYNISGNGSPLADSKEIFLTVPVGGGESLRLLASDLQRHSIAQLDPEALGNIKKLSNRLAQICSSIRTHK

Target ORF information:

RefSeq Version	NM_018101.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle associated 8 (CDCA8), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018101.4

ORF Insert Sequence:

ATGGCTCCTA GGAAGGGCAG TAGTCGGGTG GCCAAGACCA ACTCCTTACG GAGGCGGAAG 
CTCGCCTCCT TTCTGAAAGA CTTCGACCGT GAAGTGGAAA TACGAATCAA GCAAATTGAG 
TCAGACAGGC AGAACCTCCT CAAGGAGGTG GATAACCTCT ACAACATCGA GATCCTGCGG 
CTCCCCAAGG CTCTGCGCGA GATGAACTGG CTTGACTACT TCGCCCTTGG AGGAAACAAA 
CAGGCCCTGG AAGAGGCGGC AACAGCTGAC CTGGATATCA CCGAAATAAA CAAACTAACA 
GCAGAAGCTA TTCAGACACC CCTGAAATCT GCCAAAACAC GAAAGGTAAT ACAGGTAGAT 
GAAATGATAG TGGAAGAGGA AGAAGAAGAA GAAAATGAAC GTAAGAATCT TCAAACTGCA 
AGAGTCAAAA GGTGTCCTCC ATCCAAGAAG AGAACTCAGT CCATACAAGG AAAAGGAAAA 
GGGAAAAGGT CAAGCCGTGC TAACACTGTT ACCCCAGCCG TGGGCCGATT GGAGGTGTCC 
ATGGTCAAAC CAACTCCAGG CCTGACACCC AGGTTTGACT CAAGGGTCTT CAAGACCCCT 
GGCCTGCGTA CTCCAGCAGC AGGAGAGCGG ATTTACAACA TCTCAGGGAA TGGCAGCCCT 
CTTGCTGACA GCAAAGAGAT CTTCCTCACT GTGCCAGTGG GCGGCGGAGA GAGCCTGCGA 
TTATTGGCCA GTGACTTGCA GAGGCACAGT ATTGCCCAGC TGGATCCAGA GGCCTTGGGA 
AACATTAAGA AGCTCTCCAA CCGTCTCGCC CAAATCTGCA GCAGCATACG GACCCACAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDCA8 ( NM_018101.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060571.1 Homo sapiens cell division cycle associated 8 (CDCA8), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10906
Clone ID Related Accession (Same CDS sequence)	NM_001256875.1 , NM_018101.4 , NM_018101.3 , NM_001256875.2
Accession Version	NM_018101.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	borealin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104336.2, AY508815.2 and BC008079.2. On Mar 3, 2012 this sequence version replaced NM_018101.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC008079.2, AK001330.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCCTA GGAAGGGCAG TAGTCGGGTG GCCAAGACCA ACTCCTTACG GAGGCGGAAG 
CTCGCCTCCT TTCTGAAAGA CTTCGACCGT GAAGTGGAAA TACGAATCAA GCAAATTGAG 
TCAGACAGGC AGAACCTCCT CAAGGAGGTG GATAACCTCT ACAACATCGA GATCCTGCGG 
CTCCCCAAGG CTCTGCGCGA GATGAACTGG CTTGACTACT TCGCCCTTGG AGGAAACAAA 
CAGGCCCTGG AAGAGGCGGC AACAGCTGAC CTGGATATCA CCGAAATAAA CAAACTAACA 
GCAGAAGCTA TTCAGACACC CCTGAAATCT GCCAAAACAC GAAAGGTAAT ACAGGTAGAT 
GAAATGATAG TGGAAGAGGA AGAAGAAGAA GAAAATGAAC GTAAGAATCT TCAAACTGCA 
AGAGTCAAAA GGTGTCCTCC ATCCAAGAAG AGAACTCAGT CCATACAAGG AAAAGGAAAA 
GGGAAAAGGT CAAGCCGTGC TAACACTGTT ACCCCAGCCG TGGGCCGATT GGAGGTGTCC 
ATGGTCAAAC CAACTCCAGG CCTGACACCC AGGTTTGACT CAAGGGTCTT CAAGACCCCT 
GGCCTGCGTA CTCCAGCAGC AGGAGAGCGG ATTTACAACA TCTCAGGGAA TGGCAGCCCT 
CTTGCTGACA GCAAAGAGAT CTTCCTCACT GTGCCAGTGG GCGGCGGAGA GAGCCTGCGA 
TTATTGGCCA GTGACTTGCA GAGGCACAGT ATTGCCCAGC TGGATCCAGA GGCCTTGGGA 
AACATTAAGA AGCTCTCCAA CCGTCTCGCC CAAATCTGCA GCAGCATACG GACCCACAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060571.1
CDS	194..1036
Translation	MAPRKGSSRVAKTNSLRRRKLASFLKDFDREVEIRIKQIESDRQNLLKEVDNLYNIEILRLPKALREMNWLDYFALGGNKQALEEAATADLDITEINKLTAEAIQTPLKSAKTRKVIQVDEMIVEEEEEEENERKNLQTARVKRCPPSKKRTQSIQGKGKGKRSSRANTVTPAVGRLEVSMVKPTPGLTPRFDSRVFKTPGLRTPAAGERIYNISGNGSPLADSKEIFLTVPVGGGESLRLLASDLQRHSIAQLDPEALGNIKKLSNRLAQICSSIRTHK

Target ORF information:

RefSeq Version	NM_018101.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle associated 8 (CDCA8), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018101.3

ORF Insert Sequence:

ATGGCTCCTA GGAAGGGCAG TAGTCGGGTG GCCAAGACCA ACTCCTTACG GAGGCGGAAG 
CTCGCCTCCT TTCTGAAAGA CTTCGACCGT GAAGTGGAAA TACGAATCAA GCAAATTGAG 
TCAGACAGGC AGAACCTCCT CAAGGAGGTG GATAACCTCT ACAACATCGA GATCCTGCGG 
CTCCCCAAGG CTCTGCGCGA GATGAACTGG CTTGACTACT TCGCCCTTGG AGGAAACAAA 
CAGGCCCTGG AAGAGGCGGC AACAGCTGAC CTGGATATCA CCGAAATAAA CAAACTAACA 
GCAGAAGCTA TTCAGACACC CCTGAAATCT GCCAAAACAC GAAAGGTAAT ACAGGTAGAT 
GAAATGATAG TGGAAGAGGA AGAAGAAGAA GAAAATGAAC GTAAGAATCT TCAAACTGCA 
AGAGTCAAAA GGTGTCCTCC ATCCAAGAAG AGAACTCAGT CCATACAAGG AAAAGGAAAA 
GGGAAAAGGT CAAGCCGTGC TAACACTGTT ACCCCAGCCG TGGGCCGATT GGAGGTGTCC 
ATGGTCAAAC CAACTCCAGG CCTGACACCC AGGTTTGACT CAAGGGTCTT CAAGACCCCT 
GGCCTGCGTA CTCCAGCAGC AGGAGAGCGG ATTTACAACA TCTCAGGGAA TGGCAGCCCT 
CTTGCTGACA GCAAAGAGAT CTTCCTCACT GTGCCAGTGG GCGGCGGAGA GAGCCTGCGA 
TTATTGGCCA GTGACTTGCA GAGGCACAGT ATTGCCCAGC TGGATCCAGA GGCCTTGGGA 
AACATTAAGA AGCTCTCCAA CCGTCTCGCC CAAATCTGCA GCAGCATACG GACCCACAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDCA8 ( NM_001256875.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243804.1 Homo sapiens cell division cycle associated 8 (CDCA8), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu10906
Clone ID Related Accession (Same CDS sequence)	NM_001256875.1 , NM_018101.4 , NM_018101.3 , NM_001256875.2
Accession Version	NM_001256875.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	borealin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC001651.2 and BC008079.2. On Nov 23, 2018 this sequence version replaced NM_001256875.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.651233.1, BC000703.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000373055.6/ ENSP00000362146.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTCCTA GGAAGGGCAG TAGTCGGGTG GCCAAGACCA ACTCCTTACG GAGGCGGAAG 
CTCGCCTCCT TTCTGAAAGA CTTCGACCGT GAAGTGGAAA TACGAATCAA GCAAATTGAG 
TCAGACAGGC AGAACCTCCT CAAGGAGGTG GATAACCTCT ACAACATCGA GATCCTGCGG 
CTCCCCAAGG CTCTGCGCGA GATGAACTGG CTTGACTACT TCGCCCTTGG AGGAAACAAA 
CAGGCCCTGG AAGAGGCGGC AACAGCTGAC CTGGATATCA CCGAAATAAA CAAACTAACA 
GCAGAAGCTA TTCAGACACC CCTGAAATCT GCCAAAACAC GAAAGGTAAT ACAGGTAGAT 
GAAATGATAG TGGAAGAGGA AGAAGAAGAA GAAAATGAAC GTAAGAATCT TCAAACTGCA 
AGAGTCAAAA GGTGTCCTCC ATCCAAGAAG AGAACTCAGT CCATACAAGG AAAAGGAAAA 
GGGAAAAGGT CAAGCCGTGC TAACACTGTT ACCCCAGCCG TGGGCCGATT GGAGGTGTCC 
ATGGTCAAAC CAACTCCAGG CCTGACACCC AGGTTTGACT CAAGGGTCTT CAAGACCCCT 
GGCCTGCGTA CTCCAGCAGC AGGAGAGCGG ATTTACAACA TCTCAGGGAA TGGCAGCCCT 
CTTGCTGACA GCAAAGAGAT CTTCCTCACT GTGCCAGTGG GCGGCGGAGA GAGCCTGCGA 
TTATTGGCCA GTGACTTGCA GAGGCACAGT ATTGCCCAGC TGGATCCAGA GGCCTTGGGA 
AACATTAAGA AGCTCTCCAA CCGTCTCGCC CAAATCTGCA GCAGCATACG GACCCACAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243804.1
CDS	176..1018
Translation	MAPRKGSSRVAKTNSLRRRKLASFLKDFDREVEIRIKQIESDRQNLLKEVDNLYNIEILRLPKALREMNWLDYFALGGNKQALEEAATADLDITEINKLTAEAIQTPLKSAKTRKVIQVDEMIVEEEEEEENERKNLQTARVKRCPPSKKRTQSIQGKGKGKRSSRANTVTPAVGRLEVSMVKPTPGLTPRFDSRVFKTPGLRTPAAGERIYNISGNGSPLADSKEIFLTVPVGGGESLRLLASDLQRHSIAQLDPEALGNIKKLSNRLAQICSSIRTHK

Target ORF information:

RefSeq Version	NM_001256875.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle associated 8 (CDCA8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256875.2

ORF Insert Sequence:

ATGGCTCCTA GGAAGGGCAG TAGTCGGGTG GCCAAGACCA ACTCCTTACG GAGGCGGAAG 
CTCGCCTCCT TTCTGAAAGA CTTCGACCGT GAAGTGGAAA TACGAATCAA GCAAATTGAG 
TCAGACAGGC AGAACCTCCT CAAGGAGGTG GATAACCTCT ACAACATCGA GATCCTGCGG 
CTCCCCAAGG CTCTGCGCGA GATGAACTGG CTTGACTACT TCGCCCTTGG AGGAAACAAA 
CAGGCCCTGG AAGAGGCGGC AACAGCTGAC CTGGATATCA CCGAAATAAA CAAACTAACA 
GCAGAAGCTA TTCAGACACC CCTGAAATCT GCCAAAACAC GAAAGGTAAT ACAGGTAGAT 
GAAATGATAG TGGAAGAGGA AGAAGAAGAA GAAAATGAAC GTAAGAATCT TCAAACTGCA 
AGAGTCAAAA GGTGTCCTCC ATCCAAGAAG AGAACTCAGT CCATACAAGG AAAAGGAAAA 
GGGAAAAGGT CAAGCCGTGC TAACACTGTT ACCCCAGCCG TGGGCCGATT GGAGGTGTCC 
ATGGTCAAAC CAACTCCAGG CCTGACACCC AGGTTTGACT CAAGGGTCTT CAAGACCCCT 
GGCCTGCGTA CTCCAGCAGC AGGAGAGCGG ATTTACAACA TCTCAGGGAA TGGCAGCCCT 
CTTGCTGACA GCAAAGAGAT CTTCCTCACT GTGCCAGTGG GCGGCGGAGA GAGCCTGCGA 
TTATTGGCCA GTGACTTGCA GAGGCACAGT ATTGCCCAGC TGGATCCAGA GGCCTTGGGA 
AACATTAAGA AGCTCTCCAA CCGTCTCGCC CAAATCTGCA GCAGCATACG GACCCACAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations in BOREALIN cause thyroid dysgenesis.
Human molecular genetics26(3)599-610(2017 02)
Carr? A,Stoupa A,Kariyawasam D,Gueriouz M,Ramond C,Monus T,L?ger J,Gaujoux S,Sebag F,Glaser N,Zenaty D,Nitschke P,Bole-Feysot C,Hubert L,Lyonnet S,Scharfmann R,Munnich A,Besmond C,Taylor W,Polak M

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CDCA8 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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