POU1F1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	POU1F1
Entrez Gene ID	5449
Full Name	POU class 1 homeobox 1
Synonyms	CPHD1,GHF-1,PIT1,POU1F1a,Pit-1
General protein information	Preferred Names POU class 1 homeobox 1 Names pituitary-specific positive transcription factor 1 POU domain, class 1, transcription factor 1 growth hormone factor 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p11.2
Summary	This gene encodes a member of the POU family of transcription factors that regulate mammalian development. The protein regulates expression of several genes involved in pituitary development and hormone expression. Mutations in this genes result in combined pituitary hormone deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	173110
Disorder Html:	Pituitary hormone deficiency, combined, 1, 613038 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000306.3	NP_000297.1	pituitary-specific positive transcription factor 1 isoform alpha
NM_001122757.3	NP_001116229.1	pituitary-specific positive transcription factor 1 isoform beta
NM_001122757.2	NP_001116229.1	pituitary-specific positive transcription factor 1 isoform beta
NM_000306.4	NP_000297.1	pituitary-specific positive transcription factor 1 isoform alpha

Pathways from BioSystems

Homologies

Homo sapiens (human)	POU1F1	NP_001116229.1
Canis lupus familiaris (dog)	POU1F1	NP_001006950.1
Xenopus tropicalis (tropical clawed frog)	pou1f1	XP_002931806.1
Pan troglodytes (chimpanzee)	POU1F1	XP_003318250.1
	POU1F1	NP_001036325.1
Danio rerio (zebrafish)	pou1f1	NP_998016.1
Mus musculus (house mouse)	Pou1f1	NP_032875.1
Gallus gallus (chicken)	POU1F1	NP_989650.1
Bos taurus (cattle)	POU1F1	NP_777004.1
Rattus norvegicus (Norway rat)	Pou1f1	NP_037140.2

Gene Ontology
Bibliography

Related articles in PubMed

Biochemical and structural characterization of a novel cooperative binding mode by Pit-1 with CATT repeats in the macrophage migration inhibitory factor promoter.
Agarwal S, Cho TY
Nucleic acids research(2017 Nov)

Combined pituitary hormone deficiency due to gross deletions in the POU1F1 (PIT-1) and PROP1 genes.
Bertko E, Klammt J, Dusatkova P, Bahceci M, Gonc N, Ten Have L, Kandemir N, Mansmann G, Obermannova B, Oostdijk W, Pf?ffle H, Rockstroh-Lippold D, Schlicke M, Tuzcu AK, Pf?ffle R
Journal of human genetics62(8)755-762(2017 Aug)

A novel heterozygous intronic mutation in POU1F1 is associated with combined pituitary hormone deficiency.
Takagi M, Kamasaki H, Yagi H, Fukuzawa R, Narumi S, Hasegawa T
Endocrine journal64(2)229-234(2017 Feb)

Over-Expression of POU Class 1 Homeobox 1 Transcription Factor (Pit-1) Predicts Poor Prognosis for Breast Cancer Patients.
Gao Z, Xue K, Zhang L, Wei M
Medical science monitor : international medical journal of experimental and clinical research224121-4125(2016 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This result strongly suggests the possibility, for the first time, that isolated mutations in the short isoform of POU1F1 could be sufficient for induction of POU1F1-related combined pituitary hormone deficiency.
Title: A novel heterozygous intronic mutation in POU1F1 is associated with combined pituitary hormone deficiency.

Data suggest that the first screening step diagnosis for pituitary adenoma was determined based upon immunohistochemical (IHC) scores for Pit-1, SF-1, and ACTH.
Title: Steroidogenic Factor 1, Pit-1, and Adrenocorticotropic Hormone: A Rational Starting Place for the Immunohistochemical Characterization of Pituitary Adenoma.

The present study reports on screening of POU1F1, PROP1, and HESX1 in combined pituitary hormone deficiency patients and the novel variations identified.
Title: Identification of Novel PROP1 and POU1F1 Mutations in Patients with Combined Pituitary Hormone Deficiency.

High expression level of Pit-1 was significantly associated with poor overall survival of the patients (P=0.001) and Cox regression analysis indicated that Pit-1 might be a prognostic factor for breast cancer prognosis (HR=1.955, 95% CI=1.295-3.035, P=0.003).
Title: Over-Expression of POU Class 1 Homeobox 1 Transcription Factor (Pit-1) Predicts Poor Prognosis for Breast Cancer Patients.

Data identify silent subtype 3 adenomas as aggressive monomorphous plurihormonal adenomas of Pit-1 lineage that may be associated with hyperthyroidism, acromegaly or galactorrhea and amenorrhea.
Title: Silent subtype 3 pituitary adenomas are not always silent and represent poorly differentiated monomorphous plurihormonal Pit-1 lineage adenomas.

The following POU1F1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POU1F1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23455	NM_001122757.3 Latest version!	Homo sapiens POU class 1 homeobox 1 (POU1F1), transcript variant beta, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu23957	NM_000306.4 Latest version!	Homo sapiens POU class 1 homeobox 1 (POU1F1), transcript variant alpha, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
View Old Accession Versions >>
OHu23455	NM_001122757.2	Homo sapiens POU class 1 homeobox 1 (POU1F1), transcript variant beta, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu23957	NM_000306.3	Homo sapiens POU class 1 homeobox 1 (POU1F1), transcript variant alpha, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POU1F1 ( NM_001122757.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001116229.1 Homo sapiens POU class 1 homeobox 1 (POU1F1), transcript variant beta, mRNA.

Price & Availability↑

CloneID	OHu23455
Clone ID Related Accession (Same CDS sequence)	NM_001122757.3 , NM_001122757.2
Accession Version	NM_001122757.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	pituitary-specific positive transcription factor 1 isoform beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC130885.3 and KF457648.1. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_001122757.2. Transcript Variant: This variant (beta), also known as GHF-2 or PIT-2, represents the longer transcript and encodes the longer isoform (beta). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2158188, SAMEA2162895 [ECO:0000350] ##Evidence-Data-END##

ATGAGTTGCC AAGCTTTTAC TTCGGCTGAT ACCTTTATAC CTCTGAATTC TGACGCCTCT 
GCAACTCTGC CTCTGATAAT GCATCACAGT GCTGCCGAGT GTCTACCAGT CTCCAACCAT 
GCCACCAATG TGATGTCTAC AGTCCCATCT ATTTTGTCTT TGATCCAAAC TCCTAAATGT 
TTGTGCACAC ATTTCTCGGT GACAACGTTG GGAAACACAG CAACAGGACT TCATTATTCT 
GTTCCTTCCT GTCATTATGG AAACCAGCCA TCAACCTATG GAGTGATGGC AGGTAGTTTA 
ACCCCTTGTC TTTATAAATT TCCTGACCAC ACCTTGAGTC ATGGATTTCC TCCTATACAC 
CAGCCTCTTC TGGCAGAGGA CCCCACAGCT GCTGATTTCA AGCAGGAACT CAGGCGGAAA 
AGTAAATTGG TGGAAGAGCC AATAGACATG GATTCTCCAG AAATCAGAGA ACTTGAAAAG 
TTTGCCAATG AATTTAAAGT GAGACGAATT AAATTAGGAT ACACCCAGAC AAATGTTGGG 
GAGGCCCTGG CAGCTGTGCA TGGCTCTGAA TTCAGTCAAA CAACAATCTG CCGATTTGAA 
AATCTGCAGC TCAGCTTTAA AAATGCATGC AAACTGAAAG CAATATTATC CAAATGGCTG 
GAGGAAGCTG AGCAAGTAGG AGCTTTGTAC AATGAAAAAG TGGGAGCAAA TGAAAGGAAA 
AGAAAACGAA GAACAACTAT AAGCATTGCT GCTAAAGATG CTCTGGAGAG ACACTTTGGA 
GAACAGAATA AACCTTCTTC TCAAGAGATC ATGAGGATGG CTGAAGAACT GAATCTGGAG 
AAAGAAGTAG TAAGAGTTTG GTTTTGCAAC CGGAGGCAGA GAGAAAAACG GGTGAAAACA 
AGTCTGAATC AGAGTTTATT TTCTATTTCT AAGGAACATC TTGAGTGCAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116229.1
CDS	123..1076
Translation	MSCQAFTSADTFIPLNSDASATLPLIMHHSAAECLPVSNHATNVMSTVPSILSLIQTPKCLCTHFSVTTLGNTATGLHYSVPSCHYGNQPSTYGVMAGSLTPCLYKFPDHTLSHGFPPIHQPLLAEDPTAADFKQELRRKSKLVEEPIDMDSPEIRELEKFANEFKVRRIKLGYTQTNVGEALAAVHGSEFSQTTICRFENLQLSFKNACKLKAILSKWLEEAEQVGALYNEKVGANERKRKRRTTISIAAKDALERHFGEQNKPSSQEIMRMAEELNLEKEVVRVWFCNRRQREKRVKTSLNQSLFSISKEHLECR

Target ORF information:

RefSeq Version	NM_001122757.3
Organism	Homo sapiens(human)
Definition	Homo sapiens POU class 1 homeobox 1 (POU1F1), transcript variant beta, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001122757.3

ORF Insert Sequence:

ATGAGTTGCC AAGCTTTTAC TTCGGCTGAT ACCTTTATAC CTCTGAATTC TGACGCCTCT 
GCAACTCTGC CTCTGATAAT GCATCACAGT GCTGCCGAGT GTCTACCAGT CTCCAACCAT 
GCCACCAATG TGATGTCTAC AGTCCCATCT ATTTTGTCTT TGATCCAAAC TCCTAAATGT 
TTGTGCACAC ATTTCTCGGT GACAACGTTG GGAAACACAG CAACAGGACT TCATTATTCT 
GTTCCTTCCT GTCATTATGG AAACCAGCCA TCAACCTATG GAGTGATGGC AGGTAGTTTA 
ACCCCTTGTC TTTATAAATT TCCTGACCAC ACCTTGAGTC ATGGATTTCC TCCTATACAC 
CAGCCTCTTC TGGCAGAGGA CCCCACAGCT GCTGATTTCA AGCAGGAACT CAGGCGGAAA 
AGTAAATTGG TGGAAGAGCC AATAGACATG GATTCTCCAG AAATCAGAGA ACTTGAAAAG 
TTTGCCAATG AATTTAAAGT GAGACGAATT AAATTAGGAT ACACCCAGAC AAATGTTGGG 
GAGGCCCTGG CAGCTGTGCA TGGCTCTGAA TTCAGTCAAA CAACAATCTG CCGATTTGAA 
AATCTGCAGC TCAGCTTTAA AAATGCATGC AAACTGAAAG CAATATTATC CAAATGGCTG 
GAGGAAGCTG AGCAAGTAGG AGCTTTGTAC AATGAAAAAG TGGGAGCAAA TGAAAGGAAA 
AGAAAACGAA GAACAACTAT AAGCATTGCT GCTAAAGATG CTCTGGAGAG ACACTTTGGA 
GAACAGAATA AACCTTCTTC TCAAGAGATC ATGAGGATGG CTGAAGAACT GAATCTGGAG 
AAAGAAGTAG TAAGAGTTTG GTTTTGCAAC CGGAGGCAGA GAGAAAAACG GGTGAAAACA 
AGTCTGAATC AGAGTTTATT TTCTATTTCT AAGGAACATC TTGAGTGCAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POU1F1 ( NM_001122757.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001116229.1 Homo sapiens POU class 1 homeobox 1 (POU1F1), transcript variant beta, mRNA.

Price & Availability↑

CloneID	OHu23455
Clone ID Related Accession (Same CDS sequence)	NM_001122757.3 , NM_001122757.2
Accession Version	NM_001122757.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	pituitary-specific positive transcription factor 1 isoform beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC130885.3 and KF457648.1. This sequence is a reference standard in the RefSeqGene project. On Jan 17, 2014 this sequence version replaced NM_001122757.1. Transcript Variant: This variant (beta), also known as GHF-2 or PIT-2, represents the longer transcript and encodes the longer isoform (beta). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2158188, SAMEA2162895 [ECO:0000350] ##Evidence-Data-END##

ATGAGTTGCC AAGCTTTTAC TTCGGCTGAT ACCTTTATAC CTCTGAATTC TGACGCCTCT 
GCAACTCTGC CTCTGATAAT GCATCACAGT GCTGCCGAGT GTCTACCAGT CTCCAACCAT 
GCCACCAATG TGATGTCTAC AGTCCCATCT ATTTTGTCTT TGATCCAAAC TCCTAAATGT 
TTGTGCACAC ATTTCTCGGT GACAACGTTG GGAAACACAG CAACAGGACT TCATTATTCT 
GTTCCTTCCT GTCATTATGG AAACCAGCCA TCAACCTATG GAGTGATGGC AGGTAGTTTA 
ACCCCTTGTC TTTATAAATT TCCTGACCAC ACCTTGAGTC ATGGATTTCC TCCTATACAC 
CAGCCTCTTC TGGCAGAGGA CCCCACAGCT GCTGATTTCA AGCAGGAACT CAGGCGGAAA 
AGTAAATTGG TGGAAGAGCC AATAGACATG GATTCTCCAG AAATCAGAGA ACTTGAAAAG 
TTTGCCAATG AATTTAAAGT GAGACGAATT AAATTAGGAT ACACCCAGAC AAATGTTGGG 
GAGGCCCTGG CAGCTGTGCA TGGCTCTGAA TTCAGTCAAA CAACAATCTG CCGATTTGAA 
AATCTGCAGC TCAGCTTTAA AAATGCATGC AAACTGAAAG CAATATTATC CAAATGGCTG 
GAGGAAGCTG AGCAAGTAGG AGCTTTGTAC AATGAAAAAG TGGGAGCAAA TGAAAGGAAA 
AGAAAACGAA GAACAACTAT AAGCATTGCT GCTAAAGATG CTCTGGAGAG ACACTTTGGA 
GAACAGAATA AACCTTCTTC TCAAGAGATC ATGAGGATGG CTGAAGAACT GAATCTGGAG 
AAAGAAGTAG TAAGAGTTTG GTTTTGCAAC CGGAGGCAGA GAGAAAAACG GGTGAAAACA 
AGTCTGAATC AGAGTTTATT TTCTATTTCT AAGGAACATC TTGAGTGCAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116229.1
CDS	126..1079
Translation	MSCQAFTSADTFIPLNSDASATLPLIMHHSAAECLPVSNHATNVMSTVPSILSLIQTPKCLCTHFSVTTLGNTATGLHYSVPSCHYGNQPSTYGVMAGSLTPCLYKFPDHTLSHGFPPIHQPLLAEDPTAADFKQELRRKSKLVEEPIDMDSPEIRELEKFANEFKVRRIKLGYTQTNVGEALAAVHGSEFSQTTICRFENLQLSFKNACKLKAILSKWLEEAEQVGALYNEKVGANERKRKRRTTISIAAKDALERHFGEQNKPSSQEIMRMAEELNLEKEVVRVWFCNRRQREKRVKTSLNQSLFSISKEHLECR

Target ORF information:

RefSeq Version	NM_001122757.2
Organism	Homo sapiens(human)
Definition	Homo sapiens POU class 1 homeobox 1 (POU1F1), transcript variant beta, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001122757.2

ORF Insert Sequence:

ATGAGTTGCC AAGCTTTTAC TTCGGCTGAT ACCTTTATAC CTCTGAATTC TGACGCCTCT 
GCAACTCTGC CTCTGATAAT GCATCACAGT GCTGCCGAGT GTCTACCAGT CTCCAACCAT 
GCCACCAATG TGATGTCTAC AGTCCCATCT ATTTTGTCTT TGATCCAAAC TCCTAAATGT 
TTGTGCACAC ATTTCTCGGT GACAACGTTG GGAAACACAG CAACAGGACT TCATTATTCT 
GTTCCTTCCT GTCATTATGG AAACCAGCCA TCAACCTATG GAGTGATGGC AGGTAGTTTA 
ACCCCTTGTC TTTATAAATT TCCTGACCAC ACCTTGAGTC ATGGATTTCC TCCTATACAC 
CAGCCTCTTC TGGCAGAGGA CCCCACAGCT GCTGATTTCA AGCAGGAACT CAGGCGGAAA 
AGTAAATTGG TGGAAGAGCC AATAGACATG GATTCTCCAG AAATCAGAGA ACTTGAAAAG 
TTTGCCAATG AATTTAAAGT GAGACGAATT AAATTAGGAT ACACCCAGAC AAATGTTGGG 
GAGGCCCTGG CAGCTGTGCA TGGCTCTGAA TTCAGTCAAA CAACAATCTG CCGATTTGAA 
AATCTGCAGC TCAGCTTTAA AAATGCATGC AAACTGAAAG CAATATTATC CAAATGGCTG 
GAGGAAGCTG AGCAAGTAGG AGCTTTGTAC AATGAAAAAG TGGGAGCAAA TGAAAGGAAA 
AGAAAACGAA GAACAACTAT AAGCATTGCT GCTAAAGATG CTCTGGAGAG ACACTTTGGA 
GAACAGAATA AACCTTCTTC TCAAGAGATC ATGAGGATGG CTGAAGAACT GAATCTGGAG 
AAAGAAGTAG TAAGAGTTTG GTTTTGCAAC CGGAGGCAGA GAGAAAAACG GGTGAAAACA 
AGTCTGAATC AGAGTTTATT TTCTATTTCT AAGGAACATC TTGAGTGCAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POU1F1 ( NM_000306.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000297.1 Homo sapiens POU class 1 homeobox 1 (POU1F1), transcript variant alpha, mRNA.

Price & Availability↑

CloneID	OHu23957
Clone ID Related Accession (Same CDS sequence)	NM_000306.3 , NM_000306.4
Accession Version	NM_000306.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	pituitary-specific positive transcription factor 1 isoform alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D10216.1 and KF457648.1. On Jan 17, 2014 this sequence version replaced NM_000306.2. Transcript Variant: This variant (alpha) uses an alternate in-frame splice site in the 5' coding region, compared to variant beta, resulting in a shorter protein (isoform alpha). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D10216.1, X62429.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2158188, SAMEA2162895 [ECO:0000350] ##Evidence-Data-END##

ATGAGTTGCC AAGCTTTTAC TTCGGCTGAT ACCTTTATAC CTCTGAATTC TGACGCCTCT 
GCAACTCTGC CTCTGATAAT GCATCACAGT GCTGCCGAGT GTCTACCAGT CTCCAACCAT 
GCCACCAATG TGATGTCTAC AGCAACAGGA CTTCATTATT CTGTTCCTTC CTGTCATTAT 
GGAAACCAGC CATCAACCTA TGGAGTGATG GCAGGTAGTT TAACCCCTTG TCTTTATAAA 
TTTCCTGACC ACACCTTGAG TCATGGATTT CCTCCTATAC ACCAGCCTCT TCTGGCAGAG 
GACCCCACAG CTGCTGATTT CAAGCAGGAA CTCAGGCGGA AAAGTAAATT GGTGGAAGAG 
CCAATAGACA TGGATTCTCC AGAAATCAGA GAACTTGAAA AGTTTGCCAA TGAATTTAAA 
GTGAGACGAA TTAAATTAGG ATACACCCAG ACAAATGTTG GGGAGGCCCT GGCAGCTGTG 
CATGGCTCTG AATTCAGTCA AACAACAATC TGCCGATTTG AAAATCTGCA GCTCAGCTTT 
AAAAATGCAT GCAAACTGAA AGCAATATTA TCCAAATGGC TGGAGGAAGC TGAGCAAGTA 
GGAGCTTTGT ACAATGAAAA AGTGGGAGCA AATGAAAGGA AAAGAAAACG AAGAACAACT 
ATAAGCATTG CTGCTAAAGA TGCTCTGGAG AGACACTTTG GAGAACAGAA TAAACCTTCT 
TCTCAAGAGA TCATGAGGAT GGCTGAAGAA CTGAATCTGG AGAAAGAAGT AGTAAGAGTT 
TGGTTTTGCA ACCGGAGGCA GAGAGAAAAA CGGGTGAAAA CAAGTCTGAA TCAGAGTTTA 
TTTTCTATTT CTAAGGAACA TCTTGAGTGC AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000297.1
CDS	126..1001
Translation	MSCQAFTSADTFIPLNSDASATLPLIMHHSAAECLPVSNHATNVMSTATGLHYSVPSCHYGNQPSTYGVMAGSLTPCLYKFPDHTLSHGFPPIHQPLLAEDPTAADFKQELRRKSKLVEEPIDMDSPEIRELEKFANEFKVRRIKLGYTQTNVGEALAAVHGSEFSQTTICRFENLQLSFKNACKLKAILSKWLEEAEQVGALYNEKVGANERKRKRRTTISIAAKDALERHFGEQNKPSSQEIMRMAEELNLEKEVVRVWFCNRRQREKRVKTSLNQSLFSISKEHLECR

Target ORF information:

RefSeq Version	NM_000306.3
Organism	Homo sapiens(human)
Definition	Homo sapiens POU class 1 homeobox 1 (POU1F1), transcript variant alpha, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000306.3

ORF Insert Sequence:

ATGAGTTGCC AAGCTTTTAC TTCGGCTGAT ACCTTTATAC CTCTGAATTC TGACGCCTCT 
GCAACTCTGC CTCTGATAAT GCATCACAGT GCTGCCGAGT GTCTACCAGT CTCCAACCAT 
GCCACCAATG TGATGTCTAC AGCAACAGGA CTTCATTATT CTGTTCCTTC CTGTCATTAT 
GGAAACCAGC CATCAACCTA TGGAGTGATG GCAGGTAGTT TAACCCCTTG TCTTTATAAA 
TTTCCTGACC ACACCTTGAG TCATGGATTT CCTCCTATAC ACCAGCCTCT TCTGGCAGAG 
GACCCCACAG CTGCTGATTT CAAGCAGGAA CTCAGGCGGA AAAGTAAATT GGTGGAAGAG 
CCAATAGACA TGGATTCTCC AGAAATCAGA GAACTTGAAA AGTTTGCCAA TGAATTTAAA 
GTGAGACGAA TTAAATTAGG ATACACCCAG ACAAATGTTG GGGAGGCCCT GGCAGCTGTG 
CATGGCTCTG AATTCAGTCA AACAACAATC TGCCGATTTG AAAATCTGCA GCTCAGCTTT 
AAAAATGCAT GCAAACTGAA AGCAATATTA TCCAAATGGC TGGAGGAAGC TGAGCAAGTA 
GGAGCTTTGT ACAATGAAAA AGTGGGAGCA AATGAAAGGA AAAGAAAACG AAGAACAACT 
ATAAGCATTG CTGCTAAAGA TGCTCTGGAG AGACACTTTG GAGAACAGAA TAAACCTTCT 
TCTCAAGAGA TCATGAGGAT GGCTGAAGAA CTGAATCTGG AGAAAGAAGT AGTAAGAGTT 
TGGTTTTGCA ACCGGAGGCA GAGAGAAAAA CGGGTGAAAA CAAGTCTGAA TCAGAGTTTA 
TTTTCTATTT CTAAGGAACA TCTTGAGTGC AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POU1F1 ( NM_000306.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000297.1 Homo sapiens POU class 1 homeobox 1 (POU1F1), transcript variant alpha, mRNA.

Price & Availability↑

CloneID	OHu23957
Clone ID Related Accession (Same CDS sequence)	NM_000306.3 , NM_000306.4
Accession Version	NM_000306.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	pituitary-specific positive transcription factor 1 isoform alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D10216.1, KF457648.1 and AC130885.3. On May 17, 2019 this sequence version replaced NM_000306.3. Transcript Variant: This variant (alpha) uses an alternate in-frame splice site in the 5' coding region, compared to variant beta, resulting in a shorter protein (isoform alpha). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D10216.1, X62429.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2158188, SAMEA2162895 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000350375.7/ ENSP00000263781.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END##

ATGAGTTGCC AAGCTTTTAC TTCGGCTGAT ACCTTTATAC CTCTGAATTC TGACGCCTCT 
GCAACTCTGC CTCTGATAAT GCATCACAGT GCTGCCGAGT GTCTACCAGT CTCCAACCAT 
GCCACCAATG TGATGTCTAC AGCAACAGGA CTTCATTATT CTGTTCCTTC CTGTCATTAT 
GGAAACCAGC CATCAACCTA TGGAGTGATG GCAGGTAGTT TAACCCCTTG TCTTTATAAA 
TTTCCTGACC ACACCTTGAG TCATGGATTT CCTCCTATAC ACCAGCCTCT TCTGGCAGAG 
GACCCCACAG CTGCTGATTT CAAGCAGGAA CTCAGGCGGA AAAGTAAATT GGTGGAAGAG 
CCAATAGACA TGGATTCTCC AGAAATCAGA GAACTTGAAA AGTTTGCCAA TGAATTTAAA 
GTGAGACGAA TTAAATTAGG ATACACCCAG ACAAATGTTG GGGAGGCCCT GGCAGCTGTG 
CATGGCTCTG AATTCAGTCA AACAACAATC TGCCGATTTG AAAATCTGCA GCTCAGCTTT 
AAAAATGCAT GCAAACTGAA AGCAATATTA TCCAAATGGC TGGAGGAAGC TGAGCAAGTA 
GGAGCTTTGT ACAATGAAAA AGTGGGAGCA AATGAAAGGA AAAGAAAACG AAGAACAACT 
ATAAGCATTG CTGCTAAAGA TGCTCTGGAG AGACACTTTG GAGAACAGAA TAAACCTTCT 
TCTCAAGAGA TCATGAGGAT GGCTGAAGAA CTGAATCTGG AGAAAGAAGT AGTAAGAGTT 
TGGTTTTGCA ACCGGAGGCA GAGAGAAAAA CGGGTGAAAA CAAGTCTGAA TCAGAGTTTA 
TTTTCTATTT CTAAGGAACA TCTTGAGTGC AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000297.1
CDS	123..998
Translation	MSCQAFTSADTFIPLNSDASATLPLIMHHSAAECLPVSNHATNVMSTATGLHYSVPSCHYGNQPSTYGVMAGSLTPCLYKFPDHTLSHGFPPIHQPLLAEDPTAADFKQELRRKSKLVEEPIDMDSPEIRELEKFANEFKVRRIKLGYTQTNVGEALAAVHGSEFSQTTICRFENLQLSFKNACKLKAILSKWLEEAEQVGALYNEKVGANERKRKRRTTISIAAKDALERHFGEQNKPSSQEIMRMAEELNLEKEVVRVWFCNRRQREKRVKTSLNQSLFSISKEHLECR

Target ORF information:

RefSeq Version	NM_000306.4
Organism	Homo sapiens(human)
Definition	Homo sapiens POU class 1 homeobox 1 (POU1F1), transcript variant alpha, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000306.4

ORF Insert Sequence:

ATGAGTTGCC AAGCTTTTAC TTCGGCTGAT ACCTTTATAC CTCTGAATTC TGACGCCTCT 
GCAACTCTGC CTCTGATAAT GCATCACAGT GCTGCCGAGT GTCTACCAGT CTCCAACCAT 
GCCACCAATG TGATGTCTAC AGCAACAGGA CTTCATTATT CTGTTCCTTC CTGTCATTAT 
GGAAACCAGC CATCAACCTA TGGAGTGATG GCAGGTAGTT TAACCCCTTG TCTTTATAAA 
TTTCCTGACC ACACCTTGAG TCATGGATTT CCTCCTATAC ACCAGCCTCT TCTGGCAGAG 
GACCCCACAG CTGCTGATTT CAAGCAGGAA CTCAGGCGGA AAAGTAAATT GGTGGAAGAG 
CCAATAGACA TGGATTCTCC AGAAATCAGA GAACTTGAAA AGTTTGCCAA TGAATTTAAA 
GTGAGACGAA TTAAATTAGG ATACACCCAG ACAAATGTTG GGGAGGCCCT GGCAGCTGTG 
CATGGCTCTG AATTCAGTCA AACAACAATC TGCCGATTTG AAAATCTGCA GCTCAGCTTT 
AAAAATGCAT GCAAACTGAA AGCAATATTA TCCAAATGGC TGGAGGAAGC TGAGCAAGTA 
GGAGCTTTGT ACAATGAAAA AGTGGGAGCA AATGAAAGGA AAAGAAAACG AAGAACAACT 
ATAAGCATTG CTGCTAAAGA TGCTCTGGAG AGACACTTTG GAGAACAGAA TAAACCTTCT 
TCTCAAGAGA TCATGAGGAT GGCTGAAGAA CTGAATCTGG AGAAAGAAGT AGTAAGAGTT 
TGGTTTTGCA ACCGGAGGCA GAGAGAAAAA CGGGTGAAAA CAAGTCTGAA TCAGAGTTTA 
TTTTCTATTT CTAAGGAACA TCTTGAGTGC AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Biochemical and structural characterization of a novel cooperative binding mode by Pit-1 with CATT repeats in the macrophage migration inhibitory factor promoter.
Nucleic acids research(2017 Nov)
Agarwal S,Cho TY

Combined pituitary hormone deficiency due to gross deletions in the POU1F1 (PIT-1) and PROP1 genes.
Journal of human genetics62(8)755-762(2017 Aug)
Bertko E,Klammt J,Dusatkova P,Bahceci M,Gonc N,Ten Have L,Kandemir N,Mansmann G,Obermannova B,Oostdijk W,Pf?ffle H,Rockstroh-Lippold D,Schlicke M,Tuzcu AK,Pf?ffle R

A novel heterozygous intronic mutation in POU1F1 is associated with combined pituitary hormone deficiency.
Endocrine journal64(2)229-234(2017 Feb)
Takagi M,Kamasaki H,Yagi H,Fukuzawa R,Narumi S,Hasegawa T

Over-Expression of POU Class 1 Homeobox 1 Transcription Factor (Pit-1) Predicts Poor Prognosis for Breast Cancer Patients.
Medical science monitor : international medical journal of experimental and clinical research224121-4125(2016 Oct)
Gao Z,Xue K,Zhang L,Wei M

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POU1F1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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