POLR2K cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLR2K
Entrez Gene ID	5440
Full Name	RNA polymerase II subunit K
Synonyms	ABC10-alpha,RPABC4,RPB10alpha,RPB12,RPB7.0,hRPB7.0,hsRPB10a
General protein information	Preferred Names RNA polymerase II subunit K Names DNA-directed RNA polymerases I, II, and III subunit RPABC4 DNA directed RNA polymerases I, II, and III 7.0 kda polypeptide DNA-directed RNA polymerase II subunit K RNA polymerase II 7.0 kDa subunit RNA polymerases I, II, and III subunit ABC4 polymerase (RNA) II (DNA directed) polypeptide K, 7.0kDa polymerase (RNA) II subunit K
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8q22.2
Summary	This gene encodes one of the smallest subunits of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases. [provided by RefSeq, Jul 2008].
Disorder MIM:	606033

mRNA and Protein(s)

mRNA	Protein	Name
NM_005034.4	NP_005025.1	DNA-directed RNA polymerases I, II, and III subunit RPABC4
NM_005034.3	NP_005025.1	DNA-directed RNA polymerases I, II, and III subunit RPABC4

Pathways from BioSystems

Homologies

Bos taurus (cattle)	POLR2K	NP_001032712.1
Mus musculus (house mouse)	Polr2k	NP_001034457.1
Xenopus tropicalis (tropical clawed frog)	polr2k	NP_001016509.1
Homo sapiens (human)	POLR2K	NP_005025.1
	POLR2K	XP_001096949.1
Danio rerio (zebrafish)	polr2k	NP_001186035.1
Pan troglodytes (chimpanzee)	POLR2K	XP_001151505.1
Canis lupus familiaris (dog)	POLR2K	XP_005627885.1
Rattus norvegicus (Norway rat)	Polr2k	NP_001269254.1

Gene Ontology
Bibliography

Related articles in PubMed

Single nucleotide polymorphisms in miRNA binding sites and miRNA genes as breast/ovarian cancer risk modifiers in Jewish high-risk women.
Kontorovich T, Levy A, Korostishevsky M, Nir U, Friedman E
International journal of cancer127(3)589-97(2010 Aug)

Genetic polymorphisms in 85 DNA repair genes and bladder cancer risk.
Michiels S, Laplanche A, Boulet T, Dessen P, Guillonneau B, M?jean A, Desgrandchamps F, Lathrop M, Sarasin A, Benhamou S
Carcinogenesis30(5)763-8(2009 May)

The NS5A protein of hepatitis C virus represses gene expression of hRPB10alpha, a common subunit of host RNA polymerases, through interferon regulatory factor-1 binding site.
Jung CR, Choi S, Im DS
Virus research129(2)155-65(2007 Nov)

SMYD3 encodes a histone methyltransferase involved in the proliferation of cancer cells.
Hamamoto R, Furukawa Y, Morita M, Iimura Y, Silva FP, Li M, Yagyu R, Nakamura Y
Nature cell biology6(8)731-40(2004 Aug)

The Tat/TAR-dependent phosphorylation of RNA polymerase II C-terminal domain stimulates cotranscriptional capping of HIV-1 mRNA.
Zhou M, Deng L, Kashanchi F, Brady JN, Shatkin AJ, Kumar A
Proceedings of the National Academy of Sciences of the United States of America100(22)12666-71(2003 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator)
Title: Single nucleotide polymorphisms in miRNA binding sites and miRNA genes as breast/ovarian cancer risk modifiers in Jewish high-risk women.

Observational study of gene-disease association, gene-gene interaction, and gene-environment interaction. (HuGE Navigator)
Title: Genetic polymorphisms in 85 DNA repair genes and bladder cancer risk.

results suggest that NS5A may partly modulate host cell transcription by the down-regulation of hRPB10alpha
Title: The NS5A protein of hepatitis C virus represses gene expression of hRPB10alpha, a common subunit of host RNA polymerases, through interferon regulatory factor-1 binding site.

The following POLR2K gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLR2K cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22384	NM_005034.4 Latest version!	Homo sapiens RNA polymerase II subunit K (POLR2K), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu22384	NM_005034.3	Homo sapiens RNA polymerase II subunit K (POLR2K), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLR2K ( NM_005034.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005025.1 Homo sapiens RNA polymerase II subunit K (POLR2K), mRNA.

Price & Availability↑

CloneID	OHu22384
Clone ID Related Accession (Same CDS sequence)	NM_005034.4 , NM_005034.3
Accession Version	NM_005034.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerases I, II, and III subunit RPABC4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC018157.1 and AC025647.9. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_005034.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189655.178233.1, SRR5189652.107793.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000353107.8/ ENSP00000342889.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121

ATGGACACCC AGAAGGACGT TCAACCTCCA AAGCAGCAAC CAATGATATA TATCTGTGGA 
GAGTGTCACA CAGAAAATGA AATAAAATCT AGGGATCCAA TCAGATGCAG AGAATGTGGA 
TACAGAATAA TGTACAAGAA AAGGACTAAA AGATTGGTCG TTTTTGATGC TCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005025.1
CDS	84..260
Translation	MDTQKDVQPPKQQPMIYICGECHTENEIKSRDPIRCRECGYRIMYKKRTKRLVVFDAR

Target ORF information:

RefSeq Version	NM_005034.4
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA polymerase II subunit K (POLR2K), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005034.4

ORF Insert Sequence:

1
61
121

ATGGACACCC AGAAGGACGT TCAACCTCCA AAGCAGCAAC CAATGATATA TATCTGTGGA 
GAGTGTCACA CAGAAAATGA AATAAAATCT AGGGATCCAA TCAGATGCAG AGAATGTGGA 
TACAGAATAA TGTACAAGAA AAGGACTAAA AGATTGGTCG TTTTTGATGC TCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLR2K ( NM_005034.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005025.1 Homo sapiens RNA polymerase II subunit K (POLR2K), mRNA.

Price & Availability↑

CloneID	OHu22384
Clone ID Related Accession (Same CDS sequence)	NM_005034.4 , NM_005034.3
Accession Version	NM_005034.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerases I, II, and III subunit RPABC4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC018157.1 and AC025647.9. This sequence is a reference standard in the RefSeqGene project. On Apr 8, 2005 this sequence version replaced NM_005034.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.36283.1, SRR1163657.278542.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121

ATGGACACCC AGAAGGACGT TCAACCTCCA AAGCAGCAAC CAATGATATA TATCTGTGGA 
GAGTGTCACA CAGAAAATGA AATAAAATCT AGGGATCCAA TCAGATGCAG AGAATGTGGA 
TACAGAATAA TGTACAAGAA AAGGACTAAA AGATTGGTCG TTTTTGATGC TCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005025.1
CDS	109..285
Translation	MDTQKDVQPPKQQPMIYICGECHTENEIKSRDPIRCRECGYRIMYKKRTKRLVVFDAR

Target ORF information:

RefSeq Version	NM_005034.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA polymerase II subunit K (POLR2K), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005034.3

ORF Insert Sequence:

1
61
121

ATGGACACCC AGAAGGACGT TCAACCTCCA AAGCAGCAAC CAATGATATA TATCTGTGGA 
GAGTGTCACA CAGAAAATGA AATAAAATCT AGGGATCCAA TCAGATGCAG AGAATGTGGA 
TACAGAATAA TGTACAAGAA AAGGACTAAA AGATTGGTCG TTTTTGATGC TCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Single nucleotide polymorphisms in miRNA binding sites and miRNA genes as breast/ovarian cancer risk modifiers in Jewish high-risk women.
International journal of cancer127(3)589-97(2010 Aug)
Kontorovich T,Levy A,Korostishevsky M,Nir U,Friedman E

Genetic polymorphisms in 85 DNA repair genes and bladder cancer risk.
Carcinogenesis30(5)763-8(2009 May)
Michiels S,Laplanche A,Boulet T,Dessen P,Guillonneau B,M?jean A,Desgrandchamps F,Lathrop M,Sarasin A,Benhamou S

The NS5A protein of hepatitis C virus represses gene expression of hRPB10alpha, a common subunit of host RNA polymerases, through interferon regulatory factor-1 binding site.
Virus research129(2)155-65(2007 Nov)
Jung CR,Choi S,Im DS

SMYD3 encodes a histone methyltransferase involved in the proliferation of cancer cells.
Nature cell biology6(8)731-40(2004 Aug)
Hamamoto R,Furukawa Y,Morita M,Iimura Y,Silva FP,Li M,Yagyu R,Nakamura Y

The Tat/TAR-dependent phosphorylation of RNA polymerase II C-terminal domain stimulates cotranscriptional capping of HIV-1 mRNA.
Proceedings of the National Academy of Sciences of the United States of America100(22)12666-71(2003 Oct)
Zhou M,Deng L,Kashanchi F,Brady JN,Shatkin AJ,Kumar A

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POLR2K cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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