PPP1CB cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPP1CB
Entrez Gene ID	5500
Full Name	protein phosphatase 1 catalytic subunit beta
Synonyms	HEL-S-80p,NSLH2,PP-1B,PP1B,PP1beta,PPP1CD
General protein information	Preferred Names protein phosphatase 1 catalytic subunit beta Names serine/threonine-protein phosphatase PP1-beta catalytic subunit epididymis secretory sperm binding protein Li 80p protein phosphatase 1, catalytic subunit, beta isoform protein phosphatase 1, catalytic subunit, beta isozyme protein phosphatase 1, catalytic subunit, delta isoform protein phosphatase 1-beta protein phosphatase 1-delta
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p23.2
Summary	The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008].
Disorder MIM:	600590
Disorder Html:	Noonan syndrome-like disorder with loose anagen hair 2, 617506 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_206876.1	NP_996759.1	serine/threonine-protein phosphatase PP1-beta catalytic subunit isoform 1
NM_002709.2	NP_002700.1	serine/threonine-protein phosphatase PP1-beta catalytic subunit isoform 1
NM_002709.3	NP_002700.1	serine/threonine-protein phosphatase PP1-beta catalytic subunit isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PPP1CB	NP_996759.1
Pan troglodytes (chimpanzee)	PPP1CB	XP_001161723.1
	PPP1CB	XP_001101844.1
Rattus norvegicus (Norway rat)	Ppp1cb	NP_037197.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003114	XP_312797.5
Mus musculus (house mouse)	Ppp1cb	NP_766295.2
Xenopus tropicalis (tropical clawed frog)	ppp1cb	NP_001011467.1
Bos taurus (cattle)	PPP1CB	NP_001029825.1
Gallus gallus (chicken)	PPP1CB	NP_990453.1
Danio rerio (zebrafish)	ppp1cb	NP_001004527.2
Arabidopsis thaliana (thale cress)	TOPP7	NP_567375.1
Oryza sativa (rice)	Os06g0164100	NP_001056901.1
Canis lupus familiaris (dog)	PPP1CB	NP_001003034.1
Drosophila melanogaster (fruit fly)	flw	NP_001259407.1
Caenorhabditis elegans (roundworm)	gsp-1	NP_001256250.1
Arabidopsis thaliana (thale cress)	TOPP3	NP_176587.1

Gene Ontology
Bibliography

Related articles in PubMed

PAI1: a novel PP1-interacting protein that mediates human plasma's anti-apoptotic effect in endothelial cells.
Yao H, He G, Chen C, Yan S, Lu L, Song L, Vijayan KV, Li Q, Xiong L, Miao X, Deng X
Journal of cellular and molecular medicine21(9)2068-2076(2017 Sep)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Systematic Analysis of Human Protein Phosphatase Interactions and Dynamics.
Yadav L, Tamene F, G??s H, van Drogen A, Katainen R, Aebersold R, Gstaiger M, Varjosalo M
Cell systems4(4)430-444.e5(2017 Apr)

The recurrent PPP1CB mutation p.Pro49Arg in an additional Noonan-like syndrome individual: Broadening the clinical phenotype.
Bertola D, Yamamoto G, Buscarilli M, Jorge A, Passos-Bueno MR, Kim C
American journal of medical genetics. Part A173(3)824-828(2017 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This report gives further support that this novel RASopathy-PPP1CB-related Noonan syndrome with loose anagen hair-shares great similarity to Noonan syndrome-like disorder with loose anagen hair, and expands the phenotypic spectrum by adding the cranial vault abnormality.
Title: The recurrent PPP1CB mutation p.Pro49Arg in an additional Noonan-like syndrome individual: Broadening the clinical phenotype.

Our data suggest that our heterozygous de novo PPP1CB pathogenic variants are associated with syndromic intellectual disability.
Title: De novo missense variants in PPP1CB are associated with intellectual disability and congenital heart disease.

this study shows a pivotal role for PP1 in impeding IRF7-mediated IFN-alpha production in host immune responses
Title: Protein phosphatase 1 abrogates IRF7-mediated type I IFN response in antiviral immunity.

Missense mutations in PPP1CB are associated with a phenotype resembling NS-LAH. Based on this phenotype and PPP1CB's known role within the RAS/MAPK pathway, we think that this condition is a novel rasopathy, which may be provisionally termed "PPP1CB-related Noonan syndrome with loose anagen hair" (P-NS-LAH).
Title: A novel rasopathy caused by recurrent de novo missense mutations in PPP1CB closely resembles Noonan syndrome with loose anagen hair.

These results suggest a unique functional role for the PP1beta isoform in affecting cardiac contractile function
Title: Cardiac-specific deletion of protein phosphatase 1? promotes increased myofilament protein phosphorylation and contractile alterations.

The following PPP1CB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPP1CB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03032	NM_002709.3 Latest version!	Homo sapiens protein phosphatase 1 catalytic subunit beta (PPP1CB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu03032	NM_206876.1 Latest version!	Homo sapiens protein phosphatase 1 catalytic subunit beta (PPP1CB), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu03032	NM_002709.2	Homo sapiens protein phosphatase 1 catalytic subunit beta (PPP1CB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPP1CB ( NM_206876.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_996759.1 Homo sapiens protein phosphatase 1 catalytic subunit beta (PPP1CB), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu03032
Clone ID Related Accession (Same CDS sequence)	NM_206876.1 , NM_002709.2 , NM_002709.3
Accession Version	NM_206876.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase PP1-beta catalytic subunit isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI551898.1, CD656807.1, AF092905.1, CB111140.1, X80910.1, BQ933435.1, BM835970.1, CD722576.1 and BE047402.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Both variants encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.107990.1, SRR1803616.6694.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACG GGGAGCTGAA CGTGGACAGC CTCATCACCC GGCTGCTGGA GGTACGAGGA 
TGTCGTCCAG GAAAGATTGT GCAGATGACT GAAGCAGAAG TTCGAGGCTT ATGTATCAAG 
TCTCGGGAGA TCTTTCTCAG CCAGCCTATT CTTTTGGAAT TGGAAGCACC GCTGAAAATT 
TGTGGAGATA TTCATGGACA ATATACAGAT TTACTGAGAT TATTTGAATA TGGAGGTTTC 
CCACCAGAAG CCAACTATCT TTTCTTAGGA GATTATGTGG ACAGAGGAAA GCAGTCTTTG 
GAAACCATTT GTTTGCTATT GGCTTATAAA ATCAAATATC CAGAGAACTT CTTTCTCTTA 
AGAGGAAACC ATGAGTGTGC TAGCATCAAT CGCATTTATG GATTCTATGA TGAATGCAAA 
CGAAGATTTA ATATTAAATT GTGGAAGACC TTCACTGATT GTTTTAACTG TCTGCCTATA 
GCAGCCATTG TGGATGAGAA GATCTTCTGT TGTCATGGAG GATTGTCACC AGACCTGCAA 
TCTATGGAGC AGATTCGGAG AATTATGAGA CCTACTGATG TCCCTGATAC AGGTTTGCTC 
TGTGATTTGC TATGGTCTGA TCCAGATAAG GATGTGCAAG GCTGGGGAGA AAATGATCGT 
GGTGTTTCCT TTACTTTTGG AGCTGATGTA GTCAGTAAAT TTCTGAATCG TCATGATTTA 
GATTTGATTT GTCGAGCTCA TCAGGTGGTG GAAGATGGAT ATGAATTTTT TGCTAAACGA 
CAGTTGGTAA CCTTATTTTC AGCCCCAAAT TACTGTGGCG AGTTTGATAA TGCTGGTGGA 
ATGATGAGTG TGGATGAAAC TTTGATGTGT TCATTTCAGA TATTGAAACC ATCTGAAAAG 
AAAGCTAAAT ACCAGTATGG TGGACTGAAT TCTGGACGTC CTGTCACTCC ACCTCGAACA 
GCTAATCCGC CGAAGAAAAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996759.1
CDS	161..1144
Translation	MADGELNVDSLITRLLEVRGCRPGKIVQMTEAEVRGLCIKSREIFLSQPILLELEAPLKICGDIHGQYTDLLRLFEYGGFPPEANYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRFNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDTGLLCDLLWSDPDKDVQGWGENDRGVSFTFGADVVSKFLNRHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGGMMSVDETLMCSFQILKPSEKKAKYQYGGLNSGRPVTPPRTANPPKKR

Target ORF information:

RefSeq Version	NM_206876.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 1 catalytic subunit beta (PPP1CB), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206876.1

ORF Insert Sequence:

ATGGCGGACG GGGAGCTGAA CGTGGACAGC CTCATCACCC GGCTGCTGGA GGTACGAGGA 
TGTCGTCCAG GAAAGATTGT GCAGATGACT GAAGCAGAAG TTCGAGGCTT ATGTATCAAG 
TCTCGGGAGA TCTTTCTCAG CCAGCCTATT CTTTTGGAAT TGGAAGCACC GCTGAAAATT 
TGTGGAGATA TTCATGGACA ATATACAGAT TTACTGAGAT TATTTGAATA TGGAGGTTTC 
CCACCAGAAG CCAACTATCT TTTCTTAGGA GATTATGTGG ACAGAGGAAA GCAGTCTTTG 
GAAACCATTT GTTTGCTATT GGCTTATAAA ATCAAATATC CAGAGAACTT CTTTCTCTTA 
AGAGGAAACC ATGAGTGTGC TAGCATCAAT CGCATTTATG GATTCTATGA TGAATGCAAA 
CGAAGATTTA ATATTAAATT GTGGAAGACC TTCACTGATT GTTTTAACTG TCTGCCTATA 
GCAGCCATTG TGGATGAGAA GATCTTCTGT TGTCATGGAG GATTGTCACC AGACCTGCAA 
TCTATGGAGC AGATTCGGAG AATTATGAGA CCTACTGATG TCCCTGATAC AGGTTTGCTC 
TGTGATTTGC TATGGTCTGA TCCAGATAAG GATGTGCAAG GCTGGGGAGA AAATGATCGT 
GGTGTTTCCT TTACTTTTGG AGCTGATGTA GTCAGTAAAT TTCTGAATCG TCATGATTTA 
GATTTGATTT GTCGAGCTCA TCAGGTGGTG GAAGATGGAT ATGAATTTTT TGCTAAACGA 
CAGTTGGTAA CCTTATTTTC AGCCCCAAAT TACTGTGGCG AGTTTGATAA TGCTGGTGGA 
ATGATGAGTG TGGATGAAAC TTTGATGTGT TCATTTCAGA TATTGAAACC ATCTGAAAAG 
AAAGCTAAAT ACCAGTATGG TGGACTGAAT TCTGGACGTC CTGTCACTCC ACCTCGAACA 
GCTAATCCGC CGAAGAAAAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP1CB ( NM_002709.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_002700.1 Homo sapiens protein phosphatase 1 catalytic subunit beta (PPP1CB), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03032
Clone ID Related Accession (Same CDS sequence)	NM_206876.1 , NM_002709.2 , NM_002709.3
Accession Version	NM_002709.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase PP1-beta catalytic subunit isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI551898.1, BX477003.1, BC002697.2, X80910.1, BX647295.1, BM835970.1, CD722576.1 and BE047402.1. On Apr 6, 2004 this sequence version replaced NM_002709.1. Transcript Variant: This variant (1) represents the longest transcript. It encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.191333.1, SRR1660809.235384.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACG GGGAGCTGAA CGTGGACAGC CTCATCACCC GGCTGCTGGA GGTACGAGGA 
TGTCGTCCAG GAAAGATTGT GCAGATGACT GAAGCAGAAG TTCGAGGCTT ATGTATCAAG 
TCTCGGGAGA TCTTTCTCAG CCAGCCTATT CTTTTGGAAT TGGAAGCACC GCTGAAAATT 
TGTGGAGATA TTCATGGACA ATATACAGAT TTACTGAGAT TATTTGAATA TGGAGGTTTC 
CCACCAGAAG CCAACTATCT TTTCTTAGGA GATTATGTGG ACAGAGGAAA GCAGTCTTTG 
GAAACCATTT GTTTGCTATT GGCTTATAAA ATCAAATATC CAGAGAACTT CTTTCTCTTA 
AGAGGAAACC ATGAGTGTGC TAGCATCAAT CGCATTTATG GATTCTATGA TGAATGCAAA 
CGAAGATTTA ATATTAAATT GTGGAAGACC TTCACTGATT GTTTTAACTG TCTGCCTATA 
GCAGCCATTG TGGATGAGAA GATCTTCTGT TGTCATGGAG GATTGTCACC AGACCTGCAA 
TCTATGGAGC AGATTCGGAG AATTATGAGA CCTACTGATG TCCCTGATAC AGGTTTGCTC 
TGTGATTTGC TATGGTCTGA TCCAGATAAG GATGTGCAAG GCTGGGGAGA AAATGATCGT 
GGTGTTTCCT TTACTTTTGG AGCTGATGTA GTCAGTAAAT TTCTGAATCG TCATGATTTA 
GATTTGATTT GTCGAGCTCA TCAGGTGGTG GAAGATGGAT ATGAATTTTT TGCTAAACGA 
CAGTTGGTAA CCTTATTTTC AGCCCCAAAT TACTGTGGCG AGTTTGATAA TGCTGGTGGA 
ATGATGAGTG TGGATGAAAC TTTGATGTGT TCATTTCAGA TATTGAAACC ATCTGAAAAG 
AAAGCTAAAT ACCAGTATGG TGGACTGAAT TCTGGACGTC CTGTCACTCC ACCTCGAACA 
GCTAATCCGC CGAAGAAAAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002700.1
CDS	366..1349
Translation	MADGELNVDSLITRLLEVRGCRPGKIVQMTEAEVRGLCIKSREIFLSQPILLELEAPLKICGDIHGQYTDLLRLFEYGGFPPEANYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRFNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDTGLLCDLLWSDPDKDVQGWGENDRGVSFTFGADVVSKFLNRHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGGMMSVDETLMCSFQILKPSEKKAKYQYGGLNSGRPVTPPRTANPPKKR

Target ORF information:

RefSeq Version	NM_002709.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 1 catalytic subunit beta (PPP1CB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002709.2

ORF Insert Sequence:

ATGGCGGACG GGGAGCTGAA CGTGGACAGC CTCATCACCC GGCTGCTGGA GGTACGAGGA 
TGTCGTCCAG GAAAGATTGT GCAGATGACT GAAGCAGAAG TTCGAGGCTT ATGTATCAAG 
TCTCGGGAGA TCTTTCTCAG CCAGCCTATT CTTTTGGAAT TGGAAGCACC GCTGAAAATT 
TGTGGAGATA TTCATGGACA ATATACAGAT TTACTGAGAT TATTTGAATA TGGAGGTTTC 
CCACCAGAAG CCAACTATCT TTTCTTAGGA GATTATGTGG ACAGAGGAAA GCAGTCTTTG 
GAAACCATTT GTTTGCTATT GGCTTATAAA ATCAAATATC CAGAGAACTT CTTTCTCTTA 
AGAGGAAACC ATGAGTGTGC TAGCATCAAT CGCATTTATG GATTCTATGA TGAATGCAAA 
CGAAGATTTA ATATTAAATT GTGGAAGACC TTCACTGATT GTTTTAACTG TCTGCCTATA 
GCAGCCATTG TGGATGAGAA GATCTTCTGT TGTCATGGAG GATTGTCACC AGACCTGCAA 
TCTATGGAGC AGATTCGGAG AATTATGAGA CCTACTGATG TCCCTGATAC AGGTTTGCTC 
TGTGATTTGC TATGGTCTGA TCCAGATAAG GATGTGCAAG GCTGGGGAGA AAATGATCGT 
GGTGTTTCCT TTACTTTTGG AGCTGATGTA GTCAGTAAAT TTCTGAATCG TCATGATTTA 
GATTTGATTT GTCGAGCTCA TCAGGTGGTG GAAGATGGAT ATGAATTTTT TGCTAAACGA 
CAGTTGGTAA CCTTATTTTC AGCCCCAAAT TACTGTGGCG AGTTTGATAA TGCTGGTGGA 
ATGATGAGTG TGGATGAAAC TTTGATGTGT TCATTTCAGA TATTGAAACC ATCTGAAAAG 
AAAGCTAAAT ACCAGTATGG TGGACTGAAT TCTGGACGTC CTGTCACTCC ACCTCGAACA 
GCTAATCCGC CGAAGAAAAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP1CB ( NM_002709.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002700.1 Homo sapiens protein phosphatase 1 catalytic subunit beta (PPP1CB), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03032
Clone ID Related Accession (Same CDS sequence)	NM_206876.1 , NM_002709.2 , NM_002709.3
Accession Version	NM_002709.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase PP1-beta catalytic subunit isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX477003.1, BC002697.2, X80910.1, BX647295.1, BM835970.1, CD722576.1 and BE047402.1. On Nov 22, 2018 this sequence version replaced NM_002709.2. Transcript Variant: This variant (1) represents the longest transcript. It encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.191333.1, SRR1660809.235384.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000395366.3/ ENSP00000378769.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGACG GGGAGCTGAA CGTGGACAGC CTCATCACCC GGCTGCTGGA GGTACGAGGA 
TGTCGTCCAG GAAAGATTGT GCAGATGACT GAAGCAGAAG TTCGAGGCTT ATGTATCAAG 
TCTCGGGAGA TCTTTCTCAG CCAGCCTATT CTTTTGGAAT TGGAAGCACC GCTGAAAATT 
TGTGGAGATA TTCATGGACA ATATACAGAT TTACTGAGAT TATTTGAATA TGGAGGTTTC 
CCACCAGAAG CCAACTATCT TTTCTTAGGA GATTATGTGG ACAGAGGAAA GCAGTCTTTG 
GAAACCATTT GTTTGCTATT GGCTTATAAA ATCAAATATC CAGAGAACTT CTTTCTCTTA 
AGAGGAAACC ATGAGTGTGC TAGCATCAAT CGCATTTATG GATTCTATGA TGAATGCAAA 
CGAAGATTTA ATATTAAATT GTGGAAGACC TTCACTGATT GTTTTAACTG TCTGCCTATA 
GCAGCCATTG TGGATGAGAA GATCTTCTGT TGTCATGGAG GATTGTCACC AGACCTGCAA 
TCTATGGAGC AGATTCGGAG AATTATGAGA CCTACTGATG TCCCTGATAC AGGTTTGCTC 
TGTGATTTGC TATGGTCTGA TCCAGATAAG GATGTGCAAG GCTGGGGAGA AAATGATCGT 
GGTGTTTCCT TTACTTTTGG AGCTGATGTA GTCAGTAAAT TTCTGAATCG TCATGATTTA 
GATTTGATTT GTCGAGCTCA TCAGGTGGTG GAAGATGGAT ATGAATTTTT TGCTAAACGA 
CAGTTGGTAA CCTTATTTTC AGCCCCAAAT TACTGTGGCG AGTTTGATAA TGCTGGTGGA 
ATGATGAGTG TGGATGAAAC TTTGATGTGT TCATTTCAGA TATTGAAACC ATCTGAAAAG 
AAAGCTAAAT ACCAGTATGG TGGACTGAAT TCTGGACGTC CTGTCACTCC ACCTCGAACA 
GCTAATCCGC CGAAGAAAAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002700.1
CDS	296..1279
Translation	MADGELNVDSLITRLLEVRGCRPGKIVQMTEAEVRGLCIKSREIFLSQPILLELEAPLKICGDIHGQYTDLLRLFEYGGFPPEANYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRFNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDTGLLCDLLWSDPDKDVQGWGENDRGVSFTFGADVVSKFLNRHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGGMMSVDETLMCSFQILKPSEKKAKYQYGGLNSGRPVTPPRTANPPKKR

Target ORF information:

RefSeq Version	NM_002709.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 1 catalytic subunit beta (PPP1CB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002709.3

ORF Insert Sequence:

ATGGCGGACG GGGAGCTGAA CGTGGACAGC CTCATCACCC GGCTGCTGGA GGTACGAGGA 
TGTCGTCCAG GAAAGATTGT GCAGATGACT GAAGCAGAAG TTCGAGGCTT ATGTATCAAG 
TCTCGGGAGA TCTTTCTCAG CCAGCCTATT CTTTTGGAAT TGGAAGCACC GCTGAAAATT 
TGTGGAGATA TTCATGGACA ATATACAGAT TTACTGAGAT TATTTGAATA TGGAGGTTTC 
CCACCAGAAG CCAACTATCT TTTCTTAGGA GATTATGTGG ACAGAGGAAA GCAGTCTTTG 
GAAACCATTT GTTTGCTATT GGCTTATAAA ATCAAATATC CAGAGAACTT CTTTCTCTTA 
AGAGGAAACC ATGAGTGTGC TAGCATCAAT CGCATTTATG GATTCTATGA TGAATGCAAA 
CGAAGATTTA ATATTAAATT GTGGAAGACC TTCACTGATT GTTTTAACTG TCTGCCTATA 
GCAGCCATTG TGGATGAGAA GATCTTCTGT TGTCATGGAG GATTGTCACC AGACCTGCAA 
TCTATGGAGC AGATTCGGAG AATTATGAGA CCTACTGATG TCCCTGATAC AGGTTTGCTC 
TGTGATTTGC TATGGTCTGA TCCAGATAAG GATGTGCAAG GCTGGGGAGA AAATGATCGT 
GGTGTTTCCT TTACTTTTGG AGCTGATGTA GTCAGTAAAT TTCTGAATCG TCATGATTTA 
GATTTGATTT GTCGAGCTCA TCAGGTGGTG GAAGATGGAT ATGAATTTTT TGCTAAACGA 
CAGTTGGTAA CCTTATTTTC AGCCCCAAAT TACTGTGGCG AGTTTGATAA TGCTGGTGGA 
ATGATGAGTG TGGATGAAAC TTTGATGTGT TCATTTCAGA TATTGAAACC ATCTGAAAAG 
AAAGCTAAAT ACCAGTATGG TGGACTGAAT TCTGGACGTC CTGTCACTCC ACCTCGAACA 
GCTAATCCGC CGAAGAAAAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PAI1: a novel PP1-interacting protein that mediates human plasma's anti-apoptotic effect in endothelial cells.
Journal of cellular and molecular medicine21(9)2068-2076(2017 Sep)
Yao H,He G,Chen C,Yan S,Lu L,Song L,Vijayan KV,Li Q,Xiong L,Miao X,Deng X

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Systematic Analysis of Human Protein Phosphatase Interactions and Dynamics.
Cell systems4(4)430-444.e5(2017 Apr)
Yadav L,Tamene F,G??s H,van Drogen A,Katainen R,Aebersold R,Gstaiger M,Varjosalo M

The recurrent PPP1CB mutation p.Pro49Arg in an additional Noonan-like syndrome individual: Broadening the clinical phenotype.
American journal of medical genetics. Part A173(3)824-828(2017 Mar)
Bertola D,Yamamoto G,Buscarilli M,Jorge A,Passos-Bueno MR,Kim C

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PPP1CB cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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