PPP1R3D cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPP1R3D
Entrez Gene ID	5509
Full Name	protein phosphatase 1 regulatory subunit 3D
Synonyms	PPP1R6
General protein information	Preferred Names protein phosphatase 1 regulatory subunit 3D Names protein phosphatase 1 regulatory subunit 3D PP1 subunit R6 protein phosphatase 1 regulatory subunit 6 protein phosphatase 1, regulatory (inhibitor) subunit 3D protein phosphatase 1, regulatory subunit 6, spinophilin protein phosphatase 1-binding subunit R6
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20q13.33
Summary	Phosphorylation of serine and threonine residues in proteins is a crucial step in the regulation of many cellular functions ranging from hormonal regulation to cell division and even short-term memory. The level of phosphorylation is controlled by the opposing actions of protein kinases and protein phosphatases. Protein phosphatase 1 (PP1) is 1 of 4 major serine/threonine-specific protein phosphatases which have been identified in eukaryotic cells. PP1 associates with various regulatory subunits that dictate its subcellular localization and modulate its substrate specificity. Several subunits that target PP1 to glycogen have been identified. This gene encodes a glycogen-targeting subunit of PP1. [provided by RefSeq, Jul 2008].
Disorder MIM:	603326

mRNA and Protein(s)

mRNA	Protein	Name
NM_006242.4	NP_006233.1	protein phosphatase 1 regulatory subunit 3D
NM_006242.3	NP_006233.1	protein phosphatase 1 regulatory subunit 3D

Pathways from BioSystems

Homologies

Homo sapiens (human)	PPP1R3D	NP_006233.1
Pan troglodytes (chimpanzee)	PPP1R3D	XP_001142110.2
Canis lupus familiaris (dog)	PPP1R3D	XP_005635305.1
Bos taurus (cattle)	PPP1R3D	XP_005214993.1
Danio rerio (zebrafish)	ppp1r3da	NP_001103882.2
Rattus norvegicus (Norway rat)	Ppp1r3d	NP_001103034.1
Danio rerio (zebrafish)	LOC567375	XP_003199449.1
Gallus gallus (chicken)	PPP1R3D	XP_004947134.1
Drosophila melanogaster (fruit fly)	Gbs-76A	NP_001262043.1
Xenopus tropicalis (tropical clawed frog)	ppp1r3d	XP_004919781.1
	PPP1R3D	XP_001090753.1
Mus musculus (house mouse)	Ppp1r3d	NP_001078970.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Systematic Analysis of Human Protein Phosphatase Interactions and Dynamics.
Yadav L, Tamene F, G??s H, van Drogen A, Katainen R, Aebersold R, Gstaiger M, Varjosalo M
Cell systems4(4)430-444.e5(2017 Apr)

The interaction between AMPK?2 and the PP1-targeting subunit R6 is dynamically regulated by intracellular glycogen content.
Oligschlaeger Y, Miglianico M, Dahlmans V, Rubio-Villena C, Chanda D, Garcia-Gimeno MA, Coumans WA, Liu Y, Voncken JW, Luiken JJ, Glatz JF, Sanz P, Neumann D
The Biochemical journal473(7)937-47(2016 Apr)

Structure-Function Analysis of PPP1R3D, a Protein Phosphatase 1 Targeting Subunit, Reveals a Binding Motif for 14-3-3 Proteins which Regulates its Glycogenic Properties.
Rubio-Villena C, Sanz P, Garcia-Gimeno MA
PloS one10(6)e0131476(2015)

Glycogenic activity of R6, a protein phosphatase 1 regulatory subunit, is modulated by the laforin-malin complex.
Rubio-Villena C, Garcia-Gimeno MA, Sanz P
The international journal of biochemistry & cell biology45(7)1479-88(2013 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data are in accordance with R6 binding directly to AMPKbeta2 when both proteins detach from the diminishing glycogen particle, which is simultaneous with increased AMPKbeta2 Thr-148 autophosphorylation.
Title: The interaction between AMPK?2 and the PP1-targeting subunit R6 is dynamically regulated by intracellular glycogen content.

These results define binding to 14-3-3 proteins as an additional pathway in the control of the glycogenic properties of PPP1R3D (R6).
Title: Structure-Function Analysis of PPP1R3D, a Protein Phosphatase 1 Targeting Subunit, Reveals a Binding Motif for 14-3-3 Proteins which Regulates its Glycogenic Properties.

In this work we present evidence that PPP1R3D (R6), one of the PP1 glycogenic targeting subunits, interacts physically with laforin, a glucan phosphatase involved in Lafora disease, a fatal type of progressive myoclonus epilepsy.
Title: Glycogenic activity of R6, a protein phosphatase 1 regulatory subunit, is modulated by the laforin-malin complex.

The following PPP1R3D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPP1R3D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu29193	NM_006242.4 Latest version!	Homo sapiens protein phosphatase 1 regulatory subunit 3D (PPP1R3D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu29193	NM_006242.3	Homo sapiens protein phosphatase 1 regulatory subunit 3D (PPP1R3D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPP1R3D ( NM_006242.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006233.1 Homo sapiens protein phosphatase 1 regulatory subunit 3D (PPP1R3D), mRNA.

Price & Availability↑

CloneID	OHu29193
Clone ID Related Accession (Same CDS sequence)	NM_006242.4 , NM_006242.3
Accession Version	NM_006242.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	protein phosphatase 1 regulatory subunit 3D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL109928.33. On Nov 25, 2018 this sequence version replaced NM_006242.3. ##Evidence-Data-START## Transcript is intronless :: BC047502.1, SRR1163658.532813.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000370996.5/ ENSP00000360035.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCCAGAG GCCCGAGCTC CGCGGTCCTG CCTAGCGCCC TGGGATCCCG GAAGCTCGGC 
CCCCGGAGCC TCAGCTGCCT GTCGGACCTG GACGGCGGCG TGGCCCTGGA GCCGCGGGCC 
TGTAGGCCCC CTGGGAGCCC GGGCCGCGCG CCGCCGCCAA CGCCAGCGCC GTCGGGCTGC 
GACCCCCGCC TGCGGCCCAT CATCCTGCGG CGGGCGCGCT CACTGCCCAG CTCCCCCGAG 
CGCCGCCAGA AGGCCGCGGG CGCGCCGGGC GCTGCGTGTC GGCCGGGCTG CAGCCAGAAG 
CTCCGCGTGC GCTTCGCCGA CGCCCTGGGC TTGGAGCTGG CACAGGTCAA GGTGTTCAAC 
GCGGGAGACG ACCCGTCCGT GCCGCTGCAC GTGCTGTCGC GGCTCGCAAT CAACTCGGAC 
CTGTGCTGCA GCAGCCAGGA CCTGGAGTTC ACCCTGCATT GCCTGGTGCC CGATTTCCCG 
CCGCCCGTCG AGGCCGCCGA CTTTGGCGAG CGCCTGCAGC GGCAGCTCGT GTGCCTGGAG 
CGTGTCACTT GCTCGGACCT TGGCATCAGC GGTACGGTGC GCGTGTGCAA CGTGGCCTTC 
GAGAAGCAGG TGGCTGTGCG CTACACTTTC TCGGGCTGGC GCAGTACCCA CGAGGCGGTG 
GCGCGGTGGC GCGGGCCCGC AGGCCCCGAG GGCACGGAGG ACGTTTTCAC CTTCGGCTTT 
CCAGTACCGC CCTTCCTGCT GGAGCTCGGC TCCCGCGTGC ACTTCGCGGT GCGCTACCAA 
GTGGCGGGTG CCGAGTACTG GGACAACAAC GACCACCGAG ACTACAGCCT CACATGTCGC 
AACCACGCGC TGCACATGCC TCGCGGGGAG TGCGAAGAGA GCTGGATCCA CTTCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006233.1
CDS	375..1274
Translation	MSRGPSSAVLPSALGSRKLGPRSLSCLSDLDGGVALEPRACRPPGSPGRAPPPTPAPSGCDPRLRPIILRRARSLPSSPERRQKAAGAPGAACRPGCSQKLRVRFADALGLELAQVKVFNAGDDPSVPLHVLSRLAINSDLCCSSQDLEFTLHCLVPDFPPPVEAADFGERLQRQLVCLERVTCSDLGISGTVRVCNVAFEKQVAVRYTFSGWRSTHEAVARWRGPAGPEGTEDVFTFGFPVPPFLLELGSRVHFAVRYQVAGAEYWDNNDHRDYSLTCRNHALHMPRGECEESWIHFI

Target ORF information:

RefSeq Version	NM_006242.4
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 1 regulatory subunit 3D (PPP1R3D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006242.4

ORF Insert Sequence:

ATGTCCAGAG GCCCGAGCTC CGCGGTCCTG CCTAGCGCCC TGGGATCCCG GAAGCTCGGC 
CCCCGGAGCC TCAGCTGCCT GTCGGACCTG GACGGCGGCG TGGCCCTGGA GCCGCGGGCC 
TGTAGGCCCC CTGGGAGCCC GGGCCGCGCG CCGCCGCCAA CGCCAGCGCC GTCGGGCTGC 
GACCCCCGCC TGCGGCCCAT CATCCTGCGG CGGGCGCGCT CACTGCCCAG CTCCCCCGAG 
CGCCGCCAGA AGGCCGCGGG CGCGCCGGGC GCTGCGTGTC GGCCGGGCTG CAGCCAGAAG 
CTCCGCGTGC GCTTCGCCGA CGCCCTGGGC TTGGAGCTGG CACAGGTCAA GGTGTTCAAC 
GCGGGAGACG ACCCGTCCGT GCCGCTGCAC GTGCTGTCGC GGCTCGCAAT CAACTCGGAC 
CTGTGCTGCA GCAGCCAGGA CCTGGAGTTC ACCCTGCATT GCCTGGTGCC CGATTTCCCG 
CCGCCCGTCG AGGCCGCCGA CTTTGGCGAG CGCCTGCAGC GGCAGCTCGT GTGCCTGGAG 
CGTGTCACTT GCTCGGACCT TGGCATCAGC GGTACGGTGC GCGTGTGCAA CGTGGCCTTC 
GAGAAGCAGG TGGCTGTGCG CTACACTTTC TCGGGCTGGC GCAGTACCCA CGAGGCGGTG 
GCGCGGTGGC GCGGGCCCGC AGGCCCCGAG GGCACGGAGG ACGTTTTCAC CTTCGGCTTT 
CCAGTACCGC CCTTCCTGCT GGAGCTCGGC TCCCGCGTGC ACTTCGCGGT GCGCTACCAA 
GTGGCGGGTG CCGAGTACTG GGACAACAAC GACCACCGAG ACTACAGCCT CACATGTCGC 
AACCACGCGC TGCACATGCC TCGCGGGGAG TGCGAAGAGA GCTGGATCCA CTTCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP1R3D ( NM_006242.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006233.1 Homo sapiens protein phosphatase 1 regulatory subunit 3D (PPP1R3D), mRNA.

Price & Availability↑

CloneID	OHu29193
Clone ID Related Accession (Same CDS sequence)	NM_006242.4 , NM_006242.3
Accession Version	NM_006242.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	protein phosphatase 1 regulatory subunit 3D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC047502.1. On Oct 10, 2003 this sequence version replaced NM_006242.2. ##Evidence-Data-START## Transcript is intronless :: BC047502.1 [ECO:0000345] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAGAG GCCCGAGCTC CGCGGTCCTG CCTAGCGCCC TGGGATCCCG GAAGCTCGGC 
CCCCGGAGCC TCAGCTGCCT GTCGGACCTG GACGGCGGCG TGGCCCTGGA GCCGCGGGCC 
TGTAGGCCCC CTGGGAGCCC GGGCCGCGCG CCGCCGCCAA CGCCAGCGCC GTCGGGCTGC 
GACCCCCGCC TGCGGCCCAT CATCCTGCGG CGGGCGCGCT CACTGCCCAG CTCCCCCGAG 
CGCCGCCAGA AGGCCGCGGG CGCGCCGGGC GCTGCGTGTC GGCCGGGCTG CAGCCAGAAG 
CTCCGCGTGC GCTTCGCCGA CGCCCTGGGC TTGGAGCTGG CACAGGTCAA GGTGTTCAAC 
GCGGGAGACG ACCCGTCCGT GCCGCTGCAC GTGCTGTCGC GGCTCGCAAT CAACTCGGAC 
CTGTGCTGCA GCAGCCAGGA CCTGGAGTTC ACCCTGCATT GCCTGGTGCC CGATTTCCCG 
CCGCCCGTCG AGGCCGCCGA CTTTGGCGAG CGCCTGCAGC GGCAGCTCGT GTGCCTGGAG 
CGTGTCACTT GCTCGGACCT TGGCATCAGC GGTACGGTGC GCGTGTGCAA CGTGGCCTTC 
GAGAAGCAGG TGGCTGTGCG CTACACTTTC TCGGGCTGGC GCAGTACCCA CGAGGCGGTG 
GCGCGGTGGC GCGGGCCCGC AGGCCCCGAG GGCACGGAGG ACGTTTTCAC CTTCGGCTTT 
CCAGTACCGC CCTTCCTGCT GGAGCTCGGC TCCCGCGTGC ACTTCGCGGT GCGCTACCAA 
GTGGCGGGTG CCGAGTACTG GGACAACAAC GACCACCGAG ACTACAGCCT CACATGTCGC 
AACCACGCGC TGCACATGCC TCGCGGGGAG TGCGAAGAGA GCTGGATCCA CTTCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006233.1
CDS	367..1266
Translation	MSRGPSSAVLPSALGSRKLGPRSLSCLSDLDGGVALEPRACRPPGSPGRAPPPTPAPSGCDPRLRPIILRRARSLPSSPERRQKAAGAPGAACRPGCSQKLRVRFADALGLELAQVKVFNAGDDPSVPLHVLSRLAINSDLCCSSQDLEFTLHCLVPDFPPPVEAADFGERLQRQLVCLERVTCSDLGISGTVRVCNVAFEKQVAVRYTFSGWRSTHEAVARWRGPAGPEGTEDVFTFGFPVPPFLLELGSRVHFAVRYQVAGAEYWDNNDHRDYSLTCRNHALHMPRGECEESWIHFI

Target ORF information:

RefSeq Version	NM_006242.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 1 regulatory subunit 3D (PPP1R3D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006242.3

ORF Insert Sequence:

ATGTCCAGAG GCCCGAGCTC CGCGGTCCTG CCTAGCGCCC TGGGATCCCG GAAGCTCGGC 
CCCCGGAGCC TCAGCTGCCT GTCGGACCTG GACGGCGGCG TGGCCCTGGA GCCGCGGGCC 
TGTAGGCCCC CTGGGAGCCC GGGCCGCGCG CCGCCGCCAA CGCCAGCGCC GTCGGGCTGC 
GACCCCCGCC TGCGGCCCAT CATCCTGCGG CGGGCGCGCT CACTGCCCAG CTCCCCCGAG 
CGCCGCCAGA AGGCCGCGGG CGCGCCGGGC GCTGCGTGTC GGCCGGGCTG CAGCCAGAAG 
CTCCGCGTGC GCTTCGCCGA CGCCCTGGGC TTGGAGCTGG CACAGGTCAA GGTGTTCAAC 
GCGGGAGACG ACCCGTCCGT GCCGCTGCAC GTGCTGTCGC GGCTCGCAAT CAACTCGGAC 
CTGTGCTGCA GCAGCCAGGA CCTGGAGTTC ACCCTGCATT GCCTGGTGCC CGATTTCCCG 
CCGCCCGTCG AGGCCGCCGA CTTTGGCGAG CGCCTGCAGC GGCAGCTCGT GTGCCTGGAG 
CGTGTCACTT GCTCGGACCT TGGCATCAGC GGTACGGTGC GCGTGTGCAA CGTGGCCTTC 
GAGAAGCAGG TGGCTGTGCG CTACACTTTC TCGGGCTGGC GCAGTACCCA CGAGGCGGTG 
GCGCGGTGGC GCGGGCCCGC AGGCCCCGAG GGCACGGAGG ACGTTTTCAC CTTCGGCTTT 
CCAGTACCGC CCTTCCTGCT GGAGCTCGGC TCCCGCGTGC ACTTCGCGGT GCGCTACCAA 
GTGGCGGGTG CCGAGTACTG GGACAACAAC GACCACCGAG ACTACAGCCT CACATGTCGC 
AACCACGCGC TGCACATGCC TCGCGGGGAG TGCGAAGAGA GCTGGATCCA CTTCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Systematic Analysis of Human Protein Phosphatase Interactions and Dynamics.
Cell systems4(4)430-444.e5(2017 Apr)
Yadav L,Tamene F,G??s H,van Drogen A,Katainen R,Aebersold R,Gstaiger M,Varjosalo M

The interaction between AMPK?2 and the PP1-targeting subunit R6 is dynamically regulated by intracellular glycogen content.
The Biochemical journal473(7)937-47(2016 Apr)
Oligschlaeger Y,Miglianico M,Dahlmans V,Rubio-Villena C,Chanda D,Garcia-Gimeno MA,Coumans WA,Liu Y,Voncken JW,Luiken JJ,Glatz JF,Sanz P,Neumann D

Structure-Function Analysis of PPP1R3D, a Protein Phosphatase 1 Targeting Subunit, Reveals a Binding Motif for 14-3-3 Proteins which Regulates its Glycogenic Properties.
PloS one10(6)e0131476(2015)
Rubio-Villena C,Sanz P,Garcia-Gimeno MA

Glycogenic activity of R6, a protein phosphatase 1 regulatory subunit, is modulated by the laforin-malin complex.
The international journal of biochemistry & cell biology45(7)1479-88(2013 Jul)
Rubio-Villena C,Garcia-Gimeno MA,Sanz P

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PPP1R3D cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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