NSD3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NSD3
Entrez Gene ID	54904
Full Name	nuclear receptor binding SET domain protein 3
Synonyms	KMT3F,KMT3G,WHISTLE,WHSC1L1,pp14328
General protein information	Preferred Names nuclear receptor binding SET domain protein 3 Names histone-lysine N-methyltransferase NSD3 WHSC1-like 1 isoform 9 with methyltransferase activity to lysine Wolf-Hirschhorn syndrome candidate 1-like 1 nuclear SET domain-containing protein 3 protein whistle
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8p11.23
Summary	This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. This protein methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. Two alternatively spliced variants have been described. [provided by RefSeq, May 2015].
Disorder MIM:	607083
Disorder Html:	Leukemia, acute myeloid, 601626 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_023034.1	NP_075447.1	histone-lysine N-methyltransferase NSD3 isoform long
NM_017778.2	NP_060248.2	histone-lysine N-methyltransferase NSD3 isoform short
NM_023034.2	NP_075447.1	histone-lysine N-methyltransferase NSD3 isoform long

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	WHSC1L1	XP_004947646.1
Bos taurus (cattle)	WHSC1L1	NP_001069063.2
	WHSC1L1	XP_001091186.2
Rattus norvegicus (Norway rat)	Whsc1l1	NP_001099560.1
Pan troglodytes (chimpanzee)	WHSC1L1	XP_519713.2
Canis lupus familiaris (dog)	WHSC1L1	XP_005629910.1
Mus musculus (house mouse)	Whsc1l1	NP_001074738.1
Homo sapiens (human)	WHSC1L1	NP_075447.1
Danio rerio (zebrafish)	whsc1l1	XP_005155380.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural Mechanism of the Oxygenase JMJD6 Recognition by the Extraterminal (ET) Domain of BRD4.
Konuma T, Yu D, Zhao C, Ju Y, Sharma R, Ren C, Zhang Q, Zhou MM, Zeng L
Scientific reports7(1)16272(2017 Nov)

Silencing of histone methyltransferase NSD3 reduces cell viability in osteosarcoma with induction of apoptosis.
Liu Z, Piao L, Zhuang M, Qiu X, Xu X, Zhang D, Liu M, Ren D
Oncology reports38(5)2796-2802(2017 Nov)

Development of mammary hyperplasia, dysplasia, and invasive ductal carcinoma in transgenic mice expressing the 8p11 amplicon oncogene NSD3.
Turner-Ivey B, Smith EL, Rutkovsky AC, Spruill LS, Mills JN, Ethier SP
Breast cancer research and treatment164(2)349-358(2017 Jul)

WHSC1L1 drives cell cycle progression through transcriptional regulation of CDC6 and CDK2 in squamous cell carcinoma of the head and neck.
Saloura V, Vougiouklakis T, Zewde M, Kiyotani K, Park JH, Gao G, Karrison T, Lingen M, Nakamura Y, Hamamoto R
Oncotarget7(27)42527-42538(2016 Jul)

Amplification of WHSC1L1 regulates expression and estrogen-independent activation of ER? in SUM-44 breast cancer cells and is associated with ER? over-expression in breast cancer.
Irish JC, Mills JN, Turner-Ivey B, Wilson RC, Guest ST, Rutkovsky A, Dombkowski A, Kappler CS, Hardiman G, Ethier SP
Molecular oncology10(6)850-65(2016 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

WHSC1L1 and H3K36me2 are enriched in the gene bodies of the cell cycle-related genes CDC6 and CDK2, implying that WHSC1L1 directly regulates the transcription of these gene
Title: WHSC1L1 drives cell cycle progression through transcriptional regulation of CDC6 and CDK2 in squamous cell carcinoma of the head and neck.

This study demonstrates that over-expression of WHSC1L1 is linked to over-expression of ER-alpha in SUM-44 breast cancer cells and in primary human breast cancers.
Title: Amplification of WHSC1L1 regulates expression and estrogen-independent activation of ER? in SUM-44 breast cancer cells and is associated with ER? over-expression in breast cancer.

Results demonstrate that the AML maintenance function of BRD4 requires its interaction with the short isoform of NSD3 lacking the methyltransferase domain. This protein is an adaptor that sustains leukemia by linking BRD4 to the CHD8 chromatin remodeler.
Title: NSD3-Short Is an Adaptor Protein that Couples BRD4 to the CHD8 Chromatin Remodeler.

Studies indicate that the NSD methyltransferases NSD1, NSD2/WHSC1/MMSET and NSD3/WHSC1L1 were overexpressed, amplified or somatically mutated in multiple types of cancer, suggesting their critical role in cancer.
Title: The NSD family of protein methyltransferases in human cancer.

The results describe the binding of NSD1, 2 and 3 catalytic domains (CD) on histone tails through recognition of histone-lysine and methylation properties.
Title: In vitro histone lysine methylation by NSD1, NSD2/MMSET/WHSC1 and NSD3/WHSC1L.

The following NSD3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NSD3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02551	NM_017778.2 Latest version!	Homo sapiens nuclear receptor binding SET domain protein 3 (NSD3), transcript variant short, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu19193	NM_023034.2 Latest version!	Homo sapiens nuclear receptor binding SET domain protein 3 (NSD3), transcript variant long, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
View Old Accession Versions >>
OHu19193	NM_023034.1	Homo sapiens nuclear receptor binding SET domain protein 3 (NSD3), transcript variant long, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NSD3 ( NM_017778.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_060248.2 Homo sapiens nuclear receptor binding SET domain protein 3 (NSD3), transcript variant short, mRNA.

Price & Availability↑

CloneID	OHu02551
Clone ID Related Accession (Same CDS sequence)	NM_017778.2
Accession Version	NM_017778.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	histone-lysine N-methyltransferase NSD3 isoform short
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF332468.1. On Apr 20, 2001 this sequence version replaced NM_017778.1. Transcript Variant: This variant (short) contains an alternative 3' end sequence. The shorter protein encoded has a different carboxy terminus than the protein encoded by variant long. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF332468.1, SRR1660803.134242.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267753 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTTCT CTTTCTCTTT CATGCAAGGG ATCATGGGAA ACACAATTCA GCAACCACCT 
CAACTCATTG ACTCCGCCAA CATCCGTCAG GAGGATGCCT TTGATAACAA CAGTGACATT 
GCTGAAGATG GTGGCCAGAC ACCATATGAA GCTACTTTGC AGCAAGGCTT TCAGTACCCA 
GCTACAACAG AAGATCTTCC TCCACTCACA AATGGGTATC CATCATCAAT CAGTGTGTAT 
GAAACTCAAA CCAAATACCA GTCATATAAT CAGTATCCTA ATGGGTCAGC CAATGGCTTT 
GGTGCAGTTA GAAACTTTAG CCCCACTGAC TATTATCATT CAGAAATTCC AAACACAAGA 
CCACATGAAA TTCTGGAAAA ACCTTCCCCT CCACAGCCAC CACCTCCTCC TTCGGTACCA 
CAAACTGTGA TTCCAAAGAA GACTGGCTCA CCTGAAATTA AACTAAAAAT AACCAAAACT 
ATCCAGAATG GCAGGGAATT GTTTGAGTCT TCCCTTTGTG GAGACCTTTT AAATGAAGTA 
CAGGCAAGTG AGCACACGAA ATCAAAGCAT GAAAGCAGAA AAGAAAAGAG GAAAAAAAGC 
AACAAGCATG ACTCATCAAG ATCTGAAGAG CGCAAGTCAC ACAAAATCCC CAAATTAGAA 
CCAGAGGAAC AAAATAGACC AAATGAGAGG GTTGACACTG TATCAGAAAA ACCAAGGGAA 
GAACCAGTAC TAAAAGAGGA AGCCCCAGTT CAGCCAATAC TATCTTCTGT TCCAACAACG 
GAAGTGTCCA CTGGTGTTAA GTTTCAGGTT GGCGATCTTG TGTGGTCCAA GGTGGGAACC 
TATCCTTGGT GGCCTTGTAT GGTTTCAAGT GATCCCCAGC TTGAGGTTCA TACTAAAATT 
AACACAAGAG GTGCCCGAGA ATATCATGTC CAGTTTTTTA GCAACCAGCC AGAGAGGGCG 
TGGGTTCATG AAAAACGGGT ACGAGAGTAT AAAGGTCATA AACAGTATGA AGAATTACTG 
GCTGAGGCAA CCAAACAAGC CAGCAATCAC TCTGAGAAAC AAAAGATTCG GAAACCCCGA 
CCTCAGAGAG AACGTGCTCA GTGGGATATT GGCATTGCCC ATGCAGAGAA AGCATTGAAA 
ATGACTCGAG AAGAAAGAAT AGAACAGTAT ACTTTTATTT ACATTGATAA ACAGCCTGAA 
GAGGCTTTAT CCCAAGCAAA AAAGAGTGTT GCCTCCAAAA CCGAAGTTAA AAAAACCCGA 
CGACCAAGAT CTGTGCTGAA TACTCAGCCA GAACAGACCA ATGCAGGGGA GGTGGCCTCC 
TCACTCTCAA GTACTGAAAT TCGGAGACAT AGCCAGAGGC GGCACACAAG TGCGGAAGAG 
GAAGAGCCAC CGCCTGTTAA AATAGCCTGG AAAACTGCGG CAGCAAGGAA ATCCTTACCA 
GCTTCCATTA CGATGCACAA AGGGAGCCTG GATTTGCAGA AGTGTAACAT GTCTCCAGTT 
GTGAAAATTG AACAAGTGTT TGCTCTTCAG AATGCTACAG GGGATGGGAA ATTTATCGAT 
CAATTTGTTT ATTCAACAAA GGGAATTGGT AACAAAACAG AAATAAGTGT CAGGGGGCAA 
GACAGGCTTA TAATTTCTAC ACCAAACCAG AGAAATGAAA AGCCAACGCA GAGTGTATCA 
TCTCCTGAAG CAACATCTGG TTCTACAGGC TCAGTAGAAA AGAAGCAACA GAGAAGATCA 
ATTAGAACTC GTTCTGAATC AGAGAAATCC ACTGAGGTTG TGCCAAAGAA GAAGATCAAA 
AAGGAGCAGG TTGAAACAGT TCCTCAGGCT ACAGTGAAGA CTGGATTACA GAAAGGGTCG 
GCGGACCGGG GAGTGCAGGG CTCTGTCAGA TTCAGTGACA GCTCCGTCTC CGCAGCGATT 
GAGGAAACTG TGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060248.2
CDS	519..2456
Translation	MDFSFSFMQGIMGNTIQQPPQLIDSANIRQEDAFDNNSDIAEDGGQTPYEATLQQGFQYPATTEDLPPLTNGYPSSISVYETQTKYQSYNQYPNGSANGFGAVRNFSPTDYYHSEIPNTRPHEILEKPSPPQPPPPPSVPQTVIPKKTGSPEIKLKITKTIQNGRELFESSLCGDLLNEVQASEHTKSKHESRKEKRKKSNKHDSSRSEERKSHKIPKLEPEEQNRPNERVDTVSEKPREEPVLKEEAPVQPILSSVPTTEVSTGVKFQVGDLVWSKVGTYPWWPCMVSSDPQLEVHTKINTRGAREYHVQFFSNQPERAWVHEKRVREYKGHKQYEELLAEATKQASNHSEKQKIRKPRPQRERAQWDIGIAHAEKALKMTREERIEQYTFIYIDKQPEEALSQAKKSVASKTEVKKTRRPRSVLNTQPEQTNAGEVASSLSSTEIRRHSQRRHTSAEEEEPPPVKIAWKTAAARKSLPASITMHKGSLDLQKCNMSPVVKIEQVFALQNATGDGKFIDQFVYSTKGIGNKTEISVRGQDRLIISTPNQRNEKPTQSVSSPEATSGSTGSVEKKQQRRSIRTRSESEKSTEVVPKKKIKKEQVETVPQATVKTGLQKGSADRGVQGSVRFSDSSVSAAIEETVD

Target ORF information:

RefSeq Version	NM_017778.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear receptor binding SET domain protein 3 (NSD3), transcript variant short, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017778.2

ORF Insert Sequence:

ATGGATTTCT CTTTCTCTTT CATGCAAGGG ATCATGGGAA ACACAATTCA GCAACCACCT 
CAACTCATTG ACTCCGCCAA CATCCGTCAG GAGGATGCCT TTGATAACAA CAGTGACATT 
GCTGAAGATG GTGGCCAGAC ACCATATGAA GCTACTTTGC AGCAAGGCTT TCAGTACCCA 
GCTACAACAG AAGATCTTCC TCCACTCACA AATGGGTATC CATCATCAAT CAGTGTGTAT 
GAAACTCAAA CCAAATACCA GTCATATAAT CAGTATCCTA ATGGGTCAGC CAATGGCTTT 
GGTGCAGTTA GAAACTTTAG CCCCACTGAC TATTATCATT CAGAAATTCC AAACACAAGA 
CCACATGAAA TTCTGGAAAA ACCTTCCCCT CCACAGCCAC CACCTCCTCC TTCGGTACCA 
CAAACTGTGA TTCCAAAGAA GACTGGCTCA CCTGAAATTA AACTAAAAAT AACCAAAACT 
ATCCAGAATG GCAGGGAATT GTTTGAGTCT TCCCTTTGTG GAGACCTTTT AAATGAAGTA 
CAGGCAAGTG AGCACACGAA ATCAAAGCAT GAAAGCAGAA AAGAAAAGAG GAAAAAAAGC 
AACAAGCATG ACTCATCAAG ATCTGAAGAG CGCAAGTCAC ACAAAATCCC CAAATTAGAA 
CCAGAGGAAC AAAATAGACC AAATGAGAGG GTTGACACTG TATCAGAAAA ACCAAGGGAA 
GAACCAGTAC TAAAAGAGGA AGCCCCAGTT CAGCCAATAC TATCTTCTGT TCCAACAACG 
GAAGTGTCCA CTGGTGTTAA GTTTCAGGTT GGCGATCTTG TGTGGTCCAA GGTGGGAACC 
TATCCTTGGT GGCCTTGTAT GGTTTCAAGT GATCCCCAGC TTGAGGTTCA TACTAAAATT 
AACACAAGAG GTGCCCGAGA ATATCATGTC CAGTTTTTTA GCAACCAGCC AGAGAGGGCG 
TGGGTTCATG AAAAACGGGT ACGAGAGTAT AAAGGTCATA AACAGTATGA AGAATTACTG 
GCTGAGGCAA CCAAACAAGC CAGCAATCAC TCTGAGAAAC AAAAGATTCG GAAACCCCGA 
CCTCAGAGAG AACGTGCTCA GTGGGATATT GGCATTGCCC ATGCAGAGAA AGCATTGAAA 
ATGACTCGAG AAGAAAGAAT AGAACAGTAT ACTTTTATTT ACATTGATAA ACAGCCTGAA 
GAGGCTTTAT CCCAAGCAAA AAAGAGTGTT GCCTCCAAAA CCGAAGTTAA AAAAACCCGA 
CGACCAAGAT CTGTGCTGAA TACTCAGCCA GAACAGACCA ATGCAGGGGA GGTGGCCTCC 
TCACTCTCAA GTACTGAAAT TCGGAGACAT AGCCAGAGGC GGCACACAAG TGCGGAAGAG 
GAAGAGCCAC CGCCTGTTAA AATAGCCTGG AAAACTGCGG CAGCAAGGAA ATCCTTACCA 
GCTTCCATTA CGATGCACAA AGGGAGCCTG GATTTGCAGA AGTGTAACAT GTCTCCAGTT 
GTGAAAATTG AACAAGTGTT TGCTCTTCAG AATGCTACAG GGGATGGGAA ATTTATCGAT 
CAATTTGTTT ATTCAACAAA GGGAATTGGT AACAAAACAG AAATAAGTGT CAGGGGGCAA 
GACAGGCTTA TAATTTCTAC ACCAAACCAG AGAAATGAAA AGCCAACGCA GAGTGTATCA 
TCTCCTGAAG CAACATCTGG TTCTACAGGC TCAGTAGAAA AGAAGCAACA GAGAAGATCA 
ATTAGAACTC GTTCTGAATC AGAGAAATCC ACTGAGGTTG TGCCAAAGAA GAAGATCAAA 
AAGGAGCAGG TTGAAACAGT TCCTCAGGCT ACAGTGAAGA CTGGATTACA GAAAGGGTCG 
GCGGACCGGG GAGTGCAGGG CTCTGTCAGA TTCAGTGACA GCTCCGTCTC CGCAGCGATT 
GAGGAAACTG TGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSD3 ( NM_023034.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_075447.1 Homo sapiens nuclear receptor binding SET domain protein 3 (NSD3), transcript variant long, mRNA.

Price & Availability↑

CloneID	OHu19193
Clone ID Related Accession (Same CDS sequence)	NM_023034.1 , NM_023034.2
Accession Version	NM_023034.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-11
Organism	Homo sapiens(human)
Product	histone-lysine N-methyltransferase NSD3 isoform long
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF332469.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (long) contains an alternative 3' end sequence. The longer protein encoded has a different carboxy terminus than the protein encoded by variant short. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF332469.1, AJ295990.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGATTTCT CTTTCTCTTT CATGCAAGGG ATCATGGGAA ACACAATTCA GCAACCACCT 
CAACTCATTG ACTCCGCCAA CATCCGTCAG GAGGATGCCT TTGATAACAA CAGTGACATT 
GCTGAAGATG GTGGCCAGAC ACCATATGAA GCTACTTTGC AGCAAGGCTT TCAGTACCCA 
GCTACAACAG AAGATCTTCC TCCACTCACA AATGGGTATC CATCATCAAT CAGTGTGTAT 
GAAACTCAAA CCAAATACCA GTCATATAAT CAGTATCCTA ATGGGTCAGC CAATGGCTTT 
GGTGCAGTTA GAAACTTTAG CCCCACTGAC TATTATCATT CAGAAATTCC AAACACAAGA 
CCACATGAAA TTCTGGAAAA ACCTTCCCCT CCACAGCCAC CACCTCCTCC TTCGGTACCA 
CAAACTGTGA TTCCAAAGAA GACTGGCTCA CCTGAAATTA AACTAAAAAT AACCAAAACT 
ATCCAGAATG GCAGGGAATT GTTTGAGTCT TCCCTTTGTG GAGACCTTTT AAATGAAGTA 
CAGGCAAGTG AGCACACGAA ATCAAAGCAT GAAAGCAGAA AAGAAAAGAG GAAAAAAAGC 
AACAAGCATG ACTCATCAAG ATCTGAAGAG CGCAAGTCAC ACAAAATCCC CAAATTAGAA 
CCAGAGGAAC AAAATAGACC AAATGAGAGG GTTGACACTG TATCAGAAAA ACCAAGGGAA 
GAACCAGTAC TAAAAGAGGA AGCCCCAGTT CAGCCAATAC TATCTTCTGT TCCAACAACG 
GAAGTGTCCA CTGGTGTTAA GTTTCAGGTT GGCGATCTTG TGTGGTCCAA GGTGGGAACC 
TATCCTTGGT GGCCTTGTAT GGTTTCAAGT GATCCCCAGC TTGAGGTTCA TACTAAAATT 
AACACAAGAG GTGCCCGAGA ATATCATGTC CAGTTTTTTA GCAACCAGCC AGAGAGGGCG 
TGGGTTCATG AAAAACGGGT ACGAGAGTAT AAAGGTCATA AACAGTATGA AGAATTACTG 
GCTGAGGCAA CCAAACAAGC CAGCAATCAC TCTGAGAAAC AAAAGATTCG GAAACCCCGA 
CCTCAGAGAG AACGTGCTCA GTGGGATATT GGCATTGCCC ATGCAGAGAA AGCATTGAAA 
ATGACTCGAG AAGAAAGAAT AGAACAGTAT ACTTTTATTT ACATTGATAA ACAGCCTGAA 
GAGGCTTTAT CCCAAGCAAA AAAGAGTGTT GCCTCCAAAA CCGAAGTTAA AAAAACCCGA 
CGACCAAGAT CTGTGCTGAA TACTCAGCCA GAACAGACCA ATGCAGGGGA GGTGGCCTCC 
TCACTCTCAA GTACTGAAAT TCGGAGACAT AGCCAGAGGC GGCACACAAG TGCGGAAGAG 
GAAGAGCCAC CGCCTGTTAA AATAGCCTGG AAAACTGCGG CAGCAAGGAA ATCCTTACCA 
GCTTCCATTA CGATGCACAA AGGGAGCCTG GATTTGCAGA AGTGTAACAT GTCTCCAGTT 
GTGAAAATTG AACAAGTGTT TGCTCTTCAG AATGCTACAG GGGATGGGAA ATTTATCGAT 
CAATTTGTTT ATTCAACAAA GGGAATTGGT AACAAAACAG AAATAAGTGT CAGGGGGCAA 
GACAGGCTTA TAATTTCTAC ACCAAACCAG AGAAATGAAA AGCCAACGCA GAGTGTATCA 
TCTCCTGAAG CAACATCTGG TTCTACAGGC TCAGTAGAAA AGAAGCAACA GAGAAGATCA 
ATTAGAACTC GTTCTGAATC AGAGAAATCC ACTGAGGTTG TGCCAAAGAA GAAGATCAAA 
AAGGAGCAGG TTGAAACAGT TCCTCAGGCT ACAGTGAAGA CTGGATTACA GAAAGGTGCC 
AGCGAGATTT CAGATTCCTG TAAACCTCTA AAGAAAAGGA GTCGCGCCTC AACTGATGTA 
GAAATGACTA GTTCAGCATA CAGAGACACA TCTGACTCCG ATTCTAGAGG ACTGAGTGAC 
CTGCAGGTAG GCTTTGGAAA GCAAGTAGAT AGCCCTTCAG CTACTGCAGA TGCAGACGTT 
TCTGATGTGC AGTCCATGGA TTCAAGTTTG TCGAGAAGAG GCACTGGAAT GAGTAAGAAG 
GACACTGTAT GTCAGATTTG TGAAAGCTCT GGTGACTCTC TGATTCCTTG TGAGGGAGAG 
TGCTGCAAAC ACTTTCACCT GGAGTGCCTG GGATTGGCAT CACTTCCTGA TAGCAAGTTC 
ATCTGCATGG AATGTAAAAC TGGGCAGCAC CCATGTTTTT CGTGTAAAGT GTCTGGTAAA 
GATGTGAAGC GTTGTTCTGT TGGTGCTTGT GGGAAATTTT ATCATGAAGC CTGTGTCCGC 
AAATTCCCCA CTGCCATCTT TGAATCAAAA GGATTCCGCT GTCCTCAGCA CTGCTGCTCT 
GCCTGCTCTA TGGAGAAAGA TATCCACAAA GCAAGTAAAG GCCGCATGAT GAGATGTTTA 
AGATGTCCAG TTGCCTATCA CTCTGGAGAT GCTTGCATTG CGGCCGGAAG CATGTTAGTA 
TCCTCCTACA TTCTCATCTG TAGTAATCAT TCCAAACGGA GCAGTAATTC TTCTGCTGTA 
AATGTAGGCT TTTGTTTCGT TTGTGCCAGA GGGCTGATAG TTCAGGACCA TTCAGACCCC 
ATGTTCAGTT CATATGCCTA TAAGTCCCAC TACCTACTGA ATGAATCAAA TCGTGCTGAG 
TTGATGAAAT TACCTATGAT TCCTTCTTCG TCAGCTTCCA AAAAGAAATG TGAGAAAGGT 
GGAAGATTGC TCTGCTGTGA ATCGTGCCCA GCTTCCTTCC ACCCGGAATG CCTAAGCATA 
GAAATGCCAG AAGGCTGCTG GAATTGTAAT GACTGTAAAG CTGGCAAGAA ACTACATTAC 
AAGCAGATTG TTTGGGTCAA ATTGGGAAAT TACAGATGGT GGCCAGCAGA GATCTGCAAC 
CCCAGGTCTG TGCCACTGAA CATCCAGGGC CTTAAACATG ACTTGGGGGA CTTCCCTGTA 
TTCTTCTTTG GTTCTCATGA CTACTACTGG GTACACCAGG GCAGAGTGTT CCCTTATGTT 
GAAGGAGACA AAAGCTTTGC TGAAGGGCAG ACTAGTATTA ACAAGACCTT CAAAAAGGCA 
CTGGAAGAAG CTGCAAAACG TTTCCAGGAA TTGAAAGCAC AAAGAGAAAG TAAAGAAGCC 
CTAGAGATTG AAAAAAACTC AAGAAAACCC CCTCCCTACA AACACATCAA AGCTAACAAA 
GTAATAGGAA AGGTGCAGAT CCAGGTTGCT GACCTGTCAG AGATTCCCCG CTGTAACTGC 
AAGCCAGCTG ATGAAAACCC TTGTGGCTTG GAATCGGAGT GCCTGAACAG AATGTTGCAG 
TATGAATGCC ACCCGCAGGT GTGCCCAGCT GGAGATCGTT GTCAGAACCA GTGCTTTACA 
AAGAGACTAT ACCCTGATGC AGAGATCATC AAAACGGAGC GGAGAGGCTG GGGCCTCAGG 
ACCAAAAGGA GCATTAAGAA GGGTGAATTT GTAAATGAAT ACGTCGGTGA ATTAATTGAT 
GAAGAAGAAT GCAGATTGCG AATCAAGCGA GCCCACGAGA ACAGTGTAAC TAATTTTTAT 
ATGTTAACTG TTACCAAGGA CCGTATAATT GATGCCGGCC CAAAAGGAAA TTATTCTCGC 
TTCATGAACC ACAGTTGTAA TCCCAACTGT GAAACACAAA AGTGGACAGT GAATGGAGAT 
GTTCGAGTGG GACTATTTGC TCTCTGTGAT ATTCCTGCAG GGATGGAGTT AACATTTAAT 
TATAACCTAG ATTGTCTGGG CAACGGCAGA ACGGAGTGCC ACTGTGGAGC AGATAACTGC 
AGTGGTTTTC TAGGAGTGCG GCCAAAGTCG GCATGTGCGT CAACAAATGA AGAGAAGGCA 
AAAAATGCTA AGTTAAAACA GAAGAGACGA AAGATCAAAA CAGAACCAAA GCAGATGCAT 
GAAGATTACT GTTTTCAATG TGGAGATGGT GGAGAGCTGG TCATGTGTGA CAAAAAAGAC 
TGTCCCAAAG CATACCACCT CCTATGCCTT AACCTGACTC AGCCACCATA TGGAAAGTGG 
GAGTGTCCGT GGCATCAGTG CGATGAGTGC AGCAGTGCAG CTGTTTCCTT CTGTGAATTC 
TGTCCACATT CATTTTGTAA AGATCATGAA AAGGGGGCCC TGGTTCCCTC TGCACTGGAA 
GGCCGCCTCT GCTGCTCGGA ACATGACCCC ATGGCTCCTG TGTCACCAGA ATACTGGAGC 
AAGATAAAAT GTAAATGGGA ATCACAAGAT CATGGAGAAG AAGTAAAAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075447.1
CDS	519..4832
Translation	MDFSFSFMQGIMGNTIQQPPQLIDSANIRQEDAFDNNSDIAEDGGQTPYEATLQQGFQYPATTEDLPPLTNGYPSSISVYETQTKYQSYNQYPNGSANGFGAVRNFSPTDYYHSEIPNTRPHEILEKPSPPQPPPPPSVPQTVIPKKTGSPEIKLKITKTIQNGRELFESSLCGDLLNEVQASEHTKSKHESRKEKRKKSNKHDSSRSEERKSHKIPKLEPEEQNRPNERVDTVSEKPREEPVLKEEAPVQPILSSVPTTEVSTGVKFQVGDLVWSKVGTYPWWPCMVSSDPQLEVHTKINTRGAREYHVQFFSNQPERAWVHEKRVREYKGHKQYEELLAEATKQASNHSEKQKIRKPRPQRERAQWDIGIAHAEKALKMTREERIEQYTFIYIDKQPEEALSQAKKSVASKTEVKKTRRPRSVLNTQPEQTNAGEVASSLSSTEIRRHSQRRHTSAEEEEPPPVKIAWKTAAARKSLPASITMHKGSLDLQKCNMSPVVKIEQVFALQNATGDGKFIDQFVYSTKGIGNKTEISVRGQDRLIISTPNQRNEKPTQSVSSPEATSGSTGSVEKKQQRRSIRTRSESEKSTEVVPKKKIKKEQVETVPQATVKTGLQKGASEISDSCKPLKKRSRASTDVEMTSSAYRDTSDSDSRGLSDLQVGFGKQVDSPSATADADVSDVQSMDSSLSRRGTGMSKKDTVCQICESSGDSLIPCEGECCKHFHLECLGLASLPDSKFICMECKTGQHPCFSCKVSGKDVKRCSVGACGKFYHEACVRKFPTAIFESKGFRCPQHCCSACSMEKDIHKASKGRMMRCLRCPVAYHSGDACIAAGSMLVSSYILICSNHSKRSSNSSAVNVGFCFVCARGLIVQDHSDPMFSSYAYKSHYLLNESNRAELMKLPMIPSSSASKKKCEKGGRLLCCESCPASFHPECLSIEMPEGCWNCNDCKAGKKLHYKQIVWVKLGNYRWWPAEICNPRSVPLNIQGLKHDLGDFPVFFFGSHDYYWVHQGRVFPYVEGDKSFAEGQTSINKTFKKALEEAAKRFQELKAQRESKEALEIEKNSRKPPPYKHIKANKVIGKVQIQVADLSEIPRCNCKPADENPCGLESECLNRMLQYECHPQVCPAGDRCQNQCFTKRLYPDAEIIKTERRGWGLRTKRSIKKGEFVNEYVGELIDEEECRLRIKRAHENSVTNFYMLTVTKDRIIDAGPKGNYSRFMNHSCNPNCETQKWTVNGDVRVGLFALCDIPAGMELTFNYNLDCLGNGRTECHCGADNCSGFLGVRPKSACASTNEEKAKNAKLKQKRRKIKTEPKQMHEDYCFQCGDGGELVMCDKKDCPKAYHLLCLNLTQPPYGKWECPWHQCDECSSAAVSFCEFCPHSFCKDHEKGALVPSALEGRLCCSEHDPMAPVSPEYWSKIKCKWESQDHGEEVKE

Target ORF information:

RefSeq Version	NM_023034.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear receptor binding SET domain protein 3 (NSD3), transcript variant long, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023034.1

ORF Insert Sequence:

ATGGATTTCT CTTTCTCTTT CATGCAAGGG ATCATGGGAA ACACAATTCA GCAACCACCT 
CAACTCATTG ACTCCGCCAA CATCCGTCAG GAGGATGCCT TTGATAACAA CAGTGACATT 
GCTGAAGATG GTGGCCAGAC ACCATATGAA GCTACTTTGC AGCAAGGCTT TCAGTACCCA 
GCTACAACAG AAGATCTTCC TCCACTCACA AATGGGTATC CATCATCAAT CAGTGTGTAT 
GAAACTCAAA CCAAATACCA GTCATATAAT CAGTATCCTA ATGGGTCAGC CAATGGCTTT 
GGTGCAGTTA GAAACTTTAG CCCCACTGAC TATTATCATT CAGAAATTCC AAACACAAGA 
CCACATGAAA TTCTGGAAAA ACCTTCCCCT CCACAGCCAC CACCTCCTCC TTCGGTACCA 
CAAACTGTGA TTCCAAAGAA GACTGGCTCA CCTGAAATTA AACTAAAAAT AACCAAAACT 
ATCCAGAATG GCAGGGAATT GTTTGAGTCT TCCCTTTGTG GAGACCTTTT AAATGAAGTA 
CAGGCAAGTG AGCACACGAA ATCAAAGCAT GAAAGCAGAA AAGAAAAGAG GAAAAAAAGC 
AACAAGCATG ACTCATCAAG ATCTGAAGAG CGCAAGTCAC ACAAAATCCC CAAATTAGAA 
CCAGAGGAAC AAAATAGACC AAATGAGAGG GTTGACACTG TATCAGAAAA ACCAAGGGAA 
GAACCAGTAC TAAAAGAGGA AGCCCCAGTT CAGCCAATAC TATCTTCTGT TCCAACAACG 
GAAGTGTCCA CTGGTGTTAA GTTTCAGGTT GGCGATCTTG TGTGGTCCAA GGTGGGAACC 
TATCCTTGGT GGCCTTGTAT GGTTTCAAGT GATCCCCAGC TTGAGGTTCA TACTAAAATT 
AACACAAGAG GTGCCCGAGA ATATCATGTC CAGTTTTTTA GCAACCAGCC AGAGAGGGCG 
TGGGTTCATG AAAAACGGGT ACGAGAGTAT AAAGGTCATA AACAGTATGA AGAATTACTG 
GCTGAGGCAA CCAAACAAGC CAGCAATCAC TCTGAGAAAC AAAAGATTCG GAAACCCCGA 
CCTCAGAGAG AACGTGCTCA GTGGGATATT GGCATTGCCC ATGCAGAGAA AGCATTGAAA 
ATGACTCGAG AAGAAAGAAT AGAACAGTAT ACTTTTATTT ACATTGATAA ACAGCCTGAA 
GAGGCTTTAT CCCAAGCAAA AAAGAGTGTT GCCTCCAAAA CCGAAGTTAA AAAAACCCGA 
CGACCAAGAT CTGTGCTGAA TACTCAGCCA GAACAGACCA ATGCAGGGGA GGTGGCCTCC 
TCACTCTCAA GTACTGAAAT TCGGAGACAT AGCCAGAGGC GGCACACAAG TGCGGAAGAG 
GAAGAGCCAC CGCCTGTTAA AATAGCCTGG AAAACTGCGG CAGCAAGGAA ATCCTTACCA 
GCTTCCATTA CGATGCACAA AGGGAGCCTG GATTTGCAGA AGTGTAACAT GTCTCCAGTT 
GTGAAAATTG AACAAGTGTT TGCTCTTCAG AATGCTACAG GGGATGGGAA ATTTATCGAT 
CAATTTGTTT ATTCAACAAA GGGAATTGGT AACAAAACAG AAATAAGTGT CAGGGGGCAA 
GACAGGCTTA TAATTTCTAC ACCAAACCAG AGAAATGAAA AGCCAACGCA GAGTGTATCA 
TCTCCTGAAG CAACATCTGG TTCTACAGGC TCAGTAGAAA AGAAGCAACA GAGAAGATCA 
ATTAGAACTC GTTCTGAATC AGAGAAATCC ACTGAGGTTG TGCCAAAGAA GAAGATCAAA 
AAGGAGCAGG TTGAAACAGT TCCTCAGGCT ACAGTGAAGA CTGGATTACA GAAAGGTGCC 
AGCGAGATTT CAGATTCCTG TAAACCTCTA AAGAAAAGGA GTCGCGCCTC AACTGATGTA 
GAAATGACTA GTTCAGCATA CAGAGACACA TCTGACTCCG ATTCTAGAGG ACTGAGTGAC 
CTGCAGGTAG GCTTTGGAAA GCAAGTAGAT AGCCCTTCAG CTACTGCAGA TGCAGACGTT 
TCTGATGTGC AGTCCATGGA TTCAAGTTTG TCGAGAAGAG GCACTGGAAT GAGTAAGAAG 
GACACTGTAT GTCAGATTTG TGAAAGCTCT GGTGACTCTC TGATTCCTTG TGAGGGAGAG 
TGCTGCAAAC ACTTTCACCT GGAGTGCCTG GGATTGGCAT CACTTCCTGA TAGCAAGTTC 
ATCTGCATGG AATGTAAAAC TGGGCAGCAC CCATGTTTTT CGTGTAAAGT GTCTGGTAAA 
GATGTGAAGC GTTGTTCTGT TGGTGCTTGT GGGAAATTTT ATCATGAAGC CTGTGTCCGC 
AAATTCCCCA CTGCCATCTT TGAATCAAAA GGATTCCGCT GTCCTCAGCA CTGCTGCTCT 
GCCTGCTCTA TGGAGAAAGA TATCCACAAA GCAAGTAAAG GCCGCATGAT GAGATGTTTA 
AGATGTCCAG TTGCCTATCA CTCTGGAGAT GCTTGCATTG CGGCCGGAAG CATGTTAGTA 
TCCTCCTACA TTCTCATCTG TAGTAATCAT TCCAAACGGA GCAGTAATTC TTCTGCTGTA 
AATGTAGGCT TTTGTTTCGT TTGTGCCAGA GGGCTGATAG TTCAGGACCA TTCAGACCCC 
ATGTTCAGTT CATATGCCTA TAAGTCCCAC TACCTACTGA ATGAATCAAA TCGTGCTGAG 
TTGATGAAAT TACCTATGAT TCCTTCTTCG TCAGCTTCCA AAAAGAAATG TGAGAAAGGT 
GGAAGATTGC TCTGCTGTGA ATCGTGCCCA GCTTCCTTCC ACCCGGAATG CCTAAGCATA 
GAAATGCCAG AAGGCTGCTG GAATTGTAAT GACTGTAAAG CTGGCAAGAA ACTACATTAC 
AAGCAGATTG TTTGGGTCAA ATTGGGAAAT TACAGATGGT GGCCAGCAGA GATCTGCAAC 
CCCAGGTCTG TGCCACTGAA CATCCAGGGC CTTAAACATG ACTTGGGGGA CTTCCCTGTA 
TTCTTCTTTG GTTCTCATGA CTACTACTGG GTACACCAGG GCAGAGTGTT CCCTTATGTT 
GAAGGAGACA AAAGCTTTGC TGAAGGGCAG ACTAGTATTA ACAAGACCTT CAAAAAGGCA 
CTGGAAGAAG CTGCAAAACG TTTCCAGGAA TTGAAAGCAC AAAGAGAAAG TAAAGAAGCC 
CTAGAGATTG AAAAAAACTC AAGAAAACCC CCTCCCTACA AACACATCAA AGCTAACAAA 
GTAATAGGAA AGGTGCAGAT CCAGGTTGCT GACCTGTCAG AGATTCCCCG CTGTAACTGC 
AAGCCAGCTG ATGAAAACCC TTGTGGCTTG GAATCGGAGT GCCTGAACAG AATGTTGCAG 
TATGAATGCC ACCCGCAGGT GTGCCCAGCT GGAGATCGTT GTCAGAACCA GTGCTTTACA 
AAGAGACTAT ACCCTGATGC AGAGATCATC AAAACGGAGC GGAGAGGCTG GGGCCTCAGG 
ACCAAAAGGA GCATTAAGAA GGGTGAATTT GTAAATGAAT ACGTCGGTGA ATTAATTGAT 
GAAGAAGAAT GCAGATTGCG AATCAAGCGA GCCCACGAGA ACAGTGTAAC TAATTTTTAT 
ATGTTAACTG TTACCAAGGA CCGTATAATT GATGCCGGCC CAAAAGGAAA TTATTCTCGC 
TTCATGAACC ACAGTTGTAA TCCCAACTGT GAAACACAAA AGTGGACAGT GAATGGAGAT 
GTTCGAGTGG GACTATTTGC TCTCTGTGAT ATTCCTGCAG GGATGGAGTT AACATTTAAT 
TATAACCTAG ATTGTCTGGG CAACGGCAGA ACGGAGTGCC ACTGTGGAGC AGATAACTGC 
AGTGGTTTTC TAGGAGTGCG GCCAAAGTCG GCATGTGCGT CAACAAATGA AGAGAAGGCA 
AAAAATGCTA AGTTAAAACA GAAGAGACGA AAGATCAAAA CAGAACCAAA GCAGATGCAT 
GAAGATTACT GTTTTCAATG TGGAGATGGT GGAGAGCTGG TCATGTGTGA CAAAAAAGAC 
TGTCCCAAAG CATACCACCT CCTATGCCTT AACCTGACTC AGCCACCATA TGGAAAGTGG 
GAGTGTCCGT GGCATCAGTG CGATGAGTGC AGCAGTGCAG CTGTTTCCTT CTGTGAATTC 
TGTCCACATT CATTTTGTAA AGATCATGAA AAGGGGGCCC TGGTTCCCTC TGCACTGGAA 
GGCCGCCTCT GCTGCTCGGA ACATGACCCC ATGGCTCCTG TGTCACCAGA ATACTGGAGC 
AAGATAAAAT GTAAATGGGA ATCACAAGAT CATGGAGAAG AAGTAAAAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSD3 ( NM_023034.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_075447.1 Homo sapiens nuclear receptor binding SET domain protein 3 (NSD3), transcript variant long, mRNA.

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CloneID	OHu19193
Clone ID Related Accession (Same CDS sequence)	NM_023034.1 , NM_023034.2
Accession Version	NM_023034.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	histone-lysine N-methyltransferase NSD3 isoform long
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF332469.1 and AC087362.13. On Nov 23, 2018 this sequence version replaced NM_023034.1. Transcript Variant: This variant (long) contains an alternative 3' end sequence. The longer protein encoded has a different carboxy terminus than the protein encoded by variant short. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.23424.1, AF332469.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000317025.13/ ENSP00000313983.7 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGATTTCT CTTTCTCTTT CATGCAAGGG ATCATGGGAA ACACAATTCA GCAACCACCT 
CAACTCATTG ACTCCGCCAA CATCCGTCAG GAGGATGCCT TTGATAACAA CAGTGACATT 
GCTGAAGATG GTGGCCAGAC ACCATATGAA GCTACTTTGC AGCAAGGCTT TCAGTACCCA 
GCTACAACAG AAGATCTTCC TCCACTCACA AATGGGTATC CATCATCAAT CAGTGTGTAT 
GAAACTCAAA CCAAATACCA GTCATATAAT CAGTATCCTA ATGGGTCAGC CAATGGCTTT 
GGTGCAGTTA GAAACTTTAG CCCCACTGAC TATTATCATT CAGAAATTCC AAACACAAGA 
CCACATGAAA TTCTGGAAAA ACCTTCCCCT CCACAGCCAC CACCTCCTCC TTCGGTACCA 
CAAACTGTGA TTCCAAAGAA GACTGGCTCA CCTGAAATTA AACTAAAAAT AACCAAAACT 
ATCCAGAATG GCAGGGAATT GTTTGAGTCT TCCCTTTGTG GAGACCTTTT AAATGAAGTA 
CAGGCAAGTG AGCACACGAA ATCAAAGCAT GAAAGCAGAA AAGAAAAGAG GAAAAAAAGC 
AACAAGCATG ACTCATCAAG ATCTGAAGAG CGCAAGTCAC ACAAAATCCC CAAATTAGAA 
CCAGAGGAAC AAAATAGACC AAATGAGAGG GTTGACACTG TATCAGAAAA ACCAAGGGAA 
GAACCAGTAC TAAAAGAGGA AGCCCCAGTT CAGCCAATAC TATCTTCTGT TCCAACAACG 
GAAGTGTCCA CTGGTGTTAA GTTTCAGGTT GGCGATCTTG TGTGGTCCAA GGTGGGAACC 
TATCCTTGGT GGCCTTGTAT GGTTTCAAGT GATCCCCAGC TTGAGGTTCA TACTAAAATT 
AACACAAGAG GTGCCCGAGA ATATCATGTC CAGTTTTTTA GCAACCAGCC AGAGAGGGCG 
TGGGTTCATG AAAAACGGGT ACGAGAGTAT AAAGGTCATA AACAGTATGA AGAATTACTG 
GCTGAGGCAA CCAAACAAGC CAGCAATCAC TCTGAGAAAC AAAAGATTCG GAAACCCCGA 
CCTCAGAGAG AACGTGCTCA GTGGGATATT GGCATTGCCC ATGCAGAGAA AGCATTGAAA 
ATGACTCGAG AAGAAAGAAT AGAACAGTAT ACTTTTATTT ACATTGATAA ACAGCCTGAA 
GAGGCTTTAT CCCAAGCAAA AAAGAGTGTT GCCTCCAAAA CCGAAGTTAA AAAAACCCGA 
CGACCAAGAT CTGTGCTGAA TACTCAGCCA GAACAGACCA ATGCAGGGGA GGTGGCCTCC 
TCACTCTCAA GTACTGAAAT TCGGAGACAT AGCCAGAGGC GGCACACAAG TGCGGAAGAG 
GAAGAGCCAC CGCCTGTTAA AATAGCCTGG AAAACTGCGG CAGCAAGGAA ATCCTTACCA 
GCTTCCATTA CGATGCACAA AGGGAGCCTG GATTTGCAGA AGTGTAACAT GTCTCCAGTT 
GTGAAAATTG AACAAGTGTT TGCTCTTCAG AATGCTACAG GGGATGGGAA ATTTATCGAT 
CAATTTGTTT ATTCAACAAA GGGAATTGGT AACAAAACAG AAATAAGTGT CAGGGGGCAA 
GACAGGCTTA TAATTTCTAC ACCAAACCAG AGAAATGAAA AGCCAACGCA GAGTGTATCA 
TCTCCTGAAG CAACATCTGG TTCTACAGGC TCAGTAGAAA AGAAGCAACA GAGAAGATCA 
ATTAGAACTC GTTCTGAATC AGAGAAATCC ACTGAGGTTG TGCCAAAGAA GAAGATCAAA 
AAGGAGCAGG TTGAAACAGT TCCTCAGGCT ACAGTGAAGA CTGGATTACA GAAAGGTGCC 
AGCGAGATTT CAGATTCCTG TAAACCTCTA AAGAAAAGGA GTCGCGCCTC AACTGATGTA 
GAAATGACTA GTTCAGCATA CAGAGACACA TCTGACTCCG ATTCTAGAGG ACTGAGTGAC 
CTGCAGGTAG GCTTTGGAAA GCAAGTAGAT AGCCCTTCAG CTACTGCAGA TGCAGACGTT 
TCTGATGTGC AGTCCATGGA TTCAAGTTTG TCGAGAAGAG GCACTGGAAT GAGTAAGAAG 
GACACTGTAT GTCAGATTTG TGAAAGCTCT GGTGACTCTC TGATTCCTTG TGAGGGAGAG 
TGCTGCAAAC ACTTTCACCT GGAGTGCCTG GGATTGGCAT CACTTCCTGA TAGCAAGTTC 
ATCTGCATGG AATGTAAAAC TGGGCAGCAC CCATGTTTTT CGTGTAAAGT GTCTGGTAAA 
GATGTGAAGC GTTGTTCTGT TGGTGCTTGT GGGAAATTTT ATCATGAAGC CTGTGTCCGC 
AAATTCCCCA CTGCCATCTT TGAATCAAAA GGATTCCGCT GTCCTCAGCA CTGCTGCTCT 
GCCTGCTCTA TGGAGAAAGA TATCCACAAA GCAAGTAAAG GCCGCATGAT GAGATGTTTA 
AGATGTCCAG TTGCCTATCA CTCTGGAGAT GCTTGCATTG CGGCCGGAAG CATGTTAGTA 
TCCTCCTACA TTCTCATCTG TAGTAATCAT TCCAAACGGA GCAGTAATTC TTCTGCTGTA 
AATGTAGGCT TTTGTTTCGT TTGTGCCAGA GGGCTGATAG TTCAGGACCA TTCAGACCCC 
ATGTTCAGTT CATATGCCTA TAAGTCCCAC TACCTACTGA ATGAATCAAA TCGTGCTGAG 
TTGATGAAAT TACCTATGAT TCCTTCTTCG TCAGCTTCCA AAAAGAAATG TGAGAAAGGT 
GGAAGATTGC TCTGCTGTGA ATCGTGCCCA GCTTCCTTCC ACCCGGAATG CCTAAGCATA 
GAAATGCCAG AAGGCTGCTG GAATTGTAAT GACTGTAAAG CTGGCAAGAA ACTACATTAC 
AAGCAGATTG TTTGGGTCAA ATTGGGAAAT TACAGATGGT GGCCAGCAGA GATCTGCAAC 
CCCAGGTCTG TGCCACTGAA CATCCAGGGC CTTAAACATG ACTTGGGGGA CTTCCCTGTA 
TTCTTCTTTG GTTCTCATGA CTACTACTGG GTACACCAGG GCAGAGTGTT CCCTTATGTT 
GAAGGAGACA AAAGCTTTGC TGAAGGGCAG ACTAGTATTA ACAAGACCTT CAAAAAGGCA 
CTGGAAGAAG CTGCAAAACG TTTCCAGGAA TTGAAAGCAC AAAGAGAAAG TAAAGAAGCC 
CTAGAGATTG AAAAAAACTC AAGAAAACCC CCTCCCTACA AACACATCAA AGCTAACAAA 
GTAATAGGAA AGGTGCAGAT CCAGGTTGCT GACCTGTCAG AGATTCCCCG CTGTAACTGC 
AAGCCAGCTG ATGAAAACCC TTGTGGCTTG GAATCGGAGT GCCTGAACAG AATGTTGCAG 
TATGAATGCC ACCCGCAGGT GTGCCCAGCT GGAGATCGTT GTCAGAACCA GTGCTTTACA 
AAGAGACTAT ACCCTGATGC AGAGATCATC AAAACGGAGC GGAGAGGCTG GGGCCTCAGG 
ACCAAAAGGA GCATTAAGAA GGGTGAATTT GTAAATGAAT ACGTCGGTGA ATTAATTGAT 
GAAGAAGAAT GCAGATTGCG AATCAAGCGA GCCCACGAGA ACAGTGTAAC TAATTTTTAT 
ATGTTAACTG TTACCAAGGA CCGTATAATT GATGCCGGCC CAAAAGGAAA TTATTCTCGC 
TTCATGAACC ACAGTTGTAA TCCCAACTGT GAAACACAAA AGTGGACAGT GAATGGAGAT 
GTTCGAGTGG GACTATTTGC TCTCTGTGAT ATTCCTGCAG GGATGGAGTT AACATTTAAT 
TATAACCTAG ATTGTCTGGG CAACGGCAGA ACGGAGTGCC ACTGTGGAGC AGATAACTGC 
AGTGGTTTTC TAGGAGTGCG GCCAAAGTCG GCATGTGCGT CAACAAATGA AGAGAAGGCA 
AAAAATGCTA AGTTAAAACA GAAGAGACGA AAGATCAAAA CAGAACCAAA GCAGATGCAT 
GAAGATTACT GTTTTCAATG TGGAGATGGT GGAGAGCTGG TCATGTGTGA CAAAAAAGAC 
TGTCCCAAAG CATACCACCT CCTATGCCTT AACCTGACTC AGCCACCATA TGGAAAGTGG 
GAGTGTCCGT GGCATCAGTG CGATGAGTGC AGCAGTGCAG CTGTTTCCTT CTGTGAATTC 
TGTCCACATT CATTTTGTAA AGATCATGAA AAGGGGGCCC TGGTTCCCTC TGCACTGGAA 
GGCCGCCTCT GCTGCTCGGA ACATGACCCC ATGGCTCCTG TGTCACCAGA ATACTGGAGC 
AAGATAAAAT GTAAATGGGA ATCACAAGAT CATGGAGAAG AAGTAAAAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075447.1
CDS	518..4831
Translation	MDFSFSFMQGIMGNTIQQPPQLIDSANIRQEDAFDNNSDIAEDGGQTPYEATLQQGFQYPATTEDLPPLTNGYPSSISVYETQTKYQSYNQYPNGSANGFGAVRNFSPTDYYHSEIPNTRPHEILEKPSPPQPPPPPSVPQTVIPKKTGSPEIKLKITKTIQNGRELFESSLCGDLLNEVQASEHTKSKHESRKEKRKKSNKHDSSRSEERKSHKIPKLEPEEQNRPNERVDTVSEKPREEPVLKEEAPVQPILSSVPTTEVSTGVKFQVGDLVWSKVGTYPWWPCMVSSDPQLEVHTKINTRGAREYHVQFFSNQPERAWVHEKRVREYKGHKQYEELLAEATKQASNHSEKQKIRKPRPQRERAQWDIGIAHAEKALKMTREERIEQYTFIYIDKQPEEALSQAKKSVASKTEVKKTRRPRSVLNTQPEQTNAGEVASSLSSTEIRRHSQRRHTSAEEEEPPPVKIAWKTAAARKSLPASITMHKGSLDLQKCNMSPVVKIEQVFALQNATGDGKFIDQFVYSTKGIGNKTEISVRGQDRLIISTPNQRNEKPTQSVSSPEATSGSTGSVEKKQQRRSIRTRSESEKSTEVVPKKKIKKEQVETVPQATVKTGLQKGASEISDSCKPLKKRSRASTDVEMTSSAYRDTSDSDSRGLSDLQVGFGKQVDSPSATADADVSDVQSMDSSLSRRGTGMSKKDTVCQICESSGDSLIPCEGECCKHFHLECLGLASLPDSKFICMECKTGQHPCFSCKVSGKDVKRCSVGACGKFYHEACVRKFPTAIFESKGFRCPQHCCSACSMEKDIHKASKGRMMRCLRCPVAYHSGDACIAAGSMLVSSYILICSNHSKRSSNSSAVNVGFCFVCARGLIVQDHSDPMFSSYAYKSHYLLNESNRAELMKLPMIPSSSASKKKCEKGGRLLCCESCPASFHPECLSIEMPEGCWNCNDCKAGKKLHYKQIVWVKLGNYRWWPAEICNPRSVPLNIQGLKHDLGDFPVFFFGSHDYYWVHQGRVFPYVEGDKSFAEGQTSINKTFKKALEEAAKRFQELKAQRESKEALEIEKNSRKPPPYKHIKANKVIGKVQIQVADLSEIPRCNCKPADENPCGLESECLNRMLQYECHPQVCPAGDRCQNQCFTKRLYPDAEIIKTERRGWGLRTKRSIKKGEFVNEYVGELIDEEECRLRIKRAHENSVTNFYMLTVTKDRIIDAGPKGNYSRFMNHSCNPNCETQKWTVNGDVRVGLFALCDIPAGMELTFNYNLDCLGNGRTECHCGADNCSGFLGVRPKSACASTNEEKAKNAKLKQKRRKIKTEPKQMHEDYCFQCGDGGELVMCDKKDCPKAYHLLCLNLTQPPYGKWECPWHQCDECSSAAVSFCEFCPHSFCKDHEKGALVPSALEGRLCCSEHDPMAPVSPEYWSKIKCKWESQDHGEEVKE

Target ORF information:

RefSeq Version	NM_023034.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear receptor binding SET domain protein 3 (NSD3), transcript variant long, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023034.2

ORF Insert Sequence:

ATGGATTTCT CTTTCTCTTT CATGCAAGGG ATCATGGGAA ACACAATTCA GCAACCACCT 
CAACTCATTG ACTCCGCCAA CATCCGTCAG GAGGATGCCT TTGATAACAA CAGTGACATT 
GCTGAAGATG GTGGCCAGAC ACCATATGAA GCTACTTTGC AGCAAGGCTT TCAGTACCCA 
GCTACAACAG AAGATCTTCC TCCACTCACA AATGGGTATC CATCATCAAT CAGTGTGTAT 
GAAACTCAAA CCAAATACCA GTCATATAAT CAGTATCCTA ATGGGTCAGC CAATGGCTTT 
GGTGCAGTTA GAAACTTTAG CCCCACTGAC TATTATCATT CAGAAATTCC AAACACAAGA 
CCACATGAAA TTCTGGAAAA ACCTTCCCCT CCACAGCCAC CACCTCCTCC TTCGGTACCA 
CAAACTGTGA TTCCAAAGAA GACTGGCTCA CCTGAAATTA AACTAAAAAT AACCAAAACT 
ATCCAGAATG GCAGGGAATT GTTTGAGTCT TCCCTTTGTG GAGACCTTTT AAATGAAGTA 
CAGGCAAGTG AGCACACGAA ATCAAAGCAT GAAAGCAGAA AAGAAAAGAG GAAAAAAAGC 
AACAAGCATG ACTCATCAAG ATCTGAAGAG CGCAAGTCAC ACAAAATCCC CAAATTAGAA 
CCAGAGGAAC AAAATAGACC AAATGAGAGG GTTGACACTG TATCAGAAAA ACCAAGGGAA 
GAACCAGTAC TAAAAGAGGA AGCCCCAGTT CAGCCAATAC TATCTTCTGT TCCAACAACG 
GAAGTGTCCA CTGGTGTTAA GTTTCAGGTT GGCGATCTTG TGTGGTCCAA GGTGGGAACC 
TATCCTTGGT GGCCTTGTAT GGTTTCAAGT GATCCCCAGC TTGAGGTTCA TACTAAAATT 
AACACAAGAG GTGCCCGAGA ATATCATGTC CAGTTTTTTA GCAACCAGCC AGAGAGGGCG 
TGGGTTCATG AAAAACGGGT ACGAGAGTAT AAAGGTCATA AACAGTATGA AGAATTACTG 
GCTGAGGCAA CCAAACAAGC CAGCAATCAC TCTGAGAAAC AAAAGATTCG GAAACCCCGA 
CCTCAGAGAG AACGTGCTCA GTGGGATATT GGCATTGCCC ATGCAGAGAA AGCATTGAAA 
ATGACTCGAG AAGAAAGAAT AGAACAGTAT ACTTTTATTT ACATTGATAA ACAGCCTGAA 
GAGGCTTTAT CCCAAGCAAA AAAGAGTGTT GCCTCCAAAA CCGAAGTTAA AAAAACCCGA 
CGACCAAGAT CTGTGCTGAA TACTCAGCCA GAACAGACCA ATGCAGGGGA GGTGGCCTCC 
TCACTCTCAA GTACTGAAAT TCGGAGACAT AGCCAGAGGC GGCACACAAG TGCGGAAGAG 
GAAGAGCCAC CGCCTGTTAA AATAGCCTGG AAAACTGCGG CAGCAAGGAA ATCCTTACCA 
GCTTCCATTA CGATGCACAA AGGGAGCCTG GATTTGCAGA AGTGTAACAT GTCTCCAGTT 
GTGAAAATTG AACAAGTGTT TGCTCTTCAG AATGCTACAG GGGATGGGAA ATTTATCGAT 
CAATTTGTTT ATTCAACAAA GGGAATTGGT AACAAAACAG AAATAAGTGT CAGGGGGCAA 
GACAGGCTTA TAATTTCTAC ACCAAACCAG AGAAATGAAA AGCCAACGCA GAGTGTATCA 
TCTCCTGAAG CAACATCTGG TTCTACAGGC TCAGTAGAAA AGAAGCAACA GAGAAGATCA 
ATTAGAACTC GTTCTGAATC AGAGAAATCC ACTGAGGTTG TGCCAAAGAA GAAGATCAAA 
AAGGAGCAGG TTGAAACAGT TCCTCAGGCT ACAGTGAAGA CTGGATTACA GAAAGGTGCC 
AGCGAGATTT CAGATTCCTG TAAACCTCTA AAGAAAAGGA GTCGCGCCTC AACTGATGTA 
GAAATGACTA GTTCAGCATA CAGAGACACA TCTGACTCCG ATTCTAGAGG ACTGAGTGAC 
CTGCAGGTAG GCTTTGGAAA GCAAGTAGAT AGCCCTTCAG CTACTGCAGA TGCAGACGTT 
TCTGATGTGC AGTCCATGGA TTCAAGTTTG TCGAGAAGAG GCACTGGAAT GAGTAAGAAG 
GACACTGTAT GTCAGATTTG TGAAAGCTCT GGTGACTCTC TGATTCCTTG TGAGGGAGAG 
TGCTGCAAAC ACTTTCACCT GGAGTGCCTG GGATTGGCAT CACTTCCTGA TAGCAAGTTC 
ATCTGCATGG AATGTAAAAC TGGGCAGCAC CCATGTTTTT CGTGTAAAGT GTCTGGTAAA 
GATGTGAAGC GTTGTTCTGT TGGTGCTTGT GGGAAATTTT ATCATGAAGC CTGTGTCCGC 
AAATTCCCCA CTGCCATCTT TGAATCAAAA GGATTCCGCT GTCCTCAGCA CTGCTGCTCT 
GCCTGCTCTA TGGAGAAAGA TATCCACAAA GCAAGTAAAG GCCGCATGAT GAGATGTTTA 
AGATGTCCAG TTGCCTATCA CTCTGGAGAT GCTTGCATTG CGGCCGGAAG CATGTTAGTA 
TCCTCCTACA TTCTCATCTG TAGTAATCAT TCCAAACGGA GCAGTAATTC TTCTGCTGTA 
AATGTAGGCT TTTGTTTCGT TTGTGCCAGA GGGCTGATAG TTCAGGACCA TTCAGACCCC 
ATGTTCAGTT CATATGCCTA TAAGTCCCAC TACCTACTGA ATGAATCAAA TCGTGCTGAG 
TTGATGAAAT TACCTATGAT TCCTTCTTCG TCAGCTTCCA AAAAGAAATG TGAGAAAGGT 
GGAAGATTGC TCTGCTGTGA ATCGTGCCCA GCTTCCTTCC ACCCGGAATG CCTAAGCATA 
GAAATGCCAG AAGGCTGCTG GAATTGTAAT GACTGTAAAG CTGGCAAGAA ACTACATTAC 
AAGCAGATTG TTTGGGTCAA ATTGGGAAAT TACAGATGGT GGCCAGCAGA GATCTGCAAC 
CCCAGGTCTG TGCCACTGAA CATCCAGGGC CTTAAACATG ACTTGGGGGA CTTCCCTGTA 
TTCTTCTTTG GTTCTCATGA CTACTACTGG GTACACCAGG GCAGAGTGTT CCCTTATGTT 
GAAGGAGACA AAAGCTTTGC TGAAGGGCAG ACTAGTATTA ACAAGACCTT CAAAAAGGCA 
CTGGAAGAAG CTGCAAAACG TTTCCAGGAA TTGAAAGCAC AAAGAGAAAG TAAAGAAGCC 
CTAGAGATTG AAAAAAACTC AAGAAAACCC CCTCCCTACA AACACATCAA AGCTAACAAA 
GTAATAGGAA AGGTGCAGAT CCAGGTTGCT GACCTGTCAG AGATTCCCCG CTGTAACTGC 
AAGCCAGCTG ATGAAAACCC TTGTGGCTTG GAATCGGAGT GCCTGAACAG AATGTTGCAG 
TATGAATGCC ACCCGCAGGT GTGCCCAGCT GGAGATCGTT GTCAGAACCA GTGCTTTACA 
AAGAGACTAT ACCCTGATGC AGAGATCATC AAAACGGAGC GGAGAGGCTG GGGCCTCAGG 
ACCAAAAGGA GCATTAAGAA GGGTGAATTT GTAAATGAAT ACGTCGGTGA ATTAATTGAT 
GAAGAAGAAT GCAGATTGCG AATCAAGCGA GCCCACGAGA ACAGTGTAAC TAATTTTTAT 
ATGTTAACTG TTACCAAGGA CCGTATAATT GATGCCGGCC CAAAAGGAAA TTATTCTCGC 
TTCATGAACC ACAGTTGTAA TCCCAACTGT GAAACACAAA AGTGGACAGT GAATGGAGAT 
GTTCGAGTGG GACTATTTGC TCTCTGTGAT ATTCCTGCAG GGATGGAGTT AACATTTAAT 
TATAACCTAG ATTGTCTGGG CAACGGCAGA ACGGAGTGCC ACTGTGGAGC AGATAACTGC 
AGTGGTTTTC TAGGAGTGCG GCCAAAGTCG GCATGTGCGT CAACAAATGA AGAGAAGGCA 
AAAAATGCTA AGTTAAAACA GAAGAGACGA AAGATCAAAA CAGAACCAAA GCAGATGCAT 
GAAGATTACT GTTTTCAATG TGGAGATGGT GGAGAGCTGG TCATGTGTGA CAAAAAAGAC 
TGTCCCAAAG CATACCACCT CCTATGCCTT AACCTGACTC AGCCACCATA TGGAAAGTGG 
GAGTGTCCGT GGCATCAGTG CGATGAGTGC AGCAGTGCAG CTGTTTCCTT CTGTGAATTC 
TGTCCACATT CATTTTGTAA AGATCATGAA AAGGGGGCCC TGGTTCCCTC TGCACTGGAA 
GGCCGCCTCT GCTGCTCGGA ACATGACCCC ATGGCTCCTG TGTCACCAGA ATACTGGAGC 
AAGATAAAAT GTAAATGGGA ATCACAAGAT CATGGAGAAG AAGTAAAAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural Mechanism of the Oxygenase JMJD6 Recognition by the Extraterminal (ET) Domain of BRD4.
Scientific reports7(1)16272(2017 Nov)
Konuma T,Yu D,Zhao C,Ju Y,Sharma R,Ren C,Zhang Q,Zhou MM,Zeng L

Silencing of histone methyltransferase NSD3 reduces cell viability in osteosarcoma with induction of apoptosis.
Oncology reports38(5)2796-2802(2017 Nov)
Liu Z,Piao L,Zhuang M,Qiu X,Xu X,Zhang D,Liu M,Ren D

Development of mammary hyperplasia, dysplasia, and invasive ductal carcinoma in transgenic mice expressing the 8p11 amplicon oncogene NSD3.
Breast cancer research and treatment164(2)349-358(2017 Jul)
Turner-Ivey B,Smith EL,Rutkovsky AC,Spruill LS,Mills JN,Ethier SP

WHSC1L1 drives cell cycle progression through transcriptional regulation of CDC6 and CDK2 in squamous cell carcinoma of the head and neck.
Oncotarget7(27)42527-42538(2016 Jul)
Saloura V,Vougiouklakis T,Zewde M,Kiyotani K,Park JH,Gao G,Karrison T,Lingen M,Nakamura Y,Hamamoto R

Amplification of WHSC1L1 regulates expression and estrogen-independent activation of ER? in SUM-44 breast cancer cells and is associated with ER? over-expression in breast cancer.
Molecular oncology10(6)850-65(2016 06)
Irish JC,Mills JN,Turner-Ivey B,Wilson RC,Guest ST,Rutkovsky A,Dombkowski A,Kappler CS,Hardiman G,Ethier SP

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NSD3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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