CYCS cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CYCS
Entrez Gene ID	54205
Full Name	cytochrome c, somatic
Synonyms	CYC,HCS,THC4
General protein information	Preferred Names cytochrome c, somatic Names cytochrome c
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7p15.3
Summary	This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010].
Disorder MIM:	123970
Disorder Html:	Thrombocytopenia 4, 612004 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_018947.5	NP_061820.1	cytochrome c
NM_018947.6	NP_061820.1	cytochrome c

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CYCS	NP_001039526.1
Rattus norvegicus (Norway rat)	Cycs	NP_036971.1
Drosophila melanogaster (fruit fly)	Cyt-c-p	NP_477176.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009537	XP_310154.1
Eremothecium gossypii	AGOS_AFR360W	NP_985907.1
Rattus norvegicus (Norway rat)	LOC100363502	XP_003751847.1
Gallus gallus (chicken)	CYCS	NP_001072946.1
Homo sapiens (human)	CYCS	NP_061820.1
Caenorhabditis elegans (roundworm)	cyc-2.2	NP_506156.1
Kluyveromyces lactis	KLLA0F16929g	XP_455841.1
Xenopus tropicalis (tropical clawed frog)	cycs	NP_988895.1
	LOC694977	XP_001085135.1
Canis lupus familiaris (dog)	CYCS	NP_001183974.1
Mus musculus (house mouse)	Cycs	NP_031834.1
Caenorhabditis elegans (roundworm)	cyc-2.1	NP_500629.1
Saccharomyces cerevisiae (baker's yeast)	CYC1	NP_012582.1

Gene Ontology
Bibliography

Related articles in PubMed

Cytochrome c is important in apoptosis of labial glands in primary Sjogren's syndrome.
Kang Y, Sun Y, Zhang Y, Wang Z
Molecular medicine reports17(1)1993-1997(2018 Jan)

Heightened Dynamics of the Oxidized Y48H Variant of Human Cytochrome c Increases Its Peroxidatic Activity.
Deacon OM, Karsisiotis AI, Moreno-Chicano T, Hough MA, Macdonald C, Blumenschein TMA, Wilson MT, Moore GR, Worrall JAR
Biochemistry56(46)6111-6124(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The naturally occurring Y48H variant of cytochrome c in its oxidized heme state is more peroxidatic than either the Wild Type protein or the G41S variant that is also implicated in thrombocytopenia.
Title: Heightened Dynamics of the Oxidized Y48H Variant of Human Cytochrome c Increases Its Peroxidatic Activity.

Data suggest that the stronger effect of K72A mutation on the peroxidase activity of human versus yeast cytochrome c results from relief of steric interactions between side chains at positions 72 and 81 (Ile in human vs Ala in yeast), which suppresses the dynamics of omega-loop D necessary for the intrinsic peroxidase activity of cytochrome c.
Title: Effect of a K72A Mutation on the Structure, Stability, Dynamics, and Peroxidase Activity of Human Cytochrome c.

Data suggest that although HCCS mediates heme attachment to N-terminal cysteine in heme-attachment site (CXXXH) of cytochrome C variants, up to 50% of cytochrome C produced is modified in an oxygen-dependent manner, resulting in a mixed population of cytochrome c. [HCCS = holocytochrome c synthase]
Title: Biosynthesis of Single Thioether c-Type Cytochromes Provides Insight into Mechanisms Intrinsic to Holocytochrome c Synthase (HCCS).

ROCK activation phosphorylated Rac1b at Ser71 and increased reactive oxygen species (ROS) levels by facilitating the interaction between Rac1b and cytochrome c. Conversely, ROCK inactivation abolished their interaction, concomitant with ROS reduction.
Title: Chemical screening identifies ROCK as a target for recovering mitochondrial function in Hutchinson-Gilford progeria syndrome.

These findings establish a framework for understanding the molecular basis of cytochrome c-mediated blocking of SET/TAF-Ibeta.
Title: Structural basis for inhibition of the histone chaperone activity of SET/TAF-I? by cytochrome c.

The following CYCS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CYCS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22841	NM_018947.6 Latest version!	Homo sapiens cytochrome c, somatic (CYCS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu22841	NM_018947.5	Homo sapiens cytochrome c, somatic (CYCS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CYCS ( NM_018947.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_061820.1 Homo sapiens cytochrome c, somatic (CYCS), mRNA.

Price & Availability↑

CloneID	OHu22841
Clone ID Related Accession (Same CDS sequence)	NM_018947.5 , NM_018947.6
Accession Version	NM_018947.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	cytochrome c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004129.2, DB447825.1, BC024216.1, AC007487.2 and AI365318.1. This sequence is a reference standard in the RefSeqGene project. On Jul 20, 2010 this sequence version replaced NM_018947.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DB447825.1, SRR1803617.270352.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGTGATG TTGAGAAAGG CAAGAAGATT TTTATTATGA AGTGTTCCCA GTGCCACACC 
GTTGAAAAGG GAGGCAAGCA CAAGACTGGG CCAAATCTCC ATGGTCTCTT TGGGCGGAAG 
ACAGGTCAGG CCCCTGGATA CTCTTACACA GCCGCCAATA AGAACAAAGG CATCATCTGG 
GGAGAGGATA CACTGATGGA GTATTTGGAG AATCCCAAGA AGTACATCCC TGGAACAAAA 
ATGATCTTTG TCGGCATTAA GAAGAAGGAA GAAAGGGCAG ACTTAATAGC TTATCTCAAA 
AAAGCTACTA ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061820.1
CDS	171..488
Translation	MGDVEKGKKIFIMKCSQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGYSYTAANKNKGIIWGEDTLMEYLENPKKYIPGTKMIFVGIKKKEERADLIAYLKKATNE

Target ORF information:

RefSeq Version	NM_018947.5
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome c, somatic (CYCS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018947.5

ORF Insert Sequence:

ATGGGTGATG TTGAGAAAGG CAAGAAGATT TTTATTATGA AGTGTTCCCA GTGCCACACC 
GTTGAAAAGG GAGGCAAGCA CAAGACTGGG CCAAATCTCC ATGGTCTCTT TGGGCGGAAG 
ACAGGTCAGG CCCCTGGATA CTCTTACACA GCCGCCAATA AGAACAAAGG CATCATCTGG 
GGAGAGGATA CACTGATGGA GTATTTGGAG AATCCCAAGA AGTACATCCC TGGAACAAAA 
ATGATCTTTG TCGGCATTAA GAAGAAGGAA GAAAGGGCAG ACTTAATAGC TTATCTCAAA 
AAAGCTACTA ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYCS ( NM_018947.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_061820.1 Homo sapiens cytochrome c, somatic (CYCS), mRNA.

Price & Availability↑

CloneID	OHu22841
Clone ID Related Accession (Same CDS sequence)	NM_018947.5 , NM_018947.6
Accession Version	NM_018947.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Homo sapiens(human)
Product	cytochrome c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB447825.1, BC024216.1, AC007487.2 and AI365318.1. On Aug 31, 2019 this sequence version replaced NM_018947.5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL713681.1, BC009582.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGTGATG TTGAGAAAGG CAAGAAGATT TTTATTATGA AGTGTTCCCA GTGCCACACC 
GTTGAAAAGG GAGGCAAGCA CAAGACTGGG CCAAATCTCC ATGGTCTCTT TGGGCGGAAG 
ACAGGTCAGG CCCCTGGATA CTCTTACACA GCCGCCAATA AGAACAAAGG CATCATCTGG 
GGAGAGGATA CACTGATGGA GTATTTGGAG AATCCCAAGA AGTACATCCC TGGAACAAAA 
ATGATCTTTG TCGGCATTAA GAAGAAGGAA GAAAGGGCAG ACTTAATAGC TTATCTCAAA 
AAAGCTACTA ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061820.1
CDS	70..387
Translation	MGDVEKGKKIFIMKCSQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGYSYTAANKNKGIIWGEDTLMEYLENPKKYIPGTKMIFVGIKKKEERADLIAYLKKATNE

Target ORF information:

RefSeq Version	NM_018947.6
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome c, somatic (CYCS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018947.6

ORF Insert Sequence:

ATGGGTGATG TTGAGAAAGG CAAGAAGATT TTTATTATGA AGTGTTCCCA GTGCCACACC 
GTTGAAAAGG GAGGCAAGCA CAAGACTGGG CCAAATCTCC ATGGTCTCTT TGGGCGGAAG 
ACAGGTCAGG CCCCTGGATA CTCTTACACA GCCGCCAATA AGAACAAAGG CATCATCTGG 
GGAGAGGATA CACTGATGGA GTATTTGGAG AATCCCAAGA AGTACATCCC TGGAACAAAA 
ATGATCTTTG TCGGCATTAA GAAGAAGGAA GAAAGGGCAG ACTTAATAGC TTATCTCAAA 
AAAGCTACTA ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cytochrome c is important in apoptosis of labial glands in primary Sjogren's syndrome.
Molecular medicine reports17(1)1993-1997(2018 Jan)
Kang Y,Sun Y,Zhang Y,Wang Z

Heightened Dynamics of the Oxidized Y48H Variant of Human Cytochrome c Increases Its Peroxidatic Activity.
Biochemistry56(46)6111-6124(2017 Nov)
Deacon OM,Karsisiotis AI,Moreno-Chicano T,Hough MA,Macdonald C,Blumenschein TMA,Wilson MT,Moore GR,Worrall JAR

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CYCS cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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