POLR2L cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLR2L
Entrez Gene ID	5441
Full Name	RNA polymerase II subunit L
Synonyms	RBP10,RPABC5,RPB10,RPB10beta,RPB7.6,hRPB7.6
General protein information	Preferred Names RNA polymerase II subunit L Names DNA-directed RNA polymerases I, II, and III subunit RPABC5 DNA-directed RNA polymerase III subunit L RNA polymerase II 7.6 kDa subunit RNA polymerases I, II, and III subunit ABC5 RPB10 homolog polymerase (RNA) II (DNA directed) polypeptide L, 7.6kDa polymerase (RNA) II subunit L
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p15.5
Summary	This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains four conserved cysteines characteristic of an atypical zinc-binding domain. Like its counterpart in yeast, this subunit may be shared by the other two DNA-directed RNA polymerases. [provided by RefSeq, Jul 2008].
Disorder MIM:	601189

mRNA and Protein(s)

mRNA	Protein	Name
NM_021128.4	NP_066951.1	DNA-directed RNA polymerases I, II, and III subunit RPABC5
NM_021128.5	NP_066951.1	DNA-directed RNA polymerases I, II, and III subunit RPABC5

Pathways from BioSystems

Homologies

Bos taurus (cattle)	POLR2L	NP_001107198.1
Drosophila melanogaster (fruit fly)	Rpb10	NP_651280.1
Caenorhabditis elegans (roundworm)	rpb-10	NP_490923.1
Kluyveromyces lactis	KLLA0D06699g	XP_453359.1
Schizosaccharomyces pombe (fission yeast)	rpb10	NP_594797.1
Magnaporthe oryzae (rice blast fungus)	MGG_15646	XP_003714124.1
Homo sapiens (human)	POLR2L	NP_066951.1
Pan troglodytes (chimpanzee)	POLR2L	XP_001151400.2
Rattus norvegicus (Norway rat)	LOC100911840	XP_003749068.1
Saccharomyces cerevisiae (baker's yeast)	RPB10	NP_014853.3
Neurospora crassa	NCU02605	XP_965008.1
Oryza sativa (rice)	Os11g0194800	NP_001067421.1
Mus musculus (house mouse)	Polr2l	NP_079869.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011761	XP_320749.2
	POLR2L	XP_001089261.1
Gallus gallus (chicken)	POLR2L	XP_001233059.3
Danio rerio (zebrafish)	polr2l	NP_001153297.1
Eremothecium gossypii	AGOS_ACR163W	NP_983565.1
Arabidopsis thaliana (thale cress)	NRPB10	NP_849640.1
Oryza sativa (rice)	Os12g0180400	NP_001066312.1
Xenopus tropicalis (tropical clawed frog)	polr2l.1	NP_001016343.1

Gene Ontology
Bibliography

Related articles in PubMed

A protein interaction framework for human mRNA degradation.
Lehner B, Sanderson CM
Genome research14(7)1315-23(2004 Jul)

A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway.
Bouwmeester T, Bauch A, Ruffner H, Angrand PO, Bergamini G, Croughton K, Cruciat C, Eberhard D, Gagneur J, Ghidelli S, Hopf C, Huhse B, Mangano R, Michon AM, Schirle M, Schlegl J, Schwab M, Stein MA, Bauer A, Casari G, Drewes G, Gavin AC, Jackson DB, Joberty G, Neubauer G, Rick J, Kuster B, Superti-Furga G
Nature cell biology6(2)97-105(2004 Feb)

The Tat/TAR-dependent phosphorylation of RNA polymerase II C-terminal domain stimulates cotranscriptional capping of HIV-1 mRNA.
Zhou M, Deng L, Kashanchi F, Brady JN, Shatkin AJ, Kumar A
Proceedings of the National Academy of Sciences of the United States of America100(22)12666-71(2003 Oct)

Acetylation of Tat defines a cyclinT1-independent step in HIV transactivation.
Kaehlcke K, Dorr A, Hetzer-Egger C, Kiermer V, Henklein P, Schnoelzer M, Loret E, Cole PA, Verdin E, Ott M
Molecular cell12(1)167-76(2003 Jul)

The gene (POLR2L) encoding the hRPB7.6 subunit of human RNA polymerase.
Acker J, Murroni O, Mattei MG, Kedinger C, Vigneron M
Genomics32(1)86-90(1996 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POLR2L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLR2L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu32032	NM_021128.5 Latest version!	Homo sapiens RNA polymerase II subunit L (POLR2L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu32032	NM_021128.4	Homo sapiens RNA polymerase II subunit L (POLR2L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLR2L ( NM_021128.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_066951.1 Homo sapiens RNA polymerase II subunit L (POLR2L), mRNA.

Price & Availability↑

CloneID	OHu32032
Clone ID Related Accession (Same CDS sequence)	NM_021128.4 , NM_021128.5
Accession Version	NM_021128.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerases I, II, and III subunit RPABC5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC005903.1, BC018649.2, BQ435190.1 and BU679804.1. On Jan 9, 2009 this sequence version replaced NM_021128.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.295353.1, SRR1163655.260693.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGATCATCC CTGTACGCTG CTTCACTTGT GGCAAGATCG TCGGCAACAA GTGGGAGGCT 
TACCTGGGGC TGCTGCAGGC CGAGTACACC GAGGGGGATG CGCTGGATGC CCTGGGCCTG 
AAGCGCTACT GCTGCCGCCG GATGCTGCTG GCCCACGTGG ACCTGATCGA GAAGCTGCTC 
AATTATGCAC CCCTGGAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066951.1
CDS	22..225
Translation	MIIPVRCFTCGKIVGNKWEAYLGLLQAEYTEGDALDALGLKRYCCRRMLLAHVDLIEKLLNYAPLEK

Target ORF information:

RefSeq Version	NM_021128.4
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA polymerase II subunit L (POLR2L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021128.4

ORF Insert Sequence:

ATGATCATCC CTGTACGCTG CTTCACTTGT GGCAAGATCG TCGGCAACAA GTGGGAGGCT 
TACCTGGGGC TGCTGCAGGC CGAGTACACC GAGGGGGATG CGCTGGATGC CCTGGGCCTG 
AAGCGCTACT GCTGCCGCCG GATGCTGCTG GCCCACGTGG ACCTGATCGA GAAGCTGCTC 
AATTATGCAC CCCTGGAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLR2L ( NM_021128.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_066951.1 Homo sapiens RNA polymerase II subunit L (POLR2L), mRNA.

Price & Availability↑

CloneID	OHu32032
Clone ID Related Accession (Same CDS sequence)	NM_021128.4 , NM_021128.5
Accession Version	NM_021128.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerases I, II, and III subunit RPABC5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC005903.1, BC018649.2, BQ435190.1 and BU679804.1. On Nov 23, 2018 this sequence version replaced NM_021128.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.295353.1, SRR5189667.157673.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000322028.5/ ENSP00000324124.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATCATCC CTGTACGCTG CTTCACTTGT GGCAAGATCG TCGGCAACAA GTGGGAGGCT 
TACCTGGGGC TGCTGCAGGC CGAGTACACC GAGGGGGATG CGCTGGATGC CCTGGGCCTG 
AAGCGCTACT GCTGCCGCCG GATGCTGCTG GCCCACGTGG ACCTGATCGA GAAGCTGCTC 
AATTATGCAC CCCTGGAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066951.1
CDS	22..225
Translation	MIIPVRCFTCGKIVGNKWEAYLGLLQAEYTEGDALDALGLKRYCCRRMLLAHVDLIEKLLNYAPLEK

Target ORF information:

RefSeq Version	NM_021128.5
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA polymerase II subunit L (POLR2L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021128.5

ORF Insert Sequence:

ATGATCATCC CTGTACGCTG CTTCACTTGT GGCAAGATCG TCGGCAACAA GTGGGAGGCT 
TACCTGGGGC TGCTGCAGGC CGAGTACACC GAGGGGGATG CGCTGGATGC CCTGGGCCTG 
AAGCGCTACT GCTGCCGCCG GATGCTGCTG GCCCACGTGG ACCTGATCGA GAAGCTGCTC 
AATTATGCAC CCCTGGAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A protein interaction framework for human mRNA degradation.
Genome research14(7)1315-23(2004 Jul)
Lehner B,Sanderson CM

A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway.
Nature cell biology6(2)97-105(2004 Feb)
Bouwmeester T,Bauch A,Ruffner H,Angrand PO,Bergamini G,Croughton K,Cruciat C,Eberhard D,Gagneur J,Ghidelli S,Hopf C,Huhse B,Mangano R,Michon AM,Schirle M,Schlegl J,Schwab M,Stein MA,Bauer A,Casari G,Drewes G,Gavin AC,Jackson DB,Joberty G,Neubauer G,Rick J,Kuster B,Superti-Furga G

The Tat/TAR-dependent phosphorylation of RNA polymerase II C-terminal domain stimulates cotranscriptional capping of HIV-1 mRNA.
Proceedings of the National Academy of Sciences of the United States of America100(22)12666-71(2003 Oct)
Zhou M,Deng L,Kashanchi F,Brady JN,Shatkin AJ,Kumar A

Acetylation of Tat defines a cyclinT1-independent step in HIV transactivation.
Molecular cell12(1)167-76(2003 Jul)
Kaehlcke K,Dorr A,Hetzer-Egger C,Kiermer V,Henklein P,Schnoelzer M,Loret E,Cole PA,Verdin E,Ott M

The gene (POLR2L) encoding the hRPB7.6 subunit of human RNA polymerase.
Genomics32(1)86-90(1996 Feb)
Acker J,Murroni O,Mattei MG,Kedinger C,Vigneron M

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POLR2L cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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