RNF186 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RNF186
Entrez Gene ID	54546
Full Name	ring finger protein 186
General protein information	Preferred Names ring finger protein 186 Names RING finger protein 186
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.13
Disorder MIM:	617163

mRNA and Protein(s)

mRNA	Protein	Name
NM_019062.2	NP_061935.1	E3 ubiquitin-protein ligase RNF186
NM_019062.1	NP_061935.1	RING finger protein 186

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rnf186	NP_080062.4
Rattus norvegicus (Norway rat)	Rnf186	NP_001103062.1
Homo sapiens (human)	RNF186	NP_061935.1
	RNF186	XP_001094241.1
Canis lupus familiaris (dog)	RNF186	XP_544526.1
Bos taurus (cattle)	RNF186	NP_001030388.1
Gallus gallus (chicken)	RNF186	XP_004947539.1
Pan troglodytes (chimpanzee)	RNF186	XP_001160206.1
Xenopus tropicalis (tropical clawed frog)	rnf186	XP_004916480.1

Gene Ontology
Bibliography

Related articles in PubMed

A protein-truncating R179X variant in RNF186 confers protection against ulcerative colitis.
Rivas MA, Graham D, Sulem P, Stevens C, Desch AN, Goyette P, Gudbjartsson D, Jonsdottir I, Thorsteinsdottir U, Degenhardt F, Mucha S, Kurki MI, Li D, D'Amato M, Annese V, Vermeire S, Weersma RK, Halfvarson J, Paavola-Sakki P, Lappalainen M, Lek M, Cummings B, Tukiainen T, Haritunians T, Halme L, Koskinen LL, Ananthakrishnan AN, Luo Y, Heap GA, Visschedijk MC, , , MacArthur DG, Neale BM, Ahmad T, Anderson CA, Brant SR, Duerr RH, Silverberg MS, Cho JH, Palotie A, Saavalainen P, Kontula K, F?rkkil? M, McGovern DP, Franke A, Stefansson K, Rioux JD, Xavier RJ, Daly MJ, Barrett J, de Lane K, Edwards C, Hart A, Hawkey C, Jostins L, Kennedy N, Lamb C, Lee J, Lees C, Mansfield J, Mathew C, Mowatt C, Newman B, Nimmo E, Parkes M, Pollard M, Prescott N, Randall J, Rice D, Satsangi J, Simmons A, Tremelling M, Uhlig H, Wilson D, Abraham C, Achkar JP, Bitton A, Boucher G, Croitoru K, Fleshner P, Glas J, Kugathasan S, Limbergen JV, Milgrom R, Proctor D, Regueiro M, Schumm PL, Sharma Y, Stempak JM, Targan SR, Wang MH
Nature communications712342(2016 Aug)

A novel RING finger E3 ligase RNF186 regulate ER stress-mediated apoptosis through interaction with BNip1.
Wang P, Wu Y, Li Y, Zheng J, Tang J
Cellular signalling25(11)2320-33(2013 Nov)

Genome-wide association study of ulcerative colitis in Koreans suggests extensive overlapping of genetic susceptibility with Caucasians.
Yang SK, Hong M, Zhao W, Jung Y, Tayebi N, Ye BD, Kim KJ, Park SH, Lee I, Shin HD, Cheong HS, Kim LH, Kim HJ, Jung SA, Kang D, Youn HS, Liu J, Song K
Inflammatory bowel diseases19(5)954-66(2013 Apr)

Deep resequencing of GWAS loci identifies rare variants in CARD9, IL23R and RNF186 that are associated with ulcerative colitis.
Beaudoin M, Goyette P, Boucher G, Lo KS, Rivas MA, Stevens C, Alikashani A, Ladouceur M, Ellinghaus D, T?rkvist L, Goel G, Lagac? C, Annese V, Bitton A, Begun J, Brant SR, Bresso F, Cho JH, Duerr RH, Halfvarson J, McGovern DP, Radford-Smith G, Schreiber S, Schumm PL, Sharma Y, Silverberg MS, Weersma RK, , , , D'Amato M, Vermeire S, Franke A, Lettre G, Xavier RJ, Daly MJ, Rioux JD
PLoS genetics9(9)e1003723(2013)

Genome-wide association identifies multiple ulcerative colitis susceptibility loci.
McGovern DP, Gardet A, T?rkvist L, Goyette P, Essers J, Taylor KD, Neale BM, Ong RT, Lagac? C, Li C, Green T, Stevens CR, Beauchamp C, Fleshner PR, Carlson M, D'Amato M, Halfvarson J, Hibberd ML, L?rdal M, Padyukov L, Andriulli A, Colombo E, Latiano A, Palmieri O, Bernard EJ, Deslandres C, Hommes DW, de Jong DJ, Stokkers PC, Weersma RK, , Sharma Y, Silverberg MS, Cho JH, Wu J, Roeder K, Brant SR, Schumm LP, Duerr RH, Dubinsky MC, Glazer NL, Haritunians T, Ippoliti A, Melmed GY, Siscovick DS, Vasiliauskas EA, Targan SR, Annese V, Wijmenga C, Pettersson S, Rotter JI, Xavier RJ, Daly MJ, Rioux JD, Seielstad M
Nature genetics42(4)332-7(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

BNip1 functions as a downstream modulator of RNF186 to direct endoplasmic reticulum stress-associated apoptotic signaling.
Title: A novel RING finger E3 ligase RNF186 regulate ER stress-mediated apoptosis through interaction with BNip1.

Single-nucleotide polymorphism in RNF186 gene is associated with ulcerative colitis.
Title: Genome-wide association study of ulcerative colitis in Koreans suggests extensive overlapping of genetic susceptibility with Caucasians.

Our results confirmed significant association to rare non-synonymous coding variants in both IL23R and CARD9, previously identified from sequencing of CD loci, as well as identified a novel association in RNF186
Title: Deep resequencing of GWAS loci identifies rare variants in CARD9, IL23R and RNF186 that are associated with ulcerative colitis.

The following RNF186 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNF186 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13404	NM_019062.2 Latest version!	Homo sapiens ring finger protein 186 (RNF186), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu13404	NM_019062.1	Homo sapiens ring finger protein 186 (RNF186), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNF186 ( NM_019062.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_061935.1 Homo sapiens ring finger protein 186 (RNF186), mRNA.

Price & Availability↑

CloneID	OHu13404
Clone ID Related Accession (Same CDS sequence)	NM_019062.2 , NM_019062.1
Accession Version	NM_019062.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF186
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL391883.17 and AK000232.1. On Nov 23, 2018 this sequence version replaced NM_019062.1. ##Evidence-Data-START## Transcript is intronless :: AK000232.1, BC030960.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000375121.4/ ENSP00000364263.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCTGCA CCAAGACCCT GCAACAGTCC CAGCCCATCT CCGCAGGAGC CACCACAACC 
ACCACCGCTG TGGCCCCTGC TGGGGGTCAT TCTGGCTCCA CAGAATGTGA CCTGGAGTGT 
CTGGTGTGCC GGGAGCCCTA CAGCTGTCCC CGGTTGCCCA AGCTGCTGGC CTGCCAGCAT 
GCCTTCTGCG CCATCTGCCT GAAGCTCCTG CTGTGCGTGC AGGACAACAC CTGGTCCATC 
ACCTGCCCGC TGTGCCGCAA GGTCACCGCC GTCCCCGGGG GCCTCATCTG CAGCCTGCGC 
GACCATGAGG CGGTGGTGGG GCAGCTGGCC CAGCCATGCA CAGAGGTATC GCTCTGTCCT 
CAGGGGCTGG TGGATCCTGC TGACTTGGCA GCAGGACACC CCAGCTTGGT GGGAGAGGAT 
GGACAGGATG AAGTAAGTGC AAACCACGTG GCAGCCCGGC GCCTGGCCGC GCACCTACTC 
CTGCTGGCCT TGCTCATTAT CCTCATCGGG CCCTTCATCT ACCCGGGTGT CTTACGATGG 
GTGCTCACCT TCATCATCGC CCTGGCCCTG CTGATGTCCA CCCTCTTCTG CTGTCTCCCC 
AGCACCCGGG GCAGCTGCTG GCCCTCCTCC AGGACTCTCT TCTGCAGAGA GCAGAAACAC 
AGCCACATCT CTTCCATTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061935.1
CDS	183..866
Translation	MACTKTLQQSQPISAGATTTTTAVAPAGGHSGSTECDLECLVCREPYSCPRLPKLLACQHAFCAICLKLLLCVQDNTWSITCPLCRKVTAVPGGLICSLRDHEAVVGQLAQPCTEVSLCPQGLVDPADLAAGHPSLVGEDGQDEVSANHVAARRLAAHLLLLALLIILIGPFIYPGVLRWVLTFIIALALLMSTLFCCLPSTRGSCWPSSRTLFCREQKHSHISSIA

Target ORF information:

RefSeq Version	NM_019062.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 186 (RNF186), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019062.2

ORF Insert Sequence:

ATGGCCTGCA CCAAGACCCT GCAACAGTCC CAGCCCATCT CCGCAGGAGC CACCACAACC 
ACCACCGCTG TGGCCCCTGC TGGGGGTCAT TCTGGCTCCA CAGAATGTGA CCTGGAGTGT 
CTGGTGTGCC GGGAGCCCTA CAGCTGTCCC CGGTTGCCCA AGCTGCTGGC CTGCCAGCAT 
GCCTTCTGCG CCATCTGCCT GAAGCTCCTG CTGTGCGTGC AGGACAACAC CTGGTCCATC 
ACCTGCCCGC TGTGCCGCAA GGTCACCGCC GTCCCCGGGG GCCTCATCTG CAGCCTGCGC 
GACCATGAGG CGGTGGTGGG GCAGCTGGCC CAGCCATGCA CAGAGGTATC GCTCTGTCCT 
CAGGGGCTGG TGGATCCTGC TGACTTGGCA GCAGGACACC CCAGCTTGGT GGGAGAGGAT 
GGACAGGATG AAGTAAGTGC AAACCACGTG GCAGCCCGGC GCCTGGCCGC GCACCTACTC 
CTGCTGGCCT TGCTCATTAT CCTCATCGGG CCCTTCATCT ACCCGGGTGT CTTACGATGG 
GTGCTCACCT TCATCATCGC CCTGGCCCTG CTGATGTCCA CCCTCTTCTG CTGTCTCCCC 
AGCACCCGGG GCAGCTGCTG GCCCTCCTCC AGGACTCTCT TCTGCAGAGA GCAGAAACAC 
AGCCACATCT CTTCCATTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF186 ( NM_019062.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_061935.1 Homo sapiens ring finger protein 186 (RNF186), mRNA.

Price & Availability↑

CloneID	OHu13404
Clone ID Related Accession (Same CDS sequence)	NM_019062.2 , NM_019062.1
Accession Version	NM_019062.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	RING finger protein 186
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK000232.1. ##Evidence-Data-START## Transcript is intronless :: AK000232.1, AK222991.1 [ECO:0000345] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTGCA CCAAGACCCT GCAACAGTCC CAGCCCATCT CCGCAGGAGC CACCACAACC 
ACCACCGCTG TGGCCCCTGC TGGGGGTCAT TCTGGCTCCA CAGAATGTGA CCTGGAGTGT 
CTGGTGTGCC GGGAGCCCTA CAGCTGTCCC CGGTTGCCCA AGCTGCTGGC CTGCCAGCAT 
GCCTTCTGCG CCATCTGCCT GAAGCTCCTG CTGTGCGTGC AGGACAACAC CTGGTCCATC 
ACCTGCCCGC TGTGCCGCAA GGTCACCGCC GTCCCCGGGG GCCTCATCTG CAGCCTGCGC 
GACCATGAGG CGGTGGTGGG GCAGCTGGCC CAGCCATGCA CAGAGGTATC GCTCTGTCCT 
CAGGGGCTGG TGGATCCTGC TGACTTGGCA GCAGGACACC CCAGCTTGGT GGGAGAGGAT 
GGACAGGATG AAGTAAGTGC AAACCACGTG GCAGCCCGGC GCCTGGCCGC GCACCTACTC 
CTGCTGGCCT TGCTCATTAT CCTCATCGGG CCCTTCATCT ACCCGGGTGT CTTACGATGG 
GTGCTCACCT TCATCATCGC CCTGGCCCTG CTGATGTCCA CCCTCTTCTG CTGTCTCCCC 
AGCACCCGGG GCAGCTGCTG GCCCTCCTCC AGGACTCTCT TCTGCAGAGA GCAGAAACAC 
AGCCACATCT CTTCCATTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061935.1
CDS	178..861
Translation	MACTKTLQQSQPISAGATTTTTAVAPAGGHSGSTECDLECLVCREPYSCPRLPKLLACQHAFCAICLKLLLCVQDNTWSITCPLCRKVTAVPGGLICSLRDHEAVVGQLAQPCTEVSLCPQGLVDPADLAAGHPSLVGEDGQDEVSANHVAARRLAAHLLLLALLIILIGPFIYPGVLRWVLTFIIALALLMSTLFCCLPSTRGSCWPSSRTLFCREQKHSHISSIA

Target ORF information:

RefSeq Version	NM_019062.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 186 (RNF186), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019062.1

ORF Insert Sequence:

ATGGCCTGCA CCAAGACCCT GCAACAGTCC CAGCCCATCT CCGCAGGAGC CACCACAACC 
ACCACCGCTG TGGCCCCTGC TGGGGGTCAT TCTGGCTCCA CAGAATGTGA CCTGGAGTGT 
CTGGTGTGCC GGGAGCCCTA CAGCTGTCCC CGGTTGCCCA AGCTGCTGGC CTGCCAGCAT 
GCCTTCTGCG CCATCTGCCT GAAGCTCCTG CTGTGCGTGC AGGACAACAC CTGGTCCATC 
ACCTGCCCGC TGTGCCGCAA GGTCACCGCC GTCCCCGGGG GCCTCATCTG CAGCCTGCGC 
GACCATGAGG CGGTGGTGGG GCAGCTGGCC CAGCCATGCA CAGAGGTATC GCTCTGTCCT 
CAGGGGCTGG TGGATCCTGC TGACTTGGCA GCAGGACACC CCAGCTTGGT GGGAGAGGAT 
GGACAGGATG AAGTAAGTGC AAACCACGTG GCAGCCCGGC GCCTGGCCGC GCACCTACTC 
CTGCTGGCCT TGCTCATTAT CCTCATCGGG CCCTTCATCT ACCCGGGTGT CTTACGATGG 
GTGCTCACCT TCATCATCGC CCTGGCCCTG CTGATGTCCA CCCTCTTCTG CTGTCTCCCC 
AGCACCCGGG GCAGCTGCTG GCCCTCCTCC AGGACTCTCT TCTGCAGAGA GCAGAAACAC 
AGCCACATCT CTTCCATTGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A protein-truncating R179X variant in RNF186 confers protection against ulcerative colitis.
Nature communications712342(2016 Aug)
Rivas MA,Graham D,Sulem P,Stevens C,Desch AN,Goyette P,Gudbjartsson D,Jonsdottir I,Thorsteinsdottir U,Degenhardt F,Mucha S,Kurki MI,Li D,D'Amato M,Annese V,Vermeire S,Weersma RK,Halfvarson J,Paavola-Sakki P,Lappalainen M,Lek M,Cummings B,Tukiainen T,Haritunians T,Halme L,Koskinen LL,Ananthakrishnan AN,Luo Y,Heap GA,Visschedijk MC,,,MacArthur DG,Neale BM,Ahmad T,Anderson CA,Brant SR,Duerr RH,Silverberg MS,Cho JH,Palotie A,Saavalainen P,Kontula K,F?rkkil? M,McGovern DP,Franke A,Stefansson K,Rioux JD,Xavier RJ,Daly MJ,Barrett J,de Lane K,Edwards C,Hart A,Hawkey C,Jostins L,Kennedy N,Lamb C,Lee J,Lees C,Mansfield J,Mathew C,Mowatt C,Newman B,Nimmo E,Parkes M,Pollard M,Prescott N,Randall J,Rice D,Satsangi J,Simmons A,Tremelling M,Uhlig H,Wilson D,Abraham C,Achkar JP,Bitton A,Boucher G,Croitoru K,Fleshner P,Glas J,Kugathasan S,Limbergen JV,Milgrom R,Proctor D,Regueiro M,Schumm PL,Sharma Y,Stempak JM,Targan SR,Wang MH

A novel RING finger E3 ligase RNF186 regulate ER stress-mediated apoptosis through interaction with BNip1.
Cellular signalling25(11)2320-33(2013 Nov)
Wang P,Wu Y,Li Y,Zheng J,Tang J

Genome-wide association study of ulcerative colitis in Koreans suggests extensive overlapping of genetic susceptibility with Caucasians.
Inflammatory bowel diseases19(5)954-66(2013 Apr)
Yang SK,Hong M,Zhao W,Jung Y,Tayebi N,Ye BD,Kim KJ,Park SH,Lee I,Shin HD,Cheong HS,Kim LH,Kim HJ,Jung SA,Kang D,Youn HS,Liu J,Song K

Deep resequencing of GWAS loci identifies rare variants in CARD9, IL23R and RNF186 that are associated with ulcerative colitis.
PLoS genetics9(9)e1003723(2013)
Beaudoin M,Goyette P,Boucher G,Lo KS,Rivas MA,Stevens C,Alikashani A,Ladouceur M,Ellinghaus D,T?rkvist L,Goel G,Lagac? C,Annese V,Bitton A,Begun J,Brant SR,Bresso F,Cho JH,Duerr RH,Halfvarson J,McGovern DP,Radford-Smith G,Schreiber S,Schumm PL,Sharma Y,Silverberg MS,Weersma RK,,,,D'Amato M,Vermeire S,Franke A,Lettre G,Xavier RJ,Daly MJ,Rioux JD

Genome-wide association identifies multiple ulcerative colitis susceptibility loci.
Nature genetics42(4)332-7(2010 Apr)
McGovern DP,Gardet A,T?rkvist L,Goyette P,Essers J,Taylor KD,Neale BM,Ong RT,Lagac? C,Li C,Green T,Stevens CR,Beauchamp C,Fleshner PR,Carlson M,D'Amato M,Halfvarson J,Hibberd ML,L?rdal M,Padyukov L,Andriulli A,Colombo E,Latiano A,Palmieri O,Bernard EJ,Deslandres C,Hommes DW,de Jong DJ,Stokkers PC,Weersma RK,,Sharma Y,Silverberg MS,Cho JH,Wu J,Roeder K,Brant SR,Schumm LP,Duerr RH,Dubinsky MC,Glazer NL,Haritunians T,Ippoliti A,Melmed GY,Siscovick DS,Vasiliauskas EA,Targan SR,Annese V,Wijmenga C,Pettersson S,Rotter JI,Xavier RJ,Daly MJ,Rioux JD,Seielstad M

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RNF186 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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