POLRMT cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLRMT
Entrez Gene ID	5442
Full Name	RNA polymerase mitochondrial
Synonyms	APOLMT,MTRNAP,MTRPOL,h-mtRPOL
General protein information	Preferred Names RNA polymerase mitochondrial Names DNA-directed RNA polymerase, mitochondrial polymerase (RNA) mitochondrial (DNA directed)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.3
Summary	This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes. [provided by RefSeq, Jul 2008].
Disorder MIM:	601778

mRNA and Protein(s)

mRNA	Protein	Name
NM_005035.4	NP_005026.3	DNA-directed RNA polymerase, mitochondrial precursor
XM_005259580.4	XP_005259637.3	DNA-directed RNA polymerase, mitochondrial isoform X1
XM_005259580.3	XP_005259637.2	DNA-directed RNA polymerase, mitochondrial isoform X1
NM_005035.3	NP_005026.3	DNA-directed RNA polymerase, mitochondrial precursor

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	polrmt	XP_004910992.1
Canis lupus familiaris (dog)	POLRMT	XP_005633227.1
Gallus gallus (chicken)	POLRMT	XP_418204.3
Arabidopsis thaliana (thale cress)	SCA3	NP_179989.1
Oryza sativa (rice)	Os06g0652000	NP_001058231.2
Mus musculus (house mouse)	Polrmt	NP_766139.2
Pan troglodytes (chimpanzee)	POLRMT	XP_003316023.1
Bos taurus (cattle)	POLRMT	NP_001192480.1
Danio rerio (zebrafish)	si:dkey-21e2.1	XP_005170578.1
Homo sapiens (human)	POLRMT	NP_005026.3
Rattus norvegicus (Norway rat)	Polrmt	NP_001100236.1
Drosophila melanogaster (fruit fly)	mtRNApol	NP_001259853.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Length heterogeneity at conserved sequence block 2 in human mitochondrial DNA acts as a rheostat for RNA polymerase POLRMT activity.
Tan BG, Wellesley FC, Savery NJ, Szczelkun MD
Nucleic acids research44(16)7817-29(2016 Sep)

Mitochondrial Ribosomal Protein L12 Is Required for POLRMT Stability and Exists as Two Forms Generated by Alternative Proteolysis during Import.
Nouws J, Goswami AV, Bestwick M, McCann BJ, Surovtseva YV, Shadel GS
The Journal of biological chemistry291(2)989-97(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the frequency of variation in sequence identity and length at conserved sequence block 2 amongst human mitochondrial genomes and used in vitro transcription to assess the effects of this length heterogeneity on the activity of the mitochondrial RNA polymerase, POLRMT, was examined.
Title: Length heterogeneity at conserved sequence block 2 in human mitochondrial DNA acts as a rheostat for RNA polymerase POLRMT activity.

knock-down of MRPL12 by RNA interference results in instability of POLRMT.
Title: Mitochondrial Ribosomal Protein L12 Is Required for POLRMT Stability and Exists as Two Forms Generated by Alternative Proteolysis during Import.

Results show that polymorphisms at POLG2 and POLRMT increased risk of oral cancer and leukoplakia, respectively, probably modulating synthesis and activity of the enzymes.
Title: Association of DNA sequence variation in mitochondrial DNA polymerase with mitochondrial DNA synthesis and risk of oral cancer.

Suggest targeting POLRMT as strategy for treating acute myeloid leukemia.
Title: Targeting mitochondrial RNA polymerase in acute myeloid leukemia.

The results reveal the organization of TFAM, POLRMT and TFB2M around the light-strand promoter and represent the first structural characterization of the entire mitochondrial transcriptional initiation complex.
Title: Organization of the human mitochondrial transcription initiation complex.

The following POLRMT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLRMT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu104700	XM_005259580.4 Latest version!	Homo sapiens RNA polymerase mitochondrial (POLRMT), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu19097	NM_005035.4 Latest version!	Homo sapiens RNA polymerase mitochondrial (POLRMT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu59829	XM_005259580.3 Latest version!	Homo sapiens polymerase (RNA) mitochondrial (POLRMT), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
View Old Accession Versions >>
OHu19097	NM_005035.3	Homo sapiens RNA polymerase mitochondrial (POLRMT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLRMT ( XM_005259580.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005259637.3 Homo sapiens RNA polymerase mitochondrial (POLRMT), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu104700
Clone ID Related Accession (Same CDS sequence)	XM_005259580.4
Accession Version	XM_005259580.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005259580.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCAC TTTGCTGGGG CCGCGGAGCG GCGGGGCTCA AACGAGCCCT ACGGCCTTGC 
GGCCGCCCGG GACTCCCCGG CAAAGAAGGG ACCGCCGGTG GCGTCTGCGG CCCCAGGAGG 
AGCTCGTCCG CCAGCCCCCA GGAGCAAGAC CAAGACCGCA GGAAGGACTG GGGCCACGTG 
GAGCTGCTGG AGGTGCTCCA GGCGCGGGTG CGGCAGCTGC AGGCTGAGAG CGTGTCGGAG 
GTGGTGGTGA ACAGGGTGGA TGTGGCGCGG CTCCCAGAAT GTGGCAGTGG AGATGGTAGC 
CTCCAGCCAC CCAGGAAGGT CCAGATGGGG GCCAAGGATG CCACCCCGGT GCCCTGTGGC 
CGCTGGGCAA AGATACTGGA GAAGGATAAG CGGACCCAGC AGATGCGTAT GCAGCGGTTG 
AAGGCGAAGC TGCAGATGCC ATTCCAGAGC GGGGAGTTCA AGGCGCTGAC CAGGCGCCTG 
CAGGTGGAGC CCCGGCTCCT GAGCAAGCAG ATGGCCGGGT GCCTGGAGGA CTGCACGCGC 
CAGGCCCCCG AGAGCCCCTG GGAGGAGCAG CTGGCCCGGC TGCTGCAGGA GGCCCCTGGG 
AAGCTGAGCC TCGATGTGGA GCAGGCCCCG TCGGGGCAGC ACTCGCAGGC CCAGCTCTCA 
GGTCAGCAGC AGAGGCTCCT GGCCTTCTTC AAGTGCTGCC TGCTCACTGA CCAGCTGCCC 
CTCGCCCACC ACCTGCTGGT CGTCCACCAC GGCCAGCGGC AGAAGCGGAA GCTGCTCACG 
CTGGACATGT ACAACGCCGT GATGCTTGGC TGGGCGCGGC AGGGTGCCTT CAAGGAGCTG 
GTATATGTGT TATTCATGGT GAAGGATGCC GGCTTGACTC CGGACCTGCT GTCCTATGCG 
GCTGCCCTCC AGTGCATGGG GAGGCAGGAC CAGGACGCCG GGACCATCGA AAGGTGTCTG 
GAACAGATGA GCCAGGAGGG GCTGAAGCTG CAGGCACTCT TCACCGCCGT TCTGCTGTCT 
GAGGAGGATC GGGCCACTGT TCTGAAGGCC GTGCACAAGG TGAAGCCCAC CTTCAGCCTC 
CCGCCGCAGC TGCCGCCCCC GGTCAACACC TCCAAGCTGC TCAGGGACGT GTATGCCAAG 
GATGGGCGTG TGTCCTACCC GAAGCTGCAC CTGCCCTTGA AGACCCTGCA GTGCCTCTTT 
GAGAAGCAGC TCCACATGGA GCTGGCCAGC AGGGTGTGCG TGGTGTCCGT GGAGAAGCCC 
ACGTTGCCAA GCAAGGAGGT CAAGCACGCG CGGAAGACCC TGAAGACCCT GCGGGACCAA 
TGGGAGAAAG CACTGTGCCG GGCGCTGCGG GAGACCAAGA ACCGCCTAGA GCGCGAGGTG 
TACGAGGGCC GGTTCTCACT TTACCCCTTC CTGTGCCTGC TGGACGAGCG CGAGGTGGTG 
CGGATGCTCC TGCAGGTCCT GCAGGCGCTG CCCGCCCAAG GTGAGTCCTT CACCACCCTG 
GCCCGGGAGC TGAGTGCGCG CACTTTCAGC CGGCACGTGG TGCAGAGGCA GCGGGTCAGT 
GGCCAGGTGC AGGCGCTGCA GAACCACTAC AGGAAGTACC TCTGCTTGCT GGCCTCCGAC 
GCCGAGGTGC CCGAGCCCTG CCTGCCGCGG CAGTACTGGG AGGAGCTGGG GGCGCCCGAG 
GCCCTGCGGG AGCAGCCCTG GCCCCTGCCA GTGCAGATGG AGCTGGGCAA GCTGCTGGCG 
GAGATGCTGG TGCAGGCTAC GCAGATGCCA TGCAGCCTGG ACAAGCCGCA TCGTTCCTCT 
CGGCTTGTCC CCGTGCTCTA CCACGTGTAT TCCTTCCGCA ACGTCCAGCA GATCGGCATC 
CTGAAGCCGC ACCCGGCCTA CGTGCAGCTG CTGGAGAAGG CCGCGGAGCC CACGCTGACC 
TTCGAGGCGG TGGATGTACC CATGCTTTGC CCCCCGCTGC CCTGGACATC GCCGCACTCT 
GGTGCTTTCC TGCTCAGCCC CACCAAGCTG ATGCGCACGG TGGAAGGCGC CACGCAGCAC 
CAGGAGCTGC TGGAAACCTG CCCGCCCACC GCGCTGCATG GCGCACTGGA CGCCCTCACC 
CAACTGGGCA ACTGCGCCTG GCGCGTCAAC GGGCGCGTGC TGGACCTGGT GCTGCAGCTC 
TTCCAGGCCA AGGGCTGCCC CCAGCTAGGC GTGCCGGCCC CGCCCTCCGA GGCGCCCCAG 
CCGCCCGAGG CCCACCTGCC GCACAGCGCC GCGCCCGCCC GCAAGGCCGA GCTGCGCCGT 
GAGCTGGCGC ACTGCCAGAA GGTGGCCCGG GAGATGCACA GCCTGCGGGC GGAGGCGCTG 
TACCGCCTCT CGCTGGCGCA GCACCTGCGG GACCGCGTCT TCTGGCTGCC GCACAACATG 
GACTTCCGCG GCCGCACCTA CCCCTGCCCG CCGCACTTCA ACCACCTGGG CAGCGACGTG 
GCGCGGGCCC TGCTGGAGTT CGCCCAGGGC CGCCCGCTCG GCCCGCACGG CCTGGATTGG 
CTCAAGATCC ACCTGGTCAA TCTCACGGGG TTGAAGAAGC GGGAGCCGCT GCGGAAGCGC 
CTGGCCTTTG CGGAGGAGGT GATGGATGAC ATCCTGGACT CCGCGGACCA ACCCTTGACG 
GGCCGAAAGT GGTGGATGGG CGCGGAGGAA CCCTGGCAGA CGCTGGCCTG CTGTATGGAG 
GTGGCGAACG CTGTGCGCGC CTCCGACCCT GCCGCCTATG TCTCCCACCT CCCCGTCCAT 
CAGGACGGCT CTTGCAACGG CCTGCAGCAT TATGCTGCTC TGGGCCGCGA CAGCGTGGGC 
GCCGCCTCCG TCAACCTGGA GCCCTCGGAT GTGCCGCAGG ACGTGTACAG CGGCGTGGCC 
GCGCAGGTGG AGGTGTTCCG TAGGCAGGAC GCCCAGCGGG GCATGCGGGT GGCACAGGTG 
CTGGAAGGTT TCATCACCCG CAAGGTGGTG AAGCAGACGG TGATGACGGT GGTGTACGGG 
GTCACGCGCT ATGGCGGGCG CCTGCAGATT GAGAAGCGCC TCCGGGAGCT GAGCGACTTT 
CCCCAGGAGT TCGTGTGGGA GGCCTCTCAC TATCTCGTAC GCCAGGTCTT CAAGAGTCTA 
CAGGAGATGT TCTCGGGGAC CCGGGCCATC CAGCACTGGC TGACCGAGAG TGCCCGCCTC 
ATCTCCCACA TGGGCTCTGT GGTGGAGTGG GTCACACCCC TGGGCGTCCC CGTCATCCAG 
CCCTATCGCC TGGACTCCAA GGTCAAGCAA ATAGGAGGTG GAATTCAGAG CATCACCTAC 
ACCCACAACG GAGACATCAG CCGAAAGCCC AACACACGTA AGCAGAAGAA CGGCTTCCCG 
CCCAACTTCA TCCACTCGCT GGACTCCTCC CACATGATGC TCACCGCCCT GCACTGCTAC 
AGGAGTCCTC CGCTCTGCCA CAGGAAGGGC CTGACCTTCG TCTCTGTGCA CGACTGTTAC 
TGGACTCACG CAGCTGATGT CTCCGTCATG AACCAGGTGT GCCGGGAGCA GTTTGTCCGC 
TTGCACAGCG AGCCCATCCT GCAGGACCTG TCCAGATTCC TGGTCAAGCG GTTCTGCTCT 
GAGCCCCAGA AGATCTTGGA GGCCAGCCAG CTGAAGGAGA CACTGCAGGC GGTGCCCAAG 
CCAGGGGCCT TCGACCTGGA GCAGGTGAAG CGTTCCACCT ACTTCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005259637.3
CDS	2..3715
Translation	MSALCWGRGAAGLKRALRPCGRPGLPGKEGTAGGVCGPRRSSSASPQEQDQDRRKDWGHVELLEVLQARVRQLQAESVSEVVVNRVDVARLPECGSGDGSLQPPRKVQMGAKDATPVPCGRWAKILEKDKRTQQMRMQRLKAKLQMPFQSGEFKALTRRLQVEPRLLSKQMAGCLEDCTRQAPESPWEEQLARLLQEAPGKLSLDVEQAPSGQHSQAQLSGQQQRLLAFFKCCLLTDQLPLAHHLLVVHHGQRQKRKLLTLDMYNAVMLGWARQGAFKELVYVLFMVKDAGLTPDLLSYAAALQCMGRQDQDAGTIERCLEQMSQEGLKLQALFTAVLLSEEDRATVLKAVHKVKPTFSLPPQLPPPVNTSKLLRDVYAKDGRVSYPKLHLPLKTLQCLFEKQLHMELASRVCVVSVEKPTLPSKEVKHARKTLKTLRDQWEKALCRALRETKNRLEREVYEGRFSLYPFLCLLDEREVVRMLLQVLQALPAQGESFTTLARELSARTFSRHVVQRQRVSGQVQALQNHYRKYLCLLASDAEVPEPCLPRQYWEELGAPEALREQPWPLPVQMELGKLLAEMLVQATQMPCSLDKPHRSSRLVPVLYHVYSFRNVQQIGILKPHPAYVQLLEKAAEPTLTFEAVDVPMLCPPLPWTSPHSGAFLLSPTKLMRTVEGATQHQELLETCPPTALHGALDALTQLGNCAWRVNGRVLDLVLQLFQAKGCPQLGVPAPPSEAPQPPEAHLPHSAAPARKAELRRELAHCQKVAREMHSLRAEALYRLSLAQHLRDRVFWLPHNMDFRGRTYPCPPHFNHLGSDVARALLEFAQGRPLGPHGLDWLKIHLVNLTGLKKREPLRKRLAFAEEVMDDILDSADQPLTGRKWWMGAEEPWQTLACCMEVANAVRASDPAAYVSHLPVHQDGSCNGLQHYAALGRDSVGAASVNLEPSDVPQDVYSGVAAQVEVFRRQDAQRGMRVAQVLEGFITRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELSDFPQEFVWEASHYLVRQVFKSLQEMFSGTRAIQHWLTESARLISHMGSVVEWVTPLGVPVIQPYRLDSKVKQIGGGIQSITYTHNGDISRKPNTRKQKNGFPPNFIHSLDSSHMMLTALHCYRSPPLCHRKGLTFVSVHDCYWTHAADVSVMNQVCREQFVRLHSEPILQDLSRFLVKRFCSEPQKILEASQLKETLQAVPKPGAFDLEQVKRSTYFFS

Target ORF information:

RefSeq Version	XM_005259580.4
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA polymerase mitochondrial (POLRMT), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005259580.4

ORF Insert Sequence:

ATGTCGGCAC TTTGCTGGGG CCGCGGAGCG GCGGGGCTCA AACGAGCCCT ACGGCCTTGC 
GGCCGCCCGG GACTCCCCGG CAAAGAAGGG ACCGCCGGTG GCGTCTGCGG CCCCAGGAGG 
AGCTCGTCCG CCAGCCCCCA GGAGCAAGAC CAAGACCGCA GGAAGGACTG GGGCCACGTG 
GAGCTGCTGG AGGTGCTCCA GGCGCGGGTG CGGCAGCTGC AGGCTGAGAG CGTGTCGGAG 
GTGGTGGTGA ACAGGGTGGA TGTGGCGCGG CTCCCAGAAT GTGGCAGTGG AGATGGTAGC 
CTCCAGCCAC CCAGGAAGGT CCAGATGGGG GCCAAGGATG CCACCCCGGT GCCCTGTGGC 
CGCTGGGCAA AGATACTGGA GAAGGATAAG CGGACCCAGC AGATGCGTAT GCAGCGGTTG 
AAGGCGAAGC TGCAGATGCC ATTCCAGAGC GGGGAGTTCA AGGCGCTGAC CAGGCGCCTG 
CAGGTGGAGC CCCGGCTCCT GAGCAAGCAG ATGGCCGGGT GCCTGGAGGA CTGCACGCGC 
CAGGCCCCCG AGAGCCCCTG GGAGGAGCAG CTGGCCCGGC TGCTGCAGGA GGCCCCTGGG 
AAGCTGAGCC TCGATGTGGA GCAGGCCCCG TCGGGGCAGC ACTCGCAGGC CCAGCTCTCA 
GGTCAGCAGC AGAGGCTCCT GGCCTTCTTC AAGTGCTGCC TGCTCACTGA CCAGCTGCCC 
CTCGCCCACC ACCTGCTGGT CGTCCACCAC GGCCAGCGGC AGAAGCGGAA GCTGCTCACG 
CTGGACATGT ACAACGCCGT GATGCTTGGC TGGGCGCGGC AGGGTGCCTT CAAGGAGCTG 
GTATATGTGT TATTCATGGT GAAGGATGCC GGCTTGACTC CGGACCTGCT GTCCTATGCG 
GCTGCCCTCC AGTGCATGGG GAGGCAGGAC CAGGACGCCG GGACCATCGA AAGGTGTCTG 
GAACAGATGA GCCAGGAGGG GCTGAAGCTG CAGGCACTCT TCACCGCCGT TCTGCTGTCT 
GAGGAGGATC GGGCCACTGT TCTGAAGGCC GTGCACAAGG TGAAGCCCAC CTTCAGCCTC 
CCGCCGCAGC TGCCGCCCCC GGTCAACACC TCCAAGCTGC TCAGGGACGT GTATGCCAAG 
GATGGGCGTG TGTCCTACCC GAAGCTGCAC CTGCCCTTGA AGACCCTGCA GTGCCTCTTT 
GAGAAGCAGC TCCACATGGA GCTGGCCAGC AGGGTGTGCG TGGTGTCCGT GGAGAAGCCC 
ACGTTGCCAA GCAAGGAGGT CAAGCACGCG CGGAAGACCC TGAAGACCCT GCGGGACCAA 
TGGGAGAAAG CACTGTGCCG GGCGCTGCGG GAGACCAAGA ACCGCCTAGA GCGCGAGGTG 
TACGAGGGCC GGTTCTCACT TTACCCCTTC CTGTGCCTGC TGGACGAGCG CGAGGTGGTG 
CGGATGCTCC TGCAGGTCCT GCAGGCGCTG CCCGCCCAAG GTGAGTCCTT CACCACCCTG 
GCCCGGGAGC TGAGTGCGCG CACTTTCAGC CGGCACGTGG TGCAGAGGCA GCGGGTCAGT 
GGCCAGGTGC AGGCGCTGCA GAACCACTAC AGGAAGTACC TCTGCTTGCT GGCCTCCGAC 
GCCGAGGTGC CCGAGCCCTG CCTGCCGCGG CAGTACTGGG AGGAGCTGGG GGCGCCCGAG 
GCCCTGCGGG AGCAGCCCTG GCCCCTGCCA GTGCAGATGG AGCTGGGCAA GCTGCTGGCG 
GAGATGCTGG TGCAGGCTAC GCAGATGCCA TGCAGCCTGG ACAAGCCGCA TCGTTCCTCT 
CGGCTTGTCC CCGTGCTCTA CCACGTGTAT TCCTTCCGCA ACGTCCAGCA GATCGGCATC 
CTGAAGCCGC ACCCGGCCTA CGTGCAGCTG CTGGAGAAGG CCGCGGAGCC CACGCTGACC 
TTCGAGGCGG TGGATGTACC CATGCTTTGC CCCCCGCTGC CCTGGACATC GCCGCACTCT 
GGTGCTTTCC TGCTCAGCCC CACCAAGCTG ATGCGCACGG TGGAAGGCGC CACGCAGCAC 
CAGGAGCTGC TGGAAACCTG CCCGCCCACC GCGCTGCATG GCGCACTGGA CGCCCTCACC 
CAACTGGGCA ACTGCGCCTG GCGCGTCAAC GGGCGCGTGC TGGACCTGGT GCTGCAGCTC 
TTCCAGGCCA AGGGCTGCCC CCAGCTAGGC GTGCCGGCCC CGCCCTCCGA GGCGCCCCAG 
CCGCCCGAGG CCCACCTGCC GCACAGCGCC GCGCCCGCCC GCAAGGCCGA GCTGCGCCGT 
GAGCTGGCGC ACTGCCAGAA GGTGGCCCGG GAGATGCACA GCCTGCGGGC GGAGGCGCTG 
TACCGCCTCT CGCTGGCGCA GCACCTGCGG GACCGCGTCT TCTGGCTGCC GCACAACATG 
GACTTCCGCG GCCGCACCTA CCCCTGCCCG CCGCACTTCA ACCACCTGGG CAGCGACGTG 
GCGCGGGCCC TGCTGGAGTT CGCCCAGGGC CGCCCGCTCG GCCCGCACGG CCTGGATTGG 
CTCAAGATCC ACCTGGTCAA TCTCACGGGG TTGAAGAAGC GGGAGCCGCT GCGGAAGCGC 
CTGGCCTTTG CGGAGGAGGT GATGGATGAC ATCCTGGACT CCGCGGACCA ACCCTTGACG 
GGCCGAAAGT GGTGGATGGG CGCGGAGGAA CCCTGGCAGA CGCTGGCCTG CTGTATGGAG 
GTGGCGAACG CTGTGCGCGC CTCCGACCCT GCCGCCTATG TCTCCCACCT CCCCGTCCAT 
CAGGACGGCT CTTGCAACGG CCTGCAGCAT TATGCTGCTC TGGGCCGCGA CAGCGTGGGC 
GCCGCCTCCG TCAACCTGGA GCCCTCGGAT GTGCCGCAGG ACGTGTACAG CGGCGTGGCC 
GCGCAGGTGG AGGTGTTCCG TAGGCAGGAC GCCCAGCGGG GCATGCGGGT GGCACAGGTG 
CTGGAAGGTT TCATCACCCG CAAGGTGGTG AAGCAGACGG TGATGACGGT GGTGTACGGG 
GTCACGCGCT ATGGCGGGCG CCTGCAGATT GAGAAGCGCC TCCGGGAGCT GAGCGACTTT 
CCCCAGGAGT TCGTGTGGGA GGCCTCTCAC TATCTCGTAC GCCAGGTCTT CAAGAGTCTA 
CAGGAGATGT TCTCGGGGAC CCGGGCCATC CAGCACTGGC TGACCGAGAG TGCCCGCCTC 
ATCTCCCACA TGGGCTCTGT GGTGGAGTGG GTCACACCCC TGGGCGTCCC CGTCATCCAG 
CCCTATCGCC TGGACTCCAA GGTCAAGCAA ATAGGAGGTG GAATTCAGAG CATCACCTAC 
ACCCACAACG GAGACATCAG CCGAAAGCCC AACACACGTA AGCAGAAGAA CGGCTTCCCG 
CCCAACTTCA TCCACTCGCT GGACTCCTCC CACATGATGC TCACCGCCCT GCACTGCTAC 
AGGAGTCCTC CGCTCTGCCA CAGGAAGGGC CTGACCTTCG TCTCTGTGCA CGACTGTTAC 
TGGACTCACG CAGCTGATGT CTCCGTCATG AACCAGGTGT GCCGGGAGCA GTTTGTCCGC 
TTGCACAGCG AGCCCATCCT GCAGGACCTG TCCAGATTCC TGGTCAAGCG GTTCTGCTCT 
GAGCCCCAGA AGATCTTGGA GGCCAGCCAG CTGAAGGAGA CACTGCAGGC GGTGCCCAAG 
CCAGGGGCCT TCGACCTGGA GCAGGTGAAG CGTTCCACCT ACTTCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLRMT ( NM_005035.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005026.3 Homo sapiens RNA polymerase mitochondrial (POLRMT), mRNA.

Price & Availability↑

CloneID	OHu19097
Clone ID Related Accession (Same CDS sequence)	NM_005035.4 , NM_005035.3
Accession Version	NM_005035.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC070057.1, BC030829.1, BC098387.1 and AC004449.1. This sequence is a reference standard in the RefSeqGene project. On Nov 24, 2018 this sequence version replaced NM_005035.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC098387.1, U75370.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000588649.7/ ENSP00000465759.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCGGCAC TTTGCTGGGG CCGCGGAGCG GCGGGGCTCA AACGAGCCCT ACGGCCTTGC 
GGCCGCCCGG GACTCCCCGG CAAAGAAGGG ACCGCCGGTG GCGTCTGCGG CCCCAGGAGG 
AGCTCGTCCG CCAGCCCCCA GGAGCAAGAC CAAGACCGCA GGAAGGACTG GGGCCACGTG 
GAGCTGCTGG AGGTGCTCCA GGCGCGGGTG CGGCAGCTGC AGGCTGAGAG CGTGTCGGAG 
GTGGTGGTGA ACAGGGTGGA TGTGGCGCGG CTCCCAGAAT GTGGCAGTGG AGATGGTAGC 
CTCCAGCCAC CCAGGAAGGT CCAGATGGGG GCCAAGGATG CCACCCCGGT GCCCTGTGGC 
CGCTGGGCAA AGATACTGGA GAAGGATAAG CGGACCCAGC AGATGCGTAT GCAGCGGTTG 
AAGGCGAAGC TGCAGATGCC ATTCCAGAGC GGGGAGTTCA AGGCGCTGAC CAGGCGCCTG 
CAGGTGGAGC CCCGGCTCCT GAGCAAGCAG ATGGCCGGGT GCCTGGAGGA CTGCACGCGC 
CAGGCCCCCG AGAGCCCCTG GGAGGAGCAG CTGGCCCGGC TGCTGCAGGA GGCCCCTGGG 
AAGCTGAGCC TCGATGTGGA GCAGGCCCCG TCGGGGCAGC ACTCGCAGGC CCAGCTCTCA 
GGTCAGCAGC AGAGGCTCCT GGCCTTCTTC AAGTGCTGCC TGCTCACTGA CCAGCTGCCC 
CTCGCCCACC ACCTGCTGGT CGTCCACCAC GGCCAGCGGC AGAAGCGGAA GCTGCTCACG 
CTGGACATGT ACAACGCCGT GATGCTTGGC TGGGCGCGGC AGGGTGCCTT CAAGGAGCTG 
GTATATGTGT TATTCATGGT GAAGGATGCC GGCTTGACTC CGGACCTGCT GTCCTATGCG 
GCTGCCCTCC AGTGCATGGG GAGGCAGGAC CAGGACGCCG GGACCATCGA AAGGTGTCTG 
GAACAGATGA GCCAGGAGGG GCTGAAGCTG CAGGCACTCT TCACCGCCGT TCTGCTGTCT 
GAGGAGGATC GGGCCACTGT TCTGAAGGCC GTGCACAAGG TGAAGCCCAC CTTCAGCCTC 
CCGCCGCAGC TGCCGCCCCC GGTCAACACC TCCAAGCTGC TCAGGGACGT GTATGCCAAG 
GATGGGCGTG TGTCCTACCC GAAGCTGCAC CTGCCCTTGA AGACCCTGCA GTGCCTCTTT 
GAGAAGCAGC TCCACATGGA GCTGGCCAGC AGGGTGTGCG TGGTGTCCGT GGAGAAGCCC 
ACGTTGCCAA GCAAGGAGGT CAAGCACGCG CGGAAGACCC TGAAGACCCT GCGGGACCAA 
TGGGAGAAAG CACTGTGCCG GGCGCTGCGG GAGACCAAGA ACCGCCTAGA GCGCGAGGTG 
TACGAGGGCC GGTTCTCACT TTACCCCTTC CTGTGCCTGC TGGACGAGCG CGAGGTGGTG 
CGGATGCTCC TGCAGGTCCT GCAGGCGCTG CCCGCCCAAG GTGAGTCCTT CACCACCCTG 
GCCCGGGAGC TGAGTGCGCG CACTTTCAGC CGGCACGTGG TGCAGAGGCA GCGGGTCAGT 
GGCCAGGTGC AGGCGCTGCA GAACCACTAC AGGAAGTACC TCTGCTTGCT GGCCTCCGAC 
GCCGAGGTGC CCGAGCCCTG CCTGCCGCGG CAGTACTGGG AGGAGCTGGG GGCGCCCGAG 
GCCCTGCGGG AGCAGCCCTG GCCCCTGCCA GTGCAGATGG AGCTGGGCAA GCTGCTGGCG 
GAGATGCTGG TGCAGGCTAC GCAGATGCCA TGCAGCCTGG ACAAGCCGCA TCGTTCCTCT 
CGGCTTGTCC CCGTGCTCTA CCACGTGTAT TCCTTCCGCA ACGTCCAGCA GATCGGCATC 
CTGAAGCCGC ACCCGGCCTA CGTGCAGCTG CTGGAGAAGG CCGCGGAGCC CACGCTGACC 
TTCGAGGCGG TGGATGTACC CATGCTTTGC CCCCCGCTGC CCTGGACATC GCCGCACTCT 
GGTGCTTTCC TGCTCAGCCC CACCAAGCTG ATGCGCACGG TGGAAGGCGC CACGCAGCAC 
CAGGAGCTGC TGGAAACCTG CCCGCCCACC GCGCTGCATG GCGCACTGGA CGCCCTCACC 
CAACTGGGCA ACTGCGCCTG GCGCGTCAAC GGGCGCGTGC TGGACCTGGT GCTGCAGCTC 
TTCCAGGCCA AGGGCTGCCC CCAGCTAGGC GTGCCGGCCC CGCCCTCCGA GGCGCCCCAG 
CCGCCCGAGG CCCACCTGCC GCACAGCGCC GCGCCCGCCC GCAAGGCCGA GCTGCGCCGT 
GAGCTGGCGC ACTGCCAGAA GGTGGCCCGG GAGATGCACA GCCTGCGGGC GGAGGCGCTG 
TACCGCCTCT CGCTGGCGCA GCACCTGCGG GACCGCGTCT TCTGGCTGCC GCACAACATG 
GACTTCCGCG GCCGCACCTA CCCCTGCCCG CCGCACTTCA ACCACCTGGG CAGCGACGTG 
GCGCGGGCCC TGCTGGAGTT CGCCCAGGGC CGCCCGCTCG GCCCGCACGG CCTGGATTGG 
CTCAAGATCC ACCTGGTCAA TCTCACGGGG TTGAAGAAGC GGGAGCCGCT GCGGAAGCGC 
CTGGCCTTTG CGGAGGAGGT GATGGATGAC ATCCTGGACT CCGCGGACCA ACCCTTGACG 
GGCCGAAAGT GGTGGATGGG CGCGGAGGAA CCCTGGCAGA CGCTGGCCTG CTGTATGGAG 
GTGGCGAACG CTGTGCGCGC CTCCGACCCT GCCGCCTATG TCTCCCACCT CCCCGTCCAT 
CAGGACGGCT CTTGCAACGG CCTGCAGCAT TATGCTGCTC TGGGCCGCGA CAGCGTGGGC 
GCCGCCTCCG TCAACCTGGA GCCCTCGGAT GTGCCGCAGG ACGTGTACAG CGGCGTGGCC 
GCGCAGGTGG AGGTGTTCCG TAGGCAGGAC GCCCAGCGGG GCATGCGGGT GGCACAGGTG 
CTGGAAGGTT TCATCACCCG CAAGGTGGTG AAGCAGACGG TGATGACGGT GGTGTACGGG 
GTCACGCGCT ATGGCGGGCG CCTGCAGATT GAGAAGCGCC TCCGGGAGCT GAGCGACTTT 
CCCCAGGAGT TCGTGTGGGA GGCCTCTCAC TATCTCGTAC GCCAGGTCTT CAAGAGTCTA 
CAGGAGATGT TCTCGGGGAC CCGGGCCATC CAGCACTGGC TGACCGAGAG TGCCCGCCTC 
ATCTCCCACA TGGGCTCTGT GGTGGAGTGG GTCACACCCC TGGGCGTCCC CGTCATCCAG 
CCCTATCGCC TGGACTCCAA GGTCAAGCAA ATAGGAGGTG GAATTCAGAG CATCACCTAC 
ACCCACAACG GAGACATCAG CCGAAAGCCC AACACACGTA AGCAGAAGAA CGGCTTCCCG 
CCCAACTTCA TCCACTCGCT GGACTCCTCC CACATGATGC TCACCGCCCT GCACTGCTAC 
AGGAAGGGCC TGACCTTCGT CTCTGTGCAC GACTGTTACT GGACTCACGC AGCTGATGTC 
TCCGTCATGA ACCAGGTGTG CCGGGAGCAG TTTGTCCGCT TGCACAGCGA GCCCATCCTG 
CAGGACCTGT CCAGATTCCT GGTCAAGCGG TTCTGCTCTG AGCCCCAGAA GATCTTGGAG 
GCCAGCCAGC TGAAGGAGAC ACTGCAGGCG GTGCCCAAGC CAGGGGCCTT CGACCTGGAG 
CAGGTGAAGC GTTCCACCTA CTTCTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005026.3
CDS	26..3718
Translation	MSALCWGRGAAGLKRALRPCGRPGLPGKEGTAGGVCGPRRSSSASPQEQDQDRRKDWGHVELLEVLQARVRQLQAESVSEVVVNRVDVARLPECGSGDGSLQPPRKVQMGAKDATPVPCGRWAKILEKDKRTQQMRMQRLKAKLQMPFQSGEFKALTRRLQVEPRLLSKQMAGCLEDCTRQAPESPWEEQLARLLQEAPGKLSLDVEQAPSGQHSQAQLSGQQQRLLAFFKCCLLTDQLPLAHHLLVVHHGQRQKRKLLTLDMYNAVMLGWARQGAFKELVYVLFMVKDAGLTPDLLSYAAALQCMGRQDQDAGTIERCLEQMSQEGLKLQALFTAVLLSEEDRATVLKAVHKVKPTFSLPPQLPPPVNTSKLLRDVYAKDGRVSYPKLHLPLKTLQCLFEKQLHMELASRVCVVSVEKPTLPSKEVKHARKTLKTLRDQWEKALCRALRETKNRLEREVYEGRFSLYPFLCLLDEREVVRMLLQVLQALPAQGESFTTLARELSARTFSRHVVQRQRVSGQVQALQNHYRKYLCLLASDAEVPEPCLPRQYWEELGAPEALREQPWPLPVQMELGKLLAEMLVQATQMPCSLDKPHRSSRLVPVLYHVYSFRNVQQIGILKPHPAYVQLLEKAAEPTLTFEAVDVPMLCPPLPWTSPHSGAFLLSPTKLMRTVEGATQHQELLETCPPTALHGALDALTQLGNCAWRVNGRVLDLVLQLFQAKGCPQLGVPAPPSEAPQPPEAHLPHSAAPARKAELRRELAHCQKVAREMHSLRAEALYRLSLAQHLRDRVFWLPHNMDFRGRTYPCPPHFNHLGSDVARALLEFAQGRPLGPHGLDWLKIHLVNLTGLKKREPLRKRLAFAEEVMDDILDSADQPLTGRKWWMGAEEPWQTLACCMEVANAVRASDPAAYVSHLPVHQDGSCNGLQHYAALGRDSVGAASVNLEPSDVPQDVYSGVAAQVEVFRRQDAQRGMRVAQVLEGFITRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELSDFPQEFVWEASHYLVRQVFKSLQEMFSGTRAIQHWLTESARLISHMGSVVEWVTPLGVPVIQPYRLDSKVKQIGGGIQSITYTHNGDISRKPNTRKQKNGFPPNFIHSLDSSHMMLTALHCYRKGLTFVSVHDCYWTHAADVSVMNQVCREQFVRLHSEPILQDLSRFLVKRFCSEPQKILEASQLKETLQAVPKPGAFDLEQVKRSTYFFS

Target ORF information:

RefSeq Version	NM_005035.4
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA polymerase mitochondrial (POLRMT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005035.4

ORF Insert Sequence:

ATGTCGGCAC TTTGCTGGGG CCGCGGAGCG GCGGGGCTCA AACGAGCCCT ACGGCCTTGC 
GGCCGCCCGG GACTCCCCGG CAAAGAAGGG ACCGCCGGTG GCGTCTGCGG CCCCAGGAGG 
AGCTCGTCCG CCAGCCCCCA GGAGCAAGAC CAAGACCGCA GGAAGGACTG GGGCCACGTG 
GAGCTGCTGG AGGTGCTCCA GGCGCGGGTG CGGCAGCTGC AGGCTGAGAG CGTGTCGGAG 
GTGGTGGTGA ACAGGGTGGA TGTGGCGCGG CTCCCAGAAT GTGGCAGTGG AGATGGTAGC 
CTCCAGCCAC CCAGGAAGGT CCAGATGGGG GCCAAGGATG CCACCCCGGT GCCCTGTGGC 
CGCTGGGCAA AGATACTGGA GAAGGATAAG CGGACCCAGC AGATGCGTAT GCAGCGGTTG 
AAGGCGAAGC TGCAGATGCC ATTCCAGAGC GGGGAGTTCA AGGCGCTGAC CAGGCGCCTG 
CAGGTGGAGC CCCGGCTCCT GAGCAAGCAG ATGGCCGGGT GCCTGGAGGA CTGCACGCGC 
CAGGCCCCCG AGAGCCCCTG GGAGGAGCAG CTGGCCCGGC TGCTGCAGGA GGCCCCTGGG 
AAGCTGAGCC TCGATGTGGA GCAGGCCCCG TCGGGGCAGC ACTCGCAGGC CCAGCTCTCA 
GGTCAGCAGC AGAGGCTCCT GGCCTTCTTC AAGTGCTGCC TGCTCACTGA CCAGCTGCCC 
CTCGCCCACC ACCTGCTGGT CGTCCACCAC GGCCAGCGGC AGAAGCGGAA GCTGCTCACG 
CTGGACATGT ACAACGCCGT GATGCTTGGC TGGGCGCGGC AGGGTGCCTT CAAGGAGCTG 
GTATATGTGT TATTCATGGT GAAGGATGCC GGCTTGACTC CGGACCTGCT GTCCTATGCG 
GCTGCCCTCC AGTGCATGGG GAGGCAGGAC CAGGACGCCG GGACCATCGA AAGGTGTCTG 
GAACAGATGA GCCAGGAGGG GCTGAAGCTG CAGGCACTCT TCACCGCCGT TCTGCTGTCT 
GAGGAGGATC GGGCCACTGT TCTGAAGGCC GTGCACAAGG TGAAGCCCAC CTTCAGCCTC 
CCGCCGCAGC TGCCGCCCCC GGTCAACACC TCCAAGCTGC TCAGGGACGT GTATGCCAAG 
GATGGGCGTG TGTCCTACCC GAAGCTGCAC CTGCCCTTGA AGACCCTGCA GTGCCTCTTT 
GAGAAGCAGC TCCACATGGA GCTGGCCAGC AGGGTGTGCG TGGTGTCCGT GGAGAAGCCC 
ACGTTGCCAA GCAAGGAGGT CAAGCACGCG CGGAAGACCC TGAAGACCCT GCGGGACCAA 
TGGGAGAAAG CACTGTGCCG GGCGCTGCGG GAGACCAAGA ACCGCCTAGA GCGCGAGGTG 
TACGAGGGCC GGTTCTCACT TTACCCCTTC CTGTGCCTGC TGGACGAGCG CGAGGTGGTG 
CGGATGCTCC TGCAGGTCCT GCAGGCGCTG CCCGCCCAAG GTGAGTCCTT CACCACCCTG 
GCCCGGGAGC TGAGTGCGCG CACTTTCAGC CGGCACGTGG TGCAGAGGCA GCGGGTCAGT 
GGCCAGGTGC AGGCGCTGCA GAACCACTAC AGGAAGTACC TCTGCTTGCT GGCCTCCGAC 
GCCGAGGTGC CCGAGCCCTG CCTGCCGCGG CAGTACTGGG AGGAGCTGGG GGCGCCCGAG 
GCCCTGCGGG AGCAGCCCTG GCCCCTGCCA GTGCAGATGG AGCTGGGCAA GCTGCTGGCG 
GAGATGCTGG TGCAGGCTAC GCAGATGCCA TGCAGCCTGG ACAAGCCGCA TCGTTCCTCT 
CGGCTTGTCC CCGTGCTCTA CCACGTGTAT TCCTTCCGCA ACGTCCAGCA GATCGGCATC 
CTGAAGCCGC ACCCGGCCTA CGTGCAGCTG CTGGAGAAGG CCGCGGAGCC CACGCTGACC 
TTCGAGGCGG TGGATGTACC CATGCTTTGC CCCCCGCTGC CCTGGACATC GCCGCACTCT 
GGTGCTTTCC TGCTCAGCCC CACCAAGCTG ATGCGCACGG TGGAAGGCGC CACGCAGCAC 
CAGGAGCTGC TGGAAACCTG CCCGCCCACC GCGCTGCATG GCGCACTGGA CGCCCTCACC 
CAACTGGGCA ACTGCGCCTG GCGCGTCAAC GGGCGCGTGC TGGACCTGGT GCTGCAGCTC 
TTCCAGGCCA AGGGCTGCCC CCAGCTAGGC GTGCCGGCCC CGCCCTCCGA GGCGCCCCAG 
CCGCCCGAGG CCCACCTGCC GCACAGCGCC GCGCCCGCCC GCAAGGCCGA GCTGCGCCGT 
GAGCTGGCGC ACTGCCAGAA GGTGGCCCGG GAGATGCACA GCCTGCGGGC GGAGGCGCTG 
TACCGCCTCT CGCTGGCGCA GCACCTGCGG GACCGCGTCT TCTGGCTGCC GCACAACATG 
GACTTCCGCG GCCGCACCTA CCCCTGCCCG CCGCACTTCA ACCACCTGGG CAGCGACGTG 
GCGCGGGCCC TGCTGGAGTT CGCCCAGGGC CGCCCGCTCG GCCCGCACGG CCTGGATTGG 
CTCAAGATCC ACCTGGTCAA TCTCACGGGG TTGAAGAAGC GGGAGCCGCT GCGGAAGCGC 
CTGGCCTTTG CGGAGGAGGT GATGGATGAC ATCCTGGACT CCGCGGACCA ACCCTTGACG 
GGCCGAAAGT GGTGGATGGG CGCGGAGGAA CCCTGGCAGA CGCTGGCCTG CTGTATGGAG 
GTGGCGAACG CTGTGCGCGC CTCCGACCCT GCCGCCTATG TCTCCCACCT CCCCGTCCAT 
CAGGACGGCT CTTGCAACGG CCTGCAGCAT TATGCTGCTC TGGGCCGCGA CAGCGTGGGC 
GCCGCCTCCG TCAACCTGGA GCCCTCGGAT GTGCCGCAGG ACGTGTACAG CGGCGTGGCC 
GCGCAGGTGG AGGTGTTCCG TAGGCAGGAC GCCCAGCGGG GCATGCGGGT GGCACAGGTG 
CTGGAAGGTT TCATCACCCG CAAGGTGGTG AAGCAGACGG TGATGACGGT GGTGTACGGG 
GTCACGCGCT ATGGCGGGCG CCTGCAGATT GAGAAGCGCC TCCGGGAGCT GAGCGACTTT 
CCCCAGGAGT TCGTGTGGGA GGCCTCTCAC TATCTCGTAC GCCAGGTCTT CAAGAGTCTA 
CAGGAGATGT TCTCGGGGAC CCGGGCCATC CAGCACTGGC TGACCGAGAG TGCCCGCCTC 
ATCTCCCACA TGGGCTCTGT GGTGGAGTGG GTCACACCCC TGGGCGTCCC CGTCATCCAG 
CCCTATCGCC TGGACTCCAA GGTCAAGCAA ATAGGAGGTG GAATTCAGAG CATCACCTAC 
ACCCACAACG GAGACATCAG CCGAAAGCCC AACACACGTA AGCAGAAGAA CGGCTTCCCG 
CCCAACTTCA TCCACTCGCT GGACTCCTCC CACATGATGC TCACCGCCCT GCACTGCTAC 
AGGAAGGGCC TGACCTTCGT CTCTGTGCAC GACTGTTACT GGACTCACGC AGCTGATGTC 
TCCGTCATGA ACCAGGTGTG CCGGGAGCAG TTTGTCCGCT TGCACAGCGA GCCCATCCTG 
CAGGACCTGT CCAGATTCCT GGTCAAGCGG TTCTGCTCTG AGCCCCAGAA GATCTTGGAG 
GCCAGCCAGC TGAAGGAGAC ACTGCAGGCG GTGCCCAAGC CAGGGGCCTT CGACCTGGAG 
CAGGTGAAGC GTTCCACCTA CTTCTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLRMT ( NM_005035.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005026.3 Homo sapiens RNA polymerase mitochondrial (POLRMT), mRNA.

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CloneID	OHu19097
Clone ID Related Accession (Same CDS sequence)	NM_005035.4 , NM_005035.3
Accession Version	NM_005035.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC070057.1, BC030829.1 and BC098387.1. This sequence is a reference standard in the RefSeqGene project. On Jul 21, 2006 this sequence version replaced NM_005035.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC098387.1, U75370.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal (PMID:9097968) gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCGGCAC TTTGCTGGGG CCGCGGAGCG GCGGGGCTCA AACGAGCCCT ACGGCCTTGC 
GGCCGCCCGG GACTCCCCGG CAAAGAAGGG ACCGCCGGTG GCGTCTGCGG CCCCAGGAGG 
AGCTCGTCCG CCAGCCCCCA GGAGCAAGAC CAAGACCGCA GGAAGGACTG GGGCCACGTG 
GAGCTGCTGG AGGTGCTCCA GGCGCGGGTG CGGCAGCTGC AGGCTGAGAG CGTGTCGGAG 
GTGGTGGTGA ACAGGGTGGA TGTGGCGCGG CTCCCAGAAT GTGGCAGTGG AGATGGTAGC 
CTCCAGCCAC CCAGGAAGGT CCAGATGGGG GCCAAGGATG CCACCCCGGT GCCCTGTGGC 
CGCTGGGCAA AGATACTGGA GAAGGATAAG CGGACCCAGC AGATGCGTAT GCAGCGGTTG 
AAGGCGAAGC TGCAGATGCC ATTCCAGAGC GGGGAGTTCA AGGCGCTGAC CAGGCGCCTG 
CAGGTGGAGC CCCGGCTCCT GAGCAAGCAG ATGGCCGGGT GCCTGGAGGA CTGCACGCGC 
CAGGCCCCCG AGAGCCCCTG GGAGGAGCAG CTGGCCCGGC TGCTGCAGGA GGCCCCTGGG 
AAGCTGAGCC TCGATGTGGA GCAGGCCCCG TCGGGGCAGC ACTCGCAGGC CCAGCTCTCA 
GGTCAGCAGC AGAGGCTCCT GGCCTTCTTC AAGTGCTGCC TGCTCACTGA CCAGCTGCCC 
CTCGCCCACC ACCTGCTGGT CGTCCACCAC GGCCAGCGGC AGAAGCGGAA GCTGCTCACG 
CTGGACATGT ACAACGCCGT GATGCTTGGC TGGGCGCGGC AGGGTGCCTT CAAGGAGCTG 
GTATATGTGT TATTCATGGT GAAGGATGCC GGCTTGACTC CGGACCTGCT GTCCTATGCG 
GCTGCCCTCC AGTGCATGGG GAGGCAGGAC CAGGACGCCG GGACCATCGA AAGGTGTCTG 
GAACAGATGA GCCAGGAGGG GCTGAAGCTG CAGGCACTCT TCACCGCCGT TCTGCTGTCT 
GAGGAGGATC GGGCCACTGT TCTGAAGGCC GTGCACAAGG TGAAGCCCAC CTTCAGCCTC 
CCGCCGCAGC TGCCGCCCCC GGTCAACACC TCCAAGCTGC TCAGGGACGT GTATGCCAAG 
GATGGGCGTG TGTCCTACCC GAAGCTGCAC CTGCCCTTGA AGACCCTGCA GTGCCTCTTT 
GAGAAGCAGC TCCACATGGA GCTGGCCAGC AGGGTGTGCG TGGTGTCCGT GGAGAAGCCC 
ACGTTGCCAA GCAAGGAGGT CAAGCACGCG CGGAAGACCC TGAAGACCCT GCGGGACCAA 
TGGGAGAAAG CACTGTGCCG GGCGCTGCGG GAGACCAAGA ACCGCCTAGA GCGCGAGGTG 
TACGAGGGCC GGTTCTCACT TTACCCCTTC CTGTGCCTGC TGGACGAGCG CGAGGTGGTG 
CGGATGCTCC TGCAGGTCCT GCAGGCGCTG CCCGCCCAAG GTGAGTCCTT CACCACCCTG 
GCCCGGGAGC TGAGTGCGCG CACTTTCAGC CGGCACGTGG TGCAGAGGCA GCGGGTCAGT 
GGCCAGGTGC AGGCGCTGCA GAACCACTAC AGGAAGTACC TCTGCTTGCT GGCCTCCGAC 
GCCGAGGTGC CCGAGCCCTG CCTGCCGCGG CAGTACTGGG AGGAGCTGGG GGCGCCCGAG 
GCCCTGCGGG AGCAGCCCTG GCCCCTGCCA GTGCAGATGG AGCTGGGCAA GCTGCTGGCG 
GAGATGCTGG TGCAGGCTAC GCAGATGCCA TGCAGCCTGG ACAAGCCGCA TCGTTCCTCT 
CGGCTTGTCC CCGTGCTCTA CCACGTGTAT TCCTTCCGCA ACGTCCAGCA GATCGGCATC 
CTGAAGCCGC ACCCGGCCTA CGTGCAGCTG CTGGAGAAGG CCGCGGAGCC CACGCTGACC 
TTCGAGGCGG TGGATGTACC CATGCTTTGC CCCCCGCTGC CCTGGACATC GCCGCACTCT 
GGTGCTTTCC TGCTCAGCCC CACCAAGCTG ATGCGCACGG TGGAAGGCGC CACGCAGCAC 
CAGGAGCTGC TGGAAACCTG CCCGCCCACC GCGCTGCATG GCGCACTGGA CGCCCTCACC 
CAACTGGGCA ACTGCGCCTG GCGCGTCAAC GGGCGCGTGC TGGACCTGGT GCTGCAGCTC 
TTCCAGGCCA AGGGCTGCCC CCAGCTAGGC GTGCCGGCCC CGCCCTCCGA GGCGCCCCAG 
CCGCCCGAGG CCCACCTGCC GCACAGCGCC GCGCCCGCCC GCAAGGCCGA GCTGCGCCGT 
GAGCTGGCGC ACTGCCAGAA GGTGGCCCGG GAGATGCACA GCCTGCGGGC GGAGGCGCTG 
TACCGCCTCT CGCTGGCGCA GCACCTGCGG GACCGCGTCT TCTGGCTGCC GCACAACATG 
GACTTCCGCG GCCGCACCTA CCCCTGCCCG CCGCACTTCA ACCACCTGGG CAGCGACGTG 
GCGCGGGCCC TGCTGGAGTT CGCCCAGGGC CGCCCGCTCG GCCCGCACGG CCTGGATTGG 
CTCAAGATCC ACCTGGTCAA TCTCACGGGG TTGAAGAAGC GGGAGCCGCT GCGGAAGCGC 
CTGGCCTTTG CGGAGGAGGT GATGGATGAC ATCCTGGACT CCGCGGACCA ACCCTTGACG 
GGCCGAAAGT GGTGGATGGG CGCGGAGGAA CCCTGGCAGA CGCTGGCCTG CTGTATGGAG 
GTGGCGAACG CTGTGCGCGC CTCCGACCCT GCCGCCTATG TCTCCCACCT CCCCGTCCAT 
CAGGACGGCT CTTGCAACGG CCTGCAGCAT TATGCTGCTC TGGGCCGCGA CAGCGTGGGC 
GCCGCCTCCG TCAACCTGGA GCCCTCGGAT GTGCCGCAGG ACGTGTACAG CGGCGTGGCC 
GCGCAGGTGG AGGTGTTCCG TAGGCAGGAC GCCCAGCGGG GCATGCGGGT GGCACAGGTG 
CTGGAAGGTT TCATCACCCG CAAGGTGGTG AAGCAGACGG TGATGACGGT GGTGTACGGG 
GTCACGCGCT ATGGCGGGCG CCTGCAGATT GAGAAGCGCC TCCGGGAGCT GAGCGACTTT 
CCCCAGGAGT TCGTGTGGGA GGCCTCTCAC TATCTCGTAC GCCAGGTCTT CAAGAGTCTA 
CAGGAGATGT TCTCGGGGAC CCGGGCCATC CAGCACTGGC TGACCGAGAG TGCCCGCCTC 
ATCTCCCACA TGGGCTCTGT GGTGGAGTGG GTCACACCCC TGGGCGTCCC CGTCATCCAG 
CCCTATCGCC TGGACTCCAA GGTCAAGCAA ATAGGAGGTG GAATTCAGAG CATCACCTAC 
ACCCACAACG GAGACATCAG CCGAAAGCCC AACACACGTA AGCAGAAGAA CGGCTTCCCG 
CCCAACTTCA TCCACTCGCT GGACTCCTCC CACATGATGC TCACCGCCCT GCACTGCTAC 
AGGAAGGGCC TGACCTTCGT CTCTGTGCAC GACTGTTACT GGACTCACGC AGCTGATGTC 
TCCGTCATGA ACCAGGTGTG CCGGGAGCAG TTTGTCCGCT TGCACAGCGA GCCCATCCTG 
CAGGACCTGT CCAGATTCCT GGTCAAGCGG TTCTGCTCTG AGCCCCAGAA GATCTTGGAG 
GCCAGCCAGC TGAAGGAGAC ACTGCAGGCG GTGCCCAAGC CAGGGGCCTT CGACCTGGAG 
CAGGTGAAGC GTTCCACCTA CTTCTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005026.3
CDS	57..3749
Translation	MSALCWGRGAAGLKRALRPCGRPGLPGKEGTAGGVCGPRRSSSASPQEQDQDRRKDWGHVELLEVLQARVRQLQAESVSEVVVNRVDVARLPECGSGDGSLQPPRKVQMGAKDATPVPCGRWAKILEKDKRTQQMRMQRLKAKLQMPFQSGEFKALTRRLQVEPRLLSKQMAGCLEDCTRQAPESPWEEQLARLLQEAPGKLSLDVEQAPSGQHSQAQLSGQQQRLLAFFKCCLLTDQLPLAHHLLVVHHGQRQKRKLLTLDMYNAVMLGWARQGAFKELVYVLFMVKDAGLTPDLLSYAAALQCMGRQDQDAGTIERCLEQMSQEGLKLQALFTAVLLSEEDRATVLKAVHKVKPTFSLPPQLPPPVNTSKLLRDVYAKDGRVSYPKLHLPLKTLQCLFEKQLHMELASRVCVVSVEKPTLPSKEVKHARKTLKTLRDQWEKALCRALRETKNRLEREVYEGRFSLYPFLCLLDEREVVRMLLQVLQALPAQGESFTTLARELSARTFSRHVVQRQRVSGQVQALQNHYRKYLCLLASDAEVPEPCLPRQYWEELGAPEALREQPWPLPVQMELGKLLAEMLVQATQMPCSLDKPHRSSRLVPVLYHVYSFRNVQQIGILKPHPAYVQLLEKAAEPTLTFEAVDVPMLCPPLPWTSPHSGAFLLSPTKLMRTVEGATQHQELLETCPPTALHGALDALTQLGNCAWRVNGRVLDLVLQLFQAKGCPQLGVPAPPSEAPQPPEAHLPHSAAPARKAELRRELAHCQKVAREMHSLRAEALYRLSLAQHLRDRVFWLPHNMDFRGRTYPCPPHFNHLGSDVARALLEFAQGRPLGPHGLDWLKIHLVNLTGLKKREPLRKRLAFAEEVMDDILDSADQPLTGRKWWMGAEEPWQTLACCMEVANAVRASDPAAYVSHLPVHQDGSCNGLQHYAALGRDSVGAASVNLEPSDVPQDVYSGVAAQVEVFRRQDAQRGMRVAQVLEGFITRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELSDFPQEFVWEASHYLVRQVFKSLQEMFSGTRAIQHWLTESARLISHMGSVVEWVTPLGVPVIQPYRLDSKVKQIGGGIQSITYTHNGDISRKPNTRKQKNGFPPNFIHSLDSSHMMLTALHCYRKGLTFVSVHDCYWTHAADVSVMNQVCREQFVRLHSEPILQDLSRFLVKRFCSEPQKILEASQLKETLQAVPKPGAFDLEQVKRSTYFFS

Target ORF information:

RefSeq Version	NM_005035.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA polymerase mitochondrial (POLRMT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005035.3

ORF Insert Sequence:

ATGTCGGCAC TTTGCTGGGG CCGCGGAGCG GCGGGGCTCA AACGAGCCCT ACGGCCTTGC 
GGCCGCCCGG GACTCCCCGG CAAAGAAGGG ACCGCCGGTG GCGTCTGCGG CCCCAGGAGG 
AGCTCGTCCG CCAGCCCCCA GGAGCAAGAC CAAGACCGCA GGAAGGACTG GGGCCACGTG 
GAGCTGCTGG AGGTGCTCCA GGCGCGGGTG CGGCAGCTGC AGGCTGAGAG CGTGTCGGAG 
GTGGTGGTGA ACAGGGTGGA TGTGGCGCGG CTCCCAGAAT GTGGCAGTGG AGATGGTAGC 
CTCCAGCCAC CCAGGAAGGT CCAGATGGGG GCCAAGGATG CCACCCCGGT GCCCTGTGGC 
CGCTGGGCAA AGATACTGGA GAAGGATAAG CGGACCCAGC AGATGCGTAT GCAGCGGTTG 
AAGGCGAAGC TGCAGATGCC ATTCCAGAGC GGGGAGTTCA AGGCGCTGAC CAGGCGCCTG 
CAGGTGGAGC CCCGGCTCCT GAGCAAGCAG ATGGCCGGGT GCCTGGAGGA CTGCACGCGC 
CAGGCCCCCG AGAGCCCCTG GGAGGAGCAG CTGGCCCGGC TGCTGCAGGA GGCCCCTGGG 
AAGCTGAGCC TCGATGTGGA GCAGGCCCCG TCGGGGCAGC ACTCGCAGGC CCAGCTCTCA 
GGTCAGCAGC AGAGGCTCCT GGCCTTCTTC AAGTGCTGCC TGCTCACTGA CCAGCTGCCC 
CTCGCCCACC ACCTGCTGGT CGTCCACCAC GGCCAGCGGC AGAAGCGGAA GCTGCTCACG 
CTGGACATGT ACAACGCCGT GATGCTTGGC TGGGCGCGGC AGGGTGCCTT CAAGGAGCTG 
GTATATGTGT TATTCATGGT GAAGGATGCC GGCTTGACTC CGGACCTGCT GTCCTATGCG 
GCTGCCCTCC AGTGCATGGG GAGGCAGGAC CAGGACGCCG GGACCATCGA AAGGTGTCTG 
GAACAGATGA GCCAGGAGGG GCTGAAGCTG CAGGCACTCT TCACCGCCGT TCTGCTGTCT 
GAGGAGGATC GGGCCACTGT TCTGAAGGCC GTGCACAAGG TGAAGCCCAC CTTCAGCCTC 
CCGCCGCAGC TGCCGCCCCC GGTCAACACC TCCAAGCTGC TCAGGGACGT GTATGCCAAG 
GATGGGCGTG TGTCCTACCC GAAGCTGCAC CTGCCCTTGA AGACCCTGCA GTGCCTCTTT 
GAGAAGCAGC TCCACATGGA GCTGGCCAGC AGGGTGTGCG TGGTGTCCGT GGAGAAGCCC 
ACGTTGCCAA GCAAGGAGGT CAAGCACGCG CGGAAGACCC TGAAGACCCT GCGGGACCAA 
TGGGAGAAAG CACTGTGCCG GGCGCTGCGG GAGACCAAGA ACCGCCTAGA GCGCGAGGTG 
TACGAGGGCC GGTTCTCACT TTACCCCTTC CTGTGCCTGC TGGACGAGCG CGAGGTGGTG 
CGGATGCTCC TGCAGGTCCT GCAGGCGCTG CCCGCCCAAG GTGAGTCCTT CACCACCCTG 
GCCCGGGAGC TGAGTGCGCG CACTTTCAGC CGGCACGTGG TGCAGAGGCA GCGGGTCAGT 
GGCCAGGTGC AGGCGCTGCA GAACCACTAC AGGAAGTACC TCTGCTTGCT GGCCTCCGAC 
GCCGAGGTGC CCGAGCCCTG CCTGCCGCGG CAGTACTGGG AGGAGCTGGG GGCGCCCGAG 
GCCCTGCGGG AGCAGCCCTG GCCCCTGCCA GTGCAGATGG AGCTGGGCAA GCTGCTGGCG 
GAGATGCTGG TGCAGGCTAC GCAGATGCCA TGCAGCCTGG ACAAGCCGCA TCGTTCCTCT 
CGGCTTGTCC CCGTGCTCTA CCACGTGTAT TCCTTCCGCA ACGTCCAGCA GATCGGCATC 
CTGAAGCCGC ACCCGGCCTA CGTGCAGCTG CTGGAGAAGG CCGCGGAGCC CACGCTGACC 
TTCGAGGCGG TGGATGTACC CATGCTTTGC CCCCCGCTGC CCTGGACATC GCCGCACTCT 
GGTGCTTTCC TGCTCAGCCC CACCAAGCTG ATGCGCACGG TGGAAGGCGC CACGCAGCAC 
CAGGAGCTGC TGGAAACCTG CCCGCCCACC GCGCTGCATG GCGCACTGGA CGCCCTCACC 
CAACTGGGCA ACTGCGCCTG GCGCGTCAAC GGGCGCGTGC TGGACCTGGT GCTGCAGCTC 
TTCCAGGCCA AGGGCTGCCC CCAGCTAGGC GTGCCGGCCC CGCCCTCCGA GGCGCCCCAG 
CCGCCCGAGG CCCACCTGCC GCACAGCGCC GCGCCCGCCC GCAAGGCCGA GCTGCGCCGT 
GAGCTGGCGC ACTGCCAGAA GGTGGCCCGG GAGATGCACA GCCTGCGGGC GGAGGCGCTG 
TACCGCCTCT CGCTGGCGCA GCACCTGCGG GACCGCGTCT TCTGGCTGCC GCACAACATG 
GACTTCCGCG GCCGCACCTA CCCCTGCCCG CCGCACTTCA ACCACCTGGG CAGCGACGTG 
GCGCGGGCCC TGCTGGAGTT CGCCCAGGGC CGCCCGCTCG GCCCGCACGG CCTGGATTGG 
CTCAAGATCC ACCTGGTCAA TCTCACGGGG TTGAAGAAGC GGGAGCCGCT GCGGAAGCGC 
CTGGCCTTTG CGGAGGAGGT GATGGATGAC ATCCTGGACT CCGCGGACCA ACCCTTGACG 
GGCCGAAAGT GGTGGATGGG CGCGGAGGAA CCCTGGCAGA CGCTGGCCTG CTGTATGGAG 
GTGGCGAACG CTGTGCGCGC CTCCGACCCT GCCGCCTATG TCTCCCACCT CCCCGTCCAT 
CAGGACGGCT CTTGCAACGG CCTGCAGCAT TATGCTGCTC TGGGCCGCGA CAGCGTGGGC 
GCCGCCTCCG TCAACCTGGA GCCCTCGGAT GTGCCGCAGG ACGTGTACAG CGGCGTGGCC 
GCGCAGGTGG AGGTGTTCCG TAGGCAGGAC GCCCAGCGGG GCATGCGGGT GGCACAGGTG 
CTGGAAGGTT TCATCACCCG CAAGGTGGTG AAGCAGACGG TGATGACGGT GGTGTACGGG 
GTCACGCGCT ATGGCGGGCG CCTGCAGATT GAGAAGCGCC TCCGGGAGCT GAGCGACTTT 
CCCCAGGAGT TCGTGTGGGA GGCCTCTCAC TATCTCGTAC GCCAGGTCTT CAAGAGTCTA 
CAGGAGATGT TCTCGGGGAC CCGGGCCATC CAGCACTGGC TGACCGAGAG TGCCCGCCTC 
ATCTCCCACA TGGGCTCTGT GGTGGAGTGG GTCACACCCC TGGGCGTCCC CGTCATCCAG 
CCCTATCGCC TGGACTCCAA GGTCAAGCAA ATAGGAGGTG GAATTCAGAG CATCACCTAC 
ACCCACAACG GAGACATCAG CCGAAAGCCC AACACACGTA AGCAGAAGAA CGGCTTCCCG 
CCCAACTTCA TCCACTCGCT GGACTCCTCC CACATGATGC TCACCGCCCT GCACTGCTAC 
AGGAAGGGCC TGACCTTCGT CTCTGTGCAC GACTGTTACT GGACTCACGC AGCTGATGTC 
TCCGTCATGA ACCAGGTGTG CCGGGAGCAG TTTGTCCGCT TGCACAGCGA GCCCATCCTG 
CAGGACCTGT CCAGATTCCT GGTCAAGCGG TTCTGCTCTG AGCCCCAGAA GATCTTGGAG 
GCCAGCCAGC TGAAGGAGAC ACTGCAGGCG GTGCCCAAGC CAGGGGCCTT CGACCTGGAG 
CAGGTGAAGC GTTCCACCTA CTTCTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLRMT ( XM_005259580.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005259637.2 Homo sapiens polymerase (RNA) mitochondrial (POLRMT), transcript variant X2, mRNA.

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CloneID	OHu59829
Clone ID Related Accession (Same CDS sequence)	XM_005259580.3
Accession Version	XM_005259580.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005259580.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGGC GCGGGCCGGT GGGGTGGCCT GGAGCGGCGT GCGTAATGTC GGCACTTTGC 
TGGGGCCGCG GAGCGGCGGG GCTCAAACGA GCCCTACGGC CTTGCGGCCG CCCGGGACTC 
CCCGGCAAAG AAGGGACCGC CGGTGGCGTC TGCGGCCCCA GGAGGAGCTC GTCCGCCAGC 
CCCCAGGAGC AAGACCAAGA CCGCAGGAAG GACTGGGGCC ACGTGGAGCT GCTGGAGGTG 
CTCCAGGCGC GGGTGCGGCA GCTGCAGGCT GAGAGCGTGT CGGAGGTGGT GGTGAACAGG 
GTGGATGTGG CGCGGCTCCC AGAATGTGGC AGTGGAGATG GTAGCCTCCA GCCACCCAGG 
AAGGTCCAGA TGGGGGCCAA GGATGCCACC CCGGTGCCCT GTGGCCGCTG GGCAAAGATA 
CTGGAGAAGG ATAAGCGGAC CCAGCAGATG CGTATGCAGC GGTTGAAGGC GAAGCTGCAG 
ATGCCATTCC AGAGCGGGGA GTTCAAGGCG CTGACCAGGC GCCTGCAGGT GGAGCCCCGG 
CTCCTGAGCA AGCAGATGGC CGGGTGCCTG GAGGACTGCA CGCGCCAGGC CCCCGAGAGC 
CCCTGGGAGG AGCAGCTGGC CCGGCTGCTG CAGGAGGCCC CTGGGAAGCT GAGCCTCGAT 
GTGGAGCAGG CCCCGTCGGG GCAGCACTCG CAGGCCCAGC TCTCAGGTCA GCAGCAGAGG 
CTCCTGGCCT TCTTCAAGTG CTGCCTGCTC ACTGACCAGC TGCCCCTCGC CCACCACCTG 
CTGGTCGTCC ACCACGGCCA GCGGCAGAAG CGGAAGCTGC TCACGCTGGA CATGTACAAC 
GCCGTGATGC TTGGCTGGGC GCGGCAGGGT GCCTTCAAGG AGCTGGTATA TGTGTTATTC 
ATGGTGAAGG ATGCCGGCTT GACTCCGGAC CTGCTGTCCT ATGCGGCTGC CCTCCAGTGC 
ATGGGGAGGC AGGACCAGGA CGCCGGGACC ATCGAAAGGT GTCTGGAACA GATGAGCCAG 
GAGGGGCTGA AGCTGCAGGC ACTCTTCACC GCCGTTCTGC TGTCTGAGGA GGATCGGGCC 
ACTGTTCTGA AGGCCGTGCA CAAGGTGAAG CCCACCTTCA GCCTCCCGCC GCAGCTGCCG 
CCCCCGGTCA ACACCTCCAA GCTGCTCAGG GACGTGTATG CCAAGGATGG GCGTGTGTCC 
TACCCGAAGC TGCACCTGCC CTTGAAGACC CTGCAGTGCC TCTTTGAGAA GCAGCTCCAC 
ATGGAGCTGG CCAGCAGGGT GTGCGTGGTG TCCGTGGAGA AGCCCACGTT GCCAAGCAAG 
GAGGTCAAGC ACGCGCGGAA GACCCTGAAG ACCCTGCGGG ACCAATGGGA GAAAGCACTG 
TGCCGGGCGC TGCGGGAGAC CAAGAACCGC CTAGAGCGCG AGGTGTACGA GGGCCGGTTC 
TCACTTTACC CCTTCCTGTG CCTGCTGGAC GAGCGCGAGG TGGTGCGGAT GCTCCTGCAG 
GTCCTGCAGG CGCTGCCCGC CCAAGGTGAG TCCTTCACCA CCCTGGCCCG GGAGCTGAGT 
GCGCGCACTT TCAGCCGGCA CGTGGTGCAG AGGCAGCGGG TCAGTGGCCA GGTGCAGGCG 
CTGCAGAACC ACTACAGGAA GTACCTCTGC TTGCTGGCCT CCGACGCCGA GGTGCCCGAG 
CCCTGCCTGC CGCGGCAGTA CTGGGAGGAG CTGGGGGCGC CCGAGGCCCT GCGGGAGCAG 
CCCTGGCCCC TGCCAGTGCA GATGGAGCTG GGCAAGCTGC TGGCGGAGAT GCTGGTGCAG 
GCTACGCAGA TGCCATGCAG CCTGGACAAG CCGCATCGTT CCTCTCGGCT TGTCCCCGTG 
CTCTACCACG TGTATTCCTT CCGCAACGTC CAGCAGATCG GCATCCTGAA GCCGCACCCG 
GCCTACGTGC AGCTGCTGGA GAAGGCCGCG GAGCCCACGC TGACCTTCGA GGCGGTGGAT 
GTACCCATGC TTTGCCCCCC GCTGCCCTGG ACATCGCCGC ACTCTGGTGC TTTCCTGCTC 
AGCCCCACCA AGCTGATGCG CACGGTGGAA GGCGCCACGC AGCACCAGGA GCTGCTGGAA 
ACCTGCCCGC CCACCGCGCT GCATGGCGCA CTGGACGCCC TCACCCAACT GGGCAACTGC 
GCCTGGCGCG TCAACGGGCG CGTGCTGGAC CTGGTGCTGC AGCTCTTCCA GGCCAAGGGC 
TGCCCCCAGC TAGGCGTGCC GGCCCCGCCC TCCGAGGCGC CCCAGCCGCC CGAGGCCCAC 
CTGCCGCACA GCGCCGCGCC CGCCCGCAAG GCCGAGCTGC GCCGTGAGCT GGCGCACTGC 
CAGAAGGTGG CCCGGGAGAT GCACAGCCTG CGGGCGGAGG CGCTGTACCG CCTCTCGCTG 
GCGCAGCACC TGCGGGACCG CGTCTTCTGG CTGCCGCACA ACATGGACTT CCGCGGCCGC 
ACCTACCCCT GCCCGCCGCA CTTCAACCAC CTGGGCAGCG ACGTGGCGCG GGCCCTGCTG 
GAGTTCGCCC AGGGCCGCCC GCTCGGCCCG CACGGCCTGG ATTGGCTCAA GATCCACCTG 
GTCAATCTCA CGGGGTTGAA GAAGCGGGAG CCGCTGCGGA AGCGCCTGGC CTTTGCGGAG 
GAGGTGATGG ATGACATCCT GGACTCCGCG GACCAACCCT TGACGGGCCG AAAGTGGTGG 
ATGGGCGCGG AGGAACCCTG GCAGACGCTG GCCTGCTGTA TGGAGGTGGC GAACGCTGTG 
CGCGCCTCCG ACCCTGCCGC CTATGTCTCC CACCTCCCCG TCCATCAGGA CGGCTCTTGC 
AACGGCCTGC AGCATTATGC TGCTCTGGGC CGCGACAGCG TGGGCGCCGC CTCCGTCAAC 
CTGGAGCCCT CGGATGTGCC GCAGGACGTG TACAGCGGCG TGGCCGCGCA GGTGGAGGTG 
TTCCGTAGGC AGGACGCCCA GCGGGGCATG CGGGTGGCAC AGGTGCTGGA AGGTTTCATC 
ACCCGCAAGG TGGTGAAGCA GACGGTGATG ACGGTGGTGT ACGGGGTCAC GCGCTATGGC 
GGGCGCCTGC AGATTGAGAA GCGCCTCCGG GAGCTGAGCG ACTTTCCCCA GGAGTTCGTG 
TGGGAGGCCT CTCACTATCT CGTACGCCAG GTCTTCAAGA GTCTACAGGA GATGTTCTCG 
GGGACCCGGG CCATCCAGCA CTGGCTGACC GAGAGTGCCC GCCTCATCTC CCACATGGGC 
TCTGTGGTGG AGTGGGTCAC ACCCCTGGGC GTCCCCGTCA TCCAGCCCTA TCGCCTGGAC 
TCCAAGGTCA AGCAAATAGG AGGTGGAATT CAGAGCATCA CCTACACCCA CAACGGAGAC 
ATCAGCCGAA AGCCCAACAC ACGTAAGCAG AAGAACGGCT TCCCGCCCAA CTTCATCCAC 
TCGCTGGACT CCTCCCACAT GATGCTCACC GCCCTGCACT GCTACAGGAG TCCTCCGCTC 
TGCCACAGGA AGGGCCTGAC CTTCGTCTCT GTGCACGACT GTTACTGGAC TCACGCAGCT 
GATGTCTCCG TCATGAACCA GGTGTGCCGG GAGCAGTTTG TCCGCTTGCA CAGCGAGCCC 
ATCCTGCAGG ACCTGTCCAG ATTCCTGGTC AAGCGGTTCT GCTCTGAGCC CCAGAAGATC 
TTGGAGGCCA GCCAGCTGAA GGAGACACTG CAGGCGGTGC CCAAGCCAGG GGCCTTCGAC 
CTGGAGCAGG TGAAGCGTTC CACCTACTTC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005259637.2
CDS	36..3794
Translation	MRRRGPVGWPGAACVMSALCWGRGAAGLKRALRPCGRPGLPGKEGTAGGVCGPRRSSSASPQEQDQDRRKDWGHVELLEVLQARVRQLQAESVSEVVVNRVDVARLPECGSGDGSLQPPRKVQMGAKDATPVPCGRWAKILEKDKRTQQMRMQRLKAKLQMPFQSGEFKALTRRLQVEPRLLSKQMAGCLEDCTRQAPESPWEEQLARLLQEAPGKLSLDVEQAPSGQHSQAQLSGQQQRLLAFFKCCLLTDQLPLAHHLLVVHHGQRQKRKLLTLDMYNAVMLGWARQGAFKELVYVLFMVKDAGLTPDLLSYAAALQCMGRQDQDAGTIERCLEQMSQEGLKLQALFTAVLLSEEDRATVLKAVHKVKPTFSLPPQLPPPVNTSKLLRDVYAKDGRVSYPKLHLPLKTLQCLFEKQLHMELASRVCVVSVEKPTLPSKEVKHARKTLKTLRDQWEKALCRALRETKNRLEREVYEGRFSLYPFLCLLDEREVVRMLLQVLQALPAQGESFTTLARELSARTFSRHVVQRQRVSGQVQALQNHYRKYLCLLASDAEVPEPCLPRQYWEELGAPEALREQPWPLPVQMELGKLLAEMLVQATQMPCSLDKPHRSSRLVPVLYHVYSFRNVQQIGILKPHPAYVQLLEKAAEPTLTFEAVDVPMLCPPLPWTSPHSGAFLLSPTKLMRTVEGATQHQELLETCPPTALHGALDALTQLGNCAWRVNGRVLDLVLQLFQAKGCPQLGVPAPPSEAPQPPEAHLPHSAAPARKAELRRELAHCQKVAREMHSLRAEALYRLSLAQHLRDRVFWLPHNMDFRGRTYPCPPHFNHLGSDVARALLEFAQGRPLGPHGLDWLKIHLVNLTGLKKREPLRKRLAFAEEVMDDILDSADQPLTGRKWWMGAEEPWQTLACCMEVANAVRASDPAAYVSHLPVHQDGSCNGLQHYAALGRDSVGAASVNLEPSDVPQDVYSGVAAQVEVFRRQDAQRGMRVAQVLEGFITRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELSDFPQEFVWEASHYLVRQVFKSLQEMFSGTRAIQHWLTESARLISHMGSVVEWVTPLGVPVIQPYRLDSKVKQIGGGIQSITYTHNGDISRKPNTRKQKNGFPPNFIHSLDSSHMMLTALHCYRSPPLCHRKGLTFVSVHDCYWTHAADVSVMNQVCREQFVRLHSEPILQDLSRFLVKRFCSEPQKILEASQLKETLQAVPKPGAFDLEQVKRSTYFFS

Target ORF information:

RefSeq Version	XM_005259580.3
Organism	Homo sapiens(human)
Definition	Homo sapiens polymerase (RNA) mitochondrial (POLRMT), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005259580.3

ORF Insert Sequence:

ATGCGCAGGC GCGGGCCGGT GGGGTGGCCT GGAGCGGCGT GCGTAATGTC GGCACTTTGC 
TGGGGCCGCG GAGCGGCGGG GCTCAAACGA GCCCTACGGC CTTGCGGCCG CCCGGGACTC 
CCCGGCAAAG AAGGGACCGC CGGTGGCGTC TGCGGCCCCA GGAGGAGCTC GTCCGCCAGC 
CCCCAGGAGC AAGACCAAGA CCGCAGGAAG GACTGGGGCC ACGTGGAGCT GCTGGAGGTG 
CTCCAGGCGC GGGTGCGGCA GCTGCAGGCT GAGAGCGTGT CGGAGGTGGT GGTGAACAGG 
GTGGATGTGG CGCGGCTCCC AGAATGTGGC AGTGGAGATG GTAGCCTCCA GCCACCCAGG 
AAGGTCCAGA TGGGGGCCAA GGATGCCACC CCGGTGCCCT GTGGCCGCTG GGCAAAGATA 
CTGGAGAAGG ATAAGCGGAC CCAGCAGATG CGTATGCAGC GGTTGAAGGC GAAGCTGCAG 
ATGCCATTCC AGAGCGGGGA GTTCAAGGCG CTGACCAGGC GCCTGCAGGT GGAGCCCCGG 
CTCCTGAGCA AGCAGATGGC CGGGTGCCTG GAGGACTGCA CGCGCCAGGC CCCCGAGAGC 
CCCTGGGAGG AGCAGCTGGC CCGGCTGCTG CAGGAGGCCC CTGGGAAGCT GAGCCTCGAT 
GTGGAGCAGG CCCCGTCGGG GCAGCACTCG CAGGCCCAGC TCTCAGGTCA GCAGCAGAGG 
CTCCTGGCCT TCTTCAAGTG CTGCCTGCTC ACTGACCAGC TGCCCCTCGC CCACCACCTG 
CTGGTCGTCC ACCACGGCCA GCGGCAGAAG CGGAAGCTGC TCACGCTGGA CATGTACAAC 
GCCGTGATGC TTGGCTGGGC GCGGCAGGGT GCCTTCAAGG AGCTGGTATA TGTGTTATTC 
ATGGTGAAGG ATGCCGGCTT GACTCCGGAC CTGCTGTCCT ATGCGGCTGC CCTCCAGTGC 
ATGGGGAGGC AGGACCAGGA CGCCGGGACC ATCGAAAGGT GTCTGGAACA GATGAGCCAG 
GAGGGGCTGA AGCTGCAGGC ACTCTTCACC GCCGTTCTGC TGTCTGAGGA GGATCGGGCC 
ACTGTTCTGA AGGCCGTGCA CAAGGTGAAG CCCACCTTCA GCCTCCCGCC GCAGCTGCCG 
CCCCCGGTCA ACACCTCCAA GCTGCTCAGG GACGTGTATG CCAAGGATGG GCGTGTGTCC 
TACCCGAAGC TGCACCTGCC CTTGAAGACC CTGCAGTGCC TCTTTGAGAA GCAGCTCCAC 
ATGGAGCTGG CCAGCAGGGT GTGCGTGGTG TCCGTGGAGA AGCCCACGTT GCCAAGCAAG 
GAGGTCAAGC ACGCGCGGAA GACCCTGAAG ACCCTGCGGG ACCAATGGGA GAAAGCACTG 
TGCCGGGCGC TGCGGGAGAC CAAGAACCGC CTAGAGCGCG AGGTGTACGA GGGCCGGTTC 
TCACTTTACC CCTTCCTGTG CCTGCTGGAC GAGCGCGAGG TGGTGCGGAT GCTCCTGCAG 
GTCCTGCAGG CGCTGCCCGC CCAAGGTGAG TCCTTCACCA CCCTGGCCCG GGAGCTGAGT 
GCGCGCACTT TCAGCCGGCA CGTGGTGCAG AGGCAGCGGG TCAGTGGCCA GGTGCAGGCG 
CTGCAGAACC ACTACAGGAA GTACCTCTGC TTGCTGGCCT CCGACGCCGA GGTGCCCGAG 
CCCTGCCTGC CGCGGCAGTA CTGGGAGGAG CTGGGGGCGC CCGAGGCCCT GCGGGAGCAG 
CCCTGGCCCC TGCCAGTGCA GATGGAGCTG GGCAAGCTGC TGGCGGAGAT GCTGGTGCAG 
GCTACGCAGA TGCCATGCAG CCTGGACAAG CCGCATCGTT CCTCTCGGCT TGTCCCCGTG 
CTCTACCACG TGTATTCCTT CCGCAACGTC CAGCAGATCG GCATCCTGAA GCCGCACCCG 
GCCTACGTGC AGCTGCTGGA GAAGGCCGCG GAGCCCACGC TGACCTTCGA GGCGGTGGAT 
GTACCCATGC TTTGCCCCCC GCTGCCCTGG ACATCGCCGC ACTCTGGTGC TTTCCTGCTC 
AGCCCCACCA AGCTGATGCG CACGGTGGAA GGCGCCACGC AGCACCAGGA GCTGCTGGAA 
ACCTGCCCGC CCACCGCGCT GCATGGCGCA CTGGACGCCC TCACCCAACT GGGCAACTGC 
GCCTGGCGCG TCAACGGGCG CGTGCTGGAC CTGGTGCTGC AGCTCTTCCA GGCCAAGGGC 
TGCCCCCAGC TAGGCGTGCC GGCCCCGCCC TCCGAGGCGC CCCAGCCGCC CGAGGCCCAC 
CTGCCGCACA GCGCCGCGCC CGCCCGCAAG GCCGAGCTGC GCCGTGAGCT GGCGCACTGC 
CAGAAGGTGG CCCGGGAGAT GCACAGCCTG CGGGCGGAGG CGCTGTACCG CCTCTCGCTG 
GCGCAGCACC TGCGGGACCG CGTCTTCTGG CTGCCGCACA ACATGGACTT CCGCGGCCGC 
ACCTACCCCT GCCCGCCGCA CTTCAACCAC CTGGGCAGCG ACGTGGCGCG GGCCCTGCTG 
GAGTTCGCCC AGGGCCGCCC GCTCGGCCCG CACGGCCTGG ATTGGCTCAA GATCCACCTG 
GTCAATCTCA CGGGGTTGAA GAAGCGGGAG CCGCTGCGGA AGCGCCTGGC CTTTGCGGAG 
GAGGTGATGG ATGACATCCT GGACTCCGCG GACCAACCCT TGACGGGCCG AAAGTGGTGG 
ATGGGCGCGG AGGAACCCTG GCAGACGCTG GCCTGCTGTA TGGAGGTGGC GAACGCTGTG 
CGCGCCTCCG ACCCTGCCGC CTATGTCTCC CACCTCCCCG TCCATCAGGA CGGCTCTTGC 
AACGGCCTGC AGCATTATGC TGCTCTGGGC CGCGACAGCG TGGGCGCCGC CTCCGTCAAC 
CTGGAGCCCT CGGATGTGCC GCAGGACGTG TACAGCGGCG TGGCCGCGCA GGTGGAGGTG 
TTCCGTAGGC AGGACGCCCA GCGGGGCATG CGGGTGGCAC AGGTGCTGGA AGGTTTCATC 
ACCCGCAAGG TGGTGAAGCA GACGGTGATG ACGGTGGTGT ACGGGGTCAC GCGCTATGGC 
GGGCGCCTGC AGATTGAGAA GCGCCTCCGG GAGCTGAGCG ACTTTCCCCA GGAGTTCGTG 
TGGGAGGCCT CTCACTATCT CGTACGCCAG GTCTTCAAGA GTCTACAGGA GATGTTCTCG 
GGGACCCGGG CCATCCAGCA CTGGCTGACC GAGAGTGCCC GCCTCATCTC CCACATGGGC 
TCTGTGGTGG AGTGGGTCAC ACCCCTGGGC GTCCCCGTCA TCCAGCCCTA TCGCCTGGAC 
TCCAAGGTCA AGCAAATAGG AGGTGGAATT CAGAGCATCA CCTACACCCA CAACGGAGAC 
ATCAGCCGAA AGCCCAACAC ACGTAAGCAG AAGAACGGCT TCCCGCCCAA CTTCATCCAC 
TCGCTGGACT CCTCCCACAT GATGCTCACC GCCCTGCACT GCTACAGGAG TCCTCCGCTC 
TGCCACAGGA AGGGCCTGAC CTTCGTCTCT GTGCACGACT GTTACTGGAC TCACGCAGCT 
GATGTCTCCG TCATGAACCA GGTGTGCCGG GAGCAGTTTG TCCGCTTGCA CAGCGAGCCC 
ATCCTGCAGG ACCTGTCCAG ATTCCTGGTC AAGCGGTTCT GCTCTGAGCC CCAGAAGATC 
TTGGAGGCCA GCCAGCTGAA GGAGACACTG CAGGCGGTGC CCAAGCCAGG GGCCTTCGAC 
CTGGAGCAGG TGAAGCGTTC CACCTACTTC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Length heterogeneity at conserved sequence block 2 in human mitochondrial DNA acts as a rheostat for RNA polymerase POLRMT activity.
Nucleic acids research44(16)7817-29(2016 Sep)
Tan BG,Wellesley FC,Savery NJ,Szczelkun MD

Mitochondrial Ribosomal Protein L12 Is Required for POLRMT Stability and Exists as Two Forms Generated by Alternative Proteolysis during Import.
The Journal of biological chemistry291(2)989-97(2016 Jan)
Nouws J,Goswami AV,Bestwick M,McCann BJ,Surovtseva YV,Shadel GS

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POLRMT cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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