PLAAT2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLAAT2
Entrez Gene ID	54979
Full Name	HRAS like suppressor 2
Synonyms	PLA1/2-2
General protein information	Preferred Names HRAS like suppressor 2 Names HRAS-like suppressor 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q12.3
Summary	The protein encoded by this gene has both phospholipase and acyltransferase activities and acts as a tumor suppressor. The encoded protein can hydrolyze dipalmitoylated phosphatidylcholine (PC) to palmitic acid and lyso-PC. In addition, this protein can catalyze the N-acylation of phosphatidylethanolamine and can catalyze the O-acylation of lyso-PC to form PC. [provided by RefSeq, Jul 2016].
Disorder MIM:	613866

mRNA and Protein(s)

mRNA	Protein	Name
NM_017878.1	NP_060348.1	HRAS-like suppressor 2
XM_011545120.2	XP_011543422.1	phospholipase A and acyltransferase 2 isoform X1
NM_017878.2	NP_060348.1	phospholipase A and acyltransferase 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	HRASLS2	NP_060348.1
Pan troglodytes (chimpanzee)	HRASLS2	XP_508514.2
	LOC722182	XP_001118366.2

Gene Ontology
Bibliography

Related articles in PubMed

Generation of N-acylphosphatidylethanolamine by members of the phospholipase A/acyltransferase (PLA/AT) family.
Uyama T, Ikematsu N, Inoue M, Shinohara N, Jin XH, Tsuboi K, Tonai T, Tokumura A, Ueda N
The Journal of biological chemistry287(38)31905-19(2012 Sep)

Structural basis for the acyltransferase activity of lecithin:retinol acyltransferase-like proteins.
Golczak M, Kiser PD, Sears AE, Lodowski DT, Blaner WS, Palczewski K
The Journal of biological chemistry287(28)23790-807(2012 Jul)

Characterization of the human tumor suppressors TIG3 and HRASLS2 as phospholipid-metabolizing enzymes.
Uyama T, Jin XH, Tsuboi K, Tonai T, Ueda N
Biochimica et biophysica acta1791(12)1114-24(2009 Dec)

Cloning and functional characterization of the HRASLS2 gene.
Shyu RY, Hsieh YC, Tsai FM, Wu CC, Jiang SY
Amino acids35(1)129-37(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that acyl-modified forms of HRAS-like tumor suppressors HRASLS2 and HRASLS3 mimicking lipolytic activity of lecithin retinol acyltransferase LRAT.
Title: Structural basis for the acyltransferase activity of lecithin:retinol acyltransferase-like proteins.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

The tumor suppressors TIG3, HRASLS2 and H-rev107 are involved in the phospholipid metabolism with different physiological roles.
Title: Characterization of the human tumor suppressors TIG3 and HRASLS2 as phospholipid-metabolizing enzymes.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

HRASLS2 protein suppressed growth and RAS activities of cancer cells, and the C-terminal hydrophobic domain appeared to be indispensable for both activities.
Title: Cloning and functional characterization of the HRASLS2 gene.

The following PLAAT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLAAT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02604	NM_017878.2 Latest version!	Homo sapiens phospholipase A and acyltransferase 2 (PLAAT2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu68850	XM_011545120.2 Latest version!	Homo sapiens phospholipase A and acyltransferase 2 (PLAAT2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu02604	NM_017878.1	Homo sapiens HRAS like suppressor 2 (HRASLS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLAAT2 ( NM_017878.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_060348.1 Homo sapiens HRAS like suppressor 2 (HRASLS2), mRNA.

Price & Availability↑

CloneID	OHu02604
Clone ID Related Accession (Same CDS sequence)	NM_017878.1 , NM_017878.2
Accession Version	NM_017878.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	HRAS-like suppressor 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK000563.1. ##Evidence-Data-START## Transcript exon combination :: AK000563.1, ERR279873.2047.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTTGG CCAGACCAAG ACCGAGACTT GGAGACCTGA TTGAGATTTC TCGCTTTGGC 
TATGCACACT GGGCCATCTA CGTGGGAGAT GGCTATGTGG TCCATCTGGC TCCGGCAAGT 
GAAATTGCTG GAGCTGGTGC GGCCAGTGTC CTGTCTGCCC TGACCAACAA AGCCATAGTG 
AAGAAGGAAC TGCTGTCTGT GGTGGCTGGG GGAGACAACT ACAGGGTCAA TAACAAGCAC 
GATGACAGAT ACACACCACT GCCTTCCAAC AAAATCGTCA AGCGGGCAGA GGAGTTGGTG 
GGGCAGGAGT TGCCTTATTC GCTGACCAGT GACAACTGCG AGCACTTCGT GAACCATCTG 
CGCTATGGCG TCTCCCGCAG TGACCAGGTC ACTGGTGCAG TCACGACAGT AGGTGTGGCA 
GCAGGCCTGC TGGCTGCCGC AAGCCTTGTG GGGATCCTGC TGGCCAGAAG CAAGCGGGAA 
AGGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060348.1
CDS	60..548
Translation	MALARPRPRLGDLIEISRFGYAHWAIYVGDGYVVHLAPASEIAGAGAASVLSALTNKAIVKKELLSVVAGGDNYRVNNKHDDRYTPLPSNKIVKRAEELVGQELPYSLTSDNCEHFVNHLRYGVSRSDQVTGAVTTVGVAAGLLAAASLVGILLARSKRERQ

Target ORF information:

RefSeq Version	NM_017878.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HRAS like suppressor 2 (HRASLS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017878.1

ORF Insert Sequence:

ATGGCTTTGG CCAGACCAAG ACCGAGACTT GGAGACCTGA TTGAGATTTC TCGCTTTGGC 
TATGCACACT GGGCCATCTA CGTGGGAGAT GGCTATGTGG TCCATCTGGC TCCGGCAAGT 
GAAATTGCTG GAGCTGGTGC GGCCAGTGTC CTGTCTGCCC TGACCAACAA AGCCATAGTG 
AAGAAGGAAC TGCTGTCTGT GGTGGCTGGG GGAGACAACT ACAGGGTCAA TAACAAGCAC 
GATGACAGAT ACACACCACT GCCTTCCAAC AAAATCGTCA AGCGGGCAGA GGAGTTGGTG 
GGGCAGGAGT TGCCTTATTC GCTGACCAGT GACAACTGCG AGCACTTCGT GAACCATCTG 
CGCTATGGCG TCTCCCGCAG TGACCAGGTC ACTGGTGCAG TCACGACAGT AGGTGTGGCA 
GCAGGCCTGC TGGCTGCCGC AAGCCTTGTG GGGATCCTGC TGGCCAGAAG CAAGCGGGAA 
AGGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLAAT2 ( NM_017878.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_060348.1 Homo sapiens phospholipase A and acyltransferase 2 (PLAAT2), mRNA.

Price & Availability↑

CloneID	OHu02604
Clone ID Related Accession (Same CDS sequence)	NM_017878.1 , NM_017878.2
Accession Version	NM_017878.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-08
Organism	Homo sapiens(human)
Product	phospholipase A and acyltransferase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK000563.1. On Nov 22, 2018 this sequence version replaced NM_017878.1. ##Evidence-Data-START## Transcript exon combination :: AK000563.1, ERR279873.2047.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000255695.2/ ENSP00000255695.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTTTGG CCAGACCAAG ACCGAGACTT GGAGACCTGA TTGAGATTTC TCGCTTTGGC 
TATGCACACT GGGCCATCTA CGTGGGAGAT GGCTATGTGG TCCATCTGGC TCCGGCAAGT 
GAAATTGCTG GAGCTGGTGC GGCCAGTGTC CTGTCTGCCC TGACCAACAA AGCCATAGTG 
AAGAAGGAAC TGCTGTCTGT GGTGGCTGGG GGAGACAACT ACAGGGTCAA TAACAAGCAC 
GATGACAGAT ACACACCACT GCCTTCCAAC AAAATCGTCA AGCGGGCAGA GGAGTTGGTG 
GGGCAGGAGT TGCCTTATTC GCTGACCAGT GACAACTGCG AGCACTTCGT GAACCATCTG 
CGCTATGGCG TCTCCCGCAG TGACCAGGTC ACTGGTGCAG TCACGACAGT AGGTGTGGCA 
GCAGGCCTGC TGGCTGCCGC AAGCCTTGTG GGGATCCTGC TGGCCAGAAG CAAGCGGGAA 
AGGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060348.1
CDS	56..544
Translation	MALARPRPRLGDLIEISRFGYAHWAIYVGDGYVVHLAPASEIAGAGAASVLSALTNKAIVKKELLSVVAGGDNYRVNNKHDDRYTPLPSNKIVKRAEELVGQELPYSLTSDNCEHFVNHLRYGVSRSDQVTGAVTTVGVAAGLLAAASLVGILLARSKRERQ

Target ORF information:

RefSeq Version	NM_017878.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase A and acyltransferase 2 (PLAAT2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017878.2

ORF Insert Sequence:

ATGGCTTTGG CCAGACCAAG ACCGAGACTT GGAGACCTGA TTGAGATTTC TCGCTTTGGC 
TATGCACACT GGGCCATCTA CGTGGGAGAT GGCTATGTGG TCCATCTGGC TCCGGCAAGT 
GAAATTGCTG GAGCTGGTGC GGCCAGTGTC CTGTCTGCCC TGACCAACAA AGCCATAGTG 
AAGAAGGAAC TGCTGTCTGT GGTGGCTGGG GGAGACAACT ACAGGGTCAA TAACAAGCAC 
GATGACAGAT ACACACCACT GCCTTCCAAC AAAATCGTCA AGCGGGCAGA GGAGTTGGTG 
GGGCAGGAGT TGCCTTATTC GCTGACCAGT GACAACTGCG AGCACTTCGT GAACCATCTG 
CGCTATGGCG TCTCCCGCAG TGACCAGGTC ACTGGTGCAG TCACGACAGT AGGTGTGGCA 
GCAGGCCTGC TGGCTGCCGC AAGCCTTGTG GGGATCCTGC TGGCCAGAAG CAAGCGGGAA 
AGGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLAAT2 ( XM_011545120.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543422.1 Homo sapiens phospholipase A and acyltransferase 2 (PLAAT2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu68850
Clone ID Related Accession (Same CDS sequence)	XM_011545120.2
Accession Version	XM_011545120.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	phospholipase A and acyltransferase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545120.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAACT GCTGTGGCTC CCAAAGAGTT TTCCAGTTCT GGCTCCAGTC CATCTGCATC 
CCCACACTAA AGCTCTTTGT TTGTTTGTTT GTTTATAGCC TGAAGAGGGC CAGACCAAGA 
CCGAGACTTG GAGACCTGAT TGAGATTTCT CGCTTTGGCT ATGCACACTG GGCCATCTAC 
GTGGGAGATG GCTATGTGGT CCATCTGGCT CCGGCAAGTG AAATTGCTGG AGCTGGTGCG 
GCCAGTGTCC TGTCTGCCCT GACCAACAAA GCCATAGTGA AGAAGGAACT GCTGTCTGTG 
GTGGCTGGGG GAGACAACTA CAGGGTCAAT AACAAGCACG ATGACAGATA CACACCACTG 
CCTTCCAACA AAATCGTCAA GCGGGCAGAG GAGTTGGTGG GGCAGGAGTT GCCTTATTCG 
CTGACCAGTG ACAACTGCGA GCACTTCGTG AACCATCTGC GCTATGGCGT CTCCCGCAGT 
GACCAGGTCA CTGGTGCAGT CACGACAGTA GGTGTGGCAG CAGGCCTGCT GGCTGCCGCA 
AGCCTTGTGG GGATCCTGCT GGCCAGAAGC AAGCGGGAAA GGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543422.1
CDS	413..1000
Translation	MQNCCGSQRVFQFWLQSICIPTLKLFVCLFVYSLKRARPRPRLGDLIEISRFGYAHWAIYVGDGYVVHLAPASEIAGAGAASVLSALTNKAIVKKELLSVVAGGDNYRVNNKHDDRYTPLPSNKIVKRAEELVGQELPYSLTSDNCEHFVNHLRYGVSRSDQVTGAVTTVGVAAGLLAAASLVGILLARSKRERQ

Target ORF information:

RefSeq Version	XM_011545120.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase A and acyltransferase 2 (PLAAT2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545120.2

ORF Insert Sequence:

ATGCAGAACT GCTGTGGCTC CCAAAGAGTT TTCCAGTTCT GGCTCCAGTC CATCTGCATC 
CCCACACTAA AGCTCTTTGT TTGTTTGTTT GTTTATAGCC TGAAGAGGGC CAGACCAAGA 
CCGAGACTTG GAGACCTGAT TGAGATTTCT CGCTTTGGCT ATGCACACTG GGCCATCTAC 
GTGGGAGATG GCTATGTGGT CCATCTGGCT CCGGCAAGTG AAATTGCTGG AGCTGGTGCG 
GCCAGTGTCC TGTCTGCCCT GACCAACAAA GCCATAGTGA AGAAGGAACT GCTGTCTGTG 
GTGGCTGGGG GAGACAACTA CAGGGTCAAT AACAAGCACG ATGACAGATA CACACCACTG 
CCTTCCAACA AAATCGTCAA GCGGGCAGAG GAGTTGGTGG GGCAGGAGTT GCCTTATTCG 
CTGACCAGTG ACAACTGCGA GCACTTCGTG AACCATCTGC GCTATGGCGT CTCCCGCAGT 
GACCAGGTCA CTGGTGCAGT CACGACAGTA GGTGTGGCAG CAGGCCTGCT GGCTGCCGCA 
AGCCTTGTGG GGATCCTGCT GGCCAGAAGC AAGCGGGAAA GGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Generation of N-acylphosphatidylethanolamine by members of the phospholipase A/acyltransferase (PLA/AT) family.
The Journal of biological chemistry287(38)31905-19(2012 Sep)
Uyama T,Ikematsu N,Inoue M,Shinohara N,Jin XH,Tsuboi K,Tonai T,Tokumura A,Ueda N

Structural basis for the acyltransferase activity of lecithin:retinol acyltransferase-like proteins.
The Journal of biological chemistry287(28)23790-807(2012 Jul)
Golczak M,Kiser PD,Sears AE,Lodowski DT,Blaner WS,Palczewski K

Characterization of the human tumor suppressors TIG3 and HRASLS2 as phospholipid-metabolizing enzymes.
Biochimica et biophysica acta1791(12)1114-24(2009 Dec)
Uyama T,Jin XH,Tsuboi K,Tonai T,Ueda N

Cloning and functional characterization of the HRASLS2 gene.
Amino acids35(1)129-37(2008 Jun)
Shyu RY,Hsieh YC,Tsai FM,Wu CC,Jiang SY

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PLAAT2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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