POU4F2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	POU4F2
Entrez Gene ID	5458
Full Name	POU class 4 homeobox 2
Synonyms	BRN3.2,BRN3B,Brn-3b
General protein information	Preferred Names POU class 4 homeobox 2 Names POU domain, class 4, transcription factor 2 Brn3b POU domain transcription factor POU domain protein brain-3B brain-specific homeobox/POU domain protein 3B
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q31.22
Summary	The protein encoded by this gene is a member of the POU-domain transcription factor family and may be involved in maintaining visual system neurons in the retina. The level of the encoded protein is also elevated in a majority of breast cancers, resulting in accelerated tumor growth. [provided by RefSeq, Sep 2011].
Disorder MIM:	113725

mRNA and Protein(s)

mRNA	Protein	Name
NM_004575.3	NP_004566.2	POU domain, class 4, transcription factor 2
NM_004575.2	NP_004566.2	POU domain, class 4, transcription factor 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pou4f2	NP_620394.2
Danio rerio (zebrafish)	pou4f2	NP_997972.1
Homo sapiens (human)	POU4F2	NP_004566.2
Bos taurus (cattle)	POU4F2	NP_001179881.1
Canis lupus familiaris (dog)	POU4F2	XP_853453.1
Xenopus tropicalis (tropical clawed frog)	pou4f2	XP_004911191.1
Pan troglodytes (chimpanzee)	POU4F2	XP_526699.2
	POU4F2	XP_001098227.1
Rattus norvegicus (Norway rat)	Pou4f2	NP_599182.1

Gene Ontology
Bibliography

Related articles in PubMed

An epigenetic biomarker combination of PCDH17 and POU4F2 detects bladder cancer accurately by methylation analyses of urine sediment DNA in Han Chinese.
Wang Y, Yu Y, Ye R, Zhang D, Li Q, An D, Fang L, Lin Y, Hou Y, Xu A, Fu Y, Lu W, Chen X, Chen M, Zhang M, Jiang H, Zhang C, Dong P, Li C, Chen J, Yang G, Liu C, Cai Z, Zhou F, Wu S
Oncotarget7(3)2754-64(2016 Jan)

The transcriptional regulator BCL6 participates in the secondary gene regulatory response to vitamin D.
Nurminen V, Neme A, Ryyn?nen J, Heikkinen S, Seuter S, Carlberg C
Biochimica et biophysica acta1849(3)300-8(2015 Mar)

RIT2, a neuron-specific small guanosine triphosphatase, is expressed in retinal neuronal cells and its promoter is modulated by the POU4 transcription factors.
Zhang L, Wahlin K, Li Y, Masuda T, Yang Z, Zack DJ, Esumi N
Molecular vision191371-86(2013)

Diagnosis of bladder cancer recurrence based on urinary levels of EOMES, HOXA9, POU4F2, TWIST1, VIM, and ZNF154 hypermethylation.
Reinert T, Borre M, Christiansen A, Hermann GG, ?rntoft TF, Dyrskj?t L
PloS one7(10)e46297(2012)

Hsp-27 induction requires POU4F2/Brn-3b TF in doxorubicin-treated breast cancer cells, whereas phosphorylation alters its cellular localisation following drug treatment.
Fujita R, Ounzain S, Wang AC, Heads RJ, Budhram-Mahadeo VS
Cell stress & chaperones16(4)427-39(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DNA methylation in a combination of POU4F2/PCDH17 has yielded the highest sensitivity and specificity of 90.00% and 93.96% in all the 312 individuals, showing the capability of detecting bladder cancer effectively among pathologically varied sample groups.
Title: An epigenetic biomarker combination of PCDH17 and POU4F2 detects bladder cancer accurately by methylation analyses of urine sediment DNA in Han Chinese.

The genes BCL6, NFE2, POU4F2 and ELF4 are primary 1,25(OH)2D3 targets in THP-1 cells
Title: The transcriptional regulator BCL6 participates in the secondary gene regulatory response to vitamin D.

Methylation levels of EOMES, HOXA9, POU4F2, TWIST1, VIM, and ZNF154 in urine specimens are promising diagnostic biomarkers for bladder cancer recurrence surveillance
Title: Diagnosis of bladder cancer recurrence based on urinary levels of EOMES, HOXA9, POU4F2, TWIST1, VIM, and ZNF154 hypermethylation.

Brn3b specifies the RGC fate from multipotential precursors not only by promoting RGC differentiation but also by suppressing non-RGC differentiation programs as a safeguard mechanism.
Title: A comprehensive negative regulatory program controlled by Brn3b to ensure ganglion cell specification from multipotential retinal precursors.

May act to alter growth properties of breast cancer and neuroblastoma cells by enhancing cyclin D1 expression in these tumor cells.
Title: Proliferation-associated Brn-3b transcription factor can activate cyclin D1 expression in neuroblastoma and breast cancer cells.

The following POU4F2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POU4F2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04465	NM_004575.3 Latest version!	Homo sapiens POU class 4 homeobox 2 (POU4F2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$265.30 $379.00
View Old Accession Versions >>
OHu04465	NM_004575.2	Homo sapiens POU class 4 homeobox 2 (POU4F2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$265.30 $379.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POU4F2 ( NM_004575.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004566.2 Homo sapiens POU class 4 homeobox 2 (POU4F2), mRNA.

Price & Availability↑

CloneID	OHu04465
Clone ID Related Accession (Same CDS sequence)	NM_004575.3 , NM_004575.2
Accession Version	NM_004575.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	POU domain, class 4, transcription factor 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB082745.1, BY796838.2, U06233.1, X71488.1 and AC093887.3. On Nov 22, 2018 this sequence version replaced NM_004575.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU439706.1, U06233.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000281321.3/ ENSP00000281321.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATGATGA TGTCCCTGAA CAGCAAGCAG GCGTTTAGCA TGCCGCACGG CGGCAGCCTG 
CACGTGGAGC CCAAGTACTC GGCACTGCAC AGCACCTCGC CGGGCTCCTC GGCTCCCATC 
GCGCCCTCGG CCAGCTCCCC CAGCAGCTCG AGCAACGCTG GTGGTGGCGG CGGCGGCGGC 
GGCGGCGGCG GCGGCGGCGG AGGCCGAAGC AGCAGCTCCA GCAGCAGTGG CAGCAGCGGC 
GGCGGGGGCT CGGAGGCTAT GCGGAGAGCC TGTCTTCCAA CCCCACCGAG CAATATATTC 
GGCGGGCTGG ATGAGAGTCT GCTGGCCCGC GCCGAGGCTC TGGCAGCCGT GGACATCGTC 
TCCCAGAGCA AGAGCCACCA CCACCATCCA CCCCACCACA GCCCCTTCAA ACCGGACGCC 
ACCTACCACA CTATGAATAC CATCCCGTGC ACGTCGGCCG CCTCTTCTTC ATCGGTGCCC 
ATCTCGCACC CTTCCGCGTT GGCGGGCACG CACCACCACC ACCACCATCA CCACCACCAC 
CACCACCAAC CGCACCAGGC GCTGGAGGGC GAGCTGCTGG AGCACCTGAG TCCCGGGCTG 
GCCCTGGGCG CTATGGCGGG CCCCGACGGC GCTGTGGTGT CCACGCCGGC TCACGCGCCG 
CACATGGCCA CCATGAACCC CATGCACCAA GCAGCGCTCA GCATGGCCCA CGCGCACGGG 
CTGCCGTCGC ACATGGGCTG CATGAGCGAC GTGGACGCCG ACCCGCGGGA CCTGGAGGCA 
TTCGCCGAGC GCTTCAAGCA GCGACGCATC AAGCTGGGGG TGACCCAGGC AGATGTGGGC 
TCCGCGCTGG CCAACCTCAA GATCCCCGGC GTGGGCTCGC TTAGCCAGAG CACCATCTGC 
AGGTTCGAGT CCCTCACACT GTCCCACAAT AATATGATCG CGCTCAAACC CATCCTGCAG 
GCATGGCTCG AGGAGGCCGA GAAGTCCCAC CGCGAGAAGC TCACCAAGCC TGAACTCTTC 
AATGGCGCGG AGAAGAAGCG CAAGCGCACG TCCATCGCTG CGCCAGAGAA GCGCTCGCTC 
GAAGCCTACT TTGCCATTCA GCCTCGGCCC TCCTCTGAAA AGATCGCCGC CATCGCGGAG 
AAGCTGGACC TGAAGAAAAA CGTGGTGCGC GTCTGGTTCT GCAACCAGAG GCAGAAACAG 
AAAAGAATGA AATATTCCGC CGGCATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004566.2
CDS	249..1478
Translation	MMMMSLNSKQAFSMPHGGSLHVEPKYSALHSTSPGSSAPIAPSASSPSSSSNAGGGGGGGGGGGGGGGRSSSSSSSGSSGGGGSEAMRRACLPTPPSNIFGGLDESLLARAEALAAVDIVSQSKSHHHHPPHHSPFKPDATYHTMNTIPCTSAASSSSVPISHPSALAGTHHHHHHHHHHHHQPHQALEGELLEHLSPGLALGAMAGPDGAVVSTPAHAPHMATMNPMHQAALSMAHAHGLPSHMGCMSDVDADPRDLEAFAERFKQRRIKLGVTQADVGSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEKSHREKLTKPELFNGAEKKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSAGI

Target ORF information:

RefSeq Version	NM_004575.3
Organism	Homo sapiens(human)
Definition	Homo sapiens POU class 4 homeobox 2 (POU4F2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004575.3

ORF Insert Sequence:

ATGATGATGA TGTCCCTGAA CAGCAAGCAG GCGTTTAGCA TGCCGCACGG CGGCAGCCTG 
CACGTGGAGC CCAAGTACTC GGCACTGCAC AGCACCTCGC CGGGCTCCTC GGCTCCCATC 
GCGCCCTCGG CCAGCTCCCC CAGCAGCTCG AGCAACGCTG GTGGTGGCGG CGGCGGCGGC 
GGCGGCGGCG GCGGCGGCGG AGGCCGAAGC AGCAGCTCCA GCAGCAGTGG CAGCAGCGGC 
GGCGGGGGCT CGGAGGCTAT GCGGAGAGCC TGTCTTCCAA CCCCACCGAG CAATATATTC 
GGCGGGCTGG ATGAGAGTCT GCTGGCCCGC GCCGAGGCTC TGGCAGCCGT GGACATCGTC 
TCCCAGAGCA AGAGCCACCA CCACCATCCA CCCCACCACA GCCCCTTCAA ACCGGACGCC 
ACCTACCACA CTATGAATAC CATCCCGTGC ACGTCGGCCG CCTCTTCTTC ATCGGTGCCC 
ATCTCGCACC CTTCCGCGTT GGCGGGCACG CACCACCACC ACCACCATCA CCACCACCAC 
CACCACCAAC CGCACCAGGC GCTGGAGGGC GAGCTGCTGG AGCACCTGAG TCCCGGGCTG 
GCCCTGGGCG CTATGGCGGG CCCCGACGGC GCTGTGGTGT CCACGCCGGC TCACGCGCCG 
CACATGGCCA CCATGAACCC CATGCACCAA GCAGCGCTCA GCATGGCCCA CGCGCACGGG 
CTGCCGTCGC ACATGGGCTG CATGAGCGAC GTGGACGCCG ACCCGCGGGA CCTGGAGGCA 
TTCGCCGAGC GCTTCAAGCA GCGACGCATC AAGCTGGGGG TGACCCAGGC AGATGTGGGC 
TCCGCGCTGG CCAACCTCAA GATCCCCGGC GTGGGCTCGC TTAGCCAGAG CACCATCTGC 
AGGTTCGAGT CCCTCACACT GTCCCACAAT AATATGATCG CGCTCAAACC CATCCTGCAG 
GCATGGCTCG AGGAGGCCGA GAAGTCCCAC CGCGAGAAGC TCACCAAGCC TGAACTCTTC 
AATGGCGCGG AGAAGAAGCG CAAGCGCACG TCCATCGCTG CGCCAGAGAA GCGCTCGCTC 
GAAGCCTACT TTGCCATTCA GCCTCGGCCC TCCTCTGAAA AGATCGCCGC CATCGCGGAG 
AAGCTGGACC TGAAGAAAAA CGTGGTGCGC GTCTGGTTCT GCAACCAGAG GCAGAAACAG 
AAAAGAATGA AATATTCCGC CGGCATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POU4F2 ( NM_004575.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_004566.2 Homo sapiens POU class 4 homeobox 2 (POU4F2), mRNA.

Price & Availability↑

CloneID	OHu04465
Clone ID Related Accession (Same CDS sequence)	NM_004575.3 , NM_004575.2
Accession Version	NM_004575.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	POU domain, class 4, transcription factor 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB082745.1, BY796838.2, U06233.1 and X71488.1. On Jul 14, 2006 this sequence version replaced NM_004575.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U06233.1, EU439706.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGATGA TGTCCCTGAA CAGCAAGCAG GCGTTTAGCA TGCCGCACGG CGGCAGCCTG 
CACGTGGAGC CCAAGTACTC GGCACTGCAC AGCACCTCGC CGGGCTCCTC GGCTCCCATC 
GCGCCCTCGG CCAGCTCCCC CAGCAGCTCG AGCAACGCTG GTGGTGGCGG CGGCGGCGGC 
GGCGGCGGCG GCGGCGGCGG AGGCCGAAGC AGCAGCTCCA GCAGCAGTGG CAGCAGCGGC 
GGCGGGGGCT CGGAGGCTAT GCGGAGAGCC TGTCTTCCAA CCCCACCGAG CAATATATTC 
GGCGGGCTGG ATGAGAGTCT GCTGGCCCGC GCCGAGGCTC TGGCAGCCGT GGACATCGTC 
TCCCAGAGCA AGAGCCACCA CCACCATCCA CCCCACCACA GCCCCTTCAA ACCGGACGCC 
ACCTACCACA CTATGAATAC CATCCCGTGC ACGTCGGCCG CCTCTTCTTC ATCGGTGCCC 
ATCTCGCACC CTTCCGCGTT GGCGGGCACG CACCACCACC ACCACCATCA CCACCACCAC 
CACCACCAAC CGCACCAGGC GCTGGAGGGC GAGCTGCTGG AGCACCTGAG TCCCGGGCTG 
GCCCTGGGCG CTATGGCGGG CCCCGACGGC GCTGTGGTGT CCACGCCGGC TCACGCGCCG 
CACATGGCCA CCATGAACCC CATGCACCAA GCAGCGCTCA GCATGGCCCA CGCGCACGGG 
CTGCCGTCGC ACATGGGCTG CATGAGCGAC GTGGACGCCG ACCCGCGGGA CCTGGAGGCA 
TTCGCCGAGC GCTTCAAGCA GCGACGCATC AAGCTGGGGG TGACCCAGGC AGATGTGGGC 
TCCGCGCTGG CCAACCTCAA GATCCCCGGC GTGGGCTCGC TTAGCCAGAG CACCATCTGC 
AGGTTCGAGT CCCTCACACT GTCCCACAAT AATATGATCG CGCTCAAACC CATCCTGCAG 
GCATGGCTCG AGGAGGCCGA GAAGTCCCAC CGCGAGAAGC TCACCAAGCC TGAACTCTTC 
AATGGCGCGG AGAAGAAGCG CAAGCGCACG TCCATCGCTG CGCCAGAGAA GCGCTCGCTC 
GAAGCCTACT TTGCCATTCA GCCTCGGCCC TCCTCTGAAA AGATCGCCGC CATCGCGGAG 
AAGCTGGACC TGAAGAAAAA CGTGGTGCGC GTCTGGTTCT GCAACCAGAG GCAGAAACAG 
AAAAGAATGA AATATTCCGC CGGCATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004566.2
CDS	249..1478
Translation	MMMMSLNSKQAFSMPHGGSLHVEPKYSALHSTSPGSSAPIAPSASSPSSSSNAGGGGGGGGGGGGGGGRSSSSSSSGSSGGGGSEAMRRACLPTPPSNIFGGLDESLLARAEALAAVDIVSQSKSHHHHPPHHSPFKPDATYHTMNTIPCTSAASSSSVPISHPSALAGTHHHHHHHHHHHHQPHQALEGELLEHLSPGLALGAMAGPDGAVVSTPAHAPHMATMNPMHQAALSMAHAHGLPSHMGCMSDVDADPRDLEAFAERFKQRRIKLGVTQADVGSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEKSHREKLTKPELFNGAEKKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSAGI

Target ORF information:

RefSeq Version	NM_004575.2
Organism	Homo sapiens(human)
Definition	Homo sapiens POU class 4 homeobox 2 (POU4F2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004575.2

ORF Insert Sequence:

ATGATGATGA TGTCCCTGAA CAGCAAGCAG GCGTTTAGCA TGCCGCACGG CGGCAGCCTG 
CACGTGGAGC CCAAGTACTC GGCACTGCAC AGCACCTCGC CGGGCTCCTC GGCTCCCATC 
GCGCCCTCGG CCAGCTCCCC CAGCAGCTCG AGCAACGCTG GTGGTGGCGG CGGCGGCGGC 
GGCGGCGGCG GCGGCGGCGG AGGCCGAAGC AGCAGCTCCA GCAGCAGTGG CAGCAGCGGC 
GGCGGGGGCT CGGAGGCTAT GCGGAGAGCC TGTCTTCCAA CCCCACCGAG CAATATATTC 
GGCGGGCTGG ATGAGAGTCT GCTGGCCCGC GCCGAGGCTC TGGCAGCCGT GGACATCGTC 
TCCCAGAGCA AGAGCCACCA CCACCATCCA CCCCACCACA GCCCCTTCAA ACCGGACGCC 
ACCTACCACA CTATGAATAC CATCCCGTGC ACGTCGGCCG CCTCTTCTTC ATCGGTGCCC 
ATCTCGCACC CTTCCGCGTT GGCGGGCACG CACCACCACC ACCACCATCA CCACCACCAC 
CACCACCAAC CGCACCAGGC GCTGGAGGGC GAGCTGCTGG AGCACCTGAG TCCCGGGCTG 
GCCCTGGGCG CTATGGCGGG CCCCGACGGC GCTGTGGTGT CCACGCCGGC TCACGCGCCG 
CACATGGCCA CCATGAACCC CATGCACCAA GCAGCGCTCA GCATGGCCCA CGCGCACGGG 
CTGCCGTCGC ACATGGGCTG CATGAGCGAC GTGGACGCCG ACCCGCGGGA CCTGGAGGCA 
TTCGCCGAGC GCTTCAAGCA GCGACGCATC AAGCTGGGGG TGACCCAGGC AGATGTGGGC 
TCCGCGCTGG CCAACCTCAA GATCCCCGGC GTGGGCTCGC TTAGCCAGAG CACCATCTGC 
AGGTTCGAGT CCCTCACACT GTCCCACAAT AATATGATCG CGCTCAAACC CATCCTGCAG 
GCATGGCTCG AGGAGGCCGA GAAGTCCCAC CGCGAGAAGC TCACCAAGCC TGAACTCTTC 
AATGGCGCGG AGAAGAAGCG CAAGCGCACG TCCATCGCTG CGCCAGAGAA GCGCTCGCTC 
GAAGCCTACT TTGCCATTCA GCCTCGGCCC TCCTCTGAAA AGATCGCCGC CATCGCGGAG 
AAGCTGGACC TGAAGAAAAA CGTGGTGCGC GTCTGGTTCT GCAACCAGAG GCAGAAACAG 
AAAAGAATGA AATATTCCGC CGGCATTTAG