PNMT cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PNMT
Entrez Gene ID	5409
Full Name	phenylethanolamine N-methyltransferase
Synonyms	PENT,PNMTase
General protein information	Preferred Names phenylethanolamine N-methyltransferase Names phenylethanolamine N-methyltransferase noradrenaline N-methyltransferase phenylethanolamine N-methylase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q12
Summary	The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012].
Disorder MIM:	171190

mRNA and Protein(s)

mRNA	Protein	Name
XM_011524909.2	XP_011523211.1	phenylethanolamine N-methyltransferase isoform X1
NM_002686.4	NP_002677.1	phenylethanolamine N-methyltransferase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PNMT	XP_511459.2
Canis lupus familiaris (dog)	PNMT	XP_548143.3
Xenopus tropicalis (tropical clawed frog)	pnmt	XP_002940421.1
Homo sapiens (human)	PNMT	NP_002677.1
	PNMT	XP_001089850.2
Mus musculus (house mouse)	Pnmt	NP_032916.1
Gallus gallus (chicken)	PNMT	XP_003642859.1
Rattus norvegicus (Norway rat)	Pnmt	NP_113714.1
Bos taurus (cattle)	PNMT	NP_803471.2
Danio rerio (zebrafish)	LOC100332609	XP_002666387.2

Gene Ontology
Bibliography

Related articles in PubMed

Phenylethanolamine N-methyltransferase downregulation is associated with malignant pheochromocytoma/paraganglioma.
Lee SE, Oh E, Lee B, Kim YJ, Oh DY, Jung K, Choi JS, Kim J, Kim SJ, Yang JW, An J, Oh YL, Choi YL
Oncotarget7(17)24141-53(2016 Apr)

Kinetic and pH studies on human phenylethanolamine N-methyltransferase.
Wu Q, McLeish MJ
Archives of biochemistry and biophysics539(1)1-8(2013 Nov)

Phenylethanolamine N-methyltransferase gene promoter haplotypes and risk of essential hypertension.
Huang C, Zhang S, Hu K, Ma Q, Yang T
American journal of hypertension24(11)1222-6(2011 Nov)

A single human cell expresses all messenger ribonucleic acids: the arrow of time in a cell.
Kimoto Y
Molecular & general genetics : MGG258(3)233-9(1998 May)

Structure of human phenylethanolamine n-methyltransferase gene: existence of two types of mRNA with different transcription initiation sites.
Sasaoka T, Kaneda N, Kurosawa Y, Fujita K, Nagatsu T
Neurochemistry international15(4)555-65(1989)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PNMT underexpression is associated with malignant pheochromocytoma and paraganglioma.
Title: Phenylethanolamine N-methyltransferase downregulation is associated with malignant pheochromocytoma/paraganglioma.

initial velocity studies on human PNMT in the absence and presence of product and dead end inhibitors.
Title: Kinetic and pH studies on human phenylethanolamine N-methyltransferase.

The 2-SNP AA haplotype in the PNMT promoter is associated with decreased risk of essential hypertension in Han Chinese.
Title: Phenylethanolamine N-methyltransferase gene promoter haplotypes and risk of essential hypertension.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Association study of 182 candidate genes in anorexia nervosa.

The following PNMT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PNMT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu00619	NM_002686.4 Latest version!	Homo sapiens phenylethanolamine N-methyltransferase (PNMT), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu59802	XM_011524909.2 Latest version!	Homo sapiens phenylethanolamine N-methyltransferase (PNMT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PNMT ( NM_002686.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002677.1 Homo sapiens phenylethanolamine N-methyltransferase (PNMT), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu00619
Clone ID Related Accession (Same CDS sequence)	NM_002686.4
Accession Version	NM_002686.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	phenylethanolamine N-methyltransferase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC037246.1. This sequence is a reference standard in the RefSeqGene project. On Nov 27, 2012 this sequence version replaced NM_002686.3. Transcript Variant: This variant (1) encodes a functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC037246.1, J03727.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000269582.3/ ENSP00000269582.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCGGCG CAGACCGTAG CCCCAATGCG GGCGCAGCCC CTGACTCGGC CCCGGGCCAG 
GCGGCGGTGG CTTCGGCCTA CCAGCGCTTC GAGCCGCGCG CCTACCTCCG CAACAACTAC 
GCGCCCCCTC GCGGGGACCT GTGCAACCCG AACGGCGTCG GGCCGTGGAA GCTGCGCTGC 
TTGGCGCAGA CCTTCGCCAC CGGTGAAGTG TCCGGACGCA CCCTCATCGA CATTGGTTCA 
GGCCCCACCG TGTACCAGCT GCTCAGTGCC TGCAGCCACT TTGAGGACAT CACCATGACA 
GATTTCCTGG AGGTCAACCG CCAGGAGCTG GGGCGCTGGC TGCAGGAGGA GCCGGGGGCC 
TTCAACTGGA GCATGTACAG CCAACATGCC TGCCTCATTG AGGGCAAGGG GGAATGCTGG 
CAGGATAAGG AGCGCCAGCT GCGAGCCAGG GTGAAACGGG TCCTGCCCAT CGACGTGCAC 
CAGCCCCAGC CCCTGGGTGC TGGGAGCCCA GCTCCCCTGC CTGCTGACGC CCTGGTCTCT 
GCCTTCTGCT TGGAGGCTGT GAGCCCAGAT CTTGCCAGCT TTCAGCGGGC CCTGGACCAC 
ATCACCACGC TGCTGAGGCC TGGGGGGCAC CTCCTCCTCA TCGGGGCCCT GGAGGAGTCG 
TGGTACCTGG CTGGGGAGGC CAGGCTGACG GTGGTGCCAG TGTCTGAGGA GGAGGTGAGG 
GAGGCCCTGG TGCGTAGTGG CTACAAGGTC CGGGACCTCC GCACCTATAT CATGCCTGCC 
CACCTTCAGA CAGGCGTAGA TGATGTCAAG GGCGTCTTCT TCGCCTGGGC TCAGAAGGTT 
GGGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002677.1
CDS	24..872
Translation	MSGADRSPNAGAAPDSAPGQAAVASAYQRFEPRAYLRNNYAPPRGDLCNPNGVGPWKLRCLAQTFATGEVSGRTLIDIGSGPTVYQLLSACSHFEDITMTDFLEVNRQELGRWLQEEPGAFNWSMYSQHACLIEGKGECWQDKERQLRARVKRVLPIDVHQPQPLGAGSPAPLPADALVSAFCLEAVSPDLASFQRALDHITTLLRPGGHLLLIGALEESWYLAGEARLTVVPVSEEEVREALVRSGYKVRDLRTYIMPAHLQTGVDDVKGVFFAWAQKVGL

Target ORF information:

RefSeq Version	NM_002686.4
Organism	Homo sapiens(human)
Definition	Homo sapiens phenylethanolamine N-methyltransferase (PNMT), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002686.4

ORF Insert Sequence:

ATGAGCGGCG CAGACCGTAG CCCCAATGCG GGCGCAGCCC CTGACTCGGC CCCGGGCCAG 
GCGGCGGTGG CTTCGGCCTA CCAGCGCTTC GAGCCGCGCG CCTACCTCCG CAACAACTAC 
GCGCCCCCTC GCGGGGACCT GTGCAACCCG AACGGCGTCG GGCCGTGGAA GCTGCGCTGC 
TTGGCGCAGA CCTTCGCCAC CGGTGAAGTG TCCGGACGCA CCCTCATCGA CATTGGTTCA 
GGCCCCACCG TGTACCAGCT GCTCAGTGCC TGCAGCCACT TTGAGGACAT CACCATGACA 
GATTTCCTGG AGGTCAACCG CCAGGAGCTG GGGCGCTGGC TGCAGGAGGA GCCGGGGGCC 
TTCAACTGGA GCATGTACAG CCAACATGCC TGCCTCATTG AGGGCAAGGG GGAATGCTGG 
CAGGATAAGG AGCGCCAGCT GCGAGCCAGG GTGAAACGGG TCCTGCCCAT CGACGTGCAC 
CAGCCCCAGC CCCTGGGTGC TGGGAGCCCA GCTCCCCTGC CTGCTGACGC CCTGGTCTCT 
GCCTTCTGCT TGGAGGCTGT GAGCCCAGAT CTTGCCAGCT TTCAGCGGGC CCTGGACCAC 
ATCACCACGC TGCTGAGGCC TGGGGGGCAC CTCCTCCTCA TCGGGGCCCT GGAGGAGTCG 
TGGTACCTGG CTGGGGAGGC CAGGCTGACG GTGGTGCCAG TGTCTGAGGA GGAGGTGAGG 
GAGGCCCTGG TGCGTAGTGG CTACAAGGTC CGGGACCTCC GCACCTATAT CATGCCTGCC 
CACCTTCAGA CAGGCGTAGA TGATGTCAAG GGCGTCTTCT TCGCCTGGGC TCAGAAGGTT 
GGGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNMT ( XM_011524909.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523211.1 Homo sapiens phenylethanolamine N-methyltransferase (PNMT), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu59802
Clone ID Related Accession (Same CDS sequence)	XM_011524909.2
Accession Version	XM_011524909.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	phenylethanolamine N-methyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524909.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGATT TCCTGGAGGT CAACCGCCAG GAGCTGGGGC GCTGGCTGCA GGAGGAGCCG 
GGGGCCTTCA ACTGGAGCAT GTACAGCCAA CATGCCTGCC TCATTGAGGG CAAGGGGGAA 
TGCTGGCAGG ATAAGGAGCG CCAGCTGCGA GCCAGGGTGA AACGGGTCCT GCCCATCGAC 
GTGCACCAGC CCCAGCCCCT GGGTGCTGGG AGCCCAGCTC CCCTGCCTGC TGACGCCCTG 
GTCTCTGCCT TCTGCTTGGA GGCTGTGAGC CCAGATCTTG CCAGCTTTCA GCGGGCCCTG 
GACCACATCA CCACGCTGCT GAGGCCTGGG GGGCACCTCC TCCTCATCGG GGCCCTGGAG 
GAGTCGTGGT ACCTGGCTGG GGAGGCCAGG CTGACGGTGG TGCCAGTGTC TGAGGAGGAG 
GTGAGGGAGG CCCTGGTGCG TAGTGGCTAC AAGGTCCGGG ACCTCCGCAC CTATATCATG 
CCTGCCCACC TTCAGACAGG CGTAGATGAT GTCAAGGGCG TCTTCTTCGC CTGGGCTCAG 
AAGGTTGGGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523211.1
CDS	426..980
Translation	MTDFLEVNRQELGRWLQEEPGAFNWSMYSQHACLIEGKGECWQDKERQLRARVKRVLPIDVHQPQPLGAGSPAPLPADALVSAFCLEAVSPDLASFQRALDHITTLLRPGGHLLLIGALEESWYLAGEARLTVVPVSEEEVREALVRSGYKVRDLRTYIMPAHLQTGVDDVKGVFFAWAQKVGL

Target ORF information:

RefSeq Version	XM_011524909.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phenylethanolamine N-methyltransferase (PNMT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011524909.2

ORF Insert Sequence:

ATGACAGATT TCCTGGAGGT CAACCGCCAG GAGCTGGGGC GCTGGCTGCA GGAGGAGCCG 
GGGGCCTTCA ACTGGAGCAT GTACAGCCAA CATGCCTGCC TCATTGAGGG CAAGGGGGAA 
TGCTGGCAGG ATAAGGAGCG CCAGCTGCGA GCCAGGGTGA AACGGGTCCT GCCCATCGAC 
GTGCACCAGC CCCAGCCCCT GGGTGCTGGG AGCCCAGCTC CCCTGCCTGC TGACGCCCTG 
GTCTCTGCCT TCTGCTTGGA GGCTGTGAGC CCAGATCTTG CCAGCTTTCA GCGGGCCCTG 
GACCACATCA CCACGCTGCT GAGGCCTGGG GGGCACCTCC TCCTCATCGG GGCCCTGGAG 
GAGTCGTGGT ACCTGGCTGG GGAGGCCAGG CTGACGGTGG TGCCAGTGTC TGAGGAGGAG 
GTGAGGGAGG CCCTGGTGCG TAGTGGCTAC AAGGTCCGGG ACCTCCGCAC CTATATCATG 
CCTGCCCACC TTCAGACAGG CGTAGATGAT GTCAAGGGCG TCTTCTTCGC CTGGGCTCAG 
AAGGTTGGGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phenylethanolamine N-methyltransferase downregulation is associated with malignant pheochromocytoma/paraganglioma.
Oncotarget7(17)24141-53(2016 Apr)
Lee SE,Oh E,Lee B,Kim YJ,Oh DY,Jung K,Choi JS,Kim J,Kim SJ,Yang JW,An J,Oh YL,Choi YL

Kinetic and pH studies on human phenylethanolamine N-methyltransferase.
Archives of biochemistry and biophysics539(1)1-8(2013 Nov)
Wu Q,McLeish MJ

Phenylethanolamine N-methyltransferase gene promoter haplotypes and risk of essential hypertension.
American journal of hypertension24(11)1222-6(2011 Nov)
Huang C,Zhang S,Hu K,Ma Q,Yang T

A single human cell expresses all messenger ribonucleic acids: the arrow of time in a cell.
Molecular & general genetics : MGG258(3)233-9(1998 May)
Kimoto Y

Structure of human phenylethanolamine n-methyltransferase gene: existence of two types of mRNA with different transcription initiation sites.
Neurochemistry international15(4)555-65(1989)
Sasaoka T,Kaneda N,Kurosawa Y,Fujita K,Nagatsu T

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PNMT cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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