MARCHF5 cDNA ORF clone, Homo sapiens(human)

The following MARCHF5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MARCHF5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu30455 NM_017824.5
Latest version!
Homo sapiens membrane associated ring-CH-type finger 5 (MARCHF5), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$99.50-$139.30
$199.00
OHu44395 XM_011539886.3
Latest version!
Homo sapiens membrane associated ring-CH-type finger 5 (MARCHF5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
View Old Accession Versions >>
OHu30455 NM_017824.4 Homo sapiens membrane associated ring-CH-type finger 5 (MARCH5), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu44395 XM_011539886.2 Homo sapiens membrane associated ring-CH-type finger 5 (MARCH5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu30455
    Clone ID Related Accession (Same CDS sequence) NM_017824.5 , NM_017824.4
    Accession Version NM_017824.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 837bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-01
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase MARCH5
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK000452.1, BC015480.1 and BE393612.1. On Nov 23, 2018 this sequence version replaced NM_017824.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.250655.1, SRR1803617.146891.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000358935.3/ ENSP00000351813.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGCCGGACC AAGCCCTACA GCAGATGCTG GACAGAAGTT GCTGGGTTTG TTTTGCTACT 
    GATGAAGATG ATAGAACAGC TGAATGGGTG AGACCATGCA GGTGCAGAGG ATCTACAAAA
    TGGGTTCACC AGGCCTGTCT ACAACGCTGG GTGGATGAAA AGCAAAGAGG AAACAGTACA
    GCCAGAGTGG CATGTCCTCA GTGCAATGCT GAATACCTAA TAGTTTTTCC AAAATTGGGT
    CCAGTGGTTT ACGTCTTGGA TCTTGCAGAT AGACTGATCT CAAAAGCCTG TCCATTTGCT
    GCAGCAGGAA TAATGGTCGG CTCTATCTAT TGGACAGCTG TGACTTATGG AGCAGTGACA
    GTGATGCAGG TTGTAGGTCA TAAAGAAGGT CTGGATGTTA TGGAGAGAGC TGATCCTTTA
    TTCCTTTTAA TTGGACTTCC TACTATTCCT GTCATGCTGA TATTAGGCAA GATGATTCGC
    TGGGAGGACT ATGTGCTTAG ACTGTGGCGC AAATACTCGA ATAAACTACA AATTTTAAAT
    AGTATATTTC CAGGGATAGG TTGTCCTGTT CCTCGAATTC CAGCTGAGGC CAATCCTTTA
    GCAGATCATG TCTCTGCTAC TCGAATCTTG TGTGGAGCCC TTGTCTTTCC TACTATTGCT
    ACAATAGTTG GTAAATTGAT GTTCAGTAGT GTTAACTCTA ATTTACAAAG GACAATCTTG
    GGTGGAATTG CGTTTGTTGC CATAAAAGGA GCATTTAAAG TTTACTTCAA ACAGCAGCAA
    TATTTACGAC AGGCACACCG CAAAATTCTG AATTATCCAG AACAAGAAGA AGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060294.1
    CDS329..1165
    Translation

    Target ORF information:

    RefSeq Version NM_017824.5
    Organism Homo sapiens(human)
    Definition Homo sapiens membrane associated ring-CH-type finger 5 (MARCHF5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_017824.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGCCGGACC AAGCCCTACA GCAGATGCTG GACAGAAGTT GCTGGGTTTG TTTTGCTACT 
    GATGAAGATG ATAGAACAGC TGAATGGGTG AGACCATGCA GGTGCAGAGG ATCTACAAAA
    TGGGTTCACC AGGCCTGTCT ACAACGCTGG GTGGATGAAA AGCAAAGAGG AAACAGTACA
    GCCAGAGTGG CATGTCCTCA GTGCAATGCT GAATACCTAA TAGTTTTTCC AAAATTGGGT
    CCAGTGGTTT ACGTCTTGGA TCTTGCAGAT AGACTGATCT CAAAAGCCTG TCCATTTGCT
    GCAGCAGGAA TAATGGTCGG CTCTATCTAT TGGACAGCTG TGACTTATGG AGCAGTGACA
    GTGATGCAGG TTGTAGGTCA TAAAGAAGGT CTGGATGTTA TGGAGAGAGC TGATCCTTTA
    TTCCTTTTAA TTGGACTTCC TACTATTCCT GTCATGCTGA TATTAGGCAA GATGATTCGC
    TGGGAGGACT ATGTGCTTAG ACTGTGGCGC AAATACTCGA ATAAACTACA AATTTTAAAT
    AGTATATTTC CAGGGATAGG TTGTCCTGTT CCTCGAATTC CAGCTGAGGC CAATCCTTTA
    GCAGATCATG TCTCTGCTAC TCGAATCTTG TGTGGAGCCC TTGTCTTTCC TACTATTGCT
    ACAATAGTTG GTAAATTGAT GTTCAGTAGT GTTAACTCTA ATTTACAAAG GACAATCTTG
    GGTGGAATTG CGTTTGTTGC CATAAAAGGA GCATTTAAAG TTTACTTCAA ACAGCAGCAA
    TATTTACGAC AGGCACACCG CAAAATTCTG AATTATCCAG AACAAGAAGA AGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu30455
    Clone ID Related Accession (Same CDS sequence) NM_017824.5 , NM_017824.4
    Accession Version NM_017824.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 837bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase MARCH5
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK000452.1, BC015480.1 and BE393612.1. On Feb 3, 2005 this sequence version replaced NM_017824.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC015480.1, SRR1803617.146891.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGCCGGACC AAGCCCTACA GCAGATGCTG GACAGAAGTT GCTGGGTTTG TTTTGCTACT 
    GATGAAGATG ATAGAACAGC TGAATGGGTG AGACCATGCA GGTGCAGAGG ATCTACAAAA
    TGGGTTCACC AGGCCTGTCT ACAACGCTGG GTGGATGAAA AGCAAAGAGG AAACAGTACA
    GCCAGAGTGG CATGTCCTCA GTGCAATGCT GAATACCTAA TAGTTTTTCC AAAATTGGGT
    CCAGTGGTTT ACGTCTTGGA TCTTGCAGAT AGACTGATCT CAAAAGCCTG TCCATTTGCT
    GCAGCAGGAA TAATGGTCGG CTCTATCTAT TGGACAGCTG TGACTTATGG AGCAGTGACA
    GTGATGCAGG TTGTAGGTCA TAAAGAAGGT CTGGATGTTA TGGAGAGAGC TGATCCTTTA
    TTCCTTTTAA TTGGACTTCC TACTATTCCT GTCATGCTGA TATTAGGCAA GATGATTCGC
    TGGGAGGACT ATGTGCTTAG ACTGTGGCGC AAATACTCGA ATAAACTACA AATTTTAAAT
    AGTATATTTC CAGGGATAGG TTGTCCTGTT CCTCGAATTC CAGCTGAGGC CAATCCTTTA
    GCAGATCATG TCTCTGCTAC TCGAATCTTG TGTGGAGCCC TTGTCTTTCC TACTATTGCT
    ACAATAGTTG GTAAATTGAT GTTCAGTAGT GTTAACTCTA ATTTACAAAG GACAATCTTG
    GGTGGAATTG CGTTTGTTGC CATAAAAGGA GCATTTAAAG TTTACTTCAA ACAGCAGCAA
    TATTTACGAC AGGCACACCG CAAAATTCTG AATTATCCAG AACAAGAAGA AGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060294.1
    CDS333..1169
    Translation

    Target ORF information:

    RefSeq Version NM_017824.4
    Organism Homo sapiens(human)
    Definition Homo sapiens membrane associated ring-CH-type finger 5 (MARCH5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_017824.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGCCGGACC AAGCCCTACA GCAGATGCTG GACAGAAGTT GCTGGGTTTG TTTTGCTACT 
    GATGAAGATG ATAGAACAGC TGAATGGGTG AGACCATGCA GGTGCAGAGG ATCTACAAAA
    TGGGTTCACC AGGCCTGTCT ACAACGCTGG GTGGATGAAA AGCAAAGAGG AAACAGTACA
    GCCAGAGTGG CATGTCCTCA GTGCAATGCT GAATACCTAA TAGTTTTTCC AAAATTGGGT
    CCAGTGGTTT ACGTCTTGGA TCTTGCAGAT AGACTGATCT CAAAAGCCTG TCCATTTGCT
    GCAGCAGGAA TAATGGTCGG CTCTATCTAT TGGACAGCTG TGACTTATGG AGCAGTGACA
    GTGATGCAGG TTGTAGGTCA TAAAGAAGGT CTGGATGTTA TGGAGAGAGC TGATCCTTTA
    TTCCTTTTAA TTGGACTTCC TACTATTCCT GTCATGCTGA TATTAGGCAA GATGATTCGC
    TGGGAGGACT ATGTGCTTAG ACTGTGGCGC AAATACTCGA ATAAACTACA AATTTTAAAT
    AGTATATTTC CAGGGATAGG TTGTCCTGTT CCTCGAATTC CAGCTGAGGC CAATCCTTTA
    GCAGATCATG TCTCTGCTAC TCGAATCTTG TGTGGAGCCC TTGTCTTTCC TACTATTGCT
    ACAATAGTTG GTAAATTGAT GTTCAGTAGT GTTAACTCTA ATTTACAAAG GACAATCTTG
    GGTGGAATTG CGTTTGTTGC CATAAAAGGA GCATTTAAAG TTTACTTCAA ACAGCAGCAA
    TATTTACGAC AGGCACACCG CAAAATTCTG AATTATCCAG AACAAGAAGA AGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44395
    Clone ID Related Accession (Same CDS sequence) XM_011539886.2 , XM_011539886.3
    Accession Version XM_011539886.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 615bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase MARCH5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011539886.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGCACAAGC AAAGGGGTCC AGTGGTTTAC GTCTTGGATC TTGCAGATAG ACTGATCTCA 
    AAAGCCTGTC CATTTGCTGC AGCAGGAATA ATGGTCGGCT CTATCTATTG GACAGCTGTG
    ACTTATGGAG CAGTGACAGT GATGCAGGTT GTAGGTCATA AAGAAGGTCT GGATGTTATG
    GAGAGAGCTG ATCCTTTATT CCTTTTAATT GGACTTCCTA CTATTCCTGT CATGCTGATA
    TTAGGCAAGA TGATTCGCTG GGAGGACTAT GTGCTTAGAC TGTGGCGCAA ATACTCGAAT
    AAACTACAAA TTTTAAATAG TATATTTCCA GGGATAGGTT GTCCTGTTCC TCGAATTCCA
    GCTGAGGCCA ATCCTTTAGC AGATCATGTC TCTGCTACTC GAATCTTGTG TGGAGCCCTT
    GTCTTTCCTA CTATTGCTAC AATAGTTGGT AAATTGATGT TCAGTAGTGT TAACTCTAAT
    TTACAAAGGA CAATCTTGGG TGGAATTGCG TTTGTTGCCA TAAAAGGAGC ATTTAAAGTT
    TACTTCAAAC AGCAGCAATA TTTACGACAG GCACACCGCA AAATTCTGAA TTATCCAGAA
    CAAGAAGAAG CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011538188.1
    CDS703..1317
    Translation

    Target ORF information:

    RefSeq Version XM_011539886.2
    Organism Homo sapiens(human)
    Definition Homo sapiens membrane associated ring-CH-type finger 5 (MARCH5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011539886.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGCACAAGC AAAGGGGTCC AGTGGTTTAC GTCTTGGATC TTGCAGATAG ACTGATCTCA 
    AAAGCCTGTC CATTTGCTGC AGCAGGAATA ATGGTCGGCT CTATCTATTG GACAGCTGTG
    ACTTATGGAG CAGTGACAGT GATGCAGGTT GTAGGTCATA AAGAAGGTCT GGATGTTATG
    GAGAGAGCTG ATCCTTTATT CCTTTTAATT GGACTTCCTA CTATTCCTGT CATGCTGATA
    TTAGGCAAGA TGATTCGCTG GGAGGACTAT GTGCTTAGAC TGTGGCGCAA ATACTCGAAT
    AAACTACAAA TTTTAAATAG TATATTTCCA GGGATAGGTT GTCCTGTTCC TCGAATTCCA
    GCTGAGGCCA ATCCTTTAGC AGATCATGTC TCTGCTACTC GAATCTTGTG TGGAGCCCTT
    GTCTTTCCTA CTATTGCTAC AATAGTTGGT AAATTGATGT TCAGTAGTGT TAACTCTAAT
    TTACAAAGGA CAATCTTGGG TGGAATTGCG TTTGTTGCCA TAAAAGGAGC ATTTAAAGTT
    TACTTCAAAC AGCAGCAATA TTTACGACAG GCACACCGCA AAATTCTGAA TTATCCAGAA
    CAAGAAGAAG CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44395
    Clone ID Related Accession (Same CDS sequence) XM_011539886.2 , XM_011539886.3
    Accession Version XM_011539886.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 615bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase MARCH5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011539886.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGCACAAGC AAAGGGGTCC AGTGGTTTAC GTCTTGGATC TTGCAGATAG ACTGATCTCA 
    AAAGCCTGTC CATTTGCTGC AGCAGGAATA ATGGTCGGCT CTATCTATTG GACAGCTGTG
    ACTTATGGAG CAGTGACAGT GATGCAGGTT GTAGGTCATA AAGAAGGTCT GGATGTTATG
    GAGAGAGCTG ATCCTTTATT CCTTTTAATT GGACTTCCTA CTATTCCTGT CATGCTGATA
    TTAGGCAAGA TGATTCGCTG GGAGGACTAT GTGCTTAGAC TGTGGCGCAA ATACTCGAAT
    AAACTACAAA TTTTAAATAG TATATTTCCA GGGATAGGTT GTCCTGTTCC TCGAATTCCA
    GCTGAGGCCA ATCCTTTAGC AGATCATGTC TCTGCTACTC GAATCTTGTG TGGAGCCCTT
    GTCTTTCCTA CTATTGCTAC AATAGTTGGT AAATTGATGT TCAGTAGTGT TAACTCTAAT
    TTACAAAGGA CAATCTTGGG TGGAATTGCG TTTGTTGCCA TAAAAGGAGC ATTTAAAGTT
    TACTTCAAAC AGCAGCAATA TTTACGACAG GCACACCGCA AAATTCTGAA TTATCCAGAA
    CAAGAAGAAG CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011538188.1
    CDS710..1324
    Translation

    Target ORF information:

    RefSeq Version XM_011539886.3
    Organism Homo sapiens(human)
    Definition Homo sapiens membrane associated ring-CH-type finger 5 (MARCHF5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011539886.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGCACAAGC AAAGGGGTCC AGTGGTTTAC GTCTTGGATC TTGCAGATAG ACTGATCTCA 
    AAAGCCTGTC CATTTGCTGC AGCAGGAATA ATGGTCGGCT CTATCTATTG GACAGCTGTG
    ACTTATGGAG CAGTGACAGT GATGCAGGTT GTAGGTCATA AAGAAGGTCT GGATGTTATG
    GAGAGAGCTG ATCCTTTATT CCTTTTAATT GGACTTCCTA CTATTCCTGT CATGCTGATA
    TTAGGCAAGA TGATTCGCTG GGAGGACTAT GTGCTTAGAC TGTGGCGCAA ATACTCGAAT
    AAACTACAAA TTTTAAATAG TATATTTCCA GGGATAGGTT GTCCTGTTCC TCGAATTCCA
    GCTGAGGCCA ATCCTTTAGC AGATCATGTC TCTGCTACTC GAATCTTGTG TGGAGCCCTT
    GTCTTTCCTA CTATTGCTAC AATAGTTGGT AAATTGATGT TCAGTAGTGT TAACTCTAAT
    TTACAAAGGA CAATCTTGGG TGGAATTGCG TTTGTTGCCA TAAAAGGAGC ATTTAAAGTT
    TACTTCAAAC AGCAGCAATA TTTACGACAG GCACACCGCA AAATTCTGAA TTATCCAGAA
    CAAGAAGAAG CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.