IMPAD1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	IMPAD1
Entrez Gene ID	54928
Full Name	inositol monophosphatase domain containing 1
Synonyms	GPAPP,IMP 3,IMP-3,IMPA3
General protein information	Preferred Names inositol monophosphatase domain containing 1 Names inositol monophosphatase 3 Golgi 3-prime phosphoadenosine 5-prime phosphate 3-prime phosphatase IMPase 3 golgi-resident PAP phosphatase golgi-resident nucleotide phosphatase inositol monophosphatase domain-containing protein 1 inositol-1(or 4)-monophosphatase 3 myo-inositol monophosphatase A3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8q12.1
Summary	This gene encodes a member of the inositol monophosphatase family. The encoded protein is localized to the Golgi apparatus and catalyzes the hydrolysis of phosphoadenosine phosphate (PAP) to adenosine monophosphate (AMP). Mutations in this gene are a cause of GRAPP type chondrodysplasia with joint dislocations, and a pseudogene of this gene is located on the long arm of chromosome 1. [provided by RefSeq, Dec 2011].
Disorder MIM:	614010
Disorder Html:	Chondrodysplasia with joint dislocations, GPAPP type, 614078 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_017813.4	NP_060283.3	inositol monophosphatase 3
NM_017813.5	NP_060283.3	Golgi-resident adenosine 3',5'-bisphosphate 3'-phosphatase

Pathways from BioSystems

Homologies

Bos taurus (cattle)	IMPAD1	NP_001039800.1
Caenorhabditis elegans (roundworm)	CELE_Y6B3B.5	NP_001021802.1
Gallus gallus (chicken)	IMPAD1	XP_419214.3
Danio rerio (zebrafish)	impad1	NP_001032657.1
Drosophila melanogaster (fruit fly)	CG15743	NP_001259511.1
Homo sapiens (human)	IMPAD1	NP_060283.3
	IMPAD1	XP_001086527.1
Canis lupus familiaris (dog)	IMPAD1	XP_535082.1
Rattus norvegicus (Norway rat)	Impad1	NP_001008772.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001396	XP_321739.4
Xenopus tropicalis (tropical clawed frog)	impad1	NP_001016215.2
Pan troglodytes (chimpanzee)	IMPAD1	XP_519770.2
Mus musculus (house mouse)	Impad1	NP_808398.1
Rattus norvegicus (Norway rat)	LOC100910881	XP_003751783.1

Gene Ontology
Bibliography

Related articles in PubMed

IMPAD1 mutations in two Catel-Manzke like patients.
Nizon M, Alanay Y, Tuysuz B, Kiper PO, Genevi?ve D, Sillence D, Huber C, Munnich A, Cormier-Daire V
American journal of medical genetics. Part A158A(9)2183-7(2012 Sep)

Chondrodysplasia and abnormal joint development associated with mutations in IMPAD1, encoding the Golgi-resident nucleotide phosphatase, gPAPP.
Vissers LE, Lausch E, Unger S, Campos-Xavier AB, Gilissen C, Rossi A, Del Rosario M, Venselaar H, Knoll U, Nampoothiri S, Nair M, Spranger J, Brunner HG, Bonaf? L, Veltman JA, Zabel B, Superti-Furga A
American journal of human genetics88(5)608-15(2011 May)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

A role for a lithium-inhibited Golgi nucleotidase in skeletal development and sulfation.
Frederick JP, Tafari AT, Wu SM, Megosh LC, Chiou ST, Irving RP, York JD
Proceedings of the National Academy of Sciences of the United States of America105(33)11605-12(2008 Aug)

Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry.
Zhang H, Li XJ, Martin DB, Aebersold R
Nature biotechnology21(6)660-6(2003 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

concluded that IMPAD1 should be screened for patients with Catel-Manzke and additional features
Title: IMPAD1 mutations in two Catel-Manzke like patients.

Affected individuals carried homozygous missense mutations in IMPAD1, the gene coding for gPAPP, a Golgi-resident nucleotide phosphatase that hydrolyzes phosphoadenosine phosphate (PAP), the byproduct of sulfotransferase reactions, to AMP.
Title: Chondrodysplasia and abnormal joint development associated with mutations in IMPAD1, encoding the Golgi-resident nucleotide phosphatase, gPAPP.

The following IMPAD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IMPAD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22296	NM_017813.5 Latest version!	Homo sapiens inositol monophosphatase domain containing 1 (IMPAD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu22296	NM_017813.4	Homo sapiens inositol monophosphatase domain containing 1 (IMPAD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IMPAD1 ( NM_017813.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060283.3 Homo sapiens inositol monophosphatase domain containing 1 (IMPAD1), mRNA.

Price & Availability↑

CloneID	OHu22296
Clone ID Related Accession (Same CDS sequence)	NM_017813.4 , NM_017813.5
Accession Version	NM_017813.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	inositol monophosphatase 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI601447.1, BC017797.1, AC032027.7 and AI095394.1. This sequence is a reference standard in the RefSeqGene project. On Dec 15, 2011 this sequence version replaced NM_017813.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.63913.1, SRR1803615.21997.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCCCA TGGGCATCCG CCTTTCCCCA CTGGGGGTGG CAGTGTTTTG CCTGCTGGGG 
CTCGGCGTGC TCTACCACCT CTACTCGGGC TTCTTGGCCG GCCGCTTCAG CCTCTTCGGC 
CTGGGCGGCG AGCCTGGCGG CGGCGCGGCG GGGCCCGCGG CCGCGGCCGA TGGGGGCACC 
GTGGACTTGC GCGAGATGCT GGCTGTGTCA GTGCTGGCCG CAGTCCGCGG CGGCGACGAG 
GTGAGGCGCG TCCGCGAGAG CAACGTCCTC CACGAGAAGT CCAAGGGGAA GACGCGCGAG 
GGAGCCGAGG ACAAGATGAC CAGCGGCGAC GTGCTGTCCA ACCGCAAGAT GTTCTACCTG 
CTCAAGACCG CCTTCCCCAG CGTCCAGATT AATACTGAGG AACACGTGGA TGCAGCTGAT 
CAGGAGGTTA TCTTGTGGGA TCATAAGATT CCTGAGGATA TCCTAAAGGA AGTAACTACT 
CCTAAAGAGG TACCAGCAGA AAGTGTTACT GTCTGGATTG ACCCACTTGA TGCTACACAG 
GAATATACAG AGGATCTTCG AAAGTACGTC ACTACTATGG TGTGTGTGGC TGTAAATGGT 
AAACCCATGC TAGGAGTTAT ACATAAGCCA TTTTCCGAAT ATACAGCTTG GGCAATGGTA 
GATGGTGGTT CAAATGTGAA AGCCCGCTCT TCCTACAATG AGAAGACCCC AAGGATCGTT 
GTGTCTCGTT CCCATTCAGG GATGGTCAAA CAGGTCGCTC TTCAGACTTT TGGAAACCAG 
ACTACAATTA TCCCAGCTGG TGGTGCTGGT TATAAAGTTT TAGCACTTTT GGATGTGCCT 
GATAAGAGTC AAGAAAAAGC TGATTTATAC ATCCATGTGA CATACATCAA AAAGTGGGAT 
ATATGTGCTG GTAATGCCAT CTTAAAAGCC CTAGGGGGGC ATATGACTAC CCTGAGTGGT 
GAAGAAATCA GTTACACTGG TTCAGACGGC ATTGAAGGGG GACTCCTTGC TAGCATCAGA 
ATGAACCACC AGGCCCTGGT CAGAAAACTC CCAGATCTAG AAAAGACAGG ACATAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060283.3
CDS	287..1366
Translation	MAPMGIRLSPLGVAVFCLLGLGVLYHLYSGFLAGRFSLFGLGGEPGGGAAGPAAAADGGTVDLREMLAVSVLAAVRGGDEVRRVRESNVLHEKSKGKTREGAEDKMTSGDVLSNRKMFYLLKTAFPSVQINTEEHVDAADQEVILWDHKIPEDILKEVTTPKEVPAESVTVWIDPLDATQEYTEDLRKYVTTMVCVAVNGKPMLGVIHKPFSEYTAWAMVDGGSNVKARSSYNEKTPRIVVSRSHSGMVKQVALQTFGNQTTIIPAGGAGYKVLALLDVPDKSQEKADLYIHVTYIKKWDICAGNAILKALGGHMTTLSGEEISYTGSDGIEGGLLASIRMNHQALVRKLPDLEKTGHK

Target ORF information:

RefSeq Version	NM_017813.4
Organism	Homo sapiens(human)
Definition	Homo sapiens inositol monophosphatase domain containing 1 (IMPAD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017813.4

ORF Insert Sequence:

ATGGCCCCCA TGGGCATCCG CCTTTCCCCA CTGGGGGTGG CAGTGTTTTG CCTGCTGGGG 
CTCGGCGTGC TCTACCACCT CTACTCGGGC TTCTTGGCCG GCCGCTTCAG CCTCTTCGGC 
CTGGGCGGCG AGCCTGGCGG CGGCGCGGCG GGGCCCGCGG CCGCGGCCGA TGGGGGCACC 
GTGGACTTGC GCGAGATGCT GGCTGTGTCA GTGCTGGCCG CAGTCCGCGG CGGCGACGAG 
GTGAGGCGCG TCCGCGAGAG CAACGTCCTC CACGAGAAGT CCAAGGGGAA GACGCGCGAG 
GGAGCCGAGG ACAAGATGAC CAGCGGCGAC GTGCTGTCCA ACCGCAAGAT GTTCTACCTG 
CTCAAGACCG CCTTCCCCAG CGTCCAGATT AATACTGAGG AACACGTGGA TGCAGCTGAT 
CAGGAGGTTA TCTTGTGGGA TCATAAGATT CCTGAGGATA TCCTAAAGGA AGTAACTACT 
CCTAAAGAGG TACCAGCAGA AAGTGTTACT GTCTGGATTG ACCCACTTGA TGCTACACAG 
GAATATACAG AGGATCTTCG AAAGTACGTC ACTACTATGG TGTGTGTGGC TGTAAATGGT 
AAACCCATGC TAGGAGTTAT ACATAAGCCA TTTTCCGAAT ATACAGCTTG GGCAATGGTA 
GATGGTGGTT CAAATGTGAA AGCCCGCTCT TCCTACAATG AGAAGACCCC AAGGATCGTT 
GTGTCTCGTT CCCATTCAGG GATGGTCAAA CAGGTCGCTC TTCAGACTTT TGGAAACCAG 
ACTACAATTA TCCCAGCTGG TGGTGCTGGT TATAAAGTTT TAGCACTTTT GGATGTGCCT 
GATAAGAGTC AAGAAAAAGC TGATTTATAC ATCCATGTGA CATACATCAA AAAGTGGGAT 
ATATGTGCTG GTAATGCCAT CTTAAAAGCC CTAGGGGGGC ATATGACTAC CCTGAGTGGT 
GAAGAAATCA GTTACACTGG TTCAGACGGC ATTGAAGGGG GACTCCTTGC TAGCATCAGA 
ATGAACCACC AGGCCCTGGT CAGAAAACTC CCAGATCTAG AAAAGACAGG ACATAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IMPAD1 ( NM_017813.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060283.3 Homo sapiens inositol monophosphatase domain containing 1 (IMPAD1), mRNA.

Price & Availability↑

CloneID	OHu22296
Clone ID Related Accession (Same CDS sequence)	NM_017813.4 , NM_017813.5
Accession Version	NM_017813.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-23
Organism	Homo sapiens(human)
Product	Golgi-resident adenosine 3',5'-bisphosphate 3'-phosphatase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI601447.1, BC017797.1, AC032027.7 and AI095394.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_017813.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.63913.1, SRR1803615.21997.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000262644.9/ ENSP00000262644.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCCCCA TGGGCATCCG CCTTTCCCCA CTGGGGGTGG CAGTGTTTTG CCTGCTGGGG 
CTCGGCGTGC TCTACCACCT CTACTCGGGC TTCTTGGCCG GCCGCTTCAG CCTCTTCGGC 
CTGGGCGGCG AGCCTGGCGG CGGCGCGGCG GGGCCCGCGG CCGCGGCCGA TGGGGGCACC 
GTGGACTTGC GCGAGATGCT GGCTGTGTCA GTGCTGGCCG CAGTCCGCGG CGGCGACGAG 
GTGAGGCGCG TCCGCGAGAG CAACGTCCTC CACGAGAAGT CCAAGGGGAA GACGCGCGAG 
GGAGCCGAGG ACAAGATGAC CAGCGGCGAC GTGCTGTCCA ACCGCAAGAT GTTCTACCTG 
CTCAAGACCG CCTTCCCCAG CGTCCAGATT AATACTGAGG AACACGTGGA TGCAGCTGAT 
CAGGAGGTTA TCTTGTGGGA TCATAAGATT CCTGAGGATA TCCTAAAGGA AGTAACTACT 
CCTAAAGAGG TACCAGCAGA AAGTGTTACT GTCTGGATTG ACCCACTTGA TGCTACACAG 
GAATATACAG AGGATCTTCG AAAGTACGTC ACTACTATGG TGTGTGTGGC TGTAAATGGT 
AAACCCATGC TAGGAGTTAT ACATAAGCCA TTTTCCGAAT ATACAGCTTG GGCAATGGTA 
GATGGTGGTT CAAATGTGAA AGCCCGCTCT TCCTACAATG AGAAGACCCC AAGGATCGTT 
GTGTCTCGTT CCCATTCAGG GATGGTCAAA CAGGTCGCTC TTCAGACTTT TGGAAACCAG 
ACTACAATTA TCCCAGCTGG TGGTGCTGGT TATAAAGTTT TAGCACTTTT GGATGTGCCT 
GATAAGAGTC AAGAAAAAGC TGATTTATAC ATCCATGTGA CATACATCAA AAAGTGGGAT 
ATATGTGCTG GTAATGCCAT CTTAAAAGCC CTAGGGGGGC ATATGACTAC CCTGAGTGGT 
GAAGAAATCA GTTACACTGG TTCAGACGGC ATTGAAGGGG GACTCCTTGC TAGCATCAGA 
ATGAACCACC AGGCCCTGGT CAGAAAACTC CCAGATCTAG AAAAGACAGG ACATAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060283.3
CDS	283..1362
Translation	MAPMGIRLSPLGVAVFCLLGLGVLYHLYSGFLAGRFSLFGLGGEPGGGAAGPAAAADGGTVDLREMLAVSVLAAVRGGDEVRRVRESNVLHEKSKGKTREGAEDKMTSGDVLSNRKMFYLLKTAFPSVQINTEEHVDAADQEVILWDHKIPEDILKEVTTPKEVPAESVTVWIDPLDATQEYTEDLRKYVTTMVCVAVNGKPMLGVIHKPFSEYTAWAMVDGGSNVKARSSYNEKTPRIVVSRSHSGMVKQVALQTFGNQTTIIPAGGAGYKVLALLDVPDKSQEKADLYIHVTYIKKWDICAGNAILKALGGHMTTLSGEEISYTGSDGIEGGLLASIRMNHQALVRKLPDLEKTGHK

Target ORF information:

RefSeq Version	NM_017813.5
Organism	Homo sapiens(human)
Definition	Homo sapiens inositol monophosphatase domain containing 1 (IMPAD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017813.5

ORF Insert Sequence:

ATGGCCCCCA TGGGCATCCG CCTTTCCCCA CTGGGGGTGG CAGTGTTTTG CCTGCTGGGG 
CTCGGCGTGC TCTACCACCT CTACTCGGGC TTCTTGGCCG GCCGCTTCAG CCTCTTCGGC 
CTGGGCGGCG AGCCTGGCGG CGGCGCGGCG GGGCCCGCGG CCGCGGCCGA TGGGGGCACC 
GTGGACTTGC GCGAGATGCT GGCTGTGTCA GTGCTGGCCG CAGTCCGCGG CGGCGACGAG 
GTGAGGCGCG TCCGCGAGAG CAACGTCCTC CACGAGAAGT CCAAGGGGAA GACGCGCGAG 
GGAGCCGAGG ACAAGATGAC CAGCGGCGAC GTGCTGTCCA ACCGCAAGAT GTTCTACCTG 
CTCAAGACCG CCTTCCCCAG CGTCCAGATT AATACTGAGG AACACGTGGA TGCAGCTGAT 
CAGGAGGTTA TCTTGTGGGA TCATAAGATT CCTGAGGATA TCCTAAAGGA AGTAACTACT 
CCTAAAGAGG TACCAGCAGA AAGTGTTACT GTCTGGATTG ACCCACTTGA TGCTACACAG 
GAATATACAG AGGATCTTCG AAAGTACGTC ACTACTATGG TGTGTGTGGC TGTAAATGGT 
AAACCCATGC TAGGAGTTAT ACATAAGCCA TTTTCCGAAT ATACAGCTTG GGCAATGGTA 
GATGGTGGTT CAAATGTGAA AGCCCGCTCT TCCTACAATG AGAAGACCCC AAGGATCGTT 
GTGTCTCGTT CCCATTCAGG GATGGTCAAA CAGGTCGCTC TTCAGACTTT TGGAAACCAG 
ACTACAATTA TCCCAGCTGG TGGTGCTGGT TATAAAGTTT TAGCACTTTT GGATGTGCCT 
GATAAGAGTC AAGAAAAAGC TGATTTATAC ATCCATGTGA CATACATCAA AAAGTGGGAT 
ATATGTGCTG GTAATGCCAT CTTAAAAGCC CTAGGGGGGC ATATGACTAC CCTGAGTGGT 
GAAGAAATCA GTTACACTGG TTCAGACGGC ATTGAAGGGG GACTCCTTGC TAGCATCAGA 
ATGAACCACC AGGCCCTGGT CAGAAAACTC CCAGATCTAG AAAAGACAGG ACATAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

IMPAD1 mutations in two Catel-Manzke like patients.
American journal of medical genetics. Part A158A(9)2183-7(2012 Sep)
Nizon M,Alanay Y,Tuysuz B,Kiper PO,Genevi?ve D,Sillence D,Huber C,Munnich A,Cormier-Daire V

Chondrodysplasia and abnormal joint development associated with mutations in IMPAD1, encoding the Golgi-resident nucleotide phosphatase, gPAPP.
American journal of human genetics88(5)608-15(2011 May)
Vissers LE,Lausch E,Unger S,Campos-Xavier AB,Gilissen C,Rossi A,Del Rosario M,Venselaar H,Knoll U,Nampoothiri S,Nair M,Spranger J,Brunner HG,Bonaf? L,Veltman JA,Zabel B,Superti-Furga A

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

A role for a lithium-inhibited Golgi nucleotidase in skeletal development and sulfation.
Proceedings of the National Academy of Sciences of the United States of America105(33)11605-12(2008 Aug)
Frederick JP,Tafari AT,Wu SM,Megosh LC,Chiou ST,Irving RP,York JD

Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry.
Nature biotechnology21(6)660-6(2003 Jun)
Zhang H,Li XJ,Martin DB,Aebersold R

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IMPAD1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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