P4HTM cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	P4HTM
Entrez Gene ID	54681
Full Name	prolyl 4-hydroxylase, transmembrane
Synonyms	EGLN4,HIFPH4,P4H-TM,PH-4,PH4,PHD4
General protein information	Preferred Names prolyl 4-hydroxylase, transmembrane Names transmembrane prolyl 4-hydroxylase HIF-PH4 HIF-prolyl hydroxylase 4 HPH-4 P4H with transmembrane domain Prolyl hydroxlase domain-containing 4 hypoxia-inducible factor prolyl 4-hydroxylase hypoxia-inducible factor prolyl hydroxylase 4 proline 4-hydroxylase prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p21.31\|3p21.3
Summary	The product of this gene belongs to the family of prolyl 4-hydroxylases. This protein is a prolyl hydroxylase that may be involved in the degradation of hypoxia-inducible transcription factors under normoxia. It plays a role in adaptation to hypoxia and may be related to cellular oxygen sensing. Alternatively spliced variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008].
Disorder MIM:	614584

mRNA and Protein(s)

mRNA	Protein	Name
NM_177939.2	NP_808808.1	transmembrane prolyl 4-hydroxylase isoform a
NM_177938.2	NP_808807.2	transmembrane prolyl 4-hydroxylase isoform c
NM_177939.3	NP_808808.1	transmembrane prolyl 4-hydroxylase isoform a

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	P4HTM	XP_003432873.1
Mus musculus (house mouse)	P4htm	NP_083220.3
Danio rerio (zebrafish)	LOC799930	XP_001340234.2
Homo sapiens (human)	P4HTM	NP_808808.1
Gallus gallus (chicken)	P4HTM	XP_003642061.1
Xenopus tropicalis (tropical clawed frog)	p4htm	XP_002933103.2
Rattus norvegicus (Norway rat)	P4htm	XP_217285.6
Pan troglodytes (chimpanzee)	P4HTM	XP_001160922.1
Bos taurus (cattle)	P4HTM	NP_001069583.1

Gene Ontology
Bibliography

Related articles in PubMed

Sestrin2 inhibits hypoxia-inducible factor-1? accumulation via AMPK-mediated prolyl hydroxylase regulation.
Seo K, Seo S, Ki SH, Shin SM
Free radical biology & medicine101511-523(2016 Dec)

The hypoxia-inducible factor pathway, prolyl hydroxylase domain protein inhibitors, and their roles in bone repair and regeneration.
Fan L, Li J, Yu Z, Dang X, Wang K
BioMed research international2014239356(2014)

Human prolyl hydroxylase expression in uterine leiomyoma during the menstrual cycle.
Iwahashi M, Muragaki Y, Ino K
Reproductive biology and endocrinology : RB&E10111(2012 Dec)

Transmembrane prolyl 4-hydroxylase is a fourth prolyl 4-hydroxylase regulating EPO production and erythropoiesis.
Laitala A, Aro E, Walkinshaw G, M?ki JM, Rossi M, Heikkil? M, Savolainen ER, Arend M, Kivirikko KI, Koivunen P, Myllyharju J
Blood120(16)3336-44(2012 Oct)

An endoplasmic reticulum transmembrane prolyl 4-hydroxylase is induced by hypoxia and acts on hypoxia-inducible factor alpha.
Koivunen P, Tiainen P, Hyv?rinen J, Williams KE, Sormunen R, Klaus SJ, Kivirikko KI, Myllyharju J
The Journal of biological chemistry282(42)30544-52(2007 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results suggest that SESN2 increases degradation of HIF-1A via AMPK-PHD regulation that contributes to inhibition of in vitro and in vivo tumorigenesis.
Title: Sestrin2 inhibits hypoxia-inducible factor-1? accumulation via AMPK-mediated prolyl hydroxylase regulation.

This review will expand our knowledge of biology of HIFs, PHDs, PHD inhibitors, and bone regeneration, and it may also aid the design of novel therapies for accelerating bone repair and regeneration or inhibiting bone tumours. [review]
Title: The hypoxia-inducible factor pathway, prolyl hydroxylase domain protein inhibitors, and their roles in bone repair and regeneration.

Results suggest that increased expression of prolyl hydroxylase (PH) might play an role in the physiology of uterine leiomyoma during the menstrual cycle.
Title: Human prolyl hydroxylase expression in uterine leiomyoma during the menstrual cycle.

This review summarizes recent progress in elucidating the molecular mechanisms of hypoxia-inducible factor (HIF)-1 activation, focusing on the role of oxygen-dependent asparaginyl hydroxylase in hypoxia signal transduction.
Title: Regulation of hypoxia-inducible factor 1 by prolyl and asparaginyl hydroxylases.

crystal structure of the peptide-substrate-binding domain
Title: The peptide-substrate-binding domain of collagen prolyl 4-hydroxylases is a tetratricopeptide repeat domain with functional aromatic residues.

The following P4HTM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the P4HTM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu29443	NM_177938.2 Latest version!	Homo sapiens prolyl 4-hydroxylase, transmembrane (P4HTM), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$239.50-$335.30 $479.00
OHu29368	NM_177939.3 Latest version!	Homo sapiens prolyl 4-hydroxylase, transmembrane (P4HTM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
View Old Accession Versions >>
OHu29368	NM_177939.2	Homo sapiens prolyl 4-hydroxylase, transmembrane (P4HTM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

P4HTM ( NM_177938.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_808807.2 Homo sapiens prolyl 4-hydroxylase, transmembrane (P4HTM), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu29443
Clone ID Related Accession (Same CDS sequence)	NM_177938.2
Accession Version	NM_177938.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-18
Organism	Homo sapiens(human)
Product	transmembrane prolyl 4-hydroxylase isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC047566.1, BC011710.2, BC000580.1 and AW072069.1. On May 13, 2005 this sequence version replaced NM_177938.1. Transcript Variant: This variant (3) contains two additional segments in the coding region, compared to variant 1. The resulting isoform (c) is longer, compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: BC011710.2, SRR1803614.66591.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCAG CGGCGGTGAC AGGCCAGCGG CCTGAGACCG CGGCGGCCGA GGAGGCCTCG 
AGGCCGCAGT GGGCGCCGCC AGACCACTGC CAGGCTCAGG CGGCGGCCGG GCTGGGCGAC 
GGCGAGGACG CACCGGTGCG TCCGCTGTGC AAGCCCCGCG GCATCTGCTC GCGCGCCTAC 
TTCCTGGTGC TGATGGTGTT CGTGCACCTG TACCTGGGTA ACGTGCTGGC GCTGCTGCTC 
TTCGTGCACT ACAGCAACGG CGACGAAAGC AGCGATCCCG GGCCCCAACA CCGTGCCCAG 
GGCCCCGGGC CCGAGCCCAC CTTAGGTCCC CTCACCCGGC TGGAGGGCAT CAAGGTGGGG 
CACGAGCGTA AGGTCCAGCT GGTCACCGAC AGGGATCACT TCATCCGAAC CCTCAGCCTC 
AAGCCGCTGC TCTTCGAAAT CCCCGGCTTC CTGACTGATG AAGAGTGTCG GCTCATCATC 
CATCTGGCGC AGATGAAGGG GTTACAGCGC AGCCAGATCC TGCCTACTGA AGAGTATGAA 
GAGGCAATGA GCACTATGCA GGTCAGCCAG CTGGACCTCT TCCGGCTGCT GGACCAGAAC 
CGTGATGGGC ACCTTCAGCT CCGTGAGGTT CTGGCCCAGA CTCGCCTGGG AAATGGATGG 
TGGATGACTC CAGAGAGCAT TCAGGAGATG TACGCCGCGA TCAAGGCTGA CCCTGATGGT 
GACGGAGTGC TGAGTCTGCA GGAGTTCTCC AACATGGACC TTCGGGACTT CCACAAGTAC 
ATGAGGAGCC ACAAGGCAGA GTCCAGTGAG CTGGTGCGGA ACAGCCACCA TACCTGGCTC 
TACCAGGGTG AGGGTGCCCA CCACATCATG CGTGCCATCC GCCAGAGGGT GCTGCGCCTC 
ACTCGCCTGT CGCCTGAGAT CGTGGAGCTC AGCGAGCCGC TGCAGGTTGT TCGATATGGT 
GAGGGGGGCC ACTACCATGC CCACGTGGAC AGTGGGCCTG TGTACCCAGA GACCATCTGC 
TCCCATACCA AGCTGGTAGC CAACGAGTCT GTACCCTTCG AGACCTCCTG CCGGCAAGTA 
TCTCCCAACT GGGGGCTGCC TTCAATCCTC AGACCAGGAA CACCCATGAC ACAGGCACAG 
CCCTGCACTG TGGGCGTGCC CCTTGGCATG GGGCCAGGAG ATCACTGGGT TATCCCGGTA 
AGCCCCTGGG AGCATCCACA ACTGGGGACC TGCTCAGTGC CCCCCCTGCC TTACAGCTAC 
ATGACAGTGC TGTTTTATTT GAACAACGTC ACTGGTGGGG GCGAGACTGT TTTCCCTGTA 
GCAGATAACA GAACCTACGA TGAAATGAGT CTGATTCAGG ATGACGTGGA CCTCCGTGAC 
ACACGGAGGC ACTGTGACAA GGGAAACCTG CGTGTCAAGC CCCAACAGGG CACAGCAGTC 
TTCTGGTACA ACTACCTGCC TGATGGGCAA GGTTGGGTGG GTGACGTAGA CGACTACTCG 
CTGCACGGGG GCTGCCTGGT CACGCGCGGC ACCAAGTGGA TTGCCAACAA CTGGATTAAT 
GTGGACCCCA GCCGAGCGCG GCAAGCGCTG TTCCAACAGG AGATGGCCCG CCTTGCCCGA 
GAAGGGGGCA CCGACTCACA GCCCGAGTGG GCTCTGGACC GGGCCTACCG CGATGCGCGC 
GTGGAACTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808807.2
CDS	350..2041
Translation	MAAAAVTGQRPETAAAEEASRPQWAPPDHCQAQAAAGLGDGEDAPVRPLCKPRGICSRAYFLVLMVFVHLYLGNVLALLLFVHYSNGDESSDPGPQHRAQGPGPEPTLGPLTRLEGIKVGHERKVQLVTDRDHFIRTLSLKPLLFEIPGFLTDEECRLIIHLAQMKGLQRSQILPTEEYEEAMSTMQVSQLDLFRLLDQNRDGHLQLREVLAQTRLGNGWWMTPESIQEMYAAIKADPDGDGVLSLQEFSNMDLRDFHKYMRSHKAESSELVRNSHHTWLYQGEGAHHIMRAIRQRVLRLTRLSPEIVELSEPLQVVRYGEGGHYHAHVDSGPVYPETICSHTKLVANESVPFETSCRQVSPNWGLPSILRPGTPMTQAQPCTVGVPLGMGPGDHWVIPVSPWEHPQLGTCSVPPLPYSYMTVLFYLNNVTGGGETVFPVADNRTYDEMSLIQDDVDLRDTRRHCDKGNLRVKPQQGTAVFWYNYLPDGQGWVGDVDDYSLHGGCLVTRGTKWIANNWINVDPSRARQALFQQEMARLAREGGTDSQPEWALDRAYRDARVEL

Target ORF information:

RefSeq Version	NM_177938.2
Organism	Homo sapiens(human)
Definition	Homo sapiens prolyl 4-hydroxylase, transmembrane (P4HTM), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177938.2

ORF Insert Sequence:

ATGGCGGCAG CGGCGGTGAC AGGCCAGCGG CCTGAGACCG CGGCGGCCGA GGAGGCCTCG 
AGGCCGCAGT GGGCGCCGCC AGACCACTGC CAGGCTCAGG CGGCGGCCGG GCTGGGCGAC 
GGCGAGGACG CACCGGTGCG TCCGCTGTGC AAGCCCCGCG GCATCTGCTC GCGCGCCTAC 
TTCCTGGTGC TGATGGTGTT CGTGCACCTG TACCTGGGTA ACGTGCTGGC GCTGCTGCTC 
TTCGTGCACT ACAGCAACGG CGACGAAAGC AGCGATCCCG GGCCCCAACA CCGTGCCCAG 
GGCCCCGGGC CCGAGCCCAC CTTAGGTCCC CTCACCCGGC TGGAGGGCAT CAAGGTGGGG 
CACGAGCGTA AGGTCCAGCT GGTCACCGAC AGGGATCACT TCATCCGAAC CCTCAGCCTC 
AAGCCGCTGC TCTTCGAAAT CCCCGGCTTC CTGACTGATG AAGAGTGTCG GCTCATCATC 
CATCTGGCGC AGATGAAGGG GTTACAGCGC AGCCAGATCC TGCCTACTGA AGAGTATGAA 
GAGGCAATGA GCACTATGCA GGTCAGCCAG CTGGACCTCT TCCGGCTGCT GGACCAGAAC 
CGTGATGGGC ACCTTCAGCT CCGTGAGGTT CTGGCCCAGA CTCGCCTGGG AAATGGATGG 
TGGATGACTC CAGAGAGCAT TCAGGAGATG TACGCCGCGA TCAAGGCTGA CCCTGATGGT 
GACGGAGTGC TGAGTCTGCA GGAGTTCTCC AACATGGACC TTCGGGACTT CCACAAGTAC 
ATGAGGAGCC ACAAGGCAGA GTCCAGTGAG CTGGTGCGGA ACAGCCACCA TACCTGGCTC 
TACCAGGGTG AGGGTGCCCA CCACATCATG CGTGCCATCC GCCAGAGGGT GCTGCGCCTC 
ACTCGCCTGT CGCCTGAGAT CGTGGAGCTC AGCGAGCCGC TGCAGGTTGT TCGATATGGT 
GAGGGGGGCC ACTACCATGC CCACGTGGAC AGTGGGCCTG TGTACCCAGA GACCATCTGC 
TCCCATACCA AGCTGGTAGC CAACGAGTCT GTACCCTTCG AGACCTCCTG CCGGCAAGTA 
TCTCCCAACT GGGGGCTGCC TTCAATCCTC AGACCAGGAA CACCCATGAC ACAGGCACAG 
CCCTGCACTG TGGGCGTGCC CCTTGGCATG GGGCCAGGAG ATCACTGGGT TATCCCGGTA 
AGCCCCTGGG AGCATCCACA ACTGGGGACC TGCTCAGTGC CCCCCCTGCC TTACAGCTAC 
ATGACAGTGC TGTTTTATTT GAACAACGTC ACTGGTGGGG GCGAGACTGT TTTCCCTGTA 
GCAGATAACA GAACCTACGA TGAAATGAGT CTGATTCAGG ATGACGTGGA CCTCCGTGAC 
ACACGGAGGC ACTGTGACAA GGGAAACCTG CGTGTCAAGC CCCAACAGGG CACAGCAGTC 
TTCTGGTACA ACTACCTGCC TGATGGGCAA GGTTGGGTGG GTGACGTAGA CGACTACTCG 
CTGCACGGGG GCTGCCTGGT CACGCGCGGC ACCAAGTGGA TTGCCAACAA CTGGATTAAT 
GTGGACCCCA GCCGAGCGCG GCAAGCGCTG TTCCAACAGG AGATGGCCCG CCTTGCCCGA 
GAAGGGGGCA CCGACTCACA GCCCGAGTGG GCTCTGGACC GGGCCTACCG CGATGCGCGC 
GTGGAACTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P4HTM ( NM_177939.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_808808.1 Homo sapiens prolyl 4-hydroxylase, transmembrane (P4HTM), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu29368
Clone ID Related Accession (Same CDS sequence)	NM_177939.2 , NM_177939.3
Accession Version	NM_177939.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-31
Organism	Homo sapiens(human)
Product	transmembrane prolyl 4-hydroxylase isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC047566.1, BC011710.2, BC000580.1 and AW072069.1. On or before Sep 20, 2006 this sequence version replaced XM_001133930.1, XM_001133929.1, NM_177939.1. Transcript Variant: This variant (1) represents the shortest transcript. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.43044.1, SRR1660805.189045.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCAG CGGCGGTGAC AGGCCAGCGG CCTGAGACCG CGGCGGCCGA GGAGGCCTCG 
AGGCCGCAGT GGGCGCCGCC AGACCACTGC CAGGCTCAGG CGGCGGCCGG GCTGGGCGAC 
GGCGAGGACG CACCGGTGCG TCCGCTGTGC AAGCCCCGCG GCATCTGCTC GCGCGCCTAC 
TTCCTGGTGC TGATGGTGTT CGTGCACCTG TACCTGGGTA ACGTGCTGGC GCTGCTGCTC 
TTCGTGCACT ACAGCAACGG CGACGAAAGC AGCGATCCCG GGCCCCAACA CCGTGCCCAG 
GGCCCCGGGC CCGAGCCCAC CTTAGGTCCC CTCACCCGGC TGGAGGGCAT CAAGGTGGGG 
CACGAGCGTA AGGTCCAGCT GGTCACCGAC AGGGATCACT TCATCCGAAC CCTCAGCCTC 
AAGCCGCTGC TCTTCGAAAT CCCCGGCTTC CTGACTGATG AAGAGTGTCG GCTCATCATC 
CATCTGGCGC AGATGAAGGG GTTACAGCGC AGCCAGATCC TGCCTACTGA AGAGTATGAA 
GAGGCAATGA GCACTATGCA GGTCAGCCAG CTGGACCTCT TCCGGCTGCT GGACCAGAAC 
CGTGATGGGC ACCTTCAGCT CCGTGAGGTT CTGGCCCAGA CTCGCCTGGG AAATGGATGG 
TGGATGACTC CAGAGAGCAT TCAGGAGATG TACGCCGCGA TCAAGGCTGA CCCTGATGGT 
GACGGAGTGC TGAGTCTGCA GGAGTTCTCC AACATGGACC TTCGGGACTT CCACAAGTAC 
ATGAGGAGCC ACAAGGCAGA GTCCAGTGAG CTGGTGCGGA ACAGCCACCA TACCTGGCTC 
TACCAGGGTG AGGGTGCCCA CCACATCATG CGTGCCATCC GCCAGAGGGT GCTGCGCCTC 
ACTCGCCTGT CGCCTGAGAT CGTGGAGCTC AGCGAGCCGC TGCAGGTTGT TCGATATGGT 
GAGGGGGGCC ACTACCATGC CCACGTGGAC AGTGGGCCTG TGTACCCAGA GACCATCTGC 
TCCCATACCA AGCTGGTAGC CAACGAGTCT GTACCCTTCG AGACCTCCTG CCGCTACATG 
ACAGTGCTGT TTTATTTGAA CAACGTCACT GGTGGGGGCG AGACTGTTTT CCCTGTAGCA 
GATAACAGAA CCTACGATGA AATGAGTCTG ATTCAGGATG ACGTGGACCT CCGTGACACA 
CGGAGGCACT GTGACAAGGG AAACCTGCGT GTCAAGCCCC AACAGGGCAC AGCAGTCTTC 
TGGTACAACT ACCTGCCTGA TGGGCAAGGT TGGGTGGGTG ACGTAGACGA CTACTCGCTG 
CACGGGGGCT GCCTGGTCAC GCGCGGCACC AAGTGGATTG CCAACAACTG GATTAATGTG 
GACCCCAGCC GAGCGCGGCA AGCGCTGTTC CAACAGGAGA TGGCCCGCCT TGCCCGAGAA 
GGGGGCACCG ACTCACAGCC CGAGTGGGCT CTGGACCGGG CCTACCGCGA TGCGCGCGTG 
GAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808808.1
CDS	350..1858
Translation	MAAAAVTGQRPETAAAEEASRPQWAPPDHCQAQAAAGLGDGEDAPVRPLCKPRGICSRAYFLVLMVFVHLYLGNVLALLLFVHYSNGDESSDPGPQHRAQGPGPEPTLGPLTRLEGIKVGHERKVQLVTDRDHFIRTLSLKPLLFEIPGFLTDEECRLIIHLAQMKGLQRSQILPTEEYEEAMSTMQVSQLDLFRLLDQNRDGHLQLREVLAQTRLGNGWWMTPESIQEMYAAIKADPDGDGVLSLQEFSNMDLRDFHKYMRSHKAESSELVRNSHHTWLYQGEGAHHIMRAIRQRVLRLTRLSPEIVELSEPLQVVRYGEGGHYHAHVDSGPVYPETICSHTKLVANESVPFETSCRYMTVLFYLNNVTGGGETVFPVADNRTYDEMSLIQDDVDLRDTRRHCDKGNLRVKPQQGTAVFWYNYLPDGQGWVGDVDDYSLHGGCLVTRGTKWIANNWINVDPSRARQALFQQEMARLAREGGTDSQPEWALDRAYRDARVEL

Target ORF information:

RefSeq Version	NM_177939.2
Organism	Homo sapiens(human)
Definition	Homo sapiens prolyl 4-hydroxylase, transmembrane (P4HTM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177939.2

ORF Insert Sequence:

ATGGCGGCAG CGGCGGTGAC AGGCCAGCGG CCTGAGACCG CGGCGGCCGA GGAGGCCTCG 
AGGCCGCAGT GGGCGCCGCC AGACCACTGC CAGGCTCAGG CGGCGGCCGG GCTGGGCGAC 
GGCGAGGACG CACCGGTGCG TCCGCTGTGC AAGCCCCGCG GCATCTGCTC GCGCGCCTAC 
TTCCTGGTGC TGATGGTGTT CGTGCACCTG TACCTGGGTA ACGTGCTGGC GCTGCTGCTC 
TTCGTGCACT ACAGCAACGG CGACGAAAGC AGCGATCCCG GGCCCCAACA CCGTGCCCAG 
GGCCCCGGGC CCGAGCCCAC CTTAGGTCCC CTCACCCGGC TGGAGGGCAT CAAGGTGGGG 
CACGAGCGTA AGGTCCAGCT GGTCACCGAC AGGGATCACT TCATCCGAAC CCTCAGCCTC 
AAGCCGCTGC TCTTCGAAAT CCCCGGCTTC CTGACTGATG AAGAGTGTCG GCTCATCATC 
CATCTGGCGC AGATGAAGGG GTTACAGCGC AGCCAGATCC TGCCTACTGA AGAGTATGAA 
GAGGCAATGA GCACTATGCA GGTCAGCCAG CTGGACCTCT TCCGGCTGCT GGACCAGAAC 
CGTGATGGGC ACCTTCAGCT CCGTGAGGTT CTGGCCCAGA CTCGCCTGGG AAATGGATGG 
TGGATGACTC CAGAGAGCAT TCAGGAGATG TACGCCGCGA TCAAGGCTGA CCCTGATGGT 
GACGGAGTGC TGAGTCTGCA GGAGTTCTCC AACATGGACC TTCGGGACTT CCACAAGTAC 
ATGAGGAGCC ACAAGGCAGA GTCCAGTGAG CTGGTGCGGA ACAGCCACCA TACCTGGCTC 
TACCAGGGTG AGGGTGCCCA CCACATCATG CGTGCCATCC GCCAGAGGGT GCTGCGCCTC 
ACTCGCCTGT CGCCTGAGAT CGTGGAGCTC AGCGAGCCGC TGCAGGTTGT TCGATATGGT 
GAGGGGGGCC ACTACCATGC CCACGTGGAC AGTGGGCCTG TGTACCCAGA GACCATCTGC 
TCCCATACCA AGCTGGTAGC CAACGAGTCT GTACCCTTCG AGACCTCCTG CCGCTACATG 
ACAGTGCTGT TTTATTTGAA CAACGTCACT GGTGGGGGCG AGACTGTTTT CCCTGTAGCA 
GATAACAGAA CCTACGATGA AATGAGTCTG ATTCAGGATG ACGTGGACCT CCGTGACACA 
CGGAGGCACT GTGACAAGGG AAACCTGCGT GTCAAGCCCC AACAGGGCAC AGCAGTCTTC 
TGGTACAACT ACCTGCCTGA TGGGCAAGGT TGGGTGGGTG ACGTAGACGA CTACTCGCTG 
CACGGGGGCT GCCTGGTCAC GCGCGGCACC AAGTGGATTG CCAACAACTG GATTAATGTG 
GACCCCAGCC GAGCGCGGCA AGCGCTGTTC CAACAGGAGA TGGCCCGCCT TGCCCGAGAA 
GGGGGCACCG ACTCACAGCC CGAGTGGGCT CTGGACCGGG CCTACCGCGA TGCGCGCGTG 
GAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P4HTM ( NM_177939.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_808808.1 Homo sapiens prolyl 4-hydroxylase, transmembrane (P4HTM), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu29368
Clone ID Related Accession (Same CDS sequence)	NM_177939.2 , NM_177939.3
Accession Version	NM_177939.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-18
Organism	Homo sapiens(human)
Product	transmembrane prolyl 4-hydroxylase isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC047566.1, BC011710.2, BC000580.1, AW072069.1 and AC137630.9. On Nov 23, 2018 this sequence version replaced NM_177939.2. Transcript Variant: This variant (1) represents the shortest transcript. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.80976.1, SRR3476690.955576.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000383729.9/ ENSP00000373235.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCAG CGGCGGTGAC AGGCCAGCGG CCTGAGACCG CGGCGGCCGA GGAGGCCTCG 
AGGCCGCAGT GGGCGCCGCC AGACCACTGC CAGGCTCAGG CGGCGGCCGG GCTGGGCGAC 
GGCGAGGACG CACCGGTGCG TCCGCTGTGC AAGCCCCGCG GCATCTGCTC GCGCGCCTAC 
TTCCTGGTGC TGATGGTGTT CGTGCACCTG TACCTGGGTA ACGTGCTGGC GCTGCTGCTC 
TTCGTGCACT ACAGCAACGG CGACGAAAGC AGCGATCCCG GGCCCCAACA CCGTGCCCAG 
GGCCCCGGGC CCGAGCCCAC CTTAGGTCCC CTCACCCGGC TGGAGGGCAT CAAGGTGGGG 
CACGAGCGTA AGGTCCAGCT GGTCACCGAC AGGGATCACT TCATCCGAAC CCTCAGCCTC 
AAGCCGCTGC TCTTCGAAAT CCCCGGCTTC CTGACTGATG AAGAGTGTCG GCTCATCATC 
CATCTGGCGC AGATGAAGGG GTTACAGCGC AGCCAGATCC TGCCTACTGA AGAGTATGAA 
GAGGCAATGA GCACTATGCA GGTCAGCCAG CTGGACCTCT TCCGGCTGCT GGACCAGAAC 
CGTGATGGGC ACCTTCAGCT CCGTGAGGTT CTGGCCCAGA CTCGCCTGGG AAATGGATGG 
TGGATGACTC CAGAGAGCAT TCAGGAGATG TACGCCGCGA TCAAGGCTGA CCCTGATGGT 
GACGGAGTGC TGAGTCTGCA GGAGTTCTCC AACATGGACC TTCGGGACTT CCACAAGTAC 
ATGAGGAGCC ACAAGGCAGA GTCCAGTGAG CTGGTGCGGA ACAGCCACCA TACCTGGCTC 
TACCAGGGTG AGGGTGCCCA CCACATCATG CGTGCCATCC GCCAGAGGGT GCTGCGCCTC 
ACTCGCCTGT CGCCTGAGAT CGTGGAGCTC AGCGAGCCGC TGCAGGTTGT TCGATATGGT 
GAGGGGGGCC ACTACCATGC CCACGTGGAC AGTGGGCCTG TGTACCCAGA GACCATCTGC 
TCCCATACCA AGCTGGTAGC CAACGAGTCT GTACCCTTCG AGACCTCCTG CCGCTACATG 
ACAGTGCTGT TTTATTTGAA CAACGTCACT GGTGGGGGCG AGACTGTTTT CCCTGTAGCA 
GATAACAGAA CCTACGATGA AATGAGTCTG ATTCAGGATG ACGTGGACCT CCGTGACACA 
CGGAGGCACT GTGACAAGGG AAACCTGCGT GTCAAGCCCC AACAGGGCAC AGCAGTCTTC 
TGGTACAACT ACCTGCCTGA TGGGCAAGGT TGGGTGGGTG ACGTAGACGA CTACTCGCTG 
CACGGGGGCT GCCTGGTCAC GCGCGGCACC AAGTGGATTG CCAACAACTG GATTAATGTG 
GACCCCAGCC GAGCGCGGCA AGCGCTGTTC CAACAGGAGA TGGCCCGCCT TGCCCGAGAA 
GGGGGCACCG ACTCACAGCC CGAGTGGGCT CTGGACCGGG CCTACCGCGA TGCGCGCGTG 
GAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808808.1
CDS	17..1525
Translation	MAAAAVTGQRPETAAAEEASRPQWAPPDHCQAQAAAGLGDGEDAPVRPLCKPRGICSRAYFLVLMVFVHLYLGNVLALLLFVHYSNGDESSDPGPQHRAQGPGPEPTLGPLTRLEGIKVGHERKVQLVTDRDHFIRTLSLKPLLFEIPGFLTDEECRLIIHLAQMKGLQRSQILPTEEYEEAMSTMQVSQLDLFRLLDQNRDGHLQLREVLAQTRLGNGWWMTPESIQEMYAAIKADPDGDGVLSLQEFSNMDLRDFHKYMRSHKAESSELVRNSHHTWLYQGEGAHHIMRAIRQRVLRLTRLSPEIVELSEPLQVVRYGEGGHYHAHVDSGPVYPETICSHTKLVANESVPFETSCRYMTVLFYLNNVTGGGETVFPVADNRTYDEMSLIQDDVDLRDTRRHCDKGNLRVKPQQGTAVFWYNYLPDGQGWVGDVDDYSLHGGCLVTRGTKWIANNWINVDPSRARQALFQQEMARLAREGGTDSQPEWALDRAYRDARVEL

Target ORF information:

RefSeq Version	NM_177939.3
Organism	Homo sapiens(human)
Definition	Homo sapiens prolyl 4-hydroxylase, transmembrane (P4HTM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177939.3

ORF Insert Sequence:

ATGGCGGCAG CGGCGGTGAC AGGCCAGCGG CCTGAGACCG CGGCGGCCGA GGAGGCCTCG 
AGGCCGCAGT GGGCGCCGCC AGACCACTGC CAGGCTCAGG CGGCGGCCGG GCTGGGCGAC 
GGCGAGGACG CACCGGTGCG TCCGCTGTGC AAGCCCCGCG GCATCTGCTC GCGCGCCTAC 
TTCCTGGTGC TGATGGTGTT CGTGCACCTG TACCTGGGTA ACGTGCTGGC GCTGCTGCTC 
TTCGTGCACT ACAGCAACGG CGACGAAAGC AGCGATCCCG GGCCCCAACA CCGTGCCCAG 
GGCCCCGGGC CCGAGCCCAC CTTAGGTCCC CTCACCCGGC TGGAGGGCAT CAAGGTGGGG 
CACGAGCGTA AGGTCCAGCT GGTCACCGAC AGGGATCACT TCATCCGAAC CCTCAGCCTC 
AAGCCGCTGC TCTTCGAAAT CCCCGGCTTC CTGACTGATG AAGAGTGTCG GCTCATCATC 
CATCTGGCGC AGATGAAGGG GTTACAGCGC AGCCAGATCC TGCCTACTGA AGAGTATGAA 
GAGGCAATGA GCACTATGCA GGTCAGCCAG CTGGACCTCT TCCGGCTGCT GGACCAGAAC 
CGTGATGGGC ACCTTCAGCT CCGTGAGGTT CTGGCCCAGA CTCGCCTGGG AAATGGATGG 
TGGATGACTC CAGAGAGCAT TCAGGAGATG TACGCCGCGA TCAAGGCTGA CCCTGATGGT 
GACGGAGTGC TGAGTCTGCA GGAGTTCTCC AACATGGACC TTCGGGACTT CCACAAGTAC 
ATGAGGAGCC ACAAGGCAGA GTCCAGTGAG CTGGTGCGGA ACAGCCACCA TACCTGGCTC 
TACCAGGGTG AGGGTGCCCA CCACATCATG CGTGCCATCC GCCAGAGGGT GCTGCGCCTC 
ACTCGCCTGT CGCCTGAGAT CGTGGAGCTC AGCGAGCCGC TGCAGGTTGT TCGATATGGT 
GAGGGGGGCC ACTACCATGC CCACGTGGAC AGTGGGCCTG TGTACCCAGA GACCATCTGC 
TCCCATACCA AGCTGGTAGC CAACGAGTCT GTACCCTTCG AGACCTCCTG CCGCTACATG 
ACAGTGCTGT TTTATTTGAA CAACGTCACT GGTGGGGGCG AGACTGTTTT CCCTGTAGCA 
GATAACAGAA CCTACGATGA AATGAGTCTG ATTCAGGATG ACGTGGACCT CCGTGACACA 
CGGAGGCACT GTGACAAGGG AAACCTGCGT GTCAAGCCCC AACAGGGCAC AGCAGTCTTC 
TGGTACAACT ACCTGCCTGA TGGGCAAGGT TGGGTGGGTG ACGTAGACGA CTACTCGCTG 
CACGGGGGCT GCCTGGTCAC GCGCGGCACC AAGTGGATTG CCAACAACTG GATTAATGTG 
GACCCCAGCC GAGCGCGGCA AGCGCTGTTC CAACAGGAGA TGGCCCGCCT TGCCCGAGAA 
GGGGGCACCG ACTCACAGCC CGAGTGGGCT CTGGACCGGG CCTACCGCGA TGCGCGCGTG 
GAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sestrin2 inhibits hypoxia-inducible factor-1? accumulation via AMPK-mediated prolyl hydroxylase regulation.
Free radical biology & medicine101511-523(2016 Dec)
Seo K,Seo S,Ki SH,Shin SM

The hypoxia-inducible factor pathway, prolyl hydroxylase domain protein inhibitors, and their roles in bone repair and regeneration.
BioMed research international2014239356(2014)
Fan L,Li J,Yu Z,Dang X,Wang K

Human prolyl hydroxylase expression in uterine leiomyoma during the menstrual cycle.
Reproductive biology and endocrinology : RB&E10111(2012 Dec)
Iwahashi M,Muragaki Y,Ino K

Transmembrane prolyl 4-hydroxylase is a fourth prolyl 4-hydroxylase regulating EPO production and erythropoiesis.
Blood120(16)3336-44(2012 Oct)
Laitala A,Aro E,Walkinshaw G,M?ki JM,Rossi M,Heikkil? M,Savolainen ER,Arend M,Kivirikko KI,Koivunen P,Myllyharju J

An endoplasmic reticulum transmembrane prolyl 4-hydroxylase is induced by hypoxia and acts on hypoxia-inducible factor alpha.
The Journal of biological chemistry282(42)30544-52(2007 Oct)
Koivunen P,Tiainen P,Hyv?rinen J,Williams KE,Sormunen R,Klaus SJ,Kivirikko KI,Myllyharju J

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P4HTM cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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