MTRF1L cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MTRF1L
Entrez Gene ID	54516
Full Name	mitochondrial translational release factor 1 like
Synonyms	HMRF1L,MRF1L,mtRF1a
General protein information	Preferred Names mitochondrial translational release factor 1 like Names peptide chain release factor 1-like, mitochondrial mitochondrial release factor 1 like
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q25.2
Summary	The protein encoded by this gene plays a role in mitochondrial translation termination, and is thought to be a release factor that is involved in the dissociation of the complete protein from the final tRNA, the ribosome, and the cognate mRNA. This protein acts upon UAA and UAG stop codons, but has no in vitro activity against UGA, which encodes tryptophan in human mitochondrion, or, the mitochondrial non-cognate stop codons, AGA and AGG. This protein shares sequence similarity to bacterial release factors. Pseudogenes of this gene are found on chromosomes 4, 8, and 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014].
Disorder MIM:	613542

mRNA and Protein(s)

mRNA	Protein	Name
NM_001301872.1	NP_001288801.1	peptide chain release factor 1-like, mitochondrial isoform 6
XM_017010960.1	XP_016866449.1	peptide chain release factor 1-like, mitochondrial isoform X1
NM_001301047.3	NP_001287976.1	peptide chain release factor 1-like, mitochondrial isoform 5
XM_017010959.1	XP_016866448.1	peptide chain release factor 1-like, mitochondrial isoform X1
NM_001114184.2	NP_001107656.1	peptide chain release factor 1-like, mitochondrial isoform 3
NM_001301870.1	NP_001288799.1	peptide chain release factor 1-like, mitochondrial isoform 2
NM_019041.7	NP_061914.3	peptide chain release factor 1-like, mitochondrial isoform 1
NM_001301870.2	NP_001288799.1	peptide chain release factor 1-like, mitochondrial isoform 2
NM_001301047.2	NP_001287976.1	peptide chain release factor 1-like, mitochondrial isoform 5
NM_019041.6	NP_061914.3	peptide chain release factor 1-like, mitochondrial isoform 1
NM_001301871.1	NP_001288800.1	peptide chain release factor 1-like, mitochondrial isoform 4
NM_001301871.2	NP_001288800.1	peptide chain release factor 1-like, mitochondrial isoform 4
NM_001114184.3	NP_001107656.1	peptide chain release factor 1-like, mitochondrial isoform 3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MTRF1L	XP_541157.3
Danio rerio (zebrafish)	LOC100333733	XP_002664272.1
Drosophila melanogaster (fruit fly)	CG5705	NP_609617.1
Homo sapiens (human)	MTRF1L	NP_061914.3
Caenorhabditis elegans (roundworm)	W03F8.3	NP_500737.1
Kluyveromyces lactis	KLLA0E14807g	XP_454617.1
Magnaporthe oryzae (rice blast fungus)	MGG_09539	XP_003712202.1
Neurospora crassa	NCU06721	XP_960901.2
Arabidopsis thaliana (thale cress)	APG3	NP_191850.2
Pan troglodytes (chimpanzee)	MTRF1L	XP_518818.2
	LOC698305	XP_001086824.2
Mus musculus (house mouse)	Mtrf1l	NP_780583.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012082	XP_320444.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012078	XP_320450.4
Schizosaccharomyces pombe (fission yeast)	SPAC2F7.17	NP_592987.1
Bos taurus (cattle)	MTRF1L	NP_001039843.1
Rattus norvegicus (Norway rat)	Mtrf1l	NP_001020894.1
Gallus gallus (chicken)	MTRF1L	XP_419682.2
Saccharomyces cerevisiae (baker's yeast)	MRF1	NP_011372.3
Eremothecium gossypii	AGOS_ADL284C	NP_983812.2
Oryza sativa (rice)	Os11g0657100	NP_001068394.1
Xenopus tropicalis (tropical clawed frog)	mtrf1l	XP_002936423.2

Gene Ontology
Bibliography

Related articles in PubMed

Identification of 23 new prostate cancer susceptibility loci using the iCOGS custom genotyping array.
Eeles RA, Olama AA, Benlloch S, Saunders EJ, Leongamornlert DA, Tymrakiewicz M, Ghoussaini M, Luccarini C, Dennis J, Jugurnauth-Little S, Dadaev T, Neal DE, Hamdy FC, Donovan JL, Muir K, Giles GG, Severi G, Wiklund F, Gronberg H, Haiman CA, Schumacher F, Henderson BE, Le Marchand L, Lindstrom S, Kraft P, Hunter DJ, Gapstur S, Chanock SJ, Berndt SI, Albanes D, Andriole G, Schleutker J, Weischer M, Canzian F, Riboli E, Key TJ, Travis RC, Campa D, Ingles SA, John EM, Hayes RB, Pharoah PD, Pashayan N, Khaw KT, Stanford JL, Ostrander EA, Signorello LB, Thibodeau SN, Schaid D, Maier C, Vogel W, Kibel AS, Cybulski C, Lubinski J, Cannon-Albright L, Brenner H, Park JY, Kaneva R, Batra J, Spurdle AB, Clements JA, Teixeira MR, Dicks E, Lee A, Dunning AM, Baynes C, Conroy D, Maranian MJ, Ahmed S, Govindasami K, Guy M, Wilkinson RA, Sawyer EJ, Morgan A, Dearnaley DP, Horwich A, Huddart RA, Khoo VS, Parker CC, Van As NJ, Woodhouse CJ, Thompson A, Dudderidge T, Ogden C, Cooper CS, Lophatananon A, Cox A, Southey MC, Hopper JL, English DR, Aly M, Adolfsson J, Xu J, Zheng SL, Yeager M, Kaaks R, Diver WR, Gaudet MM, Stern MC, Corral R, Joshi AD, Shahabi A, Wahlfors T, Tammela TL, Auvinen A, Virtamo J, Klarskov P, Nordestgaard BG, R?der MA, Nielsen SF, Bojesen SE, Siddiq A, Fitzgerald LM, Kolb S, Kwon EM, Karyadi DM, Blot WJ, Zheng W, Cai Q, McDonnell SK, Rinckleb AE, Drake B, Colditz G, Wokolorczyk D, Stephenson RA, Teerlink C, Muller H, Rothenbacher D, Sellers TA, Lin HY, Slavov C, Mitev V, Lose F, Srinivasan S, Maia S, Paulo P, Lange E, Cooney KA, Antoniou AC, Vincent D, Bacot F, Tessier DC, , , , , , Kote-Jarai Z, Easton DF
Nature genetics45(4)385-91, 391e1-2(2013 Apr)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

Mutations in C12orf65 in patients with encephalomyopathy and a mitochondrial translation defect.
Antonicka H, Ostergaard E, Sasarman F, Weraarpachai W, Wibrand F, Pedersen AM, Rodenburg RJ, van der Knaap MS, Smeitink JA, Chrzanowska-Lightowlers ZM, Shoubridge EA
American journal of human genetics87(1)115-22(2010 Jul)

Candidate gene/loci studies in cleft lip/palate and dental anomalies finds novel susceptibility genes for clefts.
Vieira AR, McHenry TG, Daack-Hirsch S, Murray JC, Marazita ML
Genetics in medicine : official journal of the American College of Medical Genetics10(9)668-74(2008 Sep)

The human mitochondrial translation release factor HMRF1L is methylated in the GGQ motif by the methyltransferase HMPrmC.
Ishizawa T, Nozaki Y, Ueda T, Takeuchi N
Biochemical and biophysical research communications373(1)99-103(2008 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Observational study of gene-disease association. (HuGE Navigator)
Title: Candidate gene/loci studies in cleft lip/palate and dental anomalies finds novel susceptibility genes for clefts.

The methylation of HMRF1L by HMPrmC in human mitochondria is involved in the control of the translation termination process.
Title: The human mitochondrial translation release factor HMRF1L is methylated in the GGQ motif by the methyltransferase HMPrmC.

Study identified a human mitochondrial release factor candidate, HMRF1L, and demonstrated that HMRF1L is indeed a mitochondrial protein that functions specifically as an RF for the decoding of mitochondrial UAA and UAG termination codons in vitro.
Title: HMRF1L is a human mitochondrial translation release factor involved in the decoding of the termination codons UAA and UAG.

mtRF1a is a mitochondrial translation release factor that is capable in vitro and in vivo of terminating translation at UAA/UAG codons.
Title: mtRF1a is a human mitochondrial translation release factor decoding the major termination codons UAA and UAG.

The following MTRF1L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTRF1L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu08868	NM_001114184.3 Latest version!	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu09020	NM_019041.7 Latest version!	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu40858	NM_001301871.2 Latest version!	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu40858	XM_017010959.1 Latest version!	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu40858	XM_017010960.1 Latest version!	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu40857	NM_001301870.2 Latest version!	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu40859	NM_001301047.3 Latest version!	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OHu68403	NM_001301872.1 Latest version!	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
View Old Accession Versions >>
OHu40857	NM_001301870.1	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu40859	NM_001301047.2	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OHu09020	NM_019041.6	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu40858	NM_001301871.1	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu08868	NM_001114184.2	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MTRF1L ( NM_001114184.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001107656.1 Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu08868
Clone ID Related Accession (Same CDS sequence)	NM_001114184.2 , NM_001114184.3
Accession Version	NM_001114184.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	peptide chain release factor 1-like, mitochondrial isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA152954.1, BC014428.1, AI479199.1, BU853336.1 and BX105260.1. On Sep 25, 2014 this sequence version replaced NM_001114184.1. Transcript Variant: This variant (3) lacks an alternate exon in the 3' coding region, which results in a frameshift and an early stop codon, compared to variant 1. This variant encodes isoform 3, which is shorter and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.10582.1, SRR1803611.241813.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGGTCCC GGGTTCTGTG GGGCGCTGCC CGGTGGCTCT GGCCCCGCCG GGCCGTTGGC 
CCAGCCCGCC GGCCCCTGAG CTCCGGTAGC CCGCCGCTGG AGGAGCTGTT CACCCGGGGC 
GGGCCCTTGC GGACCTTCCT CGAGCGCCAG GCGGGGTCTG AAGCCCATTT GAAGGTCAGG 
AGGCCCGAGT TGCTGGCGGT GATCAAACTG CTGAACGAGA AGGAGCGGGA GCTGCGGGAG 
ACTGAGCACT TGCTGCACGA TGAGAATGAA GATTTAAGGA AACTTGCAGA GAATGAAATC 
ACTTTGTGTC AAAAAGAAAT AACTCAGCTG AAGCATCAGA TTATCTTACT TTTGGTTCCC 
TCAGAAGAAA CAGATGAAAA TGATTTGATC CTGGAAGTAA CTGCAGGAGT TGGAGGTCAG 
GAGGCAATGT TGTTTACATC AGAGATATTT GATATGTATC AGCAATATGC TGCATTTAAA 
AGATGGCATT TTGAAACCCT GGAATATTTT CCAAGTGAAC TAGGTGGCCT TAGACATGCA 
TCTGCCAGCA TTGGGGGTTC AGAAGCCTAT AGGCACATGA AATTTGAAGG AGGTGTTCAC 
AGAGTACAAA GAGTGCCAAA GACAGAAAAG CAAGGCCGCG TCCATACTAG CACCATGACT 
GTAGCAATAT TACCCCAGCC TACTGAGATT AATCTGGTGA TTAATCCGAA AGATTTGAGA 
ATTGACACTA AGCGAGCCAG TGGAGCTGGG GGGCAGCATG TAAATACCAC GGACAGTGCT 
GTCCGGATAG TTCATCTTCC AACAGATTGG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107656.1
CDS	131..946
Translation	MRSRVLWGAARWLWPRRAVGPARRPLSSGSPPLEELFTRGGPLRTFLERQAGSEAHLKVRRPELLAVIKLLNEKERELRETEHLLHDENEDLRKLAENEITLCQKEITQLKHQIILLLVPSEETDENDLILEVTAGVGGQEAMLFTSEIFDMYQQYAAFKRWHFETLEYFPSELGGLRHASASIGGSEAYRHMKFEGGVHRVQRVPKTEKQGRVHTSTMTVAILPQPTEINLVINPKDLRIDTKRASGAGGQHVNTTDSAVRIVHLPTDWK

Target ORF information:

RefSeq Version	NM_001114184.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114184.2

ORF Insert Sequence:

ATGCGGTCCC GGGTTCTGTG GGGCGCTGCC CGGTGGCTCT GGCCCCGCCG GGCCGTTGGC 
CCAGCCCGCC GGCCCCTGAG CTCCGGTAGC CCGCCGCTGG AGGAGCTGTT CACCCGGGGC 
GGGCCCTTGC GGACCTTCCT CGAGCGCCAG GCGGGGTCTG AAGCCCATTT GAAGGTCAGG 
AGGCCCGAGT TGCTGGCGGT GATCAAACTG CTGAACGAGA AGGAGCGGGA GCTGCGGGAG 
ACTGAGCACT TGCTGCACGA TGAGAATGAA GATTTAAGGA AACTTGCAGA GAATGAAATC 
ACTTTGTGTC AAAAAGAAAT AACTCAGCTG AAGCATCAGA TTATCTTACT TTTGGTTCCC 
TCAGAAGAAA CAGATGAAAA TGATTTGATC CTGGAAGTAA CTGCAGGAGT TGGAGGTCAG 
GAGGCAATGT TGTTTACATC AGAGATATTT GATATGTATC AGCAATATGC TGCATTTAAA 
AGATGGCATT TTGAAACCCT GGAATATTTT CCAAGTGAAC TAGGTGGCCT TAGACATGCA 
TCTGCCAGCA TTGGGGGTTC AGAAGCCTAT AGGCACATGA AATTTGAAGG AGGTGTTCAC 
AGAGTACAAA GAGTGCCAAA GACAGAAAAG CAAGGCCGCG TCCATACTAG CACCATGACT 
GTAGCAATAT TACCCCAGCC TACTGAGATT AATCTGGTGA TTAATCCGAA AGATTTGAGA 
ATTGACACTA AGCGAGCCAG TGGAGCTGGG GGGCAGCATG TAAATACCAC GGACAGTGCT 
GTCCGGATAG TTCATCTTCC AACAGATTGG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTRF1L ( NM_001114184.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001107656.1 Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu08868
Clone ID Related Accession (Same CDS sequence)	NM_001114184.2 , NM_001114184.3
Accession Version	NM_001114184.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	peptide chain release factor 1-like, mitochondrial isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA152954.1, BC014428.1, AI479199.1, BU853336.1, BX105260.1 and AL080276.9. On May 31, 2019 this sequence version replaced NM_001114184.2. Transcript Variant: This variant (3) lacks an alternate exon in the 3' coding region, which results in a frameshift and an early stop codon, compared to variant 1. This variant encodes isoform 3, which is shorter and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.84466.1, SRR1803617.168541.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGGTCCC GGGTTCTGTG GGGCGCTGCC CGGTGGCTCT GGCCCCGCCG GGCCGTTGGC 
CCAGCCCGCC GGCCCCTGAG CTCCGGTAGC CCGCCGCTGG AGGAGCTGTT CACCCGGGGC 
GGGCCCTTGC GGACCTTCCT CGAGCGCCAG GCGGGGTCTG AAGCCCATTT GAAGGTCAGG 
AGGCCCGAGT TGCTGGCGGT GATCAAACTG CTGAACGAGA AGGAGCGGGA GCTGCGGGAG 
ACTGAGCACT TGCTGCACGA TGAGAATGAA GATTTAAGGA AACTTGCAGA GAATGAAATC 
ACTTTGTGTC AAAAAGAAAT AACTCAGCTG AAGCATCAGA TTATCTTACT TTTGGTTCCC 
TCAGAAGAAA CAGATGAAAA TGATTTGATC CTGGAAGTAA CTGCAGGAGT TGGAGGTCAG 
GAGGCAATGT TGTTTACATC AGAGATATTT GATATGTATC AGCAATATGC TGCATTTAAA 
AGATGGCATT TTGAAACCCT GGAATATTTT CCAAGTGAAC TAGGTGGCCT TAGACATGCA 
TCTGCCAGCA TTGGGGGTTC AGAAGCCTAT AGGCACATGA AATTTGAAGG AGGTGTTCAC 
AGAGTACAAA GAGTGCCAAA GACAGAAAAG CAAGGCCGCG TCCATACTAG CACCATGACT 
GTAGCAATAT TACCCCAGCC TACTGAGATT AATCTGGTGA TTAATCCGAA AGATTTGAGA 
ATTGACACTA AGCGAGCCAG TGGAGCTGGG GGGCAGCATG TAAATACCAC GGACAGTGCT 
GTCCGGATAG TTCATCTTCC AACAGATTGG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107656.1
CDS	25..840
Translation	MRSRVLWGAARWLWPRRAVGPARRPLSSGSPPLEELFTRGGPLRTFLERQAGSEAHLKVRRPELLAVIKLLNEKERELRETEHLLHDENEDLRKLAENEITLCQKEITQLKHQIILLLVPSEETDENDLILEVTAGVGGQEAMLFTSEIFDMYQQYAAFKRWHFETLEYFPSELGGLRHASASIGGSEAYRHMKFEGGVHRVQRVPKTEKQGRVHTSTMTVAILPQPTEINLVINPKDLRIDTKRASGAGGQHVNTTDSAVRIVHLPTDWK

Target ORF information:

RefSeq Version	NM_001114184.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114184.3

ORF Insert Sequence:

ATGCGGTCCC GGGTTCTGTG GGGCGCTGCC CGGTGGCTCT GGCCCCGCCG GGCCGTTGGC 
CCAGCCCGCC GGCCCCTGAG CTCCGGTAGC CCGCCGCTGG AGGAGCTGTT CACCCGGGGC 
GGGCCCTTGC GGACCTTCCT CGAGCGCCAG GCGGGGTCTG AAGCCCATTT GAAGGTCAGG 
AGGCCCGAGT TGCTGGCGGT GATCAAACTG CTGAACGAGA AGGAGCGGGA GCTGCGGGAG 
ACTGAGCACT TGCTGCACGA TGAGAATGAA GATTTAAGGA AACTTGCAGA GAATGAAATC 
ACTTTGTGTC AAAAAGAAAT AACTCAGCTG AAGCATCAGA TTATCTTACT TTTGGTTCCC 
TCAGAAGAAA CAGATGAAAA TGATTTGATC CTGGAAGTAA CTGCAGGAGT TGGAGGTCAG 
GAGGCAATGT TGTTTACATC AGAGATATTT GATATGTATC AGCAATATGC TGCATTTAAA 
AGATGGCATT TTGAAACCCT GGAATATTTT CCAAGTGAAC TAGGTGGCCT TAGACATGCA 
TCTGCCAGCA TTGGGGGTTC AGAAGCCTAT AGGCACATGA AATTTGAAGG AGGTGTTCAC 
AGAGTACAAA GAGTGCCAAA GACAGAAAAG CAAGGCCGCG TCCATACTAG CACCATGACT 
GTAGCAATAT TACCCCAGCC TACTGAGATT AATCTGGTGA TTAATCCGAA AGATTTGAGA 
ATTGACACTA AGCGAGCCAG TGGAGCTGGG GGGCAGCATG TAAATACCAC GGACAGTGCT 
GTCCGGATAG TTCATCTTCC AACAGATTGG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTRF1L ( NM_019041.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_061914.3 Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09020
Clone ID Related Accession (Same CDS sequence)	NM_019041.7 , NM_019041.6
Accession Version	NM_019041.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	peptide chain release factor 1-like, mitochondrial isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA152954.1, BC011873.2, AK095240.1, AI434611.1, AI479199.1, BU853336.1, BX105260.1 and AL080276.9. On Nov 22, 2018 this sequence version replaced NM_019041.6. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1803613.720.1, SRR1660809.63983.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000367233.10/ ENSP00000356202.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGGTCCC GGGTTCTGTG GGGCGCTGCC CGGTGGCTCT GGCCCCGCCG GGCCGTTGGC 
CCAGCCCGCC GGCCCCTGAG CTCCGGTAGC CCGCCGCTGG AGGAGCTGTT CACCCGGGGC 
GGGCCCTTGC GGACCTTCCT CGAGCGCCAG GCGGGGTCTG AAGCCCATTT GAAGGTCAGG 
AGGCCCGAGT TGCTGGCGGT GATCAAACTG CTGAACGAGA AGGAGCGGGA GCTGCGGGAG 
ACTGAGCACT TGCTGCACGA TGAGAATGAA GATTTAAGGA AACTTGCAGA GAATGAAATC 
ACTTTGTGTC AAAAAGAAAT AACTCAGCTG AAGCATCAGA TTATCTTACT TTTGGTTCCC 
TCAGAAGAAA CAGATGAAAA TGATTTGATC CTGGAAGTAA CTGCAGGAGT TGGAGGTCAG 
GAGGCAATGT TGTTTACATC AGAGATATTT GATATGTATC AGCAATATGC TGCATTTAAA 
AGATGGCATT TTGAAACCCT GGAATATTTT CCAAGTGAAC TAGGTGGCCT TAGACATGCA 
TCTGCCAGCA TTGGGGGTTC AGAAGCCTAT AGGCACATGA AATTTGAAGG AGGTGTTCAC 
AGAGTACAAA GAGTGCCAAA GACAGAAAAG CAAGGCCGCG TCCATACTAG CACCATGACT 
GTAGCAATAT TACCCCAGCC TACTGAGATT AATCTGGTGA TTAATCCGAA AGATTTGAGA 
ATTGACACTA AGCGAGCCAG TGGAGCTGGG GGGCAGCATG TAAATACCAC GGACAGTGCT 
GTCCGGATAG TTCATCTTCC AACAGGTGTT GTTTCTGAAT GTCAACAAGA GAGATCTCAG 
CTGAAAAATA AAGAGCTGGC TATGACAAAG TTACGTGCAA AACTGTACAG CATGCATCTA 
GAAGAAGAAA TAAATAAAAG ACAGAATGCT AGAAAAATTC AGATTGGAAG TAAAGGAAGA 
TCAGAGAAAA TAAGAACATA TAATTTTCCA CAGAACCGGG TCACAGATCA CAGAATAAAC 
AAGACGCTGC ATGATCTTGA AACTTTTATG CAAGGAGATT ATCTACTGGA TGAACTTGTA 
CAGTCATTGA AGGAATACGC CGATTATGAA TCTTTAGTAG AAATTATTTC CCAAAAAGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061914.3
CDS	25..1167
Translation	MRSRVLWGAARWLWPRRAVGPARRPLSSGSPPLEELFTRGGPLRTFLERQAGSEAHLKVRRPELLAVIKLLNEKERELRETEHLLHDENEDLRKLAENEITLCQKEITQLKHQIILLLVPSEETDENDLILEVTAGVGGQEAMLFTSEIFDMYQQYAAFKRWHFETLEYFPSELGGLRHASASIGGSEAYRHMKFEGGVHRVQRVPKTEKQGRVHTSTMTVAILPQPTEINLVINPKDLRIDTKRASGAGGQHVNTTDSAVRIVHLPTGVVSECQQERSQLKNKELAMTKLRAKLYSMHLEEEINKRQNARKIQIGSKGRSEKIRTYNFPQNRVTDHRINKTLHDLETFMQGDYLLDELVQSLKEYADYESLVEIISQKV

Target ORF information:

RefSeq Version	NM_019041.7
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019041.7

ORF Insert Sequence:

ATGCGGTCCC GGGTTCTGTG GGGCGCTGCC CGGTGGCTCT GGCCCCGCCG GGCCGTTGGC 
CCAGCCCGCC GGCCCCTGAG CTCCGGTAGC CCGCCGCTGG AGGAGCTGTT CACCCGGGGC 
GGGCCCTTGC GGACCTTCCT CGAGCGCCAG GCGGGGTCTG AAGCCCATTT GAAGGTCAGG 
AGGCCCGAGT TGCTGGCGGT GATCAAACTG CTGAACGAGA AGGAGCGGGA GCTGCGGGAG 
ACTGAGCACT TGCTGCACGA TGAGAATGAA GATTTAAGGA AACTTGCAGA GAATGAAATC 
ACTTTGTGTC AAAAAGAAAT AACTCAGCTG AAGCATCAGA TTATCTTACT TTTGGTTCCC 
TCAGAAGAAA CAGATGAAAA TGATTTGATC CTGGAAGTAA CTGCAGGAGT TGGAGGTCAG 
GAGGCAATGT TGTTTACATC AGAGATATTT GATATGTATC AGCAATATGC TGCATTTAAA 
AGATGGCATT TTGAAACCCT GGAATATTTT CCAAGTGAAC TAGGTGGCCT TAGACATGCA 
TCTGCCAGCA TTGGGGGTTC AGAAGCCTAT AGGCACATGA AATTTGAAGG AGGTGTTCAC 
AGAGTACAAA GAGTGCCAAA GACAGAAAAG CAAGGCCGCG TCCATACTAG CACCATGACT 
GTAGCAATAT TACCCCAGCC TACTGAGATT AATCTGGTGA TTAATCCGAA AGATTTGAGA 
ATTGACACTA AGCGAGCCAG TGGAGCTGGG GGGCAGCATG TAAATACCAC GGACAGTGCT 
GTCCGGATAG TTCATCTTCC AACAGGTGTT GTTTCTGAAT GTCAACAAGA GAGATCTCAG 
CTGAAAAATA AAGAGCTGGC TATGACAAAG TTACGTGCAA AACTGTACAG CATGCATCTA 
GAAGAAGAAA TAAATAAAAG ACAGAATGCT AGAAAAATTC AGATTGGAAG TAAAGGAAGA 
TCAGAGAAAA TAAGAACATA TAATTTTCCA CAGAACCGGG TCACAGATCA CAGAATAAAC 
AAGACGCTGC ATGATCTTGA AACTTTTATG CAAGGAGATT ATCTACTGGA TGAACTTGTA 
CAGTCATTGA AGGAATACGC CGATTATGAA TCTTTAGTAG AAATTATTTC CCAAAAAGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTRF1L ( NM_019041.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_061914.3 Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09020
Clone ID Related Accession (Same CDS sequence)	NM_019041.7 , NM_019041.6
Accession Version	NM_019041.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	peptide chain release factor 1-like, mitochondrial isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA152954.1, BC011873.2, AK095240.1, AI434611.1, AI479199.1, BU853336.1 and BX105260.1. On Sep 25, 2014 this sequence version replaced NM_019041.5. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1803613.720.1, SRR1660809.63983.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGGTCCC GGGTTCTGTG GGGCGCTGCC CGGTGGCTCT GGCCCCGCCG GGCCGTTGGC 
CCAGCCCGCC GGCCCCTGAG CTCCGGTAGC CCGCCGCTGG AGGAGCTGTT CACCCGGGGC 
GGGCCCTTGC GGACCTTCCT CGAGCGCCAG GCGGGGTCTG AAGCCCATTT GAAGGTCAGG 
AGGCCCGAGT TGCTGGCGGT GATCAAACTG CTGAACGAGA AGGAGCGGGA GCTGCGGGAG 
ACTGAGCACT TGCTGCACGA TGAGAATGAA GATTTAAGGA AACTTGCAGA GAATGAAATC 
ACTTTGTGTC AAAAAGAAAT AACTCAGCTG AAGCATCAGA TTATCTTACT TTTGGTTCCC 
TCAGAAGAAA CAGATGAAAA TGATTTGATC CTGGAAGTAA CTGCAGGAGT TGGAGGTCAG 
GAGGCAATGT TGTTTACATC AGAGATATTT GATATGTATC AGCAATATGC TGCATTTAAA 
AGATGGCATT TTGAAACCCT GGAATATTTT CCAAGTGAAC TAGGTGGCCT TAGACATGCA 
TCTGCCAGCA TTGGGGGTTC AGAAGCCTAT AGGCACATGA AATTTGAAGG AGGTGTTCAC 
AGAGTACAAA GAGTGCCAAA GACAGAAAAG CAAGGCCGCG TCCATACTAG CACCATGACT 
GTAGCAATAT TACCCCAGCC TACTGAGATT AATCTGGTGA TTAATCCGAA AGATTTGAGA 
ATTGACACTA AGCGAGCCAG TGGAGCTGGG GGGCAGCATG TAAATACCAC GGACAGTGCT 
GTCCGGATAG TTCATCTTCC AACAGGTGTT GTTTCTGAAT GTCAACAAGA GAGATCTCAG 
CTGAAAAATA AAGAGCTGGC TATGACAAAG TTACGTGCAA AACTGTACAG CATGCATCTA 
GAAGAAGAAA TAAATAAAAG ACAGAATGCT AGAAAAATTC AGATTGGAAG TAAAGGAAGA 
TCAGAGAAAA TAAGAACATA TAATTTTCCA CAGAACCGGG TCACAGATCA CAGAATAAAC 
AAGACGCTGC ATGATCTTGA AACTTTTATG CAAGGAGATT ATCTACTGGA TGAACTTGTA 
CAGTCATTGA AGGAATACGC CGATTATGAA TCTTTAGTAG AAATTATTTC CCAAAAAGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061914.3
CDS	131..1273
Translation	MRSRVLWGAARWLWPRRAVGPARRPLSSGSPPLEELFTRGGPLRTFLERQAGSEAHLKVRRPELLAVIKLLNEKERELRETEHLLHDENEDLRKLAENEITLCQKEITQLKHQIILLLVPSEETDENDLILEVTAGVGGQEAMLFTSEIFDMYQQYAAFKRWHFETLEYFPSELGGLRHASASIGGSEAYRHMKFEGGVHRVQRVPKTEKQGRVHTSTMTVAILPQPTEINLVINPKDLRIDTKRASGAGGQHVNTTDSAVRIVHLPTGVVSECQQERSQLKNKELAMTKLRAKLYSMHLEEEINKRQNARKIQIGSKGRSEKIRTYNFPQNRVTDHRINKTLHDLETFMQGDYLLDELVQSLKEYADYESLVEIISQKV

Target ORF information:

RefSeq Version	NM_019041.6
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019041.6

ORF Insert Sequence:

ATGCGGTCCC GGGTTCTGTG GGGCGCTGCC CGGTGGCTCT GGCCCCGCCG GGCCGTTGGC 
CCAGCCCGCC GGCCCCTGAG CTCCGGTAGC CCGCCGCTGG AGGAGCTGTT CACCCGGGGC 
GGGCCCTTGC GGACCTTCCT CGAGCGCCAG GCGGGGTCTG AAGCCCATTT GAAGGTCAGG 
AGGCCCGAGT TGCTGGCGGT GATCAAACTG CTGAACGAGA AGGAGCGGGA GCTGCGGGAG 
ACTGAGCACT TGCTGCACGA TGAGAATGAA GATTTAAGGA AACTTGCAGA GAATGAAATC 
ACTTTGTGTC AAAAAGAAAT AACTCAGCTG AAGCATCAGA TTATCTTACT TTTGGTTCCC 
TCAGAAGAAA CAGATGAAAA TGATTTGATC CTGGAAGTAA CTGCAGGAGT TGGAGGTCAG 
GAGGCAATGT TGTTTACATC AGAGATATTT GATATGTATC AGCAATATGC TGCATTTAAA 
AGATGGCATT TTGAAACCCT GGAATATTTT CCAAGTGAAC TAGGTGGCCT TAGACATGCA 
TCTGCCAGCA TTGGGGGTTC AGAAGCCTAT AGGCACATGA AATTTGAAGG AGGTGTTCAC 
AGAGTACAAA GAGTGCCAAA GACAGAAAAG CAAGGCCGCG TCCATACTAG CACCATGACT 
GTAGCAATAT TACCCCAGCC TACTGAGATT AATCTGGTGA TTAATCCGAA AGATTTGAGA 
ATTGACACTA AGCGAGCCAG TGGAGCTGGG GGGCAGCATG TAAATACCAC GGACAGTGCT 
GTCCGGATAG TTCATCTTCC AACAGGTGTT GTTTCTGAAT GTCAACAAGA GAGATCTCAG 
CTGAAAAATA AAGAGCTGGC TATGACAAAG TTACGTGCAA AACTGTACAG CATGCATCTA 
GAAGAAGAAA TAAATAAAAG ACAGAATGCT AGAAAAATTC AGATTGGAAG TAAAGGAAGA 
TCAGAGAAAA TAAGAACATA TAATTTTCCA CAGAACCGGG TCACAGATCA CAGAATAAAC 
AAGACGCTGC ATGATCTTGA AACTTTTATG CAAGGAGATT ATCTACTGGA TGAACTTGTA 
CAGTCATTGA AGGAATACGC CGATTATGAA TCTTTAGTAG AAATTATTTC CCAAAAAGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTRF1L ( NM_001301871.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001288800.1 Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu40858
Clone ID Related Accession (Same CDS sequence)	XM_017010960.1 , XM_017010959.1 , NM_001301871.1 , NM_001301871.2
Accession Version	NM_001301871.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	peptide chain release factor 1-like, mitochondrial isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA152954.1, AK027684.1, BC011873.2, AK095240.1, AI434611.1, AI479199.1, BU853336.1 and BX105260.1. On Sep 25, 2014 this sequence version replaced XM_005267036.2. Transcript Variant: This variant (4) uses an alternate in-frame splice site in the 5' coding region and uses a downstream start codon compared to variant 1. This variant encodes isoform 4, which has a shorter N-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.164292.1, SRR1803613.198268.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTGTTTA CATCAGAGAT ATTTGATATG TATCAGCAAT ATGCTGCATT TAAAAGATGG 
CATTTTGAAA CCCTGGAATA TTTTCCAAGT GAACTAGGTG GCCTTAGACA TGCATCTGCC 
AGCATTGGGG GTTCAGAAGC CTATAGGCAC ATGAAATTTG AAGGAGGTGT TCACAGAGTA 
CAAAGAGTGC CAAAGACAGA AAAGCAAGGC CGCGTCCATA CTAGCACCAT GACTGTAGCA 
ATATTACCCC AGCCTACTGA GATTAATCTG GTGATTAATC CGAAAGATTT GAGAATTGAC 
ACTAAGCGAG CCAGTGGAGC TGGGGGGCAG CATGTAAATA CCACGGACAG TGCTGTCCGG 
ATAGTTCATC TTCCAACAGG TGTTGTTTCT GAATGTCAAC AAGAGAGATC TCAGCTGAAA 
AATAAAGAGC TGGCTATGAC AAAGTTACGT GCAAAACTGT ACAGCATGCA TCTAGAAGAA 
GAAATAAATA AAAGACAGAA TGCTAGAAAA ATTCAGATTG GAAGTAAAGG AAGATCAGAG 
AAAATAAGAA CATATAATTT TCCACAGAAC CGGGTCACAG ATCACAGAAT AAACAAGACG 
CTGCATGATC TTGAAACTTT TATGCAAGGA GATTATCTAC TGGATGAACT TGTACAGTCA 
TTGAAGGAAT ACGCCGATTA TGAATCTTTA GTAGAAATTA TTTCCCAAAA AGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288800.1
CDS	532..1248
Translation	MLFTSEIFDMYQQYAAFKRWHFETLEYFPSELGGLRHASASIGGSEAYRHMKFEGGVHRVQRVPKTEKQGRVHTSTMTVAILPQPTEINLVINPKDLRIDTKRASGAGGQHVNTTDSAVRIVHLPTGVVSECQQERSQLKNKELAMTKLRAKLYSMHLEEEINKRQNARKIQIGSKGRSEKIRTYNFPQNRVTDHRINKTLHDLETFMQGDYLLDELVQSLKEYADYESLVEIISQKV

Target ORF information:

RefSeq Version	NM_001301871.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301871.1

ORF Insert Sequence:

ATGTTGTTTA CATCAGAGAT ATTTGATATG TATCAGCAAT ATGCTGCATT TAAAAGATGG 
CATTTTGAAA CCCTGGAATA TTTTCCAAGT GAACTAGGTG GCCTTAGACA TGCATCTGCC 
AGCATTGGGG GTTCAGAAGC CTATAGGCAC ATGAAATTTG AAGGAGGTGT TCACAGAGTA 
CAAAGAGTGC CAAAGACAGA AAAGCAAGGC CGCGTCCATA CTAGCACCAT GACTGTAGCA 
ATATTACCCC AGCCTACTGA GATTAATCTG GTGATTAATC CGAAAGATTT GAGAATTGAC 
ACTAAGCGAG CCAGTGGAGC TGGGGGGCAG CATGTAAATA CCACGGACAG TGCTGTCCGG 
ATAGTTCATC TTCCAACAGG TGTTGTTTCT GAATGTCAAC AAGAGAGATC TCAGCTGAAA 
AATAAAGAGC TGGCTATGAC AAAGTTACGT GCAAAACTGT ACAGCATGCA TCTAGAAGAA 
GAAATAAATA AAAGACAGAA TGCTAGAAAA ATTCAGATTG GAAGTAAAGG AAGATCAGAG 
AAAATAAGAA CATATAATTT TCCACAGAAC CGGGTCACAG ATCACAGAAT AAACAAGACG 
CTGCATGATC TTGAAACTTT TATGCAAGGA GATTATCTAC TGGATGAACT TGTACAGTCA 
TTGAAGGAAT ACGCCGATTA TGAATCTTTA GTAGAAATTA TTTCCCAAAA AGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTRF1L ( NM_001301871.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001288800.1 Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu40858
Clone ID Related Accession (Same CDS sequence)	XM_017010960.1 , XM_017010959.1 , NM_001301871.1 , NM_001301871.2
Accession Version	NM_001301871.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	peptide chain release factor 1-like, mitochondrial isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA152954.1, AK027684.1, BC011873.2, AK095240.1, AI434611.1, AI479199.1, BU853336.1, BX105260.1 and AL080276.9. On May 31, 2019 this sequence version replaced NM_001301871.1. Transcript Variant: This variant (4) uses an alternate in-frame splice site in the 5' coding region and uses a downstream start codon compared to variant 1. This variant encodes isoform 4, which has a shorter N-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.123004.1, SRR3476690.1167935.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTGTTTA CATCAGAGAT ATTTGATATG TATCAGCAAT ATGCTGCATT TAAAAGATGG 
CATTTTGAAA CCCTGGAATA TTTTCCAAGT GAACTAGGTG GCCTTAGACA TGCATCTGCC 
AGCATTGGGG GTTCAGAAGC CTATAGGCAC ATGAAATTTG AAGGAGGTGT TCACAGAGTA 
CAAAGAGTGC CAAAGACAGA AAAGCAAGGC CGCGTCCATA CTAGCACCAT GACTGTAGCA 
ATATTACCCC AGCCTACTGA GATTAATCTG GTGATTAATC CGAAAGATTT GAGAATTGAC 
ACTAAGCGAG CCAGTGGAGC TGGGGGGCAG CATGTAAATA CCACGGACAG TGCTGTCCGG 
ATAGTTCATC TTCCAACAGG TGTTGTTTCT GAATGTCAAC AAGAGAGATC TCAGCTGAAA 
AATAAAGAGC TGGCTATGAC AAAGTTACGT GCAAAACTGT ACAGCATGCA TCTAGAAGAA 
GAAATAAATA AAAGACAGAA TGCTAGAAAA ATTCAGATTG GAAGTAAAGG AAGATCAGAG 
AAAATAAGAA CATATAATTT TCCACAGAAC CGGGTCACAG ATCACAGAAT AAACAAGACG 
CTGCATGATC TTGAAACTTT TATGCAAGGA GATTATCTAC TGGATGAACT TGTACAGTCA 
TTGAAGGAAT ACGCCGATTA TGAATCTTTA GTAGAAATTA TTTCCCAAAA AGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288800.1
CDS	426..1142
Translation	MLFTSEIFDMYQQYAAFKRWHFETLEYFPSELGGLRHASASIGGSEAYRHMKFEGGVHRVQRVPKTEKQGRVHTSTMTVAILPQPTEINLVINPKDLRIDTKRASGAGGQHVNTTDSAVRIVHLPTGVVSECQQERSQLKNKELAMTKLRAKLYSMHLEEEINKRQNARKIQIGSKGRSEKIRTYNFPQNRVTDHRINKTLHDLETFMQGDYLLDELVQSLKEYADYESLVEIISQKV

Target ORF information:

RefSeq Version	NM_001301871.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301871.2

ORF Insert Sequence:

ATGTTGTTTA CATCAGAGAT ATTTGATATG TATCAGCAAT ATGCTGCATT TAAAAGATGG 
CATTTTGAAA CCCTGGAATA TTTTCCAAGT GAACTAGGTG GCCTTAGACA TGCATCTGCC 
AGCATTGGGG GTTCAGAAGC CTATAGGCAC ATGAAATTTG AAGGAGGTGT TCACAGAGTA 
CAAAGAGTGC CAAAGACAGA AAAGCAAGGC CGCGTCCATA CTAGCACCAT GACTGTAGCA 
ATATTACCCC AGCCTACTGA GATTAATCTG GTGATTAATC CGAAAGATTT GAGAATTGAC 
ACTAAGCGAG CCAGTGGAGC TGGGGGGCAG CATGTAAATA CCACGGACAG TGCTGTCCGG 
ATAGTTCATC TTCCAACAGG TGTTGTTTCT GAATGTCAAC AAGAGAGATC TCAGCTGAAA 
AATAAAGAGC TGGCTATGAC AAAGTTACGT GCAAAACTGT ACAGCATGCA TCTAGAAGAA 
GAAATAAATA AAAGACAGAA TGCTAGAAAA ATTCAGATTG GAAGTAAAGG AAGATCAGAG 
AAAATAAGAA CATATAATTT TCCACAGAAC CGGGTCACAG ATCACAGAAT AAACAAGACG 
CTGCATGATC TTGAAACTTT TATGCAAGGA GATTATCTAC TGGATGAACT TGTACAGTCA 
TTGAAGGAAT ACGCCGATTA TGAATCTTTA GTAGAAATTA TTTCCCAAAA AGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTRF1L ( XM_017010960.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866449.1 Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu40858
Clone ID Related Accession (Same CDS sequence)	XM_017010960.1 , XM_017010959.1 , NM_001301871.1 , NM_001301871.2
Accession Version	XM_017010960.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	peptide chain release factor 1-like, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTTTA CATCAGAGAT ATTTGATATG TATCAGCAAT ATGCTGCATT TAAAAGATGG 
CATTTTGAAA CCCTGGAATA TTTTCCAAGT GAACTAGGTG GCCTTAGACA TGCATCTGCC 
AGCATTGGGG GTTCAGAAGC CTATAGGCAC ATGAAATTTG AAGGAGGTGT TCACAGAGTA 
CAAAGAGTGC CAAAGACAGA AAAGCAAGGC CGCGTCCATA CTAGCACCAT GACTGTAGCA 
ATATTACCCC AGCCTACTGA GATTAATCTG GTGATTAATC CGAAAGATTT GAGAATTGAC 
ACTAAGCGAG CCAGTGGAGC TGGGGGGCAG CATGTAAATA CCACGGACAG TGCTGTCCGG 
ATAGTTCATC TTCCAACAGG TGTTGTTTCT GAATGTCAAC AAGAGAGATC TCAGCTGAAA 
AATAAAGAGC TGGCTATGAC AAAGTTACGT GCAAAACTGT ACAGCATGCA TCTAGAAGAA 
GAAATAAATA AAAGACAGAA TGCTAGAAAA ATTCAGATTG GAAGTAAAGG AAGATCAGAG 
AAAATAAGAA CATATAATTT TCCACAGAAC CGGGTCACAG ATCACAGAAT AAACAAGACG 
CTGCATGATC TTGAAACTTT TATGCAAGGA GATTATCTAC TGGATGAACT TGTACAGTCA 
TTGAAGGAAT ACGCCGATTA TGAATCTTTA GTAGAAATTA TTTCCCAAAA AGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866449.1
CDS	573..1289
Translation	MLFTSEIFDMYQQYAAFKRWHFETLEYFPSELGGLRHASASIGGSEAYRHMKFEGGVHRVQRVPKTEKQGRVHTSTMTVAILPQPTEINLVINPKDLRIDTKRASGAGGQHVNTTDSAVRIVHLPTGVVSECQQERSQLKNKELAMTKLRAKLYSMHLEEEINKRQNARKIQIGSKGRSEKIRTYNFPQNRVTDHRINKTLHDLETFMQGDYLLDELVQSLKEYADYESLVEIISQKV

Target ORF information:

RefSeq Version	XM_017010960.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010960.1

ORF Insert Sequence:

ATGTTGTTTA CATCAGAGAT ATTTGATATG TATCAGCAAT ATGCTGCATT TAAAAGATGG 
CATTTTGAAA CCCTGGAATA TTTTCCAAGT GAACTAGGTG GCCTTAGACA TGCATCTGCC 
AGCATTGGGG GTTCAGAAGC CTATAGGCAC ATGAAATTTG AAGGAGGTGT TCACAGAGTA 
CAAAGAGTGC CAAAGACAGA AAAGCAAGGC CGCGTCCATA CTAGCACCAT GACTGTAGCA 
ATATTACCCC AGCCTACTGA GATTAATCTG GTGATTAATC CGAAAGATTT GAGAATTGAC 
ACTAAGCGAG CCAGTGGAGC TGGGGGGCAG CATGTAAATA CCACGGACAG TGCTGTCCGG 
ATAGTTCATC TTCCAACAGG TGTTGTTTCT GAATGTCAAC AAGAGAGATC TCAGCTGAAA 
AATAAAGAGC TGGCTATGAC AAAGTTACGT GCAAAACTGT ACAGCATGCA TCTAGAAGAA 
GAAATAAATA AAAGACAGAA TGCTAGAAAA ATTCAGATTG GAAGTAAAGG AAGATCAGAG 
AAAATAAGAA CATATAATTT TCCACAGAAC CGGGTCACAG ATCACAGAAT AAACAAGACG 
CTGCATGATC TTGAAACTTT TATGCAAGGA GATTATCTAC TGGATGAACT TGTACAGTCA 
TTGAAGGAAT ACGCCGATTA TGAATCTTTA GTAGAAATTA TTTCCCAAAA AGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTRF1L ( XM_017010959.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866448.1 Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant X2, mRNA.

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CloneID	OHu40858
Clone ID Related Accession (Same CDS sequence)	XM_017010960.1 , XM_017010959.1 , NM_001301871.1 , NM_001301871.2
Accession Version	XM_017010959.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	peptide chain release factor 1-like, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTTTA CATCAGAGAT ATTTGATATG TATCAGCAAT ATGCTGCATT TAAAAGATGG 
CATTTTGAAA CCCTGGAATA TTTTCCAAGT GAACTAGGTG GCCTTAGACA TGCATCTGCC 
AGCATTGGGG GTTCAGAAGC CTATAGGCAC ATGAAATTTG AAGGAGGTGT TCACAGAGTA 
CAAAGAGTGC CAAAGACAGA AAAGCAAGGC CGCGTCCATA CTAGCACCAT GACTGTAGCA 
ATATTACCCC AGCCTACTGA GATTAATCTG GTGATTAATC CGAAAGATTT GAGAATTGAC 
ACTAAGCGAG CCAGTGGAGC TGGGGGGCAG CATGTAAATA CCACGGACAG TGCTGTCCGG 
ATAGTTCATC TTCCAACAGG TGTTGTTTCT GAATGTCAAC AAGAGAGATC TCAGCTGAAA 
AATAAAGAGC TGGCTATGAC AAAGTTACGT GCAAAACTGT ACAGCATGCA TCTAGAAGAA 
GAAATAAATA AAAGACAGAA TGCTAGAAAA ATTCAGATTG GAAGTAAAGG AAGATCAGAG 
AAAATAAGAA CATATAATTT TCCACAGAAC CGGGTCACAG ATCACAGAAT AAACAAGACG 
CTGCATGATC TTGAAACTTT TATGCAAGGA GATTATCTAC TGGATGAACT TGTACAGTCA 
TTGAAGGAAT ACGCCGATTA TGAATCTTTA GTAGAAATTA TTTCCCAAAA AGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866448.1
CDS	5164..5880
Translation	MLFTSEIFDMYQQYAAFKRWHFETLEYFPSELGGLRHASASIGGSEAYRHMKFEGGVHRVQRVPKTEKQGRVHTSTMTVAILPQPTEINLVINPKDLRIDTKRASGAGGQHVNTTDSAVRIVHLPTGVVSECQQERSQLKNKELAMTKLRAKLYSMHLEEEINKRQNARKIQIGSKGRSEKIRTYNFPQNRVTDHRINKTLHDLETFMQGDYLLDELVQSLKEYADYESLVEIISQKV

Target ORF information:

RefSeq Version	XM_017010959.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010959.1

ORF Insert Sequence:

ATGTTGTTTA CATCAGAGAT ATTTGATATG TATCAGCAAT ATGCTGCATT TAAAAGATGG 
CATTTTGAAA CCCTGGAATA TTTTCCAAGT GAACTAGGTG GCCTTAGACA TGCATCTGCC 
AGCATTGGGG GTTCAGAAGC CTATAGGCAC ATGAAATTTG AAGGAGGTGT TCACAGAGTA 
CAAAGAGTGC CAAAGACAGA AAAGCAAGGC CGCGTCCATA CTAGCACCAT GACTGTAGCA 
ATATTACCCC AGCCTACTGA GATTAATCTG GTGATTAATC CGAAAGATTT GAGAATTGAC 
ACTAAGCGAG CCAGTGGAGC TGGGGGGCAG CATGTAAATA CCACGGACAG TGCTGTCCGG 
ATAGTTCATC TTCCAACAGG TGTTGTTTCT GAATGTCAAC AAGAGAGATC TCAGCTGAAA 
AATAAAGAGC TGGCTATGAC AAAGTTACGT GCAAAACTGT ACAGCATGCA TCTAGAAGAA 
GAAATAAATA AAAGACAGAA TGCTAGAAAA ATTCAGATTG GAAGTAAAGG AAGATCAGAG 
AAAATAAGAA CATATAATTT TCCACAGAAC CGGGTCACAG ATCACAGAAT AAACAAGACG 
CTGCATGATC TTGAAACTTT TATGCAAGGA GATTATCTAC TGGATGAACT TGTACAGTCA 
TTGAAGGAAT ACGCCGATTA TGAATCTTTA GTAGAAATTA TTTCCCAAAA AGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTRF1L ( NM_001301870.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001288799.1 Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu40857
Clone ID Related Accession (Same CDS sequence)	NM_001301870.1 , NM_001301870.2
Accession Version	NM_001301870.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	peptide chain release factor 1-like, mitochondrial isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA152954.1, AK297671.1, BC011873.2, AK095240.1, AI434611.1, AI479199.1, BU853336.1 and BX105260.1. On Sep 25, 2014 this sequence version replaced XM_005267035.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This variant encodes isoform 2, which is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.113474.1, AK297671.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGGTCCC GGGTTCTGTG GGGCGCTGCC CGGTGGCTCT GGCCCCGCCG GGCCGTTGGC 
CCAGCCCGCC GGCCCCTGAG CTCCGGTAGC CCGCCGCTGG AGGAGCTGTT CACCCGGGGC 
GGGCCCTTGC GGACCTTCCT CGAGCGCCAG GCGGGGTCTG AAGCCCATTT GAAGGTCAGG 
AGGCCCGAGT TGCTGGCGGT GATCAAACTG CTGAACGAGA AGGAGCGGGA GCTGCGGGAG 
ACTGAGCACT TGCTGCACGA TGAGAATGAA GATTTAAGGA AACTTGCAGA GAATGAAATC 
ACTTTGTGTC AAAAAGAAAT AACTCAGCTG AAGCATCAGA TTATCTTACT TTTGGTTCCC 
TCAGAAGAAA CAGATGAAAA TGATTTGATC CTGGAAGTAA CTGCAGGAGT TGGAGGTGGC 
CTTAGACATG CATCTGCCAG CATTGGGGGT TCAGAAGCCT ATAGGCACAT GAAATTTGAA 
GGAGGTGTTC ACAGAGTACA AAGAGTGCCA AAGACAGAAA AGCAAGGCCG CGTCCATACT 
AGCACCATGA CTGTAGCAAT ATTACCCCAG CCTACTGAGA TTAATCTGGT GATTAATCCG 
AAAGATTTGA GAATTGACAC TAAGCGAGCC AGTGGAGCTG GGGGGCAGCA TGTAAATACC 
ACGGACAGTG CTGTCCGGAT AGTTCATCTT CCAACAGGTG TTGTTTCTGA ATGTCAACAA 
GAGAGATCTC AGCTGAAAAA TAAAGAGCTG GCTATGACAA AGTTACGTGC AAAACTGTAC 
AGCATGCATC TAGAAGAAGA AATAAATAAA AGACAGAATG CTAGAAAAAT TCAGATTGGA 
AGTAAAGGAA GATCAGAGAA AATAAGAACA TATAATTTTC CACAGAACCG GGTCACAGAT 
CACAGAATAA ACAAGACGCT GCATGATCTT GAAACTTTTA TGCAAGGAGA TTATCTACTG 
GATGAACTTG TACAGTCATT GAAGGAATAC GCCGATTATG AATCTTTAGT AGAAATTATT 
TCCCAAAAAG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288799.1
CDS	131..1165
Translation	MRSRVLWGAARWLWPRRAVGPARRPLSSGSPPLEELFTRGGPLRTFLERQAGSEAHLKVRRPELLAVIKLLNEKERELRETEHLLHDENEDLRKLAENEITLCQKEITQLKHQIILLLVPSEETDENDLILEVTAGVGGGLRHASASIGGSEAYRHMKFEGGVHRVQRVPKTEKQGRVHTSTMTVAILPQPTEINLVINPKDLRIDTKRASGAGGQHVNTTDSAVRIVHLPTGVVSECQQERSQLKNKELAMTKLRAKLYSMHLEEEINKRQNARKIQIGSKGRSEKIRTYNFPQNRVTDHRINKTLHDLETFMQGDYLLDELVQSLKEYADYESLVEIISQKV

Target ORF information:

RefSeq Version	NM_001301870.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301870.1

ORF Insert Sequence:

ATGCGGTCCC GGGTTCTGTG GGGCGCTGCC CGGTGGCTCT GGCCCCGCCG GGCCGTTGGC 
CCAGCCCGCC GGCCCCTGAG CTCCGGTAGC CCGCCGCTGG AGGAGCTGTT CACCCGGGGC 
GGGCCCTTGC GGACCTTCCT CGAGCGCCAG GCGGGGTCTG AAGCCCATTT GAAGGTCAGG 
AGGCCCGAGT TGCTGGCGGT GATCAAACTG CTGAACGAGA AGGAGCGGGA GCTGCGGGAG 
ACTGAGCACT TGCTGCACGA TGAGAATGAA GATTTAAGGA AACTTGCAGA GAATGAAATC 
ACTTTGTGTC AAAAAGAAAT AACTCAGCTG AAGCATCAGA TTATCTTACT TTTGGTTCCC 
TCAGAAGAAA CAGATGAAAA TGATTTGATC CTGGAAGTAA CTGCAGGAGT TGGAGGTGGC 
CTTAGACATG CATCTGCCAG CATTGGGGGT TCAGAAGCCT ATAGGCACAT GAAATTTGAA 
GGAGGTGTTC ACAGAGTACA AAGAGTGCCA AAGACAGAAA AGCAAGGCCG CGTCCATACT 
AGCACCATGA CTGTAGCAAT ATTACCCCAG CCTACTGAGA TTAATCTGGT GATTAATCCG 
AAAGATTTGA GAATTGACAC TAAGCGAGCC AGTGGAGCTG GGGGGCAGCA TGTAAATACC 
ACGGACAGTG CTGTCCGGAT AGTTCATCTT CCAACAGGTG TTGTTTCTGA ATGTCAACAA 
GAGAGATCTC AGCTGAAAAA TAAAGAGCTG GCTATGACAA AGTTACGTGC AAAACTGTAC 
AGCATGCATC TAGAAGAAGA AATAAATAAA AGACAGAATG CTAGAAAAAT TCAGATTGGA 
AGTAAAGGAA GATCAGAGAA AATAAGAACA TATAATTTTC CACAGAACCG GGTCACAGAT 
CACAGAATAA ACAAGACGCT GCATGATCTT GAAACTTTTA TGCAAGGAGA TTATCTACTG 
GATGAACTTG TACAGTCATT GAAGGAATAC GCCGATTATG AATCTTTAGT AGAAATTATT 
TCCCAAAAAG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTRF1L ( NM_001301870.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001288799.1 Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu40857
Clone ID Related Accession (Same CDS sequence)	NM_001301870.1 , NM_001301870.2
Accession Version	NM_001301870.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	peptide chain release factor 1-like, mitochondrial isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA152954.1, AK297671.1, BC011873.2, AK095240.1, AI434611.1, AI479199.1, BU853336.1, BX105260.1 and AL080276.9. On May 31, 2019 this sequence version replaced NM_001301870.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This variant encodes isoform 2, which is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.113474.1, DRR138524.814761.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGGTCCC GGGTTCTGTG GGGCGCTGCC CGGTGGCTCT GGCCCCGCCG GGCCGTTGGC 
CCAGCCCGCC GGCCCCTGAG CTCCGGTAGC CCGCCGCTGG AGGAGCTGTT CACCCGGGGC 
GGGCCCTTGC GGACCTTCCT CGAGCGCCAG GCGGGGTCTG AAGCCCATTT GAAGGTCAGG 
AGGCCCGAGT TGCTGGCGGT GATCAAACTG CTGAACGAGA AGGAGCGGGA GCTGCGGGAG 
ACTGAGCACT TGCTGCACGA TGAGAATGAA GATTTAAGGA AACTTGCAGA GAATGAAATC 
ACTTTGTGTC AAAAAGAAAT AACTCAGCTG AAGCATCAGA TTATCTTACT TTTGGTTCCC 
TCAGAAGAAA CAGATGAAAA TGATTTGATC CTGGAAGTAA CTGCAGGAGT TGGAGGTGGC 
CTTAGACATG CATCTGCCAG CATTGGGGGT TCAGAAGCCT ATAGGCACAT GAAATTTGAA 
GGAGGTGTTC ACAGAGTACA AAGAGTGCCA AAGACAGAAA AGCAAGGCCG CGTCCATACT 
AGCACCATGA CTGTAGCAAT ATTACCCCAG CCTACTGAGA TTAATCTGGT GATTAATCCG 
AAAGATTTGA GAATTGACAC TAAGCGAGCC AGTGGAGCTG GGGGGCAGCA TGTAAATACC 
ACGGACAGTG CTGTCCGGAT AGTTCATCTT CCAACAGGTG TTGTTTCTGA ATGTCAACAA 
GAGAGATCTC AGCTGAAAAA TAAAGAGCTG GCTATGACAA AGTTACGTGC AAAACTGTAC 
AGCATGCATC TAGAAGAAGA AATAAATAAA AGACAGAATG CTAGAAAAAT TCAGATTGGA 
AGTAAAGGAA GATCAGAGAA AATAAGAACA TATAATTTTC CACAGAACCG GGTCACAGAT 
CACAGAATAA ACAAGACGCT GCATGATCTT GAAACTTTTA TGCAAGGAGA TTATCTACTG 
GATGAACTTG TACAGTCATT GAAGGAATAC GCCGATTATG AATCTTTAGT AGAAATTATT 
TCCCAAAAAG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288799.1
CDS	25..1059
Translation	MRSRVLWGAARWLWPRRAVGPARRPLSSGSPPLEELFTRGGPLRTFLERQAGSEAHLKVRRPELLAVIKLLNEKERELRETEHLLHDENEDLRKLAENEITLCQKEITQLKHQIILLLVPSEETDENDLILEVTAGVGGGLRHASASIGGSEAYRHMKFEGGVHRVQRVPKTEKQGRVHTSTMTVAILPQPTEINLVINPKDLRIDTKRASGAGGQHVNTTDSAVRIVHLPTGVVSECQQERSQLKNKELAMTKLRAKLYSMHLEEEINKRQNARKIQIGSKGRSEKIRTYNFPQNRVTDHRINKTLHDLETFMQGDYLLDELVQSLKEYADYESLVEIISQKV

Target ORF information:

RefSeq Version	NM_001301870.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301870.2

ORF Insert Sequence:

ATGCGGTCCC GGGTTCTGTG GGGCGCTGCC CGGTGGCTCT GGCCCCGCCG GGCCGTTGGC 
CCAGCCCGCC GGCCCCTGAG CTCCGGTAGC CCGCCGCTGG AGGAGCTGTT CACCCGGGGC 
GGGCCCTTGC GGACCTTCCT CGAGCGCCAG GCGGGGTCTG AAGCCCATTT GAAGGTCAGG 
AGGCCCGAGT TGCTGGCGGT GATCAAACTG CTGAACGAGA AGGAGCGGGA GCTGCGGGAG 
ACTGAGCACT TGCTGCACGA TGAGAATGAA GATTTAAGGA AACTTGCAGA GAATGAAATC 
ACTTTGTGTC AAAAAGAAAT AACTCAGCTG AAGCATCAGA TTATCTTACT TTTGGTTCCC 
TCAGAAGAAA CAGATGAAAA TGATTTGATC CTGGAAGTAA CTGCAGGAGT TGGAGGTGGC 
CTTAGACATG CATCTGCCAG CATTGGGGGT TCAGAAGCCT ATAGGCACAT GAAATTTGAA 
GGAGGTGTTC ACAGAGTACA AAGAGTGCCA AAGACAGAAA AGCAAGGCCG CGTCCATACT 
AGCACCATGA CTGTAGCAAT ATTACCCCAG CCTACTGAGA TTAATCTGGT GATTAATCCG 
AAAGATTTGA GAATTGACAC TAAGCGAGCC AGTGGAGCTG GGGGGCAGCA TGTAAATACC 
ACGGACAGTG CTGTCCGGAT AGTTCATCTT CCAACAGGTG TTGTTTCTGA ATGTCAACAA 
GAGAGATCTC AGCTGAAAAA TAAAGAGCTG GCTATGACAA AGTTACGTGC AAAACTGTAC 
AGCATGCATC TAGAAGAAGA AATAAATAAA AGACAGAATG CTAGAAAAAT TCAGATTGGA 
AGTAAAGGAA GATCAGAGAA AATAAGAACA TATAATTTTC CACAGAACCG GGTCACAGAT 
CACAGAATAA ACAAGACGCT GCATGATCTT GAAACTTTTA TGCAAGGAGA TTATCTACTG 
GATGAACTTG TACAGTCATT GAAGGAATAC GCCGATTATG AATCTTTAGT AGAAATTATT 
TCCCAAAAAG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTRF1L ( NM_001301047.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287976.1 Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu40859
Clone ID Related Accession (Same CDS sequence)	NM_001301047.3 , NM_001301047.2
Accession Version	NM_001301047.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	peptide chain release factor 1-like, mitochondrial isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA152954.1, BC105973.1, AK095240.1, AI434611.1, AI479199.1, BU853336.1, BX105260.1 and AL080276.9. On May 31, 2019 this sequence version replaced NM_001301047.2. Transcript Variant: This variant (5) uses an alternate in-frame splice site in the 5' coding region, and lacks an alternate exon in the 3' coding region, which results in a frameshift and an early stop codon, compared to variant 1. This variant encodes isoform 5, which is shorter and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.272834.1, BC105973.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGGTCCC GGGTTCTGTG GGGCGCTGCC CGGTGGCTCT GGCCCCGCCG GGCCGTTGGC 
CCAGCCCGCC GGCCCCTGAG CTCCGGTAGC CCGCCGCTGG AGGAGCTGTT CACCCGGGGC 
GGGCCCTTGC GGACCTTCCT CGAGCGCCAG GCGGGGTCTG AAGCCCATTT GAAGGTCAGG 
AGGCCCGAGT TGCTGGCGGT GATCAAACTG CTGAACGAGA AGGAGCGGGA GCTGCGGGAG 
ACTGAGCACT TGCTGCACGA TGAGAATGAA GATTTAAGGA AACTTGCAGA GAATGAAATC 
ACTTTGTGTC AAAAAGAAAT AACTCAGCTG AAGCATCAGA TTATCTTACT TTTGGTTCCC 
TCAGAAGAAA CAGATGAAAA TGATTTGATC CTGGAAGTAA CTGCAGGAGT TGGAGGTGGC 
CTTAGACATG CATCTGCCAG CATTGGGGGT TCAGAAGCCT ATAGGCACAT GAAATTTGAA 
GGAGGTGTTC ACAGAGTACA AAGAGTGCCA AAGACAGAAA AGCAAGGCCG CGTCCATACT 
AGCACCATGA CTGTAGCAAT ATTACCCCAG CCTACTGAGA TTAATCTGGT GATTAATCCG 
AAAGATTTGA GAATTGACAC TAAGCGAGCC AGTGGAGCTG GGGGGCAGCA TGTAAATACC 
ACGGACAGTG CTGTCCGGAT AGTTCATCTT CCAACAGATT GGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287976.1
CDS	25..732
Translation	MRSRVLWGAARWLWPRRAVGPARRPLSSGSPPLEELFTRGGPLRTFLERQAGSEAHLKVRRPELLAVIKLLNEKERELRETEHLLHDENEDLRKLAENEITLCQKEITQLKHQIILLLVPSEETDENDLILEVTAGVGGGLRHASASIGGSEAYRHMKFEGGVHRVQRVPKTEKQGRVHTSTMTVAILPQPTEINLVINPKDLRIDTKRASGAGGQHVNTTDSAVRIVHLPTDWK

Target ORF information:

RefSeq Version	NM_001301047.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301047.3

ORF Insert Sequence:

ATGCGGTCCC GGGTTCTGTG GGGCGCTGCC CGGTGGCTCT GGCCCCGCCG GGCCGTTGGC 
CCAGCCCGCC GGCCCCTGAG CTCCGGTAGC CCGCCGCTGG AGGAGCTGTT CACCCGGGGC 
GGGCCCTTGC GGACCTTCCT CGAGCGCCAG GCGGGGTCTG AAGCCCATTT GAAGGTCAGG 
AGGCCCGAGT TGCTGGCGGT GATCAAACTG CTGAACGAGA AGGAGCGGGA GCTGCGGGAG 
ACTGAGCACT TGCTGCACGA TGAGAATGAA GATTTAAGGA AACTTGCAGA GAATGAAATC 
ACTTTGTGTC AAAAAGAAAT AACTCAGCTG AAGCATCAGA TTATCTTACT TTTGGTTCCC 
TCAGAAGAAA CAGATGAAAA TGATTTGATC CTGGAAGTAA CTGCAGGAGT TGGAGGTGGC 
CTTAGACATG CATCTGCCAG CATTGGGGGT TCAGAAGCCT ATAGGCACAT GAAATTTGAA 
GGAGGTGTTC ACAGAGTACA AAGAGTGCCA AAGACAGAAA AGCAAGGCCG CGTCCATACT 
AGCACCATGA CTGTAGCAAT ATTACCCCAG CCTACTGAGA TTAATCTGGT GATTAATCCG 
AAAGATTTGA GAATTGACAC TAAGCGAGCC AGTGGAGCTG GGGGGCAGCA TGTAAATACC 
ACGGACAGTG CTGTCCGGAT AGTTCATCTT CCAACAGATT GGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTRF1L ( NM_001301047.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287976.1 Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu40859
Clone ID Related Accession (Same CDS sequence)	NM_001301047.3 , NM_001301047.2
Accession Version	NM_001301047.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	peptide chain release factor 1-like, mitochondrial isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA152954.1, BC105973.1, AK095240.1, AI434611.1, AI479199.1, BU853336.1 and BX105260.1. On Sep 25, 2014 this sequence version replaced NM_001301047.1. Transcript Variant: This variant (5) uses an alternate in-frame splice site in the 5' coding region, and lacks an alternate exon in the 3' coding region, which results in a frameshift and an early stop codon, compared to variant 1. This variant encodes isoform 5, which is shorter and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.277180.1, BC105973.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGGTCCC GGGTTCTGTG GGGCGCTGCC CGGTGGCTCT GGCCCCGCCG GGCCGTTGGC 
CCAGCCCGCC GGCCCCTGAG CTCCGGTAGC CCGCCGCTGG AGGAGCTGTT CACCCGGGGC 
GGGCCCTTGC GGACCTTCCT CGAGCGCCAG GCGGGGTCTG AAGCCCATTT GAAGGTCAGG 
AGGCCCGAGT TGCTGGCGGT GATCAAACTG CTGAACGAGA AGGAGCGGGA GCTGCGGGAG 
ACTGAGCACT TGCTGCACGA TGAGAATGAA GATTTAAGGA AACTTGCAGA GAATGAAATC 
ACTTTGTGTC AAAAAGAAAT AACTCAGCTG AAGCATCAGA TTATCTTACT TTTGGTTCCC 
TCAGAAGAAA CAGATGAAAA TGATTTGATC CTGGAAGTAA CTGCAGGAGT TGGAGGTGGC 
CTTAGACATG CATCTGCCAG CATTGGGGGT TCAGAAGCCT ATAGGCACAT GAAATTTGAA 
GGAGGTGTTC ACAGAGTACA AAGAGTGCCA AAGACAGAAA AGCAAGGCCG CGTCCATACT 
AGCACCATGA CTGTAGCAAT ATTACCCCAG CCTACTGAGA TTAATCTGGT GATTAATCCG 
AAAGATTTGA GAATTGACAC TAAGCGAGCC AGTGGAGCTG GGGGGCAGCA TGTAAATACC 
ACGGACAGTG CTGTCCGGAT AGTTCATCTT CCAACAGATT GGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287976.1
CDS	131..838
Translation	MRSRVLWGAARWLWPRRAVGPARRPLSSGSPPLEELFTRGGPLRTFLERQAGSEAHLKVRRPELLAVIKLLNEKERELRETEHLLHDENEDLRKLAENEITLCQKEITQLKHQIILLLVPSEETDENDLILEVTAGVGGGLRHASASIGGSEAYRHMKFEGGVHRVQRVPKTEKQGRVHTSTMTVAILPQPTEINLVINPKDLRIDTKRASGAGGQHVNTTDSAVRIVHLPTDWK

Target ORF information:

RefSeq Version	NM_001301047.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301047.2

ORF Insert Sequence:

ATGCGGTCCC GGGTTCTGTG GGGCGCTGCC CGGTGGCTCT GGCCCCGCCG GGCCGTTGGC 
CCAGCCCGCC GGCCCCTGAG CTCCGGTAGC CCGCCGCTGG AGGAGCTGTT CACCCGGGGC 
GGGCCCTTGC GGACCTTCCT CGAGCGCCAG GCGGGGTCTG AAGCCCATTT GAAGGTCAGG 
AGGCCCGAGT TGCTGGCGGT GATCAAACTG CTGAACGAGA AGGAGCGGGA GCTGCGGGAG 
ACTGAGCACT TGCTGCACGA TGAGAATGAA GATTTAAGGA AACTTGCAGA GAATGAAATC 
ACTTTGTGTC AAAAAGAAAT AACTCAGCTG AAGCATCAGA TTATCTTACT TTTGGTTCCC 
TCAGAAGAAA CAGATGAAAA TGATTTGATC CTGGAAGTAA CTGCAGGAGT TGGAGGTGGC 
CTTAGACATG CATCTGCCAG CATTGGGGGT TCAGAAGCCT ATAGGCACAT GAAATTTGAA 
GGAGGTGTTC ACAGAGTACA AAGAGTGCCA AAGACAGAAA AGCAAGGCCG CGTCCATACT 
AGCACCATGA CTGTAGCAAT ATTACCCCAG CCTACTGAGA TTAATCTGGT GATTAATCCG 
AAAGATTTGA GAATTGACAC TAAGCGAGCC AGTGGAGCTG GGGGGCAGCA TGTAAATACC 
ACGGACAGTG CTGTCCGGAT AGTTCATCTT CCAACAGATT GGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTRF1L ( NM_001301872.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001288801.1 Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 6, mRNA.

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CloneID	OHu68403
Clone ID Related Accession (Same CDS sequence)	NM_001301872.1
Accession Version	NM_001301872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	peptide chain release factor 1-like, mitochondrial isoform 6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA152954.1 and AI628793.1. Transcript Variant: This variant (6) lacks several exons and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. This variant encodes isoform 6 which is shorter and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AI628793.1, SRR1163657.182812.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGGTCCC GGGTTCTGTG GGGCGCTGCC CGGTGGCTCT GGCCCCGCCG GGCCGTTGGC 
CCAGCCCGCC GGCCCCTGAG CTCCGGTAGC CCGCCGCTGG AGGAGCTGTT CACCCGGGGC 
GGGCCCTTGC GGACCTTCCT CGAGCGCCAG GCGGGGTCTG AAGCCCATTT GAAGGTCAGG 
AGGCCCGAGT TGCTGGCGGT GATCAAACTG CTGAACGAGA AGGAGCGGGA GCTGCGGGAG 
ACTGAGCACT TGCTGCACGA TGAGAATGAA GATTTAAGGA AACTTGCAGA GAATGAAATC 
ACTTTGTGTC AAAAAGAAAT AACTCAGCTG AAGCATCAGG TATGGTATTT CTGCAAAGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288801.1
CDS	131..493
Translation	MRSRVLWGAARWLWPRRAVGPARRPLSSGSPPLEELFTRGGPLRTFLERQAGSEAHLKVRRPELLAVIKLLNEKERELRETEHLLHDENEDLRKLAENEITLCQKEITQLKHQVWYFCKE

Target ORF information:

RefSeq Version	NM_001301872.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial translational release factor 1 like (MTRF1L), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301872.1

ORF Insert Sequence:

ATGCGGTCCC GGGTTCTGTG GGGCGCTGCC CGGTGGCTCT GGCCCCGCCG GGCCGTTGGC 
CCAGCCCGCC GGCCCCTGAG CTCCGGTAGC CCGCCGCTGG AGGAGCTGTT CACCCGGGGC 
GGGCCCTTGC GGACCTTCCT CGAGCGCCAG GCGGGGTCTG AAGCCCATTT GAAGGTCAGG 
AGGCCCGAGT TGCTGGCGGT GATCAAACTG CTGAACGAGA AGGAGCGGGA GCTGCGGGAG 
ACTGAGCACT TGCTGCACGA TGAGAATGAA GATTTAAGGA AACTTGCAGA GAATGAAATC 
ACTTTGTGTC AAAAAGAAAT AACTCAGCTG AAGCATCAGG TATGGTATTT CTGCAAAGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of 23 new prostate cancer susceptibility loci using the iCOGS custom genotyping array.
Nature genetics45(4)385-91, 391e1-2(2013 Apr)
Eeles RA,Olama AA,Benlloch S,Saunders EJ,Leongamornlert DA,Tymrakiewicz M,Ghoussaini M,Luccarini C,Dennis J,Jugurnauth-Little S,Dadaev T,Neal DE,Hamdy FC,Donovan JL,Muir K,Giles GG,Severi G,Wiklund F,Gronberg H,Haiman CA,Schumacher F,Henderson BE,Le Marchand L,Lindstrom S,Kraft P,Hunter DJ,Gapstur S,Chanock SJ,Berndt SI,Albanes D,Andriole G,Schleutker J,Weischer M,Canzian F,Riboli E,Key TJ,Travis RC,Campa D,Ingles SA,John EM,Hayes RB,Pharoah PD,Pashayan N,Khaw KT,Stanford JL,Ostrander EA,Signorello LB,Thibodeau SN,Schaid D,Maier C,Vogel W,Kibel AS,Cybulski C,Lubinski J,Cannon-Albright L,Brenner H,Park JY,Kaneva R,Batra J,Spurdle AB,Clements JA,Teixeira MR,Dicks E,Lee A,Dunning AM,Baynes C,Conroy D,Maranian MJ,Ahmed S,Govindasami K,Guy M,Wilkinson RA,Sawyer EJ,Morgan A,Dearnaley DP,Horwich A,Huddart RA,Khoo VS,Parker CC,Van As NJ,Woodhouse CJ,Thompson A,Dudderidge T,Ogden C,Cooper CS,Lophatananon A,Cox A,Southey MC,Hopper JL,English DR,Aly M,Adolfsson J,Xu J,Zheng SL,Yeager M,Kaaks R,Diver WR,Gaudet MM,Stern MC,Corral R,Joshi AD,Shahabi A,Wahlfors T,Tammela TL,Auvinen A,Virtamo J,Klarskov P,Nordestgaard BG,R?der MA,Nielsen SF,Bojesen SE,Siddiq A,Fitzgerald LM,Kolb S,Kwon EM,Karyadi DM,Blot WJ,Zheng W,Cai Q,McDonnell SK,Rinckleb AE,Drake B,Colditz G,Wokolorczyk D,Stephenson RA,Teerlink C,Muller H,Rothenbacher D,Sellers TA,Lin HY,Slavov C,Mitev V,Lose F,Srinivasan S,Maia S,Paulo P,Lange E,Cooney KA,Antoniou AC,Vincent D,Bacot F,Tessier DC,,,,,,Kote-Jarai Z,Easton DF

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

Mutations in C12orf65 in patients with encephalomyopathy and a mitochondrial translation defect.
American journal of human genetics87(1)115-22(2010 Jul)
Antonicka H,Ostergaard E,Sasarman F,Weraarpachai W,Wibrand F,Pedersen AM,Rodenburg RJ,van der Knaap MS,Smeitink JA,Chrzanowska-Lightowlers ZM,Shoubridge EA

Candidate gene/loci studies in cleft lip/palate and dental anomalies finds novel susceptibility genes for clefts.
Genetics in medicine : official journal of the American College of Medical Genetics10(9)668-74(2008 Sep)
Vieira AR,McHenry TG,Daack-Hirsch S,Murray JC,Marazita ML

The human mitochondrial translation release factor HMRF1L is methylated in the GGQ motif by the methyltransferase HMPrmC.
Biochemical and biophysical research communications373(1)99-103(2008 Aug)
Ishizawa T,Nozaki Y,Ueda T,Takeuchi N

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MTRF1L cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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見積依頼

Eメール

電話

FAX

オンラインフォーム