RETSAT cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RETSAT
Entrez Gene ID	54884
Full Name	retinol saturase
General protein information	Preferred Names retinol saturase Names all-trans-retinol 13,14-reductase PPAR-alpha-regulated and starvation-induced gene protein all-trans-13,14-dihydroretinol saturase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p11.2
Disorder MIM:	617597

mRNA and Protein(s)

mRNA	Protein	Name
NM_017750.3	NP_060220.3	all-trans-retinol 13,14-reductase precursor
NM_017750.4	NP_060220.3	all-trans-retinol 13,14-reductase precursor

Pathways from BioSystems

Homologies

	RETSAT	XP_001083594.1
Canis lupus familiaris (dog)	RETSAT	XP_540198.1
Bos taurus (cattle)	RETSAT	NP_001095749.2
Gallus gallus (chicken)	RETSAT	XP_424187.3
Oryza sativa (rice)	Os03g0841900	NP_001051855.1
Rattus norvegicus (Norway rat)	Retsat	NP_659552.1
Mus musculus (house mouse)	Retsat	NP_080435.3
Arabidopsis thaliana (thale cress)	AT1G57770	NP_176088.2
Xenopus tropicalis (tropical clawed frog)	retsat	NP_001121412.1
Danio rerio (zebrafish)	retsat	NP_001015061.1
Homo sapiens (human)	RETSAT	NP_060220.3
Pan troglodytes (chimpanzee)	RETSAT	XP_525801.2
Danio rerio (zebrafish)	zgc:154169	NP_001071261.1

Gene Ontology
Bibliography

Related articles in PubMed

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Retinol saturase promotes adipogenesis and is downregulated in obesity.
Schupp M, Lefterova MI, Janke J, Leitner K, Cristancho AG, Mullican SE, Qatanani M, Szwergold N, Steger DJ, Curtin JC, Kim RJ, Suh MJ, Suh M, Albert MR, Engeli S, Gudas LJ, Lazar MA
Proceedings of the National Academy of Sciences of the United States of America106(4)1105-10(2009 Jan)

Identification of all-trans-retinol:all-trans-13,14-dihydroretinol saturase.
Moise AR, Kuksa V, Imanishi Y, Palczewski K
The Journal of biological chemistry279(48)50230-42(2004 Nov)

The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.
Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C, Currell B, Deuel B, Dowd P, Eaton D, Foster J, Grimaldi C, Gu Q, Hass PE, Heldens S, Huang A, Kim HS, Klimowski L, Jin Y, Johnson S, Lee J, Lewis L, Liao D, Mark M, Robbie E, Sanchez C, Schoenfeld J, Seshagiri S, Simmons L, Singh J, Smith V, Stinson J, Vagts A, Vandlen R, Watanabe C, Wieand D, Woods K, Xie MH, Yansura D, Yi S, Yu G, Yuan J, Zhang M, Zhang Z, Goddard A, Wood WI, Godowski P, Gray A
Genome research13(10)2265-70(2003 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RetSat plays an important role in the biology of adipocytes, where it favors normal differentiation, yet is reduced in the obese state.
Title: Retinol saturase promotes adipogenesis and is downregulated in obesity.

The following RETSAT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RETSAT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02540	NM_017750.4 Latest version!	Homo sapiens retinol saturase (RETSAT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
View Old Accession Versions >>
OHu02540	NM_017750.3	Homo sapiens retinol saturase (RETSAT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RETSAT ( NM_017750.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060220.3 Homo sapiens retinol saturase (RETSAT), mRNA.

Price & Availability↑

CloneID	OHu02540
Clone ID Related Accession (Same CDS sequence)	NM_017750.3 , NM_017750.4
Accession Version	NM_017750.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	all-trans-retinol 13,14-reductase precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA418052.1, AK075261.1, DB001412.1, BC068517.1 and BQ448420.1. On Sep 17, 2008 this sequence version replaced NM_017750.2. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.95248.1, SRR1803616.14472.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGTGGCTTC CGCTGGTGCT GCTCCTGGCT GTGCTGCTGC TGGCCGTCCT CTGCAAAGTT 
TACTTGGGAC TATTCTCTGG CAGCTCCCCG AATCCTTTCT CCGAAGATGT CAAACGGCCC 
CCAGCGCCCC TGGTAACTGA CAAGGAGGCC AGGAAGAAGG TTCTCAAACA AGCTTTTTCA 
GCCAACCAAG TGCCGGAGAA GCTGGATGTG GTGGTAATTG GCAGTGGCTT TGGGGGCCTG 
GCTGCAGCTG CAATTCTAGC TAAAGCTGGC AAGCGAGTCC TGGTGCTGGA ACAACATACC 
AAGGCAGGGG GCTGCTGTCA TACCTTTGGA AAGAATGGCC TTGAATTTGA CACAGGAATC 
CATTACATTG GGCGTATGGA AGAGGGCAGC ATTGGCCGTT TTATCTTGGA CCAGATCACT 
GAAGGGCAGC TGGACTGGGC TCCCCTGTCC TCTCCTTTTG ACATCATGGT ACTGGAAGGG 
CCCAATGGCC GAAAGGAGTA CCCCATGTAC AGTGGAGAGA AAGCCTACAT TCAGGGCCTC 
AAGGAGAAGT TTCCACAGGA GGAAGCTATC ATTGACAAGT ATATAAAGCT GGTTAAGGTG 
GTATCCAGTG GAGCCCCTCA TGCCATCCTG TTGAAATTCC TCCCATTGCC CGTGGTTCAG 
CTCCTCGACA GGTGTGGGCT GCTGACTCGT TTCTCTCCAT TCCTTCAAGC ATCCACCCAG 
AGCCTGGCTG AGGTCCTGCA GCAGCTGGGG GCCTCCTCTG AGCTCCAGGC AGTACTCAGC 
TACATCTTCC CCACTTACGG TGTCACCCCC AACCACAGTG CCTTTTCCAT GCACGCCCTG 
CTGGTCAACC ACTACATGAA AGGAGGCTTT TATCCCCGAG GGGGTTCCAG TGAAATTGCC 
TTCCACACCA TCCCTGTGAT TCAGCGGGCT GGGGGCGCTG TCCTCACAAA GGCCACTGTG 
CAGAGTGTGT TGCTGGACTC AGCTGGGAAA GCCTGTGGTG TCAGTGTGAA GAAGGGGCAT 
GAGCTGGTGA ACATCTATTG CCCCATCGTG GTCTCCAACG CAGGACTGTT CAACACCTAT 
GAACACCTAC TGCCGGGGAA CGCCCGCTGC CTGCCAGGTG TGAAGCAGCA ACTGGGGACG 
GTGCGGCCCG GCTTAGGCAT GACCTCTGTT TTCATCTGCC TGCGAGGCAC CAAGGAAGAC 
CTGCATCTGC CGTCCACCAA CTACTATGTT TACTATGACA CGGACATGGA CCAGGCGATG 
GAGCGCTACG TCTCCATGCC CAGGGAAGAG GCTGCGGAAC ACATCCCTCT TCTCTTCTTC 
GCTTTCCCAT CAGCCAAAGA TCCGACCTGG GAGGACCGAT TCCCAGGCCG GTCCACCATG 
ATCATGCTCA TACCCACTGC CTACGAGTGG TTTGAGGAGT GGCAGGCGGA GCTGAAGGGA 
AAGCGGGGCA GTGACTATGA GACCTTCAAA AACTCCTTTG TGGAAGCCTC TATGTCAGTG 
GTCCTGAAAC TGTTCCCACA GCTGGAGGGG AAGGTGGAGA GTGTGACTGC AGGATCCCCA 
CTCACCAACC AGTTCTATCT GGCTGCTCCC CGAGGTGCCT GCTACGGGGC TGACCATGAC 
CTGGGCCGCC TGCACCCTTG TGTGATGGCC TCCTTGAGGG CCCAGAGCCC CATCCCCAAC 
CTCTATCTGA CAGGCCAGGA TATCTTCACC TGTGGACTGG TCGGGGCCCT GCAAGGTGCC 
CTGCTGTGCA GCAGCGCCAT CCTGAAGCGG AACTTGTACT CAGACCTTAA GAATCTTGAT 
TCTAGGATCC GGGCACAGAA GAAAAAGAAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060220.3
CDS	192..2024
Translation	MWLPLVLLLAVLLLAVLCKVYLGLFSGSSPNPFSEDVKRPPAPLVTDKEARKKVLKQAFSANQVPEKLDVVVIGSGFGGLAAAAILAKAGKRVLVLEQHTKAGGCCHTFGKNGLEFDTGIHYIGRMEEGSIGRFILDQITEGQLDWAPLSSPFDIMVLEGPNGRKEYPMYSGEKAYIQGLKEKFPQEEAIIDKYIKLVKVVSSGAPHAILLKFLPLPVVQLLDRCGLLTRFSPFLQASTQSLAEVLQQLGASSELQAVLSYIFPTYGVTPNHSAFSMHALLVNHYMKGGFYPRGGSSEIAFHTIPVIQRAGGAVLTKATVQSVLLDSAGKACGVSVKKGHELVNIYCPIVVSNAGLFNTYEHLLPGNARCLPGVKQQLGTVRPGLGMTSVFICLRGTKEDLHLPSTNYYVYYDTDMDQAMERYVSMPREEAAEHIPLLFFAFPSAKDPTWEDRFPGRSTMIMLIPTAYEWFEEWQAELKGKRGSDYETFKNSFVEASMSVVLKLFPQLEGKVESVTAGSPLTNQFYLAAPRGACYGADHDLGRLHPCVMASLRAQSPIPNLYLTGQDIFTCGLVGALQGALLCSSAILKRNLYSDLKNLDSRIRAQKKKN

Target ORF information:

RefSeq Version	NM_017750.3
Organism	Homo sapiens(human)
Definition	Homo sapiens retinol saturase (RETSAT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017750.3

ORF Insert Sequence:

ATGTGGCTTC CGCTGGTGCT GCTCCTGGCT GTGCTGCTGC TGGCCGTCCT CTGCAAAGTT 
TACTTGGGAC TATTCTCTGG CAGCTCCCCG AATCCTTTCT CCGAAGATGT CAAACGGCCC 
CCAGCGCCCC TGGTAACTGA CAAGGAGGCC AGGAAGAAGG TTCTCAAACA AGCTTTTTCA 
GCCAACCAAG TGCCGGAGAA GCTGGATGTG GTGGTAATTG GCAGTGGCTT TGGGGGCCTG 
GCTGCAGCTG CAATTCTAGC TAAAGCTGGC AAGCGAGTCC TGGTGCTGGA ACAACATACC 
AAGGCAGGGG GCTGCTGTCA TACCTTTGGA AAGAATGGCC TTGAATTTGA CACAGGAATC 
CATTACATTG GGCGTATGGA AGAGGGCAGC ATTGGCCGTT TTATCTTGGA CCAGATCACT 
GAAGGGCAGC TGGACTGGGC TCCCCTGTCC TCTCCTTTTG ACATCATGGT ACTGGAAGGG 
CCCAATGGCC GAAAGGAGTA CCCCATGTAC AGTGGAGAGA AAGCCTACAT TCAGGGCCTC 
AAGGAGAAGT TTCCACAGGA GGAAGCTATC ATTGACAAGT ATATAAAGCT GGTTAAGGTG 
GTATCCAGTG GAGCCCCTCA TGCCATCCTG TTGAAATTCC TCCCATTGCC CGTGGTTCAG 
CTCCTCGACA GGTGTGGGCT GCTGACTCGT TTCTCTCCAT TCCTTCAAGC ATCCACCCAG 
AGCCTGGCTG AGGTCCTGCA GCAGCTGGGG GCCTCCTCTG AGCTCCAGGC AGTACTCAGC 
TACATCTTCC CCACTTACGG TGTCACCCCC AACCACAGTG CCTTTTCCAT GCACGCCCTG 
CTGGTCAACC ACTACATGAA AGGAGGCTTT TATCCCCGAG GGGGTTCCAG TGAAATTGCC 
TTCCACACCA TCCCTGTGAT TCAGCGGGCT GGGGGCGCTG TCCTCACAAA GGCCACTGTG 
CAGAGTGTGT TGCTGGACTC AGCTGGGAAA GCCTGTGGTG TCAGTGTGAA GAAGGGGCAT 
GAGCTGGTGA ACATCTATTG CCCCATCGTG GTCTCCAACG CAGGACTGTT CAACACCTAT 
GAACACCTAC TGCCGGGGAA CGCCCGCTGC CTGCCAGGTG TGAAGCAGCA ACTGGGGACG 
GTGCGGCCCG GCTTAGGCAT GACCTCTGTT TTCATCTGCC TGCGAGGCAC CAAGGAAGAC 
CTGCATCTGC CGTCCACCAA CTACTATGTT TACTATGACA CGGACATGGA CCAGGCGATG 
GAGCGCTACG TCTCCATGCC CAGGGAAGAG GCTGCGGAAC ACATCCCTCT TCTCTTCTTC 
GCTTTCCCAT CAGCCAAAGA TCCGACCTGG GAGGACCGAT TCCCAGGCCG GTCCACCATG 
ATCATGCTCA TACCCACTGC CTACGAGTGG TTTGAGGAGT GGCAGGCGGA GCTGAAGGGA 
AAGCGGGGCA GTGACTATGA GACCTTCAAA AACTCCTTTG TGGAAGCCTC TATGTCAGTG 
GTCCTGAAAC TGTTCCCACA GCTGGAGGGG AAGGTGGAGA GTGTGACTGC AGGATCCCCA 
CTCACCAACC AGTTCTATCT GGCTGCTCCC CGAGGTGCCT GCTACGGGGC TGACCATGAC 
CTGGGCCGCC TGCACCCTTG TGTGATGGCC TCCTTGAGGG CCCAGAGCCC CATCCCCAAC 
CTCTATCTGA CAGGCCAGGA TATCTTCACC TGTGGACTGG TCGGGGCCCT GCAAGGTGCC 
CTGCTGTGCA GCAGCGCCAT CCTGAAGCGG AACTTGTACT CAGACCTTAA GAATCTTGAT 
TCTAGGATCC GGGCACAGAA GAAAAAGAAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RETSAT ( NM_017750.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060220.3 Homo sapiens retinol saturase (RETSAT), mRNA.

Price & Availability↑

CloneID	OHu02540
Clone ID Related Accession (Same CDS sequence)	NM_017750.3 , NM_017750.4
Accession Version	NM_017750.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	all-trans-retinol 13,14-reductase precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK075261.1, DB001412.1, BC068517.1 and BQ448420.1. On Nov 23, 2018 this sequence version replaced NM_017750.3. ##Evidence-Data-START## Transcript exon combination :: BC068517.1, SRR1803617.125033.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000295802.9/ ENSP00000295802.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTGGCTTC CGCTGGTGCT GCTCCTGGCT GTGCTGCTGC TGGCCGTCCT CTGCAAAGTT 
TACTTGGGAC TATTCTCTGG CAGCTCCCCG AATCCTTTCT CCGAAGATGT CAAACGGCCC 
CCAGCGCCCC TGGTAACTGA CAAGGAGGCC AGGAAGAAGG TTCTCAAACA AGCTTTTTCA 
GCCAACCAAG TGCCGGAGAA GCTGGATGTG GTGGTAATTG GCAGTGGCTT TGGGGGCCTG 
GCTGCAGCTG CAATTCTAGC TAAAGCTGGC AAGCGAGTCC TGGTGCTGGA ACAACATACC 
AAGGCAGGGG GCTGCTGTCA TACCTTTGGA AAGAATGGCC TTGAATTTGA CACAGGAATC 
CATTACATTG GGCGTATGGA AGAGGGCAGC ATTGGCCGTT TTATCTTGGA CCAGATCACT 
GAAGGGCAGC TGGACTGGGC TCCCCTGTCC TCTCCTTTTG ACATCATGGT ACTGGAAGGG 
CCCAATGGCC GAAAGGAGTA CCCCATGTAC AGTGGAGAGA AAGCCTACAT TCAGGGCCTC 
AAGGAGAAGT TTCCACAGGA GGAAGCTATC ATTGACAAGT ATATAAAGCT GGTTAAGGTG 
GTATCCAGTG GAGCCCCTCA TGCCATCCTG TTGAAATTCC TCCCATTGCC CGTGGTTCAG 
CTCCTCGACA GGTGTGGGCT GCTGACTCGT TTCTCTCCAT TCCTTCAAGC ATCCACCCAG 
AGCCTGGCTG AGGTCCTGCA GCAGCTGGGG GCCTCCTCTG AGCTCCAGGC AGTACTCAGC 
TACATCTTCC CCACTTACGG TGTCACCCCC AACCACAGTG CCTTTTCCAT GCACGCCCTG 
CTGGTCAACC ACTACATGAA AGGAGGCTTT TATCCCCGAG GGGGTTCCAG TGAAATTGCC 
TTCCACACCA TCCCTGTGAT TCAGCGGGCT GGGGGCGCTG TCCTCACAAA GGCCACTGTG 
CAGAGTGTGT TGCTGGACTC AGCTGGGAAA GCCTGTGGTG TCAGTGTGAA GAAGGGGCAT 
GAGCTGGTGA ACATCTATTG CCCCATCGTG GTCTCCAACG CAGGACTGTT CAACACCTAT 
GAACACCTAC TGCCGGGGAA CGCCCGCTGC CTGCCAGGTG TGAAGCAGCA ACTGGGGACG 
GTGCGGCCCG GCTTAGGCAT GACCTCTGTT TTCATCTGCC TGCGAGGCAC CAAGGAAGAC 
CTGCATCTGC CGTCCACCAA CTACTATGTT TACTATGACA CGGACATGGA CCAGGCGATG 
GAGCGCTACG TCTCCATGCC CAGGGAAGAG GCTGCGGAAC ACATCCCTCT TCTCTTCTTC 
GCTTTCCCAT CAGCCAAAGA TCCGACCTGG GAGGACCGAT TCCCAGGCCG GTCCACCATG 
ATCATGCTCA TACCCACTGC CTACGAGTGG TTTGAGGAGT GGCAGGCGGA GCTGAAGGGA 
AAGCGGGGCA GTGACTATGA GACCTTCAAA AACTCCTTTG TGGAAGCCTC TATGTCAGTG 
GTCCTGAAAC TGTTCCCACA GCTGGAGGGG AAGGTGGAGA GTGTGACTGC AGGATCCCCA 
CTCACCAACC AGTTCTATCT GGCTGCTCCC CGAGGTGCCT GCTACGGGGC TGACCATGAC 
CTGGGCCGCC TGCACCCTTG TGTGATGGCC TCCTTGAGGG CCCAGAGCCC CATCCCCAAC 
CTCTATCTGA CAGGCCAGGA TATCTTCACC TGTGGACTGG TCGGGGCCCT GCAAGGTGCC 
CTGCTGTGCA GCAGCGCCAT CCTGAAGCGG AACTTGTACT CAGACCTTAA GAATCTTGAT 
TCTAGGATCC GGGCACAGAA GAAAAAGAAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060220.3
CDS	22..1854
Translation	MWLPLVLLLAVLLLAVLCKVYLGLFSGSSPNPFSEDVKRPPAPLVTDKEARKKVLKQAFSANQVPEKLDVVVIGSGFGGLAAAAILAKAGKRVLVLEQHTKAGGCCHTFGKNGLEFDTGIHYIGRMEEGSIGRFILDQITEGQLDWAPLSSPFDIMVLEGPNGRKEYPMYSGEKAYIQGLKEKFPQEEAIIDKYIKLVKVVSSGAPHAILLKFLPLPVVQLLDRCGLLTRFSPFLQASTQSLAEVLQQLGASSELQAVLSYIFPTYGVTPNHSAFSMHALLVNHYMKGGFYPRGGSSEIAFHTIPVIQRAGGAVLTKATVQSVLLDSAGKACGVSVKKGHELVNIYCPIVVSNAGLFNTYEHLLPGNARCLPGVKQQLGTVRPGLGMTSVFICLRGTKEDLHLPSTNYYVYYDTDMDQAMERYVSMPREEAAEHIPLLFFAFPSAKDPTWEDRFPGRSTMIMLIPTAYEWFEEWQAELKGKRGSDYETFKNSFVEASMSVVLKLFPQLEGKVESVTAGSPLTNQFYLAAPRGACYGADHDLGRLHPCVMASLRAQSPIPNLYLTGQDIFTCGLVGALQGALLCSSAILKRNLYSDLKNLDSRIRAQKKKN

Target ORF information:

RefSeq Version	NM_017750.4
Organism	Homo sapiens(human)
Definition	Homo sapiens retinol saturase (RETSAT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017750.4

ORF Insert Sequence:

ATGTGGCTTC CGCTGGTGCT GCTCCTGGCT GTGCTGCTGC TGGCCGTCCT CTGCAAAGTT 
TACTTGGGAC TATTCTCTGG CAGCTCCCCG AATCCTTTCT CCGAAGATGT CAAACGGCCC 
CCAGCGCCCC TGGTAACTGA CAAGGAGGCC AGGAAGAAGG TTCTCAAACA AGCTTTTTCA 
GCCAACCAAG TGCCGGAGAA GCTGGATGTG GTGGTAATTG GCAGTGGCTT TGGGGGCCTG 
GCTGCAGCTG CAATTCTAGC TAAAGCTGGC AAGCGAGTCC TGGTGCTGGA ACAACATACC 
AAGGCAGGGG GCTGCTGTCA TACCTTTGGA AAGAATGGCC TTGAATTTGA CACAGGAATC 
CATTACATTG GGCGTATGGA AGAGGGCAGC ATTGGCCGTT TTATCTTGGA CCAGATCACT 
GAAGGGCAGC TGGACTGGGC TCCCCTGTCC TCTCCTTTTG ACATCATGGT ACTGGAAGGG 
CCCAATGGCC GAAAGGAGTA CCCCATGTAC AGTGGAGAGA AAGCCTACAT TCAGGGCCTC 
AAGGAGAAGT TTCCACAGGA GGAAGCTATC ATTGACAAGT ATATAAAGCT GGTTAAGGTG 
GTATCCAGTG GAGCCCCTCA TGCCATCCTG TTGAAATTCC TCCCATTGCC CGTGGTTCAG 
CTCCTCGACA GGTGTGGGCT GCTGACTCGT TTCTCTCCAT TCCTTCAAGC ATCCACCCAG 
AGCCTGGCTG AGGTCCTGCA GCAGCTGGGG GCCTCCTCTG AGCTCCAGGC AGTACTCAGC 
TACATCTTCC CCACTTACGG TGTCACCCCC AACCACAGTG CCTTTTCCAT GCACGCCCTG 
CTGGTCAACC ACTACATGAA AGGAGGCTTT TATCCCCGAG GGGGTTCCAG TGAAATTGCC 
TTCCACACCA TCCCTGTGAT TCAGCGGGCT GGGGGCGCTG TCCTCACAAA GGCCACTGTG 
CAGAGTGTGT TGCTGGACTC AGCTGGGAAA GCCTGTGGTG TCAGTGTGAA GAAGGGGCAT 
GAGCTGGTGA ACATCTATTG CCCCATCGTG GTCTCCAACG CAGGACTGTT CAACACCTAT 
GAACACCTAC TGCCGGGGAA CGCCCGCTGC CTGCCAGGTG TGAAGCAGCA ACTGGGGACG 
GTGCGGCCCG GCTTAGGCAT GACCTCTGTT TTCATCTGCC TGCGAGGCAC CAAGGAAGAC 
CTGCATCTGC CGTCCACCAA CTACTATGTT TACTATGACA CGGACATGGA CCAGGCGATG 
GAGCGCTACG TCTCCATGCC CAGGGAAGAG GCTGCGGAAC ACATCCCTCT TCTCTTCTTC 
GCTTTCCCAT CAGCCAAAGA TCCGACCTGG GAGGACCGAT TCCCAGGCCG GTCCACCATG 
ATCATGCTCA TACCCACTGC CTACGAGTGG TTTGAGGAGT GGCAGGCGGA GCTGAAGGGA 
AAGCGGGGCA GTGACTATGA GACCTTCAAA AACTCCTTTG TGGAAGCCTC TATGTCAGTG 
GTCCTGAAAC TGTTCCCACA GCTGGAGGGG AAGGTGGAGA GTGTGACTGC AGGATCCCCA 
CTCACCAACC AGTTCTATCT GGCTGCTCCC CGAGGTGCCT GCTACGGGGC TGACCATGAC 
CTGGGCCGCC TGCACCCTTG TGTGATGGCC TCCTTGAGGG CCCAGAGCCC CATCCCCAAC 
CTCTATCTGA CAGGCCAGGA TATCTTCACC TGTGGACTGG TCGGGGCCCT GCAAGGTGCC 
CTGCTGTGCA GCAGCGCCAT CCTGAAGCGG AACTTGTACT CAGACCTTAA GAATCTTGAT 
TCTAGGATCC GGGCACAGAA GAAAAAGAAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

Retinol saturase promotes adipogenesis and is downregulated in obesity.
Proceedings of the National Academy of Sciences of the United States of America106(4)1105-10(2009 Jan)
Schupp M,Lefterova MI,Janke J,Leitner K,Cristancho AG,Mullican SE,Qatanani M,Szwergold N,Steger DJ,Curtin JC,Kim RJ,Suh MJ,Suh M,Albert MR,Engeli S,Gudas LJ,Lazar MA

Identification of all-trans-retinol:all-trans-13,14-dihydroretinol saturase.
The Journal of biological chemistry279(48)50230-42(2004 Nov)
Moise AR,Kuksa V,Imanishi Y,Palczewski K

The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.
Genome research13(10)2265-70(2003 Oct)
Clark HF,Gurney AL,Abaya E,Baker K,Baldwin D,Brush J,Chen J,Chow B,Chui C,Crowley C,Currell B,Deuel B,Dowd P,Eaton D,Foster J,Grimaldi C,Gu Q,Hass PE,Heldens S,Huang A,Kim HS,Klimowski L,Jin Y,Johnson S,Lee J,Lewis L,Liao D,Mark M,Robbie E,Sanchez C,Schoenfeld J,Seshagiri S,Simmons L,Singh J,Smith V,Stinson J,Vagts A,Vandlen R,Watanabe C,Wieand D,Woods K,Xie MH,Yansura D,Yi S,Yu G,Yuan J,Zhang M,Zhang Z,Goddard A,Wood WI,Godowski P,Gray A

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RETSAT cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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