ORF » Species Summary » Homo sapiens » LIME1 cDNA ORF clone

LIME1 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following LIME1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIME1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu13372 NM_017806.4
Latest version!		 Homo sapiens Lck interacting transmembrane adaptor 1 (LIME1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$154.50-$216.30
$309.00
OHu68791 NM_001305655.2
Latest version!		 Homo sapiens Lck interacting transmembrane adaptor 1 (LIME1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu68791 NM_001305654.1
Latest version!		 Homo sapiens Lck interacting transmembrane adaptor 1 (LIME1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
View Old Accession Versions >>
OHu68791 NM_001305655.1 Homo sapiens Lck interacting transmembrane adaptor 1 (LIME1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu13372 NM_017806.3 Homo sapiens Lck interacting transmembrane adaptor 1 (LIME1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$154.50-$216.30
$309.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LIME1 ( NM_017806.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060276.2 Homo sapiens Lck interacting transmembrane adaptor 1 (LIME1), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu13372
    Clone ID Related Accession (Same CDS sequence) NM_017806.4 , NM_017806.3
    Accession Version NM_017806.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-01
    Organism Homo sapiens(human)
    Product lck-interacting transmembrane adapter 1 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK000413.1 and AL121845.20. On Nov 22, 2018 this sequence version replaced NM_017806.3. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK000413.1, SRR1163655.157676.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000309546.8/ ENSP00000309521.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGGGCTGC CAGTGTCCTG GGCCCCTCCT GCCCTCTGGG TTCTAGGGTG CTGCGCCCTG 
    CTCCTCTCGC TGTGGGCGCT GTGCACAGCC TGCCGCAGGC CCGAGGACGC TGTAGCCCCC 
    AGGAAGAGGG CGCGGAGGCA GCGGGCGAGG CTGCAGGGCA GTGCGACGGC GGCGGAAGCG 
    TCCCTACTGA GGCGGACCCA CCTCTGCTCC CTCAGCAAGT CGGACACCAG ACTGCACGAG 
    CTGCACCGGG GCCCGCGCAG CAGCAGGGCC CTGCGGCCTG CCAGCATGGA TCTCCTGCGC 
    CCACACTGGC TGGAGGTGTC CAGGGACATC ACCGGACCGC AGGCAGCCCC CTCTGCCTTC 
    CCACACCAGG AGCTGCCCCG GGCTCTGCCG GCAGCTGCAG CCACCGCAGG GTGCGCTGGC 
    CTCGAGGCCA CCTATTCCAA CGTGGGGCTG GCGGCCCTTC CCGGGGTCAG CCTGGCGGCC 
    AGCCCTGTGG TGGCCGAGTA TGCCCGCGTC CAGAAGCGCA AAGGGACCCA TCGCAGTCCC 
    CAAGAGCCAC AGCAGGGGAA GACTGAGGTG ACCCCGGCCG CTCAGGTGGA CGTCCTGTAC 
    TCCAGGGTCT GCAAGCCTAA AAGGAGGGAC CCAGGACCCA CCACAGACCC GCTGGACCCC 
    AAGGGCCAGG GAGCGATTCT GGCCCTGGCG GGTGACCTGG CCTACCAGAC CCTCCCGCTC 
    AGGGCCCTGG ATGTGGACAG CGGCCCCCTG GAAAACGTGT ATGAGAGCAT CCGGGAGCTG 
    GGGGACCCTG CTGGCAGGAG CAGCACGTGC GGGGCTGGGA CGCCCCCTGC TTCCAGCTGC 
    CCCAGCCTAG GGAGGGGCTG GAGACCCCTC CCTGCCTCCC TGCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060276.2
    CDS67..954
    Translation

    Target ORF information:

    RefSeq Version NM_017806.4
    Organism Homo sapiens(human)
    Definition Homo sapiens Lck interacting transmembrane adaptor 1 (LIME1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_017806.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGGGCTGC CAGTGTCCTG GGCCCCTCCT GCCCTCTGGG TTCTAGGGTG CTGCGCCCTG 
    CTCCTCTCGC TGTGGGCGCT GTGCACAGCC TGCCGCAGGC CCGAGGACGC TGTAGCCCCC 
    AGGAAGAGGG CGCGGAGGCA GCGGGCGAGG CTGCAGGGCA GTGCGACGGC GGCGGAAGCG 
    TCCCTACTGA GGCGGACCCA CCTCTGCTCC CTCAGCAAGT CGGACACCAG ACTGCACGAG 
    CTGCACCGGG GCCCGCGCAG CAGCAGGGCC CTGCGGCCTG CCAGCATGGA TCTCCTGCGC 
    CCACACTGGC TGGAGGTGTC CAGGGACATC ACCGGACCGC AGGCAGCCCC CTCTGCCTTC 
    CCACACCAGG AGCTGCCCCG GGCTCTGCCG GCAGCTGCAG CCACCGCAGG GTGCGCTGGC 
    CTCGAGGCCA CCTATTCCAA CGTGGGGCTG GCGGCCCTTC CCGGGGTCAG CCTGGCGGCC 
    AGCCCTGTGG TGGCCGAGTA TGCCCGCGTC CAGAAGCGCA AAGGGACCCA TCGCAGTCCC 
    CAAGAGCCAC AGCAGGGGAA GACTGAGGTG ACCCCGGCCG CTCAGGTGGA CGTCCTGTAC 
    TCCAGGGTCT GCAAGCCTAA AAGGAGGGAC CCAGGACCCA CCACAGACCC GCTGGACCCC 
    AAGGGCCAGG GAGCGATTCT GGCCCTGGCG GGTGACCTGG CCTACCAGAC CCTCCCGCTC 
    AGGGCCCTGG ATGTGGACAG CGGCCCCCTG GAAAACGTGT ATGAGAGCAT CCGGGAGCTG 
    GGGGACCCTG CTGGCAGGAG CAGCACGTGC GGGGCTGGGA CGCCCCCTGC TTCCAGCTGC 
    CCCAGCCTAG GGAGGGGCTG GAGACCCCTC CCTGCCTCCC TGCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LIME1 ( NM_017806.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060276.2 Homo sapiens Lck interacting transmembrane adaptor 1 (LIME1), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu13372
    Clone ID Related Accession (Same CDS sequence) NM_017806.4 , NM_017806.3
    Accession Version NM_017806.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-02
    Organism Homo sapiens(human)
    Product lck-interacting transmembrane adapter 1 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI911934.1, AL121845.20, AK000413.1 and AI763397.1. On Mar 27, 2015 this sequence version replaced NM_017806.2. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1163655.54508.1, AK000413.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGGGCTGC CAGTGTCCTG GGCCCCTCCT GCCCTCTGGG TTCTAGGGTG CTGCGCCCTG 
    CTCCTCTCGC TGTGGGCGCT GTGCACAGCC TGCCGCAGGC CCGAGGACGC TGTAGCCCCC 
    AGGAAGAGGG CGCGGAGGCA GCGGGCGAGG CTGCAGGGCA GTGCGACGGC GGCGGAAGCG 
    TCCCTACTGA GGCGGACCCA CCTCTGCTCC CTCAGCAAGT CGGACACCAG ACTGCACGAG 
    CTGCACCGGG GCCCGCGCAG CAGCAGGGCC CTGCGGCCTG CCAGCATGGA TCTCCTGCGC 
    CCACACTGGC TGGAGGTGTC CAGGGACATC ACCGGACCGC AGGCAGCCCC CTCTGCCTTC 
    CCACACCAGG AGCTGCCCCG GGCTCTGCCG GCAGCTGCAG CCACCGCAGG GTGCGCTGGC 
    CTCGAGGCCA CCTATTCCAA CGTGGGGCTG GCGGCCCTTC CCGGGGTCAG CCTGGCGGCC 
    AGCCCTGTGG TGGCCGAGTA TGCCCGCGTC CAGAAGCGCA AAGGGACCCA TCGCAGTCCC 
    CAAGAGCCAC AGCAGGGGAA GACTGAGGTG ACCCCGGCCG CTCAGGTGGA CGTCCTGTAC 
    TCCAGGGTCT GCAAGCCTAA AAGGAGGGAC CCAGGACCCA CCACAGACCC GCTGGACCCC 
    AAGGGCCAGG GAGCGATTCT GGCCCTGGCG GGTGACCTGG CCTACCAGAC CCTCCCGCTC 
    AGGGCCCTGG ATGTGGACAG CGGCCCCCTG GAAAACGTGT ATGAGAGCAT CCGGGAGCTG 
    GGGGACCCTG CTGGCAGGAG CAGCACGTGC GGGGCTGGGA CGCCCCCTGC TTCCAGCTGC 
    CCCAGCCTAG GGAGGGGCTG GAGACCCCTC CCTGCCTCCC TGCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060276.2
    CDS271..1158
    Translation

    Target ORF information:

    RefSeq Version NM_017806.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Lck interacting transmembrane adaptor 1 (LIME1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_017806.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGGGCTGC CAGTGTCCTG GGCCCCTCCT GCCCTCTGGG TTCTAGGGTG CTGCGCCCTG 
    CTCCTCTCGC TGTGGGCGCT GTGCACAGCC TGCCGCAGGC CCGAGGACGC TGTAGCCCCC 
    AGGAAGAGGG CGCGGAGGCA GCGGGCGAGG CTGCAGGGCA GTGCGACGGC GGCGGAAGCG 
    TCCCTACTGA GGCGGACCCA CCTCTGCTCC CTCAGCAAGT CGGACACCAG ACTGCACGAG 
    CTGCACCGGG GCCCGCGCAG CAGCAGGGCC CTGCGGCCTG CCAGCATGGA TCTCCTGCGC 
    CCACACTGGC TGGAGGTGTC CAGGGACATC ACCGGACCGC AGGCAGCCCC CTCTGCCTTC 
    CCACACCAGG AGCTGCCCCG GGCTCTGCCG GCAGCTGCAG CCACCGCAGG GTGCGCTGGC 
    CTCGAGGCCA CCTATTCCAA CGTGGGGCTG GCGGCCCTTC CCGGGGTCAG CCTGGCGGCC 
    AGCCCTGTGG TGGCCGAGTA TGCCCGCGTC CAGAAGCGCA AAGGGACCCA TCGCAGTCCC 
    CAAGAGCCAC AGCAGGGGAA GACTGAGGTG ACCCCGGCCG CTCAGGTGGA CGTCCTGTAC 
    TCCAGGGTCT GCAAGCCTAA AAGGAGGGAC CCAGGACCCA CCACAGACCC GCTGGACCCC 
    AAGGGCCAGG GAGCGATTCT GGCCCTGGCG GGTGACCTGG CCTACCAGAC CCTCCCGCTC 
    AGGGCCCTGG ATGTGGACAG CGGCCCCCTG GAAAACGTGT ATGAGAGCAT CCGGGAGCTG 
    GGGGACCCTG CTGGCAGGAG CAGCACGTGC GGGGCTGGGA CGCCCCCTGC TTCCAGCTGC 
    CCCAGCCTAG GGAGGGGCTG GAGACCCCTC CCTGCCTCCC TGCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LIME1 ( NM_001305654.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001292583.1 Homo sapiens Lck interacting transmembrane adaptor 1 (LIME1), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu68791
    Clone ID Related Accession (Same CDS sequence) NM_001305654.1 , NM_001305655.2 , NM_001305655.1
    Accession Version NM_001305654.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product lck-interacting transmembrane adapter 1 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY203957.1, AL121845.20 and AI763397.1. Transcript Variant: This variant (2) contains an alternate exon in the 5' UTR and uses an alternate splice site in an internal exon resulting in a frameshift, compared to variant 1. The resulting protein (isoform 2) has a distinct C-terminus and is shorter than isoform 1. Both variants 2 and 3 encode the same protein (isoform 2). ##Evidence-Data-START## Transcript exon combination :: AY203957.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGGGCTGC CAGTGTCCTG GGCCCCTCCT GCCCTCTGGG TTCTAGGGTG CTGCGCCCTG 
    CTCCTCTCGC TGTGGGCGCT GTGCACAGCC TGCCGCAGGC CCGAGGACGC TGTAGCCCCC 
    AGGAAGAGGG CGCGGAGGCA GCGGGCGAGG CTGCAGGGCA GTGCGACGGC GGCGGAAGCG 
    CAAGTCGGAC ACCAGACTGC ACGAGCTGCA CCGGGGCCCG CGCAGCAGCA GGGCCCTGCG 
    GCCTGCCAGC ATGGATCTCC TGCGCCCACA CTGGCTGGAG GTGTCCAGGG ACATCACCGG 
    ACCGCAGGCA GCCCCCTCTG CCTTCCCACA CCAGGAGCTG CCCCGGGCTC TGCCGGCAGC 
    TGCAGCCACC GCAGGGTGCG CTGGCCTCGA GGCCACCTAT TCCAACGTGG GGCTGGCGGC 
    CCTTCCCGGG GTCAGCCTGG CGGCCAGCCC TGTGGTGGCC GAGTATGCCC GCGTCCAGAA 
    GCGCAAAGGG ACCCATCGCA GTCCCCAAGA GCCACAGCAG GGGAAGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001292583.1
    CDS504..1034
    Translation

    Target ORF information:

    RefSeq Version NM_001305654.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Lck interacting transmembrane adaptor 1 (LIME1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001305654.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGGGCTGC CAGTGTCCTG GGCCCCTCCT GCCCTCTGGG TTCTAGGGTG CTGCGCCCTG 
    CTCCTCTCGC TGTGGGCGCT GTGCACAGCC TGCCGCAGGC CCGAGGACGC TGTAGCCCCC 
    AGGAAGAGGG CGCGGAGGCA GCGGGCGAGG CTGCAGGGCA GTGCGACGGC GGCGGAAGCG 
    CAAGTCGGAC ACCAGACTGC ACGAGCTGCA CCGGGGCCCG CGCAGCAGCA GGGCCCTGCG 
    GCCTGCCAGC ATGGATCTCC TGCGCCCACA CTGGCTGGAG GTGTCCAGGG ACATCACCGG 
    ACCGCAGGCA GCCCCCTCTG CCTTCCCACA CCAGGAGCTG CCCCGGGCTC TGCCGGCAGC 
    TGCAGCCACC GCAGGGTGCG CTGGCCTCGA GGCCACCTAT TCCAACGTGG GGCTGGCGGC 
    CCTTCCCGGG GTCAGCCTGG CGGCCAGCCC TGTGGTGGCC GAGTATGCCC GCGTCCAGAA 
    GCGCAAAGGG ACCCATCGCA GTCCCCAAGA GCCACAGCAG GGGAAGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LIME1 ( NM_001305655.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001292584.1 Homo sapiens Lck interacting transmembrane adaptor 1 (LIME1), transcript variant 3, mRNA.
    Price & Availability↑
    CloneID OHu68791
    Clone ID Related Accession (Same CDS sequence) NM_001305654.1 , NM_001305655.2 , NM_001305655.1
    Accession Version NM_001305655.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-31
    Organism Homo sapiens(human)
    Product lck-interacting transmembrane adapter 1 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK000413.1 and AL121845.20. On Jun 1, 2019 this sequence version replaced NM_001305655.1. Transcript Variant: This variant (3) uses an alternate splice site in an internal exon resulting in a frameshift, compared to variant 1. The resulting protein (isoform 2) has a distinct C-terminus and is shorter than isoform 1. Both variants 2 and 3 encode the same protein (isoform 2). ##Evidence-Data-START## Transcript exon combination :: SRR1163657.467222.1, SRR1163655.711790.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGGGCTGC CAGTGTCCTG GGCCCCTCCT GCCCTCTGGG TTCTAGGGTG CTGCGCCCTG 
    CTCCTCTCGC TGTGGGCGCT GTGCACAGCC TGCCGCAGGC CCGAGGACGC TGTAGCCCCC 
    AGGAAGAGGG CGCGGAGGCA GCGGGCGAGG CTGCAGGGCA GTGCGACGGC GGCGGAAGCG 
    CAAGTCGGAC ACCAGACTGC ACGAGCTGCA CCGGGGCCCG CGCAGCAGCA GGGCCCTGCG 
    GCCTGCCAGC ATGGATCTCC TGCGCCCACA CTGGCTGGAG GTGTCCAGGG ACATCACCGG 
    ACCGCAGGCA GCCCCCTCTG CCTTCCCACA CCAGGAGCTG CCCCGGGCTC TGCCGGCAGC 
    TGCAGCCACC GCAGGGTGCG CTGGCCTCGA GGCCACCTAT TCCAACGTGG GGCTGGCGGC 
    CCTTCCCGGG GTCAGCCTGG CGGCCAGCCC TGTGGTGGCC GAGTATGCCC GCGTCCAGAA 
    GCGCAAAGGG ACCCATCGCA GTCCCCAAGA GCCACAGCAG GGGAAGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001292584.1
    CDS67..597
    Translation

    Target ORF information:

    RefSeq Version NM_001305655.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Lck interacting transmembrane adaptor 1 (LIME1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001305655.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGGGCTGC CAGTGTCCTG GGCCCCTCCT GCCCTCTGGG TTCTAGGGTG CTGCGCCCTG 
    CTCCTCTCGC TGTGGGCGCT GTGCACAGCC TGCCGCAGGC CCGAGGACGC TGTAGCCCCC 
    AGGAAGAGGG CGCGGAGGCA GCGGGCGAGG CTGCAGGGCA GTGCGACGGC GGCGGAAGCG 
    CAAGTCGGAC ACCAGACTGC ACGAGCTGCA CCGGGGCCCG CGCAGCAGCA GGGCCCTGCG 
    GCCTGCCAGC ATGGATCTCC TGCGCCCACA CTGGCTGGAG GTGTCCAGGG ACATCACCGG 
    ACCGCAGGCA GCCCCCTCTG CCTTCCCACA CCAGGAGCTG CCCCGGGCTC TGCCGGCAGC 
    TGCAGCCACC GCAGGGTGCG CTGGCCTCGA GGCCACCTAT TCCAACGTGG GGCTGGCGGC 
    CCTTCCCGGG GTCAGCCTGG CGGCCAGCCC TGTGGTGGCC GAGTATGCCC GCGTCCAGAA 
    GCGCAAAGGG ACCCATCGCA GTCCCCAAGA GCCACAGCAG GGGAAGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LIME1 ( NM_001305655.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001292584.1 Homo sapiens Lck interacting transmembrane adaptor 1 (LIME1), transcript variant 3, mRNA.
    Price & Availability↑
    CloneID OHu68791
    Clone ID Related Accession (Same CDS sequence) NM_001305654.1 , NM_001305655.2 , NM_001305655.1
    Accession Version NM_001305655.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product lck-interacting transmembrane adapter 1 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI911934.1, AL121845.20, AK000413.1 and AI763397.1. Transcript Variant: This variant (3) uses an alternate splice site in an internal exon resulting in a frameshift, compared to variant 1. The resulting protein (isoform 2) has a distinct C-terminus and is shorter than isoform 1. Both variants 2 and 3 encode the same protein (isoform 2). ##Evidence-Data-START## Transcript exon combination :: SRR1163655.211848.1, SRR1163657.467222.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGGGCTGC CAGTGTCCTG GGCCCCTCCT GCCCTCTGGG TTCTAGGGTG CTGCGCCCTG 
    CTCCTCTCGC TGTGGGCGCT GTGCACAGCC TGCCGCAGGC CCGAGGACGC TGTAGCCCCC 
    AGGAAGAGGG CGCGGAGGCA GCGGGCGAGG CTGCAGGGCA GTGCGACGGC GGCGGAAGCG 
    CAAGTCGGAC ACCAGACTGC ACGAGCTGCA CCGGGGCCCG CGCAGCAGCA GGGCCCTGCG 
    GCCTGCCAGC ATGGATCTCC TGCGCCCACA CTGGCTGGAG GTGTCCAGGG ACATCACCGG 
    ACCGCAGGCA GCCCCCTCTG CCTTCCCACA CCAGGAGCTG CCCCGGGCTC TGCCGGCAGC 
    TGCAGCCACC GCAGGGTGCG CTGGCCTCGA GGCCACCTAT TCCAACGTGG GGCTGGCGGC 
    CCTTCCCGGG GTCAGCCTGG CGGCCAGCCC TGTGGTGGCC GAGTATGCCC GCGTCCAGAA 
    GCGCAAAGGG ACCCATCGCA GTCCCCAAGA GCCACAGCAG GGGAAGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001292584.1
    CDS271..801
    Translation

    Target ORF information:

    RefSeq Version NM_001305655.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Lck interacting transmembrane adaptor 1 (LIME1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001305655.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGGGCTGC CAGTGTCCTG GGCCCCTCCT GCCCTCTGGG TTCTAGGGTG CTGCGCCCTG 
    CTCCTCTCGC TGTGGGCGCT GTGCACAGCC TGCCGCAGGC CCGAGGACGC TGTAGCCCCC 
    AGGAAGAGGG CGCGGAGGCA GCGGGCGAGG CTGCAGGGCA GTGCGACGGC GGCGGAAGCG 
    CAAGTCGGAC ACCAGACTGC ACGAGCTGCA CCGGGGCCCG CGCAGCAGCA GGGCCCTGCG 
    GCCTGCCAGC ATGGATCTCC TGCGCCCACA CTGGCTGGAG GTGTCCAGGG ACATCACCGG 
    ACCGCAGGCA GCCCCCTCTG CCTTCCCACA CCAGGAGCTG CCCCGGGCTC TGCCGGCAGC 
    TGCAGCCACC GCAGGGTGCG CTGGCCTCGA GGCCACCTAT TCCAACGTGG GGCTGGCGGC 
    CCTTCCCGGG GTCAGCCTGG CGGCCAGCCC TGTGGTGGCC GAGTATGCCC GCGTCCAGAA 
    GCGCAAAGGG ACCCATCGCA GTCCCCAAGA GCCACAGCAG GGGAAGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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