TSPEAR cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TSPEAR
Entrez Gene ID	54084
Full Name	thrombospondin type laminin G domain and EAR repeats
Synonyms	C21orf29,DFNB98,TSP-EAR
General protein information	Preferred Names thrombospondin type laminin G domain and EAR repeats Names thrombospondin-type laminin G domain and EAR repeat-containing protein thrombospondin-type laminin G domain and EAR repeats-containing protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 21 Map Location: 21q22.3
Summary	This gene encodes a protein that contains a N-terminal thrombospondin-type laminin G domain and several tandem arranged epilepsy-associated repeats (EARs). A mutation in this gene is the cause of autosomal recessive deafness-98. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012].
Disorder MIM:	612920
Disorder Html:	Deafness, autosomal recessive 98, 614861 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_144991.2	NP_659428.2	thrombospondin-type laminin G domain and EAR repeat-containing protein isoform 1 precursor
NM_144991.3	NP_659428.2	thrombospondin-type laminin G domain and EAR repeat-containing protein isoform 1 precursor
NM_001272037.2	NP_001258966.1	thrombospondin-type laminin G domain and EAR repeat-containing protein isoform 2
NM_001272037.1	NP_001258966.1	thrombospondin-type laminin G domain and EAR repeat-containing protein isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TSPEAR	XP_531582.2
Danio rerio (zebrafish)	tspearb	XP_682770.2
Danio rerio (zebrafish)	tspeara	XP_698991.2
Homo sapiens (human)	TSPEAR	NP_659428.2
Canis lupus familiaris (dog)	LOC611461	XP_005639060.1
Mus musculus (house mouse)	Tspear	NP_001274003.1
Gallus gallus (chicken)	TSPEAR	XP_422647.3
Bos taurus (cattle)	TSPEAR	NP_001193739.1
Xenopus tropicalis (tropical clawed frog)	tspear	XP_002936569.2

Gene Ontology
Bibliography

Related articles in PubMed

Human gingiva transcriptome during wound healing.
Wang Y, Tatakis DN
Journal of clinical periodontology44(4)394-402(2017 Apr)

Mutations in TSPEAR, Encoding a Regulator of Notch Signaling, Affect Tooth and Hair Follicle Morphogenesis.
Peled A, Sarig O, Samuelov L, Bertolini M, Ziv L, Weissglas-Volkov D, Eskin-Schwartz M, Adase CA, Malchin N, Bochner R, Fainberg G, Goldberg I, Sugawara K, Baniel A, Tsuruta D, Luxenburg C, Adir N, Duverger O, Morasso M, Shalev S, Gallo RL, Shomron N, Paus R, Sprecher E
PLoS genetics12(10)e1006369(2016 Oct)

Defect in the gene encoding the EAR/EPTP domain-containing protein TSPEAR causes DFNB98 profound deafness.
Delmaghani S, Aghaie A, Michalski N, Bonnet C, Weil D, Petit C
Human molecular genetics21(17)3835-44(2012 Sep)

A common protein interaction domain links two recently identified epilepsy genes.
Scheel H, Tomiuk S, Hofmann K
Human molecular genetics11(15)1757-62(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TSPEAR expression is significantly downregulated in human masticatory mucosa during wound healing
Title: Human gingiva transcriptome during wound healing.

using a luciferase-based reporter assay, we showed that TSPEAR knock-down is associated with decreased Notch signaling. In addition, NOTCH1 protein expression was reduced in patient scalp skin. Moreover, TSPEAR silencing in mouse hair follicle organ cultures was found to induce apoptosis in follicular epithelial cells, resulting in decreased hair bulb diameter
Title: Mutations in TSPEAR, Encoding a Regulator of Notch Signaling, Affect Tooth and Hair Follicle Morphogenesis.

TSPEAR, cause disorders with auditory features: epilepsy, which can include auditory features in humans; audiogenic seizures in animals; and/or hearing impairments in humans and mice.
Title: Defect in the gene encoding the EAR/EPTP domain-containing protein TSPEAR causes DFNB98 profound deafness.

The TSPEAR/C21orf29 promoter is activated by Trichostatin A (TSA) treatment according to promoter reporter assays in HEK 293 cells.
Title: Functional analysis and identification of cis-regulatory elements of human chromosome 21 gene promoters.

The following TSPEAR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSPEAR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu08639	NM_001272037.2 Latest version!	Homo sapiens thrombospondin type laminin G domain and EAR repeats (TSPEAR), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu18118	NM_144991.3 Latest version!	Homo sapiens thrombospondin type laminin G domain and EAR repeats (TSPEAR), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
View Old Accession Versions >>
OHu18118	NM_144991.2	Homo sapiens thrombospondin type laminin G domain and EAR repeats (TSPEAR), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu08639	NM_001272037.1	Homo sapiens thrombospondin type laminin G domain and EAR repeats (TSPEAR), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TSPEAR ( NM_001272037.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258966.1 Homo sapiens thrombospondin type laminin G domain and EAR repeats (TSPEAR), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu08639
Clone ID Related Accession (Same CDS sequence)	NM_001272037.2 , NM_001272037.1
Accession Version	NM_001272037.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	thrombospondin-type laminin G domain and EAR repeat-containing protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL773604.1, BC115414.1 and AJ487962.1. On May 31, 2019 this sequence version replaced NM_001272037.1. Transcript Variant: This variant (2) contains an additional exon in the 5' region that causes translation initiation from a downstream AUG compared to variant 1. The resulting isoform (2) has a shorter N-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC115414.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCTTCC CAGCATCCAG GATTTTCTCC CAGTGTGACC TCTTCCCTGA AGAATTTTCC 
ATCGTCGTAA CTTTGAGAGT TCCCAATCTT CCACCCAAGA GGAACGAGTA CCTGCTGACG 
GTGGTGGCAG AGGAGAGCGA CCTGCTGCTG CTCGGCCTGC GGTTGTCACC TGCCCAGCTG 
CACTTCCTGT TCCTTCGCGA GGACACGGCC GGCGCCTGGC AGACCCGAGT GTCCTTCCGC 
AGCCCGGCCC TGGTGGATGG CCGCTGGCAC ACACTGGTCC TGGCTGTGTC CGCAGGCGTC 
TTCTCCCTCA CCACGGACTG CGGCCTCCCG GTGGACATAA TGGCCGATGT GCCCTTCCCA 
GCCACCCTGT CAGTGAAAGG AGCTCGATTC TTCGTCGGCA GCCGGAGGAG AGCCAAAGGC 
CTGTTCATGG GACTGGTGAG GCAACTGGTC CTGCTGCCGG GCTCAGACGC CACCCCAAGG 
CTGTGTCCCA GCAGGAACGC CCCGCTGGCG GTGCTGTCCA TCCCACGGGT CCTGCAGGCT 
CTCACGGGGA AGCCAGAAGA TAACGAGGTG CTAAAATATC CCTATGAAAC CAACATTCGA 
GTGACGCTGG GACCCCAGCC ACCGTGTACC GAGGTGGAAG ACGCCCAGTT CTGGTTTGAT 
GCCAGCCGGA AGGGCCTGTA TCTGTGTGTT GGCAACGAGT GGGTCTCCGT GTTAGCAGCC 
AAAGAAAGAC TGGACTACGT GGAGGAGCAT CAGAACTTGT CCACCAACTC AGAGACCCTG 
GGCATTGAGG TGTTCCGCAT CCCTCAGGTG GGGCTCTTTG TGGCCACAGC CAATCGCAAA 
GCCACATCCG CCGTCTACAA GTGGACCGAA GAGAAGTTCG TCTCATATCA GAACATCCCC 
ACGCACCAAG CACAGGCCTG GAGGCATTTC ACCATCGGGA AAAAGATCTT CCTGGCAGTG 
GCTAATTTTG AACCAGATGA GAAGGGTCAG GAGTTCTCTG TCATTTACAA ATGGAGCCAC 
AGAAAGCTGA AGTTTACCCC ATATCAGAGC ATTGCCACAC ACAGCGCCCG AGACTGGGAG 
GCCTTCGAGG TGGATGGGGA GCACTTCCTG GCGGTGGCCA ACCACCGGGA AGGCGACAAC 
CACAACATCG ACAGTGTCAT CTACAAGTGG AACCCGGCAA CCCGGCTCTT CGAGGCCAAC 
CAGACCATCG CCACCTCCGG CGCCTACGAC TGGGAGTTCT TCAGTGTGGG GCCCTACTCG 
TTCCTGGTGG TGGCCAACAC CTTCAACGGC ACCTCCACCA AGGTGCACTC GCACCTCTAC 
ATCCGACTCC TGGGCTCCTT CCAGCTCTTC CAGTCCTTCC CGACGTTCGG TGCTGCAGAC 
TGGGAGGTCT TCCAGATCGG GGAGAGGATC TTCCTCGCTG TGGCAAACAG TCACAGCTAC 
GATGTGGAGA TGCAAGTCCA GAATGATTCC TATGTCATCA ACTCCGTCAT CTACGAGCTG 
AACGTGACCG CGCAGGCCTT TGTCAAGTTC CAGGACATTC TCACCTGCAG TGCTCTGGAC 
TGGGAGTTTT TCTCGGTGGG AGAAGATTAT TTCCTGGTGG TGGCCAACTC CTTCGATGGG 
CGTACCTTCT CGGTGAACAG TATTATTTAC AGGTGGCAGG GCTACGAGGG CTTCGTGGCG 
GTGCACAGCC TCCCCACCGT CGGCTGCAGG GACTGGGAGG CCTTCAGCAC CACGGCTGGT 
GCCTACCTCA TCTACTCCAG CGCCAAGGAG CCCCTCTCCA GGGTCCTGCG GCTGAGGACA 
CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258966.1
CDS	325..2130
Translation	MSFPASRIFSQCDLFPEEFSIVVTLRVPNLPPKRNEYLLTVVAEESDLLLLGLRLSPAQLHFLFLREDTAGAWQTRVSFRSPALVDGRWHTLVLAVSAGVFSLTTDCGLPVDIMADVPFPATLSVKGARFFVGSRRRAKGLFMGLVRQLVLLPGSDATPRLCPSRNAPLAVLSIPRVLQALTGKPEDNEVLKYPYETNIRVTLGPQPPCTEVEDAQFWFDASRKGLYLCVGNEWVSVLAAKERLDYVEEHQNLSTNSETLGIEVFRIPQVGLFVATANRKATSAVYKWTEEKFVSYQNIPTHQAQAWRHFTIGKKIFLAVANFEPDEKGQEFSVIYKWSHRKLKFTPYQSIATHSARDWEAFEVDGEHFLAVANHREGDNHNIDSVIYKWNPATRLFEANQTIATSGAYDWEFFSVGPYSFLVVANTFNGTSTKVHSHLYIRLLGSFQLFQSFPTFGAADWEVFQIGERIFLAVANSHSYDVEMQVQNDSYVINSVIYELNVTAQAFVKFQDILTCSALDWEFFSVGEDYFLVVANSFDGRTFSVNSIIYRWQGYEGFVAVHSLPTVGCRDWEAFSTTAGAYLIYSSAKEPLSRVLRLRTR

Target ORF information:

RefSeq Version	NM_001272037.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thrombospondin type laminin G domain and EAR repeats (TSPEAR), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272037.2

ORF Insert Sequence:

ATGAGCTTCC CAGCATCCAG GATTTTCTCC CAGTGTGACC TCTTCCCTGA AGAATTTTCC 
ATCGTCGTAA CTTTGAGAGT TCCCAATCTT CCACCCAAGA GGAACGAGTA CCTGCTGACG 
GTGGTGGCAG AGGAGAGCGA CCTGCTGCTG CTCGGCCTGC GGTTGTCACC TGCCCAGCTG 
CACTTCCTGT TCCTTCGCGA GGACACGGCC GGCGCCTGGC AGACCCGAGT GTCCTTCCGC 
AGCCCGGCCC TGGTGGATGG CCGCTGGCAC ACACTGGTCC TGGCTGTGTC CGCAGGCGTC 
TTCTCCCTCA CCACGGACTG CGGCCTCCCG GTGGACATAA TGGCCGATGT GCCCTTCCCA 
GCCACCCTGT CAGTGAAAGG AGCTCGATTC TTCGTCGGCA GCCGGAGGAG AGCCAAAGGC 
CTGTTCATGG GACTGGTGAG GCAACTGGTC CTGCTGCCGG GCTCAGACGC CACCCCAAGG 
CTGTGTCCCA GCAGGAACGC CCCGCTGGCG GTGCTGTCCA TCCCACGGGT CCTGCAGGCT 
CTCACGGGGA AGCCAGAAGA TAACGAGGTG CTAAAATATC CCTATGAAAC CAACATTCGA 
GTGACGCTGG GACCCCAGCC ACCGTGTACC GAGGTGGAAG ACGCCCAGTT CTGGTTTGAT 
GCCAGCCGGA AGGGCCTGTA TCTGTGTGTT GGCAACGAGT GGGTCTCCGT GTTAGCAGCC 
AAAGAAAGAC TGGACTACGT GGAGGAGCAT CAGAACTTGT CCACCAACTC AGAGACCCTG 
GGCATTGAGG TGTTCCGCAT CCCTCAGGTG GGGCTCTTTG TGGCCACAGC CAATCGCAAA 
GCCACATCCG CCGTCTACAA GTGGACCGAA GAGAAGTTCG TCTCATATCA GAACATCCCC 
ACGCACCAAG CACAGGCCTG GAGGCATTTC ACCATCGGGA AAAAGATCTT CCTGGCAGTG 
GCTAATTTTG AACCAGATGA GAAGGGTCAG GAGTTCTCTG TCATTTACAA ATGGAGCCAC 
AGAAAGCTGA AGTTTACCCC ATATCAGAGC ATTGCCACAC ACAGCGCCCG AGACTGGGAG 
GCCTTCGAGG TGGATGGGGA GCACTTCCTG GCGGTGGCCA ACCACCGGGA AGGCGACAAC 
CACAACATCG ACAGTGTCAT CTACAAGTGG AACCCGGCAA CCCGGCTCTT CGAGGCCAAC 
CAGACCATCG CCACCTCCGG CGCCTACGAC TGGGAGTTCT TCAGTGTGGG GCCCTACTCG 
TTCCTGGTGG TGGCCAACAC CTTCAACGGC ACCTCCACCA AGGTGCACTC GCACCTCTAC 
ATCCGACTCC TGGGCTCCTT CCAGCTCTTC CAGTCCTTCC CGACGTTCGG TGCTGCAGAC 
TGGGAGGTCT TCCAGATCGG GGAGAGGATC TTCCTCGCTG TGGCAAACAG TCACAGCTAC 
GATGTGGAGA TGCAAGTCCA GAATGATTCC TATGTCATCA ACTCCGTCAT CTACGAGCTG 
AACGTGACCG CGCAGGCCTT TGTCAAGTTC CAGGACATTC TCACCTGCAG TGCTCTGGAC 
TGGGAGTTTT TCTCGGTGGG AGAAGATTAT TTCCTGGTGG TGGCCAACTC CTTCGATGGG 
CGTACCTTCT CGGTGAACAG TATTATTTAC AGGTGGCAGG GCTACGAGGG CTTCGTGGCG 
GTGCACAGCC TCCCCACCGT CGGCTGCAGG GACTGGGAGG CCTTCAGCAC CACGGCTGGT 
GCCTACCTCA TCTACTCCAG CGCCAAGGAG CCCCTCTCCA GGGTCCTGCG GCTGAGGACA 
CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSPEAR ( NM_001272037.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258966.1 Homo sapiens thrombospondin type laminin G domain and EAR repeats (TSPEAR), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu08639
Clone ID Related Accession (Same CDS sequence)	NM_001272037.2 , NM_001272037.1
Accession Version	NM_001272037.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-11
Organism	Homo sapiens(human)
Product	thrombospondin-type laminin G domain and EAR repeat-containing protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL773604.1, BC115414.1 and AJ487962.1. Transcript Variant: This variant (2) contains an additional exon in the 5' region that causes translation initiation from a downstream AUG compared to variant 1. The resulting isoform (2) has a shorter N-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC115414.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCTTCC CAGCATCCAG GATTTTCTCC CAGTGTGACC TCTTCCCTGA AGAATTTTCC 
ATCGTCGTAA CTTTGAGAGT TCCCAATCTT CCACCCAAGA GGAACGAGTA CCTGCTGACG 
GTGGTGGCAG AGGAGAGCGA CCTGCTGCTG CTCGGCCTGC GGTTGTCACC TGCCCAGCTG 
CACTTCCTGT TCCTTCGCGA GGACACGGCC GGCGCCTGGC AGACCCGAGT GTCCTTCCGC 
AGCCCGGCCC TGGTGGATGG CCGCTGGCAC ACACTGGTCC TGGCTGTGTC CGCAGGCGTC 
TTCTCCCTCA CCACGGACTG CGGCCTCCCG GTGGACATAA TGGCCGATGT GCCCTTCCCA 
GCCACCCTGT CAGTGAAAGG AGCTCGATTC TTCGTCGGCA GCCGGAGGAG AGCCAAAGGC 
CTGTTCATGG GACTGGTGAG GCAACTGGTC CTGCTGCCGG GCTCAGACGC CACCCCAAGG 
CTGTGTCCCA GCAGGAACGC CCCGCTGGCG GTGCTGTCCA TCCCACGGGT CCTGCAGGCT 
CTCACGGGGA AGCCAGAAGA TAACGAGGTG CTAAAATATC CCTATGAAAC CAACATTCGA 
GTGACGCTGG GACCCCAGCC ACCGTGTACC GAGGTGGAAG ACGCCCAGTT CTGGTTTGAT 
GCCAGCCGGA AGGGCCTGTA TCTGTGTGTT GGCAACGAGT GGGTCTCCGT GTTAGCAGCC 
AAAGAAAGAC TGGACTACGT GGAGGAGCAT CAGAACTTGT CCACCAACTC AGAGACCCTG 
GGCATTGAGG TGTTCCGCAT CCCTCAGGTG GGGCTCTTTG TGGCCACAGC CAATCGCAAA 
GCCACATCCG CCGTCTACAA GTGGACCGAA GAGAAGTTCG TCTCATATCA GAACATCCCC 
ACGCACCAAG CACAGGCCTG GAGGCATTTC ACCATCGGGA AAAAGATCTT CCTGGCAGTG 
GCTAATTTTG AACCAGATGA GAAGGGTCAG GAGTTCTCTG TCATTTACAA ATGGAGCCAC 
AGAAAGCTGA AGTTTACCCC ATATCAGAGC ATTGCCACAC ACAGCGCCCG AGACTGGGAG 
GCCTTCGAGG TGGATGGGGA GCACTTCCTG GCGGTGGCCA ACCACCGGGA AGGCGACAAC 
CACAACATCG ACAGTGTCAT CTACAAGTGG AACCCGGCAA CCCGGCTCTT CGAGGCCAAC 
CAGACCATCG CCACCTCCGG CGCCTACGAC TGGGAGTTCT TCAGTGTGGG GCCCTACTCG 
TTCCTGGTGG TGGCCAACAC CTTCAACGGC ACCTCCACCA AGGTGCACTC GCACCTCTAC 
ATCCGACTCC TGGGCTCCTT CCAGCTCTTC CAGTCCTTCC CGACGTTCGG TGCTGCAGAC 
TGGGAGGTCT TCCAGATCGG GGAGAGGATC TTCCTCGCTG TGGCAAACAG TCACAGCTAC 
GATGTGGAGA TGCAAGTCCA GAATGATTCC TATGTCATCA ACTCCGTCAT CTACGAGCTG 
AACGTGACCG CGCAGGCCTT TGTCAAGTTC CAGGACATTC TCACCTGCAG TGCTCTGGAC 
TGGGAGTTTT TCTCGGTGGG AGAAGATTAT TTCCTGGTGG TGGCCAACTC CTTCGATGGG 
CGTACCTTCT CGGTGAACAG TATTATTTAC AGGTGGCAGG GCTACGAGGG CTTCGTGGCG 
GTGCACAGCC TCCCCACCGT CGGCTGCAGG GACTGGGAGG CCTTCAGCAC CACGGCTGGT 
GCCTACCTCA TCTACTCCAG CGCCAAGGAG CCCCTCTCCA GGGTCCTGCG GCTGAGGACA 
CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258966.1
CDS	333..2138
Translation	MSFPASRIFSQCDLFPEEFSIVVTLRVPNLPPKRNEYLLTVVAEESDLLLLGLRLSPAQLHFLFLREDTAGAWQTRVSFRSPALVDGRWHTLVLAVSAGVFSLTTDCGLPVDIMADVPFPATLSVKGARFFVGSRRRAKGLFMGLVRQLVLLPGSDATPRLCPSRNAPLAVLSIPRVLQALTGKPEDNEVLKYPYETNIRVTLGPQPPCTEVEDAQFWFDASRKGLYLCVGNEWVSVLAAKERLDYVEEHQNLSTNSETLGIEVFRIPQVGLFVATANRKATSAVYKWTEEKFVSYQNIPTHQAQAWRHFTIGKKIFLAVANFEPDEKGQEFSVIYKWSHRKLKFTPYQSIATHSARDWEAFEVDGEHFLAVANHREGDNHNIDSVIYKWNPATRLFEANQTIATSGAYDWEFFSVGPYSFLVVANTFNGTSTKVHSHLYIRLLGSFQLFQSFPTFGAADWEVFQIGERIFLAVANSHSYDVEMQVQNDSYVINSVIYELNVTAQAFVKFQDILTCSALDWEFFSVGEDYFLVVANSFDGRTFSVNSIIYRWQGYEGFVAVHSLPTVGCRDWEAFSTTAGAYLIYSSAKEPLSRVLRLRTR

Target ORF information:

RefSeq Version	NM_001272037.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thrombospondin type laminin G domain and EAR repeats (TSPEAR), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272037.1

ORF Insert Sequence:

ATGAGCTTCC CAGCATCCAG GATTTTCTCC CAGTGTGACC TCTTCCCTGA AGAATTTTCC 
ATCGTCGTAA CTTTGAGAGT TCCCAATCTT CCACCCAAGA GGAACGAGTA CCTGCTGACG 
GTGGTGGCAG AGGAGAGCGA CCTGCTGCTG CTCGGCCTGC GGTTGTCACC TGCCCAGCTG 
CACTTCCTGT TCCTTCGCGA GGACACGGCC GGCGCCTGGC AGACCCGAGT GTCCTTCCGC 
AGCCCGGCCC TGGTGGATGG CCGCTGGCAC ACACTGGTCC TGGCTGTGTC CGCAGGCGTC 
TTCTCCCTCA CCACGGACTG CGGCCTCCCG GTGGACATAA TGGCCGATGT GCCCTTCCCA 
GCCACCCTGT CAGTGAAAGG AGCTCGATTC TTCGTCGGCA GCCGGAGGAG AGCCAAAGGC 
CTGTTCATGG GACTGGTGAG GCAACTGGTC CTGCTGCCGG GCTCAGACGC CACCCCAAGG 
CTGTGTCCCA GCAGGAACGC CCCGCTGGCG GTGCTGTCCA TCCCACGGGT CCTGCAGGCT 
CTCACGGGGA AGCCAGAAGA TAACGAGGTG CTAAAATATC CCTATGAAAC CAACATTCGA 
GTGACGCTGG GACCCCAGCC ACCGTGTACC GAGGTGGAAG ACGCCCAGTT CTGGTTTGAT 
GCCAGCCGGA AGGGCCTGTA TCTGTGTGTT GGCAACGAGT GGGTCTCCGT GTTAGCAGCC 
AAAGAAAGAC TGGACTACGT GGAGGAGCAT CAGAACTTGT CCACCAACTC AGAGACCCTG 
GGCATTGAGG TGTTCCGCAT CCCTCAGGTG GGGCTCTTTG TGGCCACAGC CAATCGCAAA 
GCCACATCCG CCGTCTACAA GTGGACCGAA GAGAAGTTCG TCTCATATCA GAACATCCCC 
ACGCACCAAG CACAGGCCTG GAGGCATTTC ACCATCGGGA AAAAGATCTT CCTGGCAGTG 
GCTAATTTTG AACCAGATGA GAAGGGTCAG GAGTTCTCTG TCATTTACAA ATGGAGCCAC 
AGAAAGCTGA AGTTTACCCC ATATCAGAGC ATTGCCACAC ACAGCGCCCG AGACTGGGAG 
GCCTTCGAGG TGGATGGGGA GCACTTCCTG GCGGTGGCCA ACCACCGGGA AGGCGACAAC 
CACAACATCG ACAGTGTCAT CTACAAGTGG AACCCGGCAA CCCGGCTCTT CGAGGCCAAC 
CAGACCATCG CCACCTCCGG CGCCTACGAC TGGGAGTTCT TCAGTGTGGG GCCCTACTCG 
TTCCTGGTGG TGGCCAACAC CTTCAACGGC ACCTCCACCA AGGTGCACTC GCACCTCTAC 
ATCCGACTCC TGGGCTCCTT CCAGCTCTTC CAGTCCTTCC CGACGTTCGG TGCTGCAGAC 
TGGGAGGTCT TCCAGATCGG GGAGAGGATC TTCCTCGCTG TGGCAAACAG TCACAGCTAC 
GATGTGGAGA TGCAAGTCCA GAATGATTCC TATGTCATCA ACTCCGTCAT CTACGAGCTG 
AACGTGACCG CGCAGGCCTT TGTCAAGTTC CAGGACATTC TCACCTGCAG TGCTCTGGAC 
TGGGAGTTTT TCTCGGTGGG AGAAGATTAT TTCCTGGTGG TGGCCAACTC CTTCGATGGG 
CGTACCTTCT CGGTGAACAG TATTATTTAC AGGTGGCAGG GCTACGAGGG CTTCGTGGCG 
GTGCACAGCC TCCCCACCGT CGGCTGCAGG GACTGGGAGG CCTTCAGCAC CACGGCTGGT 
GCCTACCTCA TCTACTCCAG CGCCAAGGAG CCCCTCTCCA GGGTCCTGCG GCTGAGGACA 
CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSPEAR ( NM_144991.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_659428.2 Homo sapiens thrombospondin type laminin G domain and EAR repeats (TSPEAR), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18118
Clone ID Related Accession (Same CDS sequence)	NM_144991.2 , NM_144991.3
Accession Version	NM_144991.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	thrombospondin-type laminin G domain and EAR repeat-containing protein isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ487962.1. This sequence is a reference standard in the RefSeqGene project. On Jul 29, 2002 this sequence version replaced NM_144991.1. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AJ487962.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCCC TGCTGAGTCT GTGTTTTGTG CTGCCCCTGG CGGCCCCCGG CCACGGCACG 
CAGGGTTGGG AGCCCTGCAC AGACCTGCGC CCCCTGGACA TCCTGGCGGA AGTGGTCCCT 
TCTGATGGCG CCACAAGCGG GATCAGGATA GTTCAGGTTC ACGGTGCACG GGGACTCCAG 
CTCTCAGTAG CCGCCCCCCG CACCATGAGC TTCCCAGCAT CCAGGATTTT CTCCCAGTGT 
GACCTCTTCC CTGAAGAATT TTCCATCGTC GTAACTTTGA GAGTTCCCAA TCTTCCACCC 
AAGAGGAACG AGTACCTGCT GACGGTGGTG GCAGAGGAGA GCGACCTGCT GCTGCTCGGC 
CTGCGGTTGT CACCTGCCCA GCTGCACTTC CTGTTCCTTC GCGAGGACAC GGCCGGCGCC 
TGGCAGACCC GAGTGTCCTT CCGCAGCCCG GCCCTGGTGG ATGGCCGCTG GCACACACTG 
GTCCTGGCTG TGTCCGCAGG CGTCTTCTCC CTCACCACGG ACTGCGGCCT CCCGGTGGAC 
ATAATGGCCG ATGTGCCCTT CCCAGCCACC CTGTCAGTGA AAGGAGCTCG ATTCTTCGTC 
GGCAGCCGGA GGAGAGCCAA AGGCCTGTTC ATGGGACTGG TGAGGCAACT GGTCCTGCTG 
CCGGGCTCAG ACGCCACCCC AAGGCTGTGT CCCAGCAGGA ACGCCCCGCT GGCGGTGCTG 
TCCATCCCAC GGGTCCTGCA GGCTCTCACG GGGAAGCCAG AAGATAACGA GGTGCTAAAA 
TATCCCTATG AAACCAACAT TCGAGTGACG CTGGGACCCC AGCCACCGTG TACCGAGGTG 
GAAGACGCCC AGTTCTGGTT TGATGCCAGC CGGAAGGGCC TGTATCTGTG TGTTGGCAAC 
GAGTGGGTCT CCGTGTTAGC AGCCAAAGAA AGACTGGACT ACGTGGAGGA GCATCAGAAC 
TTGTCCACCA ACTCAGAGAC CCTGGGCATT GAGGTGTTCC GCATCCCTCA GGTGGGGCTC 
TTTGTGGCCA CAGCCAATCG CAAAGCCACA TCCGCCGTCT ACAAGTGGAC CGAAGAGAAG 
TTCGTCTCAT ATCAGAACAT CCCCACGCAC CAAGCACAGG CCTGGAGGCA TTTCACCATC 
GGGAAAAAGA TCTTCCTGGC AGTGGCTAAT TTTGAACCAG ATGAGAAGGG TCAGGAGTTC 
TCTGTCATTT ACAAATGGAG CCACAGAAAG CTGAAGTTTA CCCCATATCA GAGCATTGCC 
ACACACAGCG CCCGAGACTG GGAGGCCTTC GAGGTGGATG GGGAGCACTT CCTGGCGGTG 
GCCAACCACC GGGAAGGCGA CAACCACAAC ATCGACAGTG TCATCTACAA GTGGAACCCG 
GCAACCCGGC TCTTCGAGGC CAACCAGACC ATCGCCACCT CCGGCGCCTA CGACTGGGAG 
TTCTTCAGTG TGGGGCCCTA CTCGTTCCTG GTGGTGGCCA ACACCTTCAA CGGCACCTCC 
ACCAAGGTGC ACTCGCACCT CTACATCCGA CTCCTGGGCT CCTTCCAGCT CTTCCAGTCC 
TTCCCGACGT TCGGTGCTGC AGACTGGGAG GTCTTCCAGA TCGGGGAGAG GATCTTCCTC 
GCTGTGGCAA ACAGTCACAG CTACGATGTG GAGATGCAAG TCCAGAATGA TTCCTATGTC 
ATCAACTCCG TCATCTACGA GCTGAACGTG ACCGCGCAGG CCTTTGTCAA GTTCCAGGAC 
ATTCTCACCT GCAGTGCTCT GGACTGGGAG TTTTTCTCGG TGGGAGAAGA TTATTTCCTG 
GTGGTGGCCA ACTCCTTCGA TGGGCGTACC TTCTCGGTGA ACAGTATTAT TTACAGGTGG 
CAGGGCTACG AGGGCTTCGT GGCGGTGCAC AGCCTCCCCA CCGTCGGCTG CAGGGACTGG 
GAGGCCTTCA GCACCACGGC TGGTGCCTAC CTCATCTACT CCAGCGCCAA GGAGCCCCTC 
TCCAGGGTCC TGCGGCTGAG GACACGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_659428.2
CDS	67..2076
Translation	MSALLSLCFVLPLAAPGHGTQGWEPCTDLRPLDILAEVVPSDGATSGIRIVQVHGARGLQLSVAAPRTMSFPASRIFSQCDLFPEEFSIVVTLRVPNLPPKRNEYLLTVVAEESDLLLLGLRLSPAQLHFLFLREDTAGAWQTRVSFRSPALVDGRWHTLVLAVSAGVFSLTTDCGLPVDIMADVPFPATLSVKGARFFVGSRRRAKGLFMGLVRQLVLLPGSDATPRLCPSRNAPLAVLSIPRVLQALTGKPEDNEVLKYPYETNIRVTLGPQPPCTEVEDAQFWFDASRKGLYLCVGNEWVSVLAAKERLDYVEEHQNLSTNSETLGIEVFRIPQVGLFVATANRKATSAVYKWTEEKFVSYQNIPTHQAQAWRHFTIGKKIFLAVANFEPDEKGQEFSVIYKWSHRKLKFTPYQSIATHSARDWEAFEVDGEHFLAVANHREGDNHNIDSVIYKWNPATRLFEANQTIATSGAYDWEFFSVGPYSFLVVANTFNGTSTKVHSHLYIRLLGSFQLFQSFPTFGAADWEVFQIGERIFLAVANSHSYDVEMQVQNDSYVINSVIYELNVTAQAFVKFQDILTCSALDWEFFSVGEDYFLVVANSFDGRTFSVNSIIYRWQGYEGFVAVHSLPTVGCRDWEAFSTTAGAYLIYSSAKEPLSRVLRLRTR

Target ORF information:

RefSeq Version	NM_144991.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thrombospondin type laminin G domain and EAR repeats (TSPEAR), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144991.2

ORF Insert Sequence:

ATGTCTGCCC TGCTGAGTCT GTGTTTTGTG CTGCCCCTGG CGGCCCCCGG CCACGGCACG 
CAGGGTTGGG AGCCCTGCAC AGACCTGCGC CCCCTGGACA TCCTGGCGGA AGTGGTCCCT 
TCTGATGGCG CCACAAGCGG GATCAGGATA GTTCAGGTTC ACGGTGCACG GGGACTCCAG 
CTCTCAGTAG CCGCCCCCCG CACCATGAGC TTCCCAGCAT CCAGGATTTT CTCCCAGTGT 
GACCTCTTCC CTGAAGAATT TTCCATCGTC GTAACTTTGA GAGTTCCCAA TCTTCCACCC 
AAGAGGAACG AGTACCTGCT GACGGTGGTG GCAGAGGAGA GCGACCTGCT GCTGCTCGGC 
CTGCGGTTGT CACCTGCCCA GCTGCACTTC CTGTTCCTTC GCGAGGACAC GGCCGGCGCC 
TGGCAGACCC GAGTGTCCTT CCGCAGCCCG GCCCTGGTGG ATGGCCGCTG GCACACACTG 
GTCCTGGCTG TGTCCGCAGG CGTCTTCTCC CTCACCACGG ACTGCGGCCT CCCGGTGGAC 
ATAATGGCCG ATGTGCCCTT CCCAGCCACC CTGTCAGTGA AAGGAGCTCG ATTCTTCGTC 
GGCAGCCGGA GGAGAGCCAA AGGCCTGTTC ATGGGACTGG TGAGGCAACT GGTCCTGCTG 
CCGGGCTCAG ACGCCACCCC AAGGCTGTGT CCCAGCAGGA ACGCCCCGCT GGCGGTGCTG 
TCCATCCCAC GGGTCCTGCA GGCTCTCACG GGGAAGCCAG AAGATAACGA GGTGCTAAAA 
TATCCCTATG AAACCAACAT TCGAGTGACG CTGGGACCCC AGCCACCGTG TACCGAGGTG 
GAAGACGCCC AGTTCTGGTT TGATGCCAGC CGGAAGGGCC TGTATCTGTG TGTTGGCAAC 
GAGTGGGTCT CCGTGTTAGC AGCCAAAGAA AGACTGGACT ACGTGGAGGA GCATCAGAAC 
TTGTCCACCA ACTCAGAGAC CCTGGGCATT GAGGTGTTCC GCATCCCTCA GGTGGGGCTC 
TTTGTGGCCA CAGCCAATCG CAAAGCCACA TCCGCCGTCT ACAAGTGGAC CGAAGAGAAG 
TTCGTCTCAT ATCAGAACAT CCCCACGCAC CAAGCACAGG CCTGGAGGCA TTTCACCATC 
GGGAAAAAGA TCTTCCTGGC AGTGGCTAAT TTTGAACCAG ATGAGAAGGG TCAGGAGTTC 
TCTGTCATTT ACAAATGGAG CCACAGAAAG CTGAAGTTTA CCCCATATCA GAGCATTGCC 
ACACACAGCG CCCGAGACTG GGAGGCCTTC GAGGTGGATG GGGAGCACTT CCTGGCGGTG 
GCCAACCACC GGGAAGGCGA CAACCACAAC ATCGACAGTG TCATCTACAA GTGGAACCCG 
GCAACCCGGC TCTTCGAGGC CAACCAGACC ATCGCCACCT CCGGCGCCTA CGACTGGGAG 
TTCTTCAGTG TGGGGCCCTA CTCGTTCCTG GTGGTGGCCA ACACCTTCAA CGGCACCTCC 
ACCAAGGTGC ACTCGCACCT CTACATCCGA CTCCTGGGCT CCTTCCAGCT CTTCCAGTCC 
TTCCCGACGT TCGGTGCTGC AGACTGGGAG GTCTTCCAGA TCGGGGAGAG GATCTTCCTC 
GCTGTGGCAA ACAGTCACAG CTACGATGTG GAGATGCAAG TCCAGAATGA TTCCTATGTC 
ATCAACTCCG TCATCTACGA GCTGAACGTG ACCGCGCAGG CCTTTGTCAA GTTCCAGGAC 
ATTCTCACCT GCAGTGCTCT GGACTGGGAG TTTTTCTCGG TGGGAGAAGA TTATTTCCTG 
GTGGTGGCCA ACTCCTTCGA TGGGCGTACC TTCTCGGTGA ACAGTATTAT TTACAGGTGG 
CAGGGCTACG AGGGCTTCGT GGCGGTGCAC AGCCTCCCCA CCGTCGGCTG CAGGGACTGG 
GAGGCCTTCA GCACCACGGC TGGTGCCTAC CTCATCTACT CCAGCGCCAA GGAGCCCCTC 
TCCAGGGTCC TGCGGCTGAG GACACGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSPEAR ( NM_144991.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_659428.2 Homo sapiens thrombospondin type laminin G domain and EAR repeats (TSPEAR), transcript variant 1, mRNA.

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CloneID	OHu18118
Clone ID Related Accession (Same CDS sequence)	NM_144991.2 , NM_144991.3
Accession Version	NM_144991.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	thrombospondin-type laminin G domain and EAR repeat-containing protein isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ487962.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_144991.2. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AJ487962.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000323084.9/ ENSP00000321987.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTGCCC TGCTGAGTCT GTGTTTTGTG CTGCCCCTGG CGGCCCCCGG CCACGGCACG 
CAGGGTTGGG AGCCCTGCAC AGACCTGCGC CCCCTGGACA TCCTGGCGGA AGTGGTCCCT 
TCTGATGGCG CCACAAGCGG GATCAGGATA GTTCAGGTTC ACGGTGCACG GGGACTCCAG 
CTCTCAGTAG CCGCCCCCCG CACCATGAGC TTCCCAGCAT CCAGGATTTT CTCCCAGTGT 
GACCTCTTCC CTGAAGAATT TTCCATCGTC GTAACTTTGA GAGTTCCCAA TCTTCCACCC 
AAGAGGAACG AGTACCTGCT GACGGTGGTG GCAGAGGAGA GCGACCTGCT GCTGCTCGGC 
CTGCGGTTGT CACCTGCCCA GCTGCACTTC CTGTTCCTTC GCGAGGACAC GGCCGGCGCC 
TGGCAGACCC GAGTGTCCTT CCGCAGCCCG GCCCTGGTGG ATGGCCGCTG GCACACACTG 
GTCCTGGCTG TGTCCGCAGG CGTCTTCTCC CTCACCACGG ACTGCGGCCT CCCGGTGGAC 
ATAATGGCCG ATGTGCCCTT CCCAGCCACC CTGTCAGTGA AAGGAGCTCG ATTCTTCGTC 
GGCAGCCGGA GGAGAGCCAA AGGCCTGTTC ATGGGACTGG TGAGGCAACT GGTCCTGCTG 
CCGGGCTCAG ACGCCACCCC AAGGCTGTGT CCCAGCAGGA ACGCCCCGCT GGCGGTGCTG 
TCCATCCCAC GGGTCCTGCA GGCTCTCACG GGGAAGCCAG AAGATAACGA GGTGCTAAAA 
TATCCCTATG AAACCAACAT TCGAGTGACG CTGGGACCCC AGCCACCGTG TACCGAGGTG 
GAAGACGCCC AGTTCTGGTT TGATGCCAGC CGGAAGGGCC TGTATCTGTG TGTTGGCAAC 
GAGTGGGTCT CCGTGTTAGC AGCCAAAGAA AGACTGGACT ACGTGGAGGA GCATCAGAAC 
TTGTCCACCA ACTCAGAGAC CCTGGGCATT GAGGTGTTCC GCATCCCTCA GGTGGGGCTC 
TTTGTGGCCA CAGCCAATCG CAAAGCCACA TCCGCCGTCT ACAAGTGGAC CGAAGAGAAG 
TTCGTCTCAT ATCAGAACAT CCCCACGCAC CAAGCACAGG CCTGGAGGCA TTTCACCATC 
GGGAAAAAGA TCTTCCTGGC AGTGGCTAAT TTTGAACCAG ATGAGAAGGG TCAGGAGTTC 
TCTGTCATTT ACAAATGGAG CCACAGAAAG CTGAAGTTTA CCCCATATCA GAGCATTGCC 
ACACACAGCG CCCGAGACTG GGAGGCCTTC GAGGTGGATG GGGAGCACTT CCTGGCGGTG 
GCCAACCACC GGGAAGGCGA CAACCACAAC ATCGACAGTG TCATCTACAA GTGGAACCCG 
GCAACCCGGC TCTTCGAGGC CAACCAGACC ATCGCCACCT CCGGCGCCTA CGACTGGGAG 
TTCTTCAGTG TGGGGCCCTA CTCGTTCCTG GTGGTGGCCA ACACCTTCAA CGGCACCTCC 
ACCAAGGTGC ACTCGCACCT CTACATCCGA CTCCTGGGCT CCTTCCAGCT CTTCCAGTCC 
TTCCCGACGT TCGGTGCTGC AGACTGGGAG GTCTTCCAGA TCGGGGAGAG GATCTTCCTC 
GCTGTGGCAA ACAGTCACAG CTACGATGTG GAGATGCAAG TCCAGAATGA TTCCTATGTC 
ATCAACTCCG TCATCTACGA GCTGAACGTG ACCGCGCAGG CCTTTGTCAA GTTCCAGGAC 
ATTCTCACCT GCAGTGCTCT GGACTGGGAG TTTTTCTCGG TGGGAGAAGA TTATTTCCTG 
GTGGTGGCCA ACTCCTTCGA TGGGCGTACC TTCTCGGTGA ACAGTATTAT TTACAGGTGG 
CAGGGCTACG AGGGCTTCGT GGCGGTGCAC AGCCTCCCCA CCGTCGGCTG CAGGGACTGG 
GAGGCCTTCA GCACCACGGC TGGTGCCTAC CTCATCTACT CCAGCGCCAA GGAGCCCCTC 
TCCAGGGTCC TGCGGCTGAG GACACGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_659428.2
CDS	59..2068
Translation	MSALLSLCFVLPLAAPGHGTQGWEPCTDLRPLDILAEVVPSDGATSGIRIVQVHGARGLQLSVAAPRTMSFPASRIFSQCDLFPEEFSIVVTLRVPNLPPKRNEYLLTVVAEESDLLLLGLRLSPAQLHFLFLREDTAGAWQTRVSFRSPALVDGRWHTLVLAVSAGVFSLTTDCGLPVDIMADVPFPATLSVKGARFFVGSRRRAKGLFMGLVRQLVLLPGSDATPRLCPSRNAPLAVLSIPRVLQALTGKPEDNEVLKYPYETNIRVTLGPQPPCTEVEDAQFWFDASRKGLYLCVGNEWVSVLAAKERLDYVEEHQNLSTNSETLGIEVFRIPQVGLFVATANRKATSAVYKWTEEKFVSYQNIPTHQAQAWRHFTIGKKIFLAVANFEPDEKGQEFSVIYKWSHRKLKFTPYQSIATHSARDWEAFEVDGEHFLAVANHREGDNHNIDSVIYKWNPATRLFEANQTIATSGAYDWEFFSVGPYSFLVVANTFNGTSTKVHSHLYIRLLGSFQLFQSFPTFGAADWEVFQIGERIFLAVANSHSYDVEMQVQNDSYVINSVIYELNVTAQAFVKFQDILTCSALDWEFFSVGEDYFLVVANSFDGRTFSVNSIIYRWQGYEGFVAVHSLPTVGCRDWEAFSTTAGAYLIYSSAKEPLSRVLRLRTR

Target ORF information:

RefSeq Version	NM_144991.3
Organism	Homo sapiens(human)
Definition	Homo sapiens thrombospondin type laminin G domain and EAR repeats (TSPEAR), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144991.3

ORF Insert Sequence:

ATGTCTGCCC TGCTGAGTCT GTGTTTTGTG CTGCCCCTGG CGGCCCCCGG CCACGGCACG 
CAGGGTTGGG AGCCCTGCAC AGACCTGCGC CCCCTGGACA TCCTGGCGGA AGTGGTCCCT 
TCTGATGGCG CCACAAGCGG GATCAGGATA GTTCAGGTTC ACGGTGCACG GGGACTCCAG 
CTCTCAGTAG CCGCCCCCCG CACCATGAGC TTCCCAGCAT CCAGGATTTT CTCCCAGTGT 
GACCTCTTCC CTGAAGAATT TTCCATCGTC GTAACTTTGA GAGTTCCCAA TCTTCCACCC 
AAGAGGAACG AGTACCTGCT GACGGTGGTG GCAGAGGAGA GCGACCTGCT GCTGCTCGGC 
CTGCGGTTGT CACCTGCCCA GCTGCACTTC CTGTTCCTTC GCGAGGACAC GGCCGGCGCC 
TGGCAGACCC GAGTGTCCTT CCGCAGCCCG GCCCTGGTGG ATGGCCGCTG GCACACACTG 
GTCCTGGCTG TGTCCGCAGG CGTCTTCTCC CTCACCACGG ACTGCGGCCT CCCGGTGGAC 
ATAATGGCCG ATGTGCCCTT CCCAGCCACC CTGTCAGTGA AAGGAGCTCG ATTCTTCGTC 
GGCAGCCGGA GGAGAGCCAA AGGCCTGTTC ATGGGACTGG TGAGGCAACT GGTCCTGCTG 
CCGGGCTCAG ACGCCACCCC AAGGCTGTGT CCCAGCAGGA ACGCCCCGCT GGCGGTGCTG 
TCCATCCCAC GGGTCCTGCA GGCTCTCACG GGGAAGCCAG AAGATAACGA GGTGCTAAAA 
TATCCCTATG AAACCAACAT TCGAGTGACG CTGGGACCCC AGCCACCGTG TACCGAGGTG 
GAAGACGCCC AGTTCTGGTT TGATGCCAGC CGGAAGGGCC TGTATCTGTG TGTTGGCAAC 
GAGTGGGTCT CCGTGTTAGC AGCCAAAGAA AGACTGGACT ACGTGGAGGA GCATCAGAAC 
TTGTCCACCA ACTCAGAGAC CCTGGGCATT GAGGTGTTCC GCATCCCTCA GGTGGGGCTC 
TTTGTGGCCA CAGCCAATCG CAAAGCCACA TCCGCCGTCT ACAAGTGGAC CGAAGAGAAG 
TTCGTCTCAT ATCAGAACAT CCCCACGCAC CAAGCACAGG CCTGGAGGCA TTTCACCATC 
GGGAAAAAGA TCTTCCTGGC AGTGGCTAAT TTTGAACCAG ATGAGAAGGG TCAGGAGTTC 
TCTGTCATTT ACAAATGGAG CCACAGAAAG CTGAAGTTTA CCCCATATCA GAGCATTGCC 
ACACACAGCG CCCGAGACTG GGAGGCCTTC GAGGTGGATG GGGAGCACTT CCTGGCGGTG 
GCCAACCACC GGGAAGGCGA CAACCACAAC ATCGACAGTG TCATCTACAA GTGGAACCCG 
GCAACCCGGC TCTTCGAGGC CAACCAGACC ATCGCCACCT CCGGCGCCTA CGACTGGGAG 
TTCTTCAGTG TGGGGCCCTA CTCGTTCCTG GTGGTGGCCA ACACCTTCAA CGGCACCTCC 
ACCAAGGTGC ACTCGCACCT CTACATCCGA CTCCTGGGCT CCTTCCAGCT CTTCCAGTCC 
TTCCCGACGT TCGGTGCTGC AGACTGGGAG GTCTTCCAGA TCGGGGAGAG GATCTTCCTC 
GCTGTGGCAA ACAGTCACAG CTACGATGTG GAGATGCAAG TCCAGAATGA TTCCTATGTC 
ATCAACTCCG TCATCTACGA GCTGAACGTG ACCGCGCAGG CCTTTGTCAA GTTCCAGGAC 
ATTCTCACCT GCAGTGCTCT GGACTGGGAG TTTTTCTCGG TGGGAGAAGA TTATTTCCTG 
GTGGTGGCCA ACTCCTTCGA TGGGCGTACC TTCTCGGTGA ACAGTATTAT TTACAGGTGG 
CAGGGCTACG AGGGCTTCGT GGCGGTGCAC AGCCTCCCCA CCGTCGGCTG CAGGGACTGG 
GAGGCCTTCA GCACCACGGC TGGTGCCTAC CTCATCTACT CCAGCGCCAA GGAGCCCCTC 
TCCAGGGTCC TGCGGCTGAG GACACGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human gingiva transcriptome during wound healing.
Journal of clinical periodontology44(4)394-402(2017 Apr)
Wang Y,Tatakis DN

Mutations in TSPEAR, Encoding a Regulator of Notch Signaling, Affect Tooth and Hair Follicle Morphogenesis.
PLoS genetics12(10)e1006369(2016 Oct)
Peled A,Sarig O,Samuelov L,Bertolini M,Ziv L,Weissglas-Volkov D,Eskin-Schwartz M,Adase CA,Malchin N,Bochner R,Fainberg G,Goldberg I,Sugawara K,Baniel A,Tsuruta D,Luxenburg C,Adir N,Duverger O,Morasso M,Shalev S,Gallo RL,Shomron N,Paus R,Sprecher E

Defect in the gene encoding the EAR/EPTP domain-containing protein TSPEAR causes DFNB98 profound deafness.
Human molecular genetics21(17)3835-44(2012 Sep)
Delmaghani S,Aghaie A,Michalski N,Bonnet C,Weil D,Petit C

A common protein interaction domain links two recently identified epilepsy genes.
Human molecular genetics11(15)1757-62(2002 Jul)
Scheel H,Tomiuk S,Hofmann K

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TSPEAR cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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