ETAA1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ETAA1
Entrez Gene ID	54465
Full Name	ETAA1, ATR kinase activator
Synonyms	ETAA16
General protein information	Preferred Names ETAA1, ATR kinase activator Names ewing's tumor-associated antigen 1 Ewing tumor associated antigen 1 ewing's tumor-associated antigen 16
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p14
Disorder MIM:	613196

mRNA and Protein(s)

mRNA	Protein	Name
XM_017004377.1	XP_016859866.1	ewing's tumor-associated antigen 1 isoform X2
NM_019002.3	NP_061875.2	ewing's tumor-associated antigen 1
NM_019002.4	NP_061875.2	ewing's tumor-associated antigen 1
XM_017004376.1	XP_016859865.1	ewing's tumor-associated antigen 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ETAA1	XP_001167176.1
Bos taurus (cattle)	ETAA1	NP_001068596.1
Rattus norvegicus (Norway rat)	LOC316820	XP_229242.2
	ETAA1	XP_001093331.1
Canis lupus familiaris (dog)	ETAA1	XP_531851.2
Mus musculus (house mouse)	Etaa1	NP_080852.2
Xenopus tropicalis (tropical clawed frog)	etaa1	NP_001072276.1
Homo sapiens (human)	ETAA1	NP_061875.2
Rattus norvegicus (Norway rat)	Etaa1	NP_001102564.1

Gene Ontology
Bibliography

Related articles in PubMed

DNA Replication Checkpoint: New ATR Activator Identified.
Zou L
Current biology : CB27(1)R33-R35(2017 Jan)

RPA-Binding Protein ETAA1 Is an ATR Activator Involved in DNA Replication Stress Response.
Lee YC, Zhou Q, Chen J, Yuan J
Current biology : CB26(24)3257-3268(2016 Dec)

ETAA1 acts at stalled replication forks to maintain genome integrity.
Bass TE, Luzwick JW, Kavanaugh G, Carroll C, Dungrawala H, Glick GG, Feldkamp MD, Putney R, Chazin WJ, Cortez D
Nature cell biology18(11)1185-1195(2016 Nov)

Activation of the ATR kinase by the RPA-binding protein ETAA1.
Haahr P, Hoffmann S, Tollenaere MA, Ho T, Toledo LI, Mann M, Bekker-Jensen S, R?schle M, Mailand N
Nature cell biology18(11)1196-1207(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ETAA1 acts as a direct ATR serine-threonine kinase protein activator
Title: ETAA1 acts at stalled replication forks to maintain genome integrity.

that parallel TopBP1- and ETAA1-mediated pathways underlie ATR activation and that their combined action is essential for coping with replication stress
Title: Activation of the ATR kinase by the RPA-binding protein ETAA1.

results reveal that ETAA1 is a novel RPA-interacting protein that promotes restart of stalled replication forks.
Title: Ewing Tumor-associated Antigen 1 Interacts with Replication Protein A to Promote Restart of Stalled Replication Forks.

results suggest that ETAA16 may function as a tumour-specific cell surface antigen in Ewing's family of tumors
Title: Structure and function of ETAA16: a novel cell surface antigen in Ewing's tumours.

The following ETAA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ETAA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13511	NM_019002.4 Latest version!	Homo sapiens ETAA1 activator of ATR kinase (ETAA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu89592	XM_017004377.1 Latest version!	Homo sapiens ETAA1 activator of ATR kinase (ETAA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu89592	XM_017004376.1 Latest version!	Homo sapiens ETAA1 activator of ATR kinase (ETAA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OHu13511	NM_019002.3	Homo sapiens ETAA1 activator of ATR kinase (ETAA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ETAA1 ( NM_019002.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_061875.2 Homo sapiens ETAA1 activator of ATR kinase (ETAA1), mRNA.

Price & Availability↑

CloneID	OHu13511
Clone ID Related Accession (Same CDS sequence)	NM_019002.3 , NM_019002.4
Accession Version	NM_019002.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	ewing's tumor-associated antigen 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AW978869.1, BC033075.1, BC040001.1 and AJ242682.2. On Mar 7, 2006 this sequence version replaced NM_019002.2. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.142381.1, AJ242682.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGAGTCGGC GAAGGAAACA TGATGACAGC CCTAGCCCGA AGAAAACGCC GCACAAAACA 
GTGGCGGCGG AGGAATGCGG CTCGGTGGTC GAGCCAGGGA GGAGGCGGCT GAGATCGGCC 
CGCGGTTCGT GGCCCTGCGG GGCTAGAGAG GGGCCTCCCG GGCCAGTGCG GCAGCGAGAG 
CAGCCTCCGA CCGCCGCCCT GTGCAGTAAA AGTAACCCCG AGGAAAGGTA TGAAACACCA 
AAGAGAGCGC TGAAAATGGA CTCACTGTCA TCTTCCTTCA GTTCTCCTAA TGATCCAGAT 
GGACAGAATG ATATCTTTTG GGATCAGAAT TCTCCATTGA CAAAGCAGTT AGGTAAAGGA 
AGAAAAAAAC AGATTTACAC CACAGATAGT GATGAGATTT CACATATTGT TAATCGTATT 
GCTCCTCAGG ATGAAAAACC AACAACAAAT TCTATGCTGG ACATGTGGAT TGGTGAAACT 
GCTATTCCTT GTACTCCCAG TGTAGCAAAA GGAAAATCAA GAGCAAAAAT CAGCTGCACA 
AAGTTAAAAA CACAAAGTCA AGAAGAAGAA CTTATGAAAC TGGCTAAACA ATTTGATAAA 
AATATGGAAG AGCTAGATGT GATTCAAGAG CAAAACAAGA GGAATTATGA TTTTACCCAG 
ATGATTTCAG AAACAGAGAT TTTAAGTAAT TATAAAGATA ATATACAGAT GTGGTCATTA 
CATAATATAG TTCCCGAAAT AGATAATGCT ACAAAAAAGC CAATCAAAGG AAACACCAAG 
ATATCTGTGG CAAATAATCA AAATAGCAGT CAGAAGCCAT TTGACCAAAT TGCTGAAGCA 
GCCTTTAATG CTATTTTTGA TGGTTCTACT CAGAAATGTA GCGGACAGTT AAGCCAAGAA 
CTGCCAGAGG CTTTTTGGAG CACCAGTAAT ACTACCTTTG TAAAGACAAA TGCTTTGAAA 
GAGGAGAAAA TCATTACTAA TGAAACTCTG GTCATTGAAA AACTGTCAAA TAAAACCCCA 
CGATCACTTT CTTCTCAAGT AGATACACCC ATAATGACAA AATCATGTGT GACTTCCTGT 
ACTAAGGAGC CAGAAACTTC TAATAAGTAC ATTGATGCAT TTACTACAAG TGATTTTGAG 
GATGATTGGG AAAACTTACT AGGTAGTGAA CCTTTTGCTA TGCAAAATAT CGACATGCCT 
GAACTCTTTC CTTCTAAAAC AGCCCATGTT ACTGATCAAA AGGAAATTTG TACCTTTAAT 
AGTAAAACTG TTAAAAATAC GTCAAGAGCA AATACAAGTC CAGATGCCAG GTTAGGAGAT 
TCAAAAGTAT TACAAGATCT TTCTTCAAAG ACATATGACA GAGAATTAAT AGATGCAGAA 
TATAGATTTT CACCAAATTC AAATAAATCA AACAAATTAT CCACTGGAAA TAAAATGAAA 
TTTGAGAACT CTTCCAATAA AATTGTTATT CAAGACGAAA TTCAAAATTG TATAGTTACA 
TCTAATCTGA CAAAAATAAA GGAAGATATT CTTACTAACT CTACTGAAGC TTCTGAAAGG 
AAGTCAGCTT TGAACACAAG GTATTCTAAT GAACAGAAAA ATAAGTGCAT TTTAAATCAG 
TCTATTAAAG CCCCTGTTAA TACTGATCTT TTTGGCTCTG CAAATCTAGG CAGTAAAACC 
AGTGTTAGTA ACCCAAATCA GACTAGTGCA TCAAAAGTAG GTTCTTTCTT TGATGATTGG 
AATGATCCCT CATTTGCCAA TGAAATTATT AAAGCATGTC ATCAATTAGA TAATACCTGG 
GAAGCAGATG ATGTAGATGA TGATTTGTTG TACCAAGCAT GTGATGATAT TGAAAGACTA 
ACTCAGCAAC AAGACATTAG AAAGGACAGT AAGACATCAG AAAGTATATG TGAGATCAAT 
AATAATTCCG AACATGGAGC CAAACTAACT CAGCAACAAG ACATTAGAAA GGACAGTAAG 
ACATCAGAAA GTATATGTGA GATCAATAAT AATTCCGAAC ATGGAGCCAA AAACATGTTT 
GCTATATCTA AACAAGGAAG TAATTTGGTA CAATCAAAGC ATTTGAATCC AGGCAGCATT 
TCAGTGCAGA CATCTTTGAC AAATAGCTCA CAAATAGATA AGCCAATGAA GATGGAGAAA 
GGGGAAATGT ATGGAAATTC TCCAAGATTT TTAGGTGCCA CAAATTTGAC TATGTATTCT 
AAGATCTCAA ACTGTCAGAT AAATAATCTG CATGTGTCTT ATACTAACAC TGATGTTCCA 
ATACAAGTGA ATAGTTCCAA ATTGGTTCTT CCAGGAAGTT CAAGTTTGAA TGTAACTTCA 
GATCATATGA ATACAGAAAT TACTACTTAT AAGAAGAAAT TGAGTACTAA TCAGCCATGC 
CATAAGACTG TAACAGATGA AGCTCAGAGC AACCTTAACA CAACAGTTGG ATTTTCAAAG 
TTTACATTTA CAAGGATGAA AAATTCTCAG ATTCTTTCTC AGTTTAATCA AAATTGTATA 
ACTGGAAGTA TGTCTGATAC CAAAATTACA CAGGGTGTGG AGAAAAAGAA AGGTGTCAAC 
CCATTACTGG AGGAAGCTGT TGGACAGCAA TCTTTGGTGA AACTTTCTGA ATCTTTGAAA 
CAATCTTCAA AAGAGGAAGA AGAGAAAAAT AGAAAGTGTT CTCCTGAAGA AATTCAGAGA 
AAAAGACAAG AAGCACTGGT TCGGAGAATG GCTAAAGCAC GAGCCTCATC TGTAAATGCA 
GCTCCCACTT CATTTCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061875.2
CDS	140..2920
Translation	MSRRRKHDDSPSPKKTPHKTVAAEECGSVVEPGRRRLRSARGSWPCGAREGPPGPVRQREQPPTAALCSKSNPEERYETPKRALKMDSLSSSFSSPNDPDGQNDIFWDQNSPLTKQLGKGRKKQIYTTDSDEISHIVNRIAPQDEKPTTNSMLDMWIGETAIPCTPSVAKGKSRAKISCTKLKTQSQEEELMKLAKQFDKNMEELDVIQEQNKRNYDFTQMISETEILSNYKDNIQMWSLHNIVPEIDNATKKPIKGNTKISVANNQNSSQKPFDQIAEAAFNAIFDGSTQKCSGQLSQELPEAFWSTSNTTFVKTNALKEEKIITNETLVIEKLSNKTPRSLSSQVDTPIMTKSCVTSCTKEPETSNKYIDAFTTSDFEDDWENLLGSEPFAMQNIDMPELFPSKTAHVTDQKEICTFNSKTVKNTSRANTSPDARLGDSKVLQDLSSKTYDRELIDAEYRFSPNSNKSNKLSTGNKMKFENSSNKIVIQDEIQNCIVTSNLTKIKEDILTNSTEASERKSALNTRYSNEQKNKCILNQSIKAPVNTDLFGSANLGSKTSVSNPNQTSASKVGSFFDDWNDPSFANEIIKACHQLDNTWEADDVDDDLLYQACDDIERLTQQQDIRKDSKTSESICEINNNSEHGAKLTQQQDIRKDSKTSESICEINNNSEHGAKNMFAISKQGSNLVQSKHLNPGSISVQTSLTNSSQIDKPMKMEKGEMYGNSPRFLGATNLTMYSKISNCQINNLHVSYTNTDVPIQVNSSKLVLPGSSSLNVTSDHMNTEITTYKKKLSTNQPCHKTVTDEAQSNLNTTVGFSKFTFTRMKNSQILSQFNQNCITGSMSDTKITQGVEKKKGVNPLLEEAVGQQSLVKLSESLKQSSKEEEEKNRKCSPEEIQRKRQEALVRRMAKARASSVNAAPTSFL

Target ORF information:

RefSeq Version	NM_019002.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ETAA1 activator of ATR kinase (ETAA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019002.3

ORF Insert Sequence:

ATGAGTCGGC GAAGGAAACA TGATGACAGC CCTAGCCCGA AGAAAACGCC GCACAAAACA 
GTGGCGGCGG AGGAATGCGG CTCGGTGGTC GAGCCAGGGA GGAGGCGGCT GAGATCGGCC 
CGCGGTTCGT GGCCCTGCGG GGCTAGAGAG GGGCCTCCCG GGCCAGTGCG GCAGCGAGAG 
CAGCCTCCGA CCGCCGCCCT GTGCAGTAAA AGTAACCCCG AGGAAAGGTA TGAAACACCA 
AAGAGAGCGC TGAAAATGGA CTCACTGTCA TCTTCCTTCA GTTCTCCTAA TGATCCAGAT 
GGACAGAATG ATATCTTTTG GGATCAGAAT TCTCCATTGA CAAAGCAGTT AGGTAAAGGA 
AGAAAAAAAC AGATTTACAC CACAGATAGT GATGAGATTT CACATATTGT TAATCGTATT 
GCTCCTCAGG ATGAAAAACC AACAACAAAT TCTATGCTGG ACATGTGGAT TGGTGAAACT 
GCTATTCCTT GTACTCCCAG TGTAGCAAAA GGAAAATCAA GAGCAAAAAT CAGCTGCACA 
AAGTTAAAAA CACAAAGTCA AGAAGAAGAA CTTATGAAAC TGGCTAAACA ATTTGATAAA 
AATATGGAAG AGCTAGATGT GATTCAAGAG CAAAACAAGA GGAATTATGA TTTTACCCAG 
ATGATTTCAG AAACAGAGAT TTTAAGTAAT TATAAAGATA ATATACAGAT GTGGTCATTA 
CATAATATAG TTCCCGAAAT AGATAATGCT ACAAAAAAGC CAATCAAAGG AAACACCAAG 
ATATCTGTGG CAAATAATCA AAATAGCAGT CAGAAGCCAT TTGACCAAAT TGCTGAAGCA 
GCCTTTAATG CTATTTTTGA TGGTTCTACT CAGAAATGTA GCGGACAGTT AAGCCAAGAA 
CTGCCAGAGG CTTTTTGGAG CACCAGTAAT ACTACCTTTG TAAAGACAAA TGCTTTGAAA 
GAGGAGAAAA TCATTACTAA TGAAACTCTG GTCATTGAAA AACTGTCAAA TAAAACCCCA 
CGATCACTTT CTTCTCAAGT AGATACACCC ATAATGACAA AATCATGTGT GACTTCCTGT 
ACTAAGGAGC CAGAAACTTC TAATAAGTAC ATTGATGCAT TTACTACAAG TGATTTTGAG 
GATGATTGGG AAAACTTACT AGGTAGTGAA CCTTTTGCTA TGCAAAATAT CGACATGCCT 
GAACTCTTTC CTTCTAAAAC AGCCCATGTT ACTGATCAAA AGGAAATTTG TACCTTTAAT 
AGTAAAACTG TTAAAAATAC GTCAAGAGCA AATACAAGTC CAGATGCCAG GTTAGGAGAT 
TCAAAAGTAT TACAAGATCT TTCTTCAAAG ACATATGACA GAGAATTAAT AGATGCAGAA 
TATAGATTTT CACCAAATTC AAATAAATCA AACAAATTAT CCACTGGAAA TAAAATGAAA 
TTTGAGAACT CTTCCAATAA AATTGTTATT CAAGACGAAA TTCAAAATTG TATAGTTACA 
TCTAATCTGA CAAAAATAAA GGAAGATATT CTTACTAACT CTACTGAAGC TTCTGAAAGG 
AAGTCAGCTT TGAACACAAG GTATTCTAAT GAACAGAAAA ATAAGTGCAT TTTAAATCAG 
TCTATTAAAG CCCCTGTTAA TACTGATCTT TTTGGCTCTG CAAATCTAGG CAGTAAAACC 
AGTGTTAGTA ACCCAAATCA GACTAGTGCA TCAAAAGTAG GTTCTTTCTT TGATGATTGG 
AATGATCCCT CATTTGCCAA TGAAATTATT AAAGCATGTC ATCAATTAGA TAATACCTGG 
GAAGCAGATG ATGTAGATGA TGATTTGTTG TACCAAGCAT GTGATGATAT TGAAAGACTA 
ACTCAGCAAC AAGACATTAG AAAGGACAGT AAGACATCAG AAAGTATATG TGAGATCAAT 
AATAATTCCG AACATGGAGC CAAACTAACT CAGCAACAAG ACATTAGAAA GGACAGTAAG 
ACATCAGAAA GTATATGTGA GATCAATAAT AATTCCGAAC ATGGAGCCAA AAACATGTTT 
GCTATATCTA AACAAGGAAG TAATTTGGTA CAATCAAAGC ATTTGAATCC AGGCAGCATT 
TCAGTGCAGA CATCTTTGAC AAATAGCTCA CAAATAGATA AGCCAATGAA GATGGAGAAA 
GGGGAAATGT ATGGAAATTC TCCAAGATTT TTAGGTGCCA CAAATTTGAC TATGTATTCT 
AAGATCTCAA ACTGTCAGAT AAATAATCTG CATGTGTCTT ATACTAACAC TGATGTTCCA 
ATACAAGTGA ATAGTTCCAA ATTGGTTCTT CCAGGAAGTT CAAGTTTGAA TGTAACTTCA 
GATCATATGA ATACAGAAAT TACTACTTAT AAGAAGAAAT TGAGTACTAA TCAGCCATGC 
CATAAGACTG TAACAGATGA AGCTCAGAGC AACCTTAACA CAACAGTTGG ATTTTCAAAG 
TTTACATTTA CAAGGATGAA AAATTCTCAG ATTCTTTCTC AGTTTAATCA AAATTGTATA 
ACTGGAAGTA TGTCTGATAC CAAAATTACA CAGGGTGTGG AGAAAAAGAA AGGTGTCAAC 
CCATTACTGG AGGAAGCTGT TGGACAGCAA TCTTTGGTGA AACTTTCTGA ATCTTTGAAA 
CAATCTTCAA AAGAGGAAGA AGAGAAAAAT AGAAAGTGTT CTCCTGAAGA AATTCAGAGA 
AAAAGACAAG AAGCACTGGT TCGGAGAATG GCTAAAGCAC GAGCCTCATC TGTAAATGCA 
GCTCCCACTT CATTTCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ETAA1 ( NM_019002.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_061875.2 Homo sapiens ETAA1 activator of ATR kinase (ETAA1), mRNA.

Price & Availability↑

CloneID	OHu13511
Clone ID Related Accession (Same CDS sequence)	NM_019002.3 , NM_019002.4
Accession Version	NM_019002.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	ewing's tumor-associated antigen 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AW978869.1, BC033075.1, BC040001.1, AJ242682.2 and AC023668.5. On May 17, 2019 this sequence version replaced NM_019002.3. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.75905.1, SRR1803614.16087.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000272342.6/ ENSP00000272342.5 RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END##

ATGAGTCGGC GAAGGAAACA TGATGACAGC CCTAGCCCGA AGAAAACGCC GCACAAAACA 
GTGGCGGCGG AGGAATGCGG CTCGGTGGTC GAGCCAGGGA GGAGGCGGCT GAGATCGGCC 
CGCGGTTCGT GGCCCTGCGG GGCTAGAGAG GGGCCTCCCG GGCCAGTGCG GCAGCGAGAG 
CAGCCTCCGA CCGCCGCCCT GTGCAGTAAA AGTAACCCCG AGGAAAGGTA TGAAACACCA 
AAGAGAGCGC TGAAAATGGA CTCACTGTCA TCTTCCTTCA GTTCTCCTAA TGATCCAGAT 
GGACAGAATG ATATCTTTTG GGATCAGAAT TCTCCATTGA CAAAGCAGTT AGGTAAAGGA 
AGAAAAAAAC AGATTTACAC CACAGATAGT GATGAGATTT CACATATTGT TAATCGTATT 
GCTCCTCAGG ATGAAAAACC AACAACAAAT TCTATGCTGG ACATGTGGAT TGGTGAAACT 
GCTATTCCTT GTACTCCCAG TGTAGCAAAA GGAAAATCAA GAGCAAAAAT CAGCTGCACA 
AAGTTAAAAA CACAAAGTCA AGAAGAAGAA CTTATGAAAC TGGCTAAACA ATTTGATAAA 
AATATGGAAG AGCTAGATGT GATTCAAGAG CAAAACAAGA GGAATTATGA TTTTACCCAG 
ATGATTTCAG AAACAGAGAT TTTAAGTAAT TATAAAGATA ATATACAGAT GTGGTCATTA 
CATAATATAG TTCCCGAAAT AGATAATGCT ACAAAAAAGC CAATCAAAGG AAACACCAAG 
ATATCTGTGG CAAATAATCA AAATAGCAGT CAGAAGCCAT TTGACCAAAT TGCTGAAGCA 
GCCTTTAATG CTATTTTTGA TGGTTCTACT CAGAAATGTA GCGGACAGTT AAGCCAAGAA 
CTGCCAGAGG CTTTTTGGAG CACCAGTAAT ACTACCTTTG TAAAGACAAA TGCTTTGAAA 
GAGGAGAAAA TCATTACTAA TGAAACTCTG GTCATTGAAA AACTGTCAAA TAAAACCCCA 
CGATCACTTT CTTCTCAAGT AGATACACCC ATAATGACAA AATCATGTGT GACTTCCTGT 
ACTAAGGAGC CAGAAACTTC TAATAAGTAC ATTGATGCAT TTACTACAAG TGATTTTGAG 
GATGATTGGG AAAACTTACT AGGTAGTGAA CCTTTTGCTA TGCAAAATAT CGACATGCCT 
GAACTCTTTC CTTCTAAAAC AGCCCATGTT ACTGATCAAA AGGAAATTTG TACCTTTAAT 
AGTAAAACTG TTAAAAATAC GTCAAGAGCA AATACAAGTC CAGATGCCAG GTTAGGAGAT 
TCAAAAGTAT TACAAGATCT TTCTTCAAAG ACATATGACA GAGAATTAAT AGATGCAGAA 
TATAGATTTT CACCAAATTC AAATAAATCA AACAAATTAT CCACTGGAAA TAAAATGAAA 
TTTGAGAACT CTTCCAATAA AATTGTTATT CAAGACGAAA TTCAAAATTG TATAGTTACA 
TCTAATCTGA CAAAAATAAA GGAAGATATT CTTACTAACT CTACTGAAGC TTCTGAAAGG 
AAGTCAGCTT TGAACACAAG GTATTCTAAT GAACAGAAAA ATAAGTGCAT TTTAAATCAG 
TCTATTAAAG CCCCTGTTAA TACTGATCTT TTTGGCTCTG CAAATCTAGG CAGTAAAACC 
AGTGTTAGTA ACCCAAATCA GACTAGTGCA TCAAAAGTAG GTTCTTTCTT TGATGATTGG 
AATGATCCCT CATTTGCCAA TGAAATTATT AAAGCATGTC ATCAATTAGA TAATACCTGG 
GAAGCAGATG ATGTAGATGA TGATTTGTTG TACCAAGCAT GTGATGATAT TGAAAGACTA 
ACTCAGCAAC AAGACATTAG AAAGGACAGT AAGACATCAG AAAGTATATG TGAGATCAAT 
AATAATTCCG AACATGGAGC CAAACTAACT CAGCAACAAG ACATTAGAAA GGACAGTAAG 
ACATCAGAAA GTATATGTGA GATCAATAAT AATTCCGAAC ATGGAGCCAA AAACATGTTT 
GCTATATCTA AACAAGGAAG TAATTTGGTA CAATCAAAGC ATTTGAATCC AGGCAGCATT 
TCAGTGCAGA CATCTTTGAC AAATAGCTCA CAAATAGATA AGCCAATGAA GATGGAGAAA 
GGGGAAATGT ATGGAAATTC TCCAAGATTT TTAGGTGCCA CAAATTTGAC TATGTATTCT 
AAGATCTCAA ACTGTCAGAT AAATAATCTG CATGTGTCTT ATACTAACAC TGATGTTCCA 
ATACAAGTGA ATAGTTCCAA ATTGGTTCTT CCAGGAAGTT CAAGTTTGAA TGTAACTTCA 
GATCATATGA ATACAGAAAT TACTACTTAT AAGAAGAAAT TGAGTACTAA TCAGCCATGC 
CATAAGACTG TAACAGATGA AGCTCAGAGC AACCTTAACA CAACAGTTGG ATTTTCAAAG 
TTTACATTTA CAAGGATGAA AAATTCTCAG ATTCTTTCTC AGTTTAATCA AAATTGTATA 
ACTGGAAGTA TGTCTGATAC CAAAATTACA CAGGGTGTGG AGAAAAAGAA AGGTGTCAAC 
CCATTACTGG AGGAAGCTGT TGGACAGCAA TCTTTGGTGA AACTTTCTGA ATCTTTGAAA 
CAATCTTCAA AAGAGGAAGA AGAGAAAAAT AGAAAGTGTT CTCCTGAAGA AATTCAGAGA 
AAAAGACAAG AAGCACTGGT TCGGAGAATG GCTAAAGCAC GAGCCTCATC TGTAAATGCA 
GCTCCCACTT CATTTCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061875.2
CDS	117..2897
Translation	MSRRRKHDDSPSPKKTPHKTVAAEECGSVVEPGRRRLRSARGSWPCGAREGPPGPVRQREQPPTAALCSKSNPEERYETPKRALKMDSLSSSFSSPNDPDGQNDIFWDQNSPLTKQLGKGRKKQIYTTDSDEISHIVNRIAPQDEKPTTNSMLDMWIGETAIPCTPSVAKGKSRAKISCTKLKTQSQEEELMKLAKQFDKNMEELDVIQEQNKRNYDFTQMISETEILSNYKDNIQMWSLHNIVPEIDNATKKPIKGNTKISVANNQNSSQKPFDQIAEAAFNAIFDGSTQKCSGQLSQELPEAFWSTSNTTFVKTNALKEEKIITNETLVIEKLSNKTPRSLSSQVDTPIMTKSCVTSCTKEPETSNKYIDAFTTSDFEDDWENLLGSEPFAMQNIDMPELFPSKTAHVTDQKEICTFNSKTVKNTSRANTSPDARLGDSKVLQDLSSKTYDRELIDAEYRFSPNSNKSNKLSTGNKMKFENSSNKIVIQDEIQNCIVTSNLTKIKEDILTNSTEASERKSALNTRYSNEQKNKCILNQSIKAPVNTDLFGSANLGSKTSVSNPNQTSASKVGSFFDDWNDPSFANEIIKACHQLDNTWEADDVDDDLLYQACDDIERLTQQQDIRKDSKTSESICEINNNSEHGAKLTQQQDIRKDSKTSESICEINNNSEHGAKNMFAISKQGSNLVQSKHLNPGSISVQTSLTNSSQIDKPMKMEKGEMYGNSPRFLGATNLTMYSKISNCQINNLHVSYTNTDVPIQVNSSKLVLPGSSSLNVTSDHMNTEITTYKKKLSTNQPCHKTVTDEAQSNLNTTVGFSKFTFTRMKNSQILSQFNQNCITGSMSDTKITQGVEKKKGVNPLLEEAVGQQSLVKLSESLKQSSKEEEEKNRKCSPEEIQRKRQEALVRRMAKARASSVNAAPTSFL

Target ORF information:

RefSeq Version	NM_019002.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ETAA1 activator of ATR kinase (ETAA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019002.4

ORF Insert Sequence:

ATGAGTCGGC GAAGGAAACA TGATGACAGC CCTAGCCCGA AGAAAACGCC GCACAAAACA 
GTGGCGGCGG AGGAATGCGG CTCGGTGGTC GAGCCAGGGA GGAGGCGGCT GAGATCGGCC 
CGCGGTTCGT GGCCCTGCGG GGCTAGAGAG GGGCCTCCCG GGCCAGTGCG GCAGCGAGAG 
CAGCCTCCGA CCGCCGCCCT GTGCAGTAAA AGTAACCCCG AGGAAAGGTA TGAAACACCA 
AAGAGAGCGC TGAAAATGGA CTCACTGTCA TCTTCCTTCA GTTCTCCTAA TGATCCAGAT 
GGACAGAATG ATATCTTTTG GGATCAGAAT TCTCCATTGA CAAAGCAGTT AGGTAAAGGA 
AGAAAAAAAC AGATTTACAC CACAGATAGT GATGAGATTT CACATATTGT TAATCGTATT 
GCTCCTCAGG ATGAAAAACC AACAACAAAT TCTATGCTGG ACATGTGGAT TGGTGAAACT 
GCTATTCCTT GTACTCCCAG TGTAGCAAAA GGAAAATCAA GAGCAAAAAT CAGCTGCACA 
AAGTTAAAAA CACAAAGTCA AGAAGAAGAA CTTATGAAAC TGGCTAAACA ATTTGATAAA 
AATATGGAAG AGCTAGATGT GATTCAAGAG CAAAACAAGA GGAATTATGA TTTTACCCAG 
ATGATTTCAG AAACAGAGAT TTTAAGTAAT TATAAAGATA ATATACAGAT GTGGTCATTA 
CATAATATAG TTCCCGAAAT AGATAATGCT ACAAAAAAGC CAATCAAAGG AAACACCAAG 
ATATCTGTGG CAAATAATCA AAATAGCAGT CAGAAGCCAT TTGACCAAAT TGCTGAAGCA 
GCCTTTAATG CTATTTTTGA TGGTTCTACT CAGAAATGTA GCGGACAGTT AAGCCAAGAA 
CTGCCAGAGG CTTTTTGGAG CACCAGTAAT ACTACCTTTG TAAAGACAAA TGCTTTGAAA 
GAGGAGAAAA TCATTACTAA TGAAACTCTG GTCATTGAAA AACTGTCAAA TAAAACCCCA 
CGATCACTTT CTTCTCAAGT AGATACACCC ATAATGACAA AATCATGTGT GACTTCCTGT 
ACTAAGGAGC CAGAAACTTC TAATAAGTAC ATTGATGCAT TTACTACAAG TGATTTTGAG 
GATGATTGGG AAAACTTACT AGGTAGTGAA CCTTTTGCTA TGCAAAATAT CGACATGCCT 
GAACTCTTTC CTTCTAAAAC AGCCCATGTT ACTGATCAAA AGGAAATTTG TACCTTTAAT 
AGTAAAACTG TTAAAAATAC GTCAAGAGCA AATACAAGTC CAGATGCCAG GTTAGGAGAT 
TCAAAAGTAT TACAAGATCT TTCTTCAAAG ACATATGACA GAGAATTAAT AGATGCAGAA 
TATAGATTTT CACCAAATTC AAATAAATCA AACAAATTAT CCACTGGAAA TAAAATGAAA 
TTTGAGAACT CTTCCAATAA AATTGTTATT CAAGACGAAA TTCAAAATTG TATAGTTACA 
TCTAATCTGA CAAAAATAAA GGAAGATATT CTTACTAACT CTACTGAAGC TTCTGAAAGG 
AAGTCAGCTT TGAACACAAG GTATTCTAAT GAACAGAAAA ATAAGTGCAT TTTAAATCAG 
TCTATTAAAG CCCCTGTTAA TACTGATCTT TTTGGCTCTG CAAATCTAGG CAGTAAAACC 
AGTGTTAGTA ACCCAAATCA GACTAGTGCA TCAAAAGTAG GTTCTTTCTT TGATGATTGG 
AATGATCCCT CATTTGCCAA TGAAATTATT AAAGCATGTC ATCAATTAGA TAATACCTGG 
GAAGCAGATG ATGTAGATGA TGATTTGTTG TACCAAGCAT GTGATGATAT TGAAAGACTA 
ACTCAGCAAC AAGACATTAG AAAGGACAGT AAGACATCAG AAAGTATATG TGAGATCAAT 
AATAATTCCG AACATGGAGC CAAACTAACT CAGCAACAAG ACATTAGAAA GGACAGTAAG 
ACATCAGAAA GTATATGTGA GATCAATAAT AATTCCGAAC ATGGAGCCAA AAACATGTTT 
GCTATATCTA AACAAGGAAG TAATTTGGTA CAATCAAAGC ATTTGAATCC AGGCAGCATT 
TCAGTGCAGA CATCTTTGAC AAATAGCTCA CAAATAGATA AGCCAATGAA GATGGAGAAA 
GGGGAAATGT ATGGAAATTC TCCAAGATTT TTAGGTGCCA CAAATTTGAC TATGTATTCT 
AAGATCTCAA ACTGTCAGAT AAATAATCTG CATGTGTCTT ATACTAACAC TGATGTTCCA 
ATACAAGTGA ATAGTTCCAA ATTGGTTCTT CCAGGAAGTT CAAGTTTGAA TGTAACTTCA 
GATCATATGA ATACAGAAAT TACTACTTAT AAGAAGAAAT TGAGTACTAA TCAGCCATGC 
CATAAGACTG TAACAGATGA AGCTCAGAGC AACCTTAACA CAACAGTTGG ATTTTCAAAG 
TTTACATTTA CAAGGATGAA AAATTCTCAG ATTCTTTCTC AGTTTAATCA AAATTGTATA 
ACTGGAAGTA TGTCTGATAC CAAAATTACA CAGGGTGTGG AGAAAAAGAA AGGTGTCAAC 
CCATTACTGG AGGAAGCTGT TGGACAGCAA TCTTTGGTGA AACTTTCTGA ATCTTTGAAA 
CAATCTTCAA AAGAGGAAGA AGAGAAAAAT AGAAAGTGTT CTCCTGAAGA AATTCAGAGA 
AAAAGACAAG AAGCACTGGT TCGGAGAATG GCTAAAGCAC GAGCCTCATC TGTAAATGCA 
GCTCCCACTT CATTTCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ETAA1 ( XM_017004377.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859866.1 Homo sapiens ETAA1 activator of ATR kinase (ETAA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu89592
Clone ID Related Accession (Same CDS sequence)	XM_017004377.1 , XM_017004376.1
Accession Version	XM_017004377.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ewing's tumor-associated antigen 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCGGC GAAGGAAACA TGATGACAGC CCTAGCCCGA AGAAAACGCC GCACAAAACA 
GTGGCGGCGG AGGAATGCGG CTCGGTGGTC GAGCCAGGGA GGAGGCGGCT GAGATCGGCC 
CGCGGTTCGT GGCCCTGCGG GGCTAGAGAG GGGCCTCCCG GGCCAGTGCG GCAGCGAGAG 
CAGCCTCCGA CCGCCGCCCT GTGCAGTAAA AGTAACCCCG AGGAAAGGTA TGAAACACCA 
AAGAGAGCGC TGAAAATGGA CTCACTGTCA TCTTCCTTCA GTTCTCCTAA TGATCCAGAT 
GGACAGAATG ATATCTTTTG GGATCAGAAT TCTCCATTGA CAAAGCAGTT AGGTAAAGGA 
AGAAAAAAAC AGATTTACAC CACAGATAGT GATGAGATTT CACATATTGT TAATCGTATT 
GCTCCTCAGG ATGAAAAACC AACAACAAAT TCTATGCTGG ACATGTGGAT TGGTGAAACT 
GCTATTCCTT GTACTCCCAG TGTAGCAAAA GGAAAATCAA GAGCAAAAAT CAGCTGCACA 
AAGTTAAAAA CACAAAGTCA AGAAGAAGAA CTTATGAAAC TGGCTAAACA ATTTGATAAA 
AATATGGAAG AGCTAGATGT GATTCAAGAG CAAAACAAGA GGAATTATGA TTTTACCCAG 
ATGATTTCAG AAACAGAGAT TTTAAGTAAT TATAAAGATA ATATACAGAT GTGGTCATTA 
CATAATATAG TTCCCGAAAT AGATAATGCT ACAAAAAAGC CAATCAAAGG AAACACCAAG 
ATATCTGTGG CAAATAATCA AAATAGCAGT CAGAAGCCAT TTGACCAAAT TGCTGAAGCA 
GCCTTTAATG CTATTTTTGA TGGTTCTACT CAGAAATGTA GCGGACAGTT AAGCCAAGAA 
CTGCCAGAGG CTTTTTGGAG CACCAGTAAT ACTACCTTTG TAAAGACAAA TGCTTTGAAA 
GAGGAGAAAA TCATTACTAA TGAAACTCTG GTCATTGAAA AACTGTCAAA TAAAACCCCA 
CGATCACTTT CTTCTCAAGT AGATACACCC ATAATGACAA AATCATGTGT GACTTCCTGT 
ACTAAGGAGC CAGAAACTTC TAATAAGTAC ATTGATGCAT TTACTACAAG TGATTTTGAG 
GATGATTGGG AAAACTTACT AGGTAGTGAA CCTTTTGCTA TGCAAAATAT CGACATGCCT 
GAACTCTTTC CTTCTAAAAC AGCCCATGTT ACTGATCAAA AGGAAATTTG TACCTTTAAT 
AGTAAAACTG TTAAAAATAC GTCAAGAGCA AATACAAGTC CAGATGCCAG GTTAGGAGAT 
TCAAAAGTAT TACAAGATCT TTCTTCAAAG ACATATGACA GAGAATTAAT AGATGCAGAA 
TATAGATTTT CACCAAATTC AAATAAATCA AACAAATTAT CCACTGGAAA TAAAATGAAA 
TTTGAGAACT CTTCCAATAA AATTGTTATT CAAGACGAAA TTCAAAATTG TATAGTTACA 
TCTAATCTGA CAAAAATAAA GGAAGATATT CTTACTAACT CTACTGAAGC TTCTGAAAGG 
AAGTCAGCTT TGAACACAAG GTATTCTAAT GAACAGAAAA ATAAGTGCAT TTTAAATCAG 
TCTATTAAAG CCCCTGTTAA TACTGATCTT TTTGGCTCTG CAAATCTAGG CAGTAAAACC 
AGTGTTAGTA ACCCAAATCA GACTAGTGCA TCAAAAGTAG GTTCTTTCTT TGATGATTGG 
AATGATCCCT CATTTGCCAA TGAAATTATT AAAGCATGTC ATCAATTAGA TAATACCTGG 
GAAGCAGATG ATGTAGATGA TGATTTGTTG TACCAAGCAT GTGATGATAT TGAAAGACTA 
ACTCAGCAAC AAGACATTAG AAAGGACAGT AAGACATCAG AAAGTATATG TGAGATCAAT 
AATAATTCCG AACATGGAGC CAAAAACATG TTTGCTATAT CTAAACAAGG AAGTAATTTG 
GTACAATCAA AGCATTTGAA TCCAGGCAGC ATTTCAGTGC AGACATCTTT GACAAATAGC 
TCACAAATAG ATAAGCCAAT GAAGATGGAG AAAGGGGAAA TGTATGGAAA TTCTCCAAGA 
TTTTTAGGTG CCACAAATTT GACTATGTAT TCTAAGATCT CAAACTGTCA GATAAATAAT 
CTGCATGTGT CTTATACTAA CACTGATGTT CCAATACAAG TGAATAGTTC CAAATTGGTT 
CTTCCAGGAA GTTCAAGTTT GAATGTAACT TCAGATCATA TGAATACAGA AATTACTACT 
TATAAGAAGA AATTGAGTAC TAATCAGCCA TGCCATAAGA CTGTAACAGA TGAAGCTCAG 
AGCAACCTTA ACACAACAGT TGGATTTTCA AAGTTTACAT TTACAAGGAT GAAAAATTCT 
CAGATTCTTT CTCAGTTTAA TCAAAATTGT ATAACTGGAA GTATGTCTGA TACCAAAATT 
ACACAGGGTG TGGAGAAAAA GAAAGGTGTC AACCCATTAC TGGAGGAAGC TGTTGGACAG 
CAATCTTTGG TGAAACTTTC TGAATCTTTG AAACAATCTT CAAAAGAGGA AGAAGAGAAA 
AATAGAAAGT GTTCTCCTGA AGAAATTCAG AGAAAAAGAC AAGAAGCACT GGTTCGGAGA 
ATGGCTAAAG CACGAGCCTC ATCTGTAAAT GCAGCTCCCA CTTCATTTCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859866.1
CDS	133..2826
Translation	MSRRRKHDDSPSPKKTPHKTVAAEECGSVVEPGRRRLRSARGSWPCGAREGPPGPVRQREQPPTAALCSKSNPEERYETPKRALKMDSLSSSFSSPNDPDGQNDIFWDQNSPLTKQLGKGRKKQIYTTDSDEISHIVNRIAPQDEKPTTNSMLDMWIGETAIPCTPSVAKGKSRAKISCTKLKTQSQEEELMKLAKQFDKNMEELDVIQEQNKRNYDFTQMISETEILSNYKDNIQMWSLHNIVPEIDNATKKPIKGNTKISVANNQNSSQKPFDQIAEAAFNAIFDGSTQKCSGQLSQELPEAFWSTSNTTFVKTNALKEEKIITNETLVIEKLSNKTPRSLSSQVDTPIMTKSCVTSCTKEPETSNKYIDAFTTSDFEDDWENLLGSEPFAMQNIDMPELFPSKTAHVTDQKEICTFNSKTVKNTSRANTSPDARLGDSKVLQDLSSKTYDRELIDAEYRFSPNSNKSNKLSTGNKMKFENSSNKIVIQDEIQNCIVTSNLTKIKEDILTNSTEASERKSALNTRYSNEQKNKCILNQSIKAPVNTDLFGSANLGSKTSVSNPNQTSASKVGSFFDDWNDPSFANEIIKACHQLDNTWEADDVDDDLLYQACDDIERLTQQQDIRKDSKTSESICEINNNSEHGAKNMFAISKQGSNLVQSKHLNPGSISVQTSLTNSSQIDKPMKMEKGEMYGNSPRFLGATNLTMYSKISNCQINNLHVSYTNTDVPIQVNSSKLVLPGSSSLNVTSDHMNTEITTYKKKLSTNQPCHKTVTDEAQSNLNTTVGFSKFTFTRMKNSQILSQFNQNCITGSMSDTKITQGVEKKKGVNPLLEEAVGQQSLVKLSESLKQSSKEEEEKNRKCSPEEIQRKRQEALVRRMAKARASSVNAAPTSFL

Target ORF information:

RefSeq Version	XM_017004377.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ETAA1 activator of ATR kinase (ETAA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004377.1

ORF Insert Sequence:

ATGAGTCGGC GAAGGAAACA TGATGACAGC CCTAGCCCGA AGAAAACGCC GCACAAAACA 
GTGGCGGCGG AGGAATGCGG CTCGGTGGTC GAGCCAGGGA GGAGGCGGCT GAGATCGGCC 
CGCGGTTCGT GGCCCTGCGG GGCTAGAGAG GGGCCTCCCG GGCCAGTGCG GCAGCGAGAG 
CAGCCTCCGA CCGCCGCCCT GTGCAGTAAA AGTAACCCCG AGGAAAGGTA TGAAACACCA 
AAGAGAGCGC TGAAAATGGA CTCACTGTCA TCTTCCTTCA GTTCTCCTAA TGATCCAGAT 
GGACAGAATG ATATCTTTTG GGATCAGAAT TCTCCATTGA CAAAGCAGTT AGGTAAAGGA 
AGAAAAAAAC AGATTTACAC CACAGATAGT GATGAGATTT CACATATTGT TAATCGTATT 
GCTCCTCAGG ATGAAAAACC AACAACAAAT TCTATGCTGG ACATGTGGAT TGGTGAAACT 
GCTATTCCTT GTACTCCCAG TGTAGCAAAA GGAAAATCAA GAGCAAAAAT CAGCTGCACA 
AAGTTAAAAA CACAAAGTCA AGAAGAAGAA CTTATGAAAC TGGCTAAACA ATTTGATAAA 
AATATGGAAG AGCTAGATGT GATTCAAGAG CAAAACAAGA GGAATTATGA TTTTACCCAG 
ATGATTTCAG AAACAGAGAT TTTAAGTAAT TATAAAGATA ATATACAGAT GTGGTCATTA 
CATAATATAG TTCCCGAAAT AGATAATGCT ACAAAAAAGC CAATCAAAGG AAACACCAAG 
ATATCTGTGG CAAATAATCA AAATAGCAGT CAGAAGCCAT TTGACCAAAT TGCTGAAGCA 
GCCTTTAATG CTATTTTTGA TGGTTCTACT CAGAAATGTA GCGGACAGTT AAGCCAAGAA 
CTGCCAGAGG CTTTTTGGAG CACCAGTAAT ACTACCTTTG TAAAGACAAA TGCTTTGAAA 
GAGGAGAAAA TCATTACTAA TGAAACTCTG GTCATTGAAA AACTGTCAAA TAAAACCCCA 
CGATCACTTT CTTCTCAAGT AGATACACCC ATAATGACAA AATCATGTGT GACTTCCTGT 
ACTAAGGAGC CAGAAACTTC TAATAAGTAC ATTGATGCAT TTACTACAAG TGATTTTGAG 
GATGATTGGG AAAACTTACT AGGTAGTGAA CCTTTTGCTA TGCAAAATAT CGACATGCCT 
GAACTCTTTC CTTCTAAAAC AGCCCATGTT ACTGATCAAA AGGAAATTTG TACCTTTAAT 
AGTAAAACTG TTAAAAATAC GTCAAGAGCA AATACAAGTC CAGATGCCAG GTTAGGAGAT 
TCAAAAGTAT TACAAGATCT TTCTTCAAAG ACATATGACA GAGAATTAAT AGATGCAGAA 
TATAGATTTT CACCAAATTC AAATAAATCA AACAAATTAT CCACTGGAAA TAAAATGAAA 
TTTGAGAACT CTTCCAATAA AATTGTTATT CAAGACGAAA TTCAAAATTG TATAGTTACA 
TCTAATCTGA CAAAAATAAA GGAAGATATT CTTACTAACT CTACTGAAGC TTCTGAAAGG 
AAGTCAGCTT TGAACACAAG GTATTCTAAT GAACAGAAAA ATAAGTGCAT TTTAAATCAG 
TCTATTAAAG CCCCTGTTAA TACTGATCTT TTTGGCTCTG CAAATCTAGG CAGTAAAACC 
AGTGTTAGTA ACCCAAATCA GACTAGTGCA TCAAAAGTAG GTTCTTTCTT TGATGATTGG 
AATGATCCCT CATTTGCCAA TGAAATTATT AAAGCATGTC ATCAATTAGA TAATACCTGG 
GAAGCAGATG ATGTAGATGA TGATTTGTTG TACCAAGCAT GTGATGATAT TGAAAGACTA 
ACTCAGCAAC AAGACATTAG AAAGGACAGT AAGACATCAG AAAGTATATG TGAGATCAAT 
AATAATTCCG AACATGGAGC CAAAAACATG TTTGCTATAT CTAAACAAGG AAGTAATTTG 
GTACAATCAA AGCATTTGAA TCCAGGCAGC ATTTCAGTGC AGACATCTTT GACAAATAGC 
TCACAAATAG ATAAGCCAAT GAAGATGGAG AAAGGGGAAA TGTATGGAAA TTCTCCAAGA 
TTTTTAGGTG CCACAAATTT GACTATGTAT TCTAAGATCT CAAACTGTCA GATAAATAAT 
CTGCATGTGT CTTATACTAA CACTGATGTT CCAATACAAG TGAATAGTTC CAAATTGGTT 
CTTCCAGGAA GTTCAAGTTT GAATGTAACT TCAGATCATA TGAATACAGA AATTACTACT 
TATAAGAAGA AATTGAGTAC TAATCAGCCA TGCCATAAGA CTGTAACAGA TGAAGCTCAG 
AGCAACCTTA ACACAACAGT TGGATTTTCA AAGTTTACAT TTACAAGGAT GAAAAATTCT 
CAGATTCTTT CTCAGTTTAA TCAAAATTGT ATAACTGGAA GTATGTCTGA TACCAAAATT 
ACACAGGGTG TGGAGAAAAA GAAAGGTGTC AACCCATTAC TGGAGGAAGC TGTTGGACAG 
CAATCTTTGG TGAAACTTTC TGAATCTTTG AAACAATCTT CAAAAGAGGA AGAAGAGAAA 
AATAGAAAGT GTTCTCCTGA AGAAATTCAG AGAAAAAGAC AAGAAGCACT GGTTCGGAGA 
ATGGCTAAAG CACGAGCCTC ATCTGTAAAT GCAGCTCCCA CTTCATTTCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ETAA1 ( XM_017004376.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859865.1 Homo sapiens ETAA1 activator of ATR kinase (ETAA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu89592
Clone ID Related Accession (Same CDS sequence)	XM_017004377.1 , XM_017004376.1
Accession Version	XM_017004376.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ewing's tumor-associated antigen 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCGGC GAAGGAAACA TGATGACAGC CCTAGCCCGA AGAAAACGCC GCACAAAACA 
GTGGCGGCGG AGGAATGCGG CTCGGTGGTC GAGCCAGGGA GGAGGCGGCT GAGATCGGCC 
CGCGGTTCGT GGCCCTGCGG GGCTAGAGAG GGGCCTCCCG GGCCAGTGCG GCAGCGAGAG 
CAGCCTCCGA CCGCCGCCCT GTGCAGTAAA AGTAACCCCG AGGAAAGGTA TGAAACACCA 
AAGAGAGCGC TGAAAATGGA CTCACTGTCA TCTTCCTTCA GTTCTCCTAA TGATCCAGAT 
GGACAGAATG ATATCTTTTG GGATCAGAAT TCTCCATTGA CAAAGCAGTT AGGTAAAGGA 
AGAAAAAAAC AGATTTACAC CACAGATAGT GATGAGATTT CACATATTGT TAATCGTATT 
GCTCCTCAGG ATGAAAAACC AACAACAAAT TCTATGCTGG ACATGTGGAT TGGTGAAACT 
GCTATTCCTT GTACTCCCAG TGTAGCAAAA GGAAAATCAA GAGCAAAAAT CAGCTGCACA 
AAGTTAAAAA CACAAAGTCA AGAAGAAGAA CTTATGAAAC TGGCTAAACA ATTTGATAAA 
AATATGGAAG AGCTAGATGT GATTCAAGAG CAAAACAAGA GGAATTATGA TTTTACCCAG 
ATGATTTCAG AAACAGAGAT TTTAAGTAAT TATAAAGATA ATATACAGAT GTGGTCATTA 
CATAATATAG TTCCCGAAAT AGATAATGCT ACAAAAAAGC CAATCAAAGG AAACACCAAG 
ATATCTGTGG CAAATAATCA AAATAGCAGT CAGAAGCCAT TTGACCAAAT TGCTGAAGCA 
GCCTTTAATG CTATTTTTGA TGGTTCTACT CAGAAATGTA GCGGACAGTT AAGCCAAGAA 
CTGCCAGAGG CTTTTTGGAG CACCAGTAAT ACTACCTTTG TAAAGACAAA TGCTTTGAAA 
GAGGAGAAAA TCATTACTAA TGAAACTCTG GTCATTGAAA AACTGTCAAA TAAAACCCCA 
CGATCACTTT CTTCTCAAGT AGATACACCC ATAATGACAA AATCATGTGT GACTTCCTGT 
ACTAAGGAGC CAGAAACTTC TAATAAGTAC ATTGATGCAT TTACTACAAG TGATTTTGAG 
GATGATTGGG AAAACTTACT AGGTAGTGAA CCTTTTGCTA TGCAAAATAT CGACATGCCT 
GAACTCTTTC CTTCTAAAAC AGCCCATGTT ACTGATCAAA AGGAAATTTG TACCTTTAAT 
AGTAAAACTG TTAAAAATAC GTCAAGAGCA AATACAAGTC CAGATGCCAG GTTAGGAGAT 
TCAAAAGTAT TACAAGATCT TTCTTCAAAG ACATATGACA GAGAATTAAT AGATGCAGAA 
TATAGATTTT CACCAAATTC AAATAAATCA AACAAATTAT CCACTGGAAA TAAAATGAAA 
TTTGAGAACT CTTCCAATAA AATTGTTATT CAAGACGAAA TTCAAAATTG TATAGTTACA 
TCTAATCTGA CAAAAATAAA GGAAGATATT CTTACTAACT CTACTGAAGC TTCTGAAAGG 
AAGTCAGCTT TGAACACAAG GTATTCTAAT GAACAGAAAA ATAAGTGCAT TTTAAATCAG 
TCTATTAAAG CCCCTGTTAA TACTGATCTT TTTGGCTCTG CAAATCTAGG CAGTAAAACC 
AGTGTTAGTA ACCCAAATCA GACTAGTGCA TCAAAAGTAG GTTCTTTCTT TGATGATTGG 
AATGATCCCT CATTTGCCAA TGAAATTATT AAAGCATGTC ATCAATTAGA TAATACCTGG 
GAAGCAGATG ATGTAGATGA TGATTTGTTG TACCAAGCAT GTGATGATAT TGAAAGACTA 
ACTCAGCAAC AAGACATTAG AAAGGACAGT AAGACATCAG AAAGTATATG TGAGATCAAT 
AATAATTCCG AACATGGAGC CAAAAACATG TTTGCTATAT CTAAACAAGG AAGTAATTTG 
GTACAATCAA AGCATTTGAA TCCAGGCAGC ATTTCAGTGC AGACATCTTT GACAAATAGC 
TCACAAATAG ATAAGCCAAT GAAGATGGAG AAAGGGGAAA TGTATGGAAA TTCTCCAAGA 
TTTTTAGGTG CCACAAATTT GACTATGTAT TCTAAGATCT CAAACTGTCA GATAAATAAT 
CTGCATGTGT CTTATACTAA CACTGATGTT CCAATACAAG TGAATAGTTC CAAATTGGTT 
CTTCCAGGAA GTTCAAGTTT GAATGTAACT TCAGATCATA TGAATACAGA AATTACTACT 
TATAAGAAGA AATTGAGTAC TAATCAGCCA TGCCATAAGA CTGTAACAGA TGAAGCTCAG 
AGCAACCTTA ACACAACAGT TGGATTTTCA AAGTTTACAT TTACAAGGAT GAAAAATTCT 
CAGATTCTTT CTCAGTTTAA TCAAAATTGT ATAACTGGAA GTATGTCTGA TACCAAAATT 
ACACAGGGTG TGGAGAAAAA GAAAGGTGTC AACCCATTAC TGGAGGAAGC TGTTGGACAG 
CAATCTTTGG TGAAACTTTC TGAATCTTTG AAACAATCTT CAAAAGAGGA AGAAGAGAAA 
AATAGAAAGT GTTCTCCTGA AGAAATTCAG AGAAAAAGAC AAGAAGCACT GGTTCGGAGA 
ATGGCTAAAG CACGAGCCTC ATCTGTAAAT GCAGCTCCCA CTTCATTTCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859865.1
CDS	133..2826
Translation	MSRRRKHDDSPSPKKTPHKTVAAEECGSVVEPGRRRLRSARGSWPCGAREGPPGPVRQREQPPTAALCSKSNPEERYETPKRALKMDSLSSSFSSPNDPDGQNDIFWDQNSPLTKQLGKGRKKQIYTTDSDEISHIVNRIAPQDEKPTTNSMLDMWIGETAIPCTPSVAKGKSRAKISCTKLKTQSQEEELMKLAKQFDKNMEELDVIQEQNKRNYDFTQMISETEILSNYKDNIQMWSLHNIVPEIDNATKKPIKGNTKISVANNQNSSQKPFDQIAEAAFNAIFDGSTQKCSGQLSQELPEAFWSTSNTTFVKTNALKEEKIITNETLVIEKLSNKTPRSLSSQVDTPIMTKSCVTSCTKEPETSNKYIDAFTTSDFEDDWENLLGSEPFAMQNIDMPELFPSKTAHVTDQKEICTFNSKTVKNTSRANTSPDARLGDSKVLQDLSSKTYDRELIDAEYRFSPNSNKSNKLSTGNKMKFENSSNKIVIQDEIQNCIVTSNLTKIKEDILTNSTEASERKSALNTRYSNEQKNKCILNQSIKAPVNTDLFGSANLGSKTSVSNPNQTSASKVGSFFDDWNDPSFANEIIKACHQLDNTWEADDVDDDLLYQACDDIERLTQQQDIRKDSKTSESICEINNNSEHGAKNMFAISKQGSNLVQSKHLNPGSISVQTSLTNSSQIDKPMKMEKGEMYGNSPRFLGATNLTMYSKISNCQINNLHVSYTNTDVPIQVNSSKLVLPGSSSLNVTSDHMNTEITTYKKKLSTNQPCHKTVTDEAQSNLNTTVGFSKFTFTRMKNSQILSQFNQNCITGSMSDTKITQGVEKKKGVNPLLEEAVGQQSLVKLSESLKQSSKEEEEKNRKCSPEEIQRKRQEALVRRMAKARASSVNAAPTSFL

Target ORF information:

RefSeq Version	XM_017004376.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ETAA1 activator of ATR kinase (ETAA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004376.1

ORF Insert Sequence:

ATGAGTCGGC GAAGGAAACA TGATGACAGC CCTAGCCCGA AGAAAACGCC GCACAAAACA 
GTGGCGGCGG AGGAATGCGG CTCGGTGGTC GAGCCAGGGA GGAGGCGGCT GAGATCGGCC 
CGCGGTTCGT GGCCCTGCGG GGCTAGAGAG GGGCCTCCCG GGCCAGTGCG GCAGCGAGAG 
CAGCCTCCGA CCGCCGCCCT GTGCAGTAAA AGTAACCCCG AGGAAAGGTA TGAAACACCA 
AAGAGAGCGC TGAAAATGGA CTCACTGTCA TCTTCCTTCA GTTCTCCTAA TGATCCAGAT 
GGACAGAATG ATATCTTTTG GGATCAGAAT TCTCCATTGA CAAAGCAGTT AGGTAAAGGA 
AGAAAAAAAC AGATTTACAC CACAGATAGT GATGAGATTT CACATATTGT TAATCGTATT 
GCTCCTCAGG ATGAAAAACC AACAACAAAT TCTATGCTGG ACATGTGGAT TGGTGAAACT 
GCTATTCCTT GTACTCCCAG TGTAGCAAAA GGAAAATCAA GAGCAAAAAT CAGCTGCACA 
AAGTTAAAAA CACAAAGTCA AGAAGAAGAA CTTATGAAAC TGGCTAAACA ATTTGATAAA 
AATATGGAAG AGCTAGATGT GATTCAAGAG CAAAACAAGA GGAATTATGA TTTTACCCAG 
ATGATTTCAG AAACAGAGAT TTTAAGTAAT TATAAAGATA ATATACAGAT GTGGTCATTA 
CATAATATAG TTCCCGAAAT AGATAATGCT ACAAAAAAGC CAATCAAAGG AAACACCAAG 
ATATCTGTGG CAAATAATCA AAATAGCAGT CAGAAGCCAT TTGACCAAAT TGCTGAAGCA 
GCCTTTAATG CTATTTTTGA TGGTTCTACT CAGAAATGTA GCGGACAGTT AAGCCAAGAA 
CTGCCAGAGG CTTTTTGGAG CACCAGTAAT ACTACCTTTG TAAAGACAAA TGCTTTGAAA 
GAGGAGAAAA TCATTACTAA TGAAACTCTG GTCATTGAAA AACTGTCAAA TAAAACCCCA 
CGATCACTTT CTTCTCAAGT AGATACACCC ATAATGACAA AATCATGTGT GACTTCCTGT 
ACTAAGGAGC CAGAAACTTC TAATAAGTAC ATTGATGCAT TTACTACAAG TGATTTTGAG 
GATGATTGGG AAAACTTACT AGGTAGTGAA CCTTTTGCTA TGCAAAATAT CGACATGCCT 
GAACTCTTTC CTTCTAAAAC AGCCCATGTT ACTGATCAAA AGGAAATTTG TACCTTTAAT 
AGTAAAACTG TTAAAAATAC GTCAAGAGCA AATACAAGTC CAGATGCCAG GTTAGGAGAT 
TCAAAAGTAT TACAAGATCT TTCTTCAAAG ACATATGACA GAGAATTAAT AGATGCAGAA 
TATAGATTTT CACCAAATTC AAATAAATCA AACAAATTAT CCACTGGAAA TAAAATGAAA 
TTTGAGAACT CTTCCAATAA AATTGTTATT CAAGACGAAA TTCAAAATTG TATAGTTACA 
TCTAATCTGA CAAAAATAAA GGAAGATATT CTTACTAACT CTACTGAAGC TTCTGAAAGG 
AAGTCAGCTT TGAACACAAG GTATTCTAAT GAACAGAAAA ATAAGTGCAT TTTAAATCAG 
TCTATTAAAG CCCCTGTTAA TACTGATCTT TTTGGCTCTG CAAATCTAGG CAGTAAAACC 
AGTGTTAGTA ACCCAAATCA GACTAGTGCA TCAAAAGTAG GTTCTTTCTT TGATGATTGG 
AATGATCCCT CATTTGCCAA TGAAATTATT AAAGCATGTC ATCAATTAGA TAATACCTGG 
GAAGCAGATG ATGTAGATGA TGATTTGTTG TACCAAGCAT GTGATGATAT TGAAAGACTA 
ACTCAGCAAC AAGACATTAG AAAGGACAGT AAGACATCAG AAAGTATATG TGAGATCAAT 
AATAATTCCG AACATGGAGC CAAAAACATG TTTGCTATAT CTAAACAAGG AAGTAATTTG 
GTACAATCAA AGCATTTGAA TCCAGGCAGC ATTTCAGTGC AGACATCTTT GACAAATAGC 
TCACAAATAG ATAAGCCAAT GAAGATGGAG AAAGGGGAAA TGTATGGAAA TTCTCCAAGA 
TTTTTAGGTG CCACAAATTT GACTATGTAT TCTAAGATCT CAAACTGTCA GATAAATAAT 
CTGCATGTGT CTTATACTAA CACTGATGTT CCAATACAAG TGAATAGTTC CAAATTGGTT 
CTTCCAGGAA GTTCAAGTTT GAATGTAACT TCAGATCATA TGAATACAGA AATTACTACT 
TATAAGAAGA AATTGAGTAC TAATCAGCCA TGCCATAAGA CTGTAACAGA TGAAGCTCAG 
AGCAACCTTA ACACAACAGT TGGATTTTCA AAGTTTACAT TTACAAGGAT GAAAAATTCT 
CAGATTCTTT CTCAGTTTAA TCAAAATTGT ATAACTGGAA GTATGTCTGA TACCAAAATT 
ACACAGGGTG TGGAGAAAAA GAAAGGTGTC AACCCATTAC TGGAGGAAGC TGTTGGACAG 
CAATCTTTGG TGAAACTTTC TGAATCTTTG AAACAATCTT CAAAAGAGGA AGAAGAGAAA 
AATAGAAAGT GTTCTCCTGA AGAAATTCAG AGAAAAAGAC AAGAAGCACT GGTTCGGAGA 
ATGGCTAAAG CACGAGCCTC ATCTGTAAAT GCAGCTCCCA CTTCATTTCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DNA Replication Checkpoint: New ATR Activator Identified.
Current biology : CB27(1)R33-R35(2017 Jan)
Zou L

RPA-Binding Protein ETAA1 Is an ATR Activator Involved in DNA Replication Stress Response.
Current biology : CB26(24)3257-3268(2016 Dec)
Lee YC,Zhou Q,Chen J,Yuan J

ETAA1 acts at stalled replication forks to maintain genome integrity.
Nature cell biology18(11)1185-1195(2016 Nov)
Bass TE,Luzwick JW,Kavanaugh G,Carroll C,Dungrawala H,Glick GG,Feldkamp MD,Putney R,Chazin WJ,Cortez D

Activation of the ATR kinase by the RPA-binding protein ETAA1.
Nature cell biology18(11)1196-1207(2016 Nov)
Haahr P,Hoffmann S,Tollenaere MA,Ho T,Toledo LI,Mann M,Bekker-Jensen S,R?schle M,Mailand N

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ETAA1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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