DDX4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX4
Entrez Gene ID	54514
Full Name	DEAD-box helicase 4
Synonyms	VASA
General protein information	Preferred Names DEAD-box helicase 4 Names probable ATP-dependent RNA helicase DDX4 DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 DEAD box protein 4 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4 vasa homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q11.2
Summary	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009].
Disorder MIM:	605281

mRNA and Protein(s)

mRNA	Protein	Name
XM_011543497.2	XP_011541799.1	probable ATP-dependent RNA helicase DDX4 isoform X3
NM_024415.3	NP_077726.1	probable ATP-dependent RNA helicase DDX4 isoform 1
NM_001166534.1	NP_001160006.1	probable ATP-dependent RNA helicase DDX4 isoform 4
NM_001142549.1	NP_001136021.1	probable ATP-dependent RNA helicase DDX4 isoform 2
NM_024415.2	NP_077726.1	probable ATP-dependent RNA helicase DDX4 isoform 1
NM_001166533.1	NP_001160005.1	probable ATP-dependent RNA helicase DDX4 isoform 3
XM_024446119.1	XP_024301887.1	probable ATP-dependent RNA helicase DDX4 isoform X1
XM_011543495.2	XP_011541797.1	probable ATP-dependent RNA helicase DDX4 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DDX4	XP_517757.3
Canis lupus familiaris (dog)	DDX4	XP_005617450.1
Gallus gallus (chicken)	DDX4	NP_990039.1
Homo sapiens (human)	DDX4	NP_077726.1
Bos taurus (cattle)	DDX4	NP_001007820.1
	DDX4	XP_001099856.2
Mus musculus (house mouse)	Ddx4	NP_001139357.1
Rattus norvegicus (Norway rat)	Ddx4	NP_001071115.1
Danio rerio (zebrafish)	vasa	NP_571132.1
Drosophila melanogaster (fruit fly)	vas	NP_001260458.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008578	XP_314684.3
Xenopus tropicalis (tropical clawed frog)	ddx4	NP_001016823.1

Gene Ontology
Bibliography

Related articles in PubMed

Does detection of DDX4 mRNA in cell-free seminal plasma represents a reliable noninvasive germ cell marker in patients with nonobstructive azoospermia?
Abdallah W, Hashad D, Abdelmaksoud R, Hashad MM
Andrologia49(8)(2017 Oct)

Germline factor DDX4 functions in blood-derived cancer cell phenotypes.
Schudrowitz N, Takagi S, Wessel GM, Yajima M
Cancer science108(8)1612-1619(2017 Aug)

Causes and Clinical Features of Infertile Men With Nonobstructive Azoospermia and Histopathologic Diagnosis of Hypospermatogenesis.
Cheng YS, Lu CW, Lin TY, Lin PY, Lin YM
Urology10562-68(2017 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results suggest that DDX4 positively regulates cell cycle progression of diverse somatic-derived blood cancer cells, implying its broad contributions to the cancer cell phenotype.
Title: Germline factor DDX4 functions in blood-derived cancer cell phenotypes.

The 20-mer peptide (residues 228-247) of human DDX-4, an ATP-dependent RNA helicase known to regulate germ cell development, binds to a unique shallow binding surface on RanBPM formed by highly conserved loops on the surface of the beta-sheet with two aspartates on one end, a positive patch on the opposite end, and a tryptophan lining at the bottom of the surface.
Title: Structural Basis for the Interaction between the IUS-SPRY Domain of RanBPM and DDX-4 in Germ Cell Development.

DDX4 cell-free seminal mRNA was absent in 44% of Sertoli cell-only syndrome cases diagnosed by testicular histopathology, but present in all patients presenting with maturation arrest or hypospermatogenesis
Title: Discrimination and characterization of Sertoli cell-only syndrome in non-obstructive azoospermia using cell-free seminal DDX4.

Data show that intrinsically disordered N terminus of DEAD-box RNA helicase Ddx4 forms organelles in HeLa cells and in vitro.
Title: Phase transition of a disordered nuage protein generates environmentally responsive membraneless organelles.

this study reports the first analysis and comparison of the expression patterns and colocalization of DDX4 and CD133 in 59 ovarian cancer patients via tissue microarray and immunofluorescence analysis to identify the expression patterns and relationship of those two markers in ovarian cancer cells.
Title: DDX4 (DEAD box polypeptide 4) colocalizes with cancer stem cell marker CD133 in ovarian cancers.

The following DDX4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31405	NM_024415.3 Latest version!	Homo sapiens DEAD-box helicase 4 (DDX4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu31405	XM_024446119.1 Latest version!	Homo sapiens DEAD-box helicase 4 (DDX4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu03884	NM_001166534.1 Latest version!	Homo sapiens DEAD-box helicase 4 (DDX4), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$335.30 $479.00
OHu03883	NM_001166533.1 Latest version!	Homo sapiens DEAD-box helicase 4 (DDX4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$496.30 $709.00
OHu32017	NM_001142549.1 Latest version!	Homo sapiens DEAD-box helicase 4 (DDX4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu68400	XM_011543495.2 Latest version!	Homo sapiens DEAD-box helicase 4 (DDX4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu68402	XM_011543497.2 Latest version!	Homo sapiens DEAD-box helicase 4 (DDX4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu31405	NM_024415.2	Homo sapiens DEAD-box helicase 4 (DDX4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX4 ( NM_024415.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_077726.1 Homo sapiens DEAD-box helicase 4 (DDX4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31405
Clone ID Related Accession (Same CDS sequence)	NM_024415.3 , NM_024415.2 , XM_024446119.1
Accession Version	NM_024415.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC088362.1, DB508256.1 and AC016632.7. On May 17, 2019 this sequence version replaced NM_024415.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292417.1, BC088362.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000505374.6/ ENSP00000424838.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGAGATG AAGATTGGGA AGCAGAAATC AACCCTCATA TGTCTTCCTA TGTTCCCATA 
TTTGAGAAGG ATAGGTATTC TGGAGAAAAT GGAGACAATT TTAACAGGAC TCCAGCTTCA 
TCATCAGAAA TGGATGATGG ACCTTCTCGA AGAGATCATT TCATGAAAAG TGGATTTGCC 
TCTGGGCGGA ATTTTGGAAA CAGAGATGCT GGTGAGTGTA ATAAGCGAGA TAATACATCC 
ACAATGGGTG GTTTTGGAGT TGGAAAGAGT TTTGGAAACA GAGGTTTTTC AAACAGCAGG 
TTTGAAGATG GTGATAGCTC TGGTTTCTGG AGAGAGTCTA GTAATGACTG CGAAGATAAT 
CCAACACGGA ACAGAGGGTT TTCCAAGAGA GGCGGCTATC GAGATGGAAA TAATTCAGAA 
GCTTCAGGGC CATACAGAAG AGGTGGAAGA GGTAGTTTCC GAGGTTGCCG TGGAGGATTT 
GGTCTAGGAA GTCCAAATAA TGACTTAGAC CCAGACGAAT GTATGCAGCG CACTGGTGGC 
CTTTTTGGTT CTAGAAGACC AGTATTAAGT GGCACAGGTA ATGGTGATAC TTCTCAAAGC 
AGAAGTGGCA GTGGAAGTGA ACGAGGTGGT TACAAAGGTT TAAATGAAGA AGTAATAACA 
GGCTCTGGAA AGAATTCTTG GAAGTCAGAA GCAGAAGGAG GAGAAAGTAG TGATACTCAA 
GGACCAAAAG TGACCTACAT ACCCCCTCCT CCACCTGAGG ATGAGGACTC CATCTTTGCA 
CATTATCAGA CAGGCATAAA CTTCGACAAA TACGACACTA TTCTTGTGGA AGTGTCTGGA 
CATGATGCAC CACCAGCAAT TCTGACTTTT GAAGAAGCTA ATCTCTGTCA GACACTGAAT 
AACAACATTG CTAAAGCTGG TTATACTAAG CTTACTCCTG TGCAAAAATA CAGTATTCCT 
ATCATACTTG CAGGACGAGA TTTGATGGCT TGCGCTCAAA CAGGGTCTGG GAAGACTGCG 
GCTTTTCTCC TACCAATTTT GGCTCATATG ATGCATGATG GAATAACTGC CAGTCGTTTT 
AAAGAGTTGC AGGAACCAGA GTGTATTATT GTAGCACCAA CTCGAGAATT GGTCAACCAG 
ATTTATTTGG AAGCCAGAAA ATTTTCTTTT GGGACTTGTG TAAGAGCTGT TGTTATATAT 
GGGGGAACCC AGCTGGGACA TTCAATTCGA CAAATAGTAC AAGGCTGTAA TATATTATGT 
GCTACTCCTG GAAGACTGAT GGATATCATA GGCAAAGAAA AGATTGGTCT CAAACAGATC 
AAATACTTAG TTTTGGATGA AGCTGATCGC ATGTTGGATA TGGGTTTTGG TCCAGAAATG 
AAGAAGTTAA TTTCTTGCCC AGGAATGCCA TCAAAGGAAC AGCGCCAAAC CCTTATGTTC 
AGTGCAACTT TTCCAGAGGA AATTCAAAGG TTGGCTGCAG AGTTTTTAAA GTCAAATTAT 
CTGTTTGTTG CTGTTGGACA AGTGGGTGGA GCATGTAGAG ATGTTCAGCA GACCGTTCTC 
CAAGTTGGCC AGTTCTCAAA AAGAGAAAAG CTCGTTGAAA TTCTGCGAAA CATAGGGGAT 
GAAAGAACTA TGGTCTTTGT TGAAACTAAG AAAAAAGCAG ATTTTATTGC AACTTTTCTT 
TGTCAAGAAA AAATATCAAC TACAAGTATT CATGGTGATC GGGAACAGAG AGAGCGGGAG 
CAAGCTCTTG GAGATTTTCG CTTTGGAAAG TGCCCAGTTC TTGTTGCTAC TTCAGTAGCT 
GCCAGAGGGC TGGATATTGA AAATGTGCAA CATGTTATCA ATTTTGATCT TCCTTCTACC 
ATTGATGAAT ATGTTCATCG AATTGGGCGT ACTGGTCGTT GTGGGAATAC TGGCAGAGCA 
ATTTCCTTTT TTGATCTTGA ATCGGATAAC CATTTAGCAC AGCCTCTAGT AAAAGTATTG 
ACAGATGCTC AACAGGATGT TCCTGCATGG TTGGAAGAAA TTGCCTTTAG TACATACATT 
CCTGGCTTCA GTGGTAGTAC AAGAGGAAAC GTGTTTGCAT CAGTTGATAC CAGAAAGGGC 
AAGAGCACTT TGAACACAGC TGGGTTTTCT TCTTCACAAG CTCCCAATCC AGTAGATGAT 
GAGTCATGGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077726.1
CDS	56..2230
Translation	MGDEDWEAEINPHMSSYVPIFEKDRYSGENGDNFNRTPASSSEMDDGPSRRDHFMKSGFASGRNFGNRDAGECNKRDNTSTMGGFGVGKSFGNRGFSNSRFEDGDSSGFWRESSNDCEDNPTRNRGFSKRGGYRDGNNSEASGPYRRGGRGSFRGCRGGFGLGSPNNDLDPDECMQRTGGLFGSRRPVLSGTGNGDTSQSRSGSGSERGGYKGLNEEVITGSGKNSWKSEAEGGESSDTQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHMMHDGITASRFKELQEPECIIVAPTRELVNQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLKQIKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAAEFLKSNYLFVAVGQVGGACRDVQQTVLQVGQFSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDTRKGKSTLNTAGFSSSQAPNPVDDESWD

Target ORF information:

RefSeq Version	NM_024415.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 4 (DDX4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024415.3

ORF Insert Sequence:

ATGGGAGATG AAGATTGGGA AGCAGAAATC AACCCTCATA TGTCTTCCTA TGTTCCCATA 
TTTGAGAAGG ATAGGTATTC TGGAGAAAAT GGAGACAATT TTAACAGGAC TCCAGCTTCA 
TCATCAGAAA TGGATGATGG ACCTTCTCGA AGAGATCATT TCATGAAAAG TGGATTTGCC 
TCTGGGCGGA ATTTTGGAAA CAGAGATGCT GGTGAGTGTA ATAAGCGAGA TAATACATCC 
ACAATGGGTG GTTTTGGAGT TGGAAAGAGT TTTGGAAACA GAGGTTTTTC AAACAGCAGG 
TTTGAAGATG GTGATAGCTC TGGTTTCTGG AGAGAGTCTA GTAATGACTG CGAAGATAAT 
CCAACACGGA ACAGAGGGTT TTCCAAGAGA GGCGGCTATC GAGATGGAAA TAATTCAGAA 
GCTTCAGGGC CATACAGAAG AGGTGGAAGA GGTAGTTTCC GAGGTTGCCG TGGAGGATTT 
GGTCTAGGAA GTCCAAATAA TGACTTAGAC CCAGACGAAT GTATGCAGCG CACTGGTGGC 
CTTTTTGGTT CTAGAAGACC AGTATTAAGT GGCACAGGTA ATGGTGATAC TTCTCAAAGC 
AGAAGTGGCA GTGGAAGTGA ACGAGGTGGT TACAAAGGTT TAAATGAAGA AGTAATAACA 
GGCTCTGGAA AGAATTCTTG GAAGTCAGAA GCAGAAGGAG GAGAAAGTAG TGATACTCAA 
GGACCAAAAG TGACCTACAT ACCCCCTCCT CCACCTGAGG ATGAGGACTC CATCTTTGCA 
CATTATCAGA CAGGCATAAA CTTCGACAAA TACGACACTA TTCTTGTGGA AGTGTCTGGA 
CATGATGCAC CACCAGCAAT TCTGACTTTT GAAGAAGCTA ATCTCTGTCA GACACTGAAT 
AACAACATTG CTAAAGCTGG TTATACTAAG CTTACTCCTG TGCAAAAATA CAGTATTCCT 
ATCATACTTG CAGGACGAGA TTTGATGGCT TGCGCTCAAA CAGGGTCTGG GAAGACTGCG 
GCTTTTCTCC TACCAATTTT GGCTCATATG ATGCATGATG GAATAACTGC CAGTCGTTTT 
AAAGAGTTGC AGGAACCAGA GTGTATTATT GTAGCACCAA CTCGAGAATT GGTCAACCAG 
ATTTATTTGG AAGCCAGAAA ATTTTCTTTT GGGACTTGTG TAAGAGCTGT TGTTATATAT 
GGGGGAACCC AGCTGGGACA TTCAATTCGA CAAATAGTAC AAGGCTGTAA TATATTATGT 
GCTACTCCTG GAAGACTGAT GGATATCATA GGCAAAGAAA AGATTGGTCT CAAACAGATC 
AAATACTTAG TTTTGGATGA AGCTGATCGC ATGTTGGATA TGGGTTTTGG TCCAGAAATG 
AAGAAGTTAA TTTCTTGCCC AGGAATGCCA TCAAAGGAAC AGCGCCAAAC CCTTATGTTC 
AGTGCAACTT TTCCAGAGGA AATTCAAAGG TTGGCTGCAG AGTTTTTAAA GTCAAATTAT 
CTGTTTGTTG CTGTTGGACA AGTGGGTGGA GCATGTAGAG ATGTTCAGCA GACCGTTCTC 
CAAGTTGGCC AGTTCTCAAA AAGAGAAAAG CTCGTTGAAA TTCTGCGAAA CATAGGGGAT 
GAAAGAACTA TGGTCTTTGT TGAAACTAAG AAAAAAGCAG ATTTTATTGC AACTTTTCTT 
TGTCAAGAAA AAATATCAAC TACAAGTATT CATGGTGATC GGGAACAGAG AGAGCGGGAG 
CAAGCTCTTG GAGATTTTCG CTTTGGAAAG TGCCCAGTTC TTGTTGCTAC TTCAGTAGCT 
GCCAGAGGGC TGGATATTGA AAATGTGCAA CATGTTATCA ATTTTGATCT TCCTTCTACC 
ATTGATGAAT ATGTTCATCG AATTGGGCGT ACTGGTCGTT GTGGGAATAC TGGCAGAGCA 
ATTTCCTTTT TTGATCTTGA ATCGGATAAC CATTTAGCAC AGCCTCTAGT AAAAGTATTG 
ACAGATGCTC AACAGGATGT TCCTGCATGG TTGGAAGAAA TTGCCTTTAG TACATACATT 
CCTGGCTTCA GTGGTAGTAC AAGAGGAAAC GTGTTTGCAT CAGTTGATAC CAGAAAGGGC 
AAGAGCACTT TGAACACAGC TGGGTTTTCT TCTTCACAAG CTCCCAATCC AGTAGATGAT 
GAGTCATGGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX4 ( NM_024415.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_077726.1 Homo sapiens DEAD-box helicase 4 (DDX4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31405
Clone ID Related Accession (Same CDS sequence)	NM_024415.3 , NM_024415.2 , XM_024446119.1
Accession Version	NM_024415.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB457419.1, BC088362.1 and DB508256.1. On or before Oct 28, 2009 this sequence version replaced NM_001136034.1, NM_024415.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292417.1, BC030638.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGAGATG AAGATTGGGA AGCAGAAATC AACCCTCATA TGTCTTCCTA TGTTCCCATA 
TTTGAGAAGG ATAGGTATTC TGGAGAAAAT GGAGACAATT TTAACAGGAC TCCAGCTTCA 
TCATCAGAAA TGGATGATGG ACCTTCTCGA AGAGATCATT TCATGAAAAG TGGATTTGCC 
TCTGGGCGGA ATTTTGGAAA CAGAGATGCT GGTGAGTGTA ATAAGCGAGA TAATACATCC 
ACAATGGGTG GTTTTGGAGT TGGAAAGAGT TTTGGAAACA GAGGTTTTTC AAACAGCAGG 
TTTGAAGATG GTGATAGCTC TGGTTTCTGG AGAGAGTCTA GTAATGACTG CGAAGATAAT 
CCAACACGGA ACAGAGGGTT TTCCAAGAGA GGCGGCTATC GAGATGGAAA TAATTCAGAA 
GCTTCAGGGC CATACAGAAG AGGTGGAAGA GGTAGTTTCC GAGGTTGCCG TGGAGGATTT 
GGTCTAGGAA GTCCAAATAA TGACTTAGAC CCAGACGAAT GTATGCAGCG CACTGGTGGC 
CTTTTTGGTT CTAGAAGACC AGTATTAAGT GGCACAGGTA ATGGTGATAC TTCTCAAAGC 
AGAAGTGGCA GTGGAAGTGA ACGAGGTGGT TACAAAGGTT TAAATGAAGA AGTAATAACA 
GGCTCTGGAA AGAATTCTTG GAAGTCAGAA GCAGAAGGAG GAGAAAGTAG TGATACTCAA 
GGACCAAAAG TGACCTACAT ACCCCCTCCT CCACCTGAGG ATGAGGACTC CATCTTTGCA 
CATTATCAGA CAGGCATAAA CTTCGACAAA TACGACACTA TTCTTGTGGA AGTGTCTGGA 
CATGATGCAC CACCAGCAAT TCTGACTTTT GAAGAAGCTA ATCTCTGTCA GACACTGAAT 
AACAACATTG CTAAAGCTGG TTATACTAAG CTTACTCCTG TGCAAAAATA CAGTATTCCT 
ATCATACTTG CAGGACGAGA TTTGATGGCT TGCGCTCAAA CAGGGTCTGG GAAGACTGCG 
GCTTTTCTCC TACCAATTTT GGCTCATATG ATGCATGATG GAATAACTGC CAGTCGTTTT 
AAAGAGTTGC AGGAACCAGA GTGTATTATT GTAGCACCAA CTCGAGAATT GGTCAACCAG 
ATTTATTTGG AAGCCAGAAA ATTTTCTTTT GGGACTTGTG TAAGAGCTGT TGTTATATAT 
GGGGGAACCC AGCTGGGACA TTCAATTCGA CAAATAGTAC AAGGCTGTAA TATATTATGT 
GCTACTCCTG GAAGACTGAT GGATATCATA GGCAAAGAAA AGATTGGTCT CAAACAGATC 
AAATACTTAG TTTTGGATGA AGCTGATCGC ATGTTGGATA TGGGTTTTGG TCCAGAAATG 
AAGAAGTTAA TTTCTTGCCC AGGAATGCCA TCAAAGGAAC AGCGCCAAAC CCTTATGTTC 
AGTGCAACTT TTCCAGAGGA AATTCAAAGG TTGGCTGCAG AGTTTTTAAA GTCAAATTAT 
CTGTTTGTTG CTGTTGGACA AGTGGGTGGA GCATGTAGAG ATGTTCAGCA GACCGTTCTC 
CAAGTTGGCC AGTTCTCAAA AAGAGAAAAG CTCGTTGAAA TTCTGCGAAA CATAGGGGAT 
GAAAGAACTA TGGTCTTTGT TGAAACTAAG AAAAAAGCAG ATTTTATTGC AACTTTTCTT 
TGTCAAGAAA AAATATCAAC TACAAGTATT CATGGTGATC GGGAACAGAG AGAGCGGGAG 
CAAGCTCTTG GAGATTTTCG CTTTGGAAAG TGCCCAGTTC TTGTTGCTAC TTCAGTAGCT 
GCCAGAGGGC TGGATATTGA AAATGTGCAA CATGTTATCA ATTTTGATCT TCCTTCTACC 
ATTGATGAAT ATGTTCATCG AATTGGGCGT ACTGGTCGTT GTGGGAATAC TGGCAGAGCA 
ATTTCCTTTT TTGATCTTGA ATCGGATAAC CATTTAGCAC AGCCTCTAGT AAAAGTATTG 
ACAGATGCTC AACAGGATGT TCCTGCATGG TTGGAAGAAA TTGCCTTTAG TACATACATT 
CCTGGCTTCA GTGGTAGTAC AAGAGGAAAC GTGTTTGCAT CAGTTGATAC CAGAAAGGGC 
AAGAGCACTT TGAACACAGC TGGGTTTTCT TCTTCACAAG CTCCCAATCC AGTAGATGAT 
GAGTCATGGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077726.1
CDS	100..2274
Translation	MGDEDWEAEINPHMSSYVPIFEKDRYSGENGDNFNRTPASSSEMDDGPSRRDHFMKSGFASGRNFGNRDAGECNKRDNTSTMGGFGVGKSFGNRGFSNSRFEDGDSSGFWRESSNDCEDNPTRNRGFSKRGGYRDGNNSEASGPYRRGGRGSFRGCRGGFGLGSPNNDLDPDECMQRTGGLFGSRRPVLSGTGNGDTSQSRSGSGSERGGYKGLNEEVITGSGKNSWKSEAEGGESSDTQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHMMHDGITASRFKELQEPECIIVAPTRELVNQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLKQIKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAAEFLKSNYLFVAVGQVGGACRDVQQTVLQVGQFSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDTRKGKSTLNTAGFSSSQAPNPVDDESWD

Target ORF information:

RefSeq Version	NM_024415.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 4 (DDX4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024415.2

ORF Insert Sequence:

ATGGGAGATG AAGATTGGGA AGCAGAAATC AACCCTCATA TGTCTTCCTA TGTTCCCATA 
TTTGAGAAGG ATAGGTATTC TGGAGAAAAT GGAGACAATT TTAACAGGAC TCCAGCTTCA 
TCATCAGAAA TGGATGATGG ACCTTCTCGA AGAGATCATT TCATGAAAAG TGGATTTGCC 
TCTGGGCGGA ATTTTGGAAA CAGAGATGCT GGTGAGTGTA ATAAGCGAGA TAATACATCC 
ACAATGGGTG GTTTTGGAGT TGGAAAGAGT TTTGGAAACA GAGGTTTTTC AAACAGCAGG 
TTTGAAGATG GTGATAGCTC TGGTTTCTGG AGAGAGTCTA GTAATGACTG CGAAGATAAT 
CCAACACGGA ACAGAGGGTT TTCCAAGAGA GGCGGCTATC GAGATGGAAA TAATTCAGAA 
GCTTCAGGGC CATACAGAAG AGGTGGAAGA GGTAGTTTCC GAGGTTGCCG TGGAGGATTT 
GGTCTAGGAA GTCCAAATAA TGACTTAGAC CCAGACGAAT GTATGCAGCG CACTGGTGGC 
CTTTTTGGTT CTAGAAGACC AGTATTAAGT GGCACAGGTA ATGGTGATAC TTCTCAAAGC 
AGAAGTGGCA GTGGAAGTGA ACGAGGTGGT TACAAAGGTT TAAATGAAGA AGTAATAACA 
GGCTCTGGAA AGAATTCTTG GAAGTCAGAA GCAGAAGGAG GAGAAAGTAG TGATACTCAA 
GGACCAAAAG TGACCTACAT ACCCCCTCCT CCACCTGAGG ATGAGGACTC CATCTTTGCA 
CATTATCAGA CAGGCATAAA CTTCGACAAA TACGACACTA TTCTTGTGGA AGTGTCTGGA 
CATGATGCAC CACCAGCAAT TCTGACTTTT GAAGAAGCTA ATCTCTGTCA GACACTGAAT 
AACAACATTG CTAAAGCTGG TTATACTAAG CTTACTCCTG TGCAAAAATA CAGTATTCCT 
ATCATACTTG CAGGACGAGA TTTGATGGCT TGCGCTCAAA CAGGGTCTGG GAAGACTGCG 
GCTTTTCTCC TACCAATTTT GGCTCATATG ATGCATGATG GAATAACTGC CAGTCGTTTT 
AAAGAGTTGC AGGAACCAGA GTGTATTATT GTAGCACCAA CTCGAGAATT GGTCAACCAG 
ATTTATTTGG AAGCCAGAAA ATTTTCTTTT GGGACTTGTG TAAGAGCTGT TGTTATATAT 
GGGGGAACCC AGCTGGGACA TTCAATTCGA CAAATAGTAC AAGGCTGTAA TATATTATGT 
GCTACTCCTG GAAGACTGAT GGATATCATA GGCAAAGAAA AGATTGGTCT CAAACAGATC 
AAATACTTAG TTTTGGATGA AGCTGATCGC ATGTTGGATA TGGGTTTTGG TCCAGAAATG 
AAGAAGTTAA TTTCTTGCCC AGGAATGCCA TCAAAGGAAC AGCGCCAAAC CCTTATGTTC 
AGTGCAACTT TTCCAGAGGA AATTCAAAGG TTGGCTGCAG AGTTTTTAAA GTCAAATTAT 
CTGTTTGTTG CTGTTGGACA AGTGGGTGGA GCATGTAGAG ATGTTCAGCA GACCGTTCTC 
CAAGTTGGCC AGTTCTCAAA AAGAGAAAAG CTCGTTGAAA TTCTGCGAAA CATAGGGGAT 
GAAAGAACTA TGGTCTTTGT TGAAACTAAG AAAAAAGCAG ATTTTATTGC AACTTTTCTT 
TGTCAAGAAA AAATATCAAC TACAAGTATT CATGGTGATC GGGAACAGAG AGAGCGGGAG 
CAAGCTCTTG GAGATTTTCG CTTTGGAAAG TGCCCAGTTC TTGTTGCTAC TTCAGTAGCT 
GCCAGAGGGC TGGATATTGA AAATGTGCAA CATGTTATCA ATTTTGATCT TCCTTCTACC 
ATTGATGAAT ATGTTCATCG AATTGGGCGT ACTGGTCGTT GTGGGAATAC TGGCAGAGCA 
ATTTCCTTTT TTGATCTTGA ATCGGATAAC CATTTAGCAC AGCCTCTAGT AAAAGTATTG 
ACAGATGCTC AACAGGATGT TCCTGCATGG TTGGAAGAAA TTGCCTTTAG TACATACATT 
CCTGGCTTCA GTGGTAGTAC AAGAGGAAAC GTGTTTGCAT CAGTTGATAC CAGAAAGGGC 
AAGAGCACTT TGAACACAGC TGGGTTTTCT TCTTCACAAG CTCCCAATCC AGTAGATGAT 
GAGTCATGGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX4 ( XM_024446119.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024301887.1 Homo sapiens DEAD-box helicase 4 (DDX4), transcript variant X1, mRNA.

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CloneID	OHu31405
Clone ID Related Accession (Same CDS sequence)	NM_024415.3 , NM_024415.2 , XM_024446119.1
Accession Version	XM_024446119.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG AAGATTGGGA AGCAGAAATC AACCCTCATA TGTCTTCCTA TGTTCCCATA 
TTTGAGAAGG ATAGGTATTC TGGAGAAAAT GGAGACAATT TTAACAGGAC TCCAGCTTCA 
TCATCAGAAA TGGATGATGG ACCTTCTCGA AGAGATCATT TCATGAAAAG TGGATTTGCC 
TCTGGGCGGA ATTTTGGAAA CAGAGATGCT GGTGAGTGTA ATAAGCGAGA TAATACATCC 
ACAATGGGTG GTTTTGGAGT TGGAAAGAGT TTTGGAAACA GAGGTTTTTC AAACAGCAGG 
TTTGAAGATG GTGATAGCTC TGGTTTCTGG AGAGAGTCTA GTAATGACTG CGAAGATAAT 
CCAACACGGA ACAGAGGGTT TTCCAAGAGA GGCGGCTATC GAGATGGAAA TAATTCAGAA 
GCTTCAGGGC CATACAGAAG AGGTGGAAGA GGTAGTTTCC GAGGTTGCCG TGGAGGATTT 
GGTCTAGGAA GTCCAAATAA TGACTTAGAC CCAGACGAAT GTATGCAGCG CACTGGTGGC 
CTTTTTGGTT CTAGAAGACC AGTATTAAGT GGCACAGGTA ATGGTGATAC TTCTCAAAGC 
AGAAGTGGCA GTGGAAGTGA ACGAGGTGGT TACAAAGGTT TAAATGAAGA AGTAATAACA 
GGCTCTGGAA AGAATTCTTG GAAGTCAGAA GCAGAAGGAG GAGAAAGTAG TGATACTCAA 
GGACCAAAAG TGACCTACAT ACCCCCTCCT CCACCTGAGG ATGAGGACTC CATCTTTGCA 
CATTATCAGA CAGGCATAAA CTTCGACAAA TACGACACTA TTCTTGTGGA AGTGTCTGGA 
CATGATGCAC CACCAGCAAT TCTGACTTTT GAAGAAGCTA ATCTCTGTCA GACACTGAAT 
AACAACATTG CTAAAGCTGG TTATACTAAG CTTACTCCTG TGCAAAAATA CAGTATTCCT 
ATCATACTTG CAGGACGAGA TTTGATGGCT TGCGCTCAAA CAGGGTCTGG GAAGACTGCG 
GCTTTTCTCC TACCAATTTT GGCTCATATG ATGCATGATG GAATAACTGC CAGTCGTTTT 
AAAGAGTTGC AGGAACCAGA GTGTATTATT GTAGCACCAA CTCGAGAATT GGTCAACCAG 
ATTTATTTGG AAGCCAGAAA ATTTTCTTTT GGGACTTGTG TAAGAGCTGT TGTTATATAT 
GGGGGAACCC AGCTGGGACA TTCAATTCGA CAAATAGTAC AAGGCTGTAA TATATTATGT 
GCTACTCCTG GAAGACTGAT GGATATCATA GGCAAAGAAA AGATTGGTCT CAAACAGATC 
AAATACTTAG TTTTGGATGA AGCTGATCGC ATGTTGGATA TGGGTTTTGG TCCAGAAATG 
AAGAAGTTAA TTTCTTGCCC AGGAATGCCA TCAAAGGAAC AGCGCCAAAC CCTTATGTTC 
AGTGCAACTT TTCCAGAGGA AATTCAAAGG TTGGCTGCAG AGTTTTTAAA GTCAAATTAT 
CTGTTTGTTG CTGTTGGACA AGTGGGTGGA GCATGTAGAG ATGTTCAGCA GACCGTTCTC 
CAAGTTGGCC AGTTCTCAAA AAGAGAAAAG CTCGTTGAAA TTCTGCGAAA CATAGGGGAT 
GAAAGAACTA TGGTCTTTGT TGAAACTAAG AAAAAAGCAG ATTTTATTGC AACTTTTCTT 
TGTCAAGAAA AAATATCAAC TACAAGTATT CATGGTGATC GGGAACAGAG AGAGCGGGAG 
CAAGCTCTTG GAGATTTTCG CTTTGGAAAG TGCCCAGTTC TTGTTGCTAC TTCAGTAGCT 
GCCAGAGGGC TGGATATTGA AAATGTGCAA CATGTTATCA ATTTTGATCT TCCTTCTACC 
ATTGATGAAT ATGTTCATCG AATTGGGCGT ACTGGTCGTT GTGGGAATAC TGGCAGAGCA 
ATTTCCTTTT TTGATCTTGA ATCGGATAAC CATTTAGCAC AGCCTCTAGT AAAAGTATTG 
ACAGATGCTC AACAGGATGT TCCTGCATGG TTGGAAGAAA TTGCCTTTAG TACATACATT 
CCTGGCTTCA GTGGTAGTAC AAGAGGAAAC GTGTTTGCAT CAGTTGATAC CAGAAAGGGC 
AAGAGCACTT TGAACACAGC TGGGTTTTCT TCTTCACAAG CTCCCAATCC AGTAGATGAT 
GAGTCATGGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024301887.1
CDS	251..2425
Translation	MGDEDWEAEINPHMSSYVPIFEKDRYSGENGDNFNRTPASSSEMDDGPSRRDHFMKSGFASGRNFGNRDAGECNKRDNTSTMGGFGVGKSFGNRGFSNSRFEDGDSSGFWRESSNDCEDNPTRNRGFSKRGGYRDGNNSEASGPYRRGGRGSFRGCRGGFGLGSPNNDLDPDECMQRTGGLFGSRRPVLSGTGNGDTSQSRSGSGSERGGYKGLNEEVITGSGKNSWKSEAEGGESSDTQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHMMHDGITASRFKELQEPECIIVAPTRELVNQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLKQIKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAAEFLKSNYLFVAVGQVGGACRDVQQTVLQVGQFSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDTRKGKSTLNTAGFSSSQAPNPVDDESWD

Target ORF information:

RefSeq Version	XM_024446119.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 4 (DDX4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024446119.1

ORF Insert Sequence:

ATGGGAGATG AAGATTGGGA AGCAGAAATC AACCCTCATA TGTCTTCCTA TGTTCCCATA 
TTTGAGAAGG ATAGGTATTC TGGAGAAAAT GGAGACAATT TTAACAGGAC TCCAGCTTCA 
TCATCAGAAA TGGATGATGG ACCTTCTCGA AGAGATCATT TCATGAAAAG TGGATTTGCC 
TCTGGGCGGA ATTTTGGAAA CAGAGATGCT GGTGAGTGTA ATAAGCGAGA TAATACATCC 
ACAATGGGTG GTTTTGGAGT TGGAAAGAGT TTTGGAAACA GAGGTTTTTC AAACAGCAGG 
TTTGAAGATG GTGATAGCTC TGGTTTCTGG AGAGAGTCTA GTAATGACTG CGAAGATAAT 
CCAACACGGA ACAGAGGGTT TTCCAAGAGA GGCGGCTATC GAGATGGAAA TAATTCAGAA 
GCTTCAGGGC CATACAGAAG AGGTGGAAGA GGTAGTTTCC GAGGTTGCCG TGGAGGATTT 
GGTCTAGGAA GTCCAAATAA TGACTTAGAC CCAGACGAAT GTATGCAGCG CACTGGTGGC 
CTTTTTGGTT CTAGAAGACC AGTATTAAGT GGCACAGGTA ATGGTGATAC TTCTCAAAGC 
AGAAGTGGCA GTGGAAGTGA ACGAGGTGGT TACAAAGGTT TAAATGAAGA AGTAATAACA 
GGCTCTGGAA AGAATTCTTG GAAGTCAGAA GCAGAAGGAG GAGAAAGTAG TGATACTCAA 
GGACCAAAAG TGACCTACAT ACCCCCTCCT CCACCTGAGG ATGAGGACTC CATCTTTGCA 
CATTATCAGA CAGGCATAAA CTTCGACAAA TACGACACTA TTCTTGTGGA AGTGTCTGGA 
CATGATGCAC CACCAGCAAT TCTGACTTTT GAAGAAGCTA ATCTCTGTCA GACACTGAAT 
AACAACATTG CTAAAGCTGG TTATACTAAG CTTACTCCTG TGCAAAAATA CAGTATTCCT 
ATCATACTTG CAGGACGAGA TTTGATGGCT TGCGCTCAAA CAGGGTCTGG GAAGACTGCG 
GCTTTTCTCC TACCAATTTT GGCTCATATG ATGCATGATG GAATAACTGC CAGTCGTTTT 
AAAGAGTTGC AGGAACCAGA GTGTATTATT GTAGCACCAA CTCGAGAATT GGTCAACCAG 
ATTTATTTGG AAGCCAGAAA ATTTTCTTTT GGGACTTGTG TAAGAGCTGT TGTTATATAT 
GGGGGAACCC AGCTGGGACA TTCAATTCGA CAAATAGTAC AAGGCTGTAA TATATTATGT 
GCTACTCCTG GAAGACTGAT GGATATCATA GGCAAAGAAA AGATTGGTCT CAAACAGATC 
AAATACTTAG TTTTGGATGA AGCTGATCGC ATGTTGGATA TGGGTTTTGG TCCAGAAATG 
AAGAAGTTAA TTTCTTGCCC AGGAATGCCA TCAAAGGAAC AGCGCCAAAC CCTTATGTTC 
AGTGCAACTT TTCCAGAGGA AATTCAAAGG TTGGCTGCAG AGTTTTTAAA GTCAAATTAT 
CTGTTTGTTG CTGTTGGACA AGTGGGTGGA GCATGTAGAG ATGTTCAGCA GACCGTTCTC 
CAAGTTGGCC AGTTCTCAAA AAGAGAAAAG CTCGTTGAAA TTCTGCGAAA CATAGGGGAT 
GAAAGAACTA TGGTCTTTGT TGAAACTAAG AAAAAAGCAG ATTTTATTGC AACTTTTCTT 
TGTCAAGAAA AAATATCAAC TACAAGTATT CATGGTGATC GGGAACAGAG AGAGCGGGAG 
CAAGCTCTTG GAGATTTTCG CTTTGGAAAG TGCCCAGTTC TTGTTGCTAC TTCAGTAGCT 
GCCAGAGGGC TGGATATTGA AAATGTGCAA CATGTTATCA ATTTTGATCT TCCTTCTACC 
ATTGATGAAT ATGTTCATCG AATTGGGCGT ACTGGTCGTT GTGGGAATAC TGGCAGAGCA 
ATTTCCTTTT TTGATCTTGA ATCGGATAAC CATTTAGCAC AGCCTCTAGT AAAAGTATTG 
ACAGATGCTC AACAGGATGT TCCTGCATGG TTGGAAGAAA TTGCCTTTAG TACATACATT 
CCTGGCTTCA GTGGTAGTAC AAGAGGAAAC GTGTTTGCAT CAGTTGATAC CAGAAAGGGC 
AAGAGCACTT TGAACACAGC TGGGTTTTCT TCTTCACAAG CTCCCAATCC AGTAGATGAT 
GAGTCATGGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX4 ( NM_001166534.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001160006.1 Homo sapiens DEAD-box helicase 4 (DDX4), transcript variant 4, mRNA.

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CloneID	OHu03884
Clone ID Related Accession (Same CDS sequence)	NM_001166534.1
Accession Version	NM_001166534.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX4 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB051674.1, AK093439.1, DB022413.1 and DB508256.1. Transcript Variant: This variant (4) has a novel first exon representing use of an alternate internal promoter site compared to variant 1. The predicted protein isoform (4) is shorter and has a distinct N-terminus. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK093439.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2151119 [ECO:0000348] ##Evidence-Data-END##

ATGGGGTCTA GGAATTTATT TTTAACCAAC TCTCCAGAGT CTAGTAATGA CTGCGAAGAT 
AATCCAACAC GGAACAGAGG GTTTTCCAAG AGAGGCGATA ATGACTTAGA CCCAGACGAA 
TGTATGCAGC GCACTGGTGG CCTTTTTGGT TCTAGAAGAC CAGTATTAAG TGGCACAGGT 
AATGGTGATA CTTCTCAAAG CAGAAGTGGC AGTGGAAGTG AACGAGATTC TTGGAAGTCA 
GAAGCAGAAG GAGGAGAAAG TAGTGATACT CAAGGACCAA AAGTGACCTA CATACCCCCT 
CCTCCACCTG AGGATGAGGA CTCCATCTTT GCACATTATC AGACAGGCAT AAACTTCGAC 
AAATACGACA CTATTCTTGT GGAAGTGTCT GGACATGATG CACCACCAGC AATTCTGACT 
TTTGAAGAAG CTAATCTCTG TCAGACACTG AATAACAACA TTGCTAAAGC TGGTTATACT 
AAGCTTACTC CTGTGCAAAA ATACAGTATT CCTATCATAC TTGCAGGACG AGATTTGATG 
GCTTGCGCTC AAACAGGGTC TGGGAAGACT GCGGCTTTTC TCCTACCAAT TTTGGCTCAT 
ATGATGCATG ATGGAATAAC TGCCAGTCGT TTTAAAGAGT TGCAGGAACC AGAGTGTATT 
ATTGTAGCAC CAACTCGAGA ATTGGTCAAC CAGATTTATT TGGAAGCCAG AAAATTTTCT 
TTTGGGACTT GTGTAAGAGC TGTTGTTATA TATGGGGGAA CCCAGCTGGG ACATTCAATT 
CGACAAATAG TACAAGGCTG TAATATATTA TGTGCTACTC CTGGAAGACT GATGGATATC 
ATAGGCAAAG AAAAGATTGG TCTCAAACAG ATCAAATACT TAGTTTTGGA TGAAGCTGAT 
CGCATGTTGG ATATGGGTTT TGGTCCAGAA ATGAAGAAGT TAATTTCTTG CCCAGGAATG 
CCATCAAAGG AACAGCGCCA AACCCTTATG TTCAGTGCAA CTTTTCCAGA GGAAATTCAA 
AGGTTGGCTG CAGAGTTTTT AAAGTCAAAT TATCTGTTTG TTGCTGTTGG ACAAGTGGGT 
GGAGCATGTA GAGATGTTCA GCAGACCGTT CTCCAAGTTG GCCAGTTCTC AAAAAGAGAA 
AAGCTCGTTG AAATTCTGCG AAACATAGGG GATGAAAGAA CTATGGTCTT TGTTGAAACT 
AAGAAAAAAG CAGATTTTAT TGCAACTTTT CTTTGTCAAG AAAAAATATC AACTACAAGT 
ATTCATGGTG ATCGGGAACA GAGAGAGCGG GAGCAAGCTC TTGGAGATTT TCGCTTTGGA 
AAGTGCCCAG TTCTTGTTGC TACTTCAGTA GCTGCCAGAG GGCTGGATAT TGAAAATGTG 
CAACATGTTA TCAATTTTGA TCTTCCTTCT ACCATTGATG AATATGTTCA TCGAATTGGG 
CGTACTGGTC GTTGTGGGAA TACTGGCAGA GCAATTTCCT TTTTTGATCT TGAATCGGAT 
AACCATTTAG CACAGCCTCT AGTAAAAGTA TTGACAGATG CTCAACAGGA TGTTCCTGCA 
TGGTTGGAAG AAATTGCCTT TAGTACATAC ATTCCTGGCT TCAGTGGTAG TACAAGAGGA 
AACGTGTTTG CATCAGTTGA TACCAGAAAG GGCAAGAGCA CTTTGAACAC AGCTGGGTTT 
TCTTCTTCAC AAGCTCCCAA TCCAGTAGAT GATGAGTCAT GGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001160006.1
CDS	46..1773
Translation	MGSRNLFLTNSPESSNDCEDNPTRNRGFSKRGDNDLDPDECMQRTGGLFGSRRPVLSGTGNGDTSQSRSGSGSERDSWKSEAEGGESSDTQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHMMHDGITASRFKELQEPECIIVAPTRELVNQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLKQIKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAAEFLKSNYLFVAVGQVGGACRDVQQTVLQVGQFSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDTRKGKSTLNTAGFSSSQAPNPVDDESWD

Target ORF information:

RefSeq Version	NM_001166534.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 4 (DDX4), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166534.1

ORF Insert Sequence:

ATGGGGTCTA GGAATTTATT TTTAACCAAC TCTCCAGAGT CTAGTAATGA CTGCGAAGAT 
AATCCAACAC GGAACAGAGG GTTTTCCAAG AGAGGCGATA ATGACTTAGA CCCAGACGAA 
TGTATGCAGC GCACTGGTGG CCTTTTTGGT TCTAGAAGAC CAGTATTAAG TGGCACAGGT 
AATGGTGATA CTTCTCAAAG CAGAAGTGGC AGTGGAAGTG AACGAGATTC TTGGAAGTCA 
GAAGCAGAAG GAGGAGAAAG TAGTGATACT CAAGGACCAA AAGTGACCTA CATACCCCCT 
CCTCCACCTG AGGATGAGGA CTCCATCTTT GCACATTATC AGACAGGCAT AAACTTCGAC 
AAATACGACA CTATTCTTGT GGAAGTGTCT GGACATGATG CACCACCAGC AATTCTGACT 
TTTGAAGAAG CTAATCTCTG TCAGACACTG AATAACAACA TTGCTAAAGC TGGTTATACT 
AAGCTTACTC CTGTGCAAAA ATACAGTATT CCTATCATAC TTGCAGGACG AGATTTGATG 
GCTTGCGCTC AAACAGGGTC TGGGAAGACT GCGGCTTTTC TCCTACCAAT TTTGGCTCAT 
ATGATGCATG ATGGAATAAC TGCCAGTCGT TTTAAAGAGT TGCAGGAACC AGAGTGTATT 
ATTGTAGCAC CAACTCGAGA ATTGGTCAAC CAGATTTATT TGGAAGCCAG AAAATTTTCT 
TTTGGGACTT GTGTAAGAGC TGTTGTTATA TATGGGGGAA CCCAGCTGGG ACATTCAATT 
CGACAAATAG TACAAGGCTG TAATATATTA TGTGCTACTC CTGGAAGACT GATGGATATC 
ATAGGCAAAG AAAAGATTGG TCTCAAACAG ATCAAATACT TAGTTTTGGA TGAAGCTGAT 
CGCATGTTGG ATATGGGTTT TGGTCCAGAA ATGAAGAAGT TAATTTCTTG CCCAGGAATG 
CCATCAAAGG AACAGCGCCA AACCCTTATG TTCAGTGCAA CTTTTCCAGA GGAAATTCAA 
AGGTTGGCTG CAGAGTTTTT AAAGTCAAAT TATCTGTTTG TTGCTGTTGG ACAAGTGGGT 
GGAGCATGTA GAGATGTTCA GCAGACCGTT CTCCAAGTTG GCCAGTTCTC AAAAAGAGAA 
AAGCTCGTTG AAATTCTGCG AAACATAGGG GATGAAAGAA CTATGGTCTT TGTTGAAACT 
AAGAAAAAAG CAGATTTTAT TGCAACTTTT CTTTGTCAAG AAAAAATATC AACTACAAGT 
ATTCATGGTG ATCGGGAACA GAGAGAGCGG GAGCAAGCTC TTGGAGATTT TCGCTTTGGA 
AAGTGCCCAG TTCTTGTTGC TACTTCAGTA GCTGCCAGAG GGCTGGATAT TGAAAATGTG 
CAACATGTTA TCAATTTTGA TCTTCCTTCT ACCATTGATG AATATGTTCA TCGAATTGGG 
CGTACTGGTC GTTGTGGGAA TACTGGCAGA GCAATTTCCT TTTTTGATCT TGAATCGGAT 
AACCATTTAG CACAGCCTCT AGTAAAAGTA TTGACAGATG CTCAACAGGA TGTTCCTGCA 
TGGTTGGAAG AAATTGCCTT TAGTACATAC ATTCCTGGCT TCAGTGGTAG TACAAGAGGA 
AACGTGTTTG CATCAGTTGA TACCAGAAAG GGCAAGAGCA CTTTGAACAC AGCTGGGTTT 
TCTTCTTCAC AAGCTCCCAA TCCAGTAGAT GATGAGTCAT GGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX4 ( NM_001166533.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001160005.1 Homo sapiens DEAD-box helicase 4 (DDX4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu03883
Clone ID Related Accession (Same CDS sequence)	NM_001166533.1
Accession Version	NM_001166533.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX4 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB457419.1, BC030638.1, BI559479.1 and BC088362.1. Transcript Variant: This variant (3) lacks an in-frame coding exon compared to variant 1 resulting in a shorter protein isoform (3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END##

ATGGGAGATG AAGATTGGGA AGCAGAAATC AACCCTCATA TGTCTTCCTA TGTTCCCATA 
TTTGAGAAGG ATAGGTATTC TGGAGAAAAT GGAGACAATT TTAACAGGAC TCCAGCTTCA 
TCATCAGAAA TGGATGATGG ACCTTCTCGA AGAGATCATT TCATGAAAAG TGGATTTGCC 
TCTGGGCGGA ATTTTGGAAA CAGAGATGCT GGTGAGTGTA ATAAGCGAGA TAATACATCC 
ACAATGGGTG GTTTTGGAGT TGGAAAGAGT TTTGGAAACA GAGGTTTTTC AAACAGCAGG 
TTTGAAGATG GTGATAGCTC TGGTTTCTGG AGAGGCTATC GAGATGGAAA TAATTCAGAA 
GCTTCAGGGC CATACAGAAG AGGTGGAAGA GGTAGTTTCC GAGGTTGCCG TGGAGGATTT 
GGTCTAGGAA GTCCAAATAA TGACTTAGAC CCAGACGAAT GTATGCAGCG CACTGGTGGC 
CTTTTTGGTT CTAGAAGACC AGTATTAAGT GGCACAGGTA ATGGTGATAC TTCTCAAAGC 
AGAAGTGGCA GTGGAAGTGA ACGAGGTGGT TACAAAGGTT TAAATGAAGA AGTAATAACA 
GGCTCTGGAA AGAATTCTTG GAAGTCAGAA GCAGAAGGAG GAGAAAGTAG TGATACTCAA 
GGACCAAAAG TGACCTACAT ACCCCCTCCT CCACCTGAGG ATGAGGACTC CATCTTTGCA 
CATTATCAGA CAGGCATAAA CTTCGACAAA TACGACACTA TTCTTGTGGA AGTGTCTGGA 
CATGATGCAC CACCAGCAAT TCTGACTTTT GAAGAAGCTA ATCTCTGTCA GACACTGAAT 
AACAACATTG CTAAAGCTGG TTATACTAAG CTTACTCCTG TGCAAAAATA CAGTATTCCT 
ATCATACTTG CAGGACGAGA TTTGATGGCT TGCGCTCAAA CAGGGTCTGG GAAGACTGCG 
GCTTTTCTCC TACCAATTTT GGCTCATATG ATGCATGATG GAATAACTGC CAGTCGTTTT 
AAAGAGTTGC AGGAACCAGA GTGTATTATT GTAGCACCAA CTCGAGAATT GGTCAACCAG 
ATTTATTTGG AAGCCAGAAA ATTTTCTTTT GGGACTTGTG TAAGAGCTGT TGTTATATAT 
GGGGGAACCC AGCTGGGACA TTCAATTCGA CAAATAGTAC AAGGCTGTAA TATATTATGT 
GCTACTCCTG GAAGACTGAT GGATATCATA GGCAAAGAAA AGATTGGTCT CAAACAGATC 
AAATACTTAG TTTTGGATGA AGCTGATCGC ATGTTGGATA TGGGTTTTGG TCCAGAAATG 
AAGAAGTTAA TTTCTTGCCC AGGAATGCCA TCAAAGGAAC AGCGCCAAAC CCTTATGTTC 
AGTGCAACTT TTCCAGAGGA AATTCAAAGG TTGGCTGCAG AGTTTTTAAA GTCAAATTAT 
CTGTTTGTTG CTGTTGGACA AGTGGGTGGA GCATGTAGAG ATGTTCAGCA GACCGTTCTC 
CAAGTTGGCC AGTTCTCAAA AAGAGAAAAG CTCGTTGAAA TTCTGCGAAA CATAGGGGAT 
GAAAGAACTA TGGTCTTTGT TGAAACTAAG AAAAAAGCAG ATTTTATTGC AACTTTTCTT 
TGTCAAGAAA AAATATCAAC TACAAGTATT CATGGTGATC GGGAACAGAG AGAGCGGGAG 
CAAGCTCTTG GAGATTTTCG CTTTGGAAAG TGCCCAGTTC TTGTTGCTAC TTCAGTAGCT 
GCCAGAGGGC TGGATATTGA AAATGTGCAA CATGTTATCA ATTTTGATCT TCCTTCTACC 
ATTGATGAAT ATGTTCATCG AATTGGGCGT ACTGGTCGTT GTGGGAATAC TGGCAGAGCA 
ATTTCCTTTT TTGATCTTGA ATCGGATAAC CATTTAGCAC AGCCTCTAGT AAAAGTATTG 
ACAGATGCTC AACAGGATGT TCCTGCATGG TTGGAAGAAA TTGCCTTTAG TACATACATT 
CCTGGCTTCA GTGGTAGTAC AAGAGGAAAC GTGTTTGCAT CAGTTGATAC CAGAAAGGGC 
AAGAGCACTT TGAACACAGC TGGGTTTTCT TCTTCACAAG CTCCCAATCC AGTAGATGAT 
GAGTCATGGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001160005.1
CDS	100..2214
Translation	MGDEDWEAEINPHMSSYVPIFEKDRYSGENGDNFNRTPASSSEMDDGPSRRDHFMKSGFASGRNFGNRDAGECNKRDNTSTMGGFGVGKSFGNRGFSNSRFEDGDSSGFWRGYRDGNNSEASGPYRRGGRGSFRGCRGGFGLGSPNNDLDPDECMQRTGGLFGSRRPVLSGTGNGDTSQSRSGSGSERGGYKGLNEEVITGSGKNSWKSEAEGGESSDTQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHMMHDGITASRFKELQEPECIIVAPTRELVNQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLKQIKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAAEFLKSNYLFVAVGQVGGACRDVQQTVLQVGQFSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDTRKGKSTLNTAGFSSSQAPNPVDDESWD

Target ORF information:

RefSeq Version	NM_001166533.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 4 (DDX4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166533.1

ORF Insert Sequence:

ATGGGAGATG AAGATTGGGA AGCAGAAATC AACCCTCATA TGTCTTCCTA TGTTCCCATA 
TTTGAGAAGG ATAGGTATTC TGGAGAAAAT GGAGACAATT TTAACAGGAC TCCAGCTTCA 
TCATCAGAAA TGGATGATGG ACCTTCTCGA AGAGATCATT TCATGAAAAG TGGATTTGCC 
TCTGGGCGGA ATTTTGGAAA CAGAGATGCT GGTGAGTGTA ATAAGCGAGA TAATACATCC 
ACAATGGGTG GTTTTGGAGT TGGAAAGAGT TTTGGAAACA GAGGTTTTTC AAACAGCAGG 
TTTGAAGATG GTGATAGCTC TGGTTTCTGG AGAGGCTATC GAGATGGAAA TAATTCAGAA 
GCTTCAGGGC CATACAGAAG AGGTGGAAGA GGTAGTTTCC GAGGTTGCCG TGGAGGATTT 
GGTCTAGGAA GTCCAAATAA TGACTTAGAC CCAGACGAAT GTATGCAGCG CACTGGTGGC 
CTTTTTGGTT CTAGAAGACC AGTATTAAGT GGCACAGGTA ATGGTGATAC TTCTCAAAGC 
AGAAGTGGCA GTGGAAGTGA ACGAGGTGGT TACAAAGGTT TAAATGAAGA AGTAATAACA 
GGCTCTGGAA AGAATTCTTG GAAGTCAGAA GCAGAAGGAG GAGAAAGTAG TGATACTCAA 
GGACCAAAAG TGACCTACAT ACCCCCTCCT CCACCTGAGG ATGAGGACTC CATCTTTGCA 
CATTATCAGA CAGGCATAAA CTTCGACAAA TACGACACTA TTCTTGTGGA AGTGTCTGGA 
CATGATGCAC CACCAGCAAT TCTGACTTTT GAAGAAGCTA ATCTCTGTCA GACACTGAAT 
AACAACATTG CTAAAGCTGG TTATACTAAG CTTACTCCTG TGCAAAAATA CAGTATTCCT 
ATCATACTTG CAGGACGAGA TTTGATGGCT TGCGCTCAAA CAGGGTCTGG GAAGACTGCG 
GCTTTTCTCC TACCAATTTT GGCTCATATG ATGCATGATG GAATAACTGC CAGTCGTTTT 
AAAGAGTTGC AGGAACCAGA GTGTATTATT GTAGCACCAA CTCGAGAATT GGTCAACCAG 
ATTTATTTGG AAGCCAGAAA ATTTTCTTTT GGGACTTGTG TAAGAGCTGT TGTTATATAT 
GGGGGAACCC AGCTGGGACA TTCAATTCGA CAAATAGTAC AAGGCTGTAA TATATTATGT 
GCTACTCCTG GAAGACTGAT GGATATCATA GGCAAAGAAA AGATTGGTCT CAAACAGATC 
AAATACTTAG TTTTGGATGA AGCTGATCGC ATGTTGGATA TGGGTTTTGG TCCAGAAATG 
AAGAAGTTAA TTTCTTGCCC AGGAATGCCA TCAAAGGAAC AGCGCCAAAC CCTTATGTTC 
AGTGCAACTT TTCCAGAGGA AATTCAAAGG TTGGCTGCAG AGTTTTTAAA GTCAAATTAT 
CTGTTTGTTG CTGTTGGACA AGTGGGTGGA GCATGTAGAG ATGTTCAGCA GACCGTTCTC 
CAAGTTGGCC AGTTCTCAAA AAGAGAAAAG CTCGTTGAAA TTCTGCGAAA CATAGGGGAT 
GAAAGAACTA TGGTCTTTGT TGAAACTAAG AAAAAAGCAG ATTTTATTGC AACTTTTCTT 
TGTCAAGAAA AAATATCAAC TACAAGTATT CATGGTGATC GGGAACAGAG AGAGCGGGAG 
CAAGCTCTTG GAGATTTTCG CTTTGGAAAG TGCCCAGTTC TTGTTGCTAC TTCAGTAGCT 
GCCAGAGGGC TGGATATTGA AAATGTGCAA CATGTTATCA ATTTTGATCT TCCTTCTACC 
ATTGATGAAT ATGTTCATCG AATTGGGCGT ACTGGTCGTT GTGGGAATAC TGGCAGAGCA 
ATTTCCTTTT TTGATCTTGA ATCGGATAAC CATTTAGCAC AGCCTCTAGT AAAAGTATTG 
ACAGATGCTC AACAGGATGT TCCTGCATGG TTGGAAGAAA TTGCCTTTAG TACATACATT 
CCTGGCTTCA GTGGTAGTAC AAGAGGAAAC GTGTTTGCAT CAGTTGATAC CAGAAAGGGC 
AAGAGCACTT TGAACACAGC TGGGTTTTCT TCTTCACAAG CTCCCAATCC AGTAGATGAT 
GAGTCATGGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX4 ( NM_001142549.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001136021.1 Homo sapiens DEAD-box helicase 4 (DDX4), transcript variant 2, mRNA.

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CloneID	OHu32017
Clone ID Related Accession (Same CDS sequence)	NM_001142549.1
Accession Version	NM_001142549.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB457419.1, BC047455.1, AL137462.1 and DB508256.1. Transcript Variant: This variant (2) lacks an in-frame coding exon, as compared to variant 1. The resulting isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC047455.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END##

ATGGGAGATG AAGATTGGGA AGCAGAAATC AACCCTCATA TGTCTTCCTA TGTTCCCATA 
TTTGAGAAGG ATAGGTATTC TGGAGAAAAT GGAGACAATT TTAACAGGAC TCCAGCTTCA 
TCATCAGAAA TGGATGATGG ACCTTCTCGA AGAGATCATT TCATGAAAAG TGGATTTGCC 
TCTGGGCGGA ATTTTGGAAA CAGAGATGCT GGTGAGTGTA ATAAGCGAGA TAATACATCC 
ACAATGGGTG GTTTTGGAGT TGGAAAGAGT TTTGGAAACA GAGGTTTTTC AAACAGCAGG 
TTTGAAGATG GTGATAGCTC TGGTTTCTGG AGAGAGTCTA GTAATGACTG CGAAGATAAT 
CCAACACGGA ACAGAGGGTT TTCCAAGAGA GGCGATAATG ACTTAGACCC AGACGAATGT 
ATGCAGCGCA CTGGTGGCCT TTTTGGTTCT AGAAGACCAG TATTAAGTGG CACAGGTAAT 
GGTGATACTT CTCAAAGCAG AAGTGGCAGT GGAAGTGAAC GAGGTGGTTA CAAAGGTTTA 
AATGAAGAAG TAATAACAGG CTCTGGAAAG AATTCTTGGA AGTCAGAAGC AGAAGGAGGA 
GAAAGTAGTG ATACTCAAGG ACCAAAAGTG ACCTACATAC CCCCTCCTCC ACCTGAGGAT 
GAGGACTCCA TCTTTGCACA TTATCAGACA GGCATAAACT TCGACAAATA CGACACTATT 
CTTGTGGAAG TGTCTGGACA TGATGCACCA CCAGCAATTC TGACTTTTGA AGAAGCTAAT 
CTCTGTCAGA CACTGAATAA CAACATTGCT AAAGCTGGTT ATACTAAGCT TACTCCTGTG 
CAAAAATACA GTATTCCTAT CATACTTGCA GGACGAGATT TGATGGCTTG CGCTCAAACA 
GGGTCTGGGA AGACTGCGGC TTTTCTCCTA CCAATTTTGG CTCATATGAT GCATGATGGA 
ATAACTGCCA GTCGTTTTAA AGAGTTGCAG GAACCAGAGT GTATTATTGT AGCACCAACT 
CGAGAATTGG TCAACCAGAT TTATTTGGAA GCCAGAAAAT TTTCTTTTGG GACTTGTGTA 
AGAGCTGTTG TTATATATGG GGGAACCCAG CTGGGACATT CAATTCGACA AATAGTACAA 
GGCTGTAATA TATTATGTGC TACTCCTGGA AGACTGATGG ATATCATAGG CAAAGAAAAG 
ATTGGTCTCA AACAGATCAA ATACTTAGTT TTGGATGAAG CTGATCGCAT GTTGGATATG 
GGTTTTGGTC CAGAAATGAA GAAGTTAATT TCTTGCCCAG GAATGCCATC AAAGGAACAG 
CGCCAAACCC TTATGTTCAG TGCAACTTTT CCAGAGGAAA TTCAAAGGTT GGCTGCAGAG 
TTTTTAAAGT CAAATTATCT GTTTGTTGCT GTTGGACAAG TGGGTGGAGC ATGTAGAGAT 
GTTCAGCAGA CCGTTCTCCA AGTTGGCCAG TTCTCAAAAA GAGAAAAGCT CGTTGAAATT 
CTGCGAAACA TAGGGGATGA AAGAACTATG GTCTTTGTTG AAACTAAGAA AAAAGCAGAT 
TTTATTGCAA CTTTTCTTTG TCAAGAAAAA ATATCAACTA CAAGTATTCA TGGTGATCGG 
GAACAGAGAG AGCGGGAGCA AGCTCTTGGA GATTTTCGCT TTGGAAAGTG CCCAGTTCTT 
GTTGCTACTT CAGTAGCTGC CAGAGGGCTG GATATTGAAA ATGTGCAACA TGTTATCAAT 
TTTGATCTTC CTTCTACCAT TGATGAATAT GTTCATCGAA TTGGGCGTAC TGGTCGTTGT 
GGGAATACTG GCAGAGCAAT TTCCTTTTTT GATCTTGAAT CGGATAACCA TTTAGCACAG 
CCTCTAGTAA AAGTATTGAC AGATGCTCAA CAGGATGTTC CTGCATGGTT GGAAGAAATT 
GCCTTTAGTA CATACATTCC TGGCTTCAGT GGTAGTACAA GAGGAAACGT GTTTGCATCA 
GTTGATACCA GAAAGGGCAA GAGCACTTTG AACACAGCTG GGTTTTCTTC TTCACAAGCT 
CCCAATCCAG TAGATGATGA GTCATGGGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136021.1
CDS	100..2172
Translation	MGDEDWEAEINPHMSSYVPIFEKDRYSGENGDNFNRTPASSSEMDDGPSRRDHFMKSGFASGRNFGNRDAGECNKRDNTSTMGGFGVGKSFGNRGFSNSRFEDGDSSGFWRESSNDCEDNPTRNRGFSKRGDNDLDPDECMQRTGGLFGSRRPVLSGTGNGDTSQSRSGSGSERGGYKGLNEEVITGSGKNSWKSEAEGGESSDTQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHMMHDGITASRFKELQEPECIIVAPTRELVNQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLKQIKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAAEFLKSNYLFVAVGQVGGACRDVQQTVLQVGQFSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDTRKGKSTLNTAGFSSSQAPNPVDDESWD

Target ORF information:

RefSeq Version	NM_001142549.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 4 (DDX4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142549.1

ORF Insert Sequence:

ATGGGAGATG AAGATTGGGA AGCAGAAATC AACCCTCATA TGTCTTCCTA TGTTCCCATA 
TTTGAGAAGG ATAGGTATTC TGGAGAAAAT GGAGACAATT TTAACAGGAC TCCAGCTTCA 
TCATCAGAAA TGGATGATGG ACCTTCTCGA AGAGATCATT TCATGAAAAG TGGATTTGCC 
TCTGGGCGGA ATTTTGGAAA CAGAGATGCT GGTGAGTGTA ATAAGCGAGA TAATACATCC 
ACAATGGGTG GTTTTGGAGT TGGAAAGAGT TTTGGAAACA GAGGTTTTTC AAACAGCAGG 
TTTGAAGATG GTGATAGCTC TGGTTTCTGG AGAGAGTCTA GTAATGACTG CGAAGATAAT 
CCAACACGGA ACAGAGGGTT TTCCAAGAGA GGCGATAATG ACTTAGACCC AGACGAATGT 
ATGCAGCGCA CTGGTGGCCT TTTTGGTTCT AGAAGACCAG TATTAAGTGG CACAGGTAAT 
GGTGATACTT CTCAAAGCAG AAGTGGCAGT GGAAGTGAAC GAGGTGGTTA CAAAGGTTTA 
AATGAAGAAG TAATAACAGG CTCTGGAAAG AATTCTTGGA AGTCAGAAGC AGAAGGAGGA 
GAAAGTAGTG ATACTCAAGG ACCAAAAGTG ACCTACATAC CCCCTCCTCC ACCTGAGGAT 
GAGGACTCCA TCTTTGCACA TTATCAGACA GGCATAAACT TCGACAAATA CGACACTATT 
CTTGTGGAAG TGTCTGGACA TGATGCACCA CCAGCAATTC TGACTTTTGA AGAAGCTAAT 
CTCTGTCAGA CACTGAATAA CAACATTGCT AAAGCTGGTT ATACTAAGCT TACTCCTGTG 
CAAAAATACA GTATTCCTAT CATACTTGCA GGACGAGATT TGATGGCTTG CGCTCAAACA 
GGGTCTGGGA AGACTGCGGC TTTTCTCCTA CCAATTTTGG CTCATATGAT GCATGATGGA 
ATAACTGCCA GTCGTTTTAA AGAGTTGCAG GAACCAGAGT GTATTATTGT AGCACCAACT 
CGAGAATTGG TCAACCAGAT TTATTTGGAA GCCAGAAAAT TTTCTTTTGG GACTTGTGTA 
AGAGCTGTTG TTATATATGG GGGAACCCAG CTGGGACATT CAATTCGACA AATAGTACAA 
GGCTGTAATA TATTATGTGC TACTCCTGGA AGACTGATGG ATATCATAGG CAAAGAAAAG 
ATTGGTCTCA AACAGATCAA ATACTTAGTT TTGGATGAAG CTGATCGCAT GTTGGATATG 
GGTTTTGGTC CAGAAATGAA GAAGTTAATT TCTTGCCCAG GAATGCCATC AAAGGAACAG 
CGCCAAACCC TTATGTTCAG TGCAACTTTT CCAGAGGAAA TTCAAAGGTT GGCTGCAGAG 
TTTTTAAAGT CAAATTATCT GTTTGTTGCT GTTGGACAAG TGGGTGGAGC ATGTAGAGAT 
GTTCAGCAGA CCGTTCTCCA AGTTGGCCAG TTCTCAAAAA GAGAAAAGCT CGTTGAAATT 
CTGCGAAACA TAGGGGATGA AAGAACTATG GTCTTTGTTG AAACTAAGAA AAAAGCAGAT 
TTTATTGCAA CTTTTCTTTG TCAAGAAAAA ATATCAACTA CAAGTATTCA TGGTGATCGG 
GAACAGAGAG AGCGGGAGCA AGCTCTTGGA GATTTTCGCT TTGGAAAGTG CCCAGTTCTT 
GTTGCTACTT CAGTAGCTGC CAGAGGGCTG GATATTGAAA ATGTGCAACA TGTTATCAAT 
TTTGATCTTC CTTCTACCAT TGATGAATAT GTTCATCGAA TTGGGCGTAC TGGTCGTTGT 
GGGAATACTG GCAGAGCAAT TTCCTTTTTT GATCTTGAAT CGGATAACCA TTTAGCACAG 
CCTCTAGTAA AAGTATTGAC AGATGCTCAA CAGGATGTTC CTGCATGGTT GGAAGAAATT 
GCCTTTAGTA CATACATTCC TGGCTTCAGT GGTAGTACAA GAGGAAACGT GTTTGCATCA 
GTTGATACCA GAAAGGGCAA GAGCACTTTG AACACAGCTG GGTTTTCTTC TTCACAAGCT 
CCCAATCCAG TAGATGATGA GTCATGGGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX4 ( XM_011543495.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541797.1 Homo sapiens DEAD-box helicase 4 (DDX4), transcript variant X2, mRNA.

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CloneID	OHu68400
Clone ID Related Accession (Same CDS sequence)	XM_011543495.2
Accession Version	XM_011543495.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543495.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG AAGATTGGGA AGCAGAAATC AACCCTCATA TGTCTTCCTA TGTTCCCATA 
TTTGAGAAGG ATAGGTATTC TGGAGAAAAT GGAGACAATT TTAACAGGAC TCCAGCTTCA 
TCATCAGAAA TGGATGATGG ACCTTCTCGA AGAGATCATT TCATGAAAAG TGGATTTGCC 
TCTGGGCGGA ATTTTGGAAA CAGAGGTTTT TCAAACAGCA GGTTTGAAGA TGGTGATAGC 
TCTGGTTTCT GGAGAGAGTC TAGTAATGAC TGCGAAGATA ATCCAACACG GAACAGAGGG 
TTTTCCAAGA GAGGCGGCTA TCGAGATGGA AATAATTCAG AAGCTTCAGG GCCATACAGA 
AGAGGTGGAA GAGGTAGTTT CCGAGGTTGC CGTGGAGGAT TTGGTCTAGG AAGTCCAAAT 
AATGACTTAG ACCCAGACGA ATGTATGCAG CGCACTGGTG GCCTTTTTGG TTCTAGAAGA 
CCAGTATTAA GTGGCACAGG TAATGGTGAT ACTTCTCAAA GCAGAAGTGG CAGTGGAAGT 
GAACGAGGTG GTTACAAAGG TTTAAATGAA GAAGTAATAA CAGGCTCTGG AAAGAATTCT 
TGGAAGTCAG AAGCAGAAGG AGGAGAAAGT AGTGATACTC AAGGACCAAA AGTGACCTAC 
ATACCCCCTC CTCCACCTGA GGATGAGGAC TCCATCTTTG CACATTATCA GACAGGCATA 
AACTTCGACA AATACGACAC TATTCTTGTG GAAGTGTCTG GACATGATGC ACCACCAGCA 
ATTCTGACTT TTGAAGAAGC TAATCTCTGT CAGACACTGA ATAACAACAT TGCTAAAGCT 
GGTTATACTA AGCTTACTCC TGTGCAAAAA TACAGTATTC CTATCATACT TGCAGGACGA 
GATTTGATGG CTTGCGCTCA AACAGGGTCT GGGAAGACTG CGGCTTTTCT CCTACCAATT 
TTGGCTCATA TGATGCATGA TGGAATAACT GCCAGTCGTT TTAAAGAGTT GCAGGAACCA 
GAGTGTATTA TTGTAGCACC AACTCGAGAA TTGGTCAACC AGATTTATTT GGAAGCCAGA 
AAATTTTCTT TTGGGACTTG TGTAAGAGCT GTTGTTATAT ATGGGGGAAC CCAGCTGGGA 
CATTCAATTC GACAAATAGT ACAAGGCTGT AATATATTAT GTGCTACTCC TGGAAGACTG 
ATGGATATCA TAGGCAAAGA AAAGATTGGT CTCAAACAGA TCAAATACTT AGTTTTGGAT 
GAAGCTGATC GCATGTTGGA TATGGGTTTT GGTCCAGAAA TGAAGAAGTT AATTTCTTGC 
CCAGGAATGC CATCAAAGGA ACAGCGCCAA ACCCTTATGT TCAGTGCAAC TTTTCCAGAG 
GAAATTCAAA GGTTGGCTGC AGAGTTTTTA AAGTCAAATT ATCTGTTTGT TGCTGTTGGA 
CAAGTGGGTG GAGCATGTAG AGATGTTCAG CAGACCGTTC TCCAAGTTGG CCAGTTCTCA 
AAAAGAGAAA AGCTCGTTGA AATTCTGCGA AACATAGGGG ATGAAAGAAC TATGGTCTTT 
GTTGAAACTA AGAAAAAAGC AGATTTTATT GCAACTTTTC TTTGTCAAGA AAAAATATCA 
ACTACAAGTA TTCATGGTGA TCGGGAACAG AGAGAGCGGG AGCAAGCTCT TGGAGATTTT 
CGCTTTGGAA AGTGCCCAGT TCTTGTTGCT ACTTCAGTAG CTGCCAGAGG GCTGGATATT 
GAAAATGTGC AACATGTTAT CAATTTTGAT CTTCCTTCTA CCATTGATGA ATATGTTCAT 
CGAATTGGGC GTACTGGTCG TTGTGGGAAT ACTGGCAGAG CAATTTCCTT TTTTGATCTT 
GAATCGGATA ACCATTTAGC ACAGCCTCTA GTAAAAGTAT TGACAGATGC TCAACAGGAT 
GTTCCTGCAT GGTTGGAAGA AATTGCCTTT AGTACATACA TTCCTGGCTT CAGTGGTAGT 
ACAAGAGGAA ACGTGTTTGC ATCAGTTGAT ACCAGAAAGG GCAAGAGCAC TTTGAACACA 
GCTGGGTTTT CTTCTTCACA AGCTCCCAAT CCAGTAGATG ATGAGTCATG GGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541797.1
CDS	123..2219
Translation	MGDEDWEAEINPHMSSYVPIFEKDRYSGENGDNFNRTPASSSEMDDGPSRRDHFMKSGFASGRNFGNRGFSNSRFEDGDSSGFWRESSNDCEDNPTRNRGFSKRGGYRDGNNSEASGPYRRGGRGSFRGCRGGFGLGSPNNDLDPDECMQRTGGLFGSRRPVLSGTGNGDTSQSRSGSGSERGGYKGLNEEVITGSGKNSWKSEAEGGESSDTQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHMMHDGITASRFKELQEPECIIVAPTRELVNQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLKQIKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAAEFLKSNYLFVAVGQVGGACRDVQQTVLQVGQFSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDTRKGKSTLNTAGFSSSQAPNPVDDESWD

Target ORF information:

RefSeq Version	XM_011543495.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 4 (DDX4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543495.2

ORF Insert Sequence:

ATGGGAGATG AAGATTGGGA AGCAGAAATC AACCCTCATA TGTCTTCCTA TGTTCCCATA 
TTTGAGAAGG ATAGGTATTC TGGAGAAAAT GGAGACAATT TTAACAGGAC TCCAGCTTCA 
TCATCAGAAA TGGATGATGG ACCTTCTCGA AGAGATCATT TCATGAAAAG TGGATTTGCC 
TCTGGGCGGA ATTTTGGAAA CAGAGGTTTT TCAAACAGCA GGTTTGAAGA TGGTGATAGC 
TCTGGTTTCT GGAGAGAGTC TAGTAATGAC TGCGAAGATA ATCCAACACG GAACAGAGGG 
TTTTCCAAGA GAGGCGGCTA TCGAGATGGA AATAATTCAG AAGCTTCAGG GCCATACAGA 
AGAGGTGGAA GAGGTAGTTT CCGAGGTTGC CGTGGAGGAT TTGGTCTAGG AAGTCCAAAT 
AATGACTTAG ACCCAGACGA ATGTATGCAG CGCACTGGTG GCCTTTTTGG TTCTAGAAGA 
CCAGTATTAA GTGGCACAGG TAATGGTGAT ACTTCTCAAA GCAGAAGTGG CAGTGGAAGT 
GAACGAGGTG GTTACAAAGG TTTAAATGAA GAAGTAATAA CAGGCTCTGG AAAGAATTCT 
TGGAAGTCAG AAGCAGAAGG AGGAGAAAGT AGTGATACTC AAGGACCAAA AGTGACCTAC 
ATACCCCCTC CTCCACCTGA GGATGAGGAC TCCATCTTTG CACATTATCA GACAGGCATA 
AACTTCGACA AATACGACAC TATTCTTGTG GAAGTGTCTG GACATGATGC ACCACCAGCA 
ATTCTGACTT TTGAAGAAGC TAATCTCTGT CAGACACTGA ATAACAACAT TGCTAAAGCT 
GGTTATACTA AGCTTACTCC TGTGCAAAAA TACAGTATTC CTATCATACT TGCAGGACGA 
GATTTGATGG CTTGCGCTCA AACAGGGTCT GGGAAGACTG CGGCTTTTCT CCTACCAATT 
TTGGCTCATA TGATGCATGA TGGAATAACT GCCAGTCGTT TTAAAGAGTT GCAGGAACCA 
GAGTGTATTA TTGTAGCACC AACTCGAGAA TTGGTCAACC AGATTTATTT GGAAGCCAGA 
AAATTTTCTT TTGGGACTTG TGTAAGAGCT GTTGTTATAT ATGGGGGAAC CCAGCTGGGA 
CATTCAATTC GACAAATAGT ACAAGGCTGT AATATATTAT GTGCTACTCC TGGAAGACTG 
ATGGATATCA TAGGCAAAGA AAAGATTGGT CTCAAACAGA TCAAATACTT AGTTTTGGAT 
GAAGCTGATC GCATGTTGGA TATGGGTTTT GGTCCAGAAA TGAAGAAGTT AATTTCTTGC 
CCAGGAATGC CATCAAAGGA ACAGCGCCAA ACCCTTATGT TCAGTGCAAC TTTTCCAGAG 
GAAATTCAAA GGTTGGCTGC AGAGTTTTTA AAGTCAAATT ATCTGTTTGT TGCTGTTGGA 
CAAGTGGGTG GAGCATGTAG AGATGTTCAG CAGACCGTTC TCCAAGTTGG CCAGTTCTCA 
AAAAGAGAAA AGCTCGTTGA AATTCTGCGA AACATAGGGG ATGAAAGAAC TATGGTCTTT 
GTTGAAACTA AGAAAAAAGC AGATTTTATT GCAACTTTTC TTTGTCAAGA AAAAATATCA 
ACTACAAGTA TTCATGGTGA TCGGGAACAG AGAGAGCGGG AGCAAGCTCT TGGAGATTTT 
CGCTTTGGAA AGTGCCCAGT TCTTGTTGCT ACTTCAGTAG CTGCCAGAGG GCTGGATATT 
GAAAATGTGC AACATGTTAT CAATTTTGAT CTTCCTTCTA CCATTGATGA ATATGTTCAT 
CGAATTGGGC GTACTGGTCG TTGTGGGAAT ACTGGCAGAG CAATTTCCTT TTTTGATCTT 
GAATCGGATA ACCATTTAGC ACAGCCTCTA GTAAAAGTAT TGACAGATGC TCAACAGGAT 
GTTCCTGCAT GGTTGGAAGA AATTGCCTTT AGTACATACA TTCCTGGCTT CAGTGGTAGT 
ACAAGAGGAA ACGTGTTTGC ATCAGTTGAT ACCAGAAAGG GCAAGAGCAC TTTGAACACA 
GCTGGGTTTT CTTCTTCACA AGCTCCCAAT CCAGTAGATG ATGAGTCATG GGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX4 ( XM_011543497.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541799.1 Homo sapiens DEAD-box helicase 4 (DDX4), transcript variant X3, mRNA.

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CloneID	OHu68402
Clone ID Related Accession (Same CDS sequence)	XM_011543497.2
Accession Version	XM_011543497.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543497.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG AAGATTGGGA AGCAGAAATC AACCCTCATA TGTCTTCCTA TGTTCCCATA 
TTTGAGAAGG ATAGGTATTC TGGAGAAAAT GGAGACAATT TTAACAGGAC TCCAGCTTCA 
TCATCAGAAA TGGATGATGG ACCTTCTCGA AGAGATCATT TCATGAAAAG TGGATTTGCC 
TCTGGGCGGA ATTTTGGAAA CAGAGGTTTT TCAAACAGCA GGTTTGAAGA TGGTGATAGC 
TCTGGTTTCT GGAGAGGCTA TCGAGATGGA AATAATTCAG AAGCTTCAGG GCCATACAGA 
AGAGGTGGAA GAGGTAGTTT CCGAGGTTGC CGTGGAGGAT TTGGTCTAGG AAGTCCAAAT 
AATGACTTAG ACCCAGACGA ATGTATGCAG CGCACTGGTG GCCTTTTTGG TTCTAGAAGA 
CCAGTATTAA GTGGCACAGG TAATGGTGAT ACTTCTCAAA GCAGAAGTGG CAGTGGAAGT 
GAACGAGGTG GTTACAAAGG TTTAAATGAA GAAGTAATAA CAGGCTCTGG AAAGAATTCT 
TGGAAGTCAG AAGCAGAAGG AGGAGAAAGT AGTGATACTC AAGGACCAAA AGTGACCTAC 
ATACCCCCTC CTCCACCTGA GGATGAGGAC TCCATCTTTG CACATTATCA GACAGGCATA 
AACTTCGACA AATACGACAC TATTCTTGTG GAAGTGTCTG GACATGATGC ACCACCAGCA 
ATTCTGACTT TTGAAGAAGC TAATCTCTGT CAGACACTGA ATAACAACAT TGCTAAAGCT 
GGTTATACTA AGCTTACTCC TGTGCAAAAA TACAGTATTC CTATCATACT TGCAGGACGA 
GATTTGATGG CTTGCGCTCA AACAGGGTCT GGGAAGACTG CGGCTTTTCT CCTACCAATT 
TTGGCTCATA TGATGCATGA TGGAATAACT GCCAGTCGTT TTAAAGAGTT GCAGGAACCA 
GAGTGTATTA TTGTAGCACC AACTCGAGAA TTGGTCAACC AGATTTATTT GGAAGCCAGA 
AAATTTTCTT TTGGGACTTG TGTAAGAGCT GTTGTTATAT ATGGGGGAAC CCAGCTGGGA 
CATTCAATTC GACAAATAGT ACAAGGCTGT AATATATTAT GTGCTACTCC TGGAAGACTG 
ATGGATATCA TAGGCAAAGA AAAGATTGGT CTCAAACAGA TCAAATACTT AGTTTTGGAT 
GAAGCTGATC GCATGTTGGA TATGGGTTTT GGTCCAGAAA TGAAGAAGTT AATTTCTTGC 
CCAGGAATGC CATCAAAGGA ACAGCGCCAA ACCCTTATGT TCAGTGCAAC TTTTCCAGAG 
GAAATTCAAA GGTTGGCTGC AGAGTTTTTA AAGTCAAATT ATCTGTTTGT TGCTGTTGGA 
CAAGTGGGTG GAGCATGTAG AGATGTTCAG CAGACCGTTC TCCAAGTTGG CCAGTTCTCA 
AAAAGAGAAA AGCTCGTTGA AATTCTGCGA AACATAGGGG ATGAAAGAAC TATGGTCTTT 
GTTGAAACTA AGAAAAAAGC AGATTTTATT GCAACTTTTC TTTGTCAAGA AAAAATATCA 
ACTACAAGTA TTCATGGTGA TCGGGAACAG AGAGAGCGGG AGCAAGCTCT TGGAGATTTT 
CGCTTTGGAA AGTGCCCAGT TCTTGTTGCT ACTTCAGTAG CTGCCAGAGG GCTGGATATT 
GAAAATGTGC AACATGTTAT CAATTTTGAT CTTCCTTCTA CCATTGATGA ATATGTTCAT 
CGAATTGGGC GTACTGGTCG TTGTGGGAAT ACTGGCAGAG CAATTTCCTT TTTTGATCTT 
GAATCGGATA ACCATTTAGC ACAGCCTCTA GTAAAAGTAT TGACAGATGC TCAACAGGAT 
GTTCCTGCAT GGTTGGAAGA AATTGCCTTT AGTACATACA TTCCTGGCTT CAGTGGTAGT 
ACAAGAGGAA ACGTGTTTGC ATCAGTTGAT ACCAGAAAGG GCAAGAGCAC TTTGAACACA 
GCTGGGTTTT CTTCTTCACA AGCTCCCAAT CCAGTAGATG ATGAGTCATG GGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541799.1
CDS	123..2159
Translation	MGDEDWEAEINPHMSSYVPIFEKDRYSGENGDNFNRTPASSSEMDDGPSRRDHFMKSGFASGRNFGNRGFSNSRFEDGDSSGFWRGYRDGNNSEASGPYRRGGRGSFRGCRGGFGLGSPNNDLDPDECMQRTGGLFGSRRPVLSGTGNGDTSQSRSGSGSERGGYKGLNEEVITGSGKNSWKSEAEGGESSDTQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHMMHDGITASRFKELQEPECIIVAPTRELVNQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLKQIKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAAEFLKSNYLFVAVGQVGGACRDVQQTVLQVGQFSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDTRKGKSTLNTAGFSSSQAPNPVDDESWD

Target ORF information:

RefSeq Version	XM_011543497.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 4 (DDX4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543497.2

ORF Insert Sequence:

ATGGGAGATG AAGATTGGGA AGCAGAAATC AACCCTCATA TGTCTTCCTA TGTTCCCATA 
TTTGAGAAGG ATAGGTATTC TGGAGAAAAT GGAGACAATT TTAACAGGAC TCCAGCTTCA 
TCATCAGAAA TGGATGATGG ACCTTCTCGA AGAGATCATT TCATGAAAAG TGGATTTGCC 
TCTGGGCGGA ATTTTGGAAA CAGAGGTTTT TCAAACAGCA GGTTTGAAGA TGGTGATAGC 
TCTGGTTTCT GGAGAGGCTA TCGAGATGGA AATAATTCAG AAGCTTCAGG GCCATACAGA 
AGAGGTGGAA GAGGTAGTTT CCGAGGTTGC CGTGGAGGAT TTGGTCTAGG AAGTCCAAAT 
AATGACTTAG ACCCAGACGA ATGTATGCAG CGCACTGGTG GCCTTTTTGG TTCTAGAAGA 
CCAGTATTAA GTGGCACAGG TAATGGTGAT ACTTCTCAAA GCAGAAGTGG CAGTGGAAGT 
GAACGAGGTG GTTACAAAGG TTTAAATGAA GAAGTAATAA CAGGCTCTGG AAAGAATTCT 
TGGAAGTCAG AAGCAGAAGG AGGAGAAAGT AGTGATACTC AAGGACCAAA AGTGACCTAC 
ATACCCCCTC CTCCACCTGA GGATGAGGAC TCCATCTTTG CACATTATCA GACAGGCATA 
AACTTCGACA AATACGACAC TATTCTTGTG GAAGTGTCTG GACATGATGC ACCACCAGCA 
ATTCTGACTT TTGAAGAAGC TAATCTCTGT CAGACACTGA ATAACAACAT TGCTAAAGCT 
GGTTATACTA AGCTTACTCC TGTGCAAAAA TACAGTATTC CTATCATACT TGCAGGACGA 
GATTTGATGG CTTGCGCTCA AACAGGGTCT GGGAAGACTG CGGCTTTTCT CCTACCAATT 
TTGGCTCATA TGATGCATGA TGGAATAACT GCCAGTCGTT TTAAAGAGTT GCAGGAACCA 
GAGTGTATTA TTGTAGCACC AACTCGAGAA TTGGTCAACC AGATTTATTT GGAAGCCAGA 
AAATTTTCTT TTGGGACTTG TGTAAGAGCT GTTGTTATAT ATGGGGGAAC CCAGCTGGGA 
CATTCAATTC GACAAATAGT ACAAGGCTGT AATATATTAT GTGCTACTCC TGGAAGACTG 
ATGGATATCA TAGGCAAAGA AAAGATTGGT CTCAAACAGA TCAAATACTT AGTTTTGGAT 
GAAGCTGATC GCATGTTGGA TATGGGTTTT GGTCCAGAAA TGAAGAAGTT AATTTCTTGC 
CCAGGAATGC CATCAAAGGA ACAGCGCCAA ACCCTTATGT TCAGTGCAAC TTTTCCAGAG 
GAAATTCAAA GGTTGGCTGC AGAGTTTTTA AAGTCAAATT ATCTGTTTGT TGCTGTTGGA 
CAAGTGGGTG GAGCATGTAG AGATGTTCAG CAGACCGTTC TCCAAGTTGG CCAGTTCTCA 
AAAAGAGAAA AGCTCGTTGA AATTCTGCGA AACATAGGGG ATGAAAGAAC TATGGTCTTT 
GTTGAAACTA AGAAAAAAGC AGATTTTATT GCAACTTTTC TTTGTCAAGA AAAAATATCA 
ACTACAAGTA TTCATGGTGA TCGGGAACAG AGAGAGCGGG AGCAAGCTCT TGGAGATTTT 
CGCTTTGGAA AGTGCCCAGT TCTTGTTGCT ACTTCAGTAG CTGCCAGAGG GCTGGATATT 
GAAAATGTGC AACATGTTAT CAATTTTGAT CTTCCTTCTA CCATTGATGA ATATGTTCAT 
CGAATTGGGC GTACTGGTCG TTGTGGGAAT ACTGGCAGAG CAATTTCCTT TTTTGATCTT 
GAATCGGATA ACCATTTAGC ACAGCCTCTA GTAAAAGTAT TGACAGATGC TCAACAGGAT 
GTTCCTGCAT GGTTGGAAGA AATTGCCTTT AGTACATACA TTCCTGGCTT CAGTGGTAGT 
ACAAGAGGAA ACGTGTTTGC ATCAGTTGAT ACCAGAAAGG GCAAGAGCAC TTTGAACACA 
GCTGGGTTTT CTTCTTCACA AGCTCCCAAT CCAGTAGATG ATGAGTCATG GGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Does detection of DDX4 mRNA in cell-free seminal plasma represents a reliable noninvasive germ cell marker in patients with nonobstructive azoospermia?
Andrologia49(8)(2017 Oct)
Abdallah W,Hashad D,Abdelmaksoud R,Hashad MM

Germline factor DDX4 functions in blood-derived cancer cell phenotypes.
Cancer science108(8)1612-1619(2017 Aug)
Schudrowitz N,Takagi S,Wessel GM,Yajima M

Causes and Clinical Features of Infertile Men With Nonobstructive Azoospermia and Histopathologic Diagnosis of Hypospermatogenesis.
Urology10562-68(2017 Jul)
Cheng YS,Lu CW,Lin TY,Lin PY,Lin YM

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DDX4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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