RHOF cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RHOF
Entrez Gene ID	54509
Full Name	ras homolog family member F, filopodia associated
Synonyms	ARHF,RIF
General protein information	Preferred Names ras homolog family member F, filopodia associated Names rho-related GTP-binding protein RhoF ras homolog family member F (in filopodia) ras homolog gene family, member F (in filopodia) rho family GTPase Rif rho in filopodia
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.31

mRNA and Protein(s)

mRNA	Protein	Name
NM_019034.3	NP_061907.2	rho-related GTP-binding protein RhoF precursor
NM_019034.2	NP_061907.2	rho-related GTP-binding protein RhoF precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RHOF	XP_520654.2
Gallus gallus (chicken)	RHOF	NP_001265081.1
Canis lupus familiaris (dog)	RHOF	XP_853451.1
Rattus norvegicus (Norway rat)	Rhof	XP_001073389.2
Homo sapiens (human)	RHOF	NP_061907.2
Bos taurus (cattle)	RHOF	NP_001035692.1
Mus musculus (house mouse)	Rhof	NP_780301.1
Danio rerio (zebrafish)	rhof	NP_001018478.1
Xenopus tropicalis (tropical clawed frog)	rhof	NP_001016369.1

Gene Ontology
Bibliography

Related articles in PubMed

The Rif GTPase regulates cytoskeletal signaling from plexinA4 to promote neurite retraction.
Fan L, Yan H, Pellegrin S, Morigen, Mellor H
Neuroscience letters590178-83(2015 Mar)

The small Rho GTPase Rif and actin cytoskeletal remodelling.
Fan L, Mellor H
Biochemical Society transactions40(1)268-72(2012 Feb)

The interplay between the Escherichia coli Rho guanine nucleotide exchange factor effectors and the mammalian RhoGEF inhibitor EspH.
Wong AR, Clements A, Raymond B, Crepin VF, Frankel G
mBio3(1)(2012)

The small GTPase Rif is an alternative trigger for the formation of actin stress fibers in epithelial cells.
Fan L, Pellegrin S, Scott A, Mellor H
Journal of cell science123(Pt 8)1247-52(2010 Apr)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rif has two separate roles in FARP1 regulation-in controlling its association with plexinA4, and in releasing active RhoA from a plexinA4/FARP1 complex.
Title: The Rif GTPase regulates cytoskeletal signaling from plexinA4 to promote neurite retraction.

In the present review, our aim is to summarize the current information about the Rif effectors and how Rif remodels actin cytoskeleton in many aspects. [Review]
Title: The small Rho GTPase Rif and actin cytoskeletal remodelling.

The authors show that enteropathogenic Escherichia coli translocates EspH, which inactivates mammalian RhoGEFs and triggers cytotoxicity.
Title: The interplay between the Escherichia coli Rho guanine nucleotide exchange factor effectors and the mammalian RhoGEF inhibitor EspH.

Rif GTPase is an alternative trigger of stress-fiber formation in epithelial cells.
Title: The small GTPase Rif is an alternative trigger for the formation of actin stress fibers in epithelial cells.

deletion of genes RHOF in early-stage mycosis fungoides.
Title: Array-based comparative genomic hybridization in early-stage mycosis fungoides: recurrent deletion of tumor suppressor genes BCL7A, SMAC/DIABLO, and RHOF.

The following RHOF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RHOF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02410	NM_019034.3 Latest version!	Homo sapiens ras homolog family member F, filopodia associated (RHOF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
View Old Accession Versions >>
OHu02410	NM_019034.2	Homo sapiens ras homolog family member F, filopodia associated (RHOF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RHOF ( NM_019034.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_061907.2 Homo sapiens ras homolog family member F, filopodia associated (RHOF), mRNA.

Price & Availability↑

CloneID	OHu02410
Clone ID Related Accession (Same CDS sequence)	NM_019034.3 , NM_019034.2
Accession Version	NM_019034.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	rho-related GTP-binding protein RhoF precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG574506.1, BQ053707.1, BU527972.1, AK000254.1, BM830620.1, BQ422695.1 and CA426077.1. On Nov 22, 2018 this sequence version replaced NM_019034.2. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.583563.1, AK000254.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000267205.7/ ENSP00000267205.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGATGCCC CCGGGGCCCT GGCCCAGACC GCCGCCCCCG GTCCGGGCAG GAAGGAGCTG 
AAGATCGTGA TCGTGGGCGA CGGCGGCTGC GGCAAGACCT CGCTGCTCAT GGTGTACAGC 
CAGGGCTCCT TCCCCGAGCA CTACGCCCCA TCGGTGTTCG AGAAGTACAC GGCCAGCGTG 
ACCGTTGGCA GCAAGGAGGT GACCCTGAAC CTCTACGACA CGGCCGGGCA AGAAGACTAT 
GACCGGCTGC GGCCCCTGTC CTACCAGAAC ACCCACCTCG TGCTCATCTG CTATGACGTC 
ATGAATCCCA CCAGCTACGA CAACGTCCTC ATCAAGTGGT TCCCTGAGGT CACGCATTTC 
TGCCGCGGGA TCCCCATGGT GCTCATCGGC TGCAAGACAG ACCTGAGGAA GGACAAGGAG 
CAGCTGCGGA AGCTCCGGGC CGCCCAGCTG GAGCCCATCA CCTACATGCA GGGCCTGAGC 
GCCTGCGAAC AGATCCGAGC TGCTCTCTAC CTGGAATGTT CCGCCAAGTT TCGGGAGAAT 
GTGGAGGACG TCTTCCGGGA GGCCGCCAAG GTGGCTCTCA GCGCTCTGAA GAAGGCGCAA 
CGGCAGAAGA AGCGCCGGCT CTGCCTGCTG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061907.2
CDS	56..691
Translation	MDAPGALAQTAAPGPGRKELKIVIVGDGGCGKTSLLMVYSQGSFPEHYAPSVFEKYTASVTVGSKEVTLNLYDTAGQEDYDRLRPLSYQNTHLVLICYDVMNPTSYDNVLIKWFPEVTHFCRGIPMVLIGCKTDLRKDKEQLRKLRAAQLEPITYMQGLSACEQIRAALYLECSAKFRENVEDVFREAAKVALSALKKAQRQKKRRLCLLL

Target ORF information:

RefSeq Version	NM_019034.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ras homolog family member F, filopodia associated (RHOF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019034.3

ORF Insert Sequence:

ATGGATGCCC CCGGGGCCCT GGCCCAGACC GCCGCCCCCG GTCCGGGCAG GAAGGAGCTG 
AAGATCGTGA TCGTGGGCGA CGGCGGCTGC GGCAAGACCT CGCTGCTCAT GGTGTACAGC 
CAGGGCTCCT TCCCCGAGCA CTACGCCCCA TCGGTGTTCG AGAAGTACAC GGCCAGCGTG 
ACCGTTGGCA GCAAGGAGGT GACCCTGAAC CTCTACGACA CGGCCGGGCA AGAAGACTAT 
GACCGGCTGC GGCCCCTGTC CTACCAGAAC ACCCACCTCG TGCTCATCTG CTATGACGTC 
ATGAATCCCA CCAGCTACGA CAACGTCCTC ATCAAGTGGT TCCCTGAGGT CACGCATTTC 
TGCCGCGGGA TCCCCATGGT GCTCATCGGC TGCAAGACAG ACCTGAGGAA GGACAAGGAG 
CAGCTGCGGA AGCTCCGGGC CGCCCAGCTG GAGCCCATCA CCTACATGCA GGGCCTGAGC 
GCCTGCGAAC AGATCCGAGC TGCTCTCTAC CTGGAATGTT CCGCCAAGTT TCGGGAGAAT 
GTGGAGGACG TCTTCCGGGA GGCCGCCAAG GTGGCTCTCA GCGCTCTGAA GAAGGCGCAA 
CGGCAGAAGA AGCGCCGGCT CTGCCTGCTG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHOF ( NM_019034.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_061907.2 Homo sapiens ras homolog family member F, filopodia associated (RHOF), mRNA.

Price & Availability↑

CloneID	OHu02410
Clone ID Related Accession (Same CDS sequence)	NM_019034.3 , NM_019034.2
Accession Version	NM_019034.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	rho-related GTP-binding protein RhoF precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG574506.1, BQ053707.1, BU527972.1, AK000254.1, BM830620.1, BQ422695.1 and CA426077.1. On Oct 29, 2003 this sequence version replaced NM_019034.1. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.326927.1, AK000254.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGATGCCC CCGGGGCCCT GGCCCAGACC GCCGCCCCCG GTCCGGGCAG GAAGGAGCTG 
AAGATCGTGA TCGTGGGCGA CGGCGGCTGC GGCAAGACCT CGCTGCTCAT GGTGTACAGC 
CAGGGCTCCT TCCCCGAGCA CTACGCCCCA TCGGTGTTCG AGAAGTACAC GGCCAGCGTG 
ACCGTTGGCA GCAAGGAGGT GACCCTGAAC CTCTACGACA CGGCCGGGCA AGAAGACTAT 
GACCGGCTGC GGCCCCTGTC CTACCAGAAC ACCCACCTCG TGCTCATCTG CTATGACGTC 
ATGAATCCCA CCAGCTACGA CAACGTCCTC ATCAAGTGGT TCCCTGAGGT CACGCATTTC 
TGCCGCGGGA TCCCCATGGT GCTCATCGGC TGCAAGACAG ACCTGAGGAA GGACAAGGAG 
CAGCTGCGGA AGCTCCGGGC CGCCCAGCTG GAGCCCATCA CCTACATGCA GGGCCTGAGC 
GCCTGCGAAC AGATCCGAGC TGCTCTCTAC CTGGAATGTT CCGCCAAGTT TCGGGAGAAT 
GTGGAGGACG TCTTCCGGGA GGCCGCCAAG GTGGCTCTCA GCGCTCTGAA GAAGGCGCAA 
CGGCAGAAGA AGCGCCGGCT CTGCCTGCTG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061907.2
CDS	56..691
Translation	MDAPGALAQTAAPGPGRKELKIVIVGDGGCGKTSLLMVYSQGSFPEHYAPSVFEKYTASVTVGSKEVTLNLYDTAGQEDYDRLRPLSYQNTHLVLICYDVMNPTSYDNVLIKWFPEVTHFCRGIPMVLIGCKTDLRKDKEQLRKLRAAQLEPITYMQGLSACEQIRAALYLECSAKFRENVEDVFREAAKVALSALKKAQRQKKRRLCLLL

Target ORF information:

RefSeq Version	NM_019034.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ras homolog family member F, filopodia associated (RHOF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019034.2

ORF Insert Sequence:

ATGGATGCCC CCGGGGCCCT GGCCCAGACC GCCGCCCCCG GTCCGGGCAG GAAGGAGCTG 
AAGATCGTGA TCGTGGGCGA CGGCGGCTGC GGCAAGACCT CGCTGCTCAT GGTGTACAGC 
CAGGGCTCCT TCCCCGAGCA CTACGCCCCA TCGGTGTTCG AGAAGTACAC GGCCAGCGTG 
ACCGTTGGCA GCAAGGAGGT GACCCTGAAC CTCTACGACA CGGCCGGGCA AGAAGACTAT 
GACCGGCTGC GGCCCCTGTC CTACCAGAAC ACCCACCTCG TGCTCATCTG CTATGACGTC 
ATGAATCCCA CCAGCTACGA CAACGTCCTC ATCAAGTGGT TCCCTGAGGT CACGCATTTC 
TGCCGCGGGA TCCCCATGGT GCTCATCGGC TGCAAGACAG ACCTGAGGAA GGACAAGGAG 
CAGCTGCGGA AGCTCCGGGC CGCCCAGCTG GAGCCCATCA CCTACATGCA GGGCCTGAGC 
GCCTGCGAAC AGATCCGAGC TGCTCTCTAC CTGGAATGTT CCGCCAAGTT TCGGGAGAAT 
GTGGAGGACG TCTTCCGGGA GGCCGCCAAG GTGGCTCTCA GCGCTCTGAA GAAGGCGCAA 
CGGCAGAAGA AGCGCCGGCT CTGCCTGCTG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Rif GTPase regulates cytoskeletal signaling from plexinA4 to promote neurite retraction.
Neuroscience letters590178-83(2015 Mar)
Fan L,Yan H,Pellegrin S,Morigen,Mellor H

The small Rho GTPase Rif and actin cytoskeletal remodelling.
Biochemical Society transactions40(1)268-72(2012 Feb)
Fan L,Mellor H

The interplay between the Escherichia coli Rho guanine nucleotide exchange factor effectors and the mammalian RhoGEF inhibitor EspH.
mBio3(1)(2012)
Wong AR,Clements A,Raymond B,Crepin VF,Frankel G

The small GTPase Rif is an alternative trigger for the formation of actin stress fibers in epithelial cells.
Journal of cell science123(Pt 8)1247-52(2010 Apr)
Fan L,Pellegrin S,Scott A,Mellor H

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

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RHOF cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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