ATG2B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATG2B
Entrez Gene ID	55102
Full Name	autophagy related 2B
Synonyms	C14orf103
General protein information	Preferred Names autophagy related 2B Names autophagy-related protein 2 homolog B ATG2 autophagy related 2 homolog B
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q32.2
Summary	This gene encodes a protein required for autophagy. The encoded protein is involved in autophagosome formation. A germline duplication of a region that includes this gene is associated with predisposition to myeloid malignancies. [provided by RefSeq, Jul 2016].
Disorder MIM:	616226

mRNA and Protein(s)

mRNA	Protein	Name
XM_017021397.1	XP_016876886.1	autophagy-related protein 2 homolog B isoform X2
XM_017021397.2	XP_016876886.1	autophagy-related protein 2 homolog B isoform X2
NM_018036.6	NP_060506.5	autophagy-related protein 2 homolog B
XM_006720187.2	XP_006720250.1	autophagy-related protein 2 homolog B isoform X1
NM_018036.7	NP_060506.6	autophagy-related protein 2 homolog B

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Atg2b	NP_083930.5
Canis lupus familiaris (dog)	ATG2B	XP_537549.3
Gallus gallus (chicken)	ATG2B	XP_421350.3
Drosophila melanogaster (fruit fly)	Atg2	NP_001261341.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004092	XP_312964.5
Xenopus tropicalis (tropical clawed frog)	atg2b	NP_001072626.1
Homo sapiens (human)	ATG2B	NP_060506.5
Bos taurus (cattle)	ATG2B	NP_001193682.1
Pan troglodytes (chimpanzee)	ATG2B	XP_510152.3
	ATG2B	XP_001101467.2

Gene Ontology
Bibliography

Related articles in PubMed

Atg2A/B deficiency switches cytoprotective autophagy to non-canonical caspase-8 activation and apoptosis.
Tang Z, Takahashi Y, Chen C, Liu Y, He H, Tsotakos N, Serfass JM, Gebru MT, Chen H, Young MM, Wang HG
Cell death and differentiation24(12)2127-2138(2017 Dec)

Investigation of Association between Autophagy-Related Gene Polymorphisms and Pseudoexfoliation Syndrome and Pseudoexfoliation Glaucoma in a Spanish Population.
de Juan-Marcos L, Escudero-Dom?nguez FA, Hern?ndez-Galilea E, Cruz-Gonz?lez F, Follana-Neira I, Gonz?lez-Sarmiento R
Seminars in ophthalmology1-6(2016 Dec)

Aberrant methylation of ATG2B, ATG4D, ATG9A and ATG9B CpG island promoter is associated with decreased mRNA expression in sporadic breast carcinoma.
Zhang X, Li C, Wang D, Chen Q, Li CL, Li HJ
Gene590(2)285-92(2016 Sep)

Germline duplication of ATG2B and GSKIP predisposes to familial myeloid malignancies.
Saliba J, Saint-Martin C, Di Stefano A, Lenglet G, Marty C, Keren B, Pasquier F, Valle VD, Secardin L, Leroy G, Mahfoudhi E, Grosjean S, Droin N, Diop M, Dessen P, Charrier S, Palazzo A, Merlevede J, Meniane JC, Delaunay-Darivon C, Fuseau P, Isnard F, Casadevall N, Solary E, Debili N, Bernard OA, Raslova H, Najman A, Vainchenker W, Bellann?-Chantelot C, Plo I
Nature genetics47(10)1131-40(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study highlights the transcriptional inactivation mechanisms of ATG2B, ATG4D, ATG9A and ATG9B promoter methylation status and the possible origin of autophagy signal pathway repression in invasive ductal carcinomas.
Title: Aberrant methylation of ATG2B, ATG4D, ATG9A and ATG9B CpG island promoter is associated with decreased mRNA expression in sporadic breast carcinoma.

demonstrate that overexpression of ATG2B and GSKIP enhances hematopoietic progenitor differentiation, including of megakaryocytes, by increasing progenitor sensitivity to thrombopoietin
Title: Germline duplication of ATG2B and GSKIP predisposes to familial myeloid malignancies.

miR-143 inhibits cell proliferation by targeting autophagy-related 2B in non-small cell lung cancer H1299 cells.
Title: miR-143 inhibits cell proliferation by targeting autophagy-related 2B in non-small cell lung cancer H1299 cells.

miR-130a inhibited autophagy by reducing autophagosome formation, an effect mediated by downregulation of the genes ATG2B and DICER1.
Title: miRNA-130a targets ATG2B and DICER1 to inhibit autophagy and trigger killing of chronic lymphocytic leukemia cells.

Depletion of both Atg2A and Atg2B causes clustering of enlarged lipid droplets in an autophagy-independent manner. These data suggest that mammalian Atg2 proteins function both in autophagosome formation and regulation of lipid droplet morphology and dispersion.
Title: Mammalian Atg2 proteins are essential for autophagosome formation and important for regulation of size and distribution of lipid droplets.

The following ATG2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATG2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu107577	NM_018036.7 Latest version!	Homo sapiens autophagy related 2B (ATG2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$779.50-$1091.30 $1559.00
OHu84962	XM_017021397.2 Latest version!	Homo sapiens autophagy related 2B (ATG2B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu02652	NM_018036.6 Latest version!	Homo sapiens autophagy related 2B (ATG2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu47763	XM_006720187.2 Latest version!	Homo sapiens autophagy related 2B (ATG2B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu84962	XM_017021397.1	Homo sapiens autophagy related 2B (ATG2B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATG2B ( NM_018036.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_060506.6 Homo sapiens autophagy related 2B (ATG2B), mRNA.

Price & Availability↑

CloneID	OHu107577
Clone ID Related Accession (Same CDS sequence)	NM_018036.7
Accession Version	NM_018036.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	autophagy-related protein 2 homolog B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL359240.4 and AL355102.5. On Nov 23, 2018 this sequence version replaced NM_018036.6. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.80978.1, SRR1803612.275299.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000359933.6/ ENSP00000353010.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GCCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTTCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAAGGCACCG GGTCCCTCGC CCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCTTG 
GAGTCAGCAG ATGCACCCTT AGAAGTCACT GAAGGATTCA TTCAGTCAAT TTCCCTGTCA 
GTTCCATGGG GCTCTTTACT GCAGGATAAT TGTGCACTGG AAGTGAGAGG ATTAGAAATG 
GTCTTCCGGC CTAGACCTCG CCCAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTT 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CCCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAAAAGTCAC TTTTATAGAT ACTGTTTTGA GAATTGAACA TGTGCCAGAA 
AATTCCAAAA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACTGTGTA CTGTGATGAA 
ACTGCTGACG AATCCTCAGG AATTAATGTG CATCAACCCA CTGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCAGC CAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TCTCACCTAG CTGGAACCCC 
AAAATTATTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGAT AGCACCTTCT 
GACCCAGTGC AGATTGGACG GTTAATTGGT AGGTTGGAGT TGAGTCTCAC GTTGAAACAG 
AATGAAGTGC TTCCTGGAGC TAAGTTGGAT GTTGATGGAC AGATAGACTC TATTCATCTA 
CTCCTGTCAC CAAGACAGGT GCACTTGCTT TTGGATATGT TGGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGGA AAAATCGACC CATGCAGCAG 
GAAGACGAGT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTC 
TCTGTGGGTG TATCTTCAGA GCAAAGCTTT TATGAGACAG AAACAGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTATT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA CCCCCCAAAC ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAATA CCCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC CACTTGGGGA 
GAGTTCCTTG ATCATCATAA AGAACAGCCA GTAAGAGGGT CAACATTTCC ATCCAACCTA 
GTTCACCCAA CACCTTTACA GAAGACATCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCAAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACTT TTTCAATCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAAACGTCA CAGAACCTTA ATCCATTGAC ACCTATGGCA 
GTAGCTTTCT TTACTTGTAT AGAAAAGATT GATCCAGCAA GATTTTCAAC AGAAGATTTT 
AAGTCTTTCC GAGCAGTGTT TGCAGAAGCT TGCTCACACG ATCACCTTAG ATTTATAGGT 
ACTGGCATTA AAGTATCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GATATGTCCA TTGGGCAAAT GGAATTTTTG GAGTGCCTAT TTCCAACTGA TTTTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTAACG TTCCATTCCA AAGAAGAAAC TGGTTCCCAT 
TCCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAGAGGGCC CCAGGGTAAT 
CAAGCAAGAC TTAGTTCAGT TCCTCACAAG GCAGAATTGC AAATTAAATT AAATCCAGTG 
TGTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCACAGAAA 
CTTGCCACAG TAGAGATGAT GGCATCCCAC ATGTATACTT CATATAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTGTTTCTA GATGATTCAC ATAGTCCTGC AAATTGTCGG 
ATATCAGTAC AAGTTGCCAC ACCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGATCTG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATCCTT 
TATTTAGCCT TCACAGATCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAA 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAATTGGAT CGTTCCAGGA AGAGAAAGGA 
GATCCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AACATCGTCA 
GATGACTTTG ACTGGCCACG AATTGTACTG AAAATAAATC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TTGCAGCTGA AGAAGAAGAG GAGAATGATG GTCACTACCA GGAGGAAGAG 
GAAGGAGGTG CTCATTCCTT GAAAGATGTT TGTGATCTAA GAAGACCAGC CCCATCTCCT 
TTTTCTTCTC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACAG AATTTCAGGA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACG 
TTACCAAATA TTTATGTAAC ACTACCTAAT AAGAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTTAATG ACTTGCTACT GTGGGAACCA ACAGCTCCTT CACCAGTGGA GACATTCGAG 
AATATTTCCT ATGGCATTGG GCTTTCAGTA GCCAGTCAGC TCATTAATAC TTTCAACAAA 
GATAGTTTTA GTGCATTTAA ATCTGCAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC CACTGTTGAT CCCAACTATC GTTCTCGCAG GAAAAAAAAA 
TTAGACTCTC AGAACAAGAA CTCTCAGAGT TTTCTCTCAG TTCTTCTGAA TATTAATCAT 
GGATTAATAG CAGTGTTCAC AGATGTGAAG CAAGATAATG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTAGA GTTCAATAGT GGTTCATTAT TTTGTGTGAC AAAATATGAA 
GGTTTTGATG ACAAGCACTA CATTTGCCTT CATTCTAGCA GTTTCAGTCT GTACCATAAA 
GGCATAGTGA ATGGAGTGAT TCTCCCGACA GAAACACGAC TTCCCAGCTC AACCCGCCCA 
CACTGGTTGG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTAA AACTTCTTCA 
GATGGAGTTG GAGGAGACAG TTTGAATATG CTGTCTGTTG CCGTTAAAAT ATTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAATTTCTC ATTGCCGTAG GACTGAAAGG AGCCACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTTT GGGATATAAT CCTCCAACTT CATTTACAAC TTTTCATGTT 
CATCTTTGGA GCTGTGCACT TGATTATAGA CCCCTTTATT TGCCAATCCG ATCTCTTCTT 
ACCGTGGAAA CATTCAGTGT TTCCAGTAGC GTTGCATTGG ATAAATCTTC CTCTACTCTC 
AGAATAATCT TGGATGAAGC TGCTTTACAT CTATCTGACA AATGCAATAC TGTCACTATA 
AATCTGAGTA GAGATTATGT TCGTGTGATG GATATGGGGC TTTTGGAGTT AACCATAACT 
GCAGTGAAGT CTGATTCTGA TGGAGAGCAA ACTGAGCCCC GCTTTGAGTT ACACTGTTCC 
AGCGATGTTG TCCATATCAG AACGTGCTCA GACTCTTGTG CTGCGTTAAT GAATCTCATT 
CAGTACATTG CAAGCTATGG TGACTTGCAG ACACCTAACA AGGCAGATAT GAAGCCTGGA 
GCCTTTCAAA GAAGGTCTAA GGTAGATTCC AGTGGTCGAT CATCCTCACG TGGTCCAGTA 
CTTCCTGAAG CAGATCAACA AATGTTACGA GATCTGATGA GTGATGCTAT GGAGGAGATC 
GACATGCAAC AAGGCACCTC GTCAGTAAAA CCACAGGCTA ATGGTGTTTT GGATGAAAAA 
TCTCAAATTC AGGAGCCATG TTGTTCAGAC CTCTTCCTGT TTCCTGACGA GAGTGGGAAT 
GTATCCCAGG AGTCCGGCCC CACCTATGCC TCATTCTCTC ACCATTTCAT CAGTGATGCA 
ATGACAGGTG TGCCCACTGA GAATGATGAC TTTTGCATTC TTTTTGCACC AAAAGCAGCC 
ATGCAGGAGA AGGAAGAAGA ACCAGTTATA AAAATCATGG TTGATGATGC AATTGTGATA 
AGAGACAATT ATTTCAGTCT GCCCGTTAAT AAGACCGATA CGAGCAAAGC CCCCTTACAC 
TTTCCCATTC CTGTGATTCG CTATGTGGTG AAGGAGGTCT CTCTTGTCTG GCATCTTTAT 
GGAGGAAAGG ATTTTGGAAT AGTCCCTCCC ACTTCTCCGG CTAAAAGTTA TATTAGTCCC 
CACAGTTCGC CTTCTCACAC ACCCACGAGA CATGGACGTA ATACAGTATG TGGGGGAAAA 
GGAAGGAACC ATGACTTTTT AATGGAAATA CAGCTAAGCA AGGTGAAGTT TCAGCATGAA 
GTCTACCCGC CATGCAAACC TGATTGTGAT TCCAGCCTCT CAGAACACCC AGTCTCCCGG 
CAGGTGTTCA TTGTTCAGGA TCTTGAGATT CGAGATCGTT TGGCAACATC ACAAATGAAT 
AAATTTTTAT ACCTGTATTG CAGTAAAGAA ATGCCTCGAA AAGCTCACTC CAACATGTTG 
ACAGTGAAAG CCTTACACGT GTGTCCAGAA TCTGGCAGGT CCCCACAGGA GTGCTGCTTG 
AGAGTGTCGC TGATGCCGCT CCGCCTCAAT ATTGACCAGG ATGCTTTGTT CTTCCTGAAG 
GATTTCTTCA CAAGTCTTTC TGCAGAAGTA GAGCTTCAAA TGACTCCAGA TCCAGAAGTT 
AAAAAGTCTC CTGGAGCTGA TGTCACCTGC AGTTTGCCAA GGCATTTGAG TACCTCAAAG 
GAGCCAAATC TGGTTATTTC TTTCTCTGGG CCAAAACAGC CTTCCCAAAA TGATAGTGCC 
AATTCAGTGG AAGTGGTTAA TGGCATGGAA GAGAAGAACT TCTCTGCTGA AGAAGCATCT 
TTTAGGGATC AGCCTGTGTT TTTTAGAGAA TTTAGATTCA CGTCAGAAGT TCCCATTCGA 
CTTGATTATC ATGGCAAACA TGTATCAATG GATCAGGGTA CGCTAGCTGG GATTTTGATT 
GGTCTGGCTC AGTTAAACTG CTCTGAACTA AAGCTCAAGA GGCTTTCCTA TCGACATGGT 
TTACTAGGCG TTGACAAATT ATTCTCATAT GCAATCACTG AGTGGCTTAA TGACATTAAG 
AAGAACCAGC TACCAGGAAT CCTGGGAGGT GTTGGACCTA TGCATTCACT AGTACAATTA 
GTACAAGGCC TAAAGGACTT GGTCTGGCTC CCAATAGAGC AGTACCGGAA GGATGGCCGC 
ATTGTCAGAG GGTTTCAGAG AGGCGCTGCT TCCTTTGGTA CCTCGACAGC GATGGCTGCT 
CTAGAACTCA CAAACAGAAT GGTTCAAACC ATACAGGCAG CTGCAGAGAC TGCTTATGAT 
ATGGTGTCTC CTGGTACCCT TTCTATCGAG CCCAAGAAGA CCAAAAGGTT TCCTCATCAC 
CGGTTAGCCC ACCAGCCAGT AGACCTGAGG GAAGGTGTGG CCAAGGCCTA CAGTGTTGTG 
AAAGAGGGAA TCACAGACAC GGCTCAGACC ATTTATGAAA CTGCGGCTCG AGAACACGAG 
AGCAGAGGGG TGACTGGTGC CGTGGGCGAG GTTCTGCGCC AGATTCCTCC GGCAGTGGTG 
AAACCTCTGA TTGTTGCCAC AGAAGCAACG TCAAACGTGC TGGGTGGCAT GAGAAACCAA 
ATTAGGCCAG ATGTCCGGCA AGACGAGTCA CAGAAATGGC GCCACGGGGA TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060506.6
CDS	366..6602
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVRGLEMVFRPRPRPATGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTVYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTAPVETEPKLSPSWNPKIIYEPHPQLTRNLPEIAPSDPVQIGRLIGRLELSLTLKQNEVLPGAKLDVDGQIDSIHLLLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSVGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGDPPNMDLELSLTSTYTNTPAGSPLSATVLQPTWGEFLDHHKEQPVRGSTFPSNLVHPTPLQKTSLPSRSVSVDESRPELIFRLAVGTFSISVLHIDPLSPPETSQNLNPLTPMAVAFFTCIEKIDPARFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMEFLECLFPTDFHSVPPHYTELLTFHSKEETGSHSPVCLQLHYKHSENRGPQGNQARLSSVPHKAELQIKLNPVCCELDISIVDRLNSLLQPQKLATVEMMASHMYTSYNKHISLHKAFTEVFLDDSHSPANCRISVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILYLAFTDLEFKTEFIGGSTPEQIKLELTFRELIGSFQEEKGDPSIKFFHVSSGVDGDTTSSDDFDWPRIVLKINPPAMHSILERIAAEEEEENDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQDKAISNSHYVLELTLPNIYVTLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFNKDSFSAFKSAVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLNINHGLIAVFTDVKQDNGDLLENKHGEFWLEFNSGSLFCVTKYEGFDDKHYICLHSSSFSLYHKGIVNGVILPTETRLPSSTRPHWLEPTIYSSEEDGLSKTSSDGVGGDSLNMLSVAVKILSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLSRDYVRVMDMGLLELTITAVKSDSDGEQTEPRFELHCSSDVVHIRTCSDSCAALMNLIQYIASYGDLQTPNKADMKPGAFQRRSKVDSSGRSSSRGPVLPEADQQMLRDLMSDAMEEIDMQQGTSSVKPQANGVLDEKSQIQEPCCSDLFLFPDESGNVSQESGPTYASFSHHFISDAMTGVPTENDDFCILFAPKAAMQEKEEEPVIKIMVDDAIVIRDNYFSLPVNKTDTSKAPLHFPIPVIRYVVKEVSLVWHLYGGKDFGIVPPTSPAKSYISPHSSPSHTPTRHGRNTVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPDCDSSLSEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTVKALHVCPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSAEVELQMTPDPEVKKSPGADVTCSLPRHLSTSKEPNLVISFSGPKQPSQNDSANSVEVVNGMEEKNFSAEEASFRDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLSYRHGLLGVDKLFSYAITEWLNDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKTKRFPHHRLAHQPVDLREGVAKAYSVVKEGITDTAQTIYETAAREHESRGVTGAVGEVLRQIPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGDD

Target ORF information:

RefSeq Version	NM_018036.7
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy related 2B (ATG2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018036.7

ORF Insert Sequence:

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GCCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTTCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAAGGCACCG GGTCCCTCGC CCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCTTG 
GAGTCAGCAG ATGCACCCTT AGAAGTCACT GAAGGATTCA TTCAGTCAAT TTCCCTGTCA 
GTTCCATGGG GCTCTTTACT GCAGGATAAT TGTGCACTGG AAGTGAGAGG ATTAGAAATG 
GTCTTCCGGC CTAGACCTCG CCCAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTT 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CCCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAAAAGTCAC TTTTATAGAT ACTGTTTTGA GAATTGAACA TGTGCCAGAA 
AATTCCAAAA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACTGTGTA CTGTGATGAA 
ACTGCTGACG AATCCTCAGG AATTAATGTG CATCAACCCA CTGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCAGC CAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TCTCACCTAG CTGGAACCCC 
AAAATTATTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGAT AGCACCTTCT 
GACCCAGTGC AGATTGGACG GTTAATTGGT AGGTTGGAGT TGAGTCTCAC GTTGAAACAG 
AATGAAGTGC TTCCTGGAGC TAAGTTGGAT GTTGATGGAC AGATAGACTC TATTCATCTA 
CTCCTGTCAC CAAGACAGGT GCACTTGCTT TTGGATATGT TGGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGGA AAAATCGACC CATGCAGCAG 
GAAGACGAGT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTC 
TCTGTGGGTG TATCTTCAGA GCAAAGCTTT TATGAGACAG AAACAGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTATT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA CCCCCCAAAC ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAATA CCCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC CACTTGGGGA 
GAGTTCCTTG ATCATCATAA AGAACAGCCA GTAAGAGGGT CAACATTTCC ATCCAACCTA 
GTTCACCCAA CACCTTTACA GAAGACATCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCAAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACTT TTTCAATCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAAACGTCA CAGAACCTTA ATCCATTGAC ACCTATGGCA 
GTAGCTTTCT TTACTTGTAT AGAAAAGATT GATCCAGCAA GATTTTCAAC AGAAGATTTT 
AAGTCTTTCC GAGCAGTGTT TGCAGAAGCT TGCTCACACG ATCACCTTAG ATTTATAGGT 
ACTGGCATTA AAGTATCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GATATGTCCA TTGGGCAAAT GGAATTTTTG GAGTGCCTAT TTCCAACTGA TTTTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTAACG TTCCATTCCA AAGAAGAAAC TGGTTCCCAT 
TCCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAGAGGGCC CCAGGGTAAT 
CAAGCAAGAC TTAGTTCAGT TCCTCACAAG GCAGAATTGC AAATTAAATT AAATCCAGTG 
TGTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCACAGAAA 
CTTGCCACAG TAGAGATGAT GGCATCCCAC ATGTATACTT CATATAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTGTTTCTA GATGATTCAC ATAGTCCTGC AAATTGTCGG 
ATATCAGTAC AAGTTGCCAC ACCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGATCTG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATCCTT 
TATTTAGCCT TCACAGATCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAA 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAATTGGAT CGTTCCAGGA AGAGAAAGGA 
GATCCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AACATCGTCA 
GATGACTTTG ACTGGCCACG AATTGTACTG AAAATAAATC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TTGCAGCTGA AGAAGAAGAG GAGAATGATG GTCACTACCA GGAGGAAGAG 
GAAGGAGGTG CTCATTCCTT GAAAGATGTT TGTGATCTAA GAAGACCAGC CCCATCTCCT 
TTTTCTTCTC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACAG AATTTCAGGA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACG 
TTACCAAATA TTTATGTAAC ACTACCTAAT AAGAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTTAATG ACTTGCTACT GTGGGAACCA ACAGCTCCTT CACCAGTGGA GACATTCGAG 
AATATTTCCT ATGGCATTGG GCTTTCAGTA GCCAGTCAGC TCATTAATAC TTTCAACAAA 
GATAGTTTTA GTGCATTTAA ATCTGCAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC CACTGTTGAT CCCAACTATC GTTCTCGCAG GAAAAAAAAA 
TTAGACTCTC AGAACAAGAA CTCTCAGAGT TTTCTCTCAG TTCTTCTGAA TATTAATCAT 
GGATTAATAG CAGTGTTCAC AGATGTGAAG CAAGATAATG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTAGA GTTCAATAGT GGTTCATTAT TTTGTGTGAC AAAATATGAA 
GGTTTTGATG ACAAGCACTA CATTTGCCTT CATTCTAGCA GTTTCAGTCT GTACCATAAA 
GGCATAGTGA ATGGAGTGAT TCTCCCGACA GAAACACGAC TTCCCAGCTC AACCCGCCCA 
CACTGGTTGG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTAA AACTTCTTCA 
GATGGAGTTG GAGGAGACAG TTTGAATATG CTGTCTGTTG CCGTTAAAAT ATTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAATTTCTC ATTGCCGTAG GACTGAAAGG AGCCACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTTT GGGATATAAT CCTCCAACTT CATTTACAAC TTTTCATGTT 
CATCTTTGGA GCTGTGCACT TGATTATAGA CCCCTTTATT TGCCAATCCG ATCTCTTCTT 
ACCGTGGAAA CATTCAGTGT TTCCAGTAGC GTTGCATTGG ATAAATCTTC CTCTACTCTC 
AGAATAATCT TGGATGAAGC TGCTTTACAT CTATCTGACA AATGCAATAC TGTCACTATA 
AATCTGAGTA GAGATTATGT TCGTGTGATG GATATGGGGC TTTTGGAGTT AACCATAACT 
GCAGTGAAGT CTGATTCTGA TGGAGAGCAA ACTGAGCCCC GCTTTGAGTT ACACTGTTCC 
AGCGATGTTG TCCATATCAG AACGTGCTCA GACTCTTGTG CTGCGTTAAT GAATCTCATT 
CAGTACATTG CAAGCTATGG TGACTTGCAG ACACCTAACA AGGCAGATAT GAAGCCTGGA 
GCCTTTCAAA GAAGGTCTAA GGTAGATTCC AGTGGTCGAT CATCCTCACG TGGTCCAGTA 
CTTCCTGAAG CAGATCAACA AATGTTACGA GATCTGATGA GTGATGCTAT GGAGGAGATC 
GACATGCAAC AAGGCACCTC GTCAGTAAAA CCACAGGCTA ATGGTGTTTT GGATGAAAAA 
TCTCAAATTC AGGAGCCATG TTGTTCAGAC CTCTTCCTGT TTCCTGACGA GAGTGGGAAT 
GTATCCCAGG AGTCCGGCCC CACCTATGCC TCATTCTCTC ACCATTTCAT CAGTGATGCA 
ATGACAGGTG TGCCCACTGA GAATGATGAC TTTTGCATTC TTTTTGCACC AAAAGCAGCC 
ATGCAGGAGA AGGAAGAAGA ACCAGTTATA AAAATCATGG TTGATGATGC AATTGTGATA 
AGAGACAATT ATTTCAGTCT GCCCGTTAAT AAGACCGATA CGAGCAAAGC CCCCTTACAC 
TTTCCCATTC CTGTGATTCG CTATGTGGTG AAGGAGGTCT CTCTTGTCTG GCATCTTTAT 
GGAGGAAAGG ATTTTGGAAT AGTCCCTCCC ACTTCTCCGG CTAAAAGTTA TATTAGTCCC 
CACAGTTCGC CTTCTCACAC ACCCACGAGA CATGGACGTA ATACAGTATG TGGGGGAAAA 
GGAAGGAACC ATGACTTTTT AATGGAAATA CAGCTAAGCA AGGTGAAGTT TCAGCATGAA 
GTCTACCCGC CATGCAAACC TGATTGTGAT TCCAGCCTCT CAGAACACCC AGTCTCCCGG 
CAGGTGTTCA TTGTTCAGGA TCTTGAGATT CGAGATCGTT TGGCAACATC ACAAATGAAT 
AAATTTTTAT ACCTGTATTG CAGTAAAGAA ATGCCTCGAA AAGCTCACTC CAACATGTTG 
ACAGTGAAAG CCTTACACGT GTGTCCAGAA TCTGGCAGGT CCCCACAGGA GTGCTGCTTG 
AGAGTGTCGC TGATGCCGCT CCGCCTCAAT ATTGACCAGG ATGCTTTGTT CTTCCTGAAG 
GATTTCTTCA CAAGTCTTTC TGCAGAAGTA GAGCTTCAAA TGACTCCAGA TCCAGAAGTT 
AAAAAGTCTC CTGGAGCTGA TGTCACCTGC AGTTTGCCAA GGCATTTGAG TACCTCAAAG 
GAGCCAAATC TGGTTATTTC TTTCTCTGGG CCAAAACAGC CTTCCCAAAA TGATAGTGCC 
AATTCAGTGG AAGTGGTTAA TGGCATGGAA GAGAAGAACT TCTCTGCTGA AGAAGCATCT 
TTTAGGGATC AGCCTGTGTT TTTTAGAGAA TTTAGATTCA CGTCAGAAGT TCCCATTCGA 
CTTGATTATC ATGGCAAACA TGTATCAATG GATCAGGGTA CGCTAGCTGG GATTTTGATT 
GGTCTGGCTC AGTTAAACTG CTCTGAACTA AAGCTCAAGA GGCTTTCCTA TCGACATGGT 
TTACTAGGCG TTGACAAATT ATTCTCATAT GCAATCACTG AGTGGCTTAA TGACATTAAG 
AAGAACCAGC TACCAGGAAT CCTGGGAGGT GTTGGACCTA TGCATTCACT AGTACAATTA 
GTACAAGGCC TAAAGGACTT GGTCTGGCTC CCAATAGAGC AGTACCGGAA GGATGGCCGC 
ATTGTCAGAG GGTTTCAGAG AGGCGCTGCT TCCTTTGGTA CCTCGACAGC GATGGCTGCT 
CTAGAACTCA CAAACAGAAT GGTTCAAACC ATACAGGCAG CTGCAGAGAC TGCTTATGAT 
ATGGTGTCTC CTGGTACCCT TTCTATCGAG CCCAAGAAGA CCAAAAGGTT TCCTCATCAC 
CGGTTAGCCC ACCAGCCAGT AGACCTGAGG GAAGGTGTGG CCAAGGCCTA CAGTGTTGTG 
AAAGAGGGAA TCACAGACAC GGCTCAGACC ATTTATGAAA CTGCGGCTCG AGAACACGAG 
AGCAGAGGGG TGACTGGTGC CGTGGGCGAG GTTCTGCGCC AGATTCCTCC GGCAGTGGTG 
AAACCTCTGA TTGTTGCCAC AGAAGCAACG TCAAACGTGC TGGGTGGCAT GAGAAACCAA 
ATTAGGCCAG ATGTCCGGCA AGACGAGTCA CAGAAATGGC GCCACGGGGA TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_017021397.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876886.1 Homo sapiens autophagy related 2B (ATG2B), transcript variant X3, mRNA.

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CloneID	OHu84962
Clone ID Related Accession (Same CDS sequence)	XM_017021397.1 , XM_017021397.2
Accession Version	XM_017021397.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	autophagy-related protein 2 homolog B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GCCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTTCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAAGGCACCG GGTCCCTCGC CCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCTTG 
GAGTCAGCAG ATGCACCCTT AGAAGTCACT GAAGGATTCA TTCAGTCAAT TTCCCTGTCA 
GTTCCATGGG GCTCTTTACT GCAGGATAAT TGTGCACTGG AAGTGAGAGG ATTAGAAATG 
GTCTTCCGGC CTAGACCTCG CCCAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTT 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CCCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAAAAGTCAC TTTTATAGAT ACTGTTTTGA GAATTGAACA TGTGCCAGAA 
AATTCCAAAA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACTGTGTA CTGTGATGAA 
ACTGCTGACG AATCCTCAGG AATTAATGTG CATCAACCCA CTGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCAGC CAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TCTCACCTAG CTGGAACCCC 
AAAATTATTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGAT AGCACCTTCT 
GACCCAGTGC AGATTGGACG GTTAATTGGT AGGTTGGAGT TGAGTCTCAC GTTGAAACAG 
AATGAAGTGC TTCCTGGAGC TAAGTTGGAT GTTGATGGAC AGATAGACTC TATTCATCTA 
CTCCTGTCAC CAAGACAGGT GCACTTGCTT TTGGATATGT TGGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGGA AAAATCGACC CATGCAGCAG 
GAAGACGAGT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTC 
TCTGTGGGTG TATCTTCAGA GCAAAGCTTT TATGAGACAG AAACAGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTATT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA CCCCCCAAAC ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAATA CCCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC CACTTGGGGA 
GAGTTCCTTG ATCATCATAA AGAACAGCCA GTAAGAGGGT CAACATTTCC ATCCAACCTA 
GTTCACCCAA CACCTTTACA GAAGACATCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCAAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACTT TTTCAATCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAAACGTCA CAGAACCTTA ATCCATTGAC ACCTATGGCA 
GTAGCTTTCT TTACTTGTAT AGAAAAGATT GATCCAGCAA GATTTTCAAC AGAAGATTTT 
AAGTCTTTCC GAGCAGTGTT TGCAGAAGCT TGCTCACACG ATCACCTTAG ATTTATAGGT 
ACTGGCATTA AAGTATCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GATATGTCCA TTGGGCAAAT GGAATTTTTG GAGTGCCTAT TTCCAACTGA TTTTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTAACG TTCCATTCCA AAGAAGAAAC TGGTTCCCAT 
TCCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAGAGGGCC CCAGGGTAAT 
CAAGCAAGAC TTAGTTCAGT TCCTCACAAG GCAGAATTGC AAATTAAATT AAATCCAGTG 
TGTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCACAGAAA 
CTTGCCACAG TAGAGATGAT GGCATCCCAC ATGTATACTT CATATAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTGTTTCTA GATGATTCAC ATAGTCCTGC AAATTGTCGG 
ATATCAGTAC AAGTTGCCAC ACCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGATCTG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATCCTT 
TATTTAGCCT TCACAGATCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAA 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAATTGGAT CGTTCCAGGA AGAGAAAGGA 
GATCCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AACATCGTCA 
GATGACTTTG ACTGGCCACG AATTGTACTG AAAATAAATC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TTGCAGCTGA AGAAGAAGAG GAGAATGATG GTCACTACCA GGAGGAAGAG 
GAAGGAGGTG CTCATTCCTT GAAAGATGTT TGTGATCTAA GAAGACCAGC CCCATCTCCT 
TTTTCTTCTC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACAG AATTTCAGGA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACG 
TTACCAAATA TTTATGTAAC ACTACCTAAT AAGAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTTAATG ACTTGCTACT GTGGGAACCA ACAGCTCCTT CACCAGTGGA GACATTCGAG 
AATATTTCCT ATGGCATTGG GCTTTCAGTA GCCAGTCAGC TCATTAATAC TTTCAACAAA 
GATAGTTTTA GTGCATTTAA ATCTGCAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC CACTGTTGAT CCCAACTATC GTTCTCGCAG GAAAAAAAAA 
TTAGACTCTC AGAACAAGAA CTCTCAGAGT TTTCTCTCAG TTCTTCTGAA TATTAATCAT 
GGATTAATAG CAGTGTTCAC AGATGTGAAG CAAGATAATG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTAGA GTTCAATAGT GGTTCATTAT TTTGTGTGAC AAAATATGAA 
GGTTTTGATG ACAAGCACTA CATTTGCCTT CATTCTAGCA GTTTCAGTCT GTACCATAAA 
GGCATAGTGA ATGGAGTGAT TCTCCCGACA GAAACACGAC TTCCCAGCTC AACCCGCCCA 
CACTGGTTGG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTAA AACTTCTTCA 
GATGGAGTTG GAGGAGACAG TTTGAATATG CTGTCTGTTG CCGTTAAAAT ATTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAATTTCTC ATTGCCGTAG GACTGAAAGG AGCCACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTTT GGGATATAAT CCTCCAACTT CATTTACAAC TTTTCATGTT 
CATCTTTGGA GCTGTGCACT TGATTATAGA CCCCTTTATT TGCCAATCCG ATCTCTTCTT 
ACCGTGGAAA CATTCAGTGT TTCCAGTAGC GTTGCATTGG ATAAATCTTC CTCTACTCTC 
AGAATAATCT TGGATGAAGC TGCTTTACAT CTATCTGACA AATGCAATAC TGTCACTATA 
AATCTGAGTA GAGATTATGT TCGTGTGATG GATATGGGGC TTTTGGAGTT AACCATAACT 
GCAGTGAAGT CTGATTCTGA TGGAGAGCAA ACTGAGCCCC GCTTTGAGTT ACACTGTTCC 
AGCGATGTTG TCCATATCAG AACGTGCTCA GACTCTTGTG CTGCGTTAAT GAATCTCATT 
CAGTACATTG CAAGCTATGG TGACTTGCAG ACACCTAACA AGGCAGATAT GAAGCCTGGA 
GCCTTTCAAA GAAGGTCTAA GGTAGATTCC AGTGGTCGAT CATCCTCACG TGGTCCAGTA 
CTTCCTGAAG CAGATCAACA AATGTTACGA GATCTGATGA GTGATGCTAT GGAGGAGATC 
GACATGCAAC AAGGCACCTC GTCAGTAAAA CCACAGGCTA ATGGTGTTTT GGATGAAAAA 
TCTCAAATTC AGGAGCCATG TTGTTCAGAC CTCTTCCTGT TTCCTGACGA GAGTGGGAAT 
GTATCCCAGG AGTCCGGCCC CACCTATGCC TCATTCTCTC ACCATTTCAT CAGTGATGCA 
ATGACAGGTG TGCCCACTGA GAATGATGAC TTTTGCATTC TTTTTGCACC AAAAGCAGCC 
ATGCAGGAGA AGGAAGAAGA ACCAGTTATA AAAATCATGG TTGATGATGC AATTGTGATA 
AGAGACAATT ATTTCAGTCT GCCCGTTAAT AAGACCGATA CGAGCAAAGC CCCCTTACAC 
TTTCCCATTC CTGTGATTCG CTATGTGGTG AAGGAGGTCT CTCTTGTCTG GCATCTTTAT 
GGAGGAAAGG ATTTTGGAAT AGTCCCTCCC ACTTCTCCGG CTAAAAGTTA TATTAGTCCC 
CACAGTTCGC CTTCTCACAC ACCCACGAGA CATGGACGTA ATACAGTATG TGGGGGAAAA 
GGAAGGAACC ATGACTTTTT AATGGAAATA CAGCTAAGCA AGGTGAAGTT TCAGCATGAA 
GTCTACCCGC CATGCAAACC TGATTGTGAT TCCAGCCTCT CAGAACACCC AGTCTCCCGG 
CAGGTGTTCA TTGTTCAGGA TCTTGAGATT CGAGATCGTT TGGCAACATC ACAAATGAAT 
AAATTTTTAT ACCTGTATTG CAGTAAAGAA ATGCCTCGAA AAGCTCACTC CAACATGTTG 
ACAGTGAAAG CCTTACACGT GTGTCCAGAA TCTGGCAGGT CCCCACAGGA GTGCTGCTTG 
AGAGTGTCGC TGATGCCGCT CCGCCTCAAT ATTGACCAGG ATGCTTTGTT CTTCCTGAAG 
GATTTCTTCA CAAGTCTTTC TGCAGAAGTA GAGCTTCAAA TGACTCCAGA TCCAGAAGTT 
AAAAAGTCTC CTGGAGCTGA TGTCACCTGC AGTTTGCCAA GGCATTTGAG TACCTCAAAG 
GAGCCAAATC TGGTTATTTC TTTCTCTGGG CCAAAACAGC CTTCCCAAAA TGATAGTGCC 
AATTCAGTGG AAGTGGTTAA TGGCATGGAA GAGAAGAACT TCTCTGCTGA AGAAGCATCT 
TTTAGGGATC AGCCTGTGTT TTTTAGAGAA TTTAGATTCA CGTCAGAAGT TCCCATTCGA 
CTTGATTATC ATGGCAAACA TGTATCAATG GATCAGGGTA CGCTAGCTGG GATTTTGATT 
GGTCTGGCTC AGTTAAACTG CTCTGAACTA AAGCTCAAGA GGCTTTCCTA TCGACATGGT 
TTACTAGGCG TTGACAAATT ATTCTCATAT GCAATCACTG AGTGGCTTAA TGACATTAAG 
AAGAACCAGC TACCAGGAAT CCTGGGAGGT GTTGGACCTA TGCATTCACT AGTACAATTA 
GTACAAGGCC TAAAGGACTT GGTCTGGCTC CCAATAGAGC AGTACCGGAA GGATGGCCGC 
ATTGTCAGAG GGTTTCAGAG AGGCGCTGCT TCCTTTGGTA CCTCGACAGC GATGGCTGCT 
CTAGAACTCA CAAACAGAAT GGTTCAAACC ATACAGGTAA ATTGTAAACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876886.1
CDS	366..6239
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVRGLEMVFRPRPRPATGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTVYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTAPVETEPKLSPSWNPKIIYEPHPQLTRNLPEIAPSDPVQIGRLIGRLELSLTLKQNEVLPGAKLDVDGQIDSIHLLLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSVGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGDPPNMDLELSLTSTYTNTPAGSPLSATVLQPTWGEFLDHHKEQPVRGSTFPSNLVHPTPLQKTSLPSRSVSVDESRPELIFRLAVGTFSISVLHIDPLSPPETSQNLNPLTPMAVAFFTCIEKIDPARFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMEFLECLFPTDFHSVPPHYTELLTFHSKEETGSHSPVCLQLHYKHSENRGPQGNQARLSSVPHKAELQIKLNPVCCELDISIVDRLNSLLQPQKLATVEMMASHMYTSYNKHISLHKAFTEVFLDDSHSPANCRISVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILYLAFTDLEFKTEFIGGSTPEQIKLELTFRELIGSFQEEKGDPSIKFFHVSSGVDGDTTSSDDFDWPRIVLKINPPAMHSILERIAAEEEEENDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQDKAISNSHYVLELTLPNIYVTLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFNKDSFSAFKSAVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLNINHGLIAVFTDVKQDNGDLLENKHGEFWLEFNSGSLFCVTKYEGFDDKHYICLHSSSFSLYHKGIVNGVILPTETRLPSSTRPHWLEPTIYSSEEDGLSKTSSDGVGGDSLNMLSVAVKILSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLSRDYVRVMDMGLLELTITAVKSDSDGEQTEPRFELHCSSDVVHIRTCSDSCAALMNLIQYIASYGDLQTPNKADMKPGAFQRRSKVDSSGRSSSRGPVLPEADQQMLRDLMSDAMEEIDMQQGTSSVKPQANGVLDEKSQIQEPCCSDLFLFPDESGNVSQESGPTYASFSHHFISDAMTGVPTENDDFCILFAPKAAMQEKEEEPVIKIMVDDAIVIRDNYFSLPVNKTDTSKAPLHFPIPVIRYVVKEVSLVWHLYGGKDFGIVPPTSPAKSYISPHSSPSHTPTRHGRNTVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPDCDSSLSEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTVKALHVCPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSAEVELQMTPDPEVKKSPGADVTCSLPRHLSTSKEPNLVISFSGPKQPSQNDSANSVEVVNGMEEKNFSAEEASFRDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLSYRHGLLGVDKLFSYAITEWLNDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQVNCKH

Target ORF information:

RefSeq Version	XM_017021397.1
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy related 2B (ATG2B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021397.1

ORF Insert Sequence:

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GCCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTTCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAAGGCACCG GGTCCCTCGC CCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCTTG 
GAGTCAGCAG ATGCACCCTT AGAAGTCACT GAAGGATTCA TTCAGTCAAT TTCCCTGTCA 
GTTCCATGGG GCTCTTTACT GCAGGATAAT TGTGCACTGG AAGTGAGAGG ATTAGAAATG 
GTCTTCCGGC CTAGACCTCG CCCAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTT 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CCCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAAAAGTCAC TTTTATAGAT ACTGTTTTGA GAATTGAACA TGTGCCAGAA 
AATTCCAAAA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACTGTGTA CTGTGATGAA 
ACTGCTGACG AATCCTCAGG AATTAATGTG CATCAACCCA CTGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCAGC CAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TCTCACCTAG CTGGAACCCC 
AAAATTATTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGAT AGCACCTTCT 
GACCCAGTGC AGATTGGACG GTTAATTGGT AGGTTGGAGT TGAGTCTCAC GTTGAAACAG 
AATGAAGTGC TTCCTGGAGC TAAGTTGGAT GTTGATGGAC AGATAGACTC TATTCATCTA 
CTCCTGTCAC CAAGACAGGT GCACTTGCTT TTGGATATGT TGGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGGA AAAATCGACC CATGCAGCAG 
GAAGACGAGT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTC 
TCTGTGGGTG TATCTTCAGA GCAAAGCTTT TATGAGACAG AAACAGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTATT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA CCCCCCAAAC ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAATA CCCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC CACTTGGGGA 
GAGTTCCTTG ATCATCATAA AGAACAGCCA GTAAGAGGGT CAACATTTCC ATCCAACCTA 
GTTCACCCAA CACCTTTACA GAAGACATCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCAAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACTT TTTCAATCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAAACGTCA CAGAACCTTA ATCCATTGAC ACCTATGGCA 
GTAGCTTTCT TTACTTGTAT AGAAAAGATT GATCCAGCAA GATTTTCAAC AGAAGATTTT 
AAGTCTTTCC GAGCAGTGTT TGCAGAAGCT TGCTCACACG ATCACCTTAG ATTTATAGGT 
ACTGGCATTA AAGTATCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GATATGTCCA TTGGGCAAAT GGAATTTTTG GAGTGCCTAT TTCCAACTGA TTTTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTAACG TTCCATTCCA AAGAAGAAAC TGGTTCCCAT 
TCCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAGAGGGCC CCAGGGTAAT 
CAAGCAAGAC TTAGTTCAGT TCCTCACAAG GCAGAATTGC AAATTAAATT AAATCCAGTG 
TGTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCACAGAAA 
CTTGCCACAG TAGAGATGAT GGCATCCCAC ATGTATACTT CATATAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTGTTTCTA GATGATTCAC ATAGTCCTGC AAATTGTCGG 
ATATCAGTAC AAGTTGCCAC ACCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGATCTG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATCCTT 
TATTTAGCCT TCACAGATCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAA 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAATTGGAT CGTTCCAGGA AGAGAAAGGA 
GATCCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AACATCGTCA 
GATGACTTTG ACTGGCCACG AATTGTACTG AAAATAAATC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TTGCAGCTGA AGAAGAAGAG GAGAATGATG GTCACTACCA GGAGGAAGAG 
GAAGGAGGTG CTCATTCCTT GAAAGATGTT TGTGATCTAA GAAGACCAGC CCCATCTCCT 
TTTTCTTCTC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACAG AATTTCAGGA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACG 
TTACCAAATA TTTATGTAAC ACTACCTAAT AAGAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTTAATG ACTTGCTACT GTGGGAACCA ACAGCTCCTT CACCAGTGGA GACATTCGAG 
AATATTTCCT ATGGCATTGG GCTTTCAGTA GCCAGTCAGC TCATTAATAC TTTCAACAAA 
GATAGTTTTA GTGCATTTAA ATCTGCAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC CACTGTTGAT CCCAACTATC GTTCTCGCAG GAAAAAAAAA 
TTAGACTCTC AGAACAAGAA CTCTCAGAGT TTTCTCTCAG TTCTTCTGAA TATTAATCAT 
GGATTAATAG CAGTGTTCAC AGATGTGAAG CAAGATAATG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTAGA GTTCAATAGT GGTTCATTAT TTTGTGTGAC AAAATATGAA 
GGTTTTGATG ACAAGCACTA CATTTGCCTT CATTCTAGCA GTTTCAGTCT GTACCATAAA 
GGCATAGTGA ATGGAGTGAT TCTCCCGACA GAAACACGAC TTCCCAGCTC AACCCGCCCA 
CACTGGTTGG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTAA AACTTCTTCA 
GATGGAGTTG GAGGAGACAG TTTGAATATG CTGTCTGTTG CCGTTAAAAT ATTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAATTTCTC ATTGCCGTAG GACTGAAAGG AGCCACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTTT GGGATATAAT CCTCCAACTT CATTTACAAC TTTTCATGTT 
CATCTTTGGA GCTGTGCACT TGATTATAGA CCCCTTTATT TGCCAATCCG ATCTCTTCTT 
ACCGTGGAAA CATTCAGTGT TTCCAGTAGC GTTGCATTGG ATAAATCTTC CTCTACTCTC 
AGAATAATCT TGGATGAAGC TGCTTTACAT CTATCTGACA AATGCAATAC TGTCACTATA 
AATCTGAGTA GAGATTATGT TCGTGTGATG GATATGGGGC TTTTGGAGTT AACCATAACT 
GCAGTGAAGT CTGATTCTGA TGGAGAGCAA ACTGAGCCCC GCTTTGAGTT ACACTGTTCC 
AGCGATGTTG TCCATATCAG AACGTGCTCA GACTCTTGTG CTGCGTTAAT GAATCTCATT 
CAGTACATTG CAAGCTATGG TGACTTGCAG ACACCTAACA AGGCAGATAT GAAGCCTGGA 
GCCTTTCAAA GAAGGTCTAA GGTAGATTCC AGTGGTCGAT CATCCTCACG TGGTCCAGTA 
CTTCCTGAAG CAGATCAACA AATGTTACGA GATCTGATGA GTGATGCTAT GGAGGAGATC 
GACATGCAAC AAGGCACCTC GTCAGTAAAA CCACAGGCTA ATGGTGTTTT GGATGAAAAA 
TCTCAAATTC AGGAGCCATG TTGTTCAGAC CTCTTCCTGT TTCCTGACGA GAGTGGGAAT 
GTATCCCAGG AGTCCGGCCC CACCTATGCC TCATTCTCTC ACCATTTCAT CAGTGATGCA 
ATGACAGGTG TGCCCACTGA GAATGATGAC TTTTGCATTC TTTTTGCACC AAAAGCAGCC 
ATGCAGGAGA AGGAAGAAGA ACCAGTTATA AAAATCATGG TTGATGATGC AATTGTGATA 
AGAGACAATT ATTTCAGTCT GCCCGTTAAT AAGACCGATA CGAGCAAAGC CCCCTTACAC 
TTTCCCATTC CTGTGATTCG CTATGTGGTG AAGGAGGTCT CTCTTGTCTG GCATCTTTAT 
GGAGGAAAGG ATTTTGGAAT AGTCCCTCCC ACTTCTCCGG CTAAAAGTTA TATTAGTCCC 
CACAGTTCGC CTTCTCACAC ACCCACGAGA CATGGACGTA ATACAGTATG TGGGGGAAAA 
GGAAGGAACC ATGACTTTTT AATGGAAATA CAGCTAAGCA AGGTGAAGTT TCAGCATGAA 
GTCTACCCGC CATGCAAACC TGATTGTGAT TCCAGCCTCT CAGAACACCC AGTCTCCCGG 
CAGGTGTTCA TTGTTCAGGA TCTTGAGATT CGAGATCGTT TGGCAACATC ACAAATGAAT 
AAATTTTTAT ACCTGTATTG CAGTAAAGAA ATGCCTCGAA AAGCTCACTC CAACATGTTG 
ACAGTGAAAG CCTTACACGT GTGTCCAGAA TCTGGCAGGT CCCCACAGGA GTGCTGCTTG 
AGAGTGTCGC TGATGCCGCT CCGCCTCAAT ATTGACCAGG ATGCTTTGTT CTTCCTGAAG 
GATTTCTTCA CAAGTCTTTC TGCAGAAGTA GAGCTTCAAA TGACTCCAGA TCCAGAAGTT 
AAAAAGTCTC CTGGAGCTGA TGTCACCTGC AGTTTGCCAA GGCATTTGAG TACCTCAAAG 
GAGCCAAATC TGGTTATTTC TTTCTCTGGG CCAAAACAGC CTTCCCAAAA TGATAGTGCC 
AATTCAGTGG AAGTGGTTAA TGGCATGGAA GAGAAGAACT TCTCTGCTGA AGAAGCATCT 
TTTAGGGATC AGCCTGTGTT TTTTAGAGAA TTTAGATTCA CGTCAGAAGT TCCCATTCGA 
CTTGATTATC ATGGCAAACA TGTATCAATG GATCAGGGTA CGCTAGCTGG GATTTTGATT 
GGTCTGGCTC AGTTAAACTG CTCTGAACTA AAGCTCAAGA GGCTTTCCTA TCGACATGGT 
TTACTAGGCG TTGACAAATT ATTCTCATAT GCAATCACTG AGTGGCTTAA TGACATTAAG 
AAGAACCAGC TACCAGGAAT CCTGGGAGGT GTTGGACCTA TGCATTCACT AGTACAATTA 
GTACAAGGCC TAAAGGACTT GGTCTGGCTC CCAATAGAGC AGTACCGGAA GGATGGCCGC 
ATTGTCAGAG GGTTTCAGAG AGGCGCTGCT TCCTTTGGTA CCTCGACAGC GATGGCTGCT 
CTAGAACTCA CAAACAGAAT GGTTCAAACC ATACAGGTAA ATTGTAAACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_017021397.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876886.1 Homo sapiens autophagy related 2B (ATG2B), transcript variant X3, mRNA.

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CloneID	OHu84962
Clone ID Related Accession (Same CDS sequence)	XM_017021397.1 , XM_017021397.2
Accession Version	XM_017021397.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	autophagy-related protein 2 homolog B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017021397.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GCCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTTCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAAGGCACCG GGTCCCTCGC CCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCTTG 
GAGTCAGCAG ATGCACCCTT AGAAGTCACT GAAGGATTCA TTCAGTCAAT TTCCCTGTCA 
GTTCCATGGG GCTCTTTACT GCAGGATAAT TGTGCACTGG AAGTGAGAGG ATTAGAAATG 
GTCTTCCGGC CTAGACCTCG CCCAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTT 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CCCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAAAAGTCAC TTTTATAGAT ACTGTTTTGA GAATTGAACA TGTGCCAGAA 
AATTCCAAAA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACTGTGTA CTGTGATGAA 
ACTGCTGACG AATCCTCAGG AATTAATGTG CATCAACCCA CTGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCAGC CAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TCTCACCTAG CTGGAACCCC 
AAAATTATTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGAT AGCACCTTCT 
GACCCAGTGC AGATTGGACG GTTAATTGGT AGGTTGGAGT TGAGTCTCAC GTTGAAACAG 
AATGAAGTGC TTCCTGGAGC TAAGTTGGAT GTTGATGGAC AGATAGACTC TATTCATCTA 
CTCCTGTCAC CAAGACAGGT GCACTTGCTT TTGGATATGT TGGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGGA AAAATCGACC CATGCAGCAG 
GAAGACGAGT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTC 
TCTGTGGGTG TATCTTCAGA GCAAAGCTTT TATGAGACAG AAACAGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTATT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA CCCCCCAAAC ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAATA CCCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC CACTTGGGGA 
GAGTTCCTTG ATCATCATAA AGAACAGCCA GTAAGAGGGT CAACATTTCC ATCCAACCTA 
GTTCACCCAA CACCTTTACA GAAGACATCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCAAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACTT TTTCAATCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAAACGTCA CAGAACCTTA ATCCATTGAC ACCTATGGCA 
GTAGCTTTCT TTACTTGTAT AGAAAAGATT GATCCAGCAA GATTTTCAAC AGAAGATTTT 
AAGTCTTTCC GAGCAGTGTT TGCAGAAGCT TGCTCACACG ATCACCTTAG ATTTATAGGT 
ACTGGCATTA AAGTATCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GATATGTCCA TTGGGCAAAT GGAATTTTTG GAGTGCCTAT TTCCAACTGA TTTTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTAACG TTCCATTCCA AAGAAGAAAC TGGTTCCCAT 
TCCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAGAGGGCC CCAGGGTAAT 
CAAGCAAGAC TTAGTTCAGT TCCTCACAAG GCAGAATTGC AAATTAAATT AAATCCAGTG 
TGTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCACAGAAA 
CTTGCCACAG TAGAGATGAT GGCATCCCAC ATGTATACTT CATATAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTGTTTCTA GATGATTCAC ATAGTCCTGC AAATTGTCGG 
ATATCAGTAC AAGTTGCCAC ACCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGATCTG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATCCTT 
TATTTAGCCT TCACAGATCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAA 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAATTGGAT CGTTCCAGGA AGAGAAAGGA 
GATCCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AACATCGTCA 
GATGACTTTG ACTGGCCACG AATTGTACTG AAAATAAATC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TTGCAGCTGA AGAAGAAGAG GAGAATGATG GTCACTACCA GGAGGAAGAG 
GAAGGAGGTG CTCATTCCTT GAAAGATGTT TGTGATCTAA GAAGACCAGC CCCATCTCCT 
TTTTCTTCTC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACAG AATTTCAGGA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACG 
TTACCAAATA TTTATGTAAC ACTACCTAAT AAGAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTTAATG ACTTGCTACT GTGGGAACCA ACAGCTCCTT CACCAGTGGA GACATTCGAG 
AATATTTCCT ATGGCATTGG GCTTTCAGTA GCCAGTCAGC TCATTAATAC TTTCAACAAA 
GATAGTTTTA GTGCATTTAA ATCTGCAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC CACTGTTGAT CCCAACTATC GTTCTCGCAG GAAAAAAAAA 
TTAGACTCTC AGAACAAGAA CTCTCAGAGT TTTCTCTCAG TTCTTCTGAA TATTAATCAT 
GGATTAATAG CAGTGTTCAC AGATGTGAAG CAAGATAATG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTAGA GTTCAATAGT GGTTCATTAT TTTGTGTGAC AAAATATGAA 
GGTTTTGATG ACAAGCACTA CATTTGCCTT CATTCTAGCA GTTTCAGTCT GTACCATAAA 
GGCATAGTGA ATGGAGTGAT TCTCCCGACA GAAACACGAC TTCCCAGCTC AACCCGCCCA 
CACTGGTTGG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTAA AACTTCTTCA 
GATGGAGTTG GAGGAGACAG TTTGAATATG CTGTCTGTTG CCGTTAAAAT ATTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAATTTCTC ATTGCCGTAG GACTGAAAGG AGCCACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTTT GGGATATAAT CCTCCAACTT CATTTACAAC TTTTCATGTT 
CATCTTTGGA GCTGTGCACT TGATTATAGA CCCCTTTATT TGCCAATCCG ATCTCTTCTT 
ACCGTGGAAA CATTCAGTGT TTCCAGTAGC GTTGCATTGG ATAAATCTTC CTCTACTCTC 
AGAATAATCT TGGATGAAGC TGCTTTACAT CTATCTGACA AATGCAATAC TGTCACTATA 
AATCTGAGTA GAGATTATGT TCGTGTGATG GATATGGGGC TTTTGGAGTT AACCATAACT 
GCAGTGAAGT CTGATTCTGA TGGAGAGCAA ACTGAGCCCC GCTTTGAGTT ACACTGTTCC 
AGCGATGTTG TCCATATCAG AACGTGCTCA GACTCTTGTG CTGCGTTAAT GAATCTCATT 
CAGTACATTG CAAGCTATGG TGACTTGCAG ACACCTAACA AGGCAGATAT GAAGCCTGGA 
GCCTTTCAAA GAAGGTCTAA GGTAGATTCC AGTGGTCGAT CATCCTCACG TGGTCCAGTA 
CTTCCTGAAG CAGATCAACA AATGTTACGA GATCTGATGA GTGATGCTAT GGAGGAGATC 
GACATGCAAC AAGGCACCTC GTCAGTAAAA CCACAGGCTA ATGGTGTTTT GGATGAAAAA 
TCTCAAATTC AGGAGCCATG TTGTTCAGAC CTCTTCCTGT TTCCTGACGA GAGTGGGAAT 
GTATCCCAGG AGTCCGGCCC CACCTATGCC TCATTCTCTC ACCATTTCAT CAGTGATGCA 
ATGACAGGTG TGCCCACTGA GAATGATGAC TTTTGCATTC TTTTTGCACC AAAAGCAGCC 
ATGCAGGAGA AGGAAGAAGA ACCAGTTATA AAAATCATGG TTGATGATGC AATTGTGATA 
AGAGACAATT ATTTCAGTCT GCCCGTTAAT AAGACCGATA CGAGCAAAGC CCCCTTACAC 
TTTCCCATTC CTGTGATTCG CTATGTGGTG AAGGAGGTCT CTCTTGTCTG GCATCTTTAT 
GGAGGAAAGG ATTTTGGAAT AGTCCCTCCC ACTTCTCCGG CTAAAAGTTA TATTAGTCCC 
CACAGTTCGC CTTCTCACAC ACCCACGAGA CATGGACGTA ATACAGTATG TGGGGGAAAA 
GGAAGGAACC ATGACTTTTT AATGGAAATA CAGCTAAGCA AGGTGAAGTT TCAGCATGAA 
GTCTACCCGC CATGCAAACC TGATTGTGAT TCCAGCCTCT CAGAACACCC AGTCTCCCGG 
CAGGTGTTCA TTGTTCAGGA TCTTGAGATT CGAGATCGTT TGGCAACATC ACAAATGAAT 
AAATTTTTAT ACCTGTATTG CAGTAAAGAA ATGCCTCGAA AAGCTCACTC CAACATGTTG 
ACAGTGAAAG CCTTACACGT GTGTCCAGAA TCTGGCAGGT CCCCACAGGA GTGCTGCTTG 
AGAGTGTCGC TGATGCCGCT CCGCCTCAAT ATTGACCAGG ATGCTTTGTT CTTCCTGAAG 
GATTTCTTCA CAAGTCTTTC TGCAGAAGTA GAGCTTCAAA TGACTCCAGA TCCAGAAGTT 
AAAAAGTCTC CTGGAGCTGA TGTCACCTGC AGTTTGCCAA GGCATTTGAG TACCTCAAAG 
GAGCCAAATC TGGTTATTTC TTTCTCTGGG CCAAAACAGC CTTCCCAAAA TGATAGTGCC 
AATTCAGTGG AAGTGGTTAA TGGCATGGAA GAGAAGAACT TCTCTGCTGA AGAAGCATCT 
TTTAGGGATC AGCCTGTGTT TTTTAGAGAA TTTAGATTCA CGTCAGAAGT TCCCATTCGA 
CTTGATTATC ATGGCAAACA TGTATCAATG GATCAGGGTA CGCTAGCTGG GATTTTGATT 
GGTCTGGCTC AGTTAAACTG CTCTGAACTA AAGCTCAAGA GGCTTTCCTA TCGACATGGT 
TTACTAGGCG TTGACAAATT ATTCTCATAT GCAATCACTG AGTGGCTTAA TGACATTAAG 
AAGAACCAGC TACCAGGAAT CCTGGGAGGT GTTGGACCTA TGCATTCACT AGTACAATTA 
GTACAAGGCC TAAAGGACTT GGTCTGGCTC CCAATAGAGC AGTACCGGAA GGATGGCCGC 
ATTGTCAGAG GGTTTCAGAG AGGCGCTGCT TCCTTTGGTA CCTCGACAGC GATGGCTGCT 
CTAGAACTCA CAAACAGAAT GGTTCAAACC ATACAGGTAA ATTGTAAACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876886.1
CDS	366..6239
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVRGLEMVFRPRPRPATGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTVYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTAPVETEPKLSPSWNPKIIYEPHPQLTRNLPEIAPSDPVQIGRLIGRLELSLTLKQNEVLPGAKLDVDGQIDSIHLLLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSVGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGDPPNMDLELSLTSTYTNTPAGSPLSATVLQPTWGEFLDHHKEQPVRGSTFPSNLVHPTPLQKTSLPSRSVSVDESRPELIFRLAVGTFSISVLHIDPLSPPETSQNLNPLTPMAVAFFTCIEKIDPARFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMEFLECLFPTDFHSVPPHYTELLTFHSKEETGSHSPVCLQLHYKHSENRGPQGNQARLSSVPHKAELQIKLNPVCCELDISIVDRLNSLLQPQKLATVEMMASHMYTSYNKHISLHKAFTEVFLDDSHSPANCRISVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILYLAFTDLEFKTEFIGGSTPEQIKLELTFRELIGSFQEEKGDPSIKFFHVSSGVDGDTTSSDDFDWPRIVLKINPPAMHSILERIAAEEEEENDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQDKAISNSHYVLELTLPNIYVTLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFNKDSFSAFKSAVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLNINHGLIAVFTDVKQDNGDLLENKHGEFWLEFNSGSLFCVTKYEGFDDKHYICLHSSSFSLYHKGIVNGVILPTETRLPSSTRPHWLEPTIYSSEEDGLSKTSSDGVGGDSLNMLSVAVKILSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLSRDYVRVMDMGLLELTITAVKSDSDGEQTEPRFELHCSSDVVHIRTCSDSCAALMNLIQYIASYGDLQTPNKADMKPGAFQRRSKVDSSGRSSSRGPVLPEADQQMLRDLMSDAMEEIDMQQGTSSVKPQANGVLDEKSQIQEPCCSDLFLFPDESGNVSQESGPTYASFSHHFISDAMTGVPTENDDFCILFAPKAAMQEKEEEPVIKIMVDDAIVIRDNYFSLPVNKTDTSKAPLHFPIPVIRYVVKEVSLVWHLYGGKDFGIVPPTSPAKSYISPHSSPSHTPTRHGRNTVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPDCDSSLSEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTVKALHVCPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSAEVELQMTPDPEVKKSPGADVTCSLPRHLSTSKEPNLVISFSGPKQPSQNDSANSVEVVNGMEEKNFSAEEASFRDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLSYRHGLLGVDKLFSYAITEWLNDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQVNCKH

Target ORF information:

RefSeq Version	XM_017021397.2
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy related 2B (ATG2B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021397.2

ORF Insert Sequence:

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GCCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTTCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAAGGCACCG GGTCCCTCGC CCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCTTG 
GAGTCAGCAG ATGCACCCTT AGAAGTCACT GAAGGATTCA TTCAGTCAAT TTCCCTGTCA 
GTTCCATGGG GCTCTTTACT GCAGGATAAT TGTGCACTGG AAGTGAGAGG ATTAGAAATG 
GTCTTCCGGC CTAGACCTCG CCCAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTT 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CCCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAAAAGTCAC TTTTATAGAT ACTGTTTTGA GAATTGAACA TGTGCCAGAA 
AATTCCAAAA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACTGTGTA CTGTGATGAA 
ACTGCTGACG AATCCTCAGG AATTAATGTG CATCAACCCA CTGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCAGC CAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TCTCACCTAG CTGGAACCCC 
AAAATTATTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGAT AGCACCTTCT 
GACCCAGTGC AGATTGGACG GTTAATTGGT AGGTTGGAGT TGAGTCTCAC GTTGAAACAG 
AATGAAGTGC TTCCTGGAGC TAAGTTGGAT GTTGATGGAC AGATAGACTC TATTCATCTA 
CTCCTGTCAC CAAGACAGGT GCACTTGCTT TTGGATATGT TGGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGGA AAAATCGACC CATGCAGCAG 
GAAGACGAGT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTC 
TCTGTGGGTG TATCTTCAGA GCAAAGCTTT TATGAGACAG AAACAGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTATT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA CCCCCCAAAC ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAATA CCCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC CACTTGGGGA 
GAGTTCCTTG ATCATCATAA AGAACAGCCA GTAAGAGGGT CAACATTTCC ATCCAACCTA 
GTTCACCCAA CACCTTTACA GAAGACATCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCAAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACTT TTTCAATCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAAACGTCA CAGAACCTTA ATCCATTGAC ACCTATGGCA 
GTAGCTTTCT TTACTTGTAT AGAAAAGATT GATCCAGCAA GATTTTCAAC AGAAGATTTT 
AAGTCTTTCC GAGCAGTGTT TGCAGAAGCT TGCTCACACG ATCACCTTAG ATTTATAGGT 
ACTGGCATTA AAGTATCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GATATGTCCA TTGGGCAAAT GGAATTTTTG GAGTGCCTAT TTCCAACTGA TTTTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTAACG TTCCATTCCA AAGAAGAAAC TGGTTCCCAT 
TCCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAGAGGGCC CCAGGGTAAT 
CAAGCAAGAC TTAGTTCAGT TCCTCACAAG GCAGAATTGC AAATTAAATT AAATCCAGTG 
TGTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCACAGAAA 
CTTGCCACAG TAGAGATGAT GGCATCCCAC ATGTATACTT CATATAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTGTTTCTA GATGATTCAC ATAGTCCTGC AAATTGTCGG 
ATATCAGTAC AAGTTGCCAC ACCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGATCTG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATCCTT 
TATTTAGCCT TCACAGATCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAA 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAATTGGAT CGTTCCAGGA AGAGAAAGGA 
GATCCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AACATCGTCA 
GATGACTTTG ACTGGCCACG AATTGTACTG AAAATAAATC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TTGCAGCTGA AGAAGAAGAG GAGAATGATG GTCACTACCA GGAGGAAGAG 
GAAGGAGGTG CTCATTCCTT GAAAGATGTT TGTGATCTAA GAAGACCAGC CCCATCTCCT 
TTTTCTTCTC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACAG AATTTCAGGA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACG 
TTACCAAATA TTTATGTAAC ACTACCTAAT AAGAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTTAATG ACTTGCTACT GTGGGAACCA ACAGCTCCTT CACCAGTGGA GACATTCGAG 
AATATTTCCT ATGGCATTGG GCTTTCAGTA GCCAGTCAGC TCATTAATAC TTTCAACAAA 
GATAGTTTTA GTGCATTTAA ATCTGCAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC CACTGTTGAT CCCAACTATC GTTCTCGCAG GAAAAAAAAA 
TTAGACTCTC AGAACAAGAA CTCTCAGAGT TTTCTCTCAG TTCTTCTGAA TATTAATCAT 
GGATTAATAG CAGTGTTCAC AGATGTGAAG CAAGATAATG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTAGA GTTCAATAGT GGTTCATTAT TTTGTGTGAC AAAATATGAA 
GGTTTTGATG ACAAGCACTA CATTTGCCTT CATTCTAGCA GTTTCAGTCT GTACCATAAA 
GGCATAGTGA ATGGAGTGAT TCTCCCGACA GAAACACGAC TTCCCAGCTC AACCCGCCCA 
CACTGGTTGG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTAA AACTTCTTCA 
GATGGAGTTG GAGGAGACAG TTTGAATATG CTGTCTGTTG CCGTTAAAAT ATTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAATTTCTC ATTGCCGTAG GACTGAAAGG AGCCACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTTT GGGATATAAT CCTCCAACTT CATTTACAAC TTTTCATGTT 
CATCTTTGGA GCTGTGCACT TGATTATAGA CCCCTTTATT TGCCAATCCG ATCTCTTCTT 
ACCGTGGAAA CATTCAGTGT TTCCAGTAGC GTTGCATTGG ATAAATCTTC CTCTACTCTC 
AGAATAATCT TGGATGAAGC TGCTTTACAT CTATCTGACA AATGCAATAC TGTCACTATA 
AATCTGAGTA GAGATTATGT TCGTGTGATG GATATGGGGC TTTTGGAGTT AACCATAACT 
GCAGTGAAGT CTGATTCTGA TGGAGAGCAA ACTGAGCCCC GCTTTGAGTT ACACTGTTCC 
AGCGATGTTG TCCATATCAG AACGTGCTCA GACTCTTGTG CTGCGTTAAT GAATCTCATT 
CAGTACATTG CAAGCTATGG TGACTTGCAG ACACCTAACA AGGCAGATAT GAAGCCTGGA 
GCCTTTCAAA GAAGGTCTAA GGTAGATTCC AGTGGTCGAT CATCCTCACG TGGTCCAGTA 
CTTCCTGAAG CAGATCAACA AATGTTACGA GATCTGATGA GTGATGCTAT GGAGGAGATC 
GACATGCAAC AAGGCACCTC GTCAGTAAAA CCACAGGCTA ATGGTGTTTT GGATGAAAAA 
TCTCAAATTC AGGAGCCATG TTGTTCAGAC CTCTTCCTGT TTCCTGACGA GAGTGGGAAT 
GTATCCCAGG AGTCCGGCCC CACCTATGCC TCATTCTCTC ACCATTTCAT CAGTGATGCA 
ATGACAGGTG TGCCCACTGA GAATGATGAC TTTTGCATTC TTTTTGCACC AAAAGCAGCC 
ATGCAGGAGA AGGAAGAAGA ACCAGTTATA AAAATCATGG TTGATGATGC AATTGTGATA 
AGAGACAATT ATTTCAGTCT GCCCGTTAAT AAGACCGATA CGAGCAAAGC CCCCTTACAC 
TTTCCCATTC CTGTGATTCG CTATGTGGTG AAGGAGGTCT CTCTTGTCTG GCATCTTTAT 
GGAGGAAAGG ATTTTGGAAT AGTCCCTCCC ACTTCTCCGG CTAAAAGTTA TATTAGTCCC 
CACAGTTCGC CTTCTCACAC ACCCACGAGA CATGGACGTA ATACAGTATG TGGGGGAAAA 
GGAAGGAACC ATGACTTTTT AATGGAAATA CAGCTAAGCA AGGTGAAGTT TCAGCATGAA 
GTCTACCCGC CATGCAAACC TGATTGTGAT TCCAGCCTCT CAGAACACCC AGTCTCCCGG 
CAGGTGTTCA TTGTTCAGGA TCTTGAGATT CGAGATCGTT TGGCAACATC ACAAATGAAT 
AAATTTTTAT ACCTGTATTG CAGTAAAGAA ATGCCTCGAA AAGCTCACTC CAACATGTTG 
ACAGTGAAAG CCTTACACGT GTGTCCAGAA TCTGGCAGGT CCCCACAGGA GTGCTGCTTG 
AGAGTGTCGC TGATGCCGCT CCGCCTCAAT ATTGACCAGG ATGCTTTGTT CTTCCTGAAG 
GATTTCTTCA CAAGTCTTTC TGCAGAAGTA GAGCTTCAAA TGACTCCAGA TCCAGAAGTT 
AAAAAGTCTC CTGGAGCTGA TGTCACCTGC AGTTTGCCAA GGCATTTGAG TACCTCAAAG 
GAGCCAAATC TGGTTATTTC TTTCTCTGGG CCAAAACAGC CTTCCCAAAA TGATAGTGCC 
AATTCAGTGG AAGTGGTTAA TGGCATGGAA GAGAAGAACT TCTCTGCTGA AGAAGCATCT 
TTTAGGGATC AGCCTGTGTT TTTTAGAGAA TTTAGATTCA CGTCAGAAGT TCCCATTCGA 
CTTGATTATC ATGGCAAACA TGTATCAATG GATCAGGGTA CGCTAGCTGG GATTTTGATT 
GGTCTGGCTC AGTTAAACTG CTCTGAACTA AAGCTCAAGA GGCTTTCCTA TCGACATGGT 
TTACTAGGCG TTGACAAATT ATTCTCATAT GCAATCACTG AGTGGCTTAA TGACATTAAG 
AAGAACCAGC TACCAGGAAT CCTGGGAGGT GTTGGACCTA TGCATTCACT AGTACAATTA 
GTACAAGGCC TAAAGGACTT GGTCTGGCTC CCAATAGAGC AGTACCGGAA GGATGGCCGC 
ATTGTCAGAG GGTTTCAGAG AGGCGCTGCT TCCTTTGGTA CCTCGACAGC GATGGCTGCT 
CTAGAACTCA CAAACAGAAT GGTTCAAACC ATACAGGTAA ATTGTAAACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( NM_018036.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_060506.5 Homo sapiens autophagy related 2B (ATG2B), mRNA.

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CloneID	OHu02652
Clone ID Related Accession (Same CDS sequence)	NM_018036.6
Accession Version	NM_018036.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Homo sapiens(human)
Product	autophagy-related protein 2 homolog B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL359240.4, AK055200.1, BU166120.1, BU101822.1, BU167202.1, BC015851.1, AK128275.1, BC015016.2, AL355102.5 and AW264403.1. This sequence is a reference standard in the RefSeqGene project. On Jul 8, 2016 this sequence version replaced NM_018036.5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.80978.1, SRR1803612.275299.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GCCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTTCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAAGGCACCG GGTCCCTCGC CCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCTTG 
GAGTCAGCAG ATGCACCCTT AGAAGTCACT GAAGGATTCA TTCAGTCAAT TTCCCTGTCA 
GTTCCATGGG GCTCTTTACT GCAGGATAAT TGTGCACTGG AAGTGAGAGG ATTAGAAATG 
GTCTTCCGGC CTAGACCTCG CCCAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTT 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CCCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAAAAGTCAC TTTTATAGAT ACTGTTTTGA GAATTGAACA TGTGCCAGAA 
AATTCCAAAA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACTGTGTA CTGTGATGAA 
ACTGCTGACG AATCCTCAGG AATTAATGTG CATCAACCCA CTGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCAGC CAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TCTCACCTAG CTGGAACCCC 
AAAATTATTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGAT AGCACCTTCT 
GACCCAGTGC AGATTGGACG GTTAATTGGT AGGTTGGAGT TGAGTCTCAC GTTGAAACAG 
AATGAAGTGC TTCCTGGAGC TAAGTTGGAT GTTGATGGAC AGATAGACTC TATTCATCTA 
CTCCTGTCAC CAAGACAGGT GCACTTGCTT TTGGATATGT TGGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGGA AAAATCGACC CATGCAGCAG 
GAAGACGAGT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTC 
TCTGTGGGTG TATCTTCAGA GCAAAGCTTT TATGAGACAG AAACAGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTATT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA CCCCCCAAAC ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAATA CCCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC CACTTGGGGA 
GAGTTCCTTG ATCATCATAA AGAACAGCCA GTAAGAGGGT CAACATTTCC ATCCAACCTA 
GTTCACCCAA CACCTTTACA GAAGACATCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCAAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACTT TTTCAATCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAAACGTCA CAGAACCTTA ATCCATTGAC ACCTATGGCA 
GTAGCTTTCT TTACTTGTAT AGAAAAGATT GATCCAGCAA GATTTTCAAC AGAAGATTTT 
AAGTCTTTCC GAGCAGTGTT TGCAGAAGCT TGCTCACACG ATCACCTTAG ATTTATAGGT 
ACTGGCATTA AAGTATCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GATATGTCCA TTGGGCAAAT GGAATTTTTG GAGTGCCTAT TTCCAACTGA TTTTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTAACG TTCCATTCCA AAGAAGAAAC TGGTTCCCAT 
TCCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAGAGGGCC CCAGGGTAAT 
CAAGCAAGAC TTAGTTCAGT TCCTCACAAG GCAGAATTGC AAATTAAATT AAATCCAGTG 
TGTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCACAGAAA 
CTTGCCACAG TAGAGATGAT GGCATCCCAC ATGTATACTT CATATAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTGTTTCTA GATGATTCAC ATAGTCCTGC AAATTGTCGG 
ATATCAGTAC AAGTTGCCAC ACCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGATCTG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATCCTT 
TATTTAGCCT TCACAGATCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAA 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAATTGGAT CGTTCCAGGA AGAGAAAGGA 
GATCCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AACATCGTCA 
GATGACTTTG ACTGGCCACG AATTGTACTG AAAATAAATC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TTGCAGCTGA AGAAGAAGAG GAGAATGATG GTCACTACCA GGAGGAAGAG 
GAAGGAGGTG CTCATTCCTT GAAAGATGTT TGTGATCTAA GAAGACCAGC CCCATCTCCT 
TTTTCTTCTC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACAG AATTTCAGGA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACG 
TTACCAAATA TTTATGTAAC ACTACCTAAT AAGAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTTAATG ACTTGCTACT GTGGGAACCA ACAGCTCCTT CACCAGTGGA GACATTCGAG 
AATATTTCCT ATGGCATTGG GCTTTCAGTA GCCAGTCAGC TCATTAATAC TTTCAACAAA 
GATAGTTTTA GTGCATTTAA ATCTGCAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC CACTGTTGAT CCCAACTATC GTTCTCGCAG GAAAAAAAAA 
TTAGACTCTC AGAACAAGAA CTCTCAGAGT TTTCTCTCAG TTCTTCTGAA TATTAATCAT 
GGATTAATAG CAGTGTTCAC AGATGTGAAG CAAGATAATG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTAGA GTTCAATAGT GGTTCATTAT TTTGTGTGAC AAAATATGAA 
GGTTTTGATG ACAAGCACTA CATTTGCCTT CATTCTAGCA GTTTCAGTCT GTACCATAAA 
GGCATAGTGA ATGGAGTGAT TCTCCCGACA GAAACACGAC TTCCCAGCTC AACCCGCCCA 
CACTGGTTGG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTAA AACTTCTTCA 
GATGGAGTTG GAGGAGACAG TTTGAATATG CTGTCTGTTG CCGTTAAAAT ATTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAATTTCTC ATTGCCGTAG GACTGAAAGG AGCCACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTTT GGGATATAAT CCTCCAACTT CATTTACAAC TTTTCATGTT 
CATCTTTGGA GCTGTGCACT TGATTATAGA CCCCTTTATT TGCCAATCCG ATCTCTTCTT 
ACCGTGGAAA CATTCAGTGT TTCCAGTAGC GTTGCATTGG ATAAATCTTC CTCTACTCTC 
AGAATAATCT TGGATGAAGC TGCTTTACAT CTATCTGACA AATGCAATAC TGTCACTATA 
AATCTGAGTA GAGATTATGT TCGTGTGATG GATATGGGGC TTTTGGAGTT AACCATAACT 
GCAGTGAAGT CTGATTCTGA TGGAGAGCAA ACTGAGCCCC GCTTTGAGTT ACACTGTTCC 
AGCGATGTTG TCCATATCAG AACGTGCTCA GACTCTTGTG CTGCGTTAAT GAATCTCATT 
CAGTACATTG CAAGCTATGG TGACTTGCAG ACACCTAACA AGGCAGATAT GAAGCCTGGA 
GCCTTTCAAA GAAGGTCTAA GGTAGATTCC AGTGGTCGAT CATCCTCACG TGGTCCAGTA 
CTTCCTGAAG CAGATCAACA AATGTTACGA GATCTGATGA GTGATGCTAT GGAGGAGATC 
GACATGCAAC AAGGCACCTC GTCAGTAAAA CCACAGGCTA ATGGTGTTTT GGATGAAAAA 
TCTCAAATTC AGGAGCCATG TTGTTCAGAC CTCTTCCTGT TTCCTGACGA GAGTGGGAAT 
GTATCCCAGG AGTCCGGCCC CACCTATGCC TCATTCTCTC ACCATTTCAT CAGTGATGCA 
ATGACAGGTG TGCCCACTGA GAATGATGAC TTTTGCATTC TTTTTGCACC AAAAGCAGCC 
ATGCAGGAGA AGGAAGAAGA ACCAGTTATA AAAATCATGG TTGATGATGC AATTGTGATA 
AGAGACAATT ATTTCAGTCT GCCCGTTAAT AAGACCGATA CGAGCAAAGC CCCCTTACAC 
TTTCCCATTC CTGTGATTCG CTATGTGGTG AAGGAGGTCT CTCTTGTCTG GCATCTTTAT 
GGAGGAAAGG ATTTTGGAAC AGTCCCTCCC ACTTCTCCGG CTAAAAGTTA TATTAGTCCC 
CACAGTTCGC CTTCTCACAC ACCCACGAGA CATGGACGTA ATACAGTATG TGGGGGAAAA 
GGAAGGAACC ATGACTTTTT AATGGAAATA CAGCTAAGCA AGGTGAAGTT TCAGCATGAA 
GTCTACCCGC CATGCAAACC TGATTGTGAT TCCAGCCTCT CAGAACACCC AGTCTCCCGG 
CAGGTGTTCA TTGTTCAGGA TCTTGAGATT CGAGATCGTT TGGCAACATC ACAAATGAAT 
AAATTTTTAT ACCTGTATTG CAGTAAAGAA ATGCCTCGAA AAGCTCACTC CAACATGTTG 
ACAGTGAAAG CCTTACACGT GTGTCCAGAA TCTGGCAGGT CCCCACAGGA GTGCTGCTTG 
AGAGTGTCGC TGATGCCGCT CCGCCTCAAT ATTGACCAGG ATGCTTTGTT CTTCCTGAAG 
GATTTCTTCA CAAGTCTTTC TGCAGAAGTA GAGCTTCAAA TGACTCCAGA TCCAGAAGTT 
AAAAAGTCTC CTGGAGCTGA TGTCACCTGC AGTTTGCCAA GGCATTTGAG TACCTCAAAG 
GAGCCAAATC TGGTTATTTC TTTCTCTGGG CCAAAACAGC CTTCCCAAAA TGATAGTGCC 
AATTCAGTGG AAGTGGTTAA TGGCATGGAA GAGAAGAACT TCTCTGCTGA AGAAGCATCT 
TTTAGGGATC AGCCTGTGTT TTTTAGAGAA TTTAGATTCA CGTCAGAAGT TCCCATTCGA 
CTTGATTATC ATGGCAAACA TGTATCAATG GATCAGGGTA CGCTAGCTGG GATTTTGATT 
GGTCTGGCTC AGTTAAACTG CTCTGAACTA AAGCTCAAGA GGCTTTCCTA TCGACATGGT 
TTACTAGGCG TTGACAAATT ATTCTCATAT GCAATCACTG AGTGGCTTAA TGACATTAAG 
AAGAACCAGC TACCAGGAAT CCTGGGAGGT GTTGGACCTA TGCATTCACT AGTACAATTA 
GTACAAGGCC TAAAGGACTT GGTCTGGCTC CCAATAGAGC AGTACCGGAA GGATGGCCGC 
ATTGTCAGAG GGTTTCAGAG AGGCGCTGCT TCCTTTGGTA CCTCGACAGC GATGGCTGCT 
CTAGAACTCA CAAACAGAAT GGTTCAAACC ATACAGGCAG CTGCAGAGAC TGCTTATGAT 
ATGGTGTCTC CTGGTACCCT TTCTATCGAG CCCAAGAAGA CCAAAAGGTT TCCTCATCAC 
CGGTTAGCCC ACCAGCCAGT AGACCTGAGG GAAGGTGTGG CCAAGGCCTA CAGTGTTGTG 
AAAGAGGGAA TCACAGACAC GGCTCAGACC ATTTATGAAA CTGCGGCTCG AGAACACGAG 
AGCAGAGGGG TGACTGGTGC CGTGGGCGAG GTTCTGCGCC AGATTCCTCC GGCAGTGGTG 
AAACCTCTGA TTGTTGCCAC AGAAGCAACG TCAAACGTGC TGGGTGGCAT GAGAAACCAA 
ATTAGGCCAG ATGTCCGGCA AGACGAGTCA CAGAAATGGC GCCACGGGGA TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060506.5
CDS	426..6662
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVRGLEMVFRPRPRPATGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTVYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTAPVETEPKLSPSWNPKIIYEPHPQLTRNLPEIAPSDPVQIGRLIGRLELSLTLKQNEVLPGAKLDVDGQIDSIHLLLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSVGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGDPPNMDLELSLTSTYTNTPAGSPLSATVLQPTWGEFLDHHKEQPVRGSTFPSNLVHPTPLQKTSLPSRSVSVDESRPELIFRLAVGTFSISVLHIDPLSPPETSQNLNPLTPMAVAFFTCIEKIDPARFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMEFLECLFPTDFHSVPPHYTELLTFHSKEETGSHSPVCLQLHYKHSENRGPQGNQARLSSVPHKAELQIKLNPVCCELDISIVDRLNSLLQPQKLATVEMMASHMYTSYNKHISLHKAFTEVFLDDSHSPANCRISVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILYLAFTDLEFKTEFIGGSTPEQIKLELTFRELIGSFQEEKGDPSIKFFHVSSGVDGDTTSSDDFDWPRIVLKINPPAMHSILERIAAEEEEENDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQDKAISNSHYVLELTLPNIYVTLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFNKDSFSAFKSAVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLNINHGLIAVFTDVKQDNGDLLENKHGEFWLEFNSGSLFCVTKYEGFDDKHYICLHSSSFSLYHKGIVNGVILPTETRLPSSTRPHWLEPTIYSSEEDGLSKTSSDGVGGDSLNMLSVAVKILSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLSRDYVRVMDMGLLELTITAVKSDSDGEQTEPRFELHCSSDVVHIRTCSDSCAALMNLIQYIASYGDLQTPNKADMKPGAFQRRSKVDSSGRSSSRGPVLPEADQQMLRDLMSDAMEEIDMQQGTSSVKPQANGVLDEKSQIQEPCCSDLFLFPDESGNVSQESGPTYASFSHHFISDAMTGVPTENDDFCILFAPKAAMQEKEEEPVIKIMVDDAIVIRDNYFSLPVNKTDTSKAPLHFPIPVIRYVVKEVSLVWHLYGGKDFGTVPPTSPAKSYISPHSSPSHTPTRHGRNTVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPDCDSSLSEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTVKALHVCPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSAEVELQMTPDPEVKKSPGADVTCSLPRHLSTSKEPNLVISFSGPKQPSQNDSANSVEVVNGMEEKNFSAEEASFRDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLSYRHGLLGVDKLFSYAITEWLNDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKTKRFPHHRLAHQPVDLREGVAKAYSVVKEGITDTAQTIYETAAREHESRGVTGAVGEVLRQIPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGDD

Target ORF information:

RefSeq Version	NM_018036.6
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy related 2B (ATG2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018036.6

ORF Insert Sequence:

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GCCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTTCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAAGGCACCG GGTCCCTCGC CCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCTTG 
GAGTCAGCAG ATGCACCCTT AGAAGTCACT GAAGGATTCA TTCAGTCAAT TTCCCTGTCA 
GTTCCATGGG GCTCTTTACT GCAGGATAAT TGTGCACTGG AAGTGAGAGG ATTAGAAATG 
GTCTTCCGGC CTAGACCTCG CCCAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTT 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CCCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAAAAGTCAC TTTTATAGAT ACTGTTTTGA GAATTGAACA TGTGCCAGAA 
AATTCCAAAA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACTGTGTA CTGTGATGAA 
ACTGCTGACG AATCCTCAGG AATTAATGTG CATCAACCCA CTGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCAGC CAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TCTCACCTAG CTGGAACCCC 
AAAATTATTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGAT AGCACCTTCT 
GACCCAGTGC AGATTGGACG GTTAATTGGT AGGTTGGAGT TGAGTCTCAC GTTGAAACAG 
AATGAAGTGC TTCCTGGAGC TAAGTTGGAT GTTGATGGAC AGATAGACTC TATTCATCTA 
CTCCTGTCAC CAAGACAGGT GCACTTGCTT TTGGATATGT TGGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGGA AAAATCGACC CATGCAGCAG 
GAAGACGAGT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTC 
TCTGTGGGTG TATCTTCAGA GCAAAGCTTT TATGAGACAG AAACAGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTATT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CCCTTGGGGA CCCCCCAAAC ATGGACCTTG AGTTATCATT AACTAGTACA 
TATACAAATA CCCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC CACTTGGGGA 
GAGTTCCTTG ATCATCATAA AGAACAGCCA GTAAGAGGGT CAACATTTCC ATCCAACCTA 
GTTCACCCAA CACCTTTACA GAAGACATCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCAAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACTT TTTCAATCTC TGTGCTTCAC 
ATTGATCCTT TATCTCCACC TGAAACGTCA CAGAACCTTA ATCCATTGAC ACCTATGGCA 
GTAGCTTTCT TTACTTGTAT AGAAAAGATT GATCCAGCAA GATTTTCAAC AGAAGATTTT 
AAGTCTTTCC GAGCAGTGTT TGCAGAAGCT TGCTCACACG ATCACCTTAG ATTTATAGGT 
ACTGGCATTA AAGTATCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GATATGTCCA TTGGGCAAAT GGAATTTTTG GAGTGCCTAT TTCCAACTGA TTTTCATTCT 
GTTCCTCCTC ACTATACAGA GCTTTTAACG TTCCATTCCA AAGAAGAAAC TGGTTCCCAT 
TCCCCTGTGT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAGAGGGCC CCAGGGTAAT 
CAAGCAAGAC TTAGTTCAGT TCCTCACAAG GCAGAATTGC AAATTAAATT AAATCCAGTG 
TGTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTTCA ACCACAGAAA 
CTTGCCACAG TAGAGATGAT GGCATCCCAC ATGTATACTT CATATAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTGTTTCTA GATGATTCAC ATAGTCCTGC AAATTGTCGG 
ATATCAGTAC AAGTTGCCAC ACCAGCATTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGATCTG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATCCTT 
TATTTAGCCT TCACAGATCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAA 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAATTGGAT CGTTCCAGGA AGAGAAAGGA 
GATCCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AACATCGTCA 
GATGACTTTG ACTGGCCACG AATTGTACTG AAAATAAATC CACCAGCCAT GCATTCCATT 
TTGGAGAGAA TTGCAGCTGA AGAAGAAGAG GAGAATGATG GTCACTACCA GGAGGAAGAG 
GAAGGAGGTG CTCATTCCTT GAAAGATGTT TGTGATCTAA GAAGACCAGC CCCATCTCCT 
TTTTCTTCTC GTAGAGTAAT GTTTGAAAAT GAACAGATGG TGATGCCAGG AGACCCTGTA 
GAAATGACAG AATTTCAGGA TAAAGCAATC AGCAATTCTC ACTATGTGCT GGAACTTACG 
TTACCAAATA TTTATGTAAC ACTACCTAAT AAGAGCTTTT ATGAGAAGCT TTATAATAGG 
ATCTTTAATG ACTTGCTACT GTGGGAACCA ACAGCTCCTT CACCAGTGGA GACATTCGAG 
AATATTTCCT ATGGCATTGG GCTTTCAGTA GCCAGTCAGC TCATTAATAC TTTCAACAAA 
GATAGTTTTA GTGCATTTAA ATCTGCAGTT CACTATGATG AGGAAAGTGG ATCTGAGGAG 
GAGACTTTGC AGTATTTTTC CACTGTTGAT CCCAACTATC GTTCTCGCAG GAAAAAAAAA 
TTAGACTCTC AGAACAAGAA CTCTCAGAGT TTTCTCTCAG TTCTTCTGAA TATTAATCAT 
GGATTAATAG CAGTGTTCAC AGATGTGAAG CAAGATAATG GAGATCTGTT GGAAAACAAG 
CATGGTGAAT TCTGGTTAGA GTTCAATAGT GGTTCATTAT TTTGTGTGAC AAAATATGAA 
GGTTTTGATG ACAAGCACTA CATTTGCCTT CATTCTAGCA GTTTCAGTCT GTACCATAAA 
GGCATAGTGA ATGGAGTGAT TCTCCCGACA GAAACACGAC TTCCCAGCTC AACCCGCCCA 
CACTGGTTGG AACCTACTAT TTATTCCTCT GAAGAAGATG GCCTCAGTAA AACTTCTTCA 
GATGGAGTTG GAGGAGACAG TTTGAATATG CTGTCTGTTG CCGTTAAAAT ATTGTCTGAT 
AAATCAGAGT CCAATACAAA GGAATTTCTC ATTGCCGTAG GACTGAAAGG AGCCACTCTC 
CAGCATAGAA TGCTTCCTTC TGGGCTTAGC TGGCATGAGC AGATTTTATA CTTCTTGAAT 
ATTGCTGATG AACCTGTTTT GGGATATAAT CCTCCAACTT CATTTACAAC TTTTCATGTT 
CATCTTTGGA GCTGTGCACT TGATTATAGA CCCCTTTATT TGCCAATCCG ATCTCTTCTT 
ACCGTGGAAA CATTCAGTGT TTCCAGTAGC GTTGCATTGG ATAAATCTTC CTCTACTCTC 
AGAATAATCT TGGATGAAGC TGCTTTACAT CTATCTGACA AATGCAATAC TGTCACTATA 
AATCTGAGTA GAGATTATGT TCGTGTGATG GATATGGGGC TTTTGGAGTT AACCATAACT 
GCAGTGAAGT CTGATTCTGA TGGAGAGCAA ACTGAGCCCC GCTTTGAGTT ACACTGTTCC 
AGCGATGTTG TCCATATCAG AACGTGCTCA GACTCTTGTG CTGCGTTAAT GAATCTCATT 
CAGTACATTG CAAGCTATGG TGACTTGCAG ACACCTAACA AGGCAGATAT GAAGCCTGGA 
GCCTTTCAAA GAAGGTCTAA GGTAGATTCC AGTGGTCGAT CATCCTCACG TGGTCCAGTA 
CTTCCTGAAG CAGATCAACA AATGTTACGA GATCTGATGA GTGATGCTAT GGAGGAGATC 
GACATGCAAC AAGGCACCTC GTCAGTAAAA CCACAGGCTA ATGGTGTTTT GGATGAAAAA 
TCTCAAATTC AGGAGCCATG TTGTTCAGAC CTCTTCCTGT TTCCTGACGA GAGTGGGAAT 
GTATCCCAGG AGTCCGGCCC CACCTATGCC TCATTCTCTC ACCATTTCAT CAGTGATGCA 
ATGACAGGTG TGCCCACTGA GAATGATGAC TTTTGCATTC TTTTTGCACC AAAAGCAGCC 
ATGCAGGAGA AGGAAGAAGA ACCAGTTATA AAAATCATGG TTGATGATGC AATTGTGATA 
AGAGACAATT ATTTCAGTCT GCCCGTTAAT AAGACCGATA CGAGCAAAGC CCCCTTACAC 
TTTCCCATTC CTGTGATTCG CTATGTGGTG AAGGAGGTCT CTCTTGTCTG GCATCTTTAT 
GGAGGAAAGG ATTTTGGAAC AGTCCCTCCC ACTTCTCCGG CTAAAAGTTA TATTAGTCCC 
CACAGTTCGC CTTCTCACAC ACCCACGAGA CATGGACGTA ATACAGTATG TGGGGGAAAA 
GGAAGGAACC ATGACTTTTT AATGGAAATA CAGCTAAGCA AGGTGAAGTT TCAGCATGAA 
GTCTACCCGC CATGCAAACC TGATTGTGAT TCCAGCCTCT CAGAACACCC AGTCTCCCGG 
CAGGTGTTCA TTGTTCAGGA TCTTGAGATT CGAGATCGTT TGGCAACATC ACAAATGAAT 
AAATTTTTAT ACCTGTATTG CAGTAAAGAA ATGCCTCGAA AAGCTCACTC CAACATGTTG 
ACAGTGAAAG CCTTACACGT GTGTCCAGAA TCTGGCAGGT CCCCACAGGA GTGCTGCTTG 
AGAGTGTCGC TGATGCCGCT CCGCCTCAAT ATTGACCAGG ATGCTTTGTT CTTCCTGAAG 
GATTTCTTCA CAAGTCTTTC TGCAGAAGTA GAGCTTCAAA TGACTCCAGA TCCAGAAGTT 
AAAAAGTCTC CTGGAGCTGA TGTCACCTGC AGTTTGCCAA GGCATTTGAG TACCTCAAAG 
GAGCCAAATC TGGTTATTTC TTTCTCTGGG CCAAAACAGC CTTCCCAAAA TGATAGTGCC 
AATTCAGTGG AAGTGGTTAA TGGCATGGAA GAGAAGAACT TCTCTGCTGA AGAAGCATCT 
TTTAGGGATC AGCCTGTGTT TTTTAGAGAA TTTAGATTCA CGTCAGAAGT TCCCATTCGA 
CTTGATTATC ATGGCAAACA TGTATCAATG GATCAGGGTA CGCTAGCTGG GATTTTGATT 
GGTCTGGCTC AGTTAAACTG CTCTGAACTA AAGCTCAAGA GGCTTTCCTA TCGACATGGT 
TTACTAGGCG TTGACAAATT ATTCTCATAT GCAATCACTG AGTGGCTTAA TGACATTAAG 
AAGAACCAGC TACCAGGAAT CCTGGGAGGT GTTGGACCTA TGCATTCACT AGTACAATTA 
GTACAAGGCC TAAAGGACTT GGTCTGGCTC CCAATAGAGC AGTACCGGAA GGATGGCCGC 
ATTGTCAGAG GGTTTCAGAG AGGCGCTGCT TCCTTTGGTA CCTCGACAGC GATGGCTGCT 
CTAGAACTCA CAAACAGAAT GGTTCAAACC ATACAGGCAG CTGCAGAGAC TGCTTATGAT 
ATGGTGTCTC CTGGTACCCT TTCTATCGAG CCCAAGAAGA CCAAAAGGTT TCCTCATCAC 
CGGTTAGCCC ACCAGCCAGT AGACCTGAGG GAAGGTGTGG CCAAGGCCTA CAGTGTTGTG 
AAAGAGGGAA TCACAGACAC GGCTCAGACC ATTTATGAAA CTGCGGCTCG AGAACACGAG 
AGCAGAGGGG TGACTGGTGC CGTGGGCGAG GTTCTGCGCC AGATTCCTCC GGCAGTGGTG 
AAACCTCTGA TTGTTGCCAC AGAAGCAACG TCAAACGTGC TGGGTGGCAT GAGAAACCAA 
ATTAGGCCAG ATGTCCGGCA AGACGAGTCA CAGAAATGGC GCCACGGGGA TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_006720187.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006720250.1 Homo sapiens autophagy related 2B (ATG2B), transcript variant X1, mRNA.

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CloneID	OHu47763
Clone ID Related Accession (Same CDS sequence)	XM_006720187.2
Accession Version	XM_006720187.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	autophagy-related protein 2 homolog B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006720187.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GCCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTTCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAAGGCACCG GGTCCCTCGC CCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCTTG 
GAGTCAGCAG ATGCACCCTT AGAAGTCACT GAAGGATTCA TTCAGTCAAT TTCCCTGTCA 
GTTCCATGGG GCTCTTTACT GCAGGATAAT TGTGCACTGG AAGTGAGAGG ATTAGAAATG 
GTCTTCCGGC CTAGACCTCG CCCAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTT 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CCCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAAAAGTCAC TTTTATAGAT ACTGTTTTGA GAATTGAACA TGTGCCAGAA 
AATTCCAAAA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACTGTGTA CTGTGATGAA 
ACTGCTGACG AATCCTCAGG AATTAATGTG CATCAACCCA CTGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCAGC CAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TCTCACCTAG CTGGAACCCC 
AAAATTATTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGAT AGCACCTTCT 
GACCCAGTGC AGATTGGACG GTTAATTGGT AGGTTGGAGT TGAGTCTCAC GTTGAAACAG 
AATGAAGTGC TTCCTGGAGC TAAGTTGGAT GTTGATGGAC AGATAGACTC TATTCATCTA 
CTCCTGTCAC CAAGACAGGT GCACTTGCTT TTGGATATGT TGGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGGA AAAATCGACC CATGCAGCAG 
GAAGACGAGT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTC 
TCTGTGGGTG TATCTTCAGA GCAAAGCTTT TATGAGACAG AAACAGCTCG TACACCTTCT 
AGCCGTGAAG AAGTATTCTT CTCCATGGCT GATATGGACA TGTCTCATAG TCTCTCTTCT 
CTTCCACCCC TTGGGGACCC CCCAAACATG GACCTTGAGT TATCATTAAC TAGTACATAT 
ACAAATACCC CAGCAGGATC TCCATTAAGT GCTACTGTGC TTCAGCCCAC TTGGGGAGAG 
TTCCTTGATC ATCATAAAGA ACAGCCAGTA AGAGGGTCAA CATTTCCATC CAACCTAGTT 
CACCCAACAC CTTTACAGAA GACATCTCTC CCATCTAGAT CTGTTTCAGT GGATGAATCA 
AGGCCTGAAC TTATTTTTAG ACTAGCTGTG GGAACTTTTT CAATCTCTGT GCTTCACATT 
GATCCTTTAT CTCCACCTGA AACGTCACAG AACCTTAATC CATTGACACC TATGGCAGTA 
GCTTTCTTTA CTTGTATAGA AAAGATTGAT CCAGCAAGAT TTTCAACAGA AGATTTTAAG 
TCTTTCCGAG CAGTGTTTGC AGAAGCTTGC TCACACGATC ACCTTAGATT TATAGGTACT 
GGCATTAAAG TATCCTATGA ACAAAGACAA AGATCAGCTT CCCGATATTT TAGTACTGAT 
ATGTCCATTG GGCAAATGGA ATTTTTGGAG TGCCTATTTC CAACTGATTT TCATTCTGTT 
CCTCCTCACT ATACAGAGCT TTTAACGTTC CATTCCAAAG AAGAAACTGG TTCCCATTCC 
CCTGTGTGTC TTCAGCTTCA TTATAAGCAT TCTGAGAATA GAGGGCCCCA GGGTAATCAA 
GCAAGACTTA GTTCAGTTCC TCACAAGGCA GAATTGCAAA TTAAATTAAA TCCAGTGTGT 
TGTGAGCTGG ATATCAGTAT TGTGGACAGG TTAAATTCCT TGCTTCAACC ACAGAAACTT 
GCCACAGTAG AGATGATGGC ATCCCACATG TATACTTCAT ATAATAAACA TATTAGTCTG 
CACAAGGCTT TCACTGAAGT GTTTCTAGAT GATTCACATA GTCCTGCAAA TTGTCGGATA 
TCAGTACAAG TTGCCACACC AGCATTAAAC CTTTCTGTTC GCTTCCCAAT ACCTGATCTT 
CGATCTGATC AAGAAAGAGG ACCATGGTTT AAGAAGTCAC TTCAGAAGGA GATCCTTTAT 
TTAGCCTTCA CAGATCTAGA ATTTAAGACT GAATTTATAG GAGGATCAAC CCCAGAACAA 
ATTAAATTGG AACTTACCTT TAGAGAACTA ATTGGATCGT TCCAGGAAGA GAAAGGAGAT 
CCATCTATTA AGTTTTTCCA TGTGTCTAGT GGAGTAGATG GAGATACAAC ATCGTCAGAT 
GACTTTGACT GGCCACGAAT TGTACTGAAA ATAAATCCAC CAGCCATGCA TTCCATTTTG 
GAGAGAATTG CAGCTGAAGA AGAAGAGGAG AATGATGGTC ACTACCAGGA GGAAGAGGAA 
GGAGGTGCTC ATTCCTTGAA AGATGTTTGT GATCTAAGAA GACCAGCCCC ATCTCCTTTT 
TCTTCTCGTA GAGTAATGTT TGAAAATGAA CAGATGGTGA TGCCAGGAGA CCCTGTAGAA 
ATGACAGAAT TTCAGGATAA AGCAATCAGC AATTCTCACT ATGTGCTGGA ACTTACGTTA 
CCAAATATTT ATGTAACACT ACCTAATAAG AGCTTTTATG AGAAGCTTTA TAATAGGATC 
TTTAATGACT TGCTACTGTG GGAACCAACA GCTCCTTCAC CAGTGGAGAC ATTCGAGAAT 
ATTTCCTATG GCATTGGGCT TTCAGTAGCC AGTCAGCTCA TTAATACTTT CAACAAAGAT 
AGTTTTAGTG CATTTAAATC TGCAGTTCAC TATGATGAGG AAAGTGGATC TGAGGAGGAG 
ACTTTGCAGT ATTTTTCCAC TGTTGATCCC AACTATCGTT CTCGCAGGAA AAAAAAATTA 
GACTCTCAGA ACAAGAACTC TCAGAGTTTT CTCTCAGTTC TTCTGAATAT TAATCATGGA 
TTAATAGCAG TGTTCACAGA TGTGAAGCAA GATAATGGAG ATCTGTTGGA AAACAAGCAT 
GGTGAATTCT GGTTAGAGTT CAATAGTGGT TCATTATTTT GTGTGACAAA ATATGAAGGT 
TTTGATGACA AGCACTACAT TTGCCTTCAT TCTAGCAGTT TCAGTCTGTA CCATAAAGGC 
ATAGTGAATG GAGTGATTCT CCCGACAGAA ACACGACTTC CCAGCTCAAC CCGCCCACAC 
TGGTTGGAAC CTACTATTTA TTCCTCTGAA GAAGATGGCC TCAGTAAAAC TTCTTCAGAT 
GGAGTTGGAG GAGACAGTTT GAATATGCTG TCTGTTGCCG TTAAAATATT GTCTGATAAA 
TCAGAGTCCA ATACAAAGGA ATTTCTCATT GCCGTAGGAC TGAAAGGAGC CACTCTCCAG 
CATAGAATGC TTCCTTCTGG GCTTAGCTGG CATGAGCAGA TTTTATACTT CTTGAATATT 
GCTGATGAAC CTGTTTTGGG ATATAATCCT CCAACTTCAT TTACAACTTT TCATGTTCAT 
CTTTGGAGCT GTGCACTTGA TTATAGACCC CTTTATTTGC CAATCCGATC TCTTCTTACC 
GTGGAAACAT TCAGTGTTTC CAGTAGCGTT GCATTGGATA AATCTTCCTC TACTCTCAGA 
ATAATCTTGG ATGAAGCTGC TTTACATCTA TCTGACAAAT GCAATACTGT CACTATAAAT 
CTGAGTAGAG ATTATGTTCG TGTGATGGAT ATGGGGCTTT TGGAGTTAAC CATAACTGCA 
GTGAAGTCTG ATTCTGATGG AGAGCAAACT GAGCCCCGCT TTGAGTTACA CTGTTCCAGC 
GATGTTGTCC ATATCAGAAC GTGCTCAGAC TCTTGTGCTG CGTTAATGAA TCTCATTCAG 
TACATTGCAA GCTATGGTGA CTTGCAGACA CCTAACAAGG CAGATATGAA GCCTGGAGCC 
TTTCAAAGAA GGTCTAAGGT AGATTCCAGT GGTCGATCAT CCTCACGTGG TCCAGTACTT 
CCTGAAGCAG ATCAACAAAT GTTACGAGAT CTGATGAGTG ATGCTATGGA GGAGATCGAC 
ATGCAACAAG GCACCTCGTC AGTAAAACCA CAGGCTAATG GTGTTTTGGA TGAAAAATCT 
CAAATTCAGG AGCCATGTTG TTCAGACCTC TTCCTGTTTC CTGACGAGAG TGGGAATGTA 
TCCCAGGAGT CCGGCCCCAC CTATGCCTCA TTCTCTCACC ATTTCATCAG TGATGCAATG 
ACAGGTGTGC CCACTGAGAA TGATGACTTT TGCATTCTTT TTGCACCAAA AGCAGCCATG 
CAGGAGAAGG AAGAAGAACC AGTTATAAAA ATCATGGTTG ATGATGCAAT TGTGATAAGA 
GACAATTATT TCAGTCTGCC CGTTAATAAG ACCGATACGA GCAAAGCCCC CTTACACTTT 
CCCATTCCTG TGATTCGCTA TGTGGTGAAG GAGGTCTCTC TTGTCTGGCA TCTTTATGGA 
GGAAAGGATT TTGGAATAGT CCCTCCCACT TCTCCGGCTA AAAGTTATAT TAGTCCCCAC 
AGTTCGCCTT CTCACACACC CACGAGACAT GGACGTAATA CAGTATGTGG GGGAAAAGGA 
AGGAACCATG ACTTTTTAAT GGAAATACAG CTAAGCAAGG TGAAGTTTCA GCATGAAGTC 
TACCCGCCAT GCAAACCTGA TTGTGATTCC AGCCTCTCAG AACACCCAGT CTCCCGGCAG 
GTGTTCATTG TTCAGGATCT TGAGATTCGA GATCGTTTGG CAACATCACA AATGAATAAA 
TTTTTATACC TGTATTGCAG TAAAGAAATG CCTCGAAAAG CTCACTCCAA CATGTTGACA 
GTGAAAGCCT TACACGTGTG TCCAGAATCT GGCAGGTCCC CACAGGAGTG CTGCTTGAGA 
GTGTCGCTGA TGCCGCTCCG CCTCAATATT GACCAGGATG CTTTGTTCTT CCTGAAGGAT 
TTCTTCACAA GTCTTTCTGC AGAAGTAGAG CTTCAAATGA CTCCAGATCC AGAAGTTAAA 
AAGTCTCCTG GAGCTGATGT CACCTGCAGT TTGCCAAGGC ATTTGAGTAC CTCAAAGGAG 
CCAAATCTGG TTATTTCTTT CTCTGGGCCA AAACAGCCTT CCCAAAATGA TAGTGCCAAT 
TCAGTGGAAG TGGTTAATGG CATGGAAGAG AAGAACTTCT CTGCTGAAGA AGCATCTTTT 
AGGGATCAGC CTGTGTTTTT TAGAGAATTT AGATTCACGT CAGAAGTTCC CATTCGACTT 
GATTATCATG GCAAACATGT ATCAATGGAT CAGGGTACGC TAGCTGGGAT TTTGATTGGT 
CTGGCTCAGT TAAACTGCTC TGAACTAAAG CTCAAGAGGC TTTCCTATCG ACATGGTTTA 
CTAGGCGTTG ACAAATTATT CTCATATGCA ATCACTGAGT GGCTTAATGA CATTAAGAAG 
AACCAGCTAC CAGGAATCCT GGGAGGTGTT GGACCTATGC ATTCACTAGT ACAATTAGTA 
CAAGGCCTAA AGGACTTGGT CTGGCTCCCA ATAGAGCAGT ACCGGAAGGA TGGCCGCATT 
GTCAGAGGGT TTCAGAGAGG CGCTGCTTCC TTTGGTACCT CGACAGCGAT GGCTGCTCTA 
GAACTCACAA ACAGAATGGT TCAAACCATA CAGGCAGCTG CAGAGACTGC TTATGATATG 
GTGTCTCCTG GTACCCTTTC TATCGAGCCC AAGAAGACCA AAAGGTTTCC TCATCACCGG 
TTAGCCCACC AGCCAGTAGA CCTGAGGGAA GGTGTGGCCA AGGCCTACAG TGTTGTGAAA 
GAGGGAATCA CAGACACGGC TCAGACCATT TATGAAACTG CGGCTCGAGA ACACGAGAGC 
AGAGGGGTGA CTGGTGCCGT GGGCGAGGTT CTGCGCCAGA TTCCTCCGGC AGTGGTGAAA 
CCTCTGATTG TTGCCACAGA AGCAACGTCA AACGTGCTGG GTGGCATGAG AAACCAAATT 
AGGCCAGATG TCCGGCAAGA CGAGTCACAG AAATGGCGCC ACGGGGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006720250.1
CDS	366..6599
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVRGLEMVFRPRPRPATGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTVYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTAPVETEPKLSPSWNPKIIYEPHPQLTRNLPEIAPSDPVQIGRLIGRLELSLTLKQNEVLPGAKLDVDGQIDSIHLLLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSVGVSSEQSFYETETARTPSSREEVFFSMADMDMSHSLSSLPPLGDPPNMDLELSLTSTYTNTPAGSPLSATVLQPTWGEFLDHHKEQPVRGSTFPSNLVHPTPLQKTSLPSRSVSVDESRPELIFRLAVGTFSISVLHIDPLSPPETSQNLNPLTPMAVAFFTCIEKIDPARFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMEFLECLFPTDFHSVPPHYTELLTFHSKEETGSHSPVCLQLHYKHSENRGPQGNQARLSSVPHKAELQIKLNPVCCELDISIVDRLNSLLQPQKLATVEMMASHMYTSYNKHISLHKAFTEVFLDDSHSPANCRISVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILYLAFTDLEFKTEFIGGSTPEQIKLELTFRELIGSFQEEKGDPSIKFFHVSSGVDGDTTSSDDFDWPRIVLKINPPAMHSILERIAAEEEEENDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQDKAISNSHYVLELTLPNIYVTLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFNKDSFSAFKSAVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLNINHGLIAVFTDVKQDNGDLLENKHGEFWLEFNSGSLFCVTKYEGFDDKHYICLHSSSFSLYHKGIVNGVILPTETRLPSSTRPHWLEPTIYSSEEDGLSKTSSDGVGGDSLNMLSVAVKILSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLSRDYVRVMDMGLLELTITAVKSDSDGEQTEPRFELHCSSDVVHIRTCSDSCAALMNLIQYIASYGDLQTPNKADMKPGAFQRRSKVDSSGRSSSRGPVLPEADQQMLRDLMSDAMEEIDMQQGTSSVKPQANGVLDEKSQIQEPCCSDLFLFPDESGNVSQESGPTYASFSHHFISDAMTGVPTENDDFCILFAPKAAMQEKEEEPVIKIMVDDAIVIRDNYFSLPVNKTDTSKAPLHFPIPVIRYVVKEVSLVWHLYGGKDFGIVPPTSPAKSYISPHSSPSHTPTRHGRNTVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPDCDSSLSEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTVKALHVCPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSAEVELQMTPDPEVKKSPGADVTCSLPRHLSTSKEPNLVISFSGPKQPSQNDSANSVEVVNGMEEKNFSAEEASFRDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLSYRHGLLGVDKLFSYAITEWLNDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKTKRFPHHRLAHQPVDLREGVAKAYSVVKEGITDTAQTIYETAAREHESRGVTGAVGEVLRQIPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGDD

Target ORF information:

RefSeq Version	XM_006720187.2
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy related 2B (ATG2B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006720187.2

ORF Insert Sequence:

ATGCCTTGGC CGTTTTCGGA GTCCATCAAG AAGAGGGCCT GCCGGTACCT CCTGCAGAGG 
TACCTGGGCC ACTTTCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAAGGCACCG GGTCCCTCGC CCAGGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCTTG 
GAGTCAGCAG ATGCACCCTT AGAAGTCACT GAAGGATTCA TTCAGTCAAT TTCCCTGTCA 
GTTCCATGGG GCTCTTTACT GCAGGATAAT TGTGCACTGG AAGTGAGAGG ATTAGAAATG 
GTCTTCCGGC CTAGACCTCG CCCAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTT 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAACTAAC AGATGAACAA 
GGAGAAGGAT CCCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAAAAGTCAC TTTTATAGAT ACTGTTTTGA GAATTGAACA TGTGCCAGAA 
AATTCCAAAA CTGGAACTGC ACTTGAAATT CGAATAGAAA GAACTGTGTA CTGTGATGAA 
ACTGCTGACG AATCCTCAGG AATTAATGTG CATCAACCCA CTGCTTTTGC TCACAAGTTA 
CTTCAGCTCT CTGGAGTGTC TCTCTTCTGG GATGAGTTTT CTGCATCAGC CAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TCTCACCTAG CTGGAACCCC 
AAAATTATTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGAT AGCACCTTCT 
GACCCAGTGC AGATTGGACG GTTAATTGGT AGGTTGGAGT TGAGTCTCAC GTTGAAACAG 
AATGAAGTGC TTCCTGGAGC TAAGTTGGAT GTTGATGGAC AGATAGACTC TATTCATCTA 
CTCCTGTCAC CAAGACAGGT GCACTTGCTT TTGGATATGT TGGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGGA AAAATCGACC CATGCAGCAG 
GAAGACGAGT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTC 
TCTGTGGGTG TATCTTCAGA GCAAAGCTTT TATGAGACAG AAACAGCTCG TACACCTTCT 
AGCCGTGAAG AAGTATTCTT CTCCATGGCT GATATGGACA TGTCTCATAG TCTCTCTTCT 
CTTCCACCCC TTGGGGACCC CCCAAACATG GACCTTGAGT TATCATTAAC TAGTACATAT 
ACAAATACCC CAGCAGGATC TCCATTAAGT GCTACTGTGC TTCAGCCCAC TTGGGGAGAG 
TTCCTTGATC ATCATAAAGA ACAGCCAGTA AGAGGGTCAA CATTTCCATC CAACCTAGTT 
CACCCAACAC CTTTACAGAA GACATCTCTC CCATCTAGAT CTGTTTCAGT GGATGAATCA 
AGGCCTGAAC TTATTTTTAG ACTAGCTGTG GGAACTTTTT CAATCTCTGT GCTTCACATT 
GATCCTTTAT CTCCACCTGA AACGTCACAG AACCTTAATC CATTGACACC TATGGCAGTA 
GCTTTCTTTA CTTGTATAGA AAAGATTGAT CCAGCAAGAT TTTCAACAGA AGATTTTAAG 
TCTTTCCGAG CAGTGTTTGC AGAAGCTTGC TCACACGATC ACCTTAGATT TATAGGTACT 
GGCATTAAAG TATCCTATGA ACAAAGACAA AGATCAGCTT CCCGATATTT TAGTACTGAT 
ATGTCCATTG GGCAAATGGA ATTTTTGGAG TGCCTATTTC CAACTGATTT TCATTCTGTT 
CCTCCTCACT ATACAGAGCT TTTAACGTTC CATTCCAAAG AAGAAACTGG TTCCCATTCC 
CCTGTGTGTC TTCAGCTTCA TTATAAGCAT TCTGAGAATA GAGGGCCCCA GGGTAATCAA 
GCAAGACTTA GTTCAGTTCC TCACAAGGCA GAATTGCAAA TTAAATTAAA TCCAGTGTGT 
TGTGAGCTGG ATATCAGTAT TGTGGACAGG TTAAATTCCT TGCTTCAACC ACAGAAACTT 
GCCACAGTAG AGATGATGGC ATCCCACATG TATACTTCAT ATAATAAACA TATTAGTCTG 
CACAAGGCTT TCACTGAAGT GTTTCTAGAT GATTCACATA GTCCTGCAAA TTGTCGGATA 
TCAGTACAAG TTGCCACACC AGCATTAAAC CTTTCTGTTC GCTTCCCAAT ACCTGATCTT 
CGATCTGATC AAGAAAGAGG ACCATGGTTT AAGAAGTCAC TTCAGAAGGA GATCCTTTAT 
TTAGCCTTCA CAGATCTAGA ATTTAAGACT GAATTTATAG GAGGATCAAC CCCAGAACAA 
ATTAAATTGG AACTTACCTT TAGAGAACTA ATTGGATCGT TCCAGGAAGA GAAAGGAGAT 
CCATCTATTA AGTTTTTCCA TGTGTCTAGT GGAGTAGATG GAGATACAAC ATCGTCAGAT 
GACTTTGACT GGCCACGAAT TGTACTGAAA ATAAATCCAC CAGCCATGCA TTCCATTTTG 
GAGAGAATTG CAGCTGAAGA AGAAGAGGAG AATGATGGTC ACTACCAGGA GGAAGAGGAA 
GGAGGTGCTC ATTCCTTGAA AGATGTTTGT GATCTAAGAA GACCAGCCCC ATCTCCTTTT 
TCTTCTCGTA GAGTAATGTT TGAAAATGAA CAGATGGTGA TGCCAGGAGA CCCTGTAGAA 
ATGACAGAAT TTCAGGATAA AGCAATCAGC AATTCTCACT ATGTGCTGGA ACTTACGTTA 
CCAAATATTT ATGTAACACT ACCTAATAAG AGCTTTTATG AGAAGCTTTA TAATAGGATC 
TTTAATGACT TGCTACTGTG GGAACCAACA GCTCCTTCAC CAGTGGAGAC ATTCGAGAAT 
ATTTCCTATG GCATTGGGCT TTCAGTAGCC AGTCAGCTCA TTAATACTTT CAACAAAGAT 
AGTTTTAGTG CATTTAAATC TGCAGTTCAC TATGATGAGG AAAGTGGATC TGAGGAGGAG 
ACTTTGCAGT ATTTTTCCAC TGTTGATCCC AACTATCGTT CTCGCAGGAA AAAAAAATTA 
GACTCTCAGA ACAAGAACTC TCAGAGTTTT CTCTCAGTTC TTCTGAATAT TAATCATGGA 
TTAATAGCAG TGTTCACAGA TGTGAAGCAA GATAATGGAG ATCTGTTGGA AAACAAGCAT 
GGTGAATTCT GGTTAGAGTT CAATAGTGGT TCATTATTTT GTGTGACAAA ATATGAAGGT 
TTTGATGACA AGCACTACAT TTGCCTTCAT TCTAGCAGTT TCAGTCTGTA CCATAAAGGC 
ATAGTGAATG GAGTGATTCT CCCGACAGAA ACACGACTTC CCAGCTCAAC CCGCCCACAC 
TGGTTGGAAC CTACTATTTA TTCCTCTGAA GAAGATGGCC TCAGTAAAAC TTCTTCAGAT 
GGAGTTGGAG GAGACAGTTT GAATATGCTG TCTGTTGCCG TTAAAATATT GTCTGATAAA 
TCAGAGTCCA ATACAAAGGA ATTTCTCATT GCCGTAGGAC TGAAAGGAGC CACTCTCCAG 
CATAGAATGC TTCCTTCTGG GCTTAGCTGG CATGAGCAGA TTTTATACTT CTTGAATATT 
GCTGATGAAC CTGTTTTGGG ATATAATCCT CCAACTTCAT TTACAACTTT TCATGTTCAT 
CTTTGGAGCT GTGCACTTGA TTATAGACCC CTTTATTTGC CAATCCGATC TCTTCTTACC 
GTGGAAACAT TCAGTGTTTC CAGTAGCGTT GCATTGGATA AATCTTCCTC TACTCTCAGA 
ATAATCTTGG ATGAAGCTGC TTTACATCTA TCTGACAAAT GCAATACTGT CACTATAAAT 
CTGAGTAGAG ATTATGTTCG TGTGATGGAT ATGGGGCTTT TGGAGTTAAC CATAACTGCA 
GTGAAGTCTG ATTCTGATGG AGAGCAAACT GAGCCCCGCT TTGAGTTACA CTGTTCCAGC 
GATGTTGTCC ATATCAGAAC GTGCTCAGAC TCTTGTGCTG CGTTAATGAA TCTCATTCAG 
TACATTGCAA GCTATGGTGA CTTGCAGACA CCTAACAAGG CAGATATGAA GCCTGGAGCC 
TTTCAAAGAA GGTCTAAGGT AGATTCCAGT GGTCGATCAT CCTCACGTGG TCCAGTACTT 
CCTGAAGCAG ATCAACAAAT GTTACGAGAT CTGATGAGTG ATGCTATGGA GGAGATCGAC 
ATGCAACAAG GCACCTCGTC AGTAAAACCA CAGGCTAATG GTGTTTTGGA TGAAAAATCT 
CAAATTCAGG AGCCATGTTG TTCAGACCTC TTCCTGTTTC CTGACGAGAG TGGGAATGTA 
TCCCAGGAGT CCGGCCCCAC CTATGCCTCA TTCTCTCACC ATTTCATCAG TGATGCAATG 
ACAGGTGTGC CCACTGAGAA TGATGACTTT TGCATTCTTT TTGCACCAAA AGCAGCCATG 
CAGGAGAAGG AAGAAGAACC AGTTATAAAA ATCATGGTTG ATGATGCAAT TGTGATAAGA 
GACAATTATT TCAGTCTGCC CGTTAATAAG ACCGATACGA GCAAAGCCCC CTTACACTTT 
CCCATTCCTG TGATTCGCTA TGTGGTGAAG GAGGTCTCTC TTGTCTGGCA TCTTTATGGA 
GGAAAGGATT TTGGAATAGT CCCTCCCACT TCTCCGGCTA AAAGTTATAT TAGTCCCCAC 
AGTTCGCCTT CTCACACACC CACGAGACAT GGACGTAATA CAGTATGTGG GGGAAAAGGA 
AGGAACCATG ACTTTTTAAT GGAAATACAG CTAAGCAAGG TGAAGTTTCA GCATGAAGTC 
TACCCGCCAT GCAAACCTGA TTGTGATTCC AGCCTCTCAG AACACCCAGT CTCCCGGCAG 
GTGTTCATTG TTCAGGATCT TGAGATTCGA GATCGTTTGG CAACATCACA AATGAATAAA 
TTTTTATACC TGTATTGCAG TAAAGAAATG CCTCGAAAAG CTCACTCCAA CATGTTGACA 
GTGAAAGCCT TACACGTGTG TCCAGAATCT GGCAGGTCCC CACAGGAGTG CTGCTTGAGA 
GTGTCGCTGA TGCCGCTCCG CCTCAATATT GACCAGGATG CTTTGTTCTT CCTGAAGGAT 
TTCTTCACAA GTCTTTCTGC AGAAGTAGAG CTTCAAATGA CTCCAGATCC AGAAGTTAAA 
AAGTCTCCTG GAGCTGATGT CACCTGCAGT TTGCCAAGGC ATTTGAGTAC CTCAAAGGAG 
CCAAATCTGG TTATTTCTTT CTCTGGGCCA AAACAGCCTT CCCAAAATGA TAGTGCCAAT 
TCAGTGGAAG TGGTTAATGG CATGGAAGAG AAGAACTTCT CTGCTGAAGA AGCATCTTTT 
AGGGATCAGC CTGTGTTTTT TAGAGAATTT AGATTCACGT CAGAAGTTCC CATTCGACTT 
GATTATCATG GCAAACATGT ATCAATGGAT CAGGGTACGC TAGCTGGGAT TTTGATTGGT 
CTGGCTCAGT TAAACTGCTC TGAACTAAAG CTCAAGAGGC TTTCCTATCG ACATGGTTTA 
CTAGGCGTTG ACAAATTATT CTCATATGCA ATCACTGAGT GGCTTAATGA CATTAAGAAG 
AACCAGCTAC CAGGAATCCT GGGAGGTGTT GGACCTATGC ATTCACTAGT ACAATTAGTA 
CAAGGCCTAA AGGACTTGGT CTGGCTCCCA ATAGAGCAGT ACCGGAAGGA TGGCCGCATT 
GTCAGAGGGT TTCAGAGAGG CGCTGCTTCC TTTGGTACCT CGACAGCGAT GGCTGCTCTA 
GAACTCACAA ACAGAATGGT TCAAACCATA CAGGCAGCTG CAGAGACTGC TTATGATATG 
GTGTCTCCTG GTACCCTTTC TATCGAGCCC AAGAAGACCA AAAGGTTTCC TCATCACCGG 
TTAGCCCACC AGCCAGTAGA CCTGAGGGAA GGTGTGGCCA AGGCCTACAG TGTTGTGAAA 
GAGGGAATCA CAGACACGGC TCAGACCATT TATGAAACTG CGGCTCGAGA ACACGAGAGC 
AGAGGGGTGA CTGGTGCCGT GGGCGAGGTT CTGCGCCAGA TTCCTCCGGC AGTGGTGAAA 
CCTCTGATTG TTGCCACAGA AGCAACGTCA AACGTGCTGG GTGGCATGAG AAACCAAATT 
AGGCCAGATG TCCGGCAAGA CGAGTCACAG AAATGGCGCC ACGGGGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Atg2A/B deficiency switches cytoprotective autophagy to non-canonical caspase-8 activation and apoptosis.
Cell death and differentiation24(12)2127-2138(2017 Dec)
Tang Z,Takahashi Y,Chen C,Liu Y,He H,Tsotakos N,Serfass JM,Gebru MT,Chen H,Young MM,Wang HG

Investigation of Association between Autophagy-Related Gene Polymorphisms and Pseudoexfoliation Syndrome and Pseudoexfoliation Glaucoma in a Spanish Population.
Seminars in ophthalmology1-6(2016 Dec)
de Juan-Marcos L,Escudero-Dom?nguez FA,Hern?ndez-Galilea E,Cruz-Gonz?lez F,Follana-Neira I,Gonz?lez-Sarmiento R

Aberrant methylation of ATG2B, ATG4D, ATG9A and ATG9B CpG island promoter is associated with decreased mRNA expression in sporadic breast carcinoma.
Gene590(2)285-92(2016 Sep)
Zhang X,Li C,Wang D,Chen Q,Li CL,Li HJ

Germline duplication of ATG2B and GSKIP predisposes to familial myeloid malignancies.
Nature genetics47(10)1131-40(2015 Oct)
Saliba J,Saint-Martin C,Di Stefano A,Lenglet G,Marty C,Keren B,Pasquier F,Valle VD,Secardin L,Leroy G,Mahfoudhi E,Grosjean S,Droin N,Diop M,Dessen P,Charrier S,Palazzo A,Merlevede J,Meniane JC,Delaunay-Darivon C,Fuseau P,Isnard F,Casadevall N,Solary E,Debili N,Bernard OA,Raslova H,Najman A,Vainchenker W,Bellann?-Chantelot C,Plo I

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ATG2B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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