POLD2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLD2
Entrez Gene ID	5425
Full Name	DNA polymerase delta 2, accessory subunit
General protein information	Preferred Names DNA polymerase delta 2, accessory subunit Names DNA polymerase delta subunit 2 DAN polymerase delta 2, accessory subunit DNA polymerase delta subunit p50 Pol delta B subunit (p50) polymerase (DNA directed), delta 2, accessory subunit polymerase (DNA directed), delta 2, regulatory subunit 50kDa polymerase (DNA) delta 2, accessory subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7p13
Summary	This gene encodes the 50-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein is required for the stimulation of DNA polymerase delta activity by the processivity cofactor proliferating cell nuclear antigen (PCNA). Expression of this gene may be a marker for ovarian carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Mar 2012].
Disorder MIM:	600815

mRNA and Protein(s)

mRNA	Protein	Name
XM_011515431.1	XP_011513733.1	DNA polymerase delta subunit 2 isoform X1
NM_001256879.1	NP_001243808.1	DNA polymerase delta subunit 2 isoform 1
NM_001127218.2	NP_001120690.1	DNA polymerase delta subunit 2 isoform 1
XM_024446802.1	XP_024302570.1	DNA polymerase delta subunit 2 isoform X1
NM_006230.3	NP_006221.2	DNA polymerase delta subunit 2 isoform 2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	POLD2	XP_004951044.1
Oryza sativa (rice)	Os03g0128500	NP_001048841.1
Canis lupus familiaris (dog)	POLD2	XP_539802.3
Mus musculus (house mouse)	Pold2	NP_032920.2
Danio rerio (zebrafish)	pold2	NP_998468.1
Drosophila melanogaster (fruit fly)	CG12018	NP_647659.1
Homo sapiens (human)	POLD2	NP_006221.2
Pan troglodytes (chimpanzee)	POLD2	NP_001233397.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008910	XP_319657.4
Xenopus tropicalis (tropical clawed frog)	pold2	NP_001005794.1
	POLD2	XP_001094281.2
Bos taurus (cattle)	POLD2	NP_776853.2
Rattus norvegicus (Norway rat)	Pold2	NP_001013068.1
Arabidopsis thaliana (thale cress)	POLD2	NP_973664.1

Gene Ontology
Bibliography

Related articles in PubMed

Human DNA polymerase delta double-mutant D316A;E318A interferes with DNA mismatch repair in vitro.
Liu D, Frederiksen JH, Liberti SE, L?tzen A, Keijzers G, Pena-Diaz J, Rasmussen LJ
Nucleic acids research45(16)9427-9440(2017 Sep)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

DNA Polymerases as targets for gene therapy of hepatocellular carcinoma.
Liu H, Wei Q, Wang J, Huang X, Li C, Zheng Q, Cao J, Jia Z
BMC cancer15325(2015 Apr)

POLD2 and KSP37 (FGFBP2) correlate strongly with histology, stage and outcome in ovarian carcinomas.
Elgaaen BV, Haug KB, Wang J, Olstad OK, Fortunati D, Onsrud M, Staff AC, Sauer T, Gautvik KM
PloS one5(11)e13837(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The proofreading activity of DNA polymerase delta plays a role in shunting DNA mismatch repair to an EXO1-dependent excision pathway as opposed to directly participating in gap formation via its 3'-5' exonuclease activity.
Title: Human DNA polymerase delta double-mutant D316A;E318A interferes with DNA mismatch repair in vitro.

Inhibition of DNA polymerases a, delra and e by AFP promoter-driven artificial microRNAs may lead to effective growth arrest of AFP-positive HCC cells,as novel strategy for gene therapy
Title: DNA Polymerases as targets for gene therapy of hepatocellular carcinoma.

The results show that the FF483-484 amino acids in the human Poleta (designated F1 motif) are necessary for the interaction of this polymerase with POLD2, the B subunit of the replicative DNA polymerase delta, both in vitro and in vivo.
Title: FF483-484 motif of human Pol? mediates its interaction with the POLD2 subunit of Pol? and contributes to DNA damage tolerance.

Results suggest that POLD2 and KSP37 might be potential prognostic biomarkers.
Title: POLD2 and KSP37 (FGFBP2) correlate strongly with histology, stage and outcome in ovarian carcinomas.

DNA polymerase delta catalytic subunit p125 induced by mutant type p53 is associated with hepatocellular carcinoma invasion.
Title: Significance of DNA polymerase delta catalytic subunit p125 induced by mutant p53 in the invasive potential of human hepatocellular carcinoma.

The following POLD2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLD2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02978	NM_001127218.2 Latest version!	Homo sapiens DNA polymerase delta 2, accessory subunit (POLD2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu02978	NM_001256879.1 Latest version!	Homo sapiens DNA polymerase delta 2, accessory subunit (POLD2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu02978	XM_024446802.1 Latest version!	Homo sapiens DNA polymerase delta 2, accessory subunit (POLD2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu05462	NM_006230.3 Latest version!	Homo sapiens DNA polymerase delta 2, accessory subunit (POLD2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu59807	XM_011515431.1 Latest version!	Homo sapiens polymerase (DNA) delta 2, accessory subunit (POLD2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLD2 ( NM_001256879.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243808.1 Homo sapiens DNA polymerase delta 2, accessory subunit (POLD2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu02978
Clone ID Related Accession (Same CDS sequence)	NM_001256879.1 , NM_001127218.2 , XM_024446802.1
Accession Version	NM_001256879.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	DNA polymerase delta subunit 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292347.1, DB025069.1, BC000459.2 and AW732014.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1 and 3 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292347.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTTCTG AGCAGGCTGC CCAGAGGGCC CACACTCTAC TGTCCCCACC ATCAGCCAAC 
AATGCCACCT TTGCCCGGGT GCCAGTGGCA ACCTACACCA ACTCCTCACA ACCCTTCCGG 
CTAGGAGAGC GCAGCTTTAG CCGGCAGTAT GCCCACATTT ATGCCACCCG CCTCATCCAA 
ATGAGACCCT TCCTGGAGAA CCGGGCCCAG CAGCACTGGG GCAGTGGAGT GGGAGTGAAG 
AAGCTGTGTG AACTGCAGCC TGAGGAGAAG TGCTGTGTGG TGGGCACTCT GTTCAAGGCC 
ATGCCGCTGC AGCCCTCCAT CCTGCGGGAG GTCAGCGAGG AGCACAACCT GCTCCCCCAG 
CCTCCTCGGA GTAAATACAT ACACCCAGAT GACGAGCTGG TCTTGGAAGA TGAACTGCAG 
CGTATCAAAC TAAAAGGCAC CATTGACGTG TCAAAGCTGG TTACGGGGAC TGTCCTGGCT 
GTGTTTGGCT CCGTGAGAGA CGACGGGAAG TTTCTGGTGG AGGACTATTG CTTTGCTGAC 
CTTGCTCCCC AGAAGCCCGC ACCCCCACTT GACACAGATA GGTTTGTGCT ACTGGTGTCC 
GGCCTGGGCC TGGGTGGCGG TGGAGGCGAG AGCCTGCTGG GCACCCAGCT GCTGGTGGAT 
GTGGTGACGG GGCAGCTTGG GGACGAAGGG GAGCAGTGCA GCGCCGCCCA CGTCTCCCGG 
GTTATCCTCG CTGGCAACCT CCTCAGCCAC AGCACCCAGA GCAGGGATTC TATCAATAAG 
GCCAAATACC TCACCAAGAA AACCCAGGCA GCCAGCGTGG AGGCTGTTAA GATGCTGGAT 
GAGATCCTCC TGCAGCTGAG CGCCTCAGTG CCCGTGGACG TGATGCCAGG CGAGTTTGAT 
CCCACCAATT ACACGCTCCC CCAGCAGCCC CTCCACCCCT GCATGTTCCC GCTGGCCACT 
GCCTACTCCA CGCTCCAGCT GGTCACCAAC CCCTACCAGG CCACCATTGA TGGAGTCAGA 
TTTTTGGGGA CATCAGGACA GAACGTGAGT GACATTTTCC GATACAGCAG CATGGAGGAT 
CACTTGGAGA TCCTGGAGTG GACCCTGCGG GTCCGTCACA TCAGCCCCAC AGCCCCTGAC 
ACTCTAGGTT GTTACCCCTT CTACAAAACT GACCCGTTCA TCTTCCCAGA GTGCCCGCAT 
GTCTACTTTT GTGGCAACAC CCCCAGCTTT GGCTCCAAAA TCATCCGAGG TCCTGAGGAC 
CAGACAGTGC TGTTGGTGAC TGTCCCTGAC TTCAGTGCCA CGCAGACCGC CTGCCTTGTG 
AACCTGCGCA GCCTGGCCTG CCAGCCCATC AGCTTCTCGG GCTTCGGGGC AGAGGACGAT 
GACCTGGGAG GCCTGGGGCT GGGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243808.1
CDS	651..2060
Translation	MFSEQAAQRAHTLLSPPSANNATFARVPVATYTNSSQPFRLGERSFSRQYAHIYATRLIQMRPFLENRAQQHWGSGVGVKKLCELQPEEKCCVVGTLFKAMPLQPSILREVSEEHNLLPQPPRSKYIHPDDELVLEDELQRIKLKGTIDVSKLVTGTVLAVFGSVRDDGKFLVEDYCFADLAPQKPAPPLDTDRFVLLVSGLGLGGGGGESLLGTQLLVDVVTGQLGDEGEQCSAAHVSRVILAGNLLSHSTQSRDSINKAKYLTKKTQAASVEAVKMLDEILLQLSASVPVDVMPGEFDPTNYTLPQQPLHPCMFPLATAYSTLQLVTNPYQATIDGVRFLGTSGQNVSDIFRYSSMEDHLEILEWTLRVRHISPTAPDTLGCYPFYKTDPFIFPECPHVYFCGNTPSFGSKIIRGPEDQTVLLVTVPDFSATQTACLVNLRSLACQPISFSGFGAEDDDLGGLGLGP

Target ORF information:

RefSeq Version	NM_001256879.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase delta 2, accessory subunit (POLD2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256879.1

ORF Insert Sequence:

ATGTTTTCTG AGCAGGCTGC CCAGAGGGCC CACACTCTAC TGTCCCCACC ATCAGCCAAC 
AATGCCACCT TTGCCCGGGT GCCAGTGGCA ACCTACACCA ACTCCTCACA ACCCTTCCGG 
CTAGGAGAGC GCAGCTTTAG CCGGCAGTAT GCCCACATTT ATGCCACCCG CCTCATCCAA 
ATGAGACCCT TCCTGGAGAA CCGGGCCCAG CAGCACTGGG GCAGTGGAGT GGGAGTGAAG 
AAGCTGTGTG AACTGCAGCC TGAGGAGAAG TGCTGTGTGG TGGGCACTCT GTTCAAGGCC 
ATGCCGCTGC AGCCCTCCAT CCTGCGGGAG GTCAGCGAGG AGCACAACCT GCTCCCCCAG 
CCTCCTCGGA GTAAATACAT ACACCCAGAT GACGAGCTGG TCTTGGAAGA TGAACTGCAG 
CGTATCAAAC TAAAAGGCAC CATTGACGTG TCAAAGCTGG TTACGGGGAC TGTCCTGGCT 
GTGTTTGGCT CCGTGAGAGA CGACGGGAAG TTTCTGGTGG AGGACTATTG CTTTGCTGAC 
CTTGCTCCCC AGAAGCCCGC ACCCCCACTT GACACAGATA GGTTTGTGCT ACTGGTGTCC 
GGCCTGGGCC TGGGTGGCGG TGGAGGCGAG AGCCTGCTGG GCACCCAGCT GCTGGTGGAT 
GTGGTGACGG GGCAGCTTGG GGACGAAGGG GAGCAGTGCA GCGCCGCCCA CGTCTCCCGG 
GTTATCCTCG CTGGCAACCT CCTCAGCCAC AGCACCCAGA GCAGGGATTC TATCAATAAG 
GCCAAATACC TCACCAAGAA AACCCAGGCA GCCAGCGTGG AGGCTGTTAA GATGCTGGAT 
GAGATCCTCC TGCAGCTGAG CGCCTCAGTG CCCGTGGACG TGATGCCAGG CGAGTTTGAT 
CCCACCAATT ACACGCTCCC CCAGCAGCCC CTCCACCCCT GCATGTTCCC GCTGGCCACT 
GCCTACTCCA CGCTCCAGCT GGTCACCAAC CCCTACCAGG CCACCATTGA TGGAGTCAGA 
TTTTTGGGGA CATCAGGACA GAACGTGAGT GACATTTTCC GATACAGCAG CATGGAGGAT 
CACTTGGAGA TCCTGGAGTG GACCCTGCGG GTCCGTCACA TCAGCCCCAC AGCCCCTGAC 
ACTCTAGGTT GTTACCCCTT CTACAAAACT GACCCGTTCA TCTTCCCAGA GTGCCCGCAT 
GTCTACTTTT GTGGCAACAC CCCCAGCTTT GGCTCCAAAA TCATCCGAGG TCCTGAGGAC 
CAGACAGTGC TGTTGGTGAC TGTCCCTGAC TTCAGTGCCA CGCAGACCGC CTGCCTTGTG 
AACCTGCGCA GCCTGGCCTG CCAGCCCATC AGCTTCTCGG GCTTCGGGGC AGAGGACGAT 
GACCTGGGAG GCCTGGGGCT GGGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLD2 ( NM_001127218.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001120690.1 Homo sapiens DNA polymerase delta 2, accessory subunit (POLD2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02978
Clone ID Related Accession (Same CDS sequence)	NM_001256879.1 , NM_001127218.2 , XM_024446802.1
Accession Version	NM_001127218.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	DNA polymerase delta subunit 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP224472.1, AK312294.1, BC000459.2 and AW732014.1. On Mar 2, 2012 this sequence version replaced NM_001127218.1. Transcript Variant: This variant (1) encodes isoform 1. Variants 1 and 3 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.226045.1, SRR1163658.129501.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTTCTG AGCAGGCTGC CCAGAGGGCC CACACTCTAC TGTCCCCACC ATCAGCCAAC 
AATGCCACCT TTGCCCGGGT GCCAGTGGCA ACCTACACCA ACTCCTCACA ACCCTTCCGG 
CTAGGAGAGC GCAGCTTTAG CCGGCAGTAT GCCCACATTT ATGCCACCCG CCTCATCCAA 
ATGAGACCCT TCCTGGAGAA CCGGGCCCAG CAGCACTGGG GCAGTGGAGT GGGAGTGAAG 
AAGCTGTGTG AACTGCAGCC TGAGGAGAAG TGCTGTGTGG TGGGCACTCT GTTCAAGGCC 
ATGCCGCTGC AGCCCTCCAT CCTGCGGGAG GTCAGCGAGG AGCACAACCT GCTCCCCCAG 
CCTCCTCGGA GTAAATACAT ACACCCAGAT GACGAGCTGG TCTTGGAAGA TGAACTGCAG 
CGTATCAAAC TAAAAGGCAC CATTGACGTG TCAAAGCTGG TTACGGGGAC TGTCCTGGCT 
GTGTTTGGCT CCGTGAGAGA CGACGGGAAG TTTCTGGTGG AGGACTATTG CTTTGCTGAC 
CTTGCTCCCC AGAAGCCCGC ACCCCCACTT GACACAGATA GGTTTGTGCT ACTGGTGTCC 
GGCCTGGGCC TGGGTGGCGG TGGAGGCGAG AGCCTGCTGG GCACCCAGCT GCTGGTGGAT 
GTGGTGACGG GGCAGCTTGG GGACGAAGGG GAGCAGTGCA GCGCCGCCCA CGTCTCCCGG 
GTTATCCTCG CTGGCAACCT CCTCAGCCAC AGCACCCAGA GCAGGGATTC TATCAATAAG 
GCCAAATACC TCACCAAGAA AACCCAGGCA GCCAGCGTGG AGGCTGTTAA GATGCTGGAT 
GAGATCCTCC TGCAGCTGAG CGCCTCAGTG CCCGTGGACG TGATGCCAGG CGAGTTTGAT 
CCCACCAATT ACACGCTCCC CCAGCAGCCC CTCCACCCCT GCATGTTCCC GCTGGCCACT 
GCCTACTCCA CGCTCCAGCT GGTCACCAAC CCCTACCAGG CCACCATTGA TGGAGTCAGA 
TTTTTGGGGA CATCAGGACA GAACGTGAGT GACATTTTCC GATACAGCAG CATGGAGGAT 
CACTTGGAGA TCCTGGAGTG GACCCTGCGG GTCCGTCACA TCAGCCCCAC AGCCCCTGAC 
ACTCTAGGTT GTTACCCCTT CTACAAAACT GACCCGTTCA TCTTCCCAGA GTGCCCGCAT 
GTCTACTTTT GTGGCAACAC CCCCAGCTTT GGCTCCAAAA TCATCCGAGG TCCTGAGGAC 
CAGACAGTGC TGTTGGTGAC TGTCCCTGAC TTCAGTGCCA CGCAGACCGC CTGCCTTGTG 
AACCTGCGCA GCCTGGCCTG CCAGCCCATC AGCTTCTCGG GCTTCGGGGC AGAGGACGAT 
GACCTGGGAG GCCTGGGGCT GGGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120690.1
CDS	290..1699
Translation	MFSEQAAQRAHTLLSPPSANNATFARVPVATYTNSSQPFRLGERSFSRQYAHIYATRLIQMRPFLENRAQQHWGSGVGVKKLCELQPEEKCCVVGTLFKAMPLQPSILREVSEEHNLLPQPPRSKYIHPDDELVLEDELQRIKLKGTIDVSKLVTGTVLAVFGSVRDDGKFLVEDYCFADLAPQKPAPPLDTDRFVLLVSGLGLGGGGGESLLGTQLLVDVVTGQLGDEGEQCSAAHVSRVILAGNLLSHSTQSRDSINKAKYLTKKTQAASVEAVKMLDEILLQLSASVPVDVMPGEFDPTNYTLPQQPLHPCMFPLATAYSTLQLVTNPYQATIDGVRFLGTSGQNVSDIFRYSSMEDHLEILEWTLRVRHISPTAPDTLGCYPFYKTDPFIFPECPHVYFCGNTPSFGSKIIRGPEDQTVLLVTVPDFSATQTACLVNLRSLACQPISFSGFGAEDDDLGGLGLGP

Target ORF information:

RefSeq Version	NM_001127218.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase delta 2, accessory subunit (POLD2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127218.2

ORF Insert Sequence:

ATGTTTTCTG AGCAGGCTGC CCAGAGGGCC CACACTCTAC TGTCCCCACC ATCAGCCAAC 
AATGCCACCT TTGCCCGGGT GCCAGTGGCA ACCTACACCA ACTCCTCACA ACCCTTCCGG 
CTAGGAGAGC GCAGCTTTAG CCGGCAGTAT GCCCACATTT ATGCCACCCG CCTCATCCAA 
ATGAGACCCT TCCTGGAGAA CCGGGCCCAG CAGCACTGGG GCAGTGGAGT GGGAGTGAAG 
AAGCTGTGTG AACTGCAGCC TGAGGAGAAG TGCTGTGTGG TGGGCACTCT GTTCAAGGCC 
ATGCCGCTGC AGCCCTCCAT CCTGCGGGAG GTCAGCGAGG AGCACAACCT GCTCCCCCAG 
CCTCCTCGGA GTAAATACAT ACACCCAGAT GACGAGCTGG TCTTGGAAGA TGAACTGCAG 
CGTATCAAAC TAAAAGGCAC CATTGACGTG TCAAAGCTGG TTACGGGGAC TGTCCTGGCT 
GTGTTTGGCT CCGTGAGAGA CGACGGGAAG TTTCTGGTGG AGGACTATTG CTTTGCTGAC 
CTTGCTCCCC AGAAGCCCGC ACCCCCACTT GACACAGATA GGTTTGTGCT ACTGGTGTCC 
GGCCTGGGCC TGGGTGGCGG TGGAGGCGAG AGCCTGCTGG GCACCCAGCT GCTGGTGGAT 
GTGGTGACGG GGCAGCTTGG GGACGAAGGG GAGCAGTGCA GCGCCGCCCA CGTCTCCCGG 
GTTATCCTCG CTGGCAACCT CCTCAGCCAC AGCACCCAGA GCAGGGATTC TATCAATAAG 
GCCAAATACC TCACCAAGAA AACCCAGGCA GCCAGCGTGG AGGCTGTTAA GATGCTGGAT 
GAGATCCTCC TGCAGCTGAG CGCCTCAGTG CCCGTGGACG TGATGCCAGG CGAGTTTGAT 
CCCACCAATT ACACGCTCCC CCAGCAGCCC CTCCACCCCT GCATGTTCCC GCTGGCCACT 
GCCTACTCCA CGCTCCAGCT GGTCACCAAC CCCTACCAGG CCACCATTGA TGGAGTCAGA 
TTTTTGGGGA CATCAGGACA GAACGTGAGT GACATTTTCC GATACAGCAG CATGGAGGAT 
CACTTGGAGA TCCTGGAGTG GACCCTGCGG GTCCGTCACA TCAGCCCCAC AGCCCCTGAC 
ACTCTAGGTT GTTACCCCTT CTACAAAACT GACCCGTTCA TCTTCCCAGA GTGCCCGCAT 
GTCTACTTTT GTGGCAACAC CCCCAGCTTT GGCTCCAAAA TCATCCGAGG TCCTGAGGAC 
CAGACAGTGC TGTTGGTGAC TGTCCCTGAC TTCAGTGCCA CGCAGACCGC CTGCCTTGTG 
AACCTGCGCA GCCTGGCCTG CCAGCCCATC AGCTTCTCGG GCTTCGGGGC AGAGGACGAT 
GACCTGGGAG GCCTGGGGCT GGGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLD2 ( XM_024446802.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024302570.1 Homo sapiens DNA polymerase delta 2, accessory subunit (POLD2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu02978
Clone ID Related Accession (Same CDS sequence)	NM_001256879.1 , NM_001127218.2 , XM_024446802.1
Accession Version	XM_024446802.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DNA polymerase delta subunit 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTCTG AGCAGGCTGC CCAGAGGGCC CACACTCTAC TGTCCCCACC ATCAGCCAAC 
AATGCCACCT TTGCCCGGGT GCCAGTGGCA ACCTACACCA ACTCCTCACA ACCCTTCCGG 
CTAGGAGAGC GCAGCTTTAG CCGGCAGTAT GCCCACATTT ATGCCACCCG CCTCATCCAA 
ATGAGACCCT TCCTGGAGAA CCGGGCCCAG CAGCACTGGG GCAGTGGAGT GGGAGTGAAG 
AAGCTGTGTG AACTGCAGCC TGAGGAGAAG TGCTGTGTGG TGGGCACTCT GTTCAAGGCC 
ATGCCGCTGC AGCCCTCCAT CCTGCGGGAG GTCAGCGAGG AGCACAACCT GCTCCCCCAG 
CCTCCTCGGA GTAAATACAT ACACCCAGAT GACGAGCTGG TCTTGGAAGA TGAACTGCAG 
CGTATCAAAC TAAAAGGCAC CATTGACGTG TCAAAGCTGG TTACGGGGAC TGTCCTGGCT 
GTGTTTGGCT CCGTGAGAGA CGACGGGAAG TTTCTGGTGG AGGACTATTG CTTTGCTGAC 
CTTGCTCCCC AGAAGCCCGC ACCCCCACTT GACACAGATA GGTTTGTGCT ACTGGTGTCC 
GGCCTGGGCC TGGGTGGCGG TGGAGGCGAG AGCCTGCTGG GCACCCAGCT GCTGGTGGAT 
GTGGTGACGG GGCAGCTTGG GGACGAAGGG GAGCAGTGCA GCGCCGCCCA CGTCTCCCGG 
GTTATCCTCG CTGGCAACCT CCTCAGCCAC AGCACCCAGA GCAGGGATTC TATCAATAAG 
GCCAAATACC TCACCAAGAA AACCCAGGCA GCCAGCGTGG AGGCTGTTAA GATGCTGGAT 
GAGATCCTCC TGCAGCTGAG CGCCTCAGTG CCCGTGGACG TGATGCCAGG CGAGTTTGAT 
CCCACCAATT ACACGCTCCC CCAGCAGCCC CTCCACCCCT GCATGTTCCC GCTGGCCACT 
GCCTACTCCA CGCTCCAGCT GGTCACCAAC CCCTACCAGG CCACCATTGA TGGAGTCAGA 
TTTTTGGGGA CATCAGGACA GAACGTGAGT GACATTTTCC GATACAGCAG CATGGAGGAT 
CACTTGGAGA TCCTGGAGTG GACCCTGCGG GTCCGTCACA TCAGCCCCAC AGCCCCTGAC 
ACTCTAGGTT GTTACCCCTT CTACAAAACT GACCCGTTCA TCTTCCCAGA GTGCCCGCAT 
GTCTACTTTT GTGGCAACAC CCCCAGCTTT GGCTCCAAAA TCATCCGAGG TCCTGAGGAC 
CAGACAGTGC TGTTGGTGAC TGTCCCTGAC TTCAGTGCCA CGCAGACCGC CTGCCTTGTG 
AACCTGCGCA GCCTGGCCTG CCAGCCCATC AGCTTCTCGG GCTTCGGGGC AGAGGACGAT 
GACCTGGGAG GCCTGGGGCT GGGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024302570.1
CDS	185..1594
Translation	MFSEQAAQRAHTLLSPPSANNATFARVPVATYTNSSQPFRLGERSFSRQYAHIYATRLIQMRPFLENRAQQHWGSGVGVKKLCELQPEEKCCVVGTLFKAMPLQPSILREVSEEHNLLPQPPRSKYIHPDDELVLEDELQRIKLKGTIDVSKLVTGTVLAVFGSVRDDGKFLVEDYCFADLAPQKPAPPLDTDRFVLLVSGLGLGGGGGESLLGTQLLVDVVTGQLGDEGEQCSAAHVSRVILAGNLLSHSTQSRDSINKAKYLTKKTQAASVEAVKMLDEILLQLSASVPVDVMPGEFDPTNYTLPQQPLHPCMFPLATAYSTLQLVTNPYQATIDGVRFLGTSGQNVSDIFRYSSMEDHLEILEWTLRVRHISPTAPDTLGCYPFYKTDPFIFPECPHVYFCGNTPSFGSKIIRGPEDQTVLLVTVPDFSATQTACLVNLRSLACQPISFSGFGAEDDDLGGLGLGP

Target ORF information:

RefSeq Version	XM_024446802.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase delta 2, accessory subunit (POLD2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024446802.1

ORF Insert Sequence:

ATGTTTTCTG AGCAGGCTGC CCAGAGGGCC CACACTCTAC TGTCCCCACC ATCAGCCAAC 
AATGCCACCT TTGCCCGGGT GCCAGTGGCA ACCTACACCA ACTCCTCACA ACCCTTCCGG 
CTAGGAGAGC GCAGCTTTAG CCGGCAGTAT GCCCACATTT ATGCCACCCG CCTCATCCAA 
ATGAGACCCT TCCTGGAGAA CCGGGCCCAG CAGCACTGGG GCAGTGGAGT GGGAGTGAAG 
AAGCTGTGTG AACTGCAGCC TGAGGAGAAG TGCTGTGTGG TGGGCACTCT GTTCAAGGCC 
ATGCCGCTGC AGCCCTCCAT CCTGCGGGAG GTCAGCGAGG AGCACAACCT GCTCCCCCAG 
CCTCCTCGGA GTAAATACAT ACACCCAGAT GACGAGCTGG TCTTGGAAGA TGAACTGCAG 
CGTATCAAAC TAAAAGGCAC CATTGACGTG TCAAAGCTGG TTACGGGGAC TGTCCTGGCT 
GTGTTTGGCT CCGTGAGAGA CGACGGGAAG TTTCTGGTGG AGGACTATTG CTTTGCTGAC 
CTTGCTCCCC AGAAGCCCGC ACCCCCACTT GACACAGATA GGTTTGTGCT ACTGGTGTCC 
GGCCTGGGCC TGGGTGGCGG TGGAGGCGAG AGCCTGCTGG GCACCCAGCT GCTGGTGGAT 
GTGGTGACGG GGCAGCTTGG GGACGAAGGG GAGCAGTGCA GCGCCGCCCA CGTCTCCCGG 
GTTATCCTCG CTGGCAACCT CCTCAGCCAC AGCACCCAGA GCAGGGATTC TATCAATAAG 
GCCAAATACC TCACCAAGAA AACCCAGGCA GCCAGCGTGG AGGCTGTTAA GATGCTGGAT 
GAGATCCTCC TGCAGCTGAG CGCCTCAGTG CCCGTGGACG TGATGCCAGG CGAGTTTGAT 
CCCACCAATT ACACGCTCCC CCAGCAGCCC CTCCACCCCT GCATGTTCCC GCTGGCCACT 
GCCTACTCCA CGCTCCAGCT GGTCACCAAC CCCTACCAGG CCACCATTGA TGGAGTCAGA 
TTTTTGGGGA CATCAGGACA GAACGTGAGT GACATTTTCC GATACAGCAG CATGGAGGAT 
CACTTGGAGA TCCTGGAGTG GACCCTGCGG GTCCGTCACA TCAGCCCCAC AGCCCCTGAC 
ACTCTAGGTT GTTACCCCTT CTACAAAACT GACCCGTTCA TCTTCCCAGA GTGCCCGCAT 
GTCTACTTTT GTGGCAACAC CCCCAGCTTT GGCTCCAAAA TCATCCGAGG TCCTGAGGAC 
CAGACAGTGC TGTTGGTGAC TGTCCCTGAC TTCAGTGCCA CGCAGACCGC CTGCCTTGTG 
AACCTGCGCA GCCTGGCCTG CCAGCCCATC AGCTTCTCGG GCTTCGGGGC AGAGGACGAT 
GACCTGGGAG GCCTGGGGCT GGGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLD2 ( NM_006230.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006221.2 Homo sapiens DNA polymerase delta 2, accessory subunit (POLD2), transcript variant 2, mRNA.

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CloneID	OHu05462
Clone ID Related Accession (Same CDS sequence)	NM_006230.3
Accession Version	NM_006230.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	DNA polymerase delta subunit 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP224472.1, BC000459.2 and AW732014.1. On Mar 3, 2012 this sequence version replaced NM_006230.2. Transcript Variant: This variant (2) uses an alternate splice site in the 5' region and initiates translation at an upstream start codon, compared to variant 1. The encoded isoform (2) has a longer N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.412273.1, SRR1163657.234211.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGGG CAGGCGCGCG GGGATTGGCG GGATGCGGCG CGCCGCGCGT GAACCTCCTT 
GGCCTGGGCG AAGCTGTGTG GACCAAGCAA GTCAGGAGTG TGGCCATGTT TTCTGAGCAG 
GCTGCCCAGA GGGCCCACAC TCTACTGTCC CCACCATCAG CCAACAATGC CACCTTTGCC 
CGGGTGCCAG TGGCAACCTA CACCAACTCC TCACAACCCT TCCGGCTAGG AGAGCGCAGC 
TTTAGCCGGC AGTATGCCCA CATTTATGCC ACCCGCCTCA TCCAAATGAG ACCCTTCCTG 
GAGAACCGGG CCCAGCAGCA CTGGGGCAGT GGAGTGGGAG TGAAGAAGCT GTGTGAACTG 
CAGCCTGAGG AGAAGTGCTG TGTGGTGGGC ACTCTGTTCA AGGCCATGCC GCTGCAGCCC 
TCCATCCTGC GGGAGGTCAG CGAGGAGCAC AACCTGCTCC CCCAGCCTCC TCGGAGTAAA 
TACATACACC CAGATGACGA GCTGGTCTTG GAAGATGAAC TGCAGCGTAT CAAACTAAAA 
GGCACCATTG ACGTGTCAAA GCTGGTTACG GGGACTGTCC TGGCTGTGTT TGGCTCCGTG 
AGAGACGACG GGAAGTTTCT GGTGGAGGAC TATTGCTTTG CTGACCTTGC TCCCCAGAAG 
CCCGCACCCC CACTTGACAC AGATAGGTTT GTGCTACTGG TGTCCGGCCT GGGCCTGGGT 
GGCGGTGGAG GCGAGAGCCT GCTGGGCACC CAGCTGCTGG TGGATGTGGT GACGGGGCAG 
CTTGGGGACG AAGGGGAGCA GTGCAGCGCC GCCCACGTCT CCCGGGTTAT CCTCGCTGGC 
AACCTCCTCA GCCACAGCAC CCAGAGCAGG GATTCTATCA ATAAGGCCAA ATACCTCACC 
AAGAAAACCC AGGCAGCCAG CGTGGAGGCT GTTAAGATGC TGGATGAGAT CCTCCTGCAG 
CTGAGCGCCT CAGTGCCCGT GGACGTGATG CCAGGCGAGT TTGATCCCAC CAATTACACG 
CTCCCCCAGC AGCCCCTCCA CCCCTGCATG TTCCCGCTGG CCACTGCCTA CTCCACGCTC 
CAGCTGGTCA CCAACCCCTA CCAGGCCACC ATTGATGGAG TCAGATTTTT GGGGACATCA 
GGACAGAACG TGAGTGACAT TTTCCGATAC AGCAGCATGG AGGATCACTT GGAGATCCTG 
GAGTGGACCC TGCGGGTCCG TCACATCAGC CCCACAGCCC CTGACACTCT AGGTTGTTAC 
CCCTTCTACA AAACTGACCC GTTCATCTTC CCAGAGTGCC CGCATGTCTA CTTTTGTGGC 
AACACCCCCA GCTTTGGCTC CAAAATCATC CGAGGTCCTG AGGACCAGAC AGTGCTGTTG 
GTGACTGTCC CTGACTTCAG TGCCACGCAG ACCGCCTGCC TTGTGAACCT GCGCAGCCTG 
GCCTGCCAGC CCATCAGCTT CTCGGGCTTC GGGGCAGAGG ACGATGACCT GGGAGGCCTG 
GGGCTGGGCC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006221.2
CDS	12..1526
Translation	MGGAGARGLAGCGAPRVNLLGLGEAVWTKQVRSVAMFSEQAAQRAHTLLSPPSANNATFARVPVATYTNSSQPFRLGERSFSRQYAHIYATRLIQMRPFLENRAQQHWGSGVGVKKLCELQPEEKCCVVGTLFKAMPLQPSILREVSEEHNLLPQPPRSKYIHPDDELVLEDELQRIKLKGTIDVSKLVTGTVLAVFGSVRDDGKFLVEDYCFADLAPQKPAPPLDTDRFVLLVSGLGLGGGGGESLLGTQLLVDVVTGQLGDEGEQCSAAHVSRVILAGNLLSHSTQSRDSINKAKYLTKKTQAASVEAVKMLDEILLQLSASVPVDVMPGEFDPTNYTLPQQPLHPCMFPLATAYSTLQLVTNPYQATIDGVRFLGTSGQNVSDIFRYSSMEDHLEILEWTLRVRHISPTAPDTLGCYPFYKTDPFIFPECPHVYFCGNTPSFGSKIIRGPEDQTVLLVTVPDFSATQTACLVNLRSLACQPISFSGFGAEDDDLGGLGLGP

Target ORF information:

RefSeq Version	NM_006230.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase delta 2, accessory subunit (POLD2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006230.3

ORF Insert Sequence:

ATGGGCGGGG CAGGCGCGCG GGGATTGGCG GGATGCGGCG CGCCGCGCGT GAACCTCCTT 
GGCCTGGGCG AAGCTGTGTG GACCAAGCAA GTCAGGAGTG TGGCCATGTT TTCTGAGCAG 
GCTGCCCAGA GGGCCCACAC TCTACTGTCC CCACCATCAG CCAACAATGC CACCTTTGCC 
CGGGTGCCAG TGGCAACCTA CACCAACTCC TCACAACCCT TCCGGCTAGG AGAGCGCAGC 
TTTAGCCGGC AGTATGCCCA CATTTATGCC ACCCGCCTCA TCCAAATGAG ACCCTTCCTG 
GAGAACCGGG CCCAGCAGCA CTGGGGCAGT GGAGTGGGAG TGAAGAAGCT GTGTGAACTG 
CAGCCTGAGG AGAAGTGCTG TGTGGTGGGC ACTCTGTTCA AGGCCATGCC GCTGCAGCCC 
TCCATCCTGC GGGAGGTCAG CGAGGAGCAC AACCTGCTCC CCCAGCCTCC TCGGAGTAAA 
TACATACACC CAGATGACGA GCTGGTCTTG GAAGATGAAC TGCAGCGTAT CAAACTAAAA 
GGCACCATTG ACGTGTCAAA GCTGGTTACG GGGACTGTCC TGGCTGTGTT TGGCTCCGTG 
AGAGACGACG GGAAGTTTCT GGTGGAGGAC TATTGCTTTG CTGACCTTGC TCCCCAGAAG 
CCCGCACCCC CACTTGACAC AGATAGGTTT GTGCTACTGG TGTCCGGCCT GGGCCTGGGT 
GGCGGTGGAG GCGAGAGCCT GCTGGGCACC CAGCTGCTGG TGGATGTGGT GACGGGGCAG 
CTTGGGGACG AAGGGGAGCA GTGCAGCGCC GCCCACGTCT CCCGGGTTAT CCTCGCTGGC 
AACCTCCTCA GCCACAGCAC CCAGAGCAGG GATTCTATCA ATAAGGCCAA ATACCTCACC 
AAGAAAACCC AGGCAGCCAG CGTGGAGGCT GTTAAGATGC TGGATGAGAT CCTCCTGCAG 
CTGAGCGCCT CAGTGCCCGT GGACGTGATG CCAGGCGAGT TTGATCCCAC CAATTACACG 
CTCCCCCAGC AGCCCCTCCA CCCCTGCATG TTCCCGCTGG CCACTGCCTA CTCCACGCTC 
CAGCTGGTCA CCAACCCCTA CCAGGCCACC ATTGATGGAG TCAGATTTTT GGGGACATCA 
GGACAGAACG TGAGTGACAT TTTCCGATAC AGCAGCATGG AGGATCACTT GGAGATCCTG 
GAGTGGACCC TGCGGGTCCG TCACATCAGC CCCACAGCCC CTGACACTCT AGGTTGTTAC 
CCCTTCTACA AAACTGACCC GTTCATCTTC CCAGAGTGCC CGCATGTCTA CTTTTGTGGC 
AACACCCCCA GCTTTGGCTC CAAAATCATC CGAGGTCCTG AGGACCAGAC AGTGCTGTTG 
GTGACTGTCC CTGACTTCAG TGCCACGCAG ACCGCCTGCC TTGTGAACCT GCGCAGCCTG 
GCCTGCCAGC CCATCAGCTT CTCGGGCTTC GGGGCAGAGG ACGATGACCT GGGAGGCCTG 
GGGCTGGGCC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLD2 ( XM_011515431.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513733.1 Homo sapiens polymerase (DNA) delta 2, accessory subunit (POLD2), transcript variant X1, mRNA.

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CloneID	OHu59807
Clone ID Related Accession (Same CDS sequence)	XM_011515431.1
Accession Version	XM_011515431.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DNA polymerase delta subunit 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCGCC TGGTGACGGA CGGGGCGAGG AGGGCAGCGA TTGTGAACCT CCTTGGCCTG 
GGCGAAGCTG TGTGGACCAA GCAAGTCAGG AGTGTGGCCA TGTTTTCTGA GCAGGCTGCC 
CAGAGGGCCC ACACTCTACT GTCCCCACCA TCAGCCAACA ATGCCACCTT TGCCCGGGTG 
CCAGTGGCAA CCTACACCAA CTCCTCACAA CCCTTCCGGC TAGGAGAGCG CAGCTTTAGC 
CGGCAGTATG CCCACATTTA TGCCACCCGC CTCATCCAAA TGAGACCCTT CCTGGAGAAC 
CGGGCCCAGC AGCACTGGGG CAGTGGAGTG GGAGTGAAGA AGCTGTGTGA ACTGCAGCCT 
GAGGAGAAGT GCTGTGTGGT GGGCACTCTG TTCAAGGCCA TGCCGCTGCA GCCCTCCATC 
CTGCGGGAGG TCAGCGAGGA GCACAACCTG CTCCCCCAGC CTCCTCGGAG TAAATACATA 
CACCCAGATG ACGAGCTGGT CTTGGAAGAT GAACTGCAGC GTATCAAACT AAAAGGCACC 
ATTGACGTGT CAAAGCTGGT TACGGGGACT GTCCTGGCTG TGTTTGGCTC CGTGAGAGAC 
GACGGGAAGT TTCTGGTGGA GGACTATTGC TTTGCTGACC TTGCTCCCCA GAAGCCCGCA 
CCCCCACTTG ACACAGATAG GTTTGTGCTA CTGGTGTCCG GCCTGGGCCT GGGTGGCGGT 
GGAGGCGAGA GCCTGCTGGG CACCCAGCTG CTGGTGGATG TGGTGACGGG GCAGCTTGGG 
GACGAAGGGG AGCAGTGCAG CGCCGCCCAC GTCTCCCGGG TTATCCTCGC TGGCAACCTC 
CTCAGCCACA GCACCCAGAG CAGGGATTCT ATCAATAAGG CCAAATACCT CACCAAGAAA 
ACCCAGGCAG CCAGCGTGGA GGCTGTTAAG ATGCTGGATG AGATCCTCCT GCAGCTGAGC 
GCCTCAGTGC CCGTGGACGT GATGCCAGGC GAGTTTGATC CCACCAATTA CACGCTCCCC 
CAGCAGCCCC TCCACCCCTG CATGTTCCCG CTGGCCACTG CCTACTCCAC GCTCCAGCTG 
GTCACCAACC CCTACCAGGC CACCATTGAT GGAGTCAGAT TTTTGGGGAC ATCAGGACAG 
AACGTGAGTG ACATTTTCCG ATACAGCAGC ATGGAGGATC ACTTGGAGAT CCTGGAGTGG 
ACCCTGCGGG TCCGTCACAT CAGCCCCACA GCCCCTGACA CTCTAGGTTG TTACCCCTTC 
TACAAAACTG ACCCGTTCAT CTTCCCAGAG TGCCCGCATG TCTACTTTTG TGGCAACACC 
CCCAGCTTTG GCTCCAAAAT CATCCGAGGT CCTGAGGACC AGACAGTGCT GTTGGTGACT 
GTCCCTGACT TCAGTGCCAC GCAGACCGCC TGCCTTGTGA ACCTGCGCAG CCTGGCCTGC 
CAGCCCATCA GCTTCTCGGG CTTCGGGGCA GAGGACGATG ACCTGGGAGG CCTGGGGCTG 
GGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513733.1
CDS	14..1522
Translation	MRRLVTDGARRAAIVNLLGLGEAVWTKQVRSVAMFSEQAAQRAHTLLSPPSANNATFARVPVATYTNSSQPFRLGERSFSRQYAHIYATRLIQMRPFLENRAQQHWGSGVGVKKLCELQPEEKCCVVGTLFKAMPLQPSILREVSEEHNLLPQPPRSKYIHPDDELVLEDELQRIKLKGTIDVSKLVTGTVLAVFGSVRDDGKFLVEDYCFADLAPQKPAPPLDTDRFVLLVSGLGLGGGGGESLLGTQLLVDVVTGQLGDEGEQCSAAHVSRVILAGNLLSHSTQSRDSINKAKYLTKKTQAASVEAVKMLDEILLQLSASVPVDVMPGEFDPTNYTLPQQPLHPCMFPLATAYSTLQLVTNPYQATIDGVRFLGTSGQNVSDIFRYSSMEDHLEILEWTLRVRHISPTAPDTLGCYPFYKTDPFIFPECPHVYFCGNTPSFGSKIIRGPEDQTVLLVTVPDFSATQTACLVNLRSLACQPISFSGFGAEDDDLGGLGLGP

Target ORF information:

RefSeq Version	XM_011515431.1
Organism	Homo sapiens(human)
Definition	Homo sapiens polymerase (DNA) delta 2, accessory subunit (POLD2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515431.1

ORF Insert Sequence:

ATGAGGCGCC TGGTGACGGA CGGGGCGAGG AGGGCAGCGA TTGTGAACCT CCTTGGCCTG 
GGCGAAGCTG TGTGGACCAA GCAAGTCAGG AGTGTGGCCA TGTTTTCTGA GCAGGCTGCC 
CAGAGGGCCC ACACTCTACT GTCCCCACCA TCAGCCAACA ATGCCACCTT TGCCCGGGTG 
CCAGTGGCAA CCTACACCAA CTCCTCACAA CCCTTCCGGC TAGGAGAGCG CAGCTTTAGC 
CGGCAGTATG CCCACATTTA TGCCACCCGC CTCATCCAAA TGAGACCCTT CCTGGAGAAC 
CGGGCCCAGC AGCACTGGGG CAGTGGAGTG GGAGTGAAGA AGCTGTGTGA ACTGCAGCCT 
GAGGAGAAGT GCTGTGTGGT GGGCACTCTG TTCAAGGCCA TGCCGCTGCA GCCCTCCATC 
CTGCGGGAGG TCAGCGAGGA GCACAACCTG CTCCCCCAGC CTCCTCGGAG TAAATACATA 
CACCCAGATG ACGAGCTGGT CTTGGAAGAT GAACTGCAGC GTATCAAACT AAAAGGCACC 
ATTGACGTGT CAAAGCTGGT TACGGGGACT GTCCTGGCTG TGTTTGGCTC CGTGAGAGAC 
GACGGGAAGT TTCTGGTGGA GGACTATTGC TTTGCTGACC TTGCTCCCCA GAAGCCCGCA 
CCCCCACTTG ACACAGATAG GTTTGTGCTA CTGGTGTCCG GCCTGGGCCT GGGTGGCGGT 
GGAGGCGAGA GCCTGCTGGG CACCCAGCTG CTGGTGGATG TGGTGACGGG GCAGCTTGGG 
GACGAAGGGG AGCAGTGCAG CGCCGCCCAC GTCTCCCGGG TTATCCTCGC TGGCAACCTC 
CTCAGCCACA GCACCCAGAG CAGGGATTCT ATCAATAAGG CCAAATACCT CACCAAGAAA 
ACCCAGGCAG CCAGCGTGGA GGCTGTTAAG ATGCTGGATG AGATCCTCCT GCAGCTGAGC 
GCCTCAGTGC CCGTGGACGT GATGCCAGGC GAGTTTGATC CCACCAATTA CACGCTCCCC 
CAGCAGCCCC TCCACCCCTG CATGTTCCCG CTGGCCACTG CCTACTCCAC GCTCCAGCTG 
GTCACCAACC CCTACCAGGC CACCATTGAT GGAGTCAGAT TTTTGGGGAC ATCAGGACAG 
AACGTGAGTG ACATTTTCCG ATACAGCAGC ATGGAGGATC ACTTGGAGAT CCTGGAGTGG 
ACCCTGCGGG TCCGTCACAT CAGCCCCACA GCCCCTGACA CTCTAGGTTG TTACCCCTTC 
TACAAAACTG ACCCGTTCAT CTTCCCAGAG TGCCCGCATG TCTACTTTTG TGGCAACACC 
CCCAGCTTTG GCTCCAAAAT CATCCGAGGT CCTGAGGACC AGACAGTGCT GTTGGTGACT 
GTCCCTGACT TCAGTGCCAC GCAGACCGCC TGCCTTGTGA ACCTGCGCAG CCTGGCCTGC 
CAGCCCATCA GCTTCTCGGG CTTCGGGGCA GAGGACGATG ACCTGGGAGG CCTGGGGCTG 
GGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human DNA polymerase delta double-mutant D316A;E318A interferes with DNA mismatch repair in vitro.
Nucleic acids research45(16)9427-9440(2017 Sep)
Liu D,Frederiksen JH,Liberti SE,L?tzen A,Keijzers G,Pena-Diaz J,Rasmussen LJ

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M

DNA Polymerases as targets for gene therapy of hepatocellular carcinoma.
BMC cancer15325(2015 Apr)
Liu H,Wei Q,Wang J,Huang X,Li C,Zheng Q,Cao J,Jia Z

POLD2 and KSP37 (FGFBP2) correlate strongly with histology, stage and outcome in ovarian carcinomas.
PloS one5(11)e13837(2010 Nov)
Elgaaen BV,Haug KB,Wang J,Olstad OK,Fortunati D,Onsrud M,Staff AC,Sauer T,Gautvik KM

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POLD2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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