GPATCH1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GPATCH1
Entrez Gene ID	55094
Full Name	G-patch domain containing 1
Synonyms	ECGP,GPATC1
General protein information	Preferred Names G-patch domain containing 1 Names G patch domain-containing protein 1 evolutionarily conserved G patch domain containing evolutionarily conserved G-patch domain-containing protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.11

mRNA and Protein(s)

mRNA	Protein	Name
XM_006723255.3	XP_006723318.1	G patch domain-containing protein 1 isoform X1
NM_018025.2	NP_060495.2	G patch domain-containing protein 1
NM_018025.3	NP_060495.2	G patch domain-containing protein 1
XM_006723255.4	XP_006723318.1	G patch domain-containing protein 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GPATCH1	XP_512571.3
	GPATCH1	XP_001107610.1
Bos taurus (cattle)	GPATCH1	NP_001069246.1
Mus musculus (house mouse)	Gpatch1	NP_080457.1
Homo sapiens (human)	GPATCH1	NP_060495.2
Rattus norvegicus (Norway rat)	Gpatch1	NP_001099716.1
Danio rerio (zebrafish)	gpatch1	NP_001191052.1
Canis lupus familiaris (dog)	GPATCH1	XP_853864.2
Gallus gallus (chicken)	GPATCH1	XP_004944287.1
Xenopus tropicalis (tropical clawed frog)	gpatch1	NP_001116191.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of Toll-like receptor 2 interaction with Ecgp96 controls Escherichia coli K1 invasion of brain endothelial cells.
Krishnan S, Chen S, Turcatel G, Arditi M, Prasadarao NV
Cellular microbiology15(1)63-81(2013 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In vitro, OmpA+ Escherichia coli infection selectively upregulates Ecgp96 and TLR2 in human brain microvascular endothelial cells (HBMEC), whereas OmpA- E. coli upregulates TLR4 in these cells.
Title: Regulation of Toll-like receptor 2 interaction with Ecgp96 controls Escherichia coli K1 invasion of brain endothelial cells.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

The following GPATCH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GPATCH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu29265	NM_018025.3 Latest version!	Homo sapiens G-patch domain containing 1 (GPATCH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu52335	XM_006723255.4 Latest version!	Homo sapiens G-patch domain containing 1 (GPATCH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
View Old Accession Versions >>
OHu29265	NM_018025.2	Homo sapiens G-patch domain containing 1 (GPATCH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu52335	XM_006723255.3	Homo sapiens G-patch domain containing 1 (GPATCH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GPATCH1 ( NM_018025.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_060495.2 Homo sapiens G-patch domain containing 1 (GPATCH1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu29265
Clone ID Related Accession (Same CDS sequence)	NM_018025.2 , NM_018025.3
Accession Version	NM_018025.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	G patch domain-containing protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC006108.1. On Jun 10, 2002 this sequence version replaced NM_018025.1. Transcript Variant: This variant (1) is the protein-coding transcript. ##Evidence-Data-START## Transcript exon combination :: BC006108.1, SRR1803612.275437.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGC GGGACAGTGA CAGCGAAGAA GATCTGGTCA GCTATGGGAC CGGGCTGGAG 
CCTCTGGAAG AAGGTGAAAG ACCAAAGAAA CCAATCCCTC TTCAGGATCA GACTGTCAGA 
GATGAAAAAG GAAGGTATAA ACGATTCCAC GGGGCCTTTA GTGGAGGTTT CTCTGCTGGA 
TACTTCAATA CTGTTGGCTC AAAAGAAGGA TGGACACCCT CTACCTTTGT GTCTTCACGA 
CAGAACAGAG CAGACAAATC TGTTCTTGGT CCTGAAGATT TTATGGATGA AGAGGATCTT 
AGTGAATTTG GGATAGCACC TAAAGCGATT GTCACCACAG ACGATTTTGC CTCCAAAACC 
AAAGACAGAA TACGAGAAAA GGCTAGGCAG TTGGCCGCTG CTACTGCCCC TATTCCTGGA 
GCCACCCTCC TTGATGACCT CATAACGCCA GCAAAATTAT CTGTTGGTTT CGAATTGCTA 
AGAAAAATGG GTTGGAAAGA AGGACAAGGA GTTGGTCCTC GAGTAAAGAG ACGGCCACGC 
CGACAGAAAC CTGATCCTGG AGTCAAAATC TATGGCTGTG CATTACCCCC TGGAAGCTCG 
GAAGGATCTG AGGGTGAAGA TGATGACTAC TTGCCTGATA ATGTGACCTT TGCACCCAAA 
GATGTCACAC CTGTGGATTT CACACCTAAA GATAATGTGC ATGGTCTAGC TTACAAGGGC 
CTGGATCCCC ACCAGGCACT GTTTGGAACT TCGGGAGAAC ATTTTAATCT TTTCAGTGGT 
GGTTCTGAGA GAGCTGGCGA TCTTGGAGAA ATTGGACTGA ATAAAGGAAG AAAATTGGGA 
ATTTCAGGCC AGGCTTTTGG TGTAGGTGCC CTGGAAGAGG AAGATGATGA TATCTATGCC 
ACAGAAACTC TATCCAAGTA TGACACTGTT CTGAAGGACG AGGAGCCTGG AGACGGACTC 
TATGGCTGGA CAGCACCCAG GCAGTATAAA AACCAGAAAG AATCAGAGAA AGACCTTCGG 
TACGTTGGCA AAATTTTGGA TGGATTTTCC TTGGCTTCTA AACCTTTATC TTCTAAGAAA 
ATCTATCCAC CTCCAGAGCT GCCAAGAGAC TATCGACCAG TGCATTATTT CAGACCCATG 
GTGGCCGCCA CCTCCGAGAA CTCACACTTA CTGCAGGTAT TATCAGAGTC AGCTGGAAAG 
GCAACGCCTG ACCCAGGGAC ACACAGTAAG CACCAACTGA ATGCCTCCAA ACGGGCTGAG 
TTGCTTGGAG AGACGCCTAT TCAAGGTTCA GCTACTTCAG TGTTAGAATT TCTGTCCCAA 
AAAGACAAAG AGAGAATCAA AGAAATGAAG CAGGCAACTG ACCTGAAAGC AGCTCAGCTC 
AAGGCCAGGA GTCTGGCCCA GAACGCTCAG AGCAGCAGAG CCCAGCTCTC CCCTGCAGCG 
GCTGCTGGGC ACTGCTCTTG GAACATGGCA TTAGGTGGTG GGACGGCCAC CTTAAAAGCC 
AGCAACTTCA AGCCTTTCGC CAAAGATCCG GAAAAGCAAA AGCGATACGA CGAGTTCTTA 
GTACACATGA AACAGGGTCA GAAAGATGCT CTGGAACGCT GTCTGGACCC CAGCATGACA 
GAGTGGGAGC GAGGCCGTGA GCGGGATGAG TTTGCCCGGG CGGCCCTGCT GTACGCATCT 
TCCCATTCGA CCTTGTCCTC CAGGTTCACT CACGCCAAGG AGGAGGATGA CTCAGATCAG 
GTTGAAGTCC CTCGAGACCA AGAGAATGAT GTCGGGGATA AGCAGTCGGC TGTGAAGATG 
AAGATGTTTG GGAAGCTCAC CCGAGACACG TTTGAGTGGC ACCCTGACAA GCTTCTATGT 
AAGAGATTTA ATGTCCCTGA CCCTTATCCA GATTCAACTT TAGTTGGCTT ACCAAGAGTG 
AAGCGTGACA AGTACTCAGT CTTCAACTTT CTGACGCTCC CAGAGACAGC TTCCTTGCCC 
ACCACTCAAG CATCAAGTGA AAAAGTATCA CAGCACCGAG GTCCCGACAA ATCAAGAAAA 
CCATCCAGAT GGGATACCTC TAAACACGAA AAGAAAGAAG ATTCCATTAG TGAATTTTTA 
AGTTTGGCTA GATCAAAAGC CGAGCCACCT AAACAACAGT CCAGCCCCTT AGTAAACAAA 
GAGGAAGAGC ATGCACCAGA ATTATCCGCA AATCAGACCG TCAACAAAGA TGTGGACGCA 
CAGGCTGAAG GAGAAGGGAG CCGCCCATCC ATGGACTTAT TCAGGGCCAT CTTTGCCAGT 
TCCTCAGATG AAAAGTCCTC ATCCTCCGAG GATGAGCAAG GTGACAGTGA AGATGATCAG 
GCAGGCTCTG GGGAGGCCAA CTTCCAAAGC TCCCAAGACA CTGACTTGGG GGAAACATCA 
TCTGTGGCTC ACGCTCTTGT GCCAGCACCC CAGGAGCCGC CACCTTCCTT CCCGATACAA 
AAGATGCAGA TAGATGAAAG AGAAGAGTTC GGCCCGCGGC TGCCTCCCGT CTTCTGCCCC 
AATGCTCGTC AGACACTTGA GGTTCCTCAA AAAGAGAAAC ATAAAAAGAA CAAAGACAAG 
CACAAGGCCA AGAAAGAGCA CAGGCGGAAG AAAGAGAAGA AAAAGAAACA CAGGAAGCAC 
AAACACAAAG GCAAGCAAAA GAATAAAAAA CCAGAGAAAA GTAGTAGCTC CGAGAGTTCC 
GACAGCAGCG ACAGCCAGAG TGACGAGGAA ACCGCAGACG TGTCGCCCCA GGAGCTGCTG 
AGACGGCTGA AAAGTCTTCC ACTAAGAAGG CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060495.2
CDS	315..3110
Translation	MAARDSDSEEDLVSYGTGLEPLEEGERPKKPIPLQDQTVRDEKGRYKRFHGAFSGGFSAGYFNTVGSKEGWTPSTFVSSRQNRADKSVLGPEDFMDEEDLSEFGIAPKAIVTTDDFASKTKDRIREKARQLAAATAPIPGATLLDDLITPAKLSVGFELLRKMGWKEGQGVGPRVKRRPRRQKPDPGVKIYGCALPPGSSEGSEGEDDDYLPDNVTFAPKDVTPVDFTPKDNVHGLAYKGLDPHQALFGTSGEHFNLFSGGSERAGDLGEIGLNKGRKLGISGQAFGVGALEEEDDDIYATETLSKYDTVLKDEEPGDGLYGWTAPRQYKNQKESEKDLRYVGKILDGFSLASKPLSSKKIYPPPELPRDYRPVHYFRPMVAATSENSHLLQVLSESAGKATPDPGTHSKHQLNASKRAELLGETPIQGSATSVLEFLSQKDKERIKEMKQATDLKAAQLKARSLAQNAQSSRAQLSPAAAAGHCSWNMALGGGTATLKASNFKPFAKDPEKQKRYDEFLVHMKQGQKDALERCLDPSMTEWERGRERDEFARAALLYASSHSTLSSRFTHAKEEDDSDQVEVPRDQENDVGDKQSAVKMKMFGKLTRDTFEWHPDKLLCKRFNVPDPYPDSTLVGLPRVKRDKYSVFNFLTLPETASLPTTQASSEKVSQHRGPDKSRKPSRWDTSKHEKKEDSISEFLSLARSKAEPPKQQSSPLVNKEEEHAPELSANQTVNKDVDAQAEGEGSRPSMDLFRAIFASSSDEKSSSSEDEQGDSEDDQAGSGEANFQSSQDTDLGETSSVAHALVPAPQEPPPSFPIQKMQIDEREEFGPRLPPVFCPNARQTLEVPQKEKHKKNKDKHKAKKEHRRKKEKKKKHRKHKHKGKQKNKKPEKSSSSESSDSSDSQSDEETADVSPQELLRRLKSLPLRRQ

Target ORF information:

RefSeq Version	NM_018025.2
Organism	Homo sapiens(human)
Definition	Homo sapiens G-patch domain containing 1 (GPATCH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018025.2

ORF Insert Sequence:

ATGGCGGCGC GGGACAGTGA CAGCGAAGAA GATCTGGTCA GCTATGGGAC CGGGCTGGAG 
CCTCTGGAAG AAGGTGAAAG ACCAAAGAAA CCAATCCCTC TTCAGGATCA GACTGTCAGA 
GATGAAAAAG GAAGGTATAA ACGATTCCAC GGGGCCTTTA GTGGAGGTTT CTCTGCTGGA 
TACTTCAATA CTGTTGGCTC AAAAGAAGGA TGGACACCCT CTACCTTTGT GTCTTCACGA 
CAGAACAGAG CAGACAAATC TGTTCTTGGT CCTGAAGATT TTATGGATGA AGAGGATCTT 
AGTGAATTTG GGATAGCACC TAAAGCGATT GTCACCACAG ACGATTTTGC CTCCAAAACC 
AAAGACAGAA TACGAGAAAA GGCTAGGCAG TTGGCCGCTG CTACTGCCCC TATTCCTGGA 
GCCACCCTCC TTGATGACCT CATAACGCCA GCAAAATTAT CTGTTGGTTT CGAATTGCTA 
AGAAAAATGG GTTGGAAAGA AGGACAAGGA GTTGGTCCTC GAGTAAAGAG ACGGCCACGC 
CGACAGAAAC CTGATCCTGG AGTCAAAATC TATGGCTGTG CATTACCCCC TGGAAGCTCG 
GAAGGATCTG AGGGTGAAGA TGATGACTAC TTGCCTGATA ATGTGACCTT TGCACCCAAA 
GATGTCACAC CTGTGGATTT CACACCTAAA GATAATGTGC ATGGTCTAGC TTACAAGGGC 
CTGGATCCCC ACCAGGCACT GTTTGGAACT TCGGGAGAAC ATTTTAATCT TTTCAGTGGT 
GGTTCTGAGA GAGCTGGCGA TCTTGGAGAA ATTGGACTGA ATAAAGGAAG AAAATTGGGA 
ATTTCAGGCC AGGCTTTTGG TGTAGGTGCC CTGGAAGAGG AAGATGATGA TATCTATGCC 
ACAGAAACTC TATCCAAGTA TGACACTGTT CTGAAGGACG AGGAGCCTGG AGACGGACTC 
TATGGCTGGA CAGCACCCAG GCAGTATAAA AACCAGAAAG AATCAGAGAA AGACCTTCGG 
TACGTTGGCA AAATTTTGGA TGGATTTTCC TTGGCTTCTA AACCTTTATC TTCTAAGAAA 
ATCTATCCAC CTCCAGAGCT GCCAAGAGAC TATCGACCAG TGCATTATTT CAGACCCATG 
GTGGCCGCCA CCTCCGAGAA CTCACACTTA CTGCAGGTAT TATCAGAGTC AGCTGGAAAG 
GCAACGCCTG ACCCAGGGAC ACACAGTAAG CACCAACTGA ATGCCTCCAA ACGGGCTGAG 
TTGCTTGGAG AGACGCCTAT TCAAGGTTCA GCTACTTCAG TGTTAGAATT TCTGTCCCAA 
AAAGACAAAG AGAGAATCAA AGAAATGAAG CAGGCAACTG ACCTGAAAGC AGCTCAGCTC 
AAGGCCAGGA GTCTGGCCCA GAACGCTCAG AGCAGCAGAG CCCAGCTCTC CCCTGCAGCG 
GCTGCTGGGC ACTGCTCTTG GAACATGGCA TTAGGTGGTG GGACGGCCAC CTTAAAAGCC 
AGCAACTTCA AGCCTTTCGC CAAAGATCCG GAAAAGCAAA AGCGATACGA CGAGTTCTTA 
GTACACATGA AACAGGGTCA GAAAGATGCT CTGGAACGCT GTCTGGACCC CAGCATGACA 
GAGTGGGAGC GAGGCCGTGA GCGGGATGAG TTTGCCCGGG CGGCCCTGCT GTACGCATCT 
TCCCATTCGA CCTTGTCCTC CAGGTTCACT CACGCCAAGG AGGAGGATGA CTCAGATCAG 
GTTGAAGTCC CTCGAGACCA AGAGAATGAT GTCGGGGATA AGCAGTCGGC TGTGAAGATG 
AAGATGTTTG GGAAGCTCAC CCGAGACACG TTTGAGTGGC ACCCTGACAA GCTTCTATGT 
AAGAGATTTA ATGTCCCTGA CCCTTATCCA GATTCAACTT TAGTTGGCTT ACCAAGAGTG 
AAGCGTGACA AGTACTCAGT CTTCAACTTT CTGACGCTCC CAGAGACAGC TTCCTTGCCC 
ACCACTCAAG CATCAAGTGA AAAAGTATCA CAGCACCGAG GTCCCGACAA ATCAAGAAAA 
CCATCCAGAT GGGATACCTC TAAACACGAA AAGAAAGAAG ATTCCATTAG TGAATTTTTA 
AGTTTGGCTA GATCAAAAGC CGAGCCACCT AAACAACAGT CCAGCCCCTT AGTAAACAAA 
GAGGAAGAGC ATGCACCAGA ATTATCCGCA AATCAGACCG TCAACAAAGA TGTGGACGCA 
CAGGCTGAAG GAGAAGGGAG CCGCCCATCC ATGGACTTAT TCAGGGCCAT CTTTGCCAGT 
TCCTCAGATG AAAAGTCCTC ATCCTCCGAG GATGAGCAAG GTGACAGTGA AGATGATCAG 
GCAGGCTCTG GGGAGGCCAA CTTCCAAAGC TCCCAAGACA CTGACTTGGG GGAAACATCA 
TCTGTGGCTC ACGCTCTTGT GCCAGCACCC CAGGAGCCGC CACCTTCCTT CCCGATACAA 
AAGATGCAGA TAGATGAAAG AGAAGAGTTC GGCCCGCGGC TGCCTCCCGT CTTCTGCCCC 
AATGCTCGTC AGACACTTGA GGTTCCTCAA AAAGAGAAAC ATAAAAAGAA CAAAGACAAG 
CACAAGGCCA AGAAAGAGCA CAGGCGGAAG AAAGAGAAGA AAAAGAAACA CAGGAAGCAC 
AAACACAAAG GCAAGCAAAA GAATAAAAAA CCAGAGAAAA GTAGTAGCTC CGAGAGTTCC 
GACAGCAGCG ACAGCCAGAG TGACGAGGAA ACCGCAGACG TGTCGCCCCA GGAGCTGCTG 
AGACGGCTGA AAAGTCTTCC ACTAAGAAGG CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPATCH1 ( NM_018025.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060495.2 Homo sapiens G-patch domain containing 1 (GPATCH1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu29265
Clone ID Related Accession (Same CDS sequence)	NM_018025.2 , NM_018025.3
Accession Version	NM_018025.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	G patch domain-containing protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC006108.1 and AC008521.5. On May 17, 2019 this sequence version replaced NM_018025.2. Transcript Variant: This variant (1) is the protein-coding transcript. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.51100.1, SRR1803613.199913.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000170564.7/ ENSP00000170564.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGC GGGACAGTGA CAGCGAAGAA GATCTGGTCA GCTATGGGAC CGGGCTGGAG 
CCTCTGGAAG AAGGTGAAAG ACCAAAGAAA CCAATCCCTC TTCAGGATCA GACTGTCAGA 
GATGAAAAAG GAAGGTATAA ACGATTCCAC GGGGCCTTTA GTGGAGGTTT CTCTGCTGGA 
TACTTCAATA CTGTTGGCTC AAAAGAAGGA TGGACACCCT CTACCTTTGT GTCTTCACGA 
CAGAACAGAG CAGACAAATC TGTTCTTGGT CCTGAAGATT TTATGGATGA AGAGGATCTT 
AGTGAATTTG GGATAGCACC TAAAGCGATT GTCACCACAG ACGATTTTGC CTCCAAAACC 
AAAGACAGAA TACGAGAAAA GGCTAGGCAG TTGGCCGCTG CTACTGCCCC TATTCCTGGA 
GCCACCCTCC TTGATGACCT CATAACGCCA GCAAAATTAT CTGTTGGTTT CGAATTGCTA 
AGAAAAATGG GTTGGAAAGA AGGACAAGGA GTTGGTCCTC GAGTAAAGAG ACGGCCACGC 
CGACAGAAAC CTGATCCTGG AGTCAAAATC TATGGCTGTG CATTACCCCC TGGAAGCTCG 
GAAGGATCTG AGGGTGAAGA TGATGACTAC TTGCCTGATA ATGTGACCTT TGCACCCAAA 
GATGTCACAC CTGTGGATTT CACACCTAAA GATAATGTGC ATGGTCTAGC TTACAAGGGC 
CTGGATCCCC ACCAGGCACT GTTTGGAACT TCGGGAGAAC ATTTTAATCT TTTCAGTGGT 
GGTTCTGAGA GAGCTGGCGA TCTTGGAGAA ATTGGACTGA ATAAAGGAAG AAAATTGGGA 
ATTTCAGGCC AGGCTTTTGG TGTAGGTGCC CTGGAAGAGG AAGATGATGA TATCTATGCC 
ACAGAAACTC TATCCAAGTA TGACACTGTT CTGAAGGACG AGGAGCCTGG AGACGGACTC 
TATGGCTGGA CAGCACCCAG GCAGTATAAA AACCAGAAAG AATCAGAGAA AGACCTTCGG 
TACGTTGGCA AAATTTTGGA TGGATTTTCC TTGGCTTCTA AACCTTTATC TTCTAAGAAA 
ATCTATCCAC CTCCAGAGCT GCCAAGAGAC TATCGACCAG TGCATTATTT CAGACCCATG 
GTGGCCGCCA CCTCCGAGAA CTCACACTTA CTGCAGGTAT TATCAGAGTC AGCTGGAAAG 
GCAACGCCTG ACCCAGGGAC ACACAGTAAG CACCAACTGA ATGCCTCCAA ACGGGCTGAG 
TTGCTTGGAG AGACGCCTAT TCAAGGTTCA GCTACTTCAG TGTTAGAATT TCTGTCCCAA 
AAAGACAAAG AGAGAATCAA AGAAATGAAG CAGGCAACTG ACCTGAAAGC AGCTCAGCTC 
AAGGCCAGGA GTCTGGCCCA GAACGCTCAG AGCAGCAGAG CCCAGCTCTC CCCTGCAGCG 
GCTGCTGGGC ACTGCTCTTG GAACATGGCA TTAGGTGGTG GGACGGCCAC CTTAAAAGCC 
AGCAACTTCA AGCCTTTCGC CAAAGATCCG GAAAAGCAAA AGCGATACGA CGAGTTCTTA 
GTACACATGA AACAGGGTCA GAAAGATGCT CTGGAACGCT GTCTGGACCC CAGCATGACA 
GAGTGGGAGC GAGGCCGTGA GCGGGATGAG TTTGCCCGGG CGGCCCTGCT GTACGCATCT 
TCCCATTCGA CCTTGTCCTC CAGGTTCACT CACGCCAAGG AGGAGGATGA CTCAGATCAG 
GTTGAAGTCC CTCGAGACCA AGAGAATGAT GTCGGGGATA AGCAGTCGGC TGTGAAGATG 
AAGATGTTTG GGAAGCTCAC CCGAGACACG TTTGAGTGGC ACCCTGACAA GCTTCTATGT 
AAGAGATTTA ATGTCCCTGA CCCTTATCCA GATTCAACTT TAGTTGGCTT ACCAAGAGTG 
AAGCGTGACA AGTACTCAGT CTTCAACTTT CTGACGCTCC CAGAGACAGC TTCCTTGCCC 
ACCACTCAAG CATCAAGTGA AAAAGTATCA CAGCACCGAG GTCCCGACAA ATCAAGAAAA 
CCATCCAGAT GGGATACCTC TAAACACGAA AAGAAAGAAG ATTCCATTAG TGAATTTTTA 
AGTTTGGCTA GATCAAAAGC CGAGCCACCT AAACAACAGT CCAGCCCCTT AGTAAACAAA 
GAGGAAGAGC ATGCACCAGA ATTATCCGCA AATCAGACCG TCAACAAAGA TGTGGACGCA 
CAGGCTGAAG GAGAAGGGAG CCGCCCATCC ATGGACTTAT TCAGGGCCAT CTTTGCCAGT 
TCCTCAGATG AAAAGTCCTC ATCCTCCGAG GATGAGCAAG GTGACAGTGA AGATGATCAG 
GCAGGCTCTG GGGAGGCCAA CTTCCAAAGC TCCCAAGACA CTGACTTGGG GGAAACATCA 
TCTGTGGCTC ACGCTCTTGT GCCAGCACCC CAGGAGCCGC CACCTTCCTT CCCGATACAA 
AAGATGCAGA TAGATGAAAG AGAAGAGTTC GGCCCGCGGC TGCCTCCCGT CTTCTGCCCC 
AATGCTCGTC AGACACTTGA GGTTCCTCAA AAAGAGAAAC ATAAAAAGAA CAAAGACAAG 
CACAAGGCCA AGAAAGAGCA CAGGCGGAAG AAAGAGAAGA AAAAGAAACA CAGGAAGCAC 
AAACACAAAG GCAAGCAAAA GAATAAAAAA CCAGAGAAAA GTAGTAGCTC CGAGAGTTCC 
GACAGCAGCG ACAGCCAGAG TGACGAGGAA ACCGCAGACG TGTCGCCCCA GGAGCTGCTG 
AGACGGCTGA AAAGTCTTCC ACTAAGAAGG CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060495.2
CDS	14..2809
Translation	MAARDSDSEEDLVSYGTGLEPLEEGERPKKPIPLQDQTVRDEKGRYKRFHGAFSGGFSAGYFNTVGSKEGWTPSTFVSSRQNRADKSVLGPEDFMDEEDLSEFGIAPKAIVTTDDFASKTKDRIREKARQLAAATAPIPGATLLDDLITPAKLSVGFELLRKMGWKEGQGVGPRVKRRPRRQKPDPGVKIYGCALPPGSSEGSEGEDDDYLPDNVTFAPKDVTPVDFTPKDNVHGLAYKGLDPHQALFGTSGEHFNLFSGGSERAGDLGEIGLNKGRKLGISGQAFGVGALEEEDDDIYATETLSKYDTVLKDEEPGDGLYGWTAPRQYKNQKESEKDLRYVGKILDGFSLASKPLSSKKIYPPPELPRDYRPVHYFRPMVAATSENSHLLQVLSESAGKATPDPGTHSKHQLNASKRAELLGETPIQGSATSVLEFLSQKDKERIKEMKQATDLKAAQLKARSLAQNAQSSRAQLSPAAAAGHCSWNMALGGGTATLKASNFKPFAKDPEKQKRYDEFLVHMKQGQKDALERCLDPSMTEWERGRERDEFARAALLYASSHSTLSSRFTHAKEEDDSDQVEVPRDQENDVGDKQSAVKMKMFGKLTRDTFEWHPDKLLCKRFNVPDPYPDSTLVGLPRVKRDKYSVFNFLTLPETASLPTTQASSEKVSQHRGPDKSRKPSRWDTSKHEKKEDSISEFLSLARSKAEPPKQQSSPLVNKEEEHAPELSANQTVNKDVDAQAEGEGSRPSMDLFRAIFASSSDEKSSSSEDEQGDSEDDQAGSGEANFQSSQDTDLGETSSVAHALVPAPQEPPPSFPIQKMQIDEREEFGPRLPPVFCPNARQTLEVPQKEKHKKNKDKHKAKKEHRRKKEKKKKHRKHKHKGKQKNKKPEKSSSSESSDSSDSQSDEETADVSPQELLRRLKSLPLRRQ

Target ORF information:

RefSeq Version	NM_018025.3
Organism	Homo sapiens(human)
Definition	Homo sapiens G-patch domain containing 1 (GPATCH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018025.3

ORF Insert Sequence:

ATGGCGGCGC GGGACAGTGA CAGCGAAGAA GATCTGGTCA GCTATGGGAC CGGGCTGGAG 
CCTCTGGAAG AAGGTGAAAG ACCAAAGAAA CCAATCCCTC TTCAGGATCA GACTGTCAGA 
GATGAAAAAG GAAGGTATAA ACGATTCCAC GGGGCCTTTA GTGGAGGTTT CTCTGCTGGA 
TACTTCAATA CTGTTGGCTC AAAAGAAGGA TGGACACCCT CTACCTTTGT GTCTTCACGA 
CAGAACAGAG CAGACAAATC TGTTCTTGGT CCTGAAGATT TTATGGATGA AGAGGATCTT 
AGTGAATTTG GGATAGCACC TAAAGCGATT GTCACCACAG ACGATTTTGC CTCCAAAACC 
AAAGACAGAA TACGAGAAAA GGCTAGGCAG TTGGCCGCTG CTACTGCCCC TATTCCTGGA 
GCCACCCTCC TTGATGACCT CATAACGCCA GCAAAATTAT CTGTTGGTTT CGAATTGCTA 
AGAAAAATGG GTTGGAAAGA AGGACAAGGA GTTGGTCCTC GAGTAAAGAG ACGGCCACGC 
CGACAGAAAC CTGATCCTGG AGTCAAAATC TATGGCTGTG CATTACCCCC TGGAAGCTCG 
GAAGGATCTG AGGGTGAAGA TGATGACTAC TTGCCTGATA ATGTGACCTT TGCACCCAAA 
GATGTCACAC CTGTGGATTT CACACCTAAA GATAATGTGC ATGGTCTAGC TTACAAGGGC 
CTGGATCCCC ACCAGGCACT GTTTGGAACT TCGGGAGAAC ATTTTAATCT TTTCAGTGGT 
GGTTCTGAGA GAGCTGGCGA TCTTGGAGAA ATTGGACTGA ATAAAGGAAG AAAATTGGGA 
ATTTCAGGCC AGGCTTTTGG TGTAGGTGCC CTGGAAGAGG AAGATGATGA TATCTATGCC 
ACAGAAACTC TATCCAAGTA TGACACTGTT CTGAAGGACG AGGAGCCTGG AGACGGACTC 
TATGGCTGGA CAGCACCCAG GCAGTATAAA AACCAGAAAG AATCAGAGAA AGACCTTCGG 
TACGTTGGCA AAATTTTGGA TGGATTTTCC TTGGCTTCTA AACCTTTATC TTCTAAGAAA 
ATCTATCCAC CTCCAGAGCT GCCAAGAGAC TATCGACCAG TGCATTATTT CAGACCCATG 
GTGGCCGCCA CCTCCGAGAA CTCACACTTA CTGCAGGTAT TATCAGAGTC AGCTGGAAAG 
GCAACGCCTG ACCCAGGGAC ACACAGTAAG CACCAACTGA ATGCCTCCAA ACGGGCTGAG 
TTGCTTGGAG AGACGCCTAT TCAAGGTTCA GCTACTTCAG TGTTAGAATT TCTGTCCCAA 
AAAGACAAAG AGAGAATCAA AGAAATGAAG CAGGCAACTG ACCTGAAAGC AGCTCAGCTC 
AAGGCCAGGA GTCTGGCCCA GAACGCTCAG AGCAGCAGAG CCCAGCTCTC CCCTGCAGCG 
GCTGCTGGGC ACTGCTCTTG GAACATGGCA TTAGGTGGTG GGACGGCCAC CTTAAAAGCC 
AGCAACTTCA AGCCTTTCGC CAAAGATCCG GAAAAGCAAA AGCGATACGA CGAGTTCTTA 
GTACACATGA AACAGGGTCA GAAAGATGCT CTGGAACGCT GTCTGGACCC CAGCATGACA 
GAGTGGGAGC GAGGCCGTGA GCGGGATGAG TTTGCCCGGG CGGCCCTGCT GTACGCATCT 
TCCCATTCGA CCTTGTCCTC CAGGTTCACT CACGCCAAGG AGGAGGATGA CTCAGATCAG 
GTTGAAGTCC CTCGAGACCA AGAGAATGAT GTCGGGGATA AGCAGTCGGC TGTGAAGATG 
AAGATGTTTG GGAAGCTCAC CCGAGACACG TTTGAGTGGC ACCCTGACAA GCTTCTATGT 
AAGAGATTTA ATGTCCCTGA CCCTTATCCA GATTCAACTT TAGTTGGCTT ACCAAGAGTG 
AAGCGTGACA AGTACTCAGT CTTCAACTTT CTGACGCTCC CAGAGACAGC TTCCTTGCCC 
ACCACTCAAG CATCAAGTGA AAAAGTATCA CAGCACCGAG GTCCCGACAA ATCAAGAAAA 
CCATCCAGAT GGGATACCTC TAAACACGAA AAGAAAGAAG ATTCCATTAG TGAATTTTTA 
AGTTTGGCTA GATCAAAAGC CGAGCCACCT AAACAACAGT CCAGCCCCTT AGTAAACAAA 
GAGGAAGAGC ATGCACCAGA ATTATCCGCA AATCAGACCG TCAACAAAGA TGTGGACGCA 
CAGGCTGAAG GAGAAGGGAG CCGCCCATCC ATGGACTTAT TCAGGGCCAT CTTTGCCAGT 
TCCTCAGATG AAAAGTCCTC ATCCTCCGAG GATGAGCAAG GTGACAGTGA AGATGATCAG 
GCAGGCTCTG GGGAGGCCAA CTTCCAAAGC TCCCAAGACA CTGACTTGGG GGAAACATCA 
TCTGTGGCTC ACGCTCTTGT GCCAGCACCC CAGGAGCCGC CACCTTCCTT CCCGATACAA 
AAGATGCAGA TAGATGAAAG AGAAGAGTTC GGCCCGCGGC TGCCTCCCGT CTTCTGCCCC 
AATGCTCGTC AGACACTTGA GGTTCCTCAA AAAGAGAAAC ATAAAAAGAA CAAAGACAAG 
CACAAGGCCA AGAAAGAGCA CAGGCGGAAG AAAGAGAAGA AAAAGAAACA CAGGAAGCAC 
AAACACAAAG GCAAGCAAAA GAATAAAAAA CCAGAGAAAA GTAGTAGCTC CGAGAGTTCC 
GACAGCAGCG ACAGCCAGAG TGACGAGGAA ACCGCAGACG TGTCGCCCCA GGAGCTGCTG 
AGACGGCTGA AAAGTCTTCC ACTAAGAAGG CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPATCH1 ( XM_006723255.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006723318.1 Homo sapiens G-patch domain containing 1 (GPATCH1), transcript variant X1, mRNA.

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CloneID	OHu52335
Clone ID Related Accession (Same CDS sequence)	XM_006723255.3 , XM_006723255.4
Accession Version	XM_006723255.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	G patch domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006723255.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC GGGACAGTGA CAGCGAAGAA GATCTGGTCA GCTATGGGAC CGGGCTGGAG 
CCTCTGGAAG AAGGTGAAAG ACCAAAGAAA CCAATCCCTC TTCAGGATCA GACTGTCAGA 
GATGAAAAAG GAAGGTATAA ACGATTCCAC GGGGCCTTTA GTGGAGGTTT CTCTGCTGGA 
TACTTCAATA CTGTTGGCTC AAAAGAAGGA TGGACACCCT CTACCTTTGT GTCTTCACGA 
CAGAACAGAG CAGACAAATC TGTTCTTGGT CCTGAAGATT TTATGGATGA AGAGGATCTT 
AGTGAATTTG GGATAGCACC TAAAGCGATT GTCACCACAG ACGATTTTGC CTCCAAAACC 
AAAGACAGAA TACGAGAAAA GGCTAGGCAG TTGGCCGCTG CTACTGCCCC TATTCCTGGA 
GCCACCCTCC TTGATGACCT CATAACGCCA GCAAAATTAT CTGTTGGTTT CGAATTGCTA 
AGAAAAATGG GTTGGAAAGA AGGACAAGGA GTTGGTCCTC GAGTAAAGAG ACGGCCACGC 
CGACAGAAAC CTGATCCTGG AGTCAAAATC TATGGCTGTG CATTACCCCC TGGAAGCTCG 
GAAGGATCTG AGGGTGAAGA TGATGACTAC TTGCCTGATA ATGTGACCTT TGCACCCAAA 
GATGTCACAC CTGTGGATTT CACACCTAAA GATAATGTGC ATGGTCTAGC TTACAAGGGC 
CTGGATCCCC ACCAGGCACT GTTTGGAACT TCGGGAGAAC ATTTTAATCT TTTCAGTGGT 
GGTTCTGAGA GAGCTGGCGA TCTTGGAGAA ATTGGACTGA ATAAAGGAAG AAAATTGGGA 
ATTTCAGGCC AGGCTTTTGG TGTAGGTGCC CTGGAAGAGG AAGATGATGA TATCTATGCC 
ACAGAAACTC TATCCAAGTA TGACACTGTT CTGAAGGACG AGGAGCCTGG AGACGGACTC 
TATGGCTGGA CAGCACCCAG GCAGTATAAA AACCAGAAAG AATCAGAGAA AGACCTTCGG 
TACGTTGGCA AAATTTTGGA TGGATTTTCC TTGGCTTCTA AACCTTTATC TTCTAAGAAA 
ATCTATCCAC CTCCAGAGCT GCCAAGAGAC TATCGACCAG TGCATTATTT CAGACCCATG 
GTGGCCGCCA CCTCCGAGAA CTCACACTTA CTGCAGGTAT TATCAGAGTC AGCTGGAAAG 
GCAACGCCTG ACCCAGGGAC ACACAGTAAG CACCAACTGA ATGCCTCCAA ACGGGCTGAG 
TTGCTTGGAG AGACGCCTAT TCAAGGTTCA GCTACTTCAG TGTTAGAATT TCTGTCCCAA 
AAAGACAAAG AGAGAATCAA AGAAATGAAG CAGGCAACTG ACCTGAAAGC AGCTCAGCTC 
AAGGCCAGGA GTCTGGCCCA GAACGCTCAG AGCAGCAGAG CCCAGCTCTC CCCTGCAGCG 
GCTGCTGGGC ACTGCTCTTG GAACATGGCA TTAGGTGGTG GGACGGCCAC CTTAAAAGCC 
AGCAACTTCA AGCCTTTCGC CAAAGATCCG GAAAAGCAAA AGCGATACGA CGAGTTCTTA 
GTACACATGA AACAGGGTCA GAAAGATGCT CTGGAACGCT GTCTGGACCC CAGCATGACA 
GAGTGGGAGC GAGGCCGTGA GCGGGATGAG TTTGCCCGGG CGGCCCTGCT GTACGCATCT 
TCCCATTCGA CCTTGTCCTC CAGGTTCACT CACGCCAAGG AGGAGGATGA CTCAGATCAG 
GTTGAAGTCC CTCGAGACCA AGAGAATGAT GTCGGGGATA AGCAGTCGGC TGTGAAGATG 
AAGATGTTTG GGAAGCTCAC CCGAGACACG TTTGAGTGGC ACCCTGACAA GCTTCTATTT 
CAACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006723318.1
CDS	30..1898
Translation	MAARDSDSEEDLVSYGTGLEPLEEGERPKKPIPLQDQTVRDEKGRYKRFHGAFSGGFSAGYFNTVGSKEGWTPSTFVSSRQNRADKSVLGPEDFMDEEDLSEFGIAPKAIVTTDDFASKTKDRIREKARQLAAATAPIPGATLLDDLITPAKLSVGFELLRKMGWKEGQGVGPRVKRRPRRQKPDPGVKIYGCALPPGSSEGSEGEDDDYLPDNVTFAPKDVTPVDFTPKDNVHGLAYKGLDPHQALFGTSGEHFNLFSGGSERAGDLGEIGLNKGRKLGISGQAFGVGALEEEDDDIYATETLSKYDTVLKDEEPGDGLYGWTAPRQYKNQKESEKDLRYVGKILDGFSLASKPLSSKKIYPPPELPRDYRPVHYFRPMVAATSENSHLLQVLSESAGKATPDPGTHSKHQLNASKRAELLGETPIQGSATSVLEFLSQKDKERIKEMKQATDLKAAQLKARSLAQNAQSSRAQLSPAAAAGHCSWNMALGGGTATLKASNFKPFAKDPEKQKRYDEFLVHMKQGQKDALERCLDPSMTEWERGRERDEFARAALLYASSHSTLSSRFTHAKEEDDSDQVEVPRDQENDVGDKQSAVKMKMFGKLTRDTFEWHPDKLLFQL

Target ORF information:

RefSeq Version	XM_006723255.3
Organism	Homo sapiens(human)
Definition	Homo sapiens G-patch domain containing 1 (GPATCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006723255.3

ORF Insert Sequence:

ATGGCGGCGC GGGACAGTGA CAGCGAAGAA GATCTGGTCA GCTATGGGAC CGGGCTGGAG 
CCTCTGGAAG AAGGTGAAAG ACCAAAGAAA CCAATCCCTC TTCAGGATCA GACTGTCAGA 
GATGAAAAAG GAAGGTATAA ACGATTCCAC GGGGCCTTTA GTGGAGGTTT CTCTGCTGGA 
TACTTCAATA CTGTTGGCTC AAAAGAAGGA TGGACACCCT CTACCTTTGT GTCTTCACGA 
CAGAACAGAG CAGACAAATC TGTTCTTGGT CCTGAAGATT TTATGGATGA AGAGGATCTT 
AGTGAATTTG GGATAGCACC TAAAGCGATT GTCACCACAG ACGATTTTGC CTCCAAAACC 
AAAGACAGAA TACGAGAAAA GGCTAGGCAG TTGGCCGCTG CTACTGCCCC TATTCCTGGA 
GCCACCCTCC TTGATGACCT CATAACGCCA GCAAAATTAT CTGTTGGTTT CGAATTGCTA 
AGAAAAATGG GTTGGAAAGA AGGACAAGGA GTTGGTCCTC GAGTAAAGAG ACGGCCACGC 
CGACAGAAAC CTGATCCTGG AGTCAAAATC TATGGCTGTG CATTACCCCC TGGAAGCTCG 
GAAGGATCTG AGGGTGAAGA TGATGACTAC TTGCCTGATA ATGTGACCTT TGCACCCAAA 
GATGTCACAC CTGTGGATTT CACACCTAAA GATAATGTGC ATGGTCTAGC TTACAAGGGC 
CTGGATCCCC ACCAGGCACT GTTTGGAACT TCGGGAGAAC ATTTTAATCT TTTCAGTGGT 
GGTTCTGAGA GAGCTGGCGA TCTTGGAGAA ATTGGACTGA ATAAAGGAAG AAAATTGGGA 
ATTTCAGGCC AGGCTTTTGG TGTAGGTGCC CTGGAAGAGG AAGATGATGA TATCTATGCC 
ACAGAAACTC TATCCAAGTA TGACACTGTT CTGAAGGACG AGGAGCCTGG AGACGGACTC 
TATGGCTGGA CAGCACCCAG GCAGTATAAA AACCAGAAAG AATCAGAGAA AGACCTTCGG 
TACGTTGGCA AAATTTTGGA TGGATTTTCC TTGGCTTCTA AACCTTTATC TTCTAAGAAA 
ATCTATCCAC CTCCAGAGCT GCCAAGAGAC TATCGACCAG TGCATTATTT CAGACCCATG 
GTGGCCGCCA CCTCCGAGAA CTCACACTTA CTGCAGGTAT TATCAGAGTC AGCTGGAAAG 
GCAACGCCTG ACCCAGGGAC ACACAGTAAG CACCAACTGA ATGCCTCCAA ACGGGCTGAG 
TTGCTTGGAG AGACGCCTAT TCAAGGTTCA GCTACTTCAG TGTTAGAATT TCTGTCCCAA 
AAAGACAAAG AGAGAATCAA AGAAATGAAG CAGGCAACTG ACCTGAAAGC AGCTCAGCTC 
AAGGCCAGGA GTCTGGCCCA GAACGCTCAG AGCAGCAGAG CCCAGCTCTC CCCTGCAGCG 
GCTGCTGGGC ACTGCTCTTG GAACATGGCA TTAGGTGGTG GGACGGCCAC CTTAAAAGCC 
AGCAACTTCA AGCCTTTCGC CAAAGATCCG GAAAAGCAAA AGCGATACGA CGAGTTCTTA 
GTACACATGA AACAGGGTCA GAAAGATGCT CTGGAACGCT GTCTGGACCC CAGCATGACA 
GAGTGGGAGC GAGGCCGTGA GCGGGATGAG TTTGCCCGGG CGGCCCTGCT GTACGCATCT 
TCCCATTCGA CCTTGTCCTC CAGGTTCACT CACGCCAAGG AGGAGGATGA CTCAGATCAG 
GTTGAAGTCC CTCGAGACCA AGAGAATGAT GTCGGGGATA AGCAGTCGGC TGTGAAGATG 
AAGATGTTTG GGAAGCTCAC CCGAGACACG TTTGAGTGGC ACCCTGACAA GCTTCTATTT 
CAACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPATCH1 ( XM_006723255.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006723318.1 Homo sapiens G-patch domain containing 1 (GPATCH1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu52335
Clone ID Related Accession (Same CDS sequence)	XM_006723255.3 , XM_006723255.4
Accession Version	XM_006723255.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	G patch domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006723255.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC GGGACAGTGA CAGCGAAGAA GATCTGGTCA GCTATGGGAC CGGGCTGGAG 
CCTCTGGAAG AAGGTGAAAG ACCAAAGAAA CCAATCCCTC TTCAGGATCA GACTGTCAGA 
GATGAAAAAG GAAGGTATAA ACGATTCCAC GGGGCCTTTA GTGGAGGTTT CTCTGCTGGA 
TACTTCAATA CTGTTGGCTC AAAAGAAGGA TGGACACCCT CTACCTTTGT GTCTTCACGA 
CAGAACAGAG CAGACAAATC TGTTCTTGGT CCTGAAGATT TTATGGATGA AGAGGATCTT 
AGTGAATTTG GGATAGCACC TAAAGCGATT GTCACCACAG ACGATTTTGC CTCCAAAACC 
AAAGACAGAA TACGAGAAAA GGCTAGGCAG TTGGCCGCTG CTACTGCCCC TATTCCTGGA 
GCCACCCTCC TTGATGACCT CATAACGCCA GCAAAATTAT CTGTTGGTTT CGAATTGCTA 
AGAAAAATGG GTTGGAAAGA AGGACAAGGA GTTGGTCCTC GAGTAAAGAG ACGGCCACGC 
CGACAGAAAC CTGATCCTGG AGTCAAAATC TATGGCTGTG CATTACCCCC TGGAAGCTCG 
GAAGGATCTG AGGGTGAAGA TGATGACTAC TTGCCTGATA ATGTGACCTT TGCACCCAAA 
GATGTCACAC CTGTGGATTT CACACCTAAA GATAATGTGC ATGGTCTAGC TTACAAGGGC 
CTGGATCCCC ACCAGGCACT GTTTGGAACT TCGGGAGAAC ATTTTAATCT TTTCAGTGGT 
GGTTCTGAGA GAGCTGGCGA TCTTGGAGAA ATTGGACTGA ATAAAGGAAG AAAATTGGGA 
ATTTCAGGCC AGGCTTTTGG TGTAGGTGCC CTGGAAGAGG AAGATGATGA TATCTATGCC 
ACAGAAACTC TATCCAAGTA TGACACTGTT CTGAAGGACG AGGAGCCTGG AGACGGACTC 
TATGGCTGGA CAGCACCCAG GCAGTATAAA AACCAGAAAG AATCAGAGAA AGACCTTCGG 
TACGTTGGCA AAATTTTGGA TGGATTTTCC TTGGCTTCTA AACCTTTATC TTCTAAGAAA 
ATCTATCCAC CTCCAGAGCT GCCAAGAGAC TATCGACCAG TGCATTATTT CAGACCCATG 
GTGGCCGCCA CCTCCGAGAA CTCACACTTA CTGCAGGTAT TATCAGAGTC AGCTGGAAAG 
GCAACGCCTG ACCCAGGGAC ACACAGTAAG CACCAACTGA ATGCCTCCAA ACGGGCTGAG 
TTGCTTGGAG AGACGCCTAT TCAAGGTTCA GCTACTTCAG TGTTAGAATT TCTGTCCCAA 
AAAGACAAAG AGAGAATCAA AGAAATGAAG CAGGCAACTG ACCTGAAAGC AGCTCAGCTC 
AAGGCCAGGA GTCTGGCCCA GAACGCTCAG AGCAGCAGAG CCCAGCTCTC CCCTGCAGCG 
GCTGCTGGGC ACTGCTCTTG GAACATGGCA TTAGGTGGTG GGACGGCCAC CTTAAAAGCC 
AGCAACTTCA AGCCTTTCGC CAAAGATCCG GAAAAGCAAA AGCGATACGA CGAGTTCTTA 
GTACACATGA AACAGGGTCA GAAAGATGCT CTGGAACGCT GTCTGGACCC CAGCATGACA 
GAGTGGGAGC GAGGCCGTGA GCGGGATGAG TTTGCCCGGG CGGCCCTGCT GTACGCATCT 
TCCCATTCGA CCTTGTCCTC CAGGTTCACT CACGCCAAGG AGGAGGATGA CTCAGATCAG 
GTTGAAGTCC CTCGAGACCA AGAGAATGAT GTCGGGGATA AGCAGTCGGC TGTGAAGATG 
AAGATGTTTG GGAAGCTCAC CCGAGACACG TTTGAGTGGC ACCCTGACAA GCTTCTATTT 
CAACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006723318.1
CDS	27..1895
Translation	MAARDSDSEEDLVSYGTGLEPLEEGERPKKPIPLQDQTVRDEKGRYKRFHGAFSGGFSAGYFNTVGSKEGWTPSTFVSSRQNRADKSVLGPEDFMDEEDLSEFGIAPKAIVTTDDFASKTKDRIREKARQLAAATAPIPGATLLDDLITPAKLSVGFELLRKMGWKEGQGVGPRVKRRPRRQKPDPGVKIYGCALPPGSSEGSEGEDDDYLPDNVTFAPKDVTPVDFTPKDNVHGLAYKGLDPHQALFGTSGEHFNLFSGGSERAGDLGEIGLNKGRKLGISGQAFGVGALEEEDDDIYATETLSKYDTVLKDEEPGDGLYGWTAPRQYKNQKESEKDLRYVGKILDGFSLASKPLSSKKIYPPPELPRDYRPVHYFRPMVAATSENSHLLQVLSESAGKATPDPGTHSKHQLNASKRAELLGETPIQGSATSVLEFLSQKDKERIKEMKQATDLKAAQLKARSLAQNAQSSRAQLSPAAAAGHCSWNMALGGGTATLKASNFKPFAKDPEKQKRYDEFLVHMKQGQKDALERCLDPSMTEWERGRERDEFARAALLYASSHSTLSSRFTHAKEEDDSDQVEVPRDQENDVGDKQSAVKMKMFGKLTRDTFEWHPDKLLFQL

Target ORF information:

RefSeq Version	XM_006723255.4
Organism	Homo sapiens(human)
Definition	Homo sapiens G-patch domain containing 1 (GPATCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006723255.4

ORF Insert Sequence:

ATGGCGGCGC GGGACAGTGA CAGCGAAGAA GATCTGGTCA GCTATGGGAC CGGGCTGGAG 
CCTCTGGAAG AAGGTGAAAG ACCAAAGAAA CCAATCCCTC TTCAGGATCA GACTGTCAGA 
GATGAAAAAG GAAGGTATAA ACGATTCCAC GGGGCCTTTA GTGGAGGTTT CTCTGCTGGA 
TACTTCAATA CTGTTGGCTC AAAAGAAGGA TGGACACCCT CTACCTTTGT GTCTTCACGA 
CAGAACAGAG CAGACAAATC TGTTCTTGGT CCTGAAGATT TTATGGATGA AGAGGATCTT 
AGTGAATTTG GGATAGCACC TAAAGCGATT GTCACCACAG ACGATTTTGC CTCCAAAACC 
AAAGACAGAA TACGAGAAAA GGCTAGGCAG TTGGCCGCTG CTACTGCCCC TATTCCTGGA 
GCCACCCTCC TTGATGACCT CATAACGCCA GCAAAATTAT CTGTTGGTTT CGAATTGCTA 
AGAAAAATGG GTTGGAAAGA AGGACAAGGA GTTGGTCCTC GAGTAAAGAG ACGGCCACGC 
CGACAGAAAC CTGATCCTGG AGTCAAAATC TATGGCTGTG CATTACCCCC TGGAAGCTCG 
GAAGGATCTG AGGGTGAAGA TGATGACTAC TTGCCTGATA ATGTGACCTT TGCACCCAAA 
GATGTCACAC CTGTGGATTT CACACCTAAA GATAATGTGC ATGGTCTAGC TTACAAGGGC 
CTGGATCCCC ACCAGGCACT GTTTGGAACT TCGGGAGAAC ATTTTAATCT TTTCAGTGGT 
GGTTCTGAGA GAGCTGGCGA TCTTGGAGAA ATTGGACTGA ATAAAGGAAG AAAATTGGGA 
ATTTCAGGCC AGGCTTTTGG TGTAGGTGCC CTGGAAGAGG AAGATGATGA TATCTATGCC 
ACAGAAACTC TATCCAAGTA TGACACTGTT CTGAAGGACG AGGAGCCTGG AGACGGACTC 
TATGGCTGGA CAGCACCCAG GCAGTATAAA AACCAGAAAG AATCAGAGAA AGACCTTCGG 
TACGTTGGCA AAATTTTGGA TGGATTTTCC TTGGCTTCTA AACCTTTATC TTCTAAGAAA 
ATCTATCCAC CTCCAGAGCT GCCAAGAGAC TATCGACCAG TGCATTATTT CAGACCCATG 
GTGGCCGCCA CCTCCGAGAA CTCACACTTA CTGCAGGTAT TATCAGAGTC AGCTGGAAAG 
GCAACGCCTG ACCCAGGGAC ACACAGTAAG CACCAACTGA ATGCCTCCAA ACGGGCTGAG 
TTGCTTGGAG AGACGCCTAT TCAAGGTTCA GCTACTTCAG TGTTAGAATT TCTGTCCCAA 
AAAGACAAAG AGAGAATCAA AGAAATGAAG CAGGCAACTG ACCTGAAAGC AGCTCAGCTC 
AAGGCCAGGA GTCTGGCCCA GAACGCTCAG AGCAGCAGAG CCCAGCTCTC CCCTGCAGCG 
GCTGCTGGGC ACTGCTCTTG GAACATGGCA TTAGGTGGTG GGACGGCCAC CTTAAAAGCC 
AGCAACTTCA AGCCTTTCGC CAAAGATCCG GAAAAGCAAA AGCGATACGA CGAGTTCTTA 
GTACACATGA AACAGGGTCA GAAAGATGCT CTGGAACGCT GTCTGGACCC CAGCATGACA 
GAGTGGGAGC GAGGCCGTGA GCGGGATGAG TTTGCCCGGG CGGCCCTGCT GTACGCATCT 
TCCCATTCGA CCTTGTCCTC CAGGTTCACT CACGCCAAGG AGGAGGATGA CTCAGATCAG 
GTTGAAGTCC CTCGAGACCA AGAGAATGAT GTCGGGGATA AGCAGTCGGC TGTGAAGATG 
AAGATGTTTG GGAAGCTCAC CCGAGACACG TTTGAGTGGC ACCCTGACAA GCTTCTATTT 
CAACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of Toll-like receptor 2 interaction with Ecgp96 controls Escherichia coli K1 invasion of brain endothelial cells.
Cellular microbiology15(1)63-81(2013 Jan)
Krishnan S,Chen S,Turcatel G,Arditi M,Prasadarao NV

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GPATCH1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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