RNF31 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RNF31
Entrez Gene ID	55072
Full Name	ring finger protein 31
Synonyms	HOIP,Paul,ZIBRA
General protein information	Preferred Names ring finger protein 31 Names E3 ubiquitin-protein ligase RNF31 HOIL-1-interacting protein RING-type E3 ubiquitin transferase RNF31 zinc in-between-RING-finger ubiquitin-associated domain protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q12
Summary	The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. The encoded protein is the E3 ubiquitin-protein ligase component of the linear ubiquitin chain assembly complex. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015].
Disorder MIM:	612487

mRNA and Protein(s)

mRNA	Protein	Name
NM_001310332.1	NP_001297261.1	E3 ubiquitin-protein ligase RNF31 isoform 2
NM_017999.5	NP_060469.4	E3 ubiquitin-protein ligase RNF31 isoform 1
NM_017999.4	NP_060469.4	E3 ubiquitin-protein ligase RNF31 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	RNF31	NP_060469.4
Pan troglodytes (chimpanzee)	RNF31	XP_001166671.1
Canis lupus familiaris (dog)	RNF31	XP_537383.2
Xenopus tropicalis (tropical clawed frog)	rnf31	NP_001090644.1
	RNF31	XP_001112195.1
Mus musculus (house mouse)	Rnf31	NP_919327.2
Rattus norvegicus (Norway rat)	Rnf31	NP_001102338.2
Danio rerio (zebrafish)	LOC100334103	XP_002660873.3

Gene Ontology
Bibliography

Related articles in PubMed

Structural Insights into SHARPIN-Mediated Activation of HOIP for the Linear Ubiquitin Chain Assembly.
Liu J, Wang Y, Gong Y, Fu T, Hu S, Zhou Z, Pan L
Cell reports21(1)27-36(2017 Oct)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results indicate that caspase-mediated cleavage of HOIP divides critical functional regions of HOIP, and that this regulates linear (de)ubiquitination of substrates upon apoptosis.
Title: Decreased linear ubiquitination of NEMO and FADD on apoptosis with caspase-mediated cleavage of HOIP.

Study found that RNF31 is cleaved under apoptosis conditions through various stimulations; findings elucidate a novel regulatory loop between cell death and the survival signal
Title: Regulation of Linear Ubiquitin Chain Assembly Complex by Caspase-Mediated Cleavage of RNF31.

LUBAC components control TLR3-mediated innate immunity, thereby preventing development of immunodeficiency and autoinflammation.
Title: --LUBAC deficiency perturbs TLR3 signaling to cause immunodeficiency and autoinflammation.

The data reveal SPATA2 as a high-affinity binding partner of CYLD and HOIP, and a regulatory component of linear ubiquitin chain assembly complex-mediated NF-kappaB signaling.
Title: SPATA2 Links CYLD to LUBAC, Activates CYLD, and Controls LUBAC Signaling.

SPATA2 has been described as a previously unrecognized factor in the linear ubiquitin chain assembly complex-dependent signaling pathways that serves as an adaptor between HOIP and CYLD, thereby enabling recruitment of CYLD to signaling complexes.
Title: SPATA2-Mediated Binding of CYLD to HOIP Enables CYLD Recruitment to Signaling Complexes.

The following RNF31 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNF31 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25779	NM_017999.5 Latest version!	Homo sapiens ring finger protein 31 (RNF31), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu68946	NM_001310332.1 Latest version!	Homo sapiens ring finger protein 31 (RNF31), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
View Old Accession Versions >>
OHu25779	NM_017999.4	Homo sapiens ring finger protein 31 (RNF31), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNF31 ( NM_017999.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060469.4 Homo sapiens ring finger protein 31 (RNF31), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25779
Clone ID Related Accession (Same CDS sequence)	NM_017999.5 , NM_017999.4
Accession Version	NM_017999.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF31 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN287790.1 and BC012077.1. This sequence is a reference standard in the RefSeqGene project. On Nov 20, 2018 this sequence version replaced NM_017999.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225832.1, AK291247.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000324103.11/ ENSP00000315112.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCGGGGG AGGAAGAGGA GCGGGCCTTC CTGGTGGCCC GCGAGGAGCT GGCGAGCGCC 
CTGAGGAGGG ATTCCGGGCA GGCGTTTTCC CTGGAGCAGC TCCGGCCGCT ACTAGCCAGC 
TCTCTGCCGC TAGCCGCCCG CTACCTGCAG CTGGACGCCG CACGCCTTGT CCGCTGCAAC 
GCTCATGGGG AGCCCCGAAA CTACCTCAAC ACCCTGTCCA CGGCTCTGAA CATCCTGGAG 
AAATACGGCC GCAACCTTCT CAGCCCTCAG CGGCCTCGGT ACTGGCGTGG TGTCAAGTTT 
AATAACCCTG TCTTTCGCAG CACGGTGGAT GCTGTGCAGG GGGGCCGAGA TGTGCTGCGA 
TTATATGGCT ACACAGAGGA GCAACCAGAT GGGTTGAGCT TCCCCGAAGG GCAGGAGGAG 
CCAGATGAGC ACCAGGTTGC TACAGTCACA CTGGAAGTAC TGCTGCTTCG GACAGAGCTC 
AGCCTGCTAT TGCAGAATAC TCATCCAAGA CAGCAGGCAC TGGAGCAGCT GTTGGAAGAC 
AAGGTTGAAG ATGATATGCT GCAGCTTTCA GAATTTGACC CCCTATTGAG AGAGATTGCT 
CCTGGCCCCC TCACCACACC CTCTGTCCCA GGCTCCACTC CTGGTCCCTG CTTCCTCTGT 
GGTTCTGCCC CAGGCACACT GCACTGCCCA TCCTGTAAAC AGGCCCTGTG TCCAGCCTGT 
GACCACCTGT TCCATGGACA CCCATCCCGT GCTCATCACC TCCGCCAGAC CCTGCCTGGG 
GTCCTGCAGG GTACCCACCT GAGCCCCAGT TTACCTGCCT CAGCCCAACC ACGGCCCCAG 
TCGACCTCCC TGCTGGCCCT GGGAGACAGC TCTCTTTCTT CCCCTAATCC TGCAAGTGCT 
CATTTGCCCT GGCACTGTGC TGCCTGTGCC ATGCTAAATG AGCCTTGGGC AGTGCTCTGT 
GTGGCCTGTG ATCGGCCCCG AGGCTGTAAG GGGTTGGGGT TGGGAACTGA GGGTCCCCAA 
GGAACTGGAG GCCTAGAACC TGATCTTGCA CGGGGTCGGT GGGCCTGCCA GAGCTGTACC 
TTTGAGAATG AGGCAGCTGC TGTGCTATGT TCCATATGTG AGCGACCTCG GCTGGCCCAG 
CCTCCCAGCT TGGTGGTGGA TTCCCGAGAT GCTGGCATTT GCCTGCAACC CCTTCAGCAG 
GGGGATGCTT TGCTGGCCTC TGCCCAGAGT CAAGTCTGGT ACTGTATTCA CTGTACCTTC 
TGCAACTCGA GCCCTGGCTG GGTGTGTGTT ATGTGCAACC GGACTAGTAG CCCCATTCCA 
GCACAACATG CCCCCCGGCC CTATGCCAGC TCTTTGGAAA AGGGACCCCC CAAGCCTGGG 
CCCCCACGAC GCCTTAGTGC CCCCCTGCCC AGTTCCTGTG GAGATCCTGA GAAGCAGCGC 
CAAGACAAGA TGCGGGAAGA AGGCCTCCAG CTAGTGAGCA TGATCCGGGA AGGGGAAGCC 
GCAGGTGCCT GTCCAGAGGA GATCTTCTCG GCTCTGCAGT ACTCGGGCAC TGAGGTGCCT 
CTGCAGTGGT TGCGCTCAGA ACTGCCCTAC GTCCTGGAGA TGGTGGCTGA GCTGGCTGGA 
CAGCAGGACC CTGGGCTGGG TGCCTTTTCC TGTCAGGAGG CCCGGAGAGC CTGGCTGGAT 
CGTCATGGCA ACCTTGATGA AGCTGTGGAG GAGTGTGTGA GGACCAGGCG AAGGAAGGTG 
CAGGAGCTCC AGTCTCTAGG CTTTGGGCCT GAGGAGGGGT CTCTCCAGGC ATTGTTCCAG 
CACGGAGGTG ATGTGTCACG GGCCCTGACT GAGCTACAGC GCCAACGCCT AGAGCCCTTC 
CGCCAGCGCC TCTGGGACAG TGGCCCTGAG CCCACCCCTT CCTGGGATGG GCCAGACAAG 
CAGAGCCTGG TCAGGCGGCT TTTGGCAGTC TACGCACTCC CCAGCTGGGG CCGGGCAGAG 
CTGGCACTGT CACTGCTGCA GGAGACACCC AGGAACTATG AGTTGGGGGA TGTGGTAGAA 
GCTGTGAGGC ACAGCCAGGA CCGGGCCTTC CTGCGCCGCT TGCTTGCCCA GGAGTGTGCC 
GTGTGTGGCT GGGCCCTGCC CCACAACCGG ATGCAGGCCC TGACTTCCTG TGAGTGCACC 
ATCTGTCCTG ACTGCTTCCG CCAGCACTTC ACCATCGCCT TGAAGGAGAA GCACATCACA 
GACATGGTGT GCCCTGCCTG TGGCCGCCCC GACCTCACCG ATGACACACA GTTGCTCAGC 
TACTTCTCTA CCCTTGACAT CCAGCTTCGC GAGAGCCTAG AGCCAGATGC CTATGCGTTG 
TTCCATAAGA AGCTGACCGA GGGTGTGCTG ATGCGGGACC CCAAGTTCTT GTGGTGTGCC 
CAGTGCTCCT TTGGCTTCAT ATATGAGCGT GAGCAGCTGG AGGCAACTTG TCCCCAGTGT 
CACCAGACCT TCTGTGTGCG CTGCAAGCGC CAGTGGGAGG AGCAGCACCG AGGTCGGAGC 
TGTGAGGACT TCCAGAACTG GAAACGCATG AACGACCCAG AATACCAGGC CCAGGGCCTA 
GCAATGTATC TTCAGGAAAA CGGCATTGAC TGCCCCAAAT GCAAGTTCTC GTACGCCCTG 
GCCCGAGGAG GCTGCATGCA CTTTCACTGT ACCCAGTGCC GCCACCAGTT CTGCAGCGGC 
TGCTACAATG CCTTTTACGC CAAGAATAAA TGTCCAGAGC CTAACTGCAG GGTGAAAAAG 
TCCCTGCACG GCCACCACCC TCGAGACTGC CTCTTCTACC TGCGGGACTG GACTGCTCTC 
CGGCTTCAGA AGCTGCTACA GGACAATAAC GTCATGTTTA ATACAGAGCC TCCAGCTGGG 
GCCCGGGCAG TCCCTGGAGG CGGCTGCCGA GTGATAGAGC AGAAGGAGGT TCCCAATGGG 
CTCAGGGACG AAGCTTGTGG CAAGGAAACT CCAGCTGGCT ATGCCGGCCT GTGCCAGGCA 
CACTACAAAG AGTATCTTGT GAGCCTCATC AATGCCCACT CGCTGGACCC AGCCACCTTG 
TATGAGGTGG AAGAGCTGGA GACGGCCACT GAGCGCTACC TGCACGTACG CCCCCAGCCT 
TTGGCTGGAG AGGATCCCCC TGCTTACCAG GCCCGCTTGT TACAGAAGCT GACAGAAGAG 
GTACCCTTGG GACAGAGTAT CCCCCGCAGG CGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060469.4
CDS	202..3420
Translation	MPGEEEERAFLVAREELASALRRDSGQAFSLEQLRPLLASSLPLAARYLQLDAARLVRCNAHGEPRNYLNTLSTALNILEKYGRNLLSPQRPRYWRGVKFNNPVFRSTVDAVQGGRDVLRLYGYTEEQPDGLSFPEGQEEPDEHQVATVTLEVLLLRTELSLLLQNTHPRQQALEQLLEDKVEDDMLQLSEFDPLLREIAPGPLTTPSVPGSTPGPCFLCGSAPGTLHCPSCKQALCPACDHLFHGHPSRAHHLRQTLPGVLQGTHLSPSLPASAQPRPQSTSLLALGDSSLSSPNPASAHLPWHCAACAMLNEPWAVLCVACDRPRGCKGLGLGTEGPQGTGGLEPDLARGRWACQSCTFENEAAAVLCSICERPRLAQPPSLVVDSRDAGICLQPLQQGDALLASAQSQVWYCIHCTFCNSSPGWVCVMCNRTSSPIPAQHAPRPYASSLEKGPPKPGPPRRLSAPLPSSCGDPEKQRQDKMREEGLQLVSMIREGEAAGACPEEIFSALQYSGTEVPLQWLRSELPYVLEMVAELAGQQDPGLGAFSCQEARRAWLDRHGNLDEAVEECVRTRRRKVQELQSLGFGPEEGSLQALFQHGGDVSRALTELQRQRLEPFRQRLWDSGPEPTPSWDGPDKQSLVRRLLAVYALPSWGRAELALSLLQETPRNYELGDVVEAVRHSQDRAFLRRLLAQECAVCGWALPHNRMQALTSCECTICPDCFRQHFTIALKEKHITDMVCPACGRPDLTDDTQLLSYFSTLDIQLRESLEPDAYALFHKKLTEGVLMRDPKFLWCAQCSFGFIYEREQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRMNDPEYQAQGLAMYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPEPNCRVKKSLHGHHPRDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPGGGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVSLINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKLTEEVPLGQSIPRRRK

Target ORF information:

RefSeq Version	NM_017999.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 31 (RNF31), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017999.5

ORF Insert Sequence:

ATGCCGGGGG AGGAAGAGGA GCGGGCCTTC CTGGTGGCCC GCGAGGAGCT GGCGAGCGCC 
CTGAGGAGGG ATTCCGGGCA GGCGTTTTCC CTGGAGCAGC TCCGGCCGCT ACTAGCCAGC 
TCTCTGCCGC TAGCCGCCCG CTACCTGCAG CTGGACGCCG CACGCCTTGT CCGCTGCAAC 
GCTCATGGGG AGCCCCGAAA CTACCTCAAC ACCCTGTCCA CGGCTCTGAA CATCCTGGAG 
AAATACGGCC GCAACCTTCT CAGCCCTCAG CGGCCTCGGT ACTGGCGTGG TGTCAAGTTT 
AATAACCCTG TCTTTCGCAG CACGGTGGAT GCTGTGCAGG GGGGCCGAGA TGTGCTGCGA 
TTATATGGCT ACACAGAGGA GCAACCAGAT GGGTTGAGCT TCCCCGAAGG GCAGGAGGAG 
CCAGATGAGC ACCAGGTTGC TACAGTCACA CTGGAAGTAC TGCTGCTTCG GACAGAGCTC 
AGCCTGCTAT TGCAGAATAC TCATCCAAGA CAGCAGGCAC TGGAGCAGCT GTTGGAAGAC 
AAGGTTGAAG ATGATATGCT GCAGCTTTCA GAATTTGACC CCCTATTGAG AGAGATTGCT 
CCTGGCCCCC TCACCACACC CTCTGTCCCA GGCTCCACTC CTGGTCCCTG CTTCCTCTGT 
GGTTCTGCCC CAGGCACACT GCACTGCCCA TCCTGTAAAC AGGCCCTGTG TCCAGCCTGT 
GACCACCTGT TCCATGGACA CCCATCCCGT GCTCATCACC TCCGCCAGAC CCTGCCTGGG 
GTCCTGCAGG GTACCCACCT GAGCCCCAGT TTACCTGCCT CAGCCCAACC ACGGCCCCAG 
TCGACCTCCC TGCTGGCCCT GGGAGACAGC TCTCTTTCTT CCCCTAATCC TGCAAGTGCT 
CATTTGCCCT GGCACTGTGC TGCCTGTGCC ATGCTAAATG AGCCTTGGGC AGTGCTCTGT 
GTGGCCTGTG ATCGGCCCCG AGGCTGTAAG GGGTTGGGGT TGGGAACTGA GGGTCCCCAA 
GGAACTGGAG GCCTAGAACC TGATCTTGCA CGGGGTCGGT GGGCCTGCCA GAGCTGTACC 
TTTGAGAATG AGGCAGCTGC TGTGCTATGT TCCATATGTG AGCGACCTCG GCTGGCCCAG 
CCTCCCAGCT TGGTGGTGGA TTCCCGAGAT GCTGGCATTT GCCTGCAACC CCTTCAGCAG 
GGGGATGCTT TGCTGGCCTC TGCCCAGAGT CAAGTCTGGT ACTGTATTCA CTGTACCTTC 
TGCAACTCGA GCCCTGGCTG GGTGTGTGTT ATGTGCAACC GGACTAGTAG CCCCATTCCA 
GCACAACATG CCCCCCGGCC CTATGCCAGC TCTTTGGAAA AGGGACCCCC CAAGCCTGGG 
CCCCCACGAC GCCTTAGTGC CCCCCTGCCC AGTTCCTGTG GAGATCCTGA GAAGCAGCGC 
CAAGACAAGA TGCGGGAAGA AGGCCTCCAG CTAGTGAGCA TGATCCGGGA AGGGGAAGCC 
GCAGGTGCCT GTCCAGAGGA GATCTTCTCG GCTCTGCAGT ACTCGGGCAC TGAGGTGCCT 
CTGCAGTGGT TGCGCTCAGA ACTGCCCTAC GTCCTGGAGA TGGTGGCTGA GCTGGCTGGA 
CAGCAGGACC CTGGGCTGGG TGCCTTTTCC TGTCAGGAGG CCCGGAGAGC CTGGCTGGAT 
CGTCATGGCA ACCTTGATGA AGCTGTGGAG GAGTGTGTGA GGACCAGGCG AAGGAAGGTG 
CAGGAGCTCC AGTCTCTAGG CTTTGGGCCT GAGGAGGGGT CTCTCCAGGC ATTGTTCCAG 
CACGGAGGTG ATGTGTCACG GGCCCTGACT GAGCTACAGC GCCAACGCCT AGAGCCCTTC 
CGCCAGCGCC TCTGGGACAG TGGCCCTGAG CCCACCCCTT CCTGGGATGG GCCAGACAAG 
CAGAGCCTGG TCAGGCGGCT TTTGGCAGTC TACGCACTCC CCAGCTGGGG CCGGGCAGAG 
CTGGCACTGT CACTGCTGCA GGAGACACCC AGGAACTATG AGTTGGGGGA TGTGGTAGAA 
GCTGTGAGGC ACAGCCAGGA CCGGGCCTTC CTGCGCCGCT TGCTTGCCCA GGAGTGTGCC 
GTGTGTGGCT GGGCCCTGCC CCACAACCGG ATGCAGGCCC TGACTTCCTG TGAGTGCACC 
ATCTGTCCTG ACTGCTTCCG CCAGCACTTC ACCATCGCCT TGAAGGAGAA GCACATCACA 
GACATGGTGT GCCCTGCCTG TGGCCGCCCC GACCTCACCG ATGACACACA GTTGCTCAGC 
TACTTCTCTA CCCTTGACAT CCAGCTTCGC GAGAGCCTAG AGCCAGATGC CTATGCGTTG 
TTCCATAAGA AGCTGACCGA GGGTGTGCTG ATGCGGGACC CCAAGTTCTT GTGGTGTGCC 
CAGTGCTCCT TTGGCTTCAT ATATGAGCGT GAGCAGCTGG AGGCAACTTG TCCCCAGTGT 
CACCAGACCT TCTGTGTGCG CTGCAAGCGC CAGTGGGAGG AGCAGCACCG AGGTCGGAGC 
TGTGAGGACT TCCAGAACTG GAAACGCATG AACGACCCAG AATACCAGGC CCAGGGCCTA 
GCAATGTATC TTCAGGAAAA CGGCATTGAC TGCCCCAAAT GCAAGTTCTC GTACGCCCTG 
GCCCGAGGAG GCTGCATGCA CTTTCACTGT ACCCAGTGCC GCCACCAGTT CTGCAGCGGC 
TGCTACAATG CCTTTTACGC CAAGAATAAA TGTCCAGAGC CTAACTGCAG GGTGAAAAAG 
TCCCTGCACG GCCACCACCC TCGAGACTGC CTCTTCTACC TGCGGGACTG GACTGCTCTC 
CGGCTTCAGA AGCTGCTACA GGACAATAAC GTCATGTTTA ATACAGAGCC TCCAGCTGGG 
GCCCGGGCAG TCCCTGGAGG CGGCTGCCGA GTGATAGAGC AGAAGGAGGT TCCCAATGGG 
CTCAGGGACG AAGCTTGTGG CAAGGAAACT CCAGCTGGCT ATGCCGGCCT GTGCCAGGCA 
CACTACAAAG AGTATCTTGT GAGCCTCATC AATGCCCACT CGCTGGACCC AGCCACCTTG 
TATGAGGTGG AAGAGCTGGA GACGGCCACT GAGCGCTACC TGCACGTACG CCCCCAGCCT 
TTGGCTGGAG AGGATCCCCC TGCTTACCAG GCCCGCTTGT TACAGAAGCT GACAGAAGAG 
GTACCCTTGG GACAGAGTAT CCCCCGCAGG CGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF31 ( NM_017999.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060469.4 Homo sapiens ring finger protein 31 (RNF31), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25779
Clone ID Related Accession (Same CDS sequence)	NM_017999.5 , NM_017999.4
Accession Version	NM_017999.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-10
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF31 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN287790.1, BC012077.1 and CD742923.1. This sequence is a reference standard in the RefSeqGene project. On Jun 16, 2006 this sequence version replaced NM_017999.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225832.1, AK291247.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGGGG AGGAAGAGGA GCGGGCCTTC CTGGTGGCCC GCGAGGAGCT GGCGAGCGCC 
CTGAGGAGGG ATTCCGGGCA GGCGTTTTCC CTGGAGCAGC TCCGGCCGCT ACTAGCCAGC 
TCTCTGCCGC TAGCCGCCCG CTACCTGCAG CTGGACGCCG CACGCCTTGT CCGCTGCAAC 
GCTCATGGGG AGCCCCGAAA CTACCTCAAC ACCCTGTCCA CGGCTCTGAA CATCCTGGAG 
AAATACGGCC GCAACCTTCT CAGCCCTCAG CGGCCTCGGT ACTGGCGTGG TGTCAAGTTT 
AATAACCCTG TCTTTCGCAG CACGGTGGAT GCTGTGCAGG GGGGCCGAGA TGTGCTGCGA 
TTATATGGCT ACACAGAGGA GCAACCAGAT GGGTTGAGCT TCCCCGAAGG GCAGGAGGAG 
CCAGATGAGC ACCAGGTTGC TACAGTCACA CTGGAAGTAC TGCTGCTTCG GACAGAGCTC 
AGCCTGCTAT TGCAGAATAC TCATCCAAGA CAGCAGGCAC TGGAGCAGCT GTTGGAAGAC 
AAGGTTGAAG ATGATATGCT GCAGCTTTCA GAATTTGACC CCCTATTGAG AGAGATTGCT 
CCTGGCCCCC TCACCACACC CTCTGTCCCA GGCTCCACTC CTGGTCCCTG CTTCCTCTGT 
GGTTCTGCCC CAGGCACACT GCACTGCCCA TCCTGTAAAC AGGCCCTGTG TCCAGCCTGT 
GACCACCTGT TCCATGGACA CCCATCCCGT GCTCATCACC TCCGCCAGAC CCTGCCTGGG 
GTCCTGCAGG GTACCCACCT GAGCCCCAGT TTACCTGCCT CAGCCCAACC ACGGCCCCAG 
TCGACCTCCC TGCTGGCCCT GGGAGACAGC TCTCTTTCTT CCCCTAATCC TGCAAGTGCT 
CATTTGCCCT GGCACTGTGC TGCCTGTGCC ATGCTAAATG AGCCTTGGGC AGTGCTCTGT 
GTGGCCTGTG ATCGGCCCCG AGGCTGTAAG GGGTTGGGGT TGGGAACTGA GGGTCCCCAA 
GGAACTGGAG GCCTAGAACC TGATCTTGCA CGGGGTCGGT GGGCCTGCCA GAGCTGTACC 
TTTGAGAATG AGGCAGCTGC TGTGCTATGT TCCATATGTG AGCGACCTCG GCTGGCCCAG 
CCTCCCAGCT TGGTGGTGGA TTCCCGAGAT GCTGGCATTT GCCTGCAACC CCTTCAGCAG 
GGGGATGCTT TGCTGGCCTC TGCCCAGAGT CAAGTCTGGT ACTGTATTCA CTGTACCTTC 
TGCAACTCGA GCCCTGGCTG GGTGTGTGTT ATGTGCAACC GGACTAGTAG CCCCATTCCA 
GCACAACATG CCCCCCGGCC CTATGCCAGC TCTTTGGAAA AGGGACCCCC CAAGCCTGGG 
CCCCCACGAC GCCTTAGTGC CCCCCTGCCC AGTTCCTGTG GAGATCCTGA GAAGCAGCGC 
CAAGACAAGA TGCGGGAAGA AGGCCTCCAG CTAGTGAGCA TGATCCGGGA AGGGGAAGCC 
GCAGGTGCCT GTCCAGAGGA GATCTTCTCG GCTCTGCAGT ACTCGGGCAC TGAGGTGCCT 
CTGCAGTGGT TGCGCTCAGA ACTGCCCTAC GTCCTGGAGA TGGTGGCTGA GCTGGCTGGA 
CAGCAGGACC CTGGGCTGGG TGCCTTTTCC TGTCAGGAGG CCCGGAGAGC CTGGCTGGAT 
CGTCATGGCA ACCTTGATGA AGCTGTGGAG GAGTGTGTGA GGACCAGGCG AAGGAAGGTG 
CAGGAGCTCC AGTCTCTAGG CTTTGGGCCT GAGGAGGGGT CTCTCCAGGC ATTGTTCCAG 
CACGGAGGTG ATGTGTCACG GGCCCTGACT GAGCTACAGC GCCAACGCCT AGAGCCCTTC 
CGCCAGCGCC TCTGGGACAG TGGCCCTGAG CCCACCCCTT CCTGGGATGG GCCAGACAAG 
CAGAGCCTGG TCAGGCGGCT TTTGGCAGTC TACGCACTCC CCAGCTGGGG CCGGGCAGAG 
CTGGCACTGT CACTGCTGCA GGAGACACCC AGGAACTATG AGTTGGGGGA TGTGGTAGAA 
GCTGTGAGGC ACAGCCAGGA CCGGGCCTTC CTGCGCCGCT TGCTTGCCCA GGAGTGTGCC 
GTGTGTGGCT GGGCCCTGCC CCACAACCGG ATGCAGGCCC TGACTTCCTG TGAGTGCACC 
ATCTGTCCTG ACTGCTTCCG CCAGCACTTC ACCATCGCCT TGAAGGAGAA GCACATCACA 
GACATGGTGT GCCCTGCCTG TGGCCGCCCC GACCTCACCG ATGACACACA GTTGCTCAGC 
TACTTCTCTA CCCTTGACAT CCAGCTTCGC GAGAGCCTAG AGCCAGATGC CTATGCGTTG 
TTCCATAAGA AGCTGACCGA GGGTGTGCTG ATGCGGGACC CCAAGTTCTT GTGGTGTGCC 
CAGTGCTCCT TTGGCTTCAT ATATGAGCGT GAGCAGCTGG AGGCAACTTG TCCCCAGTGT 
CACCAGACCT TCTGTGTGCG CTGCAAGCGC CAGTGGGAGG AGCAGCACCG AGGTCGGAGC 
TGTGAGGACT TCCAGAACTG GAAACGCATG AACGACCCAG AATACCAGGC CCAGGGCCTA 
GCAATGTATC TTCAGGAAAA CGGCATTGAC TGCCCCAAAT GCAAGTTCTC GTACGCCCTG 
GCCCGAGGAG GCTGCATGCA CTTTCACTGT ACCCAGTGCC GCCACCAGTT CTGCAGCGGC 
TGCTACAATG CCTTTTACGC CAAGAATAAA TGTCCAGAGC CTAACTGCAG GGTGAAAAAG 
TCCCTGCACG GCCACCACCC TCGAGACTGC CTCTTCTACC TGCGGGACTG GACTGCTCTC 
CGGCTTCAGA AGCTGCTACA GGACAATAAC GTCATGTTTA ATACAGAGCC TCCAGCTGGG 
GCCCGGGCAG TCCCTGGAGG CGGCTGCCGA GTGATAGAGC AGAAGGAGGT TCCCAATGGG 
CTCAGGGACG AAGCTTGTGG CAAGGAAACT CCAGCTGGCT ATGCCGGCCT GTGCCAGGCA 
CACTACAAAG AGTATCTTGT GAGCCTCATC AATGCCCACT CGCTGGACCC AGCCACCTTG 
TATGAGGTGG AAGAGCTGGA GACGGCCACT GAGCGCTACC TGCACGTACG CCCCCAGCCT 
TTGGCTGGAG AGGATCCCCC TGCTTACCAG GCCCGCTTGT TACAGAAGCT GACAGAAGAG 
GTACCCTTGG GACAGAGTAT CCCCCGCAGG CGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060469.4
CDS	250..3468
Translation	MPGEEEERAFLVAREELASALRRDSGQAFSLEQLRPLLASSLPLAARYLQLDAARLVRCNAHGEPRNYLNTLSTALNILEKYGRNLLSPQRPRYWRGVKFNNPVFRSTVDAVQGGRDVLRLYGYTEEQPDGLSFPEGQEEPDEHQVATVTLEVLLLRTELSLLLQNTHPRQQALEQLLEDKVEDDMLQLSEFDPLLREIAPGPLTTPSVPGSTPGPCFLCGSAPGTLHCPSCKQALCPACDHLFHGHPSRAHHLRQTLPGVLQGTHLSPSLPASAQPRPQSTSLLALGDSSLSSPNPASAHLPWHCAACAMLNEPWAVLCVACDRPRGCKGLGLGTEGPQGTGGLEPDLARGRWACQSCTFENEAAAVLCSICERPRLAQPPSLVVDSRDAGICLQPLQQGDALLASAQSQVWYCIHCTFCNSSPGWVCVMCNRTSSPIPAQHAPRPYASSLEKGPPKPGPPRRLSAPLPSSCGDPEKQRQDKMREEGLQLVSMIREGEAAGACPEEIFSALQYSGTEVPLQWLRSELPYVLEMVAELAGQQDPGLGAFSCQEARRAWLDRHGNLDEAVEECVRTRRRKVQELQSLGFGPEEGSLQALFQHGGDVSRALTELQRQRLEPFRQRLWDSGPEPTPSWDGPDKQSLVRRLLAVYALPSWGRAELALSLLQETPRNYELGDVVEAVRHSQDRAFLRRLLAQECAVCGWALPHNRMQALTSCECTICPDCFRQHFTIALKEKHITDMVCPACGRPDLTDDTQLLSYFSTLDIQLRESLEPDAYALFHKKLTEGVLMRDPKFLWCAQCSFGFIYEREQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRMNDPEYQAQGLAMYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPEPNCRVKKSLHGHHPRDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPGGGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVSLINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKLTEEVPLGQSIPRRRK

Target ORF information:

RefSeq Version	NM_017999.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 31 (RNF31), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017999.4

ORF Insert Sequence:

ATGCCGGGGG AGGAAGAGGA GCGGGCCTTC CTGGTGGCCC GCGAGGAGCT GGCGAGCGCC 
CTGAGGAGGG ATTCCGGGCA GGCGTTTTCC CTGGAGCAGC TCCGGCCGCT ACTAGCCAGC 
TCTCTGCCGC TAGCCGCCCG CTACCTGCAG CTGGACGCCG CACGCCTTGT CCGCTGCAAC 
GCTCATGGGG AGCCCCGAAA CTACCTCAAC ACCCTGTCCA CGGCTCTGAA CATCCTGGAG 
AAATACGGCC GCAACCTTCT CAGCCCTCAG CGGCCTCGGT ACTGGCGTGG TGTCAAGTTT 
AATAACCCTG TCTTTCGCAG CACGGTGGAT GCTGTGCAGG GGGGCCGAGA TGTGCTGCGA 
TTATATGGCT ACACAGAGGA GCAACCAGAT GGGTTGAGCT TCCCCGAAGG GCAGGAGGAG 
CCAGATGAGC ACCAGGTTGC TACAGTCACA CTGGAAGTAC TGCTGCTTCG GACAGAGCTC 
AGCCTGCTAT TGCAGAATAC TCATCCAAGA CAGCAGGCAC TGGAGCAGCT GTTGGAAGAC 
AAGGTTGAAG ATGATATGCT GCAGCTTTCA GAATTTGACC CCCTATTGAG AGAGATTGCT 
CCTGGCCCCC TCACCACACC CTCTGTCCCA GGCTCCACTC CTGGTCCCTG CTTCCTCTGT 
GGTTCTGCCC CAGGCACACT GCACTGCCCA TCCTGTAAAC AGGCCCTGTG TCCAGCCTGT 
GACCACCTGT TCCATGGACA CCCATCCCGT GCTCATCACC TCCGCCAGAC CCTGCCTGGG 
GTCCTGCAGG GTACCCACCT GAGCCCCAGT TTACCTGCCT CAGCCCAACC ACGGCCCCAG 
TCGACCTCCC TGCTGGCCCT GGGAGACAGC TCTCTTTCTT CCCCTAATCC TGCAAGTGCT 
CATTTGCCCT GGCACTGTGC TGCCTGTGCC ATGCTAAATG AGCCTTGGGC AGTGCTCTGT 
GTGGCCTGTG ATCGGCCCCG AGGCTGTAAG GGGTTGGGGT TGGGAACTGA GGGTCCCCAA 
GGAACTGGAG GCCTAGAACC TGATCTTGCA CGGGGTCGGT GGGCCTGCCA GAGCTGTACC 
TTTGAGAATG AGGCAGCTGC TGTGCTATGT TCCATATGTG AGCGACCTCG GCTGGCCCAG 
CCTCCCAGCT TGGTGGTGGA TTCCCGAGAT GCTGGCATTT GCCTGCAACC CCTTCAGCAG 
GGGGATGCTT TGCTGGCCTC TGCCCAGAGT CAAGTCTGGT ACTGTATTCA CTGTACCTTC 
TGCAACTCGA GCCCTGGCTG GGTGTGTGTT ATGTGCAACC GGACTAGTAG CCCCATTCCA 
GCACAACATG CCCCCCGGCC CTATGCCAGC TCTTTGGAAA AGGGACCCCC CAAGCCTGGG 
CCCCCACGAC GCCTTAGTGC CCCCCTGCCC AGTTCCTGTG GAGATCCTGA GAAGCAGCGC 
CAAGACAAGA TGCGGGAAGA AGGCCTCCAG CTAGTGAGCA TGATCCGGGA AGGGGAAGCC 
GCAGGTGCCT GTCCAGAGGA GATCTTCTCG GCTCTGCAGT ACTCGGGCAC TGAGGTGCCT 
CTGCAGTGGT TGCGCTCAGA ACTGCCCTAC GTCCTGGAGA TGGTGGCTGA GCTGGCTGGA 
CAGCAGGACC CTGGGCTGGG TGCCTTTTCC TGTCAGGAGG CCCGGAGAGC CTGGCTGGAT 
CGTCATGGCA ACCTTGATGA AGCTGTGGAG GAGTGTGTGA GGACCAGGCG AAGGAAGGTG 
CAGGAGCTCC AGTCTCTAGG CTTTGGGCCT GAGGAGGGGT CTCTCCAGGC ATTGTTCCAG 
CACGGAGGTG ATGTGTCACG GGCCCTGACT GAGCTACAGC GCCAACGCCT AGAGCCCTTC 
CGCCAGCGCC TCTGGGACAG TGGCCCTGAG CCCACCCCTT CCTGGGATGG GCCAGACAAG 
CAGAGCCTGG TCAGGCGGCT TTTGGCAGTC TACGCACTCC CCAGCTGGGG CCGGGCAGAG 
CTGGCACTGT CACTGCTGCA GGAGACACCC AGGAACTATG AGTTGGGGGA TGTGGTAGAA 
GCTGTGAGGC ACAGCCAGGA CCGGGCCTTC CTGCGCCGCT TGCTTGCCCA GGAGTGTGCC 
GTGTGTGGCT GGGCCCTGCC CCACAACCGG ATGCAGGCCC TGACTTCCTG TGAGTGCACC 
ATCTGTCCTG ACTGCTTCCG CCAGCACTTC ACCATCGCCT TGAAGGAGAA GCACATCACA 
GACATGGTGT GCCCTGCCTG TGGCCGCCCC GACCTCACCG ATGACACACA GTTGCTCAGC 
TACTTCTCTA CCCTTGACAT CCAGCTTCGC GAGAGCCTAG AGCCAGATGC CTATGCGTTG 
TTCCATAAGA AGCTGACCGA GGGTGTGCTG ATGCGGGACC CCAAGTTCTT GTGGTGTGCC 
CAGTGCTCCT TTGGCTTCAT ATATGAGCGT GAGCAGCTGG AGGCAACTTG TCCCCAGTGT 
CACCAGACCT TCTGTGTGCG CTGCAAGCGC CAGTGGGAGG AGCAGCACCG AGGTCGGAGC 
TGTGAGGACT TCCAGAACTG GAAACGCATG AACGACCCAG AATACCAGGC CCAGGGCCTA 
GCAATGTATC TTCAGGAAAA CGGCATTGAC TGCCCCAAAT GCAAGTTCTC GTACGCCCTG 
GCCCGAGGAG GCTGCATGCA CTTTCACTGT ACCCAGTGCC GCCACCAGTT CTGCAGCGGC 
TGCTACAATG CCTTTTACGC CAAGAATAAA TGTCCAGAGC CTAACTGCAG GGTGAAAAAG 
TCCCTGCACG GCCACCACCC TCGAGACTGC CTCTTCTACC TGCGGGACTG GACTGCTCTC 
CGGCTTCAGA AGCTGCTACA GGACAATAAC GTCATGTTTA ATACAGAGCC TCCAGCTGGG 
GCCCGGGCAG TCCCTGGAGG CGGCTGCCGA GTGATAGAGC AGAAGGAGGT TCCCAATGGG 
CTCAGGGACG AAGCTTGTGG CAAGGAAACT CCAGCTGGCT ATGCCGGCCT GTGCCAGGCA 
CACTACAAAG AGTATCTTGT GAGCCTCATC AATGCCCACT CGCTGGACCC AGCCACCTTG 
TATGAGGTGG AAGAGCTGGA GACGGCCACT GAGCGCTACC TGCACGTACG CCCCCAGCCT 
TTGGCTGGAG AGGATCCCCC TGCTTACCAG GCCCGCTTGT TACAGAAGCT GACAGAAGAG 
GTACCCTTGG GACAGAGTAT CCCCCGCAGG CGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF31 ( NM_001310332.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001297261.1 Homo sapiens ring finger protein 31 (RNF31), transcript variant 2, mRNA.

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CloneID	OHu68946
Clone ID Related Accession (Same CDS sequence)	NM_001310332.1
Accession Version	NM_001310332.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF31 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA462121.1, AY256461.1, AL136295.3 and CD742923.1. Transcript Variant: This variant (2) uses an alternate first exon and an alternate splice site in a 5' exon compared to variant 1, resulting in the use of a downstream translation initiation site. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY256461.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144120, SAMEA2153733 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTTAT GCACCAGGGC TGGGGAGCCC AGCCTAACTC AAAATACTCA TCCAAGACAG 
CAGGCACTGG AGCAGCTGTT GGAAGACAAG GTTGAAGATG ATATGCTGCA GCTTTCAGAA 
TTTGACCCCC TATTGAGAGA GATTGCTCCT GGCCCCCTCA CCACACCCTC TGTCCCAGGC 
TCCACTCCTG GTCCCTGCTT CCTCTGTGGT TCTGCCCCAG GCACACTGCA CTGCCCATCC 
TGTAAACAGG CCCTGTGTCC AGCCTGTGAC CACCTGTTCC ATGGACACCC ATCCCGTGCT 
CATCACCTCC GCCAGACCCT GCCTGGGGTC CTGCAGGGTA CCCACCTGAG CCCCAGTTTA 
CCTGCCTCAG CCCAACCACG GCCCCAGTCG ACCTCCCTGC TGGCCCTGGG AGACAGCTCT 
CTTTCTTCCC CTAATCCTGC AAGTGCTCAT TTGCCCTGGC ACTGTGCTGC CTGTGCCATG 
CTAAATGAGC CTTGGGCAGT GCTCTGTGTG GCCTGTGATC GGCCCCGAGG CTGTAAGGGG 
TTGGGGTTGG GAACTGAGGG TCCCCAAGGA ACTGGAGGCC TAGAACCTGA TCTTGCACGG 
GGTCGGTGGG CCTGCCAGAG CTGTACCTTT GAGAATGAGG CAGCTGCTGT GCTATGTTCC 
ATATGTGAGC GACCTCGGCT GGCCCAGCCT CCCAGCTTGG TGGTGGATTC CCGAGATGCT 
GGCATTTGCC TGCAACCCCT TCAGCAGGGG GATGCTTTGC TGGCCTCTGC CCAGAGTCAA 
GTCTGGTACT GTATTCACTG TACCTTCTGC AACTCGAGCC CTGGCTGGGT GTGTGTTATG 
TGCAACCGGA CTAGTAGCCC CATTCCAGCA CAACATGCCC CCCGGCCCTA TGCCAGCTCT 
TTGGAAAAGG GACCCCCCAA GCCTGGGCCC CCACGACGCC TTAGTGCCCC CCTGCCCAGT 
TCCTGTGGAG ATCCTGAGAA GCAGCGCCAA GACAAGATGC GGGAAGAAGG CCTCCAGCTA 
GTGAGCATGA TCCGGGAAGG GGAAGCCGCA GGTGCCTGTC CAGAGGAGAT CTTCTCGGCT 
CTGCAGTACT CGGGCACTGA GGTGCCTCTG CAGTGGTTGC GCTCAGAACT GCCCTACGTC 
CTGGAGATGG TGGCTGAGCT GGCTGGACAG CAGGACCCTG GGCTGGGTGC CTTTTCCTGT 
CAGGAGGCCC GGAGAGCCTG GCTGGATCGT CATGGCAACC TTGATGAAGC TGTGGAGGAG 
TGTGTGAGGA CCAGGCGAAG GAAGGTGCAG GAGCTCCAGT CTCTAGGCTT TGGGCCTGAG 
GAGGGGTCTC TCCAGGCATT GTTCCAGCAC GGAGGTGATG TGTCACGGGC CCTGACTGAG 
CTACAGCGCC AACGCCTAGA GCCCTTCCGC CAGCGCCTCT GGGACAGTGG CCCTGAGCCC 
ACCCCTTCCT GGGATGGGCC AGACAAGCAG AGCCTGGTCA GGCGGCTTTT GGCAGTCTAC 
GCACTCCCCA GCTGGGGCCG GGCAGAGCTG GCACTGTCAC TGCTGCAGGA GACACCCAGG 
AACTATGAGT TGGGGGATGT GGTAGAAGCT GTGAGGCACA GCCAGGACCG GGCCTTCCTG 
CGCCGCTTGC TTGCCCAGGA GTGTGCCGTG TGTGGCTGGG CCCTGCCCCA CAACCGGATG 
CAGGCCCTGA CTTCCTGTGA GTGCACCATC TGTCCTGACT GCTTCCGCCA GCACTTCACC 
ATCGCCTTGA AGGAGAAGCA CATCACAGAC ATGGTGTGCC CTGCCTGTGG CCGCCCCGAC 
CTCACCGATG ACACACAGTT GCTCAGCTAC TTCTCTACCC TTGACATCCA GCTTCGCGAG 
AGCCTAGAGC CAGATGCCTA TGCGTTGTTC CATAAGAAGC TGACCGAGGG TGTGCTGATG 
CGGGACCCCA AGTTCTTGTG GTGTGCCCAG TGCTCCTTTG GCTTCATATA TGAGCGTGAG 
CAGCTGGAGG CAACTTGTCC CCAGTGTCAC CAGACCTTCT GTGTGCGCTG CAAGCGCCAG 
TGGGAGGAGC AGCACCGAGG TCGGAGCTGT GAGGACTTCC AGAACTGGAA ACGCATGAAC 
GACCCAGAAT ACCAGGCCCA GGGCCTAGCA ATGTATCTTC AGGAAAACGG CATTGACTGC 
CCCAAATGCA AGTTCTCGTA CGCCCTGGCC CGAGGAGGCT GCATGCACTT TCACTGTACC 
CAGTGCCGCC ACCAGTTCTG CAGCGGCTGC TACAATGCCT TTTACGCCAA GAATAAATGT 
CCAGAGCCTA ACTGCAGGGT GAAAAAGTCC CTGCACGGCC ACCACCCTCG AGACTGCCTC 
TTCTACCTGC GGGACTGGAC TGCTCTCCGG CTTCAGAAGC TGCTACAGGA CAATAACGTC 
ATGTTTAATA CAGAGCCTCC AGCTGGGGCC CGGGCAGTCC CTGGAGGCGG CTGCCGAGTG 
ATAGAGCAGA AGGAGGTTCC CAATGGGCTC AGGGACGAAG CTTGTGGCAA GGAAACTCCA 
GCTGGCTATG CCGGCCTGTG CCAGGCACAC TACAAAGAGT ATCTTGTGAG CCTCATCAAT 
GCCCACTCGC TGGACCCAGC CACCTTGTAT GAGGTGGAAG AGCTGGAGAC GGCCACTGAG 
CGCTACCTGC ACGTACGCCC CCAGCCTTTG GCTGGAGAGG ATCCCCCTGC TTACCAGGCC 
CGCTTGTTAC AGAAGCTGAC AGAAGAGGTA CCCTTGGGAC AGAGTATCCC CCGCAGGCGG 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001297261.1
CDS	359..3124
Translation	MDLCTRAGEPSLTQNTHPRQQALEQLLEDKVEDDMLQLSEFDPLLREIAPGPLTTPSVPGSTPGPCFLCGSAPGTLHCPSCKQALCPACDHLFHGHPSRAHHLRQTLPGVLQGTHLSPSLPASAQPRPQSTSLLALGDSSLSSPNPASAHLPWHCAACAMLNEPWAVLCVACDRPRGCKGLGLGTEGPQGTGGLEPDLARGRWACQSCTFENEAAAVLCSICERPRLAQPPSLVVDSRDAGICLQPLQQGDALLASAQSQVWYCIHCTFCNSSPGWVCVMCNRTSSPIPAQHAPRPYASSLEKGPPKPGPPRRLSAPLPSSCGDPEKQRQDKMREEGLQLVSMIREGEAAGACPEEIFSALQYSGTEVPLQWLRSELPYVLEMVAELAGQQDPGLGAFSCQEARRAWLDRHGNLDEAVEECVRTRRRKVQELQSLGFGPEEGSLQALFQHGGDVSRALTELQRQRLEPFRQRLWDSGPEPTPSWDGPDKQSLVRRLLAVYALPSWGRAELALSLLQETPRNYELGDVVEAVRHSQDRAFLRRLLAQECAVCGWALPHNRMQALTSCECTICPDCFRQHFTIALKEKHITDMVCPACGRPDLTDDTQLLSYFSTLDIQLRESLEPDAYALFHKKLTEGVLMRDPKFLWCAQCSFGFIYEREQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRMNDPEYQAQGLAMYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPEPNCRVKKSLHGHHPRDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPGGGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVSLINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKLTEEVPLGQSIPRRRK

Target ORF information:

RefSeq Version	NM_001310332.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 31 (RNF31), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310332.1

ORF Insert Sequence:

ATGGACTTAT GCACCAGGGC TGGGGAGCCC AGCCTAACTC AAAATACTCA TCCAAGACAG 
CAGGCACTGG AGCAGCTGTT GGAAGACAAG GTTGAAGATG ATATGCTGCA GCTTTCAGAA 
TTTGACCCCC TATTGAGAGA GATTGCTCCT GGCCCCCTCA CCACACCCTC TGTCCCAGGC 
TCCACTCCTG GTCCCTGCTT CCTCTGTGGT TCTGCCCCAG GCACACTGCA CTGCCCATCC 
TGTAAACAGG CCCTGTGTCC AGCCTGTGAC CACCTGTTCC ATGGACACCC ATCCCGTGCT 
CATCACCTCC GCCAGACCCT GCCTGGGGTC CTGCAGGGTA CCCACCTGAG CCCCAGTTTA 
CCTGCCTCAG CCCAACCACG GCCCCAGTCG ACCTCCCTGC TGGCCCTGGG AGACAGCTCT 
CTTTCTTCCC CTAATCCTGC AAGTGCTCAT TTGCCCTGGC ACTGTGCTGC CTGTGCCATG 
CTAAATGAGC CTTGGGCAGT GCTCTGTGTG GCCTGTGATC GGCCCCGAGG CTGTAAGGGG 
TTGGGGTTGG GAACTGAGGG TCCCCAAGGA ACTGGAGGCC TAGAACCTGA TCTTGCACGG 
GGTCGGTGGG CCTGCCAGAG CTGTACCTTT GAGAATGAGG CAGCTGCTGT GCTATGTTCC 
ATATGTGAGC GACCTCGGCT GGCCCAGCCT CCCAGCTTGG TGGTGGATTC CCGAGATGCT 
GGCATTTGCC TGCAACCCCT TCAGCAGGGG GATGCTTTGC TGGCCTCTGC CCAGAGTCAA 
GTCTGGTACT GTATTCACTG TACCTTCTGC AACTCGAGCC CTGGCTGGGT GTGTGTTATG 
TGCAACCGGA CTAGTAGCCC CATTCCAGCA CAACATGCCC CCCGGCCCTA TGCCAGCTCT 
TTGGAAAAGG GACCCCCCAA GCCTGGGCCC CCACGACGCC TTAGTGCCCC CCTGCCCAGT 
TCCTGTGGAG ATCCTGAGAA GCAGCGCCAA GACAAGATGC GGGAAGAAGG CCTCCAGCTA 
GTGAGCATGA TCCGGGAAGG GGAAGCCGCA GGTGCCTGTC CAGAGGAGAT CTTCTCGGCT 
CTGCAGTACT CGGGCACTGA GGTGCCTCTG CAGTGGTTGC GCTCAGAACT GCCCTACGTC 
CTGGAGATGG TGGCTGAGCT GGCTGGACAG CAGGACCCTG GGCTGGGTGC CTTTTCCTGT 
CAGGAGGCCC GGAGAGCCTG GCTGGATCGT CATGGCAACC TTGATGAAGC TGTGGAGGAG 
TGTGTGAGGA CCAGGCGAAG GAAGGTGCAG GAGCTCCAGT CTCTAGGCTT TGGGCCTGAG 
GAGGGGTCTC TCCAGGCATT GTTCCAGCAC GGAGGTGATG TGTCACGGGC CCTGACTGAG 
CTACAGCGCC AACGCCTAGA GCCCTTCCGC CAGCGCCTCT GGGACAGTGG CCCTGAGCCC 
ACCCCTTCCT GGGATGGGCC AGACAAGCAG AGCCTGGTCA GGCGGCTTTT GGCAGTCTAC 
GCACTCCCCA GCTGGGGCCG GGCAGAGCTG GCACTGTCAC TGCTGCAGGA GACACCCAGG 
AACTATGAGT TGGGGGATGT GGTAGAAGCT GTGAGGCACA GCCAGGACCG GGCCTTCCTG 
CGCCGCTTGC TTGCCCAGGA GTGTGCCGTG TGTGGCTGGG CCCTGCCCCA CAACCGGATG 
CAGGCCCTGA CTTCCTGTGA GTGCACCATC TGTCCTGACT GCTTCCGCCA GCACTTCACC 
ATCGCCTTGA AGGAGAAGCA CATCACAGAC ATGGTGTGCC CTGCCTGTGG CCGCCCCGAC 
CTCACCGATG ACACACAGTT GCTCAGCTAC TTCTCTACCC TTGACATCCA GCTTCGCGAG 
AGCCTAGAGC CAGATGCCTA TGCGTTGTTC CATAAGAAGC TGACCGAGGG TGTGCTGATG 
CGGGACCCCA AGTTCTTGTG GTGTGCCCAG TGCTCCTTTG GCTTCATATA TGAGCGTGAG 
CAGCTGGAGG CAACTTGTCC CCAGTGTCAC CAGACCTTCT GTGTGCGCTG CAAGCGCCAG 
TGGGAGGAGC AGCACCGAGG TCGGAGCTGT GAGGACTTCC AGAACTGGAA ACGCATGAAC 
GACCCAGAAT ACCAGGCCCA GGGCCTAGCA ATGTATCTTC AGGAAAACGG CATTGACTGC 
CCCAAATGCA AGTTCTCGTA CGCCCTGGCC CGAGGAGGCT GCATGCACTT TCACTGTACC 
CAGTGCCGCC ACCAGTTCTG CAGCGGCTGC TACAATGCCT TTTACGCCAA GAATAAATGT 
CCAGAGCCTA ACTGCAGGGT GAAAAAGTCC CTGCACGGCC ACCACCCTCG AGACTGCCTC 
TTCTACCTGC GGGACTGGAC TGCTCTCCGG CTTCAGAAGC TGCTACAGGA CAATAACGTC 
ATGTTTAATA CAGAGCCTCC AGCTGGGGCC CGGGCAGTCC CTGGAGGCGG CTGCCGAGTG 
ATAGAGCAGA AGGAGGTTCC CAATGGGCTC AGGGACGAAG CTTGTGGCAA GGAAACTCCA 
GCTGGCTATG CCGGCCTGTG CCAGGCACAC TACAAAGAGT ATCTTGTGAG CCTCATCAAT 
GCCCACTCGC TGGACCCAGC CACCTTGTAT GAGGTGGAAG AGCTGGAGAC GGCCACTGAG 
CGCTACCTGC ACGTACGCCC CCAGCCTTTG GCTGGAGAGG ATCCCCCTGC TTACCAGGCC 
CGCTTGTTAC AGAAGCTGAC AGAAGAGGTA CCCTTGGGAC AGAGTATCCC CCGCAGGCGG 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural Insights into SHARPIN-Mediated Activation of HOIP for the Linear Ubiquitin Chain Assembly.
Cell reports21(1)27-36(2017 Oct)
Liu J,Wang Y,Gong Y,Fu T,Hu S,Zhou Z,Pan L

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

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RNF31 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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