POLR2G cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLR2G
Entrez Gene ID	5436
Full Name	RNA polymerase II subunit G
Synonyms	RPB19,RPB7,hRPB19,hsRPB7
General protein information	Preferred Names RNA polymerase II subunit G Names DNA-directed RNA polymerase II subunit RPB7 DNA directed RNA polymerase II 19 kda polypeptide DNA-directed RNA polymerase II 19 kDa polypeptide DNA-directed RNA polymerase II subunit G RNA polymerase II 19 kDa subunit RNA polymerase II seventh subunit RNA polymerase II subunit B7 polymerase (RNA) II (DNA directed) polypeptide G polymerase (RNA) II subunit G
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q12.3
Summary	This gene encodes the seventh largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The protein functions in transcription initiation, and is also thought to help stabilize transcribing polyermase molecules during elongation. [provided by RefSeq, Jan 2009].
Disorder MIM:	602013

mRNA and Protein(s)

mRNA	Protein	Name
NM_002696.3	NP_002687.1	DNA-directed RNA polymerase II subunit RPB7
NM_002696.2	NP_002687.1	DNA-directed RNA polymerase II subunit RPB7

Pathways from BioSystems

Homologies

Bos taurus (cattle)	POLR2G	NP_001015633.1
Mus musculus (house mouse)	Polr2g	NP_080605.1
Rattus norvegicus (Norway rat)	Polr2g	NP_446400.1
Saccharomyces cerevisiae (baker's yeast)	RPB7	NP_010692.3
Pan troglodytes (chimpanzee)	POLR2G	XP_001142605.2
Arabidopsis thaliana (thale cress)	NRPB7	NP_200726.1
Oryza sativa (rice)	Os05g0400700	NP_001055483.1
	POLR2G	XP_001116161.1
Danio rerio (zebrafish)	polr2gl	NP_955963.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002121	XP_320916.2
Caenorhabditis elegans (roundworm)	rpb-7	NP_491093.1
Homo sapiens (human)	POLR2G	NP_002687.1
Canis lupus familiaris (dog)	POLR2G	XP_533260.1
Schizosaccharomyces pombe (fission yeast)	rpb7	XP_001713072.1
Xenopus tropicalis (tropical clawed frog)	polr2g	NP_001017065.1
Drosophila melanogaster (fruit fly)	Rpb7	NP_731983.1
Kluyveromyces lactis	KLLA0C06787g	XP_452500.1
Eremothecium gossypii	AGOS_AFR402C	NP_985949.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

The RNA Pol II sub-complex hsRpb4/7 is required for viability of multiple human cell lines.
Zhao Y, Li KK, Ng KP, Ng CH, Lee KA
Protein & cell3(11)846-54(2012 Nov)

Genomic location of the human RNA polymerase II general machinery: evidence for a role of TFIIF and Rpb7 at both early and late stages of transcription.
Cojocaru M, Jeronimo C, Forget D, Bouchard A, Bergeron D, C?te P, Poirier GG, Greenblatt J, Coulombe B
The Biochemical journal409(1)139-47(2008 Jan)

RNA emerging from the active site of RNA polymerase II interacts with the Rpb7 subunit.
Ujv?ri A, Luse DS
Nature structural & molecular biology13(1)49-54(2006 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The function of Rpb7 interaction with rpb4 in human cells indicate that Rpb7 have gene-specific effects but are also more generally required for human cell survival.
Title: The RNA Pol II sub-complex hsRpb4/7 is required for viability of multiple human cell lines.

TFIIF and Rpb7 are involved in both early and late transcriptional stages
Title: Genomic location of the human RNA polymerase II general machinery: evidence for a role of TFIIF and Rpb7 at both early and late stages of transcription.

the emerging transcript cross-links to the Rpb7 subunit of RNA polymerase II in various complexes containing 26- to 32-nt transcripts
Title: RNA emerging from the active site of RNA polymerase II interacts with the Rpb7 subunit.

Identification of the RNA binding region of Rpb7.
Title: Crystal structure and RNA binding of the Rpb4/Rpb7 subunits of human RNA polymerase II.

The following POLR2G gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLR2G cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31758	NM_002696.3 Latest version!	Homo sapiens RNA polymerase II subunit G (POLR2G), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu31758	NM_002696.2	Homo sapiens RNA polymerase II subunit G (POLR2G), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLR2G ( NM_002696.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002687.1 Homo sapiens RNA polymerase II subunit G (POLR2G), mRNA.

Price & Availability↑

CloneID	OHu31758
Clone ID Related Accession (Same CDS sequence)	NM_002696.3 , NM_002696.2
Accession Version	NM_002696.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerase II subunit RPB7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG530809.1, BC112162.1 and AW016747.1. On Nov 22, 2018 this sequence version replaced NM_002696.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.238328.1, SRR1163655.390074.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000301788.12/ ENSP00000301788.7 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTCTACC ATATCTCCCT AGAGCACGAA ATCCTGCTGC ACCCGCGCTA CTTCGGCCCC 
AACTTGCTCA ACACGGTGAA GCAGAAGCTC TTCACCGAGG TGGAGGGGAC CTGCACAGGG 
AAGTATGGCT TTGTAATTGC TGTCACCACC ATTGACAATA TTGGTGCTGG TGTGATCCAG 
CCAGGCCGAG GCTTTGTCCT TTATCCAGTT AAGTACAAGG CCATTGTTTT CCGGCCATTT 
AAAGGGGAGG TCGTGGATGC TGTTGTCACT CAGGTCAACA AGGTTGGACT CTTCACAGAA 
ATTGGGCCCA TGTCTTGCTT CATCTCTCGA CATTCCATCC CTTCAGAGAT GGAGTTTGAT 
CCTAACTCCA ACCCACCATG TTACAAGACA ATGGATGAGG ATATTGTGAT TCAGCAGGAC 
GATGAGATCC GCTTAAAGAT TGTGGGGACC CGTGTGGACA AGAATGACAT TTTTGCTATT 
GGCTCCCTGA TGGACGATTA CTTGGGGCTT GTAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002687.1
CDS	70..588
Translation	MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQPGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFDPNSNPPCYKTMDEDIVIQQDDEIRLKIVGTRVDKNDIFAIGSLMDDYLGLVS

Target ORF information:

RefSeq Version	NM_002696.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA polymerase II subunit G (POLR2G), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002696.3

ORF Insert Sequence:

ATGTTCTACC ATATCTCCCT AGAGCACGAA ATCCTGCTGC ACCCGCGCTA CTTCGGCCCC 
AACTTGCTCA ACACGGTGAA GCAGAAGCTC TTCACCGAGG TGGAGGGGAC CTGCACAGGG 
AAGTATGGCT TTGTAATTGC TGTCACCACC ATTGACAATA TTGGTGCTGG TGTGATCCAG 
CCAGGCCGAG GCTTTGTCCT TTATCCAGTT AAGTACAAGG CCATTGTTTT CCGGCCATTT 
AAAGGGGAGG TCGTGGATGC TGTTGTCACT CAGGTCAACA AGGTTGGACT CTTCACAGAA 
ATTGGGCCCA TGTCTTGCTT CATCTCTCGA CATTCCATCC CTTCAGAGAT GGAGTTTGAT 
CCTAACTCCA ACCCACCATG TTACAAGACA ATGGATGAGG ATATTGTGAT TCAGCAGGAC 
GATGAGATCC GCTTAAAGAT TGTGGGGACC CGTGTGGACA AGAATGACAT TTTTGCTATT 
GGCTCCCTGA TGGACGATTA CTTGGGGCTT GTAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLR2G ( NM_002696.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_002687.1 Homo sapiens RNA polymerase II subunit G (POLR2G), mRNA.

Price & Availability↑

CloneID	OHu31758
Clone ID Related Accession (Same CDS sequence)	NM_002696.3 , NM_002696.2
Accession Version	NM_002696.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerase II subunit RPB7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG530809.1, BC112162.1 and AW016747.1. On Jan 9, 2009 this sequence version replaced NM_002696.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.38805.1, SRR1163655.492628.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCTACC ATATCTCCCT AGAGCACGAA ATCCTGCTGC ACCCGCGCTA CTTCGGCCCC 
AACTTGCTCA ACACGGTGAA GCAGAAGCTC TTCACCGAGG TGGAGGGGAC CTGCACAGGG 
AAGTATGGCT TTGTAATTGC TGTCACCACC ATTGACAATA TTGGTGCTGG TGTGATCCAG 
CCAGGCCGAG GCTTTGTCCT TTATCCAGTT AAGTACAAGG CCATTGTTTT CCGGCCATTT 
AAAGGGGAGG TCGTGGATGC TGTTGTCACT CAGGTCAACA AGGTTGGACT CTTCACAGAA 
ATTGGGCCCA TGTCTTGCTT CATCTCTCGA CATTCCATCC CTTCAGAGAT GGAGTTTGAT 
CCTAACTCCA ACCCACCATG TTACAAGACA ATGGATGAGG ATATTGTGAT TCAGCAGGAC 
GATGAGATCC GCTTAAAGAT TGTGGGGACC CGTGTGGACA AGAATGACAT TTTTGCTATT 
GGCTCCCTGA TGGACGATTA CTTGGGGCTT GTAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002687.1
CDS	111..629
Translation	MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQPGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFDPNSNPPCYKTMDEDIVIQQDDEIRLKIVGTRVDKNDIFAIGSLMDDYLGLVS

Target ORF information:

RefSeq Version	NM_002696.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA polymerase II subunit G (POLR2G), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002696.2

ORF Insert Sequence:

ATGTTCTACC ATATCTCCCT AGAGCACGAA ATCCTGCTGC ACCCGCGCTA CTTCGGCCCC 
AACTTGCTCA ACACGGTGAA GCAGAAGCTC TTCACCGAGG TGGAGGGGAC CTGCACAGGG 
AAGTATGGCT TTGTAATTGC TGTCACCACC ATTGACAATA TTGGTGCTGG TGTGATCCAG 
CCAGGCCGAG GCTTTGTCCT TTATCCAGTT AAGTACAAGG CCATTGTTTT CCGGCCATTT 
AAAGGGGAGG TCGTGGATGC TGTTGTCACT CAGGTCAACA AGGTTGGACT CTTCACAGAA 
ATTGGGCCCA TGTCTTGCTT CATCTCTCGA CATTCCATCC CTTCAGAGAT GGAGTTTGAT 
CCTAACTCCA ACCCACCATG TTACAAGACA ATGGATGAGG ATATTGTGAT TCAGCAGGAC 
GATGAGATCC GCTTAAAGAT TGTGGGGACC CGTGTGGACA AGAATGACAT TTTTGCTATT 
GGCTCCCTGA TGGACGATTA CTTGGGGCTT GTAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

The RNA Pol II sub-complex hsRpb4/7 is required for viability of multiple human cell lines.
Protein & cell3(11)846-54(2012 Nov)
Zhao Y,Li KK,Ng KP,Ng CH,Lee KA

Genomic location of the human RNA polymerase II general machinery: evidence for a role of TFIIF and Rpb7 at both early and late stages of transcription.
The Biochemical journal409(1)139-47(2008 Jan)
Cojocaru M,Jeronimo C,Forget D,Bouchard A,Bergeron D,C?te P,Poirier GG,Greenblatt J,Coulombe B

RNA emerging from the active site of RNA polymerase II interacts with the Rpb7 subunit.
Nature structural & molecular biology13(1)49-54(2006 Jan)
Ujv?ri A,Luse DS

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POLR2G cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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