CNGB3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CNGB3
Entrez Gene ID	54714
Full Name	cyclic nucleotide gated channel beta 3
Synonyms	ACHM1
General protein information	Preferred Names cyclic nucleotide gated channel beta 3 Names cyclic nucleotide-gated cation channel beta-3 CNG channel beta-3 cone photoreceptor cGMP-gated cation channel beta-subunit cyclic nucleotide-gated cation channel modulatory subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8q21.3
Summary	This gene encodes the beta subunit of a cyclic nucleotide-gated ion channel. The encoded beta subunit appears to play a role in modulation of channel function in cone photoreceptors. This heterotetrameric channel is necessary for sensory transduction, and mutations in this gene have been associated with achromatopsia 3, progressive cone dystrophy, and juvenile macular degeneration, also known as Stargardt Disease. [provided by RefSeq, Feb 2010].
Disorder MIM:	605080
Disorder Html:	Achromatopsia 3, 262300 (3); Macular degeneration, juvenile, 248200 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_019098.4	NP_061971.3	cyclic nucleotide-gated cation channel beta-3
XM_011517138.2	XP_011515440.1	cyclic nucleotide-gated cation channel beta-3 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CNGB3	XP_519846.2
Homo sapiens (human)	CNGB3	NP_061971.3
	CNGB3	XP_001083646.1
Bos taurus (cattle)	CNGB3	XP_002692873.2
Danio rerio (zebrafish)	cngb3	XP_691142.4
Canis lupus familiaris (dog)	CNGB3	NP_001003030.1
Rattus norvegicus (Norway rat)	Cngb3	NP_001258167.1
Danio rerio (zebrafish)	LOC562466	XP_690937.4
Xenopus tropicalis (tropical clawed frog)	cngb3	XP_002939202.2
Mus musculus (house mouse)	Cngb3	NP_038955.1
Gallus gallus (chicken)	CNGB3	XP_425928.4

Gene Ontology
Bibliography

Related articles in PubMed

CNGB3 mutations cause severe rod dysfunction.
Maguire J, McKibbin M, Khan K, Kohl S, Ali M, McKeefry D
Ophthalmic genetics39(1)108-114(2018 Jan-Feb)

Safety and Efficacy of AAV5 Vectors Expressing Human or Canine CNGB3 in CNGB3-Mutant Dogs.
Ye GJ, Kom?romy AM, Zeiss C, Calcedo R, Harman CD, Koehl KL, Stewart GA, Iwabe S, Chiodo VA, Hauswirth WW, Aguirre GD, Chulay JD
Human gene therapy. Clinical development28(4)197-207(2017 Dec)

CNGB3 mutation spectrum including copy number variations in 552 achromatopsia patients.
Mayer AK, Van Cauwenbergh C, Rother C, Baumann B, Reuter P, De Baere E, Wissinger B, Kohl S,
Human mutation38(11)1579-1591(2017 Nov)

REPEATABILITY AND LONGITUDINAL ASSESSMENT OF FOVEAL CONE STRUCTURE IN CNGB3-ASSOCIATED ACHROMATOPSIA.
Langlo CS, Erker LR, Parker M, Patterson EJ, Higgins BP, Summerfelt P, Razeen MM, Collison FT, Fishman GA, Kay CN, Zhang J, Weleber RG, Yang P, Pennesi ME, Lam BL, Chulay JD, Dubra A, Hauswirth WW, Wilson DJ, Carroll J,
Retina (Philadelphia, Pa.)37(10)1956-1966(2017 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The degree of residual foveal cone structure varies greatly among subjects with CNGB3-associated ACHM.
Title: Residual Foveal Cone Structure in CNGB3-Associated Achromatopsia.

Genetic testing revealed a common homozygous mutation in CNGB3 in 5 patients with complete achromatopsia and heterozygous mutations in CNGA3 in 2 patients with incomplete achromatopsia.
Title: Retinal morphology of patients with achromatopsia during early childhood: implications for gene therapy.

Eight different mutations were detected in the CNGB3 gene in achromatopsia, including five novel mutations: two splice site mutations, one nonsense substitution, and two frame-shift mutations.
Title: Five novel CNGB3 gene mutations in Polish patients with achromatopsia.

Genetic testing performed at Carver lab at the University of Iowa confirmed a diagnosis of achromatopsia with identical mutations in the CNGB3 gene.
Title: Spectral domain optical coherence tomography findings in CNGB3-associated achromatopsia and therapeutic implications.

Data found pathogenic DNA variants in the genes RP1, USH2A, CNGB3, NMNAT1, CHM, and ABCA4, responsible for retinitis pigmentosa, Usher syndrome, achromatopsia, Leber congenital amaurosis, choroideremia, or recessive Stargardt/cone-rod dystrophy cases.
Title: Exome sequencing of index patients with retinal dystrophies as a tool for molecular diagnosis.

The following CNGB3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CNGB3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu18723	NM_019098.4 Latest version!	Homo sapiens cyclic nucleotide gated channel subunit beta 3 (CNGB3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu68494	XM_011517138.2 Latest version!	Homo sapiens cyclic nucleotide gated channel subunit beta 3 (CNGB3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CNGB3 ( NM_019098.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_061971.3 Homo sapiens cyclic nucleotide gated channel subunit beta 3 (CNGB3), mRNA.

Price & Availability↑

CloneID	OHu18723
Clone ID Related Accession (Same CDS sequence)	NM_019098.4
Accession Version	NM_019098.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	cyclic nucleotide-gated cation channel beta-3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL713036.1, AF272900.1, AF228520.1 and BX118844.1. This sequence is a reference standard in the RefSeqGene project. On Feb 19, 2010 this sequence version replaced NM_019098.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF272900.1, BC150601.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTTAAAT CGCTGACAAA AGTCAACAAG GTGAAGCCTA TAGGAGAGAA CAATGAGAAT 
GAACAAAGTT CTCGTCGGAA TGAAGAAGGC TCTCACCCAA GTAATCAGTC TCAGCAAACC 
ACAGCACAGG AAGAAAACAA AGGTGAAGAG AAATCTCTCA AAACCAAGTC AACTCCAGTC 
ACGTCTGAAG AGCCACACAC CAACATACAA GACAAACTCT CCAAGAAAAA TTCCTCTGGA 
GATCTGACCA CAAACCCTGA CCCTCAAAAT GCAGCAGAAC CAACTGGAAC AGTGCCAGAG 
CAGAAGGAAA TGGACCCCGG GAAAGAAGGT CCAAACAGCC CACAAAACAA ACCGCCTGCA 
GCTCCTGTTA TAAATGAGTA TGCCGATGCC CAGCTACACA ACCTGGTGAA AAGAATGCGT 
CAAAGAACAG CCCTCTACAA GAAAAAGTTG GTAGAGGGAG ATCTCTCCTC ACCCGAAGCC 
AGCCCACAAA CTGCAAAGCC CACGGCTGTA CCACCAGTAA AAGAAAGCGA TGATAAGCCA 
ACAGAACATT ACTACAGGCT GTTGTGGTTC AAAGTCAAAA AGATGCCTTT AACAGAGTAC 
TTAAAGCGAA TTAAACTTCC AAACAGCATA GATTCATACA CAGATCGACT CTATCTCCTG 
TGGCTCTTGC TTGTCACTCT TGCCTATAAC TGGAACTGCT GTTTTATACC ACTGCGCCTC 
GTCTTCCCAT ATCAAACCGC AGACAACATA CACTACTGGC TTATTGCGGA CATCATATGT 
GATATCATCT ACCTTTATGA TATGCTATTT ATCCAGCCCA GACTCCAGTT TGTAAGAGGA 
GGAGACATAA TAGTGGATTC AAATGAGCTA AGGAAACACT ACAGGACTTC TACAAAATTT 
CAGTTGGATG TCGCATCAAT AATACCATTT GATATTTGCT ACCTCTTCTT TGGGTTTAAT 
CCAATGTTTA GAGCAAATAG GATGTTAAAG TACACTTCAT TTTTTGAATT TAATCATCAC 
CTAGAGTCTA TAATGGACAA AGCATATATC TACAGAGTTA TTCGAACAAC TGGATACTTG 
CTGTTTATTC TGCACATTAA TGCCTGTGTT TATTACTGGG CTTCAAACTA TGAAGGAATT 
GGCACTACTA GATGGGTGTA TGATGGGGAA GGAAACGAGT ATCTGAGATG TTATTATTGG 
GCAGTTCGAA CTTTAATTAC CATTGGTGGC CTTCCAGAAC CACAAACTTT ATTTGAAATT 
GTTTTTCAAC TCTTGAATTT TTTTTCTGGA GTTTTTGTGT TCTCCAGTTT AATTGGTCAG 
ATGAGAGATG TGATTGGAGC AGCTACAGCC AATCAGAACT ACTTCCGCGC CTGCATGGAT 
GACACCATTG CCTACATGAA CAATTACTCC ATTCCTAAAC TTGTGCAAAA GCGAGTTCGG 
ACTTGGTATG AATATACATG GGACTCTCAA AGAATGCTAG ATGAGTCTGA TTTGCTTAAG 
ACCCTACCAA CTACGGTCCA GTTAGCCCTC GCCATTGATG TGAACTTCAG CATCATCAGC 
AAAGTCGACT TGTTCAAGGG TTGTGATACA CAGATGATTT ATGACATGTT GCTAAGATTG 
AAATCCGTTC TCTATTTGCC TGGTGACTTT GTCTGCAAAA AGGGAGAAAT TGGCAAGGAA 
ATGTATATCA TCAAGCATGG AGAAGTCCAA GTTCTTGGAG GCCCTGATGG TACTAAAGTT 
CTGGTTACTC TGAAAGCTGG GTCGGTGTTT GGAGAAATCA GCCTTCTAGC AGCAGGAGGA 
GGAAACCGTC GAACTGCCAA TGTGGTGGCC CACGGGTTTG CCAATCTTTT AACTCTAGAC 
AAAAAGACCC TCCAAGAAAT TCTAGTGCAT TATCCAGATT CTGAAAGGAT CCTCATGAAG 
AAAGCCAGAG TGCTTTTAAA GCAGAAGGCT AAGACCGCAG AAGCAACCCC TCCAAGAAAA 
GATCTTGCCC TCCTCTTCCC ACCGAAAGAA GAGACACCCA AACTGTTTAA AACTCTCCTA 
GGAGGCACAG GAAAAGCAAG TCTTGCAAGA CTACTCAAAT TGAAGCGAGA GCAAGCAGCT 
CAGAAGAAAG AAAATTCTGA AGGAGGAGAG GAAGAAGGAA AAGAAAATGA AGATAAACAA 
AAAGAAAATG AAGATAAACA AAAAGAAAAT GAAGATAAAG GAAAAGAAAA TGAAGATAAA 
GATAAAGGAA GAGAGCCAGA AGAGAAGCCA CTGGACAGAC CTGAATGTAC AGCAAGTCCT 
ATTGCAGTGG AGGAAGAACC CCACTCAGTT AGAAGGACAG TTTTACCCAG AGGGACTTCT 
CGTCAATCAC TCATTATCAG CATGGCTCCT TCTGCTGAGG GCGGAGAAGA GGTTCTTACT 
ATTGAAGTCA AAGAAAAGGC TAAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061971.3
CDS	49..2478
Translation	MFKSLTKVNKVKPIGENNENEQSSRRNEEGSHPSNQSQQTTAQEENKGEEKSLKTKSTPVTSEEPHTNIQDKLSKKNSSGDLTTNPDPQNAAEPTGTVPEQKEMDPGKEGPNSPQNKPPAAPVINEYADAQLHNLVKRMRQRTALYKKKLVEGDLSSPEASPQTAKPTAVPPVKESDDKPTEHYYRLLWFKVKKMPLTEYLKRIKLPNSIDSYTDRLYLLWLLLVTLAYNWNCCFIPLRLVFPYQTADNIHYWLIADIICDIIYLYDMLFIQPRLQFVRGGDIIVDSNELRKHYRTSTKFQLDVASIIPFDICYLFFGFNPMFRANRMLKYTSFFEFNHHLESIMDKAYIYRVIRTTGYLLFILHINACVYYWASNYEGIGTTRWVYDGEGNEYLRCYYWAVRTLITIGGLPEPQTLFEIVFQLLNFFSGVFVFSSLIGQMRDVIGAATANQNYFRACMDDTIAYMNNYSIPKLVQKRVRTWYEYTWDSQRMLDESDLLKTLPTTVQLALAIDVNFSIISKVDLFKGCDTQMIYDMLLRLKSVLYLPGDFVCKKGEIGKEMYIIKHGEVQVLGGPDGTKVLVTLKAGSVFGEISLLAAGGGNRRTANVVAHGFANLLTLDKKTLQEILVHYPDSERILMKKARVLLKQKAKTAEATPPRKDLALLFPPKEETPKLFKTLLGGTGKASLARLLKLKREQAAQKKENSEGGEEEGKENEDKQKENEDKQKENEDKGKENEDKDKGREPEEKPLDRPECTASPIAVEEEPHSVRRTVLPRGTSRQSLIISMAPSAEGGEEVLTIEVKEKAKQ

Target ORF information:

RefSeq Version	NM_019098.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cyclic nucleotide gated channel subunit beta 3 (CNGB3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019098.4

ORF Insert Sequence:

ATGTTTAAAT CGCTGACAAA AGTCAACAAG GTGAAGCCTA TAGGAGAGAA CAATGAGAAT 
GAACAAAGTT CTCGTCGGAA TGAAGAAGGC TCTCACCCAA GTAATCAGTC TCAGCAAACC 
ACAGCACAGG AAGAAAACAA AGGTGAAGAG AAATCTCTCA AAACCAAGTC AACTCCAGTC 
ACGTCTGAAG AGCCACACAC CAACATACAA GACAAACTCT CCAAGAAAAA TTCCTCTGGA 
GATCTGACCA CAAACCCTGA CCCTCAAAAT GCAGCAGAAC CAACTGGAAC AGTGCCAGAG 
CAGAAGGAAA TGGACCCCGG GAAAGAAGGT CCAAACAGCC CACAAAACAA ACCGCCTGCA 
GCTCCTGTTA TAAATGAGTA TGCCGATGCC CAGCTACACA ACCTGGTGAA AAGAATGCGT 
CAAAGAACAG CCCTCTACAA GAAAAAGTTG GTAGAGGGAG ATCTCTCCTC ACCCGAAGCC 
AGCCCACAAA CTGCAAAGCC CACGGCTGTA CCACCAGTAA AAGAAAGCGA TGATAAGCCA 
ACAGAACATT ACTACAGGCT GTTGTGGTTC AAAGTCAAAA AGATGCCTTT AACAGAGTAC 
TTAAAGCGAA TTAAACTTCC AAACAGCATA GATTCATACA CAGATCGACT CTATCTCCTG 
TGGCTCTTGC TTGTCACTCT TGCCTATAAC TGGAACTGCT GTTTTATACC ACTGCGCCTC 
GTCTTCCCAT ATCAAACCGC AGACAACATA CACTACTGGC TTATTGCGGA CATCATATGT 
GATATCATCT ACCTTTATGA TATGCTATTT ATCCAGCCCA GACTCCAGTT TGTAAGAGGA 
GGAGACATAA TAGTGGATTC AAATGAGCTA AGGAAACACT ACAGGACTTC TACAAAATTT 
CAGTTGGATG TCGCATCAAT AATACCATTT GATATTTGCT ACCTCTTCTT TGGGTTTAAT 
CCAATGTTTA GAGCAAATAG GATGTTAAAG TACACTTCAT TTTTTGAATT TAATCATCAC 
CTAGAGTCTA TAATGGACAA AGCATATATC TACAGAGTTA TTCGAACAAC TGGATACTTG 
CTGTTTATTC TGCACATTAA TGCCTGTGTT TATTACTGGG CTTCAAACTA TGAAGGAATT 
GGCACTACTA GATGGGTGTA TGATGGGGAA GGAAACGAGT ATCTGAGATG TTATTATTGG 
GCAGTTCGAA CTTTAATTAC CATTGGTGGC CTTCCAGAAC CACAAACTTT ATTTGAAATT 
GTTTTTCAAC TCTTGAATTT TTTTTCTGGA GTTTTTGTGT TCTCCAGTTT AATTGGTCAG 
ATGAGAGATG TGATTGGAGC AGCTACAGCC AATCAGAACT ACTTCCGCGC CTGCATGGAT 
GACACCATTG CCTACATGAA CAATTACTCC ATTCCTAAAC TTGTGCAAAA GCGAGTTCGG 
ACTTGGTATG AATATACATG GGACTCTCAA AGAATGCTAG ATGAGTCTGA TTTGCTTAAG 
ACCCTACCAA CTACGGTCCA GTTAGCCCTC GCCATTGATG TGAACTTCAG CATCATCAGC 
AAAGTCGACT TGTTCAAGGG TTGTGATACA CAGATGATTT ATGACATGTT GCTAAGATTG 
AAATCCGTTC TCTATTTGCC TGGTGACTTT GTCTGCAAAA AGGGAGAAAT TGGCAAGGAA 
ATGTATATCA TCAAGCATGG AGAAGTCCAA GTTCTTGGAG GCCCTGATGG TACTAAAGTT 
CTGGTTACTC TGAAAGCTGG GTCGGTGTTT GGAGAAATCA GCCTTCTAGC AGCAGGAGGA 
GGAAACCGTC GAACTGCCAA TGTGGTGGCC CACGGGTTTG CCAATCTTTT AACTCTAGAC 
AAAAAGACCC TCCAAGAAAT TCTAGTGCAT TATCCAGATT CTGAAAGGAT CCTCATGAAG 
AAAGCCAGAG TGCTTTTAAA GCAGAAGGCT AAGACCGCAG AAGCAACCCC TCCAAGAAAA 
GATCTTGCCC TCCTCTTCCC ACCGAAAGAA GAGACACCCA AACTGTTTAA AACTCTCCTA 
GGAGGCACAG GAAAAGCAAG TCTTGCAAGA CTACTCAAAT TGAAGCGAGA GCAAGCAGCT 
CAGAAGAAAG AAAATTCTGA AGGAGGAGAG GAAGAAGGAA AAGAAAATGA AGATAAACAA 
AAAGAAAATG AAGATAAACA AAAAGAAAAT GAAGATAAAG GAAAAGAAAA TGAAGATAAA 
GATAAAGGAA GAGAGCCAGA AGAGAAGCCA CTGGACAGAC CTGAATGTAC AGCAAGTCCT 
ATTGCAGTGG AGGAAGAACC CCACTCAGTT AGAAGGACAG TTTTACCCAG AGGGACTTCT 
CGTCAATCAC TCATTATCAG CATGGCTCCT TCTGCTGAGG GCGGAGAAGA GGTTCTTACT 
ATTGAAGTCA AAGAAAAGGC TAAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNGB3 ( XM_011517138.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011515440.1 Homo sapiens cyclic nucleotide gated channel subunit beta 3 (CNGB3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu68494
Clone ID Related Accession (Same CDS sequence)	XM_011517138.2
Accession Version	XM_011517138.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cyclic nucleotide-gated cation channel beta-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011517138.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTCAAA GAACAGCCCT CTACAAGAAA AAGTTGGTAG AGGGAGATCT CTCCTCACCC 
GAAGCCAGCC CACAAACTGC AAAGCCCACG GCTGTACCAC CAGTAAAAGA AAGCGATGAT 
AAGCCAACAG AACATTACTA CAGGCTGTTG TGGTTCAAAG TCAAAAAGAT GCCTTTAACA 
GAGTACTTAA AGCGAATTAA ACTTCCAAAC AGCATAGATT CATACACAGA TCGACTCTAT 
CTCCTGTGGC TCTTGCTTGT CACTCTTGCC TATAACTGGA ACTGCTGTTT TATACCACTG 
CGCCTCGTCT TCCCATATCA AACCGCAGAC AACATACACT ACTGGCTTAT TGCGGACATC 
ATATGTGATA TCATCTACCT TTATGATATG CTATTTATCC AGCCCAGACT CCAGTTTGTA 
AGAGGAGGAG ACATAATAGT GGATTCAAAT GAGCTAAGGA AACACTACAG GACTTCTACA 
AAATTTCAGT TGGATGTCGC ATCAATAATA CCATTTGATA TTTGCTACCT CTTCTTTGGG 
TTTAATCCAA TGTTTAGAGC AAATAGGATG TTAAAGTACA CTTCATTTTT TGAATTTAAT 
CATCACCTAG AGTCTATAAT GGACAAAGCA TATATCTACA GAGTTATTCG AACAACTGGA 
TACTTGCTGT TTATTCTGCA CATTAATGCC TGTGTTTATT ACTGGGCTTC AAACTATGAA 
GGAATTGGCA CTACTAGATG GGTGTATGAT GGGGAAGGAA ACGAGTATCT GAGATGTTAT 
TATTGGGCAG TTCGAACTTT AATTACCATT GGTGGCCTTC CAGAACCACA AACTTTATTT 
GAAATTGTTT TTCAACTCTT GAATTTTTTT TCTGGAGTTT TTGTGTTCTC CAGTTTAATT 
GGTCAGATGA GAGATGTGAT TGGAGCAGCT ACAGCCAATC AGAACTACTT CCGCGCCTGC 
ATGGATGACA CCATTGCCTA CATGAACAAT TACTCCATTC CTAAACTTGT GCAAAAGCGA 
GTTCGGACTT GGTATGAATA TACATGGGAC TCTCAAAGAA TGCTAGATGA GTCTGATTTG 
CTTAAGACCC TACCAACTAC GGTCCAGTTA GCCCTCGCCA TTGATGTGAA CTTCAGCATC 
ATCAGCAAAG TCGACTTGTT CAAGGGTTGT GATACACAGA TGATTTATGA CATGTTGCTA 
AGATTGAAAT CCGTTCTCTA TTTGCCTGGT GACTTTGTCT GCAAAAAGGG AGAAATTGGC 
AAGGAAATGT ATATCATCAA GCATGGAGAA GTCCAAGTTC TTGGAGGCCC TGATGGTACT 
AAAGTTCTGG TTACTCTGAA AGCTGGGTCG GTGTTTGGAG AAATCAGCCT TCTAGCAGCA 
GGAGGAGGAA ACCGTCGAAC TGCCAATGTG GTGGCCCACG GGTTTGCCAA TCTTTTAACT 
CTAGACAAAA AGACCCTCCA AGAAATTCTA GTGCATTATC CAGATTCTGA AAGGATCCTC 
ATGAAGAAAG CCAGAGTGCT TTTAAAGCAG AAGGCTAAGA CCGCAGAAGC AACCCCTCCA 
AGAAAAGATC TTGCCCTCCT CTTCCCACCG AAAGAAGAGA CACCCAAACT GTTTAAAACT 
CTCCTAGGAG GCACAGGAAA AGCAAGTCTT GCAAGACTAC TCAAATTGAA GCGAGAGCAA 
GCAGCTCAGA AGAAAGAAAA TTCTGAAGGA GGAGAGGAAG AAGGAAAAGA AAATGAAGAT 
AAACAAAAAG AAAATGAAGA TAAACAAAAA GAAAATGAAG ATAAAGGAAA AGAAAATGAA 
GATAAAGATA AAGGAAGAGA GCCAGAAGAG AAGCCACTGG ACAGACCTGA ATGTACAGCA 
AGTCCTATTG CAGTGGAGGA AGAACCCCAC TCAGTTAGAA GGACAGTTTT ACCCAGAGGG 
ACTTCTCGTC AATCACTCAT TATCAGCATG GCTCCTTCTG CTGAGGGCGG AGAAGAGGTT 
CTTACTATTG AAGTCAAAGA AAAGGCTAAG CAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011515440.1
CDS	118..2133
Translation	MRQRTALYKKKLVEGDLSSPEASPQTAKPTAVPPVKESDDKPTEHYYRLLWFKVKKMPLTEYLKRIKLPNSIDSYTDRLYLLWLLLVTLAYNWNCCFIPLRLVFPYQTADNIHYWLIADIICDIIYLYDMLFIQPRLQFVRGGDIIVDSNELRKHYRTSTKFQLDVASIIPFDICYLFFGFNPMFRANRMLKYTSFFEFNHHLESIMDKAYIYRVIRTTGYLLFILHINACVYYWASNYEGIGTTRWVYDGEGNEYLRCYYWAVRTLITIGGLPEPQTLFEIVFQLLNFFSGVFVFSSLIGQMRDVIGAATANQNYFRACMDDTIAYMNNYSIPKLVQKRVRTWYEYTWDSQRMLDESDLLKTLPTTVQLALAIDVNFSIISKVDLFKGCDTQMIYDMLLRLKSVLYLPGDFVCKKGEIGKEMYIIKHGEVQVLGGPDGTKVLVTLKAGSVFGEISLLAAGGGNRRTANVVAHGFANLLTLDKKTLQEILVHYPDSERILMKKARVLLKQKAKTAEATPPRKDLALLFPPKEETPKLFKTLLGGTGKASLARLLKLKREQAAQKKENSEGGEEEGKENEDKQKENEDKQKENEDKGKENEDKDKGREPEEKPLDRPECTASPIAVEEEPHSVRRTVLPRGTSRQSLIISMAPSAEGGEEVLTIEVKEKAKQ

Target ORF information:

RefSeq Version	XM_011517138.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cyclic nucleotide gated channel subunit beta 3 (CNGB3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011517138.2

ORF Insert Sequence:

ATGCGTCAAA GAACAGCCCT CTACAAGAAA AAGTTGGTAG AGGGAGATCT CTCCTCACCC 
GAAGCCAGCC CACAAACTGC AAAGCCCACG GCTGTACCAC CAGTAAAAGA AAGCGATGAT 
AAGCCAACAG AACATTACTA CAGGCTGTTG TGGTTCAAAG TCAAAAAGAT GCCTTTAACA 
GAGTACTTAA AGCGAATTAA ACTTCCAAAC AGCATAGATT CATACACAGA TCGACTCTAT 
CTCCTGTGGC TCTTGCTTGT CACTCTTGCC TATAACTGGA ACTGCTGTTT TATACCACTG 
CGCCTCGTCT TCCCATATCA AACCGCAGAC AACATACACT ACTGGCTTAT TGCGGACATC 
ATATGTGATA TCATCTACCT TTATGATATG CTATTTATCC AGCCCAGACT CCAGTTTGTA 
AGAGGAGGAG ACATAATAGT GGATTCAAAT GAGCTAAGGA AACACTACAG GACTTCTACA 
AAATTTCAGT TGGATGTCGC ATCAATAATA CCATTTGATA TTTGCTACCT CTTCTTTGGG 
TTTAATCCAA TGTTTAGAGC AAATAGGATG TTAAAGTACA CTTCATTTTT TGAATTTAAT 
CATCACCTAG AGTCTATAAT GGACAAAGCA TATATCTACA GAGTTATTCG AACAACTGGA 
TACTTGCTGT TTATTCTGCA CATTAATGCC TGTGTTTATT ACTGGGCTTC AAACTATGAA 
GGAATTGGCA CTACTAGATG GGTGTATGAT GGGGAAGGAA ACGAGTATCT GAGATGTTAT 
TATTGGGCAG TTCGAACTTT AATTACCATT GGTGGCCTTC CAGAACCACA AACTTTATTT 
GAAATTGTTT TTCAACTCTT GAATTTTTTT TCTGGAGTTT TTGTGTTCTC CAGTTTAATT 
GGTCAGATGA GAGATGTGAT TGGAGCAGCT ACAGCCAATC AGAACTACTT CCGCGCCTGC 
ATGGATGACA CCATTGCCTA CATGAACAAT TACTCCATTC CTAAACTTGT GCAAAAGCGA 
GTTCGGACTT GGTATGAATA TACATGGGAC TCTCAAAGAA TGCTAGATGA GTCTGATTTG 
CTTAAGACCC TACCAACTAC GGTCCAGTTA GCCCTCGCCA TTGATGTGAA CTTCAGCATC 
ATCAGCAAAG TCGACTTGTT CAAGGGTTGT GATACACAGA TGATTTATGA CATGTTGCTA 
AGATTGAAAT CCGTTCTCTA TTTGCCTGGT GACTTTGTCT GCAAAAAGGG AGAAATTGGC 
AAGGAAATGT ATATCATCAA GCATGGAGAA GTCCAAGTTC TTGGAGGCCC TGATGGTACT 
AAAGTTCTGG TTACTCTGAA AGCTGGGTCG GTGTTTGGAG AAATCAGCCT TCTAGCAGCA 
GGAGGAGGAA ACCGTCGAAC TGCCAATGTG GTGGCCCACG GGTTTGCCAA TCTTTTAACT 
CTAGACAAAA AGACCCTCCA AGAAATTCTA GTGCATTATC CAGATTCTGA AAGGATCCTC 
ATGAAGAAAG CCAGAGTGCT TTTAAAGCAG AAGGCTAAGA CCGCAGAAGC AACCCCTCCA 
AGAAAAGATC TTGCCCTCCT CTTCCCACCG AAAGAAGAGA CACCCAAACT GTTTAAAACT 
CTCCTAGGAG GCACAGGAAA AGCAAGTCTT GCAAGACTAC TCAAATTGAA GCGAGAGCAA 
GCAGCTCAGA AGAAAGAAAA TTCTGAAGGA GGAGAGGAAG AAGGAAAAGA AAATGAAGAT 
AAACAAAAAG AAAATGAAGA TAAACAAAAA GAAAATGAAG ATAAAGGAAA AGAAAATGAA 
GATAAAGATA AAGGAAGAGA GCCAGAAGAG AAGCCACTGG ACAGACCTGA ATGTACAGCA 
AGTCCTATTG CAGTGGAGGA AGAACCCCAC TCAGTTAGAA GGACAGTTTT ACCCAGAGGG 
ACTTCTCGTC AATCACTCAT TATCAGCATG GCTCCTTCTG CTGAGGGCGG AGAAGAGGTT 
CTTACTATTG AAGTCAAAGA AAAGGCTAAG CAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CNGB3 mutations cause severe rod dysfunction.
Ophthalmic genetics39(1)108-114(2018 Jan-Feb)
Maguire J,McKibbin M,Khan K,Kohl S,Ali M,McKeefry D

Safety and Efficacy of AAV5 Vectors Expressing Human or Canine CNGB3 in CNGB3-Mutant Dogs.
Human gene therapy. Clinical development28(4)197-207(2017 Dec)
Ye GJ,Kom?romy AM,Zeiss C,Calcedo R,Harman CD,Koehl KL,Stewart GA,Iwabe S,Chiodo VA,Hauswirth WW,Aguirre GD,Chulay JD

CNGB3 mutation spectrum including copy number variations in 552 achromatopsia patients.
Human mutation38(11)1579-1591(2017 Nov)
Mayer AK,Van Cauwenbergh C,Rother C,Baumann B,Reuter P,De Baere E,Wissinger B,Kohl S,

REPEATABILITY AND LONGITUDINAL ASSESSMENT OF FOVEAL CONE STRUCTURE IN CNGB3-ASSOCIATED ACHROMATOPSIA.
Retina (Philadelphia, Pa.)37(10)1956-1966(2017 Oct)
Langlo CS,Erker LR,Parker M,Patterson EJ,Higgins BP,Summerfelt P,Razeen MM,Collison FT,Fishman GA,Kay CN,Zhang J,Weleber RG,Yang P,Pennesi ME,Lam BL,Chulay JD,Dubra A,Hauswirth WW,Wilson DJ,Carroll J,

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CNGB3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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