USP53 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	USP53
Entrez Gene ID	54532
Full Name	ubiquitin specific peptidase 53
General protein information	Preferred Names ubiquitin specific peptidase 53 Names inactive ubiquitin carboxyl-terminal hydrolase 53 inactive ubiquitin-specific peptidase 53 ubiquitin specific protease 53 ubiquitin specific proteinase 53
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q26
Disorder MIM:	617431

mRNA and Protein(s)

mRNA	Protein	Name
XM_017008312.2	XP_016863801.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
XM_005263073.4	XP_005263130.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
XM_017008318.2	XP_016863807.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X5
XM_005263077.3	XP_005263134.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X5
XM_017008318.1	XP_016863807.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X5
XM_005263073.3	XP_005263130.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
NM_001371396.1	NP_001358325.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform 2
NM_001371398.1	NP_001358327.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform 3
NM_001371395.1	NP_001358324.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform 1
XM_017008313.1	XP_016863802.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
XM_005263075.3	XP_005263132.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X2
XM_006714240.1	XP_006714303.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X4
XM_017008317.1	XP_016863806.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X5
XM_005263076.2	XP_005263133.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X3
XM_017008316.1	XP_016863805.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X3
XM_017008316.2	XP_016863805.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X3
XM_011532037.2	XP_011530339.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
XM_017008314.1	XP_016863803.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
XM_017008312.1	XP_016863801.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
XM_017008315.1	XP_016863804.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
NM_001371399.1	NP_001358328.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform 1
NM_019050.2	NP_061923.2	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform 1
XM_005263076.3	XP_005263133.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X3
XM_005263077.4	XP_005263134.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X5
XM_017008317.2	XP_016863806.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X5
XM_005263075.2	XP_005263132.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X2
NM_001371397.1	NP_001358326.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform 3
XM_017008315.2	XP_016863804.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
XM_011532038.2	XP_011530340.1	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X4

Pathways from BioSystems

Homologies

Homo sapiens (human)	USP53	NP_061923.2
Gallus gallus (chicken)	USP53	XP_420633.4
Canis lupus familiaris (dog)	USP53	XP_545046.3
Bos taurus (cattle)	USP53	XP_003586226.1
Mus musculus (house mouse)	Usp53	NP_598618.1
Xenopus tropicalis (tropical clawed frog)	usp53	XP_002934301.1
Pan troglodytes (chimpanzee)	USP53	XP_517410.2
	USP53	XP_001098826.2

Gene Ontology
Bibliography

Related articles in PubMed

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

Identification of a candidate gene for astigmatism.
Lopes MC, Hysi PG, Verhoeven VJ, Macgregor S, Hewitt AW, Montgomery GW, Cumberland P, Vingerling JR, Young TL, van Duijn CM, Oostra B, Uitterlinden AG, Rahi JS, Mackey DA, Klaver CC, Andrew T, Hammond CJ
Investigative ophthalmology & visual science54(2)1260-7(2013 Feb)

A systematic gene-based screen of chr4q22-q32 identifies association of a novel susceptibility gene, DKK2, with the quantitative trait of alcohol dependence symptom counts.
Kalsi G, Kuo PH, Aliev F, Alexander J, McMichael O, Patterson DG, Walsh D, Zhao Z, Schuckit M, Nurnberger J Jr, Edenberg H, Kramer J, Hesselbrock V, Tischfield JA, Vladimirov V, Prescott CA, Dick DM, Kendler KS, Riley BP
Human molecular genetics19(12)2497-506(2010 Jun)

The lacrimal gland transcriptome is an unusually rich source of rare and poorly characterized gene transcripts.
Ozyildirim AM, Wistow GJ, Gao J, Wang J, Dickinson DP, Frierson HF Jr, Laurie GW
Investigative ophthalmology & visual science46(5)1572-80(2005 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: A systematic gene-based screen of chr4q22-q32 identifies association of a novel susceptibility gene, DKK2, with the quantitative trait of alcohol dependence symptom counts.

The following USP53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu38976	XM_011532038.2 Latest version!	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu38976	XM_006714240.1 Latest version!	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu38977	XM_005263077.4 Latest version!	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu38977	XM_017008317.2 Latest version!	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu38977	XM_017008318.2 Latest version!	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu38977	NM_001371398.1 Latest version!	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu38977	NM_001371397.1 Latest version!	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu02420	NM_001371395.1 Latest version!	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu02420	NM_001371399.1 Latest version!	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu38975	NM_001371396.1 Latest version!	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu02420	NM_019050.2 Latest version!	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu02420	XM_017008315.2 Latest version!	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu02420	XM_017008314.1 Latest version!	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu02420	XM_017008313.1 Latest version!	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu02420	XM_011532037.2 Latest version!	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu02420	XM_017008312.2 Latest version!	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu02420	XM_005263073.4 Latest version!	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu38974	XM_005263075.3 Latest version!	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu38975	XM_005263076.3 Latest version!	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu38975	XM_017008316.2 Latest version!	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu38977	XM_017008318.1	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu02420	XM_005263073.3	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu38975	XM_017008316.1	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu02420	XM_017008312.1	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu38975	XM_005263076.2	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu38977	XM_005263077.3	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu38977	XM_017008317.1	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu38974	XM_005263075.2	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu02420	XM_017008315.1	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

USP53 ( XM_006714240.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714303.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu38976
Clone ID Related Accession (Same CDS sequence)	XM_006714240.1 , XM_011532038.2
Accession Version	XM_006714240.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATG CTGCGGAGTG CTTTGAAAAT ATGTTGGAGA GGATTCATTT TCACATAGTG 
CCAAGCAGAG ATGCAGACAT GTGTACCTCT AAATCTTGTA TCACTCACCA GAAGTTTGCT 
ATGACTCTGT ATGAACAGTG TGTGTGTCGT AGCTGTGGAG CATCGTCAGA TCCTCTACCT 
TTTACAGAAT TTGTGCGGTA CATTTCTACA ACAGCCTTAT GCAATGAGGT TGAAAGAATG 
TTGGAAAGGC ATGAACGCTT TAAACCTGAA ATGTTTGCAG AATTGCTACA AGCAGCAAAT 
ACAACAGATG ACTATAGGAA ATGTCCTAGT AACTGTGGCC AAAAAATAAA AATTCGCCGT 
GTTTTAATGA ATTGCCCAGA GATTGTTACA ATTGGTTTAG TCTGGGACTC CGAGCATTCT 
GACTTGACCG AAGCTGTTGT TCGGAATCTA GCAACACATC TTTATCTTCC TGGGCTTTTT 
TATAGAGTTA CTGATGAAAA TGCCAAAAAT AGTGAACTTA ACCTTGTTGG TATGATCTGC 
TACACCAGCC AACATTATTG TGCCTTTGCA TTTCACACCA AAAGTTCCAA ATGGGTATTT 
TTTGATGATG CAAATGTGAA AGAGATTGGA ACTAGATGGA AAGATGTTGT CTCCAAATGC 
ATTCGATGCC ACTTTCAGCC ACTACTTTTG TTTTATGCAA ACCCAGATGG CACAGCAGTT 
TCTACTGAGG ATGCACTCAG GCAGGTCATC AGCTGGTCAC ATTACAAATC TGTTGCAGAA 
AATATGGGAT GTGAAAAGCC TGTAATTCAT AAGTCAGATA ATTTAAAAGA AAATGGATTT 
GGTGATCAGG CAAAGCAGAG AGAAAATCAG AAATTTCCAA CTGATAATAT TTCATCATCT 
AATCGGAGCC ACAGTCACAC AGGTGTAGGG AAAGGACCAG CTAAGTTAAG TCACATTGAT 
CAAAGGGAAA AGATAAAAGA CATTTCCAGA GAATGTGCTC TGAAAGCTAT TGAACAGAAA 
AACTTACTTT CTTCACAAAG GAAAGATTTA GAGAAGGGAC AAAGAAAAGA TTTAGGACGA 
CATAGAGATT TGGTTGATGA AGACCTTTCA CATTTCCAAT CTGGATCACC TCCTGCCCCA 
AATGGTTTTA AACAACATGG GAATCCACAT CTATATCATA GTCAAGGAAA AGGATCATAT 
AAACATGACC GAGTTGTACC TCAGAGTCGA GCTTCTGCAC AAATAATAAG TTCAAGTAAA 
TCCCAGATTC TTGCTCCAGG AGAGAAAATA ACTGGCAAAG TTAAGAGTGA CAATGGCACT 
GGATATGACA CAGACAGCAG CCAAGATTCT AGGGATAGAG GAAACAGCTG TGATAGCAGC 
AGTAAAAGCC GGAACCGAGG TTGGAAACCT ATGAGAGAAA CATTAAATGT TGATAGTATT 
TTTAGTGAAA GTGAAAAAAG ACAGCATAGT CCAAGACATA AACCAAATAT CAGTAATAAG 
CCTAAATCTA GCAAGGATCC GAGTTTTAGT AATTGGCCAA AAGAGAATCC AAAGCAAAAA 
GGTTTAATGA CCATATATGA AGATGAAATG AAGCAGGAAA TAGGAAGCAG AAGTTCCCTT 
GAATCTAATG GAAAAGGAGC AGAGAAAAAT AAAGGCCTTG TAGAGGGTAA AGTGCATGGT 
GATAATTGGC AGATGCAAAG GACTGAGTCT GGATATGAAA GCAGTGATCA CATCAGTAAT 
GGTTCTACTA ATTTGGACTC ACCTGTTATC GATGGAAATG GTACAGTAAT GGATATCAGT 
GGTGTTAAAG AAACAGTATG CTTCAGTGAC CAGATTACGA CAAGCAACCT AAATAAAGAA 
CGTGGGGACT GTACCTCCCT TCAGAGCCAA CATCACTTAG AAGGCTTTAG AAAAGAACTC 
AGGAATTTGG AAGCAGGCTA TAAATCTCAT GAATTCCACC CAGAATCACA TTTACAAATA 
AAAAATCATT TGATAAAAAG ATCACATGTA CATGAAGACA ATGGAAAGTT ATTTCCTTCA 
TCCAGTCTAC AAATACCCAA GGACCATAAT GCAAGAGAAC ATATCCACCA GTCAGATGAA 
CAGAAACTTG AAAAACCGAA TGAATGCAAA TTTTCTGAGT GGCTTAATAT AGAAAATTCT 
GAGAGAACAG GTTTGCCTTT TCACGTTGAT AACTCTGCTT CTGGGAAGAG AGTGAACAGT 
AATGAACCAT CTTCATTATG GTCTTCACAC CTAAGAACTG TTGGGTTAAA GCCAGAAACT 
GCTCCTCTCA TCCAGCAACA AAATATCATG GATCAATGTT ACTTTGAGAA CTCTCTATCC 
ACAGAATGTA TAATTCGGTC AGCCAGCAGA TCTGATGGGT GTCAGATGCC AAAACTTTTT 
TGCCAGAATC TACCACCCCC TTTGCCACCA AAGAAATATG CTATAACCAG TGTGCCACAG 
TCAGAGAAAA GCGAATCTAC ACCTGATGTC AAACTTACAG AGGTGTTTAA AGCTACCTCT 
CATCTTCCGA AGCACAGTTT AAGTACAGCT TCAGAACCAA GTTTAGAAGT GAGTACACAT 
ATGAATGATG AAAGACATAA AGAAACATTT CAAGTGAGAG AATGTTTTGG CAACACACCA 
AACTGTCCAT CCAGCTCCTC AACTAACGAT TTTCAGGCAA ACTCAGGTGC CATTGATGCA 
TTTTGCCAAC CAGAACTAGA CTCTATTTCT ACCTGTCCAA ATGAGACAGT TTCATTAACT 
ACCTATTTTT CAGTTGATAG CTGCATGACG GATACATATA GATTGAAATA CCATCAGAGG 
CCCAAGCTCT CTTTTCCAGA GAGCAGTGGC TTTTGTAATA ATTCACTATC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714303.1
CDS	490..3363
Translation	MDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLKENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEGFRKELRNLEAGYKSHEFHPESHLQIKNHLIKRSHVHEDNGKLFPSSSLQIPKDHNAREHIHQSDEQKLEKPNECKFSEWLNIENSERTGLPFHVDNSASGKRVNSNEPSSLWSSHLRTVGLKPETAPLIQQQNIMDQCYFENSLSTECIIRSASRSDGCQMPKLFCQNLPPPLPPKKYAITSVPQSEKSESTPDVKLTEVFKATSHLPKHSLSTASEPSLEVSTHMNDERHKETFQVRECFGNTPNCPSSSSTNDFQANSGAIDAFCQPELDSISTCPNETVSLTTYFSVDSCMTDTYRLKYHQRPKLSFPESSGFCNNSLS

Target ORF information:

RefSeq Version	XM_006714240.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714240.1

ORF Insert Sequence:

ATGGATGATG CTGCGGAGTG CTTTGAAAAT ATGTTGGAGA GGATTCATTT TCACATAGTG 
CCAAGCAGAG ATGCAGACAT GTGTACCTCT AAATCTTGTA TCACTCACCA GAAGTTTGCT 
ATGACTCTGT ATGAACAGTG TGTGTGTCGT AGCTGTGGAG CATCGTCAGA TCCTCTACCT 
TTTACAGAAT TTGTGCGGTA CATTTCTACA ACAGCCTTAT GCAATGAGGT TGAAAGAATG 
TTGGAAAGGC ATGAACGCTT TAAACCTGAA ATGTTTGCAG AATTGCTACA AGCAGCAAAT 
ACAACAGATG ACTATAGGAA ATGTCCTAGT AACTGTGGCC AAAAAATAAA AATTCGCCGT 
GTTTTAATGA ATTGCCCAGA GATTGTTACA ATTGGTTTAG TCTGGGACTC CGAGCATTCT 
GACTTGACCG AAGCTGTTGT TCGGAATCTA GCAACACATC TTTATCTTCC TGGGCTTTTT 
TATAGAGTTA CTGATGAAAA TGCCAAAAAT AGTGAACTTA ACCTTGTTGG TATGATCTGC 
TACACCAGCC AACATTATTG TGCCTTTGCA TTTCACACCA AAAGTTCCAA ATGGGTATTT 
TTTGATGATG CAAATGTGAA AGAGATTGGA ACTAGATGGA AAGATGTTGT CTCCAAATGC 
ATTCGATGCC ACTTTCAGCC ACTACTTTTG TTTTATGCAA ACCCAGATGG CACAGCAGTT 
TCTACTGAGG ATGCACTCAG GCAGGTCATC AGCTGGTCAC ATTACAAATC TGTTGCAGAA 
AATATGGGAT GTGAAAAGCC TGTAATTCAT AAGTCAGATA ATTTAAAAGA AAATGGATTT 
GGTGATCAGG CAAAGCAGAG AGAAAATCAG AAATTTCCAA CTGATAATAT TTCATCATCT 
AATCGGAGCC ACAGTCACAC AGGTGTAGGG AAAGGACCAG CTAAGTTAAG TCACATTGAT 
CAAAGGGAAA AGATAAAAGA CATTTCCAGA GAATGTGCTC TGAAAGCTAT TGAACAGAAA 
AACTTACTTT CTTCACAAAG GAAAGATTTA GAGAAGGGAC AAAGAAAAGA TTTAGGACGA 
CATAGAGATT TGGTTGATGA AGACCTTTCA CATTTCCAAT CTGGATCACC TCCTGCCCCA 
AATGGTTTTA AACAACATGG GAATCCACAT CTATATCATA GTCAAGGAAA AGGATCATAT 
AAACATGACC GAGTTGTACC TCAGAGTCGA GCTTCTGCAC AAATAATAAG TTCAAGTAAA 
TCCCAGATTC TTGCTCCAGG AGAGAAAATA ACTGGCAAAG TTAAGAGTGA CAATGGCACT 
GGATATGACA CAGACAGCAG CCAAGATTCT AGGGATAGAG GAAACAGCTG TGATAGCAGC 
AGTAAAAGCC GGAACCGAGG TTGGAAACCT ATGAGAGAAA CATTAAATGT TGATAGTATT 
TTTAGTGAAA GTGAAAAAAG ACAGCATAGT CCAAGACATA AACCAAATAT CAGTAATAAG 
CCTAAATCTA GCAAGGATCC GAGTTTTAGT AATTGGCCAA AAGAGAATCC AAAGCAAAAA 
GGTTTAATGA CCATATATGA AGATGAAATG AAGCAGGAAA TAGGAAGCAG AAGTTCCCTT 
GAATCTAATG GAAAAGGAGC AGAGAAAAAT AAAGGCCTTG TAGAGGGTAA AGTGCATGGT 
GATAATTGGC AGATGCAAAG GACTGAGTCT GGATATGAAA GCAGTGATCA CATCAGTAAT 
GGTTCTACTA ATTTGGACTC ACCTGTTATC GATGGAAATG GTACAGTAAT GGATATCAGT 
GGTGTTAAAG AAACAGTATG CTTCAGTGAC CAGATTACGA CAAGCAACCT AAATAAAGAA 
CGTGGGGACT GTACCTCCCT TCAGAGCCAA CATCACTTAG AAGGCTTTAG AAAAGAACTC 
AGGAATTTGG AAGCAGGCTA TAAATCTCAT GAATTCCACC CAGAATCACA TTTACAAATA 
AAAAATCATT TGATAAAAAG ATCACATGTA CATGAAGACA ATGGAAAGTT ATTTCCTTCA 
TCCAGTCTAC AAATACCCAA GGACCATAAT GCAAGAGAAC ATATCCACCA GTCAGATGAA 
CAGAAACTTG AAAAACCGAA TGAATGCAAA TTTTCTGAGT GGCTTAATAT AGAAAATTCT 
GAGAGAACAG GTTTGCCTTT TCACGTTGAT AACTCTGCTT CTGGGAAGAG AGTGAACAGT 
AATGAACCAT CTTCATTATG GTCTTCACAC CTAAGAACTG TTGGGTTAAA GCCAGAAACT 
GCTCCTCTCA TCCAGCAACA AAATATCATG GATCAATGTT ACTTTGAGAA CTCTCTATCC 
ACAGAATGTA TAATTCGGTC AGCCAGCAGA TCTGATGGGT GTCAGATGCC AAAACTTTTT 
TGCCAGAATC TACCACCCCC TTTGCCACCA AAGAAATATG CTATAACCAG TGTGCCACAG 
TCAGAGAAAA GCGAATCTAC ACCTGATGTC AAACTTACAG AGGTGTTTAA AGCTACCTCT 
CATCTTCCGA AGCACAGTTT AAGTACAGCT TCAGAACCAA GTTTAGAAGT GAGTACACAT 
ATGAATGATG AAAGACATAA AGAAACATTT CAAGTGAGAG AATGTTTTGG CAACACACCA 
AACTGTCCAT CCAGCTCCTC AACTAACGAT TTTCAGGCAA ACTCAGGTGC CATTGATGCA 
TTTTGCCAAC CAGAACTAGA CTCTATTTCT ACCTGTCCAA ATGAGACAGT TTCATTAACT 
ACCTATTTTT CAGTTGATAG CTGCATGACG GATACATATA GATTGAAATA CCATCAGAGG 
CCCAAGCTCT CTTTTCCAGA GAGCAGTGGC TTTTGTAATA ATTCACTATC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( XM_011532038.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530340.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu38976
Clone ID Related Accession (Same CDS sequence)	XM_006714240.1 , XM_011532038.2
Accession Version	XM_011532038.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532038.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATG CTGCGGAGTG CTTTGAAAAT ATGTTGGAGA GGATTCATTT TCACATAGTG 
CCAAGCAGAG ATGCAGACAT GTGTACCTCT AAATCTTGTA TCACTCACCA GAAGTTTGCT 
ATGACTCTGT ATGAACAGTG TGTGTGTCGT AGCTGTGGAG CATCGTCAGA TCCTCTACCT 
TTTACAGAAT TTGTGCGGTA CATTTCTACA ACAGCCTTAT GCAATGAGGT TGAAAGAATG 
TTGGAAAGGC ATGAACGCTT TAAACCTGAA ATGTTTGCAG AATTGCTACA AGCAGCAAAT 
ACAACAGATG ACTATAGGAA ATGTCCTAGT AACTGTGGCC AAAAAATAAA AATTCGCCGT 
GTTTTAATGA ATTGCCCAGA GATTGTTACA ATTGGTTTAG TCTGGGACTC CGAGCATTCT 
GACTTGACCG AAGCTGTTGT TCGGAATCTA GCAACACATC TTTATCTTCC TGGGCTTTTT 
TATAGAGTTA CTGATGAAAA TGCCAAAAAT AGTGAACTTA ACCTTGTTGG TATGATCTGC 
TACACCAGCC AACATTATTG TGCCTTTGCA TTTCACACCA AAAGTTCCAA ATGGGTATTT 
TTTGATGATG CAAATGTGAA AGAGATTGGA ACTAGATGGA AAGATGTTGT CTCCAAATGC 
ATTCGATGCC ACTTTCAGCC ACTACTTTTG TTTTATGCAA ACCCAGATGG CACAGCAGTT 
TCTACTGAGG ATGCACTCAG GCAGGTCATC AGCTGGTCAC ATTACAAATC TGTTGCAGAA 
AATATGGGAT GTGAAAAGCC TGTAATTCAT AAGTCAGATA ATTTAAAAGA AAATGGATTT 
GGTGATCAGG CAAAGCAGAG AGAAAATCAG AAATTTCCAA CTGATAATAT TTCATCATCT 
AATCGGAGCC ACAGTCACAC AGGTGTAGGG AAAGGACCAG CTAAGTTAAG TCACATTGAT 
CAAAGGGAAA AGATAAAAGA CATTTCCAGA GAATGTGCTC TGAAAGCTAT TGAACAGAAA 
AACTTACTTT CTTCACAAAG GAAAGATTTA GAGAAGGGAC AAAGAAAAGA TTTAGGACGA 
CATAGAGATT TGGTTGATGA AGACCTTTCA CATTTCCAAT CTGGATCACC TCCTGCCCCA 
AATGGTTTTA AACAACATGG GAATCCACAT CTATATCATA GTCAAGGAAA AGGATCATAT 
AAACATGACC GAGTTGTACC TCAGAGTCGA GCTTCTGCAC AAATAATAAG TTCAAGTAAA 
TCCCAGATTC TTGCTCCAGG AGAGAAAATA ACTGGCAAAG TTAAGAGTGA CAATGGCACT 
GGATATGACA CAGACAGCAG CCAAGATTCT AGGGATAGAG GAAACAGCTG TGATAGCAGC 
AGTAAAAGCC GGAACCGAGG TTGGAAACCT ATGAGAGAAA CATTAAATGT TGATAGTATT 
TTTAGTGAAA GTGAAAAAAG ACAGCATAGT CCAAGACATA AACCAAATAT CAGTAATAAG 
CCTAAATCTA GCAAGGATCC GAGTTTTAGT AATTGGCCAA AAGAGAATCC AAAGCAAAAA 
GGTTTAATGA CCATATATGA AGATGAAATG AAGCAGGAAA TAGGAAGCAG AAGTTCCCTT 
GAATCTAATG GAAAAGGAGC AGAGAAAAAT AAAGGCCTTG TAGAGGGTAA AGTGCATGGT 
GATAATTGGC AGATGCAAAG GACTGAGTCT GGATATGAAA GCAGTGATCA CATCAGTAAT 
GGTTCTACTA ATTTGGACTC ACCTGTTATC GATGGAAATG GTACAGTAAT GGATATCAGT 
GGTGTTAAAG AAACAGTATG CTTCAGTGAC CAGATTACGA CAAGCAACCT AAATAAAGAA 
CGTGGGGACT GTACCTCCCT TCAGAGCCAA CATCACTTAG AAGGCTTTAG AAAAGAACTC 
AGGAATTTGG AAGCAGGCTA TAAATCTCAT GAATTCCACC CAGAATCACA TTTACAAATA 
AAAAATCATT TGATAAAAAG ATCACATGTA CATGAAGACA ATGGAAAGTT ATTTCCTTCA 
TCCAGTCTAC AAATACCCAA GGACCATAAT GCAAGAGAAC ATATCCACCA GTCAGATGAA 
CAGAAACTTG AAAAACCGAA TGAATGCAAA TTTTCTGAGT GGCTTAATAT AGAAAATTCT 
GAGAGAACAG GTTTGCCTTT TCACGTTGAT AACTCTGCTT CTGGGAAGAG AGTGAACAGT 
AATGAACCAT CTTCATTATG GTCTTCACAC CTAAGAACTG TTGGGTTAAA GCCAGAAACT 
GCTCCTCTCA TCCAGCAACA AAATATCATG GATCAATGTT ACTTTGAGAA CTCTCTATCC 
ACAGAATGTA TAATTCGGTC AGCCAGCAGA TCTGATGGGT GTCAGATGCC AAAACTTTTT 
TGCCAGAATC TACCACCCCC TTTGCCACCA AAGAAATATG CTATAACCAG TGTGCCACAG 
TCAGAGAAAA GCGAATCTAC ACCTGATGTC AAACTTACAG AGGTGTTTAA AGCTACCTCT 
CATCTTCCGA AGCACAGTTT AAGTACAGCT TCAGAACCAA GTTTAGAAGT GAGTACACAT 
ATGAATGATG AAAGACATAA AGAAACATTT CAAGTGAGAG AATGTTTTGG CAACACACCA 
AACTGTCCAT CCAGCTCCTC AACTAACGAT TTTCAGGCAA ACTCAGGTGC CATTGATGCA 
TTTTGCCAAC CAGAACTAGA CTCTATTTCT ACCTGTCCAA ATGAGACAGT TTCATTAACT 
ACCTATTTTT CAGTTGATAG CTGCATGACG GATACATATA GATTGAAATA CCATCAGAGG 
CCCAAGCTCT CTTTTCCAGA GAGCAGTGGC TTTTGTAATA ATTCACTATC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530340.1
CDS	744..3617
Translation	MDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLKENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEGFRKELRNLEAGYKSHEFHPESHLQIKNHLIKRSHVHEDNGKLFPSSSLQIPKDHNAREHIHQSDEQKLEKPNECKFSEWLNIENSERTGLPFHVDNSASGKRVNSNEPSSLWSSHLRTVGLKPETAPLIQQQNIMDQCYFENSLSTECIIRSASRSDGCQMPKLFCQNLPPPLPPKKYAITSVPQSEKSESTPDVKLTEVFKATSHLPKHSLSTASEPSLEVSTHMNDERHKETFQVRECFGNTPNCPSSSSTNDFQANSGAIDAFCQPELDSISTCPNETVSLTTYFSVDSCMTDTYRLKYHQRPKLSFPESSGFCNNSLS

Target ORF information:

RefSeq Version	XM_011532038.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532038.2

ORF Insert Sequence:

ATGGATGATG CTGCGGAGTG CTTTGAAAAT ATGTTGGAGA GGATTCATTT TCACATAGTG 
CCAAGCAGAG ATGCAGACAT GTGTACCTCT AAATCTTGTA TCACTCACCA GAAGTTTGCT 
ATGACTCTGT ATGAACAGTG TGTGTGTCGT AGCTGTGGAG CATCGTCAGA TCCTCTACCT 
TTTACAGAAT TTGTGCGGTA CATTTCTACA ACAGCCTTAT GCAATGAGGT TGAAAGAATG 
TTGGAAAGGC ATGAACGCTT TAAACCTGAA ATGTTTGCAG AATTGCTACA AGCAGCAAAT 
ACAACAGATG ACTATAGGAA ATGTCCTAGT AACTGTGGCC AAAAAATAAA AATTCGCCGT 
GTTTTAATGA ATTGCCCAGA GATTGTTACA ATTGGTTTAG TCTGGGACTC CGAGCATTCT 
GACTTGACCG AAGCTGTTGT TCGGAATCTA GCAACACATC TTTATCTTCC TGGGCTTTTT 
TATAGAGTTA CTGATGAAAA TGCCAAAAAT AGTGAACTTA ACCTTGTTGG TATGATCTGC 
TACACCAGCC AACATTATTG TGCCTTTGCA TTTCACACCA AAAGTTCCAA ATGGGTATTT 
TTTGATGATG CAAATGTGAA AGAGATTGGA ACTAGATGGA AAGATGTTGT CTCCAAATGC 
ATTCGATGCC ACTTTCAGCC ACTACTTTTG TTTTATGCAA ACCCAGATGG CACAGCAGTT 
TCTACTGAGG ATGCACTCAG GCAGGTCATC AGCTGGTCAC ATTACAAATC TGTTGCAGAA 
AATATGGGAT GTGAAAAGCC TGTAATTCAT AAGTCAGATA ATTTAAAAGA AAATGGATTT 
GGTGATCAGG CAAAGCAGAG AGAAAATCAG AAATTTCCAA CTGATAATAT TTCATCATCT 
AATCGGAGCC ACAGTCACAC AGGTGTAGGG AAAGGACCAG CTAAGTTAAG TCACATTGAT 
CAAAGGGAAA AGATAAAAGA CATTTCCAGA GAATGTGCTC TGAAAGCTAT TGAACAGAAA 
AACTTACTTT CTTCACAAAG GAAAGATTTA GAGAAGGGAC AAAGAAAAGA TTTAGGACGA 
CATAGAGATT TGGTTGATGA AGACCTTTCA CATTTCCAAT CTGGATCACC TCCTGCCCCA 
AATGGTTTTA AACAACATGG GAATCCACAT CTATATCATA GTCAAGGAAA AGGATCATAT 
AAACATGACC GAGTTGTACC TCAGAGTCGA GCTTCTGCAC AAATAATAAG TTCAAGTAAA 
TCCCAGATTC TTGCTCCAGG AGAGAAAATA ACTGGCAAAG TTAAGAGTGA CAATGGCACT 
GGATATGACA CAGACAGCAG CCAAGATTCT AGGGATAGAG GAAACAGCTG TGATAGCAGC 
AGTAAAAGCC GGAACCGAGG TTGGAAACCT ATGAGAGAAA CATTAAATGT TGATAGTATT 
TTTAGTGAAA GTGAAAAAAG ACAGCATAGT CCAAGACATA AACCAAATAT CAGTAATAAG 
CCTAAATCTA GCAAGGATCC GAGTTTTAGT AATTGGCCAA AAGAGAATCC AAAGCAAAAA 
GGTTTAATGA CCATATATGA AGATGAAATG AAGCAGGAAA TAGGAAGCAG AAGTTCCCTT 
GAATCTAATG GAAAAGGAGC AGAGAAAAAT AAAGGCCTTG TAGAGGGTAA AGTGCATGGT 
GATAATTGGC AGATGCAAAG GACTGAGTCT GGATATGAAA GCAGTGATCA CATCAGTAAT 
GGTTCTACTA ATTTGGACTC ACCTGTTATC GATGGAAATG GTACAGTAAT GGATATCAGT 
GGTGTTAAAG AAACAGTATG CTTCAGTGAC CAGATTACGA CAAGCAACCT AAATAAAGAA 
CGTGGGGACT GTACCTCCCT TCAGAGCCAA CATCACTTAG AAGGCTTTAG AAAAGAACTC 
AGGAATTTGG AAGCAGGCTA TAAATCTCAT GAATTCCACC CAGAATCACA TTTACAAATA 
AAAAATCATT TGATAAAAAG ATCACATGTA CATGAAGACA ATGGAAAGTT ATTTCCTTCA 
TCCAGTCTAC AAATACCCAA GGACCATAAT GCAAGAGAAC ATATCCACCA GTCAGATGAA 
CAGAAACTTG AAAAACCGAA TGAATGCAAA TTTTCTGAGT GGCTTAATAT AGAAAATTCT 
GAGAGAACAG GTTTGCCTTT TCACGTTGAT AACTCTGCTT CTGGGAAGAG AGTGAACAGT 
AATGAACCAT CTTCATTATG GTCTTCACAC CTAAGAACTG TTGGGTTAAA GCCAGAAACT 
GCTCCTCTCA TCCAGCAACA AAATATCATG GATCAATGTT ACTTTGAGAA CTCTCTATCC 
ACAGAATGTA TAATTCGGTC AGCCAGCAGA TCTGATGGGT GTCAGATGCC AAAACTTTTT 
TGCCAGAATC TACCACCCCC TTTGCCACCA AAGAAATATG CTATAACCAG TGTGCCACAG 
TCAGAGAAAA GCGAATCTAC ACCTGATGTC AAACTTACAG AGGTGTTTAA AGCTACCTCT 
CATCTTCCGA AGCACAGTTT AAGTACAGCT TCAGAACCAA GTTTAGAAGT GAGTACACAT 
ATGAATGATG AAAGACATAA AGAAACATTT CAAGTGAGAG AATGTTTTGG CAACACACCA 
AACTGTCCAT CCAGCTCCTC AACTAACGAT TTTCAGGCAA ACTCAGGTGC CATTGATGCA 
TTTTGCCAAC CAGAACTAGA CTCTATTTCT ACCTGTCCAA ATGAGACAGT TTCATTAACT 
ACCTATTTTT CAGTTGATAG CTGCATGACG GATACATATA GATTGAAATA CCATCAGAGG 
CCCAAGCTCT CTTTTCCAGA GAGCAGTGGC TTTTGTAATA ATTCACTATC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( XM_017008318.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863807.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu38977
Clone ID Related Accession (Same CDS sequence)	XM_017008318.2 , XM_005263077.3 , XM_017008318.1 , NM_001371398.1 , XM_017008317.1 , XM_005263077.4 , XM_017008317.2 , NM_001371397.1
Accession Version	XM_017008318.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017008318.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT 
TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC 
AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863807.1
CDS	1051..3453
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLKENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEDHMYMKTMESYFLHPVYKYPRTIMQENISTSQMNRNLKNRMNANFLSGLI

Target ORF information:

RefSeq Version	XM_017008318.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008318.2

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT 
TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC 
AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( XM_005263077.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263134.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OHu38977
Clone ID Related Accession (Same CDS sequence)	XM_017008318.2 , XM_005263077.3 , XM_017008318.1 , NM_001371398.1 , XM_017008317.1 , XM_005263077.4 , XM_017008317.2 , NM_001371397.1
Accession Version	XM_005263077.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263077.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT 
TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC 
AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263134.1
CDS	1106..3508
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLKENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEDHMYMKTMESYFLHPVYKYPRTIMQENISTSQMNRNLKNRMNANFLSGLI

Target ORF information:

RefSeq Version	XM_005263077.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263077.3

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT 
TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC 
AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( XM_017008318.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863807.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu38977
Clone ID Related Accession (Same CDS sequence)	XM_017008318.2 , XM_005263077.3 , XM_017008318.1 , NM_001371398.1 , XM_017008317.1 , XM_005263077.4 , XM_017008317.2 , NM_001371397.1
Accession Version	XM_017008318.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT 
TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC 
AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863807.1
CDS	1052..3454
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLKENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEDHMYMKTMESYFLHPVYKYPRTIMQENISTSQMNRNLKNRMNANFLSGLI

Target ORF information:

RefSeq Version	XM_017008318.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008318.1

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT 
TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC 
AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( XM_017008317.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863806.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu38977
Clone ID Related Accession (Same CDS sequence)	XM_017008318.2 , XM_005263077.3 , XM_017008318.1 , NM_001371398.1 , XM_017008317.1 , XM_005263077.4 , XM_017008317.2 , NM_001371397.1
Accession Version	XM_017008317.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT 
TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC 
AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863806.1
CDS	930..3332
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLKENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEDHMYMKTMESYFLHPVYKYPRTIMQENISTSQMNRNLKNRMNANFLSGLI

Target ORF information:

RefSeq Version	XM_017008317.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008317.1

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT 
TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC 
AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( XM_005263077.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263134.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OHu38977
Clone ID Related Accession (Same CDS sequence)	XM_017008318.2 , XM_005263077.3 , XM_017008318.1 , NM_001371398.1 , XM_017008317.1 , XM_005263077.4 , XM_017008317.2 , NM_001371397.1
Accession Version	XM_005263077.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005263077.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT 
TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC 
AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263134.1
CDS	1067..3469
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLKENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEDHMYMKTMESYFLHPVYKYPRTIMQENISTSQMNRNLKNRMNANFLSGLI

Target ORF information:

RefSeq Version	XM_005263077.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263077.4

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT 
TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC 
AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( XM_017008317.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863806.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu38977
Clone ID Related Accession (Same CDS sequence)	XM_017008318.2 , XM_005263077.3 , XM_017008318.1 , NM_001371398.1 , XM_017008317.1 , XM_005263077.4 , XM_017008317.2 , NM_001371397.1
Accession Version	XM_017008317.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017008317.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT 
TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC 
AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863806.1
CDS	899..3301
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLKENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEDHMYMKTMESYFLHPVYKYPRTIMQENISTSQMNRNLKNRMNANFLSGLI

Target ORF information:

RefSeq Version	XM_017008317.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008317.2

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT 
TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC 
AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( NM_001371398.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001358327.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu38977
Clone ID Related Accession (Same CDS sequence)	XM_017008318.2 , XM_005263077.3 , XM_017008318.1 , NM_001371398.1 , XM_017008317.1 , XM_005263077.4 , XM_017008317.2 , NM_001371397.1
Accession Version	NM_001371398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-10
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092656.1. On Jul 11, 2019 this sequence version replaced XM_017008318.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.29668.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT 
TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC 
AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001358327.1
CDS	950..3352
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLKENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEDHMYMKTMESYFLHPVYKYPRTIMQENISTSQMNRNLKNRMNANFLSGLI

Target ORF information:

RefSeq Version	NM_001371398.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001371398.1

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT 
TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC 
AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( NM_001371397.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001358326.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu38977
Clone ID Related Accession (Same CDS sequence)	XM_017008318.2 , XM_005263077.3 , XM_017008318.1 , NM_001371398.1 , XM_017008317.1 , XM_005263077.4 , XM_017008317.2 , NM_001371397.1
Accession Version	NM_001371397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-10
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092656.1. On Jul 11, 2019 this sequence version replaced XM_017008317.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.80818.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT 
TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC 
AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001358326.1
CDS	828..3230
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLKENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEDHMYMKTMESYFLHPVYKYPRTIMQENISTSQMNRNLKNRMNANFLSGLI

Target ORF information:

RefSeq Version	NM_001371397.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001371397.1

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GATCACATGT ACATGAAGAC AATGGAAAGT 
TATTTCCTTC ATCCAGTCTA CAAATACCCA AGGACCATAA TGCAAGAGAA CATATCCACC 
AGTCAGATGA ACAGAAACTT GAAAAACCGA ATGAATGCAA ATTTTCTGAG TGGCTTAATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( NM_001371395.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001358324.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02420
Clone ID Related Accession (Same CDS sequence)	XM_017008312.2 , XM_005263073.4 , XM_005263073.3 , NM_001371395.1 , XM_017008313.1 , XM_011532037.2 , XM_017008314.1 , XM_017008312.1 , XM_017008315.1 , NM_001371399.1 , NM_019050.2 , XM_017008315.2
Accession Version	NM_001371395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-10
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092656.1. On Jul 11, 2019 this sequence version replaced XM_017008312.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: CR749490.1, SRR1660803.241937.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA 
GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG 
ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA 
ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA 
AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT 
TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT 
TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC 
CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA 
ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA 
CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC 
GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG 
CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA 
AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC 
AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA 
GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA 
GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT 
TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001358324.1
CDS	1103..4324
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLKENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEGFRKELRNLEAGYKSHEFHPESHLQIKNHLIKRSHVHEDNGKLFPSSSLQIPKDHNAREHIHQSDEQKLEKPNECKFSEWLNIENSERTGLPFHVDNSASGKRVNSNEPSSLWSSHLRTVGLKPETAPLIQQQNIMDQCYFENSLSTECIIRSASRSDGCQMPKLFCQNLPPPLPPKKYAITSVPQSEKSESTPDVKLTEVFKATSHLPKHSLSTASEPSLEVSTHMNDERHKETFQVRECFGNTPNCPSSSSTNDFQANSGAIDAFCQPELDSISTCPNETVSLTTYFSVDSCMTDTYRLKYHQRPKLSFPESSGFCNNSLS

Target ORF information:

RefSeq Version	NM_001371395.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001371395.1

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA 
GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG 
ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA 
ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA 
AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT 
TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT 
TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC 
CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA 
ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA 
CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC 
GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG 
CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA 
AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC 
AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA 
GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA 
GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT 
TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( NM_001371399.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001358328.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu02420
Clone ID Related Accession (Same CDS sequence)	XM_017008312.2 , XM_005263073.4 , XM_005263073.3 , NM_001371395.1 , XM_017008313.1 , XM_011532037.2 , XM_017008314.1 , XM_017008312.1 , XM_017008315.1 , NM_001371399.1 , NM_019050.2 , XM_017008315.2
Accession Version	NM_001371399.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-10
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092656.1. On Jul 11, 2019 this sequence version replaced XM_005263073.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.100920.1, SRR1803611.239951.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA 
GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG 
ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA 
ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA 
AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT 
TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT 
TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC 
CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA 
ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA 
CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC 
GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG 
CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA 
AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC 
AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA 
GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA 
GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT 
TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001358328.1
CDS	828..4049
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLKENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEGFRKELRNLEAGYKSHEFHPESHLQIKNHLIKRSHVHEDNGKLFPSSSLQIPKDHNAREHIHQSDEQKLEKPNECKFSEWLNIENSERTGLPFHVDNSASGKRVNSNEPSSLWSSHLRTVGLKPETAPLIQQQNIMDQCYFENSLSTECIIRSASRSDGCQMPKLFCQNLPPPLPPKKYAITSVPQSEKSESTPDVKLTEVFKATSHLPKHSLSTASEPSLEVSTHMNDERHKETFQVRECFGNTPNCPSSSSTNDFQANSGAIDAFCQPELDSISTCPNETVSLTTYFSVDSCMTDTYRLKYHQRPKLSFPESSGFCNNSLS

Target ORF information:

RefSeq Version	NM_001371399.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001371399.1

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA 
GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG 
ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA 
ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA 
AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT 
TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT 
TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC 
CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA 
ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA 
CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC 
GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG 
CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA 
AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC 
AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA 
GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA 
GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT 
TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( NM_001371396.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001358325.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu38975
Clone ID Related Accession (Same CDS sequence)	NM_001371396.1 , XM_005263076.2 , XM_017008316.1 , XM_017008316.2 , XM_005263076.3
Accession Version	NM_001371396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-10
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092656.1. On Jul 11, 2019 this sequence version replaced XM_017008316.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.74097.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGCTAAG 
TTAAGTCACA TTGATCAAAG GGAAAAGATA AAAGACATTT CCAGAGAATG TGCTCTGAAA 
GCTATTGAAC AGAAAAACTT ACTTTCTTCA CAAAGGAAAG ATTTAGAGAA GGGACAAAGA 
AAAGATTTAG GACGACATAG AGATTTGGTT GATGAAGACC TTTCACATTT CCAATCTGGA 
TCACCTCCTG CCCCAAATGG TTTTAAACAA CATGGGAATC CACATCTATA TCATAGTCAA 
GGAAAAGGAT CATATAAACA TGACCGAGTT GTACCTCAGA GTCGAGCTTC TGCACAAATA 
ATAAGTTCAA GTAAATCCCA GATTCTTGCT CCAGGAGAGA AAATAACTGG CAAAGTTAAG 
AGTGACAATG GCACTGGATA TGACACAGAC AGCAGCCAAG ATTCTAGGGA TAGAGGAAAC 
AGCTGTGATA GCAGCAGTAA AAGCCGGAAC CGAGGTTGGA AACCTATGAG AGAAACATTA 
AATGTTGATA GTATTTTTAG TGAAAGTGAA AAAAGACAGC ATAGTCCAAG ACATAAACCA 
AATATCAGTA ATAAGCCTAA ATCTAGCAAG GATCCGAGTT TTAGTAATTG GCCAAAAGAG 
AATCCAAAGC AAAAAGGTTT AATGACCATA TATGAAGATG AAATGAAGCA GGAAATAGGA 
AGCAGAAGTT CCCTTGAATC TAATGGAAAA GGAGCAGAGA AAAATAAAGG CCTTGTAGAG 
GGTAAAGTGC ATGGTGATAA TTGGCAGATG CAAAGGACTG AGTCTGGATA TGAAAGCAGT 
GATCACATCA GTAATGGTTC TACTAATTTG GACTCACCTG TTATCGATGG AAATGGTACA 
GTAATGGATA TCAGTGGTGT TAAAGAAACA GTATGCTTCA GTGACCAGAT TACGACAAGC 
AACCTAAATA AAGAACGTGG GGACTGTACC TCCCTTCAGA GCCAACATCA CTTAGAAGGC 
TTTAGAAAAG AACTCAGGAA TTTGGAAGCA GGCTATAAAT CTCATGAATT CCACCCAGAA 
TCACATTTAC AAATAAAAAA TCATTTGATA AAAAGATCAC ATGTACATGA AGACAATGGA 
AAGTTATTTC CTTCATCCAG TCTACAAATA CCCAAGGACC ATAATGCAAG AGAACATATC 
CACCAGTCAG ATGAACAGAA ACTTGAAAAA CCGAATGAAT GCAAATTTTC TGAGTGGCTT 
AATATAGAAA ATTCTGAGAG AACAGGTTTG CCTTTTCACG TTGATAACTC TGCTTCTGGG 
AAGAGAGTGA ACAGTAATGA ACCATCTTCA TTATGGTCTT CACACCTAAG AACTGTTGGG 
TTAAAGCCAG AAACTGCTCC TCTCATCCAG CAACAAAATA TCATGGATCA ATGTTACTTT 
GAGAACTCTC TATCCACAGA ATGTATAATT CGGTCAGCCA GCAGATCTGA TGGGTGTCAG 
ATGCCAAAAC TTTTTTGCCA GAATCTACCA CCCCCTTTGC CACCAAAGAA ATATGCTATA 
ACCAGTGTGC CACAGTCAGA GAAAAGCGAA TCTACACCTG ATGTCAAACT TACAGAGGTG 
TTTAAAGCTA CCTCTCATCT TCCGAAGCAC AGTTTAAGTA CAGCTTCAGA ACCAAGTTTA 
GAAGTGAGTA CACATATGAA TGATGAAAGA CATAAAGAAA CATTTCAAGT GAGAGAATGT 
TTTGGCAACA CACCAAACTG TCCATCCAGC TCCTCAACTA ACGATTTTCA GGCAAACTCA 
GGTGCCATTG ATGCATTTTG CCAACCAGAA CTAGACTCTA TTTCTACCTG TCCAAATGAG 
ACAGTTTCAT TAACTACCTA TTTTTCAGTT GATAGCTGCA TGACGGATAC ATATAGATTG 
AAATACCATC AGAGGCCCAA GCTCTCTTTT CCAGAGAGCA GTGGCTTTTG TAATAATTCA 
CTATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001358325.1
CDS	828..3896
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEGFRKELRNLEAGYKSHEFHPESHLQIKNHLIKRSHVHEDNGKLFPSSSLQIPKDHNAREHIHQSDEQKLEKPNECKFSEWLNIENSERTGLPFHVDNSASGKRVNSNEPSSLWSSHLRTVGLKPETAPLIQQQNIMDQCYFENSLSTECIIRSASRSDGCQMPKLFCQNLPPPLPPKKYAITSVPQSEKSESTPDVKLTEVFKATSHLPKHSLSTASEPSLEVSTHMNDERHKETFQVRECFGNTPNCPSSSSTNDFQANSGAIDAFCQPELDSISTCPNETVSLTTYFSVDSCMTDTYRLKYHQRPKLSFPESSGFCNNSLS

Target ORF information:

RefSeq Version	NM_001371396.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001371396.1

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGCTAAG 
TTAAGTCACA TTGATCAAAG GGAAAAGATA AAAGACATTT CCAGAGAATG TGCTCTGAAA 
GCTATTGAAC AGAAAAACTT ACTTTCTTCA CAAAGGAAAG ATTTAGAGAA GGGACAAAGA 
AAAGATTTAG GACGACATAG AGATTTGGTT GATGAAGACC TTTCACATTT CCAATCTGGA 
TCACCTCCTG CCCCAAATGG TTTTAAACAA CATGGGAATC CACATCTATA TCATAGTCAA 
GGAAAAGGAT CATATAAACA TGACCGAGTT GTACCTCAGA GTCGAGCTTC TGCACAAATA 
ATAAGTTCAA GTAAATCCCA GATTCTTGCT CCAGGAGAGA AAATAACTGG CAAAGTTAAG 
AGTGACAATG GCACTGGATA TGACACAGAC AGCAGCCAAG ATTCTAGGGA TAGAGGAAAC 
AGCTGTGATA GCAGCAGTAA AAGCCGGAAC CGAGGTTGGA AACCTATGAG AGAAACATTA 
AATGTTGATA GTATTTTTAG TGAAAGTGAA AAAAGACAGC ATAGTCCAAG ACATAAACCA 
AATATCAGTA ATAAGCCTAA ATCTAGCAAG GATCCGAGTT TTAGTAATTG GCCAAAAGAG 
AATCCAAAGC AAAAAGGTTT AATGACCATA TATGAAGATG AAATGAAGCA GGAAATAGGA 
AGCAGAAGTT CCCTTGAATC TAATGGAAAA GGAGCAGAGA AAAATAAAGG CCTTGTAGAG 
GGTAAAGTGC ATGGTGATAA TTGGCAGATG CAAAGGACTG AGTCTGGATA TGAAAGCAGT 
GATCACATCA GTAATGGTTC TACTAATTTG GACTCACCTG TTATCGATGG AAATGGTACA 
GTAATGGATA TCAGTGGTGT TAAAGAAACA GTATGCTTCA GTGACCAGAT TACGACAAGC 
AACCTAAATA AAGAACGTGG GGACTGTACC TCCCTTCAGA GCCAACATCA CTTAGAAGGC 
TTTAGAAAAG AACTCAGGAA TTTGGAAGCA GGCTATAAAT CTCATGAATT CCACCCAGAA 
TCACATTTAC AAATAAAAAA TCATTTGATA AAAAGATCAC ATGTACATGA AGACAATGGA 
AAGTTATTTC CTTCATCCAG TCTACAAATA CCCAAGGACC ATAATGCAAG AGAACATATC 
CACCAGTCAG ATGAACAGAA ACTTGAAAAA CCGAATGAAT GCAAATTTTC TGAGTGGCTT 
AATATAGAAA ATTCTGAGAG AACAGGTTTG CCTTTTCACG TTGATAACTC TGCTTCTGGG 
AAGAGAGTGA ACAGTAATGA ACCATCTTCA TTATGGTCTT CACACCTAAG AACTGTTGGG 
TTAAAGCCAG AAACTGCTCC TCTCATCCAG CAACAAAATA TCATGGATCA ATGTTACTTT 
GAGAACTCTC TATCCACAGA ATGTATAATT CGGTCAGCCA GCAGATCTGA TGGGTGTCAG 
ATGCCAAAAC TTTTTTGCCA GAATCTACCA CCCCCTTTGC CACCAAAGAA ATATGCTATA 
ACCAGTGTGC CACAGTCAGA GAAAAGCGAA TCTACACCTG ATGTCAAACT TACAGAGGTG 
TTTAAAGCTA CCTCTCATCT TCCGAAGCAC AGTTTAAGTA CAGCTTCAGA ACCAAGTTTA 
GAAGTGAGTA CACATATGAA TGATGAAAGA CATAAAGAAA CATTTCAAGT GAGAGAATGT 
TTTGGCAACA CACCAAACTG TCCATCCAGC TCCTCAACTA ACGATTTTCA GGCAAACTCA 
GGTGCCATTG ATGCATTTTG CCAACCAGAA CTAGACTCTA TTTCTACCTG TCCAAATGAG 
ACAGTTTCAT TAACTACCTA TTTTTCAGTT GATAGCTGCA TGACGGATAC ATATAGATTG 
AAATACCATC AGAGGCCCAA GCTCTCTTTT CCAGAGAGCA GTGGCTTTTG TAATAATTCA 
CTATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( NM_019050.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_061923.2 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02420
Clone ID Related Accession (Same CDS sequence)	XM_017008312.2 , XM_005263073.4 , XM_005263073.3 , NM_001371395.1 , XM_017008313.1 , XM_011532037.2 , XM_017008314.1 , XM_017008312.1 , XM_017008315.1 , NM_001371399.1 , NM_019050.2 , XM_017008315.2
Accession Version	NM_019050.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA886370.1, DA859085.1, CR749490.1, AB037771.1, BX648339.1, AK022799.1, CD721951.1 and BQ019723.1. On Jun 2, 2007 this sequence version replaced NM_019050.1. ##Evidence-Data-START## CDS exon combination :: SRR1660807.47065.1, SRR1660803.100920.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA 
GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG 
ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA 
ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA 
AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT 
TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT 
TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC 
CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA 
ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA 
CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC 
GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG 
CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA 
AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC 
AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA 
GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA 
GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT 
TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061923.2
CDS	1067..4288
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLKENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEGFRKELRNLEAGYKSHEFHPESHLQIKNHLIKRSHVHEDNGKLFPSSSLQIPKDHNAREHIHQSDEQKLEKPNECKFSEWLNIENSERTGLPFHVDNSASGKRVNSNEPSSLWSSHLRTVGLKPETAPLIQQQNIMDQCYFENSLSTECIIRSASRSDGCQMPKLFCQNLPPPLPPKKYAITSVPQSEKSESTPDVKLTEVFKATSHLPKHSLSTASEPSLEVSTHMNDERHKETFQVRECFGNTPNCPSSSSTNDFQANSGAIDAFCQPELDSISTCPNETVSLTTYFSVDSCMTDTYRLKYHQRPKLSFPESSGFCNNSLS

Target ORF information:

RefSeq Version	NM_019050.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019050.2

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA 
GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG 
ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA 
ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA 
AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT 
TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT 
TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC 
CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA 
ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA 
CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC 
GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG 
CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA 
AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC 
AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA 
GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA 
GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT 
TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( XM_017008312.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863801.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X1, mRNA.

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CloneID	OHu02420
Clone ID Related Accession (Same CDS sequence)	XM_017008312.2 , XM_005263073.4 , XM_005263073.3 , NM_001371395.1 , XM_017008313.1 , XM_011532037.2 , XM_017008314.1 , XM_017008312.1 , XM_017008315.1 , NM_001371399.1 , NM_019050.2 , XM_017008315.2
Accession Version	XM_017008312.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017008312.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA 
GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG 
ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA 
ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA 
AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT 
TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT 
TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC 
CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA 
ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA 
CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC 
GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG 
CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA 
AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC 
AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA 
GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA 
GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT 
TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863801.1
CDS	1233..4454
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLKENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEGFRKELRNLEAGYKSHEFHPESHLQIKNHLIKRSHVHEDNGKLFPSSSLQIPKDHNAREHIHQSDEQKLEKPNECKFSEWLNIENSERTGLPFHVDNSASGKRVNSNEPSSLWSSHLRTVGLKPETAPLIQQQNIMDQCYFENSLSTECIIRSASRSDGCQMPKLFCQNLPPPLPPKKYAITSVPQSEKSESTPDVKLTEVFKATSHLPKHSLSTASEPSLEVSTHMNDERHKETFQVRECFGNTPNCPSSSSTNDFQANSGAIDAFCQPELDSISTCPNETVSLTTYFSVDSCMTDTYRLKYHQRPKLSFPESSGFCNNSLS

Target ORF information:

RefSeq Version	XM_017008312.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008312.2

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA 
GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG 
ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA 
ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA 
AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT 
TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT 
TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC 
CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA 
ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA 
CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC 
GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG 
CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA 
AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC 
AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA 
GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA 
GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT 
TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( XM_005263073.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263130.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu02420
Clone ID Related Accession (Same CDS sequence)	XM_017008312.2 , XM_005263073.4 , XM_005263073.3 , NM_001371395.1 , XM_017008313.1 , XM_011532037.2 , XM_017008314.1 , XM_017008312.1 , XM_017008315.1 , NM_001371399.1 , NM_019050.2 , XM_017008315.2
Accession Version	XM_005263073.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005263073.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA 
GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG 
ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA 
ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA 
AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT 
TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT 
TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC 
CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA 
ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA 
CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC 
GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG 
CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA 
AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC 
AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA 
GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA 
GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT 
TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263130.1
CDS	899..4120
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLKENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEGFRKELRNLEAGYKSHEFHPESHLQIKNHLIKRSHVHEDNGKLFPSSSLQIPKDHNAREHIHQSDEQKLEKPNECKFSEWLNIENSERTGLPFHVDNSASGKRVNSNEPSSLWSSHLRTVGLKPETAPLIQQQNIMDQCYFENSLSTECIIRSASRSDGCQMPKLFCQNLPPPLPPKKYAITSVPQSEKSESTPDVKLTEVFKATSHLPKHSLSTASEPSLEVSTHMNDERHKETFQVRECFGNTPNCPSSSSTNDFQANSGAIDAFCQPELDSISTCPNETVSLTTYFSVDSCMTDTYRLKYHQRPKLSFPESSGFCNNSLS

Target ORF information:

RefSeq Version	XM_005263073.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263073.4

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA 
GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG 
ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA 
ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA 
AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT 
TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT 
TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC 
CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA 
ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA 
CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC 
GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG 
CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA 
AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC 
AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA 
GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA 
GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT 
TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( XM_005263073.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263130.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu02420
Clone ID Related Accession (Same CDS sequence)	XM_017008312.2 , XM_005263073.4 , XM_005263073.3 , NM_001371395.1 , XM_017008313.1 , XM_011532037.2 , XM_017008314.1 , XM_017008312.1 , XM_017008315.1 , NM_001371399.1 , NM_019050.2 , XM_017008315.2
Accession Version	XM_005263073.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263073.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA 
GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG 
ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA 
ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA 
AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT 
TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT 
TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC 
CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA 
ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA 
CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC 
GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG 
CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA 
AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC 
AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA 
GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA 
GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT 
TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263130.1
CDS	941..4162
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLKENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEGFRKELRNLEAGYKSHEFHPESHLQIKNHLIKRSHVHEDNGKLFPSSSLQIPKDHNAREHIHQSDEQKLEKPNECKFSEWLNIENSERTGLPFHVDNSASGKRVNSNEPSSLWSSHLRTVGLKPETAPLIQQQNIMDQCYFENSLSTECIIRSASRSDGCQMPKLFCQNLPPPLPPKKYAITSVPQSEKSESTPDVKLTEVFKATSHLPKHSLSTASEPSLEVSTHMNDERHKETFQVRECFGNTPNCPSSSSTNDFQANSGAIDAFCQPELDSISTCPNETVSLTTYFSVDSCMTDTYRLKYHQRPKLSFPESSGFCNNSLS

Target ORF information:

RefSeq Version	XM_005263073.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263073.3

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA 
GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG 
ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA 
ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA 
AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT 
TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT 
TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC 
CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA 
ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA 
CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC 
GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG 
CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA 
AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC 
AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA 
GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA 
GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT 
TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( XM_017008313.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863802.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu02420
Clone ID Related Accession (Same CDS sequence)	XM_017008312.2 , XM_005263073.4 , XM_005263073.3 , NM_001371395.1 , XM_017008313.1 , XM_011532037.2 , XM_017008314.1 , XM_017008312.1 , XM_017008315.1 , NM_001371399.1 , NM_019050.2 , XM_017008315.2
Accession Version	XM_017008313.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA 
GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG 
ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA 
ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA 
AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT 
TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT 
TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC 
CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA 
ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA 
CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC 
GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG 
CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA 
AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC 
AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA 
GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA 
GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT 
TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863802.1
CDS	918..4139
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLKENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEGFRKELRNLEAGYKSHEFHPESHLQIKNHLIKRSHVHEDNGKLFPSSSLQIPKDHNAREHIHQSDEQKLEKPNECKFSEWLNIENSERTGLPFHVDNSASGKRVNSNEPSSLWSSHLRTVGLKPETAPLIQQQNIMDQCYFENSLSTECIIRSASRSDGCQMPKLFCQNLPPPLPPKKYAITSVPQSEKSESTPDVKLTEVFKATSHLPKHSLSTASEPSLEVSTHMNDERHKETFQVRECFGNTPNCPSSSSTNDFQANSGAIDAFCQPELDSISTCPNETVSLTTYFSVDSCMTDTYRLKYHQRPKLSFPESSGFCNNSLS

Target ORF information:

RefSeq Version	XM_017008313.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008313.1

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA 
GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG 
ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA 
ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA 
AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT 
TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT 
TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC 
CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA 
ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA 
CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC 
GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG 
CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA 
AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC 
AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA 
GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA 
GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT 
TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( XM_011532037.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530339.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu02420
Clone ID Related Accession (Same CDS sequence)	XM_017008312.2 , XM_005263073.4 , XM_005263073.3 , NM_001371395.1 , XM_017008313.1 , XM_011532037.2 , XM_017008314.1 , XM_017008312.1 , XM_017008315.1 , NM_001371399.1 , NM_019050.2 , XM_017008315.2
Accession Version	XM_011532037.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532037.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA 
GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG 
ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA 
ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA 
AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT 
TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT 
TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC 
CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA 
ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA 
CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC 
GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG 
CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA 
AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC 
AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA 
GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA 
GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT 
TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530339.1
CDS	1119..4340
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLKENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEGFRKELRNLEAGYKSHEFHPESHLQIKNHLIKRSHVHEDNGKLFPSSSLQIPKDHNAREHIHQSDEQKLEKPNECKFSEWLNIENSERTGLPFHVDNSASGKRVNSNEPSSLWSSHLRTVGLKPETAPLIQQQNIMDQCYFENSLSTECIIRSASRSDGCQMPKLFCQNLPPPLPPKKYAITSVPQSEKSESTPDVKLTEVFKATSHLPKHSLSTASEPSLEVSTHMNDERHKETFQVRECFGNTPNCPSSSSTNDFQANSGAIDAFCQPELDSISTCPNETVSLTTYFSVDSCMTDTYRLKYHQRPKLSFPESSGFCNNSLS

Target ORF information:

RefSeq Version	XM_011532037.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532037.2

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA 
GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG 
ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA 
ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA 
AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT 
TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT 
TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC 
CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA 
ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA 
CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC 
GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG 
CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA 
AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC 
AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA 
GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA 
GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT 
TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( XM_017008314.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863803.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu02420
Clone ID Related Accession (Same CDS sequence)	XM_017008312.2 , XM_005263073.4 , XM_005263073.3 , NM_001371395.1 , XM_017008313.1 , XM_011532037.2 , XM_017008314.1 , XM_017008312.1 , XM_017008315.1 , NM_001371399.1 , NM_019050.2 , XM_017008315.2
Accession Version	XM_017008314.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA 
GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG 
ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA 
ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA 
AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT 
TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT 
TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC 
CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA 
ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA 
CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC 
GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG 
CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA 
AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC 
AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA 
GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA 
GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT 
TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863803.1
CDS	917..4138
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLKENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEGFRKELRNLEAGYKSHEFHPESHLQIKNHLIKRSHVHEDNGKLFPSSSLQIPKDHNAREHIHQSDEQKLEKPNECKFSEWLNIENSERTGLPFHVDNSASGKRVNSNEPSSLWSSHLRTVGLKPETAPLIQQQNIMDQCYFENSLSTECIIRSASRSDGCQMPKLFCQNLPPPLPPKKYAITSVPQSEKSESTPDVKLTEVFKATSHLPKHSLSTASEPSLEVSTHMNDERHKETFQVRECFGNTPNCPSSSSTNDFQANSGAIDAFCQPELDSISTCPNETVSLTTYFSVDSCMTDTYRLKYHQRPKLSFPESSGFCNNSLS

Target ORF information:

RefSeq Version	XM_017008314.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008314.1

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA 
GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG 
ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA 
ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA 
AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT 
TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT 
TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC 
CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA 
ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA 
CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC 
GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG 
CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA 
AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC 
AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA 
GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA 
GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT 
TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( XM_017008312.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863801.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu02420
Clone ID Related Accession (Same CDS sequence)	XM_017008312.2 , XM_005263073.4 , XM_005263073.3 , NM_001371395.1 , XM_017008313.1 , XM_011532037.2 , XM_017008314.1 , XM_017008312.1 , XM_017008315.1 , NM_001371399.1 , NM_019050.2 , XM_017008315.2
Accession Version	XM_017008312.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA 
GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG 
ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA 
ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA 
AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT 
TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT 
TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC 
CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA 
ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA 
CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC 
GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG 
CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA 
AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC 
AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA 
GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA 
GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT 
TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863801.1
CDS	1233..4454
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLKENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEGFRKELRNLEAGYKSHEFHPESHLQIKNHLIKRSHVHEDNGKLFPSSSLQIPKDHNAREHIHQSDEQKLEKPNECKFSEWLNIENSERTGLPFHVDNSASGKRVNSNEPSSLWSSHLRTVGLKPETAPLIQQQNIMDQCYFENSLSTECIIRSASRSDGCQMPKLFCQNLPPPLPPKKYAITSVPQSEKSESTPDVKLTEVFKATSHLPKHSLSTASEPSLEVSTHMNDERHKETFQVRECFGNTPNCPSSSSTNDFQANSGAIDAFCQPELDSISTCPNETVSLTTYFSVDSCMTDTYRLKYHQRPKLSFPESSGFCNNSLS

Target ORF information:

RefSeq Version	XM_017008312.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008312.1

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA 
GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG 
ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA 
ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA 
AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT 
TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT 
TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC 
CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA 
ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA 
CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC 
GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG 
CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA 
AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC 
AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA 
GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA 
GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT 
TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( XM_017008315.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863804.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X5, mRNA.

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CloneID	OHu02420
Clone ID Related Accession (Same CDS sequence)	XM_017008312.2 , XM_005263073.4 , XM_005263073.3 , NM_001371395.1 , XM_017008313.1 , XM_011532037.2 , XM_017008314.1 , XM_017008312.1 , XM_017008315.1 , NM_001371399.1 , NM_019050.2 , XM_017008315.2
Accession Version	XM_017008315.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA 
GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG 
ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA 
ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA 
AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT 
TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT 
TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC 
CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA 
ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA 
CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC 
GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG 
CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA 
AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC 
AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA 
GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA 
GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT 
TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863804.1
CDS	987..4208
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLKENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEGFRKELRNLEAGYKSHEFHPESHLQIKNHLIKRSHVHEDNGKLFPSSSLQIPKDHNAREHIHQSDEQKLEKPNECKFSEWLNIENSERTGLPFHVDNSASGKRVNSNEPSSLWSSHLRTVGLKPETAPLIQQQNIMDQCYFENSLSTECIIRSASRSDGCQMPKLFCQNLPPPLPPKKYAITSVPQSEKSESTPDVKLTEVFKATSHLPKHSLSTASEPSLEVSTHMNDERHKETFQVRECFGNTPNCPSSSSTNDFQANSGAIDAFCQPELDSISTCPNETVSLTTYFSVDSCMTDTYRLKYHQRPKLSFPESSGFCNNSLS

Target ORF information:

RefSeq Version	XM_017008315.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008315.1

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA 
GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG 
ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA 
ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA 
AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT 
TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT 
TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC 
CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA 
ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA 
CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC 
GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG 
CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA 
AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC 
AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA 
GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA 
GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT 
TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( XM_017008315.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863804.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu02420
Clone ID Related Accession (Same CDS sequence)	XM_017008312.2 , XM_005263073.4 , XM_005263073.3 , NM_001371395.1 , XM_017008313.1 , XM_011532037.2 , XM_017008314.1 , XM_017008312.1 , XM_017008315.1 , NM_001371399.1 , NM_019050.2 , XM_017008315.2
Accession Version	XM_017008315.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017008315.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA 
GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG 
ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA 
ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA 
AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT 
TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT 
TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC 
CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA 
ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA 
CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC 
GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG 
CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA 
AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC 
AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA 
GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA 
GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT 
TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863804.1
CDS	1115..4336
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLKENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEGFRKELRNLEAGYKSHEFHPESHLQIKNHLIKRSHVHEDNGKLFPSSSLQIPKDHNAREHIHQSDEQKLEKPNECKFSEWLNIENSERTGLPFHVDNSASGKRVNSNEPSSLWSSHLRTVGLKPETAPLIQQQNIMDQCYFENSLSTECIIRSASRSDGCQMPKLFCQNLPPPLPPKKYAITSVPQSEKSESTPDVKLTEVFKATSHLPKHSLSTASEPSLEVSTHMNDERHKETFQVRECFGNTPNCPSSSSTNDFQANSGAIDAFCQPELDSISTCPNETVSLTTYFSVDSCMTDTYRLKYHQRPKLSFPESSGFCNNSLS

Target ORF information:

RefSeq Version	XM_017008315.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008315.2

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGGATGT 
GAAAAGCCTG TAATTCATAA GTCAGATAAT TTAAAAGAAA ATGGATTTGG TGATCAGGCA 
AAGCAGAGAG AAAATCAGAA ATTTCCAACT GATAATATTT CATCATCTAA TCGGAGCCAC 
AGTCACACAG GTGTAGGGAA AGGACCAGCT AAGTTAAGTC ACATTGATCA AAGGGAAAAG 
ATAAAAGACA TTTCCAGAGA ATGTGCTCTG AAAGCTATTG AACAGAAAAA CTTACTTTCT 
TCACAAAGGA AAGATTTAGA GAAGGGACAA AGAAAAGATT TAGGACGACA TAGAGATTTG 
GTTGATGAAG ACCTTTCACA TTTCCAATCT GGATCACCTC CTGCCCCAAA TGGTTTTAAA 
CAACATGGGA ATCCACATCT ATATCATAGT CAAGGAAAAG GATCATATAA ACATGACCGA 
GTTGTACCTC AGAGTCGAGC TTCTGCACAA ATAATAAGTT CAAGTAAATC CCAGATTCTT 
GCTCCAGGAG AGAAAATAAC TGGCAAAGTT AAGAGTGACA ATGGCACTGG ATATGACACA 
GACAGCAGCC AAGATTCTAG GGATAGAGGA AACAGCTGTG ATAGCAGCAG TAAAAGCCGG 
AACCGAGGTT GGAAACCTAT GAGAGAAACA TTAAATGTTG ATAGTATTTT TAGTGAAAGT 
GAAAAAAGAC AGCATAGTCC AAGACATAAA CCAAATATCA GTAATAAGCC TAAATCTAGC 
AAGGATCCGA GTTTTAGTAA TTGGCCAAAA GAGAATCCAA AGCAAAAAGG TTTAATGACC 
ATATATGAAG ATGAAATGAA GCAGGAAATA GGAAGCAGAA GTTCCCTTGA ATCTAATGGA 
AAAGGAGCAG AGAAAAATAA AGGCCTTGTA GAGGGTAAAG TGCATGGTGA TAATTGGCAG 
ATGCAAAGGA CTGAGTCTGG ATATGAAAGC AGTGATCACA TCAGTAATGG TTCTACTAAT 
TTGGACTCAC CTGTTATCGA TGGAAATGGT ACAGTAATGG ATATCAGTGG TGTTAAAGAA 
ACAGTATGCT TCAGTGACCA GATTACGACA AGCAACCTAA ATAAAGAACG TGGGGACTGT 
ACCTCCCTTC AGAGCCAACA TCACTTAGAA GGCTTTAGAA AAGAACTCAG GAATTTGGAA 
GCAGGCTATA AATCTCATGA ATTCCACCCA GAATCACATT TACAAATAAA AAATCATTTG 
ATAAAAAGAT CACATGTACA TGAAGACAAT GGAAAGTTAT TTCCTTCATC CAGTCTACAA 
ATACCCAAGG ACCATAATGC AAGAGAACAT ATCCACCAGT CAGATGAACA GAAACTTGAA 
AAACCGAATG AATGCAAATT TTCTGAGTGG CTTAATATAG AAAATTCTGA GAGAACAGGT 
TTGCCTTTTC ACGTTGATAA CTCTGCTTCT GGGAAGAGAG TGAACAGTAA TGAACCATCT 
TCATTATGGT CTTCACACCT AAGAACTGTT GGGTTAAAGC CAGAAACTGC TCCTCTCATC 
CAGCAACAAA ATATCATGGA TCAATGTTAC TTTGAGAACT CTCTATCCAC AGAATGTATA 
ATTCGGTCAG CCAGCAGATC TGATGGGTGT CAGATGCCAA AACTTTTTTG CCAGAATCTA 
CCACCCCCTT TGCCACCAAA GAAATATGCT ATAACCAGTG TGCCACAGTC AGAGAAAAGC 
GAATCTACAC CTGATGTCAA ACTTACAGAG GTGTTTAAAG CTACCTCTCA TCTTCCGAAG 
CACAGTTTAA GTACAGCTTC AGAACCAAGT TTAGAAGTGA GTACACATAT GAATGATGAA 
AGACATAAAG AAACATTTCA AGTGAGAGAA TGTTTTGGCA ACACACCAAA CTGTCCATCC 
AGCTCCTCAA CTAACGATTT TCAGGCAAAC TCAGGTGCCA TTGATGCATT TTGCCAACCA 
GAACTAGACT CTATTTCTAC CTGTCCAAAT GAGACAGTTT CATTAACTAC CTATTTTTCA 
GTTGATAGCT GCATGACGGA TACATATAGA TTGAAATACC ATCAGAGGCC CAAGCTCTCT 
TTTCCAGAGA GCAGTGGCTT TTGTAATAAT TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( XM_005263075.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263132.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu38974
Clone ID Related Accession (Same CDS sequence)	XM_005263075.3 , XM_005263075.2
Accession Version	XM_005263075.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005263075.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGATTGGAAC TAGATGGAAA 
GATGTTGTCT CCAAATGCAT TCGATGCCAC TTTCAGCCAC TACTTTTGTT TTATGCAAAC 
CCAGATGGCA CAGCAGTTTC TACTGAGGAT GCACTCAGGC AGGTCATCAG CTGGTCACAT 
TACAAATCTG TTGCAGAAAA TATGGGATGT GAAAAGCCTG TAATTCATAA GTCAGATAAT 
TTAAAAGAAA ATGGATTTGG TGATCAGGCA AAGCAGAGAG AAAATCAGAA ATTTCCAACT 
GATAATATTT CATCATCTAA TCGGAGCCAC AGTCACACAG GTGTAGGGAA AGGACCAGCT 
AAGTTAAGTC ACATTGATCA AAGGGAAAAG ATAAAAGACA TTTCCAGAGA ATGTGCTCTG 
AAAGCTATTG AACAGAAAAA CTTACTTTCT TCACAAAGGA AAGATTTAGA GAAGGGACAA 
AGAAAAGATT TAGGACGACA TAGAGATTTG GTTGATGAAG ACCTTTCACA TTTCCAATCT 
GGATCACCTC CTGCCCCAAA TGGTTTTAAA CAACATGGGA ATCCACATCT ATATCATAGT 
CAAGGAAAAG GATCATATAA ACATGACCGA GTTGTACCTC AGAGTCGAGC TTCTGCACAA 
ATAATAAGTT CAAGTAAATC CCAGATTCTT GCTCCAGGAG AGAAAATAAC TGGCAAAGTT 
AAGAGTGACA ATGGCACTGG ATATGACACA GACAGCAGCC AAGATTCTAG GGATAGAGGA 
AACAGCTGTG ATAGCAGCAG TAAAAGCCGG AACCGAGGTT GGAAACCTAT GAGAGAAACA 
TTAAATGTTG ATAGTATTTT TAGTGAAAGT GAAAAAAGAC AGCATAGTCC AAGACATAAA 
CCAAATATCA GTAATAAGCC TAAATCTAGC AAGGATCCGA GTTTTAGTAA TTGGCCAAAA 
GAGAATCCAA AGCAAAAAGG TTTAATGACC ATATATGAAG ATGAAATGAA GCAGGAAATA 
GGAAGCAGAA GTTCCCTTGA ATCTAATGGA AAAGGAGCAG AGAAAAATAA AGGCCTTGTA 
GAGGGTAAAG TGCATGGTGA TAATTGGCAG ATGCAAAGGA CTGAGTCTGG ATATGAAAGC 
AGTGATCACA TCAGTAATGG TTCTACTAAT TTGGACTCAC CTGTTATCGA TGGAAATGGT 
ACAGTAATGG ATATCAGTGG TGTTAAAGAA ACAGTATGCT TCAGTGACCA GATTACGACA 
AGCAACCTAA ATAAAGAACG TGGGGACTGT ACCTCCCTTC AGAGCCAACA TCACTTAGAA 
GGCTTTAGAA AAGAACTCAG GAATTTGGAA GCAGGCTATA AATCTCATGA ATTCCACCCA 
GAATCACATT TACAAATAAA AAATCATTTG ATAAAAAGAT CACATGTACA TGAAGACAAT 
GGAAAGTTAT TTCCTTCATC CAGTCTACAA ATACCCAAGG ACCATAATGC AAGAGAACAT 
ATCCACCAGT CAGATGAACA GAAACTTGAA AAACCGAATG AATGCAAATT TTCTGAGTGG 
CTTAATATAG AAAATTCTGA GAGAACAGGT TTGCCTTTTC ACGTTGATAA CTCTGCTTCT 
GGGAAGAGAG TGAACAGTAA TGAACCATCT TCATTATGGT CTTCACACCT AAGAACTGTT 
GGGTTAAAGC CAGAAACTGC TCCTCTCATC CAGCAACAAA ATATCATGGA TCAATGTTAC 
TTTGAGAACT CTCTATCCAC AGAATGTATA ATTCGGTCAG CCAGCAGATC TGATGGGTGT 
CAGATGCCAA AACTTTTTTG CCAGAATCTA CCACCCCCTT TGCCACCAAA GAAATATGCT 
ATAACCAGTG TGCCACAGTC AGAGAAAAGC GAATCTACAC CTGATGTCAA ACTTACAGAG 
GTGTTTAAAG CTACCTCTCA TCTTCCGAAG CACAGTTTAA GTACAGCTTC AGAACCAAGT 
TTAGAAGTGA GTACACATAT GAATGATGAA AGACATAAAG AAACATTTCA AGTGAGAGAA 
TGTTTTGGCA ACACACCAAA CTGTCCATCC AGCTCCTCAA CTAACGATTT TCAGGCAAAC 
TCAGGTGCCA TTGATGCATT TTGCCAACCA GAACTAGACT CTATTTCTAC CTGTCCAAAT 
GAGACAGTTT CATTAACTAC CTATTTTTCA GTTGATAGCT GCATGACGGA TACATATAGA 
TTGAAATACC ATCAGAGGCC CAAGCTCTCT TTTCCAGAGA GCAGTGGCTT TTGTAATAAT 
TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263132.1
CDS	1067..4138
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLKENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEGFRKELRNLEAGYKSHEFHPESHLQIKNHLIKRSHVHEDNGKLFPSSSLQIPKDHNAREHIHQSDEQKLEKPNECKFSEWLNIENSERTGLPFHVDNSASGKRVNSNEPSSLWSSHLRTVGLKPETAPLIQQQNIMDQCYFENSLSTECIIRSASRSDGCQMPKLFCQNLPPPLPPKKYAITSVPQSEKSESTPDVKLTEVFKATSHLPKHSLSTASEPSLEVSTHMNDERHKETFQVRECFGNTPNCPSSSSTNDFQANSGAIDAFCQPELDSISTCPNETVSLTTYFSVDSCMTDTYRLKYHQRPKLSFPESSGFCNNSLS

Target ORF information:

RefSeq Version	XM_005263075.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263075.3

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGATTGGAAC TAGATGGAAA 
GATGTTGTCT CCAAATGCAT TCGATGCCAC TTTCAGCCAC TACTTTTGTT TTATGCAAAC 
CCAGATGGCA CAGCAGTTTC TACTGAGGAT GCACTCAGGC AGGTCATCAG CTGGTCACAT 
TACAAATCTG TTGCAGAAAA TATGGGATGT GAAAAGCCTG TAATTCATAA GTCAGATAAT 
TTAAAAGAAA ATGGATTTGG TGATCAGGCA AAGCAGAGAG AAAATCAGAA ATTTCCAACT 
GATAATATTT CATCATCTAA TCGGAGCCAC AGTCACACAG GTGTAGGGAA AGGACCAGCT 
AAGTTAAGTC ACATTGATCA AAGGGAAAAG ATAAAAGACA TTTCCAGAGA ATGTGCTCTG 
AAAGCTATTG AACAGAAAAA CTTACTTTCT TCACAAAGGA AAGATTTAGA GAAGGGACAA 
AGAAAAGATT TAGGACGACA TAGAGATTTG GTTGATGAAG ACCTTTCACA TTTCCAATCT 
GGATCACCTC CTGCCCCAAA TGGTTTTAAA CAACATGGGA ATCCACATCT ATATCATAGT 
CAAGGAAAAG GATCATATAA ACATGACCGA GTTGTACCTC AGAGTCGAGC TTCTGCACAA 
ATAATAAGTT CAAGTAAATC CCAGATTCTT GCTCCAGGAG AGAAAATAAC TGGCAAAGTT 
AAGAGTGACA ATGGCACTGG ATATGACACA GACAGCAGCC AAGATTCTAG GGATAGAGGA 
AACAGCTGTG ATAGCAGCAG TAAAAGCCGG AACCGAGGTT GGAAACCTAT GAGAGAAACA 
TTAAATGTTG ATAGTATTTT TAGTGAAAGT GAAAAAAGAC AGCATAGTCC AAGACATAAA 
CCAAATATCA GTAATAAGCC TAAATCTAGC AAGGATCCGA GTTTTAGTAA TTGGCCAAAA 
GAGAATCCAA AGCAAAAAGG TTTAATGACC ATATATGAAG ATGAAATGAA GCAGGAAATA 
GGAAGCAGAA GTTCCCTTGA ATCTAATGGA AAAGGAGCAG AGAAAAATAA AGGCCTTGTA 
GAGGGTAAAG TGCATGGTGA TAATTGGCAG ATGCAAAGGA CTGAGTCTGG ATATGAAAGC 
AGTGATCACA TCAGTAATGG TTCTACTAAT TTGGACTCAC CTGTTATCGA TGGAAATGGT 
ACAGTAATGG ATATCAGTGG TGTTAAAGAA ACAGTATGCT TCAGTGACCA GATTACGACA 
AGCAACCTAA ATAAAGAACG TGGGGACTGT ACCTCCCTTC AGAGCCAACA TCACTTAGAA 
GGCTTTAGAA AAGAACTCAG GAATTTGGAA GCAGGCTATA AATCTCATGA ATTCCACCCA 
GAATCACATT TACAAATAAA AAATCATTTG ATAAAAAGAT CACATGTACA TGAAGACAAT 
GGAAAGTTAT TTCCTTCATC CAGTCTACAA ATACCCAAGG ACCATAATGC AAGAGAACAT 
ATCCACCAGT CAGATGAACA GAAACTTGAA AAACCGAATG AATGCAAATT TTCTGAGTGG 
CTTAATATAG AAAATTCTGA GAGAACAGGT TTGCCTTTTC ACGTTGATAA CTCTGCTTCT 
GGGAAGAGAG TGAACAGTAA TGAACCATCT TCATTATGGT CTTCACACCT AAGAACTGTT 
GGGTTAAAGC CAGAAACTGC TCCTCTCATC CAGCAACAAA ATATCATGGA TCAATGTTAC 
TTTGAGAACT CTCTATCCAC AGAATGTATA ATTCGGTCAG CCAGCAGATC TGATGGGTGT 
CAGATGCCAA AACTTTTTTG CCAGAATCTA CCACCCCCTT TGCCACCAAA GAAATATGCT 
ATAACCAGTG TGCCACAGTC AGAGAAAAGC GAATCTACAC CTGATGTCAA ACTTACAGAG 
GTGTTTAAAG CTACCTCTCA TCTTCCGAAG CACAGTTTAA GTACAGCTTC AGAACCAAGT 
TTAGAAGTGA GTACACATAT GAATGATGAA AGACATAAAG AAACATTTCA AGTGAGAGAA 
TGTTTTGGCA ACACACCAAA CTGTCCATCC AGCTCCTCAA CTAACGATTT TCAGGCAAAC 
TCAGGTGCCA TTGATGCATT TTGCCAACCA GAACTAGACT CTATTTCTAC CTGTCCAAAT 
GAGACAGTTT CATTAACTAC CTATTTTTCA GTTGATAGCT GCATGACGGA TACATATAGA 
TTGAAATACC ATCAGAGGCC CAAGCTCTCT TTTCCAGAGA GCAGTGGCTT TTGTAATAAT 
TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( XM_005263075.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263132.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu38974
Clone ID Related Accession (Same CDS sequence)	XM_005263075.3 , XM_005263075.2
Accession Version	XM_005263075.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263075.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGATTGGAAC TAGATGGAAA 
GATGTTGTCT CCAAATGCAT TCGATGCCAC TTTCAGCCAC TACTTTTGTT TTATGCAAAC 
CCAGATGGCA CAGCAGTTTC TACTGAGGAT GCACTCAGGC AGGTCATCAG CTGGTCACAT 
TACAAATCTG TTGCAGAAAA TATGGGATGT GAAAAGCCTG TAATTCATAA GTCAGATAAT 
TTAAAAGAAA ATGGATTTGG TGATCAGGCA AAGCAGAGAG AAAATCAGAA ATTTCCAACT 
GATAATATTT CATCATCTAA TCGGAGCCAC AGTCACACAG GTGTAGGGAA AGGACCAGCT 
AAGTTAAGTC ACATTGATCA AAGGGAAAAG ATAAAAGACA TTTCCAGAGA ATGTGCTCTG 
AAAGCTATTG AACAGAAAAA CTTACTTTCT TCACAAAGGA AAGATTTAGA GAAGGGACAA 
AGAAAAGATT TAGGACGACA TAGAGATTTG GTTGATGAAG ACCTTTCACA TTTCCAATCT 
GGATCACCTC CTGCCCCAAA TGGTTTTAAA CAACATGGGA ATCCACATCT ATATCATAGT 
CAAGGAAAAG GATCATATAA ACATGACCGA GTTGTACCTC AGAGTCGAGC TTCTGCACAA 
ATAATAAGTT CAAGTAAATC CCAGATTCTT GCTCCAGGAG AGAAAATAAC TGGCAAAGTT 
AAGAGTGACA ATGGCACTGG ATATGACACA GACAGCAGCC AAGATTCTAG GGATAGAGGA 
AACAGCTGTG ATAGCAGCAG TAAAAGCCGG AACCGAGGTT GGAAACCTAT GAGAGAAACA 
TTAAATGTTG ATAGTATTTT TAGTGAAAGT GAAAAAAGAC AGCATAGTCC AAGACATAAA 
CCAAATATCA GTAATAAGCC TAAATCTAGC AAGGATCCGA GTTTTAGTAA TTGGCCAAAA 
GAGAATCCAA AGCAAAAAGG TTTAATGACC ATATATGAAG ATGAAATGAA GCAGGAAATA 
GGAAGCAGAA GTTCCCTTGA ATCTAATGGA AAAGGAGCAG AGAAAAATAA AGGCCTTGTA 
GAGGGTAAAG TGCATGGTGA TAATTGGCAG ATGCAAAGGA CTGAGTCTGG ATATGAAAGC 
AGTGATCACA TCAGTAATGG TTCTACTAAT TTGGACTCAC CTGTTATCGA TGGAAATGGT 
ACAGTAATGG ATATCAGTGG TGTTAAAGAA ACAGTATGCT TCAGTGACCA GATTACGACA 
AGCAACCTAA ATAAAGAACG TGGGGACTGT ACCTCCCTTC AGAGCCAACA TCACTTAGAA 
GGCTTTAGAA AAGAACTCAG GAATTTGGAA GCAGGCTATA AATCTCATGA ATTCCACCCA 
GAATCACATT TACAAATAAA AAATCATTTG ATAAAAAGAT CACATGTACA TGAAGACAAT 
GGAAAGTTAT TTCCTTCATC CAGTCTACAA ATACCCAAGG ACCATAATGC AAGAGAACAT 
ATCCACCAGT CAGATGAACA GAAACTTGAA AAACCGAATG AATGCAAATT TTCTGAGTGG 
CTTAATATAG AAAATTCTGA GAGAACAGGT TTGCCTTTTC ACGTTGATAA CTCTGCTTCT 
GGGAAGAGAG TGAACAGTAA TGAACCATCT TCATTATGGT CTTCACACCT AAGAACTGTT 
GGGTTAAAGC CAGAAACTGC TCCTCTCATC CAGCAACAAA ATATCATGGA TCAATGTTAC 
TTTGAGAACT CTCTATCCAC AGAATGTATA ATTCGGTCAG CCAGCAGATC TGATGGGTGT 
CAGATGCCAA AACTTTTTTG CCAGAATCTA CCACCCCCTT TGCCACCAAA GAAATATGCT 
ATAACCAGTG TGCCACAGTC AGAGAAAAGC GAATCTACAC CTGATGTCAA ACTTACAGAG 
GTGTTTAAAG CTACCTCTCA TCTTCCGAAG CACAGTTTAA GTACAGCTTC AGAACCAAGT 
TTAGAAGTGA GTACACATAT GAATGATGAA AGACATAAAG AAACATTTCA AGTGAGAGAA 
TGTTTTGGCA ACACACCAAA CTGTCCATCC AGCTCCTCAA CTAACGATTT TCAGGCAAAC 
TCAGGTGCCA TTGATGCATT TTGCCAACCA GAACTAGACT CTATTTCTAC CTGTCCAAAT 
GAGACAGTTT CATTAACTAC CTATTTTTCA GTTGATAGCT GCATGACGGA TACATATAGA 
TTGAAATACC ATCAGAGGCC CAAGCTCTCT TTTCCAGAGA GCAGTGGCTT TTGTAATAAT 
TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263132.1
CDS	1106..4177
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLKENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEGFRKELRNLEAGYKSHEFHPESHLQIKNHLIKRSHVHEDNGKLFPSSSLQIPKDHNAREHIHQSDEQKLEKPNECKFSEWLNIENSERTGLPFHVDNSASGKRVNSNEPSSLWSSHLRTVGLKPETAPLIQQQNIMDQCYFENSLSTECIIRSASRSDGCQMPKLFCQNLPPPLPPKKYAITSVPQSEKSESTPDVKLTEVFKATSHLPKHSLSTASEPSLEVSTHMNDERHKETFQVRECFGNTPNCPSSSSTNDFQANSGAIDAFCQPELDSISTCPNETVSLTTYFSVDSCMTDTYRLKYHQRPKLSFPESSGFCNNSLS

Target ORF information:

RefSeq Version	XM_005263075.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263075.2

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGATTGGAAC TAGATGGAAA 
GATGTTGTCT CCAAATGCAT TCGATGCCAC TTTCAGCCAC TACTTTTGTT TTATGCAAAC 
CCAGATGGCA CAGCAGTTTC TACTGAGGAT GCACTCAGGC AGGTCATCAG CTGGTCACAT 
TACAAATCTG TTGCAGAAAA TATGGGATGT GAAAAGCCTG TAATTCATAA GTCAGATAAT 
TTAAAAGAAA ATGGATTTGG TGATCAGGCA AAGCAGAGAG AAAATCAGAA ATTTCCAACT 
GATAATATTT CATCATCTAA TCGGAGCCAC AGTCACACAG GTGTAGGGAA AGGACCAGCT 
AAGTTAAGTC ACATTGATCA AAGGGAAAAG ATAAAAGACA TTTCCAGAGA ATGTGCTCTG 
AAAGCTATTG AACAGAAAAA CTTACTTTCT TCACAAAGGA AAGATTTAGA GAAGGGACAA 
AGAAAAGATT TAGGACGACA TAGAGATTTG GTTGATGAAG ACCTTTCACA TTTCCAATCT 
GGATCACCTC CTGCCCCAAA TGGTTTTAAA CAACATGGGA ATCCACATCT ATATCATAGT 
CAAGGAAAAG GATCATATAA ACATGACCGA GTTGTACCTC AGAGTCGAGC TTCTGCACAA 
ATAATAAGTT CAAGTAAATC CCAGATTCTT GCTCCAGGAG AGAAAATAAC TGGCAAAGTT 
AAGAGTGACA ATGGCACTGG ATATGACACA GACAGCAGCC AAGATTCTAG GGATAGAGGA 
AACAGCTGTG ATAGCAGCAG TAAAAGCCGG AACCGAGGTT GGAAACCTAT GAGAGAAACA 
TTAAATGTTG ATAGTATTTT TAGTGAAAGT GAAAAAAGAC AGCATAGTCC AAGACATAAA 
CCAAATATCA GTAATAAGCC TAAATCTAGC AAGGATCCGA GTTTTAGTAA TTGGCCAAAA 
GAGAATCCAA AGCAAAAAGG TTTAATGACC ATATATGAAG ATGAAATGAA GCAGGAAATA 
GGAAGCAGAA GTTCCCTTGA ATCTAATGGA AAAGGAGCAG AGAAAAATAA AGGCCTTGTA 
GAGGGTAAAG TGCATGGTGA TAATTGGCAG ATGCAAAGGA CTGAGTCTGG ATATGAAAGC 
AGTGATCACA TCAGTAATGG TTCTACTAAT TTGGACTCAC CTGTTATCGA TGGAAATGGT 
ACAGTAATGG ATATCAGTGG TGTTAAAGAA ACAGTATGCT TCAGTGACCA GATTACGACA 
AGCAACCTAA ATAAAGAACG TGGGGACTGT ACCTCCCTTC AGAGCCAACA TCACTTAGAA 
GGCTTTAGAA AAGAACTCAG GAATTTGGAA GCAGGCTATA AATCTCATGA ATTCCACCCA 
GAATCACATT TACAAATAAA AAATCATTTG ATAAAAAGAT CACATGTACA TGAAGACAAT 
GGAAAGTTAT TTCCTTCATC CAGTCTACAA ATACCCAAGG ACCATAATGC AAGAGAACAT 
ATCCACCAGT CAGATGAACA GAAACTTGAA AAACCGAATG AATGCAAATT TTCTGAGTGG 
CTTAATATAG AAAATTCTGA GAGAACAGGT TTGCCTTTTC ACGTTGATAA CTCTGCTTCT 
GGGAAGAGAG TGAACAGTAA TGAACCATCT TCATTATGGT CTTCACACCT AAGAACTGTT 
GGGTTAAAGC CAGAAACTGC TCCTCTCATC CAGCAACAAA ATATCATGGA TCAATGTTAC 
TTTGAGAACT CTCTATCCAC AGAATGTATA ATTCGGTCAG CCAGCAGATC TGATGGGTGT 
CAGATGCCAA AACTTTTTTG CCAGAATCTA CCACCCCCTT TGCCACCAAA GAAATATGCT 
ATAACCAGTG TGCCACAGTC AGAGAAAAGC GAATCTACAC CTGATGTCAA ACTTACAGAG 
GTGTTTAAAG CTACCTCTCA TCTTCCGAAG CACAGTTTAA GTACAGCTTC AGAACCAAGT 
TTAGAAGTGA GTACACATAT GAATGATGAA AGACATAAAG AAACATTTCA AGTGAGAGAA 
TGTTTTGGCA ACACACCAAA CTGTCCATCC AGCTCCTCAA CTAACGATTT TCAGGCAAAC 
TCAGGTGCCA TTGATGCATT TTGCCAACCA GAACTAGACT CTATTTCTAC CTGTCCAAAT 
GAGACAGTTT CATTAACTAC CTATTTTTCA GTTGATAGCT GCATGACGGA TACATATAGA 
TTGAAATACC ATCAGAGGCC CAAGCTCTCT TTTCCAGAGA GCAGTGGCTT TTGTAATAAT 
TCACTATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( XM_005263076.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263133.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu38975
Clone ID Related Accession (Same CDS sequence)	NM_001371396.1 , XM_005263076.2 , XM_017008316.1 , XM_017008316.2 , XM_005263076.3
Accession Version	XM_005263076.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263076.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGCTAAG 
TTAAGTCACA TTGATCAAAG GGAAAAGATA AAAGACATTT CCAGAGAATG TGCTCTGAAA 
GCTATTGAAC AGAAAAACTT ACTTTCTTCA CAAAGGAAAG ATTTAGAGAA GGGACAAAGA 
AAAGATTTAG GACGACATAG AGATTTGGTT GATGAAGACC TTTCACATTT CCAATCTGGA 
TCACCTCCTG CCCCAAATGG TTTTAAACAA CATGGGAATC CACATCTATA TCATAGTCAA 
GGAAAAGGAT CATATAAACA TGACCGAGTT GTACCTCAGA GTCGAGCTTC TGCACAAATA 
ATAAGTTCAA GTAAATCCCA GATTCTTGCT CCAGGAGAGA AAATAACTGG CAAAGTTAAG 
AGTGACAATG GCACTGGATA TGACACAGAC AGCAGCCAAG ATTCTAGGGA TAGAGGAAAC 
AGCTGTGATA GCAGCAGTAA AAGCCGGAAC CGAGGTTGGA AACCTATGAG AGAAACATTA 
AATGTTGATA GTATTTTTAG TGAAAGTGAA AAAAGACAGC ATAGTCCAAG ACATAAACCA 
AATATCAGTA ATAAGCCTAA ATCTAGCAAG GATCCGAGTT TTAGTAATTG GCCAAAAGAG 
AATCCAAAGC AAAAAGGTTT AATGACCATA TATGAAGATG AAATGAAGCA GGAAATAGGA 
AGCAGAAGTT CCCTTGAATC TAATGGAAAA GGAGCAGAGA AAAATAAAGG CCTTGTAGAG 
GGTAAAGTGC ATGGTGATAA TTGGCAGATG CAAAGGACTG AGTCTGGATA TGAAAGCAGT 
GATCACATCA GTAATGGTTC TACTAATTTG GACTCACCTG TTATCGATGG AAATGGTACA 
GTAATGGATA TCAGTGGTGT TAAAGAAACA GTATGCTTCA GTGACCAGAT TACGACAAGC 
AACCTAAATA AAGAACGTGG GGACTGTACC TCCCTTCAGA GCCAACATCA CTTAGAAGGC 
TTTAGAAAAG AACTCAGGAA TTTGGAAGCA GGCTATAAAT CTCATGAATT CCACCCAGAA 
TCACATTTAC AAATAAAAAA TCATTTGATA AAAAGATCAC ATGTACATGA AGACAATGGA 
AAGTTATTTC CTTCATCCAG TCTACAAATA CCCAAGGACC ATAATGCAAG AGAACATATC 
CACCAGTCAG ATGAACAGAA ACTTGAAAAA CCGAATGAAT GCAAATTTTC TGAGTGGCTT 
AATATAGAAA ATTCTGAGAG AACAGGTTTG CCTTTTCACG TTGATAACTC TGCTTCTGGG 
AAGAGAGTGA ACAGTAATGA ACCATCTTCA TTATGGTCTT CACACCTAAG AACTGTTGGG 
TTAAAGCCAG AAACTGCTCC TCTCATCCAG CAACAAAATA TCATGGATCA ATGTTACTTT 
GAGAACTCTC TATCCACAGA ATGTATAATT CGGTCAGCCA GCAGATCTGA TGGGTGTCAG 
ATGCCAAAAC TTTTTTGCCA GAATCTACCA CCCCCTTTGC CACCAAAGAA ATATGCTATA 
ACCAGTGTGC CACAGTCAGA GAAAAGCGAA TCTACACCTG ATGTCAAACT TACAGAGGTG 
TTTAAAGCTA CCTCTCATCT TCCGAAGCAC AGTTTAAGTA CAGCTTCAGA ACCAAGTTTA 
GAAGTGAGTA CACATATGAA TGATGAAAGA CATAAAGAAA CATTTCAAGT GAGAGAATGT 
TTTGGCAACA CACCAAACTG TCCATCCAGC TCCTCAACTA ACGATTTTCA GGCAAACTCA 
GGTGCCATTG ATGCATTTTG CCAACCAGAA CTAGACTCTA TTTCTACCTG TCCAAATGAG 
ACAGTTTCAT TAACTACCTA TTTTTCAGTT GATAGCTGCA TGACGGATAC ATATAGATTG 
AAATACCATC AGAGGCCCAA GCTCTCTTTT CCAGAGAGCA GTGGCTTTTG TAATAATTCA 
CTATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263133.1
CDS	1106..4174
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEGFRKELRNLEAGYKSHEFHPESHLQIKNHLIKRSHVHEDNGKLFPSSSLQIPKDHNAREHIHQSDEQKLEKPNECKFSEWLNIENSERTGLPFHVDNSASGKRVNSNEPSSLWSSHLRTVGLKPETAPLIQQQNIMDQCYFENSLSTECIIRSASRSDGCQMPKLFCQNLPPPLPPKKYAITSVPQSEKSESTPDVKLTEVFKATSHLPKHSLSTASEPSLEVSTHMNDERHKETFQVRECFGNTPNCPSSSSTNDFQANSGAIDAFCQPELDSISTCPNETVSLTTYFSVDSCMTDTYRLKYHQRPKLSFPESSGFCNNSLS

Target ORF information:

RefSeq Version	XM_005263076.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263076.2

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGCTAAG 
TTAAGTCACA TTGATCAAAG GGAAAAGATA AAAGACATTT CCAGAGAATG TGCTCTGAAA 
GCTATTGAAC AGAAAAACTT ACTTTCTTCA CAAAGGAAAG ATTTAGAGAA GGGACAAAGA 
AAAGATTTAG GACGACATAG AGATTTGGTT GATGAAGACC TTTCACATTT CCAATCTGGA 
TCACCTCCTG CCCCAAATGG TTTTAAACAA CATGGGAATC CACATCTATA TCATAGTCAA 
GGAAAAGGAT CATATAAACA TGACCGAGTT GTACCTCAGA GTCGAGCTTC TGCACAAATA 
ATAAGTTCAA GTAAATCCCA GATTCTTGCT CCAGGAGAGA AAATAACTGG CAAAGTTAAG 
AGTGACAATG GCACTGGATA TGACACAGAC AGCAGCCAAG ATTCTAGGGA TAGAGGAAAC 
AGCTGTGATA GCAGCAGTAA AAGCCGGAAC CGAGGTTGGA AACCTATGAG AGAAACATTA 
AATGTTGATA GTATTTTTAG TGAAAGTGAA AAAAGACAGC ATAGTCCAAG ACATAAACCA 
AATATCAGTA ATAAGCCTAA ATCTAGCAAG GATCCGAGTT TTAGTAATTG GCCAAAAGAG 
AATCCAAAGC AAAAAGGTTT AATGACCATA TATGAAGATG AAATGAAGCA GGAAATAGGA 
AGCAGAAGTT CCCTTGAATC TAATGGAAAA GGAGCAGAGA AAAATAAAGG CCTTGTAGAG 
GGTAAAGTGC ATGGTGATAA TTGGCAGATG CAAAGGACTG AGTCTGGATA TGAAAGCAGT 
GATCACATCA GTAATGGTTC TACTAATTTG GACTCACCTG TTATCGATGG AAATGGTACA 
GTAATGGATA TCAGTGGTGT TAAAGAAACA GTATGCTTCA GTGACCAGAT TACGACAAGC 
AACCTAAATA AAGAACGTGG GGACTGTACC TCCCTTCAGA GCCAACATCA CTTAGAAGGC 
TTTAGAAAAG AACTCAGGAA TTTGGAAGCA GGCTATAAAT CTCATGAATT CCACCCAGAA 
TCACATTTAC AAATAAAAAA TCATTTGATA AAAAGATCAC ATGTACATGA AGACAATGGA 
AAGTTATTTC CTTCATCCAG TCTACAAATA CCCAAGGACC ATAATGCAAG AGAACATATC 
CACCAGTCAG ATGAACAGAA ACTTGAAAAA CCGAATGAAT GCAAATTTTC TGAGTGGCTT 
AATATAGAAA ATTCTGAGAG AACAGGTTTG CCTTTTCACG TTGATAACTC TGCTTCTGGG 
AAGAGAGTGA ACAGTAATGA ACCATCTTCA TTATGGTCTT CACACCTAAG AACTGTTGGG 
TTAAAGCCAG AAACTGCTCC TCTCATCCAG CAACAAAATA TCATGGATCA ATGTTACTTT 
GAGAACTCTC TATCCACAGA ATGTATAATT CGGTCAGCCA GCAGATCTGA TGGGTGTCAG 
ATGCCAAAAC TTTTTTGCCA GAATCTACCA CCCCCTTTGC CACCAAAGAA ATATGCTATA 
ACCAGTGTGC CACAGTCAGA GAAAAGCGAA TCTACACCTG ATGTCAAACT TACAGAGGTG 
TTTAAAGCTA CCTCTCATCT TCCGAAGCAC AGTTTAAGTA CAGCTTCAGA ACCAAGTTTA 
GAAGTGAGTA CACATATGAA TGATGAAAGA CATAAAGAAA CATTTCAAGT GAGAGAATGT 
TTTGGCAACA CACCAAACTG TCCATCCAGC TCCTCAACTA ACGATTTTCA GGCAAACTCA 
GGTGCCATTG ATGCATTTTG CCAACCAGAA CTAGACTCTA TTTCTACCTG TCCAAATGAG 
ACAGTTTCAT TAACTACCTA TTTTTCAGTT GATAGCTGCA TGACGGATAC ATATAGATTG 
AAATACCATC AGAGGCCCAA GCTCTCTTTT CCAGAGAGCA GTGGCTTTTG TAATAATTCA 
CTATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( XM_017008316.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863805.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu38975
Clone ID Related Accession (Same CDS sequence)	NM_001371396.1 , XM_005263076.2 , XM_017008316.1 , XM_017008316.2 , XM_005263076.3
Accession Version	XM_017008316.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGCTAAG 
TTAAGTCACA TTGATCAAAG GGAAAAGATA AAAGACATTT CCAGAGAATG TGCTCTGAAA 
GCTATTGAAC AGAAAAACTT ACTTTCTTCA CAAAGGAAAG ATTTAGAGAA GGGACAAAGA 
AAAGATTTAG GACGACATAG AGATTTGGTT GATGAAGACC TTTCACATTT CCAATCTGGA 
TCACCTCCTG CCCCAAATGG TTTTAAACAA CATGGGAATC CACATCTATA TCATAGTCAA 
GGAAAAGGAT CATATAAACA TGACCGAGTT GTACCTCAGA GTCGAGCTTC TGCACAAATA 
ATAAGTTCAA GTAAATCCCA GATTCTTGCT CCAGGAGAGA AAATAACTGG CAAAGTTAAG 
AGTGACAATG GCACTGGATA TGACACAGAC AGCAGCCAAG ATTCTAGGGA TAGAGGAAAC 
AGCTGTGATA GCAGCAGTAA AAGCCGGAAC CGAGGTTGGA AACCTATGAG AGAAACATTA 
AATGTTGATA GTATTTTTAG TGAAAGTGAA AAAAGACAGC ATAGTCCAAG ACATAAACCA 
AATATCAGTA ATAAGCCTAA ATCTAGCAAG GATCCGAGTT TTAGTAATTG GCCAAAAGAG 
AATCCAAAGC AAAAAGGTTT AATGACCATA TATGAAGATG AAATGAAGCA GGAAATAGGA 
AGCAGAAGTT CCCTTGAATC TAATGGAAAA GGAGCAGAGA AAAATAAAGG CCTTGTAGAG 
GGTAAAGTGC ATGGTGATAA TTGGCAGATG CAAAGGACTG AGTCTGGATA TGAAAGCAGT 
GATCACATCA GTAATGGTTC TACTAATTTG GACTCACCTG TTATCGATGG AAATGGTACA 
GTAATGGATA TCAGTGGTGT TAAAGAAACA GTATGCTTCA GTGACCAGAT TACGACAAGC 
AACCTAAATA AAGAACGTGG GGACTGTACC TCCCTTCAGA GCCAACATCA CTTAGAAGGC 
TTTAGAAAAG AACTCAGGAA TTTGGAAGCA GGCTATAAAT CTCATGAATT CCACCCAGAA 
TCACATTTAC AAATAAAAAA TCATTTGATA AAAAGATCAC ATGTACATGA AGACAATGGA 
AAGTTATTTC CTTCATCCAG TCTACAAATA CCCAAGGACC ATAATGCAAG AGAACATATC 
CACCAGTCAG ATGAACAGAA ACTTGAAAAA CCGAATGAAT GCAAATTTTC TGAGTGGCTT 
AATATAGAAA ATTCTGAGAG AACAGGTTTG CCTTTTCACG TTGATAACTC TGCTTCTGGG 
AAGAGAGTGA ACAGTAATGA ACCATCTTCA TTATGGTCTT CACACCTAAG AACTGTTGGG 
TTAAAGCCAG AAACTGCTCC TCTCATCCAG CAACAAAATA TCATGGATCA ATGTTACTTT 
GAGAACTCTC TATCCACAGA ATGTATAATT CGGTCAGCCA GCAGATCTGA TGGGTGTCAG 
ATGCCAAAAC TTTTTTGCCA GAATCTACCA CCCCCTTTGC CACCAAAGAA ATATGCTATA 
ACCAGTGTGC CACAGTCAGA GAAAAGCGAA TCTACACCTG ATGTCAAACT TACAGAGGTG 
TTTAAAGCTA CCTCTCATCT TCCGAAGCAC AGTTTAAGTA CAGCTTCAGA ACCAAGTTTA 
GAAGTGAGTA CACATATGAA TGATGAAAGA CATAAAGAAA CATTTCAAGT GAGAGAATGT 
TTTGGCAACA CACCAAACTG TCCATCCAGC TCCTCAACTA ACGATTTTCA GGCAAACTCA 
GGTGCCATTG ATGCATTTTG CCAACCAGAA CTAGACTCTA TTTCTACCTG TCCAAATGAG 
ACAGTTTCAT TAACTACCTA TTTTTCAGTT GATAGCTGCA TGACGGATAC ATATAGATTG 
AAATACCATC AGAGGCCCAA GCTCTCTTTT CCAGAGAGCA GTGGCTTTTG TAATAATTCA 
CTATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863805.1
CDS	958..4026
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEGFRKELRNLEAGYKSHEFHPESHLQIKNHLIKRSHVHEDNGKLFPSSSLQIPKDHNAREHIHQSDEQKLEKPNECKFSEWLNIENSERTGLPFHVDNSASGKRVNSNEPSSLWSSHLRTVGLKPETAPLIQQQNIMDQCYFENSLSTECIIRSASRSDGCQMPKLFCQNLPPPLPPKKYAITSVPQSEKSESTPDVKLTEVFKATSHLPKHSLSTASEPSLEVSTHMNDERHKETFQVRECFGNTPNCPSSSSTNDFQANSGAIDAFCQPELDSISTCPNETVSLTTYFSVDSCMTDTYRLKYHQRPKLSFPESSGFCNNSLS

Target ORF information:

RefSeq Version	XM_017008316.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008316.1

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGCTAAG 
TTAAGTCACA TTGATCAAAG GGAAAAGATA AAAGACATTT CCAGAGAATG TGCTCTGAAA 
GCTATTGAAC AGAAAAACTT ACTTTCTTCA CAAAGGAAAG ATTTAGAGAA GGGACAAAGA 
AAAGATTTAG GACGACATAG AGATTTGGTT GATGAAGACC TTTCACATTT CCAATCTGGA 
TCACCTCCTG CCCCAAATGG TTTTAAACAA CATGGGAATC CACATCTATA TCATAGTCAA 
GGAAAAGGAT CATATAAACA TGACCGAGTT GTACCTCAGA GTCGAGCTTC TGCACAAATA 
ATAAGTTCAA GTAAATCCCA GATTCTTGCT CCAGGAGAGA AAATAACTGG CAAAGTTAAG 
AGTGACAATG GCACTGGATA TGACACAGAC AGCAGCCAAG ATTCTAGGGA TAGAGGAAAC 
AGCTGTGATA GCAGCAGTAA AAGCCGGAAC CGAGGTTGGA AACCTATGAG AGAAACATTA 
AATGTTGATA GTATTTTTAG TGAAAGTGAA AAAAGACAGC ATAGTCCAAG ACATAAACCA 
AATATCAGTA ATAAGCCTAA ATCTAGCAAG GATCCGAGTT TTAGTAATTG GCCAAAAGAG 
AATCCAAAGC AAAAAGGTTT AATGACCATA TATGAAGATG AAATGAAGCA GGAAATAGGA 
AGCAGAAGTT CCCTTGAATC TAATGGAAAA GGAGCAGAGA AAAATAAAGG CCTTGTAGAG 
GGTAAAGTGC ATGGTGATAA TTGGCAGATG CAAAGGACTG AGTCTGGATA TGAAAGCAGT 
GATCACATCA GTAATGGTTC TACTAATTTG GACTCACCTG TTATCGATGG AAATGGTACA 
GTAATGGATA TCAGTGGTGT TAAAGAAACA GTATGCTTCA GTGACCAGAT TACGACAAGC 
AACCTAAATA AAGAACGTGG GGACTGTACC TCCCTTCAGA GCCAACATCA CTTAGAAGGC 
TTTAGAAAAG AACTCAGGAA TTTGGAAGCA GGCTATAAAT CTCATGAATT CCACCCAGAA 
TCACATTTAC AAATAAAAAA TCATTTGATA AAAAGATCAC ATGTACATGA AGACAATGGA 
AAGTTATTTC CTTCATCCAG TCTACAAATA CCCAAGGACC ATAATGCAAG AGAACATATC 
CACCAGTCAG ATGAACAGAA ACTTGAAAAA CCGAATGAAT GCAAATTTTC TGAGTGGCTT 
AATATAGAAA ATTCTGAGAG AACAGGTTTG CCTTTTCACG TTGATAACTC TGCTTCTGGG 
AAGAGAGTGA ACAGTAATGA ACCATCTTCA TTATGGTCTT CACACCTAAG AACTGTTGGG 
TTAAAGCCAG AAACTGCTCC TCTCATCCAG CAACAAAATA TCATGGATCA ATGTTACTTT 
GAGAACTCTC TATCCACAGA ATGTATAATT CGGTCAGCCA GCAGATCTGA TGGGTGTCAG 
ATGCCAAAAC TTTTTTGCCA GAATCTACCA CCCCCTTTGC CACCAAAGAA ATATGCTATA 
ACCAGTGTGC CACAGTCAGA GAAAAGCGAA TCTACACCTG ATGTCAAACT TACAGAGGTG 
TTTAAAGCTA CCTCTCATCT TCCGAAGCAC AGTTTAAGTA CAGCTTCAGA ACCAAGTTTA 
GAAGTGAGTA CACATATGAA TGATGAAAGA CATAAAGAAA CATTTCAAGT GAGAGAATGT 
TTTGGCAACA CACCAAACTG TCCATCCAGC TCCTCAACTA ACGATTTTCA GGCAAACTCA 
GGTGCCATTG ATGCATTTTG CCAACCAGAA CTAGACTCTA TTTCTACCTG TCCAAATGAG 
ACAGTTTCAT TAACTACCTA TTTTTCAGTT GATAGCTGCA TGACGGATAC ATATAGATTG 
AAATACCATC AGAGGCCCAA GCTCTCTTTT CCAGAGAGCA GTGGCTTTTG TAATAATTCA 
CTATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( XM_017008316.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863805.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu38975
Clone ID Related Accession (Same CDS sequence)	NM_001371396.1 , XM_005263076.2 , XM_017008316.1 , XM_017008316.2 , XM_005263076.3
Accession Version	XM_017008316.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017008316.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGCTAAG 
TTAAGTCACA TTGATCAAAG GGAAAAGATA AAAGACATTT CCAGAGAATG TGCTCTGAAA 
GCTATTGAAC AGAAAAACTT ACTTTCTTCA CAAAGGAAAG ATTTAGAGAA GGGACAAAGA 
AAAGATTTAG GACGACATAG AGATTTGGTT GATGAAGACC TTTCACATTT CCAATCTGGA 
TCACCTCCTG CCCCAAATGG TTTTAAACAA CATGGGAATC CACATCTATA TCATAGTCAA 
GGAAAAGGAT CATATAAACA TGACCGAGTT GTACCTCAGA GTCGAGCTTC TGCACAAATA 
ATAAGTTCAA GTAAATCCCA GATTCTTGCT CCAGGAGAGA AAATAACTGG CAAAGTTAAG 
AGTGACAATG GCACTGGATA TGACACAGAC AGCAGCCAAG ATTCTAGGGA TAGAGGAAAC 
AGCTGTGATA GCAGCAGTAA AAGCCGGAAC CGAGGTTGGA AACCTATGAG AGAAACATTA 
AATGTTGATA GTATTTTTAG TGAAAGTGAA AAAAGACAGC ATAGTCCAAG ACATAAACCA 
AATATCAGTA ATAAGCCTAA ATCTAGCAAG GATCCGAGTT TTAGTAATTG GCCAAAAGAG 
AATCCAAAGC AAAAAGGTTT AATGACCATA TATGAAGATG AAATGAAGCA GGAAATAGGA 
AGCAGAAGTT CCCTTGAATC TAATGGAAAA GGAGCAGAGA AAAATAAAGG CCTTGTAGAG 
GGTAAAGTGC ATGGTGATAA TTGGCAGATG CAAAGGACTG AGTCTGGATA TGAAAGCAGT 
GATCACATCA GTAATGGTTC TACTAATTTG GACTCACCTG TTATCGATGG AAATGGTACA 
GTAATGGATA TCAGTGGTGT TAAAGAAACA GTATGCTTCA GTGACCAGAT TACGACAAGC 
AACCTAAATA AAGAACGTGG GGACTGTACC TCCCTTCAGA GCCAACATCA CTTAGAAGGC 
TTTAGAAAAG AACTCAGGAA TTTGGAAGCA GGCTATAAAT CTCATGAATT CCACCCAGAA 
TCACATTTAC AAATAAAAAA TCATTTGATA AAAAGATCAC ATGTACATGA AGACAATGGA 
AAGTTATTTC CTTCATCCAG TCTACAAATA CCCAAGGACC ATAATGCAAG AGAACATATC 
CACCAGTCAG ATGAACAGAA ACTTGAAAAA CCGAATGAAT GCAAATTTTC TGAGTGGCTT 
AATATAGAAA ATTCTGAGAG AACAGGTTTG CCTTTTCACG TTGATAACTC TGCTTCTGGG 
AAGAGAGTGA ACAGTAATGA ACCATCTTCA TTATGGTCTT CACACCTAAG AACTGTTGGG 
TTAAAGCCAG AAACTGCTCC TCTCATCCAG CAACAAAATA TCATGGATCA ATGTTACTTT 
GAGAACTCTC TATCCACAGA ATGTATAATT CGGTCAGCCA GCAGATCTGA TGGGTGTCAG 
ATGCCAAAAC TTTTTTGCCA GAATCTACCA CCCCCTTTGC CACCAAAGAA ATATGCTATA 
ACCAGTGTGC CACAGTCAGA GAAAAGCGAA TCTACACCTG ATGTCAAACT TACAGAGGTG 
TTTAAAGCTA CCTCTCATCT TCCGAAGCAC AGTTTAAGTA CAGCTTCAGA ACCAAGTTTA 
GAAGTGAGTA CACATATGAA TGATGAAAGA CATAAAGAAA CATTTCAAGT GAGAGAATGT 
TTTGGCAACA CACCAAACTG TCCATCCAGC TCCTCAACTA ACGATTTTCA GGCAAACTCA 
GGTGCCATTG ATGCATTTTG CCAACCAGAA CTAGACTCTA TTTCTACCTG TCCAAATGAG 
ACAGTTTCAT TAACTACCTA TTTTTCAGTT GATAGCTGCA TGACGGATAC ATATAGATTG 
AAATACCATC AGAGGCCCAA GCTCTCTTTT CCAGAGAGCA GTGGCTTTTG TAATAATTCA 
CTATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863805.1
CDS	899..3967
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEGFRKELRNLEAGYKSHEFHPESHLQIKNHLIKRSHVHEDNGKLFPSSSLQIPKDHNAREHIHQSDEQKLEKPNECKFSEWLNIENSERTGLPFHVDNSASGKRVNSNEPSSLWSSHLRTVGLKPETAPLIQQQNIMDQCYFENSLSTECIIRSASRSDGCQMPKLFCQNLPPPLPPKKYAITSVPQSEKSESTPDVKLTEVFKATSHLPKHSLSTASEPSLEVSTHMNDERHKETFQVRECFGNTPNCPSSSSTNDFQANSGAIDAFCQPELDSISTCPNETVSLTTYFSVDSCMTDTYRLKYHQRPKLSFPESSGFCNNSLS

Target ORF information:

RefSeq Version	XM_017008316.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008316.2

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGCTAAG 
TTAAGTCACA TTGATCAAAG GGAAAAGATA AAAGACATTT CCAGAGAATG TGCTCTGAAA 
GCTATTGAAC AGAAAAACTT ACTTTCTTCA CAAAGGAAAG ATTTAGAGAA GGGACAAAGA 
AAAGATTTAG GACGACATAG AGATTTGGTT GATGAAGACC TTTCACATTT CCAATCTGGA 
TCACCTCCTG CCCCAAATGG TTTTAAACAA CATGGGAATC CACATCTATA TCATAGTCAA 
GGAAAAGGAT CATATAAACA TGACCGAGTT GTACCTCAGA GTCGAGCTTC TGCACAAATA 
ATAAGTTCAA GTAAATCCCA GATTCTTGCT CCAGGAGAGA AAATAACTGG CAAAGTTAAG 
AGTGACAATG GCACTGGATA TGACACAGAC AGCAGCCAAG ATTCTAGGGA TAGAGGAAAC 
AGCTGTGATA GCAGCAGTAA AAGCCGGAAC CGAGGTTGGA AACCTATGAG AGAAACATTA 
AATGTTGATA GTATTTTTAG TGAAAGTGAA AAAAGACAGC ATAGTCCAAG ACATAAACCA 
AATATCAGTA ATAAGCCTAA ATCTAGCAAG GATCCGAGTT TTAGTAATTG GCCAAAAGAG 
AATCCAAAGC AAAAAGGTTT AATGACCATA TATGAAGATG AAATGAAGCA GGAAATAGGA 
AGCAGAAGTT CCCTTGAATC TAATGGAAAA GGAGCAGAGA AAAATAAAGG CCTTGTAGAG 
GGTAAAGTGC ATGGTGATAA TTGGCAGATG CAAAGGACTG AGTCTGGATA TGAAAGCAGT 
GATCACATCA GTAATGGTTC TACTAATTTG GACTCACCTG TTATCGATGG AAATGGTACA 
GTAATGGATA TCAGTGGTGT TAAAGAAACA GTATGCTTCA GTGACCAGAT TACGACAAGC 
AACCTAAATA AAGAACGTGG GGACTGTACC TCCCTTCAGA GCCAACATCA CTTAGAAGGC 
TTTAGAAAAG AACTCAGGAA TTTGGAAGCA GGCTATAAAT CTCATGAATT CCACCCAGAA 
TCACATTTAC AAATAAAAAA TCATTTGATA AAAAGATCAC ATGTACATGA AGACAATGGA 
AAGTTATTTC CTTCATCCAG TCTACAAATA CCCAAGGACC ATAATGCAAG AGAACATATC 
CACCAGTCAG ATGAACAGAA ACTTGAAAAA CCGAATGAAT GCAAATTTTC TGAGTGGCTT 
AATATAGAAA ATTCTGAGAG AACAGGTTTG CCTTTTCACG TTGATAACTC TGCTTCTGGG 
AAGAGAGTGA ACAGTAATGA ACCATCTTCA TTATGGTCTT CACACCTAAG AACTGTTGGG 
TTAAAGCCAG AAACTGCTCC TCTCATCCAG CAACAAAATA TCATGGATCA ATGTTACTTT 
GAGAACTCTC TATCCACAGA ATGTATAATT CGGTCAGCCA GCAGATCTGA TGGGTGTCAG 
ATGCCAAAAC TTTTTTGCCA GAATCTACCA CCCCCTTTGC CACCAAAGAA ATATGCTATA 
ACCAGTGTGC CACAGTCAGA GAAAAGCGAA TCTACACCTG ATGTCAAACT TACAGAGGTG 
TTTAAAGCTA CCTCTCATCT TCCGAAGCAC AGTTTAAGTA CAGCTTCAGA ACCAAGTTTA 
GAAGTGAGTA CACATATGAA TGATGAAAGA CATAAAGAAA CATTTCAAGT GAGAGAATGT 
TTTGGCAACA CACCAAACTG TCCATCCAGC TCCTCAACTA ACGATTTTCA GGCAAACTCA 
GGTGCCATTG ATGCATTTTG CCAACCAGAA CTAGACTCTA TTTCTACCTG TCCAAATGAG 
ACAGTTTCAT TAACTACCTA TTTTTCAGTT GATAGCTGCA TGACGGATAC ATATAGATTG 
AAATACCATC AGAGGCCCAA GCTCTCTTTT CCAGAGAGCA GTGGCTTTTG TAATAATTCA 
CTATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( XM_005263076.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263133.1 Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X8, mRNA.

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CloneID	OHu38975
Clone ID Related Accession (Same CDS sequence)	NM_001371396.1 , XM_005263076.2 , XM_017008316.1 , XM_017008316.2 , XM_005263076.3
Accession Version	XM_005263076.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005263076.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGCTAAG 
TTAAGTCACA TTGATCAAAG GGAAAAGATA AAAGACATTT CCAGAGAATG TGCTCTGAAA 
GCTATTGAAC AGAAAAACTT ACTTTCTTCA CAAAGGAAAG ATTTAGAGAA GGGACAAAGA 
AAAGATTTAG GACGACATAG AGATTTGGTT GATGAAGACC TTTCACATTT CCAATCTGGA 
TCACCTCCTG CCCCAAATGG TTTTAAACAA CATGGGAATC CACATCTATA TCATAGTCAA 
GGAAAAGGAT CATATAAACA TGACCGAGTT GTACCTCAGA GTCGAGCTTC TGCACAAATA 
ATAAGTTCAA GTAAATCCCA GATTCTTGCT CCAGGAGAGA AAATAACTGG CAAAGTTAAG 
AGTGACAATG GCACTGGATA TGACACAGAC AGCAGCCAAG ATTCTAGGGA TAGAGGAAAC 
AGCTGTGATA GCAGCAGTAA AAGCCGGAAC CGAGGTTGGA AACCTATGAG AGAAACATTA 
AATGTTGATA GTATTTTTAG TGAAAGTGAA AAAAGACAGC ATAGTCCAAG ACATAAACCA 
AATATCAGTA ATAAGCCTAA ATCTAGCAAG GATCCGAGTT TTAGTAATTG GCCAAAAGAG 
AATCCAAAGC AAAAAGGTTT AATGACCATA TATGAAGATG AAATGAAGCA GGAAATAGGA 
AGCAGAAGTT CCCTTGAATC TAATGGAAAA GGAGCAGAGA AAAATAAAGG CCTTGTAGAG 
GGTAAAGTGC ATGGTGATAA TTGGCAGATG CAAAGGACTG AGTCTGGATA TGAAAGCAGT 
GATCACATCA GTAATGGTTC TACTAATTTG GACTCACCTG TTATCGATGG AAATGGTACA 
GTAATGGATA TCAGTGGTGT TAAAGAAACA GTATGCTTCA GTGACCAGAT TACGACAAGC 
AACCTAAATA AAGAACGTGG GGACTGTACC TCCCTTCAGA GCCAACATCA CTTAGAAGGC 
TTTAGAAAAG AACTCAGGAA TTTGGAAGCA GGCTATAAAT CTCATGAATT CCACCCAGAA 
TCACATTTAC AAATAAAAAA TCATTTGATA AAAAGATCAC ATGTACATGA AGACAATGGA 
AAGTTATTTC CTTCATCCAG TCTACAAATA CCCAAGGACC ATAATGCAAG AGAACATATC 
CACCAGTCAG ATGAACAGAA ACTTGAAAAA CCGAATGAAT GCAAATTTTC TGAGTGGCTT 
AATATAGAAA ATTCTGAGAG AACAGGTTTG CCTTTTCACG TTGATAACTC TGCTTCTGGG 
AAGAGAGTGA ACAGTAATGA ACCATCTTCA TTATGGTCTT CACACCTAAG AACTGTTGGG 
TTAAAGCCAG AAACTGCTCC TCTCATCCAG CAACAAAATA TCATGGATCA ATGTTACTTT 
GAGAACTCTC TATCCACAGA ATGTATAATT CGGTCAGCCA GCAGATCTGA TGGGTGTCAG 
ATGCCAAAAC TTTTTTGCCA GAATCTACCA CCCCCTTTGC CACCAAAGAA ATATGCTATA 
ACCAGTGTGC CACAGTCAGA GAAAAGCGAA TCTACACCTG ATGTCAAACT TACAGAGGTG 
TTTAAAGCTA CCTCTCATCT TCCGAAGCAC AGTTTAAGTA CAGCTTCAGA ACCAAGTTTA 
GAAGTGAGTA CACATATGAA TGATGAAAGA CATAAAGAAA CATTTCAAGT GAGAGAATGT 
TTTGGCAACA CACCAAACTG TCCATCCAGC TCCTCAACTA ACGATTTTCA GGCAAACTCA 
GGTGCCATTG ATGCATTTTG CCAACCAGAA CTAGACTCTA TTTCTACCTG TCCAAATGAG 
ACAGTTTCAT TAACTACCTA TTTTTCAGTT GATAGCTGCA TGACGGATAC ATATAGATTG 
AAATACCATC AGAGGCCCAA GCTCTCTTTT CCAGAGAGCA GTGGCTTTTG TAATAATTCA 
CTATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263133.1
CDS	1067..4135
Translation	MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLFYRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMAKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLYHSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDSRDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPSFSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQRTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDCTSLQSQHHLEGFRKELRNLEAGYKSHEFHPESHLQIKNHLIKRSHVHEDNGKLFPSSSLQIPKDHNAREHIHQSDEQKLEKPNECKFSEWLNIENSERTGLPFHVDNSASGKRVNSNEPSSLWSSHLRTVGLKPETAPLIQQQNIMDQCYFENSLSTECIIRSASRSDGCQMPKLFCQNLPPPLPPKKYAITSVPQSEKSESTPDVKLTEVFKATSHLPKHSLSTASEPSLEVSTHMNDERHKETFQVRECFGNTPNCPSSSSTNDFQANSGAIDAFCQPELDSISTCPNETVSLTTYFSVDSCMTDTYRLKYHQRPKLSFPESSGFCNNSLS

Target ORF information:

RefSeq Version	XM_005263076.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263076.3

ORF Insert Sequence:

ATGGCATGGG TAAAATTCTT ACGGAAACCT GGTGGCAATC TTGGAAAAGT TTATCAGCCT 
GGAAGTATGC TATCACTAGC CCCTACCAAA GGCTTGTTAA ATGAACCAGG ACAAAACAGC 
TGCTTTCTTA ATAGCGCTGT ACAGGTTTTA TGGCAATTGG ATATATTCCG ACGAAGCTTG 
CGGGTTTTGA CTGGACATGT TTGTCAGGGA GATGCCTGTA TATTTTGTGC ATTGAAGACG 
ATATTTGCAC AGTTCCAACA CAGTCGAGAA AAAGCACTTC CCTCAGATAA CATAAGGCAT 
GCTCTTGCAG AAAGTTTCAA AGATGAGCAG CGATTTCAAC TTGGCCTTAT GGATGATGCT 
GCGGAGTGCT TTGAAAATAT GTTGGAGAGG ATTCATTTTC ACATAGTGCC AAGCAGAGAT 
GCAGACATGT GTACCTCTAA ATCTTGTATC ACTCACCAGA AGTTTGCTAT GACTCTGTAT 
GAACAGTGTG TGTGTCGTAG CTGTGGAGCA TCGTCAGATC CTCTACCTTT TACAGAATTT 
GTGCGGTACA TTTCTACAAC AGCCTTATGC AATGAGGTTG AAAGAATGTT GGAAAGGCAT 
GAACGCTTTA AACCTGAAAT GTTTGCAGAA TTGCTACAAG CAGCAAATAC AACAGATGAC 
TATAGGAAAT GTCCTAGTAA CTGTGGCCAA AAAATAAAAA TTCGCCGTGT TTTAATGAAT 
TGCCCAGAGA TTGTTACAAT TGGTTTAGTC TGGGACTCCG AGCATTCTGA CTTGACCGAA 
GCTGTTGTTC GGAATCTAGC AACACATCTT TATCTTCCTG GGCTTTTTTA TAGAGTTACT 
GATGAAAATG CCAAAAATAG TGAACTTAAC CTTGTTGGTA TGATCTGCTA CACCAGCCAA 
CATTATTGTG CCTTTGCATT TCACACCAAA AGTTCCAAAT GGGTATTTTT TGATGATGCA 
AATGTGAAAG AGATTGGAAC TAGATGGAAA GATGTTGTCT CCAAATGCAT TCGATGCCAC 
TTTCAGCCAC TACTTTTGTT TTATGCAAAC CCAGATGGCA CAGCAGTTTC TACTGAGGAT 
GCACTCAGGC AGGTCATCAG CTGGTCACAT TACAAATCTG TTGCAGAAAA TATGGCTAAG 
TTAAGTCACA TTGATCAAAG GGAAAAGATA AAAGACATTT CCAGAGAATG TGCTCTGAAA 
GCTATTGAAC AGAAAAACTT ACTTTCTTCA CAAAGGAAAG ATTTAGAGAA GGGACAAAGA 
AAAGATTTAG GACGACATAG AGATTTGGTT GATGAAGACC TTTCACATTT CCAATCTGGA 
TCACCTCCTG CCCCAAATGG TTTTAAACAA CATGGGAATC CACATCTATA TCATAGTCAA 
GGAAAAGGAT CATATAAACA TGACCGAGTT GTACCTCAGA GTCGAGCTTC TGCACAAATA 
ATAAGTTCAA GTAAATCCCA GATTCTTGCT CCAGGAGAGA AAATAACTGG CAAAGTTAAG 
AGTGACAATG GCACTGGATA TGACACAGAC AGCAGCCAAG ATTCTAGGGA TAGAGGAAAC 
AGCTGTGATA GCAGCAGTAA AAGCCGGAAC CGAGGTTGGA AACCTATGAG AGAAACATTA 
AATGTTGATA GTATTTTTAG TGAAAGTGAA AAAAGACAGC ATAGTCCAAG ACATAAACCA 
AATATCAGTA ATAAGCCTAA ATCTAGCAAG GATCCGAGTT TTAGTAATTG GCCAAAAGAG 
AATCCAAAGC AAAAAGGTTT AATGACCATA TATGAAGATG AAATGAAGCA GGAAATAGGA 
AGCAGAAGTT CCCTTGAATC TAATGGAAAA GGAGCAGAGA AAAATAAAGG CCTTGTAGAG 
GGTAAAGTGC ATGGTGATAA TTGGCAGATG CAAAGGACTG AGTCTGGATA TGAAAGCAGT 
GATCACATCA GTAATGGTTC TACTAATTTG GACTCACCTG TTATCGATGG AAATGGTACA 
GTAATGGATA TCAGTGGTGT TAAAGAAACA GTATGCTTCA GTGACCAGAT TACGACAAGC 
AACCTAAATA AAGAACGTGG GGACTGTACC TCCCTTCAGA GCCAACATCA CTTAGAAGGC 
TTTAGAAAAG AACTCAGGAA TTTGGAAGCA GGCTATAAAT CTCATGAATT CCACCCAGAA 
TCACATTTAC AAATAAAAAA TCATTTGATA AAAAGATCAC ATGTACATGA AGACAATGGA 
AAGTTATTTC CTTCATCCAG TCTACAAATA CCCAAGGACC ATAATGCAAG AGAACATATC 
CACCAGTCAG ATGAACAGAA ACTTGAAAAA CCGAATGAAT GCAAATTTTC TGAGTGGCTT 
AATATAGAAA ATTCTGAGAG AACAGGTTTG CCTTTTCACG TTGATAACTC TGCTTCTGGG 
AAGAGAGTGA ACAGTAATGA ACCATCTTCA TTATGGTCTT CACACCTAAG AACTGTTGGG 
TTAAAGCCAG AAACTGCTCC TCTCATCCAG CAACAAAATA TCATGGATCA ATGTTACTTT 
GAGAACTCTC TATCCACAGA ATGTATAATT CGGTCAGCCA GCAGATCTGA TGGGTGTCAG 
ATGCCAAAAC TTTTTTGCCA GAATCTACCA CCCCCTTTGC CACCAAAGAA ATATGCTATA 
ACCAGTGTGC CACAGTCAGA GAAAAGCGAA TCTACACCTG ATGTCAAACT TACAGAGGTG 
TTTAAAGCTA CCTCTCATCT TCCGAAGCAC AGTTTAAGTA CAGCTTCAGA ACCAAGTTTA 
GAAGTGAGTA CACATATGAA TGATGAAAGA CATAAAGAAA CATTTCAAGT GAGAGAATGT 
TTTGGCAACA CACCAAACTG TCCATCCAGC TCCTCAACTA ACGATTTTCA GGCAAACTCA 
GGTGCCATTG ATGCATTTTG CCAACCAGAA CTAGACTCTA TTTCTACCTG TCCAAATGAG 
ACAGTTTCAT TAACTACCTA TTTTTCAGTT GATAGCTGCA TGACGGATAC ATATAGATTG 
AAATACCATC AGAGGCCCAA GCTCTCTTTT CCAGAGAGCA GTGGCTTTTG TAATAATTCA 
CTATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

Identification of a candidate gene for astigmatism.
Investigative ophthalmology & visual science54(2)1260-7(2013 Feb)
Lopes MC,Hysi PG,Verhoeven VJ,Macgregor S,Hewitt AW,Montgomery GW,Cumberland P,Vingerling JR,Young TL,van Duijn CM,Oostra B,Uitterlinden AG,Rahi JS,Mackey DA,Klaver CC,Andrew T,Hammond CJ

A systematic gene-based screen of chr4q22-q32 identifies association of a novel susceptibility gene, DKK2, with the quantitative trait of alcohol dependence symptom counts.
Human molecular genetics19(12)2497-506(2010 Jun)
Kalsi G,Kuo PH,Aliev F,Alexander J,McMichael O,Patterson DG,Walsh D,Zhao Z,Schuckit M,Nurnberger J Jr,Edenberg H,Kramer J,Hesselbrock V,Tischfield JA,Vladimirov V,Prescott CA,Dick DM,Kendler KS,Riley BP

The lacrimal gland transcriptome is an unusually rich source of rare and poorly characterized gene transcripts.
Investigative ophthalmology & visual science46(5)1572-80(2005 May)
Ozyildirim AM,Wistow GJ,Gao J,Wang J,Dickinson DP,Frierson HF Jr,Laurie GW

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USP53 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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