BRWD1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRWD1
Entrez Gene ID	54014
Full Name	bromodomain and WD repeat domain containing 1
Synonyms	C21orf107,DCAF19,N143,WDR9,WRD9
General protein information	Preferred Names bromodomain and WD repeat domain containing 1 Names bromodomain and WD repeat-containing protein 1 WD repeat protein WDR9-form2 WD repeat-containing protein 9 transcriptional unit N143
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 21 Map Location: 21q22.2
Summary	This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD) residues which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 2 bromodomains and multiple WD repeats. This gene is located within the Down syndrome region-2 on chromosome 21. Alternative splicing of this gene generates multiple transcript variants encoding distinct isoforms. In mouse, this gene encodes a nuclear protein that has a polyglutamine-containing region that functions as a transcriptional activation domain which may regulate chromatin remodelling and associates with a component of the SWI/SNF chromatin remodelling complex.[provided by RefSeq, Jun 2011].
Disorder MIM:	617824

mRNA and Protein(s)

mRNA	Protein	Name
NM_018963.4	NP_061836.2	bromodomain and WD repeat-containing protein 1 isoform A
XM_017028375.1	XP_016883864.1	bromodomain and WD repeat-containing protein 1 isoform X5
NM_033656.4	NP_387505.1	bromodomain and WD repeat-containing protein 1 isoform B
XM_017028374.1	XP_016883863.1	bromodomain and WD repeat-containing protein 1 isoform X4
NM_001007246.2	NP_001007247.1	bromodomain and WD repeat-containing protein 1 isoform C
XM_011529612.2	XP_011527914.1	bromodomain and WD repeat-containing protein 1 isoform X3
XM_011529611.1	XP_011527913.1	bromodomain and WD repeat-containing protein 1 isoform X1
NM_033656.3	NP_387505.1	bromodomain and WD repeat-containing protein 1 isoform B
NM_018963.5	NP_061836.2	bromodomain and WD repeat-containing protein 1 isoform A
XM_011529613.1	XP_011527915.1	bromodomain and WD repeat-containing protein 1 isoform X4
XM_017028373.1	XP_016883862.1	bromodomain and WD repeat-containing protein 1 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	BRWD1	XP_001170924.1
Gallus gallus (chicken)	BRWD1	XP_416730.4
Canis lupus familiaris (dog)	BRWD1	XP_544889.4
Bos taurus (cattle)	BRWD1	NP_001096644.1
Homo sapiens (human)	BRWD1	NP_061836.2
Mus musculus (house mouse)	Brwd1	NP_660107.2
Rattus norvegicus (Norway rat)	Brwd1	XP_006248248.1
Danio rerio (zebrafish)	brwd1	XP_001920441.4
Xenopus tropicalis (tropical clawed frog)	brwd1	XP_002938777.2
	BRWD1	XP_001108760.2

Gene Ontology
Bibliography

Related articles in PubMed

Trisomy 21 Alters DNA Methylation in Parent-of-Origin-Dependent and -Independent Manners.
Alves da Silva AF, Machado FB, Pavarino ?C, Biselli-P?rico JM, Zampieri BL, da Silva Francisco Junior R, Mozer Rodrigues PT, Terra Machado D, Santos-Rebou?as CB, Gomes Fernandes M, Chuva de Sousa Lopes SM, Lopes Rios ?F, Medina-Acosta E
PloS one11(4)e0154108(2016)

Parent-of-origin-specific allelic associations among 106 genomic loci for age at menarche.
Perry JR, Day F, Elks CE, Sulem P, Thompson DJ, Ferreira T, He C, Chasman DI, Esko T, Thorleifsson G, Albrecht E, Ang WQ, Corre T, Cousminer DL, Feenstra B, Franceschini N, Ganna A, Johnson AD, Kjellqvist S, Lunetta KL, McMahon G, Nolte IM, Paternoster L, Porcu E, Smith AV, Stolk L, Teumer A, T?ernikova N, Tikkanen E, Ulivi S, Wagner EK, Amin N, Bierut LJ, Byrne EM, Hottenga JJ, Koller DL, Mangino M, Pers TH, Yerges-Armstrong LM, Zhao JH, Andrulis IL, Anton-Culver H, Atsma F, Bandinelli S, Beckmann MW, Benitez J, Blomqvist C, Bojesen SE, Bolla MK, Bonanni B, Brauch H, Brenner H, Buring JE, Chang-Claude J, Chanock S, Chen J, Chenevix-Trench G, Coll?e JM, Couch FJ, Couper D, Coveillo AD, Cox A, Czene K, D'adamo AP, Smith GD, De Vivo I, Demerath EW, Dennis J, Devilee P, Dieffenbach AK, Dunning AM, Eiriksdottir G, Eriksson JG, Fasching PA, Ferrucci L, Flesch-Janys D, Flyger H, Foroud T, Franke L, Garcia ME, Garc?a-Closas M, Geller F, de Geus EE, Giles GG, Gudbjartsson DF, Gudnason V, Gu?nel P, Guo S, Hall P, Hamann U, Haring R, Hartman CA, Heath AC, Hofman A, Hooning MJ, Hopper JL, Hu FB, Hunter DJ, Karasik D, Kiel DP, Knight JA, Kosma VM, Kutalik Z, Lai S, Lambrechts D, Lindblom A, M?gi R, Magnusson PK, Mannermaa A, Martin NG, Masson G, McArdle PF, McArdle WL, Melbye M, Michailidou K, Mihailov E, Milani L, Milne RL, Nevanlinna H, Neven P, Nohr EA, Oldehinkel AJ, Oostra BA, Palotie A, Peacock M, Pedersen NL, Peterlongo P, Peto J, Pharoah PD, Postma DS, Pouta A, Pylk?s K, Radice P, Ring S, Rivadeneira F, Robino A, Rose LM, Rudolph A, Salomaa V, Sanna S, Schlessinger D, Schmidt MK, Southey MC, Sovio U, Stampfer MJ, St?ckl D, Storniolo AM, Timpson NJ, Tyrer J, Visser JA, Vollenweider P, V?lzke H, Waeber G, Waldenberger M, Wallaschofski H, Wang Q, Willemsen G, Winqvist R, Wolffenbuttel BH, Wright MJ, , , , , , , Boomsma DI, Econs MJ, Khaw KT, Loos RJ, McCarthy MI, Montgomery GW, Rice JP, Streeten EA, Thorsteinsdottir U, van Duijn CM, Alizadeh BZ, Bergmann S, Boerwinkle E, Boyd HA, Crisponi L, Gasparini P, Gieger C, Harris TB, Ingelsson E, J?rvelin MR, Kraft P, Lawlor D, Metspalu A, Pennell CE, Ridker PM, Snieder H, S?rensen TI, Spector TD, Strachan DP, Uitterlinden AG, Wareham NJ, Widen E, Zygmunt M, Murray A, Easton DF, Stefansson K, Murabito JM, Ong KK
Nature514(7520)92-97(2014 Oct)

A genome-wide search for common SNP x SNP interactions on the risk of venous thrombosis.
Greliche N, Germain M, Lambert JC, Cohen W, Bertrand M, Dupuis AM, Letenneur L, Lathrop M, Amouyel P, Morange PE, Tr?gou?t DA
BMC medical genetics1436(2013 Mar)

Genome-wide association study of temperament in bipolar disorder reveals significant associations with three novel Loci.
Greenwood TA, Akiskal HS, Akiskal KK, , Kelsoe JR
Biological psychiatry72(4)303-10(2012 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RNA-seq evidence of biallelic expression of BRWD1 and 10 neighboring genes in at least one primary human tissue tested indicates that the expression of BRWD1 is uncoupled from the control of the maternally inherited 5mCpG imprints at the WRB differentially methylated region (DMR) in disomic controls or trisomy (Down syndrome) individuals.
Title: Trisomy 21 Alters DNA Methylation in Parent-of-Origin-Dependent and -Independent Manners.

Observational study and genome-wide association study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Genome wide association (GWA) predictors of anti-TNFalpha therapeutic responsiveness in pediatric inflammatory bowel disease.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

isolation and characterization of the gene located in the Down Syndrome critical region-2 of chromosome 21
Title: Characterisation and expression analysis of the WDR9 gene, located in the Down critical region-2 of the human chromosome 21.

The following BRWD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRWD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01477	NM_001007246.2 Latest version!	Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu93267	XM_017028375.1 Latest version!	Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu68249	XM_017028374.1 Latest version!	Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu68249	XM_011529613.1 Latest version!	Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu93266	XM_017028373.1 Latest version!	Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu09386	NM_018963.5 Latest version!	Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu22024	NM_033656.4 Latest version!	Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu68247	XM_011529611.1 Latest version!	Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu68248	XM_011529612.2 Latest version!	Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu22024	NM_033656.3	Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu09386	NM_018963.4	Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRWD1 ( NM_001007246.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001007247.1 Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu01477
Clone ID Related Accession (Same CDS sequence)	NM_001007246.2
Accession Version	NM_001007246.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	bromodomain and WD repeat-containing protein 1 isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA792365.1, BC064602.1 and AF064861.5. On Jun 8, 2011 this sequence version replaced NM_001007246.1. Transcript Variant: This variant (3) lacks multiple 3' exons and has a shorter alternate 3' sequence, as compared to variant 1. The encoded isoform C has a much shorter and distinct C-terminus, as compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC142986.1, SRR1660803.177108.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGC CGTCGTCCGC CCGACGCCCG GTGCCTCTCA TCGAGTCGGA GCTGTACTTC 
CTTATCGCCC GGTACCTATC GGCGGGCCCG TGTCGGAGAG CGGCCCAGGT GCTGGTGCAG 
GAGCTGGAGC AGTACCAGTT GTTGCCGAAG AGATTGGACT GGGAGGGCAA CGAGCACAAC 
AGGAGCTACG AGGAGTTGGT CTTGTCCAAT AAGCATGTGG CTCCTGATCA TCTTTTGCAA 
ATCTGCCAGC GCATCGGTCC TATGTTGGAT AAAGAAATTC CACCCAGTAT TTCAAGAGTC 
ACTTCTTTAC TTGGTGCAGG AAGGCAGTCT TTGCTACGTA CAGCAAAAGG TACCTTAATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007247.1
CDS	296..658
Translation	MAEPSSARRPVPLIESELYFLIARYLSAGPCRRAAQVLVQELEQYQLLPKRLDWEGNEHNRSYEELVLSNKHVAPDHLLQICQRIGPMLDKEIPPSISRVTSLLGAGRQSLLRTAKGTLI

Target ORF information:

RefSeq Version	NM_001007246.2
Organism	Homo sapiens(human)
Definition	Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007246.2

ORF Insert Sequence:

ATGGCGGAGC CGTCGTCCGC CCGACGCCCG GTGCCTCTCA TCGAGTCGGA GCTGTACTTC 
CTTATCGCCC GGTACCTATC GGCGGGCCCG TGTCGGAGAG CGGCCCAGGT GCTGGTGCAG 
GAGCTGGAGC AGTACCAGTT GTTGCCGAAG AGATTGGACT GGGAGGGCAA CGAGCACAAC 
AGGAGCTACG AGGAGTTGGT CTTGTCCAAT AAGCATGTGG CTCCTGATCA TCTTTTGCAA 
ATCTGCCAGC GCATCGGTCC TATGTTGGAT AAAGAAATTC CACCCAGTAT TTCAAGAGTC 
ACTTCTTTAC TTGGTGCAGG AAGGCAGTCT TTGCTACGTA CAGCAAAAGG TACCTTAATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRWD1 ( XM_017028375.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883864.1 Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu93267
Clone ID Related Accession (Same CDS sequence)	XM_017028375.1
Accession Version	XM_017028375.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bromodomain and WD repeat-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGC CGTCGTCCGC CCGACGCCCG GTGCCTCTCA TCGAGTCGGA GCTGTACTTC 
CTTATCGCCC GGTACCTATC GGCGGGCCCG TGTCGGAGAG CGGCCCAGGT GCTGGTGCAG 
GAGCTGGAGC AGTACCAGTT GTTGCCGAAG AGATTGGACT GGGAGGGCAA CGAGCACAAC 
AGGAGCTACG AGGAGTTGGT CTTGTCCAAT AAGCATGTGG CTCCTGATCA TCTTTTGCAA 
ATCTGCCAGC GCATCGGTCC TATGTTGGAT AAAGAAATTC CACCCAGTAT TTCAAGAGTC 
ACTTCTTTAC TTGGTGCAGG AAGGCAGTCT TTGCTACGTA CAGCAAAAGA CTGCAGGCAC 
ACAGTTTGGA AGGGCTCTGC CTTTGCTGCT CTTCATAGAG GAAGACCTCC TGAAATGCCA 
GTGAATTATG GTTCCCCACC AAATCTTGTG GAGATACATC GAGGAAAACA ACTCACAGGG 
TGTTCCACTT TTAGTACAGC ATTTCCAGGA ACTATGTATC AGCATATAAA AATGCACAGA 
AGGATTCTCG GACATCTATC TGCTGTTTAC TGTGTAGCAT TTGATAGGAC AGGACATAGA 
ATCTTTACAG GTTCAGATGA CTGTTTGGTA AAGATTTGGT CAACACATAA TGGCCGCTTG 
TTATCTACAT TAAGAGGTCA TTCTGCAGAA ATTTCAGATA TGGCAGTAAA CTATGAGAAT 
ACAATGATTG CTGCGGGGAG CTGTGATAAA ATTATTAGAG TGTGGTGCTT GAGAACTTGT 
GCCCCAGTTG CTGTGCTCCA AGGACACACA GGATCAATTA CATCTTTACA GTTTAGCCCG 
ATGGCCAAAG GCTCTCAAAG ATACATGGTT TCCACTGGTG CTGATGGGAC AGTTTGCTTT 
TGGCAATGGG ATTTAGAATC CTTAAAATTT AGCCCACGTC CCCTGAAGTT CACTGAAAAG 
CCTAGGCCAG GCGTTCAAAT GCTTTGTTCT TCTTTTAGTG TTGGTGGTAT GTTTTTAGCC 
ACAGGTAGTA CTGATCATGT AATCAGAATG TATTTTTTGG GTTTTGAAGC ACCCGAAAAA 
ATCGCAGAAC TTGAAAGCCA CACTGATAAA GTAGATAGTA TCCAATTTTG TAACAATGGT 
GATCGGTTCC TAAGTGGTAG CAGAGATGGA ACAGCACGGA TTTGGAGATT TGAGCAGTTA 
GAATGGAGGA GCATTTTATT GGATATGGCT ACCAGAATCT CAGGGGACTT ATCTTCCGAA 
GAGGAAAGGT TTATGAAACC TAAAGTAACA ATGATAGCTT GGAATCAAAA TGATAGCATT 
GTTGTCACAG CTGTGAATGA TCATGTCCTC AAAGTGTGGA ATTCTTACAC TGGACAACTG 
CTTCATAACT TAATGGGACA TGCTGATGAA GTATTTGTTC TGGAGACACA TCCCTTTGAT 
TCCAGAATTA TGTTATCTGC AGGACATGAT GGCAGCATAT TTATATGGGA TATTACAAAA 
GGTACCAAGA TGAAACATTA TTTTAATATG ATTGAAGGAC AAGGACATGG AGCTGTGTTT 
GACTGTAAGT TTTCACAGGA TGGACAGCAT TTTGCCTGTA CAGATTCTCA TGGGCACCTT 
CTGATATTTG GTTTTGGATG CAGCAAACCA TATGAAAAGA TTCCTGATCA GATGTTCTTC 
CATACTGACT ATCGACCACT TATTAGAGAT TCTAATAATT ATGTCTTAGA TGAGCAAACT 
CAGCAGGCTC CTCATCTTAT GCCTCCACCA TTCTTGGTAG ATGTAGATGG AAATCCTCAT 
CCAACCAAGT ATCAGAGATT AGTACCAGGC CGAGAAAATT CTGCAGATGA ACATTTGATT 
CCACAGCTGG GCTATGTGGC AACAAGTGAT GGAGAGGTGA TTGAACAAAT TATAAGCCTG 
CAAACCAATG ATAATGATGA ACGCAGCCCA GAATCGAGTA TTCTTGATGG AATGATAAGA 
CAGTTGCAGC AGCAGCAAGA TCAGAGAATG GGAGCAGATC AGGATACTAT TCCAAGAGGA 
CTTTCAAATG GTGAAGAAAC ACCCCGGAGA GGTTTTAGAA GGCTGAGCTT AGACATTCAG 
TCCCCTCCAA ATATTGGTCT GCGTCGTAGT GGACAAGTTG AAGGTGTTCG TCAGATGCAT 
CAAAACGCTC CACGCAGTCA GATTGCTACA GAACGTGACC TGCAGGCTTG GAAACGAAGA 
GTGGTTGTAC CAGAGGTACC ACTAGGCATA TTTAGGAAGC TGGAAGACTT CCGATTAGAG 
AAAGGTGAAG AGGAAAGAAA TCTTTATATA ATAGGAAGAA AAAGAAAGAC TCTTCAGCTC 
TCACATAAGT CGGATTCAGT GGTTTTGGTA TCACAGTCTA GACAAAGGAC ATGTAGGCGT 
AAATATCCAA ATTATGGTAG AAGAAATCGT AGCTGGCGTG AGTTATCTTC TGGAAATGAG 
TCTTCAAGCT CTGTAAGACA TGAGACTTCC TGTGATCAGA GTGAAGGTTC TGGTTCTTCA 
GAAGAGGATG AATGGAGAAG TGACAGAAAA AGTGAGAGTT ACAGCGAAAG TTCAAGTGAC 
TCTTCATCTA GATATTCCGA TTGGACAGCT GATGCGGGCA TCAATTTGCA GCCTCCTTTA 
AGAACATCAT GTCGTCGACG AATTACTCGA TTTTGTAGTA GTTCAGAAGA TGAAATATCT 
ACTGAGAATT TATCTCCTCC AAAAAGAAGA CGAAAGAGAA AGAAAGAAAA TAAGCCTAAG 
AAGGAGAATT TGCGGAGGAT GACTCCAGCA GAGCTTGCAA ATATGGAGCA TTTATATGAA 
TTTCACCCTC CAGTTTGGAT TACTGACACC ACACTTAGAA AATCTCCTTT TGTTCCTCAA 
ATGGGTGATG AGGTAATATA TTTTCGACAG GGTCATGAAG CTTATATTGA GGCTGTAAGA 
AGAAATAATA TTTATGAACT GAACCCTAAT AAGGAGCCAT GGAGAAAAAT GGATCTTAGG 
GATCAAGAAT TGGTTAAAAT AGTTGGAATA CGATATGAAG TTGGGCCCCC TACACTCTGT 
TGCCTAAAAC TAGCATTTAT AGATCCAGCA ACTGGAAAAC TTATGGACAA ATCTTTCTCT 
ATTAGGGTAA CTGGTAGAGC ACTGTGGATA GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883864.1
CDS	329..3484
Translation	MAEPSSARRPVPLIESELYFLIARYLSAGPCRRAAQVLVQELEQYQLLPKRLDWEGNEHNRSYEELVLSNKHVAPDHLLQICQRIGPMLDKEIPPSISRVTSLLGAGRQSLLRTAKDCRHTVWKGSAFAALHRGRPPEMPVNYGSPPNLVEIHRGKQLTGCSTFSTAFPGTMYQHIKMHRRILGHLSAVYCVAFDRTGHRIFTGSDDCLVKIWSTHNGRLLSTLRGHSAEISDMAVNYENTMIAAGSCDKIIRVWCLRTCAPVAVLQGHTGSITSLQFSPMAKGSQRYMVSTGADGTVCFWQWDLESLKFSPRPLKFTEKPRPGVQMLCSSFSVGGMFLATGSTDHVIRMYFLGFEAPEKIAELESHTDKVDSIQFCNNGDRFLSGSRDGTARIWRFEQLEWRSILLDMATRISGDLSSEEERFMKPKVTMIAWNQNDSIVVTAVNDHVLKVWNSYTGQLLHNLMGHADEVFVLETHPFDSRIMLSAGHDGSIFIWDITKGTKMKHYFNMIEGQGHGAVFDCKFSQDGQHFACTDSHGHLLIFGFGCSKPYEKIPDQMFFHTDYRPLIRDSNNYVLDEQTQQAPHLMPPPFLVDVDGNPHPTKYQRLVPGRENSADEHLIPQLGYVATSDGEVIEQIISLQTNDNDERSPESSILDGMIRQLQQQQDQRMGADQDTIPRGLSNGEETPRRGFRRLSLDIQSPPNIGLRRSGQVEGVRQMHQNAPRSQIATERDLQAWKRRVVVPEVPLGIFRKLEDFRLEKGEEERNLYIIGRKRKTLQLSHKSDSVVLVSQSRQRTCRRKYPNYGRRNRSWRELSSGNESSSSVRHETSCDQSEGSGSSEEDEWRSDRKSESYSESSSDSSSRYSDWTADAGINLQPPLRTSCRRRITRFCSSSEDEISTENLSPPKRRRKRKKENKPKKENLRRMTPAELANMEHLYEFHPPVWITDTTLRKSPFVPQMGDEVIYFRQGHEAYIEAVRRNNIYELNPNKEPWRKMDLRDQELVKIVGIRYEVGPPTLCCLKLAFIDPATGKLMDKSFSIRVTGRALWIA

Target ORF information:

RefSeq Version	XM_017028375.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028375.1

ORF Insert Sequence:

ATGGCGGAGC CGTCGTCCGC CCGACGCCCG GTGCCTCTCA TCGAGTCGGA GCTGTACTTC 
CTTATCGCCC GGTACCTATC GGCGGGCCCG TGTCGGAGAG CGGCCCAGGT GCTGGTGCAG 
GAGCTGGAGC AGTACCAGTT GTTGCCGAAG AGATTGGACT GGGAGGGCAA CGAGCACAAC 
AGGAGCTACG AGGAGTTGGT CTTGTCCAAT AAGCATGTGG CTCCTGATCA TCTTTTGCAA 
ATCTGCCAGC GCATCGGTCC TATGTTGGAT AAAGAAATTC CACCCAGTAT TTCAAGAGTC 
ACTTCTTTAC TTGGTGCAGG AAGGCAGTCT TTGCTACGTA CAGCAAAAGA CTGCAGGCAC 
ACAGTTTGGA AGGGCTCTGC CTTTGCTGCT CTTCATAGAG GAAGACCTCC TGAAATGCCA 
GTGAATTATG GTTCCCCACC AAATCTTGTG GAGATACATC GAGGAAAACA ACTCACAGGG 
TGTTCCACTT TTAGTACAGC ATTTCCAGGA ACTATGTATC AGCATATAAA AATGCACAGA 
AGGATTCTCG GACATCTATC TGCTGTTTAC TGTGTAGCAT TTGATAGGAC AGGACATAGA 
ATCTTTACAG GTTCAGATGA CTGTTTGGTA AAGATTTGGT CAACACATAA TGGCCGCTTG 
TTATCTACAT TAAGAGGTCA TTCTGCAGAA ATTTCAGATA TGGCAGTAAA CTATGAGAAT 
ACAATGATTG CTGCGGGGAG CTGTGATAAA ATTATTAGAG TGTGGTGCTT GAGAACTTGT 
GCCCCAGTTG CTGTGCTCCA AGGACACACA GGATCAATTA CATCTTTACA GTTTAGCCCG 
ATGGCCAAAG GCTCTCAAAG ATACATGGTT TCCACTGGTG CTGATGGGAC AGTTTGCTTT 
TGGCAATGGG ATTTAGAATC CTTAAAATTT AGCCCACGTC CCCTGAAGTT CACTGAAAAG 
CCTAGGCCAG GCGTTCAAAT GCTTTGTTCT TCTTTTAGTG TTGGTGGTAT GTTTTTAGCC 
ACAGGTAGTA CTGATCATGT AATCAGAATG TATTTTTTGG GTTTTGAAGC ACCCGAAAAA 
ATCGCAGAAC TTGAAAGCCA CACTGATAAA GTAGATAGTA TCCAATTTTG TAACAATGGT 
GATCGGTTCC TAAGTGGTAG CAGAGATGGA ACAGCACGGA TTTGGAGATT TGAGCAGTTA 
GAATGGAGGA GCATTTTATT GGATATGGCT ACCAGAATCT CAGGGGACTT ATCTTCCGAA 
GAGGAAAGGT TTATGAAACC TAAAGTAACA ATGATAGCTT GGAATCAAAA TGATAGCATT 
GTTGTCACAG CTGTGAATGA TCATGTCCTC AAAGTGTGGA ATTCTTACAC TGGACAACTG 
CTTCATAACT TAATGGGACA TGCTGATGAA GTATTTGTTC TGGAGACACA TCCCTTTGAT 
TCCAGAATTA TGTTATCTGC AGGACATGAT GGCAGCATAT TTATATGGGA TATTACAAAA 
GGTACCAAGA TGAAACATTA TTTTAATATG ATTGAAGGAC AAGGACATGG AGCTGTGTTT 
GACTGTAAGT TTTCACAGGA TGGACAGCAT TTTGCCTGTA CAGATTCTCA TGGGCACCTT 
CTGATATTTG GTTTTGGATG CAGCAAACCA TATGAAAAGA TTCCTGATCA GATGTTCTTC 
CATACTGACT ATCGACCACT TATTAGAGAT TCTAATAATT ATGTCTTAGA TGAGCAAACT 
CAGCAGGCTC CTCATCTTAT GCCTCCACCA TTCTTGGTAG ATGTAGATGG AAATCCTCAT 
CCAACCAAGT ATCAGAGATT AGTACCAGGC CGAGAAAATT CTGCAGATGA ACATTTGATT 
CCACAGCTGG GCTATGTGGC AACAAGTGAT GGAGAGGTGA TTGAACAAAT TATAAGCCTG 
CAAACCAATG ATAATGATGA ACGCAGCCCA GAATCGAGTA TTCTTGATGG AATGATAAGA 
CAGTTGCAGC AGCAGCAAGA TCAGAGAATG GGAGCAGATC AGGATACTAT TCCAAGAGGA 
CTTTCAAATG GTGAAGAAAC ACCCCGGAGA GGTTTTAGAA GGCTGAGCTT AGACATTCAG 
TCCCCTCCAA ATATTGGTCT GCGTCGTAGT GGACAAGTTG AAGGTGTTCG TCAGATGCAT 
CAAAACGCTC CACGCAGTCA GATTGCTACA GAACGTGACC TGCAGGCTTG GAAACGAAGA 
GTGGTTGTAC CAGAGGTACC ACTAGGCATA TTTAGGAAGC TGGAAGACTT CCGATTAGAG 
AAAGGTGAAG AGGAAAGAAA TCTTTATATA ATAGGAAGAA AAAGAAAGAC TCTTCAGCTC 
TCACATAAGT CGGATTCAGT GGTTTTGGTA TCACAGTCTA GACAAAGGAC ATGTAGGCGT 
AAATATCCAA ATTATGGTAG AAGAAATCGT AGCTGGCGTG AGTTATCTTC TGGAAATGAG 
TCTTCAAGCT CTGTAAGACA TGAGACTTCC TGTGATCAGA GTGAAGGTTC TGGTTCTTCA 
GAAGAGGATG AATGGAGAAG TGACAGAAAA AGTGAGAGTT ACAGCGAAAG TTCAAGTGAC 
TCTTCATCTA GATATTCCGA TTGGACAGCT GATGCGGGCA TCAATTTGCA GCCTCCTTTA 
AGAACATCAT GTCGTCGACG AATTACTCGA TTTTGTAGTA GTTCAGAAGA TGAAATATCT 
ACTGAGAATT TATCTCCTCC AAAAAGAAGA CGAAAGAGAA AGAAAGAAAA TAAGCCTAAG 
AAGGAGAATT TGCGGAGGAT GACTCCAGCA GAGCTTGCAA ATATGGAGCA TTTATATGAA 
TTTCACCCTC CAGTTTGGAT TACTGACACC ACACTTAGAA AATCTCCTTT TGTTCCTCAA 
ATGGGTGATG AGGTAATATA TTTTCGACAG GGTCATGAAG CTTATATTGA GGCTGTAAGA 
AGAAATAATA TTTATGAACT GAACCCTAAT AAGGAGCCAT GGAGAAAAAT GGATCTTAGG 
GATCAAGAAT TGGTTAAAAT AGTTGGAATA CGATATGAAG TTGGGCCCCC TACACTCTGT 
TGCCTAAAAC TAGCATTTAT AGATCCAGCA ACTGGAAAAC TTATGGACAA ATCTTTCTCT 
ATTAGGGTAA CTGGTAGAGC ACTGTGGATA GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRWD1 ( XM_017028374.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883863.1 Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu68249
Clone ID Related Accession (Same CDS sequence)	XM_017028374.1 , XM_011529613.1
Accession Version	XM_017028374.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bromodomain and WD repeat-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATC CATTTGATGG ATACTCCATC CAGGATCAAG AATTGGTTAA AATAGTTGGA 
ATACGATATG AAGTTGGGCC CCCTACACTC TGTTGCCTAA AACTAGCATT TATAGATCCA 
GCAACTGGAA AACTTATGGA CAAATCTTTC TCTATTAGAT ATCATGATAT GCCAGATGTT 
ATTGACTTTC TTGTATTGCG TCAATTTTAT GATGAAGCAA GACAGAGGAA TTGGCAGTCT 
TGTGACAGAT TCCGCTCTAT TATTGATGAT GCTTGGTGGT TTGGAACAGT GTTAAGTCAA 
GAGCCATATC AACCACAGTA TCCTGATAGT CATTTCCAGT GTTATATTGT TAGGTGGGAT 
AATACTGAAA TTGAAAAACT TAGCCCATGG GACATGGAAC CAATTCCTGA TAATGTTGAT 
CCACCTGAAG AATTAGGAGC TAGTATTTCT GTCACAACAG ATGAGCTAGA GAAATTGCTC 
TATAAACCAC AAGCTGGTGA ATGGGGTCAG AAATCAAGAG ATGAAGAATG TGATAGAATT 
ATCAGTGGTA TAGATCAACT TTTGAATCTT GATATAGCAG CAGCTTTTGC AGGCCCTGTT 
GATTTGTGTA CATACCCGAA GTACTGTACT GTAGTAGCTT ATCCAACCGA TCTTTACACA 
ATTCGAATGA GACTTGTTAA TCGATTTTAC AGGAGGCTGT CTGCGTTAGT TTGGGAAGTC 
AGATATATAG AACATAATGC CAGAACATTT AACGAACCTG AGAGTGTAAT TGCAAGATCA 
GCTAAAAAGA TAACTGACCA ACTTTTAAAA TTTATCAAGA ATCAACACTG TACAAATATC 
TCAGAACTTT CTAACACATC TGAAAATGAT GAGCAAAATG CTGAGGATTT GGATGATAGT 
GATCTTCCTA AAACATCTTC TGGAAGGAGG AGAGTCCATG ATGGGAAAAA AAGCATCAGA 
GCTACGAACT ATGTTGAAAG CAACTGGAAG AAACAGTGTA AGGAACTAGT GAACTTAATT 
TTTCAGTGTG AAGATTCTGA ACCATTTAGA CAACCTGTTG ATTTGGTTGA ATATCCAGAC 
TACAGAGATA TTATAGATAC CCCAATGGAT TTTGGAACAG TAAGGGAAAC TCTAGATGCG 
GGAAATTATG ACAGCCCTTT GGAGTTTTGC AAAGACATCC GGCTGATATT TAGCAATGCA 
AAAGCGTATA CACCAAACAA AAGATCAAAG ATTTATAGTA TGACCTTGAG ATTATCTGCC 
TTATTTGAAG AAAAAATGAA GAAAATCTCT TCTGATTTTA AAATTGGTCA AAAATTCAAT 
GAAAAACTTC GAAGAAGCCA GAGGTTCAAG CAACGGCAAA ATTGTAAAGG TGACAGTCAG 
CCTAACAAAA GTATCAGAAA CCTCAAGCCG AAGAGGTTAA AATCTCAGAC AAAAATAATT 
CCTGAGTTGG TAGGTTCTCC TACCCAGTCT ACCTCAAGTA GGACAGCTTA TCTTGGAACC 
CACAAGACAA GTGCTGGTAT CTCTTCAGGT GTTACTTCTG GTGACTCTTC AGATTCAGCA 
GAATCATCAG AAAGGAGGAA AAGAAATAGA CCTATAACAA ATGGTTCTAC ATTATCTGAA 
AGTGAAGTGG AAGATTCTTT AGCTACCTCT TTGTCATCGT CAGCTTCCAG TAGTTCTGAG 
GAAAGCAAAG AGAGTTCCAG AGCTCGTGAA TCCTCCTCAC GCAGTGGGCT ATCCAGAAGC 
AGCAATCTCA GGGTAACCAG AACTAGAGCT GCTCAAAGAA AAACTGGTCC CGTTTCATTA 
GCAAATGGAT GTGGCAGAAA AGCCACTCGA AAGAGAGTCT ATTTAAGTGA TTCTGATAAC 
AATTCATTGG AGACTGGTGA AATTCTAAAA GCCAGAGCTG GAAATAACCG AAAAGTCTTA 
AGGAAGTGTG CTGCTGTGGC TGCCAATAAA ATAAAGCTAA TGAGTGATGT AGAAGAGAAT 
TCTAGCTCTG AAAGTGTCTG TTCTGGTCGG AAGCTGCCTC ACCGCAATGC TTCTGCTGTA 
GCTAGAAAAA AGTTATTACA TAATTCTGAA GATGAACAGA GCTTAAAGTC AGAAATTGAA 
GAAGAGGAGC TAAAAGATGA AAATCAACTA TTACCAGTGT CCAGTTCTCA CACTGCCCAG 
AGCAATGTTG ATGAATCTGA AAACAGAGAC TCAGAGTCAG AAAGTGATTT GCGGGTAGCC 
CGGAAAAATT GGCATGCTAA TGGTTACAAG TCCCATACTC CAGCACCTTC AAAGACAAAA 
TTTCTTAAAA TAGAGTCTTC TGAGGAAGAC TCTAAAAGTC ATGATTCAGA TCATGCATGT 
AACAGAACTG CTGGCCCATC AACGTCTGTG CAGAAACTTA AGGCAGAGAG CATCTCAGAG 
GAAGCAGATT CTGAACCAGG AAGATCTGGT GGTAGGAAAT ACAATACATT TCACAAGAAT 
GCGAGTTTCT TTAAAAAAAC CAAGATTCTG AGTGACTCAG AAGACTCTGA ATCTGAAGAG 
CAAGATAGAG AAGATGGGAA ATGTCATAAA ATGGAAATGA ACCCAATTTC AGGAAATCTG 
AACTGTGACC CTATTGCTAT GTCCCAGTGT TCCTCAGATC ATGGATGTGA AACTGATTTA 
GATTCAGATG ATGACAAAAT AGAAAAACCA AACAATTTTA TGAAAGATTC TGCATCACAA 
GACAATGGAC TAAGCAGAAA AATTTCCAGG AAAAGGGTCT GTTCCAGTGA CTCAGACAGT 
AGTTTACAGG TGGTTAAGAA ATCATCAAAA GCCAGAACAG GTCTCCTGAG GATTACTCGA 
AGATGTGCAG CTACGGCTGC CAATAAGATC AAGCTCATGA GTGATGTAGA AGATGTCAGT 
TTAGAAAATG TGCACACTAG AAGCAAAAAT GGAAGGAAAA AACCTCTCCA TCTTGCTTGT 
ACTACAGCTA AGAAGAAATT GAGTGATTGT GAAGGAAGTG TACATTGTGA AGTACCAAGT 
GAACAGTATG CCTGTGAAGG CAAGCCACCT GATCCTGACT CCGAAGGTAG TACAAAAGTG 
CTTAGTCAGG CTCTAAATGG AGACTCAGAC TCTGAAGATA TGTTGAATTC AGAACACAAG 
CACAGGCATA CCAATATTCA CAAAATAGAT GCACCTTCTA AAAGAAAAAG TTCCTCTGTT 
ACATCTTCAG GAGAAGATTC AAAAAGTCAT ATTCCAGGGA GTGAGACTGA TAGGACATTT 
TCTTCAGAGT CAACCTTGGC ACAAAAAGCT ACTGCAGAGA ATAATTTTGA AGTGGAACTG 
AATTATGGGC TGCGCAGGTG GAATGGCAGA AGACTCAGGA CCTATGGAAA GGCTCCTTTT 
AGTAAGACAA AAGTGATTCA TGATTCACAG GAAACAGCAG AGAAGGAAGT AAAAAGGAAG 
AGATCGCATC CTGAATTGGA AAATGTGAAA ATCTCTGAAA CAACTGGGAA TTCAAAGTTT 
AGACCTGATA CTAGTTCCAA ATCATCAGAT TTGGGATCTG TAACTGAATC AGATATTGAC 
TGTACTGATA ATACAAAAAC CAAAAGGAGG AAAACGAAAG GAAAAGCAAA AGTAGTTAGA 
AAAGAATTTG TTCCTAGAGA CAGAGAACCC AATACAAAAG TGAGAACATG TATGCATAAT 
CAGAAGGATG CAGTGCAGAT GCCTAGTGAA ACTCTGAAAG CAAAAATGGT ACCTGAGAAA 
GTTCCCCGCA GATGTGCTAC TGTTGCTGCA AATAAAATAA AGATAATGAG TAATCTAAAA 
GAAACGATCT CAGGACCAGA AAATGTCTGG ATTAGGAAGA GTAGCAGAAA ACTGCCCCAT 
CGAAATGCTT CTGCTGCGGC TAAGAAAAAA TTACTGAATG TTTATAAAGA GGATGATACA 
ACCATAAATT CTGAAAGTGA AAAAGAGCTA GAAGATATTA ATAGAAAAAT GCTTTTTTTA 
AGGGGGTTCA GATCCTGGAA AGAAAATGCA CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883863.1
CDS	1898..5893
Translation	MANPFDGYSIQDQELVKIVGIRYEVGPPTLCCLKLAFIDPATGKLMDKSFSIRYHDMPDVIDFLVLRQFYDEARQRNWQSCDRFRSIIDDAWWFGTVLSQEPYQPQYPDSHFQCYIVRWDNTEIEKLSPWDMEPIPDNVDPPEELGASISVTTDELEKLLYKPQAGEWGQKSRDEECDRIISGIDQLLNLDIAAAFAGPVDLCTYPKYCTVVAYPTDLYTIRMRLVNRFYRRLSALVWEVRYIEHNARTFNEPESVIARSAKKITDQLLKFIKNQHCTNISELSNTSENDEQNAEDLDDSDLPKTSSGRRRVHDGKKSIRATNYVESNWKKQCKELVNLIFQCEDSEPFRQPVDLVEYPDYRDIIDTPMDFGTVRETLDAGNYDSPLEFCKDIRLIFSNAKAYTPNKRSKIYSMTLRLSALFEEKMKKISSDFKIGQKFNEKLRRSQRFKQRQNCKGDSQPNKSIRNLKPKRLKSQTKIIPELVGSPTQSTSSRTAYLGTHKTSAGISSGVTSGDSSDSAESSERRKRNRPITNGSTLSESEVEDSLATSLSSSASSSSEESKESSRARESSSRSGLSRSSNLRVTRTRAAQRKTGPVSLANGCGRKATRKRVYLSDSDNNSLETGEILKARAGNNRKVLRKCAAVAANKIKLMSDVEENSSSESVCSGRKLPHRNASAVARKKLLHNSEDEQSLKSEIEEEELKDENQLLPVSSSHTAQSNVDESENRDSESESDLRVARKNWHANGYKSHTPAPSKTKFLKIESSEEDSKSHDSDHACNRTAGPSTSVQKLKAESISEEADSEPGRSGGRKYNTFHKNASFFKKTKILSDSEDSESEEQDREDGKCHKMEMNPISGNLNCDPIAMSQCSSDHGCETDLDSDDDKIEKPNNFMKDSASQDNGLSRKISRKRVCSSDSDSSLQVVKKSSKARTGLLRITRRCAATAANKIKLMSDVEDVSLENVHTRSKNGRKKPLHLACTTAKKKLSDCEGSVHCEVPSEQYACEGKPPDPDSEGSTKVLSQALNGDSDSEDMLNSEHKHRHTNIHKIDAPSKRKSSSVTSSGEDSKSHIPGSETDRTFSSESTLAQKATAENNFEVELNYGLRRWNGRRLRTYGKAPFSKTKVIHDSQETAEKEVKRKRSHPELENVKISETTGNSKFRPDTSSKSSDLGSVTESDIDCTDNTKTKRRKTKGKAKVVRKEFVPRDREPNTKVRTCMHNQKDAVQMPSETLKAKMVPEKVPRRCATVAANKIKIMSNLKETISGPENVWIRKSSRKLPHRNASAAAKKKLLNVYKEDDTTINSESEKELEDINRKMLFLRGFRSWKENAQ

Target ORF information:

RefSeq Version	XM_017028374.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028374.1

ORF Insert Sequence:

ATGGCAAATC CATTTGATGG ATACTCCATC CAGGATCAAG AATTGGTTAA AATAGTTGGA 
ATACGATATG AAGTTGGGCC CCCTACACTC TGTTGCCTAA AACTAGCATT TATAGATCCA 
GCAACTGGAA AACTTATGGA CAAATCTTTC TCTATTAGAT ATCATGATAT GCCAGATGTT 
ATTGACTTTC TTGTATTGCG TCAATTTTAT GATGAAGCAA GACAGAGGAA TTGGCAGTCT 
TGTGACAGAT TCCGCTCTAT TATTGATGAT GCTTGGTGGT TTGGAACAGT GTTAAGTCAA 
GAGCCATATC AACCACAGTA TCCTGATAGT CATTTCCAGT GTTATATTGT TAGGTGGGAT 
AATACTGAAA TTGAAAAACT TAGCCCATGG GACATGGAAC CAATTCCTGA TAATGTTGAT 
CCACCTGAAG AATTAGGAGC TAGTATTTCT GTCACAACAG ATGAGCTAGA GAAATTGCTC 
TATAAACCAC AAGCTGGTGA ATGGGGTCAG AAATCAAGAG ATGAAGAATG TGATAGAATT 
ATCAGTGGTA TAGATCAACT TTTGAATCTT GATATAGCAG CAGCTTTTGC AGGCCCTGTT 
GATTTGTGTA CATACCCGAA GTACTGTACT GTAGTAGCTT ATCCAACCGA TCTTTACACA 
ATTCGAATGA GACTTGTTAA TCGATTTTAC AGGAGGCTGT CTGCGTTAGT TTGGGAAGTC 
AGATATATAG AACATAATGC CAGAACATTT AACGAACCTG AGAGTGTAAT TGCAAGATCA 
GCTAAAAAGA TAACTGACCA ACTTTTAAAA TTTATCAAGA ATCAACACTG TACAAATATC 
TCAGAACTTT CTAACACATC TGAAAATGAT GAGCAAAATG CTGAGGATTT GGATGATAGT 
GATCTTCCTA AAACATCTTC TGGAAGGAGG AGAGTCCATG ATGGGAAAAA AAGCATCAGA 
GCTACGAACT ATGTTGAAAG CAACTGGAAG AAACAGTGTA AGGAACTAGT GAACTTAATT 
TTTCAGTGTG AAGATTCTGA ACCATTTAGA CAACCTGTTG ATTTGGTTGA ATATCCAGAC 
TACAGAGATA TTATAGATAC CCCAATGGAT TTTGGAACAG TAAGGGAAAC TCTAGATGCG 
GGAAATTATG ACAGCCCTTT GGAGTTTTGC AAAGACATCC GGCTGATATT TAGCAATGCA 
AAAGCGTATA CACCAAACAA AAGATCAAAG ATTTATAGTA TGACCTTGAG ATTATCTGCC 
TTATTTGAAG AAAAAATGAA GAAAATCTCT TCTGATTTTA AAATTGGTCA AAAATTCAAT 
GAAAAACTTC GAAGAAGCCA GAGGTTCAAG CAACGGCAAA ATTGTAAAGG TGACAGTCAG 
CCTAACAAAA GTATCAGAAA CCTCAAGCCG AAGAGGTTAA AATCTCAGAC AAAAATAATT 
CCTGAGTTGG TAGGTTCTCC TACCCAGTCT ACCTCAAGTA GGACAGCTTA TCTTGGAACC 
CACAAGACAA GTGCTGGTAT CTCTTCAGGT GTTACTTCTG GTGACTCTTC AGATTCAGCA 
GAATCATCAG AAAGGAGGAA AAGAAATAGA CCTATAACAA ATGGTTCTAC ATTATCTGAA 
AGTGAAGTGG AAGATTCTTT AGCTACCTCT TTGTCATCGT CAGCTTCCAG TAGTTCTGAG 
GAAAGCAAAG AGAGTTCCAG AGCTCGTGAA TCCTCCTCAC GCAGTGGGCT ATCCAGAAGC 
AGCAATCTCA GGGTAACCAG AACTAGAGCT GCTCAAAGAA AAACTGGTCC CGTTTCATTA 
GCAAATGGAT GTGGCAGAAA AGCCACTCGA AAGAGAGTCT ATTTAAGTGA TTCTGATAAC 
AATTCATTGG AGACTGGTGA AATTCTAAAA GCCAGAGCTG GAAATAACCG AAAAGTCTTA 
AGGAAGTGTG CTGCTGTGGC TGCCAATAAA ATAAAGCTAA TGAGTGATGT AGAAGAGAAT 
TCTAGCTCTG AAAGTGTCTG TTCTGGTCGG AAGCTGCCTC ACCGCAATGC TTCTGCTGTA 
GCTAGAAAAA AGTTATTACA TAATTCTGAA GATGAACAGA GCTTAAAGTC AGAAATTGAA 
GAAGAGGAGC TAAAAGATGA AAATCAACTA TTACCAGTGT CCAGTTCTCA CACTGCCCAG 
AGCAATGTTG ATGAATCTGA AAACAGAGAC TCAGAGTCAG AAAGTGATTT GCGGGTAGCC 
CGGAAAAATT GGCATGCTAA TGGTTACAAG TCCCATACTC CAGCACCTTC AAAGACAAAA 
TTTCTTAAAA TAGAGTCTTC TGAGGAAGAC TCTAAAAGTC ATGATTCAGA TCATGCATGT 
AACAGAACTG CTGGCCCATC AACGTCTGTG CAGAAACTTA AGGCAGAGAG CATCTCAGAG 
GAAGCAGATT CTGAACCAGG AAGATCTGGT GGTAGGAAAT ACAATACATT TCACAAGAAT 
GCGAGTTTCT TTAAAAAAAC CAAGATTCTG AGTGACTCAG AAGACTCTGA ATCTGAAGAG 
CAAGATAGAG AAGATGGGAA ATGTCATAAA ATGGAAATGA ACCCAATTTC AGGAAATCTG 
AACTGTGACC CTATTGCTAT GTCCCAGTGT TCCTCAGATC ATGGATGTGA AACTGATTTA 
GATTCAGATG ATGACAAAAT AGAAAAACCA AACAATTTTA TGAAAGATTC TGCATCACAA 
GACAATGGAC TAAGCAGAAA AATTTCCAGG AAAAGGGTCT GTTCCAGTGA CTCAGACAGT 
AGTTTACAGG TGGTTAAGAA ATCATCAAAA GCCAGAACAG GTCTCCTGAG GATTACTCGA 
AGATGTGCAG CTACGGCTGC CAATAAGATC AAGCTCATGA GTGATGTAGA AGATGTCAGT 
TTAGAAAATG TGCACACTAG AAGCAAAAAT GGAAGGAAAA AACCTCTCCA TCTTGCTTGT 
ACTACAGCTA AGAAGAAATT GAGTGATTGT GAAGGAAGTG TACATTGTGA AGTACCAAGT 
GAACAGTATG CCTGTGAAGG CAAGCCACCT GATCCTGACT CCGAAGGTAG TACAAAAGTG 
CTTAGTCAGG CTCTAAATGG AGACTCAGAC TCTGAAGATA TGTTGAATTC AGAACACAAG 
CACAGGCATA CCAATATTCA CAAAATAGAT GCACCTTCTA AAAGAAAAAG TTCCTCTGTT 
ACATCTTCAG GAGAAGATTC AAAAAGTCAT ATTCCAGGGA GTGAGACTGA TAGGACATTT 
TCTTCAGAGT CAACCTTGGC ACAAAAAGCT ACTGCAGAGA ATAATTTTGA AGTGGAACTG 
AATTATGGGC TGCGCAGGTG GAATGGCAGA AGACTCAGGA CCTATGGAAA GGCTCCTTTT 
AGTAAGACAA AAGTGATTCA TGATTCACAG GAAACAGCAG AGAAGGAAGT AAAAAGGAAG 
AGATCGCATC CTGAATTGGA AAATGTGAAA ATCTCTGAAA CAACTGGGAA TTCAAAGTTT 
AGACCTGATA CTAGTTCCAA ATCATCAGAT TTGGGATCTG TAACTGAATC AGATATTGAC 
TGTACTGATA ATACAAAAAC CAAAAGGAGG AAAACGAAAG GAAAAGCAAA AGTAGTTAGA 
AAAGAATTTG TTCCTAGAGA CAGAGAACCC AATACAAAAG TGAGAACATG TATGCATAAT 
CAGAAGGATG CAGTGCAGAT GCCTAGTGAA ACTCTGAAAG CAAAAATGGT ACCTGAGAAA 
GTTCCCCGCA GATGTGCTAC TGTTGCTGCA AATAAAATAA AGATAATGAG TAATCTAAAA 
GAAACGATCT CAGGACCAGA AAATGTCTGG ATTAGGAAGA GTAGCAGAAA ACTGCCCCAT 
CGAAATGCTT CTGCTGCGGC TAAGAAAAAA TTACTGAATG TTTATAAAGA GGATGATACA 
ACCATAAATT CTGAAAGTGA AAAAGAGCTA GAAGATATTA ATAGAAAAAT GCTTTTTTTA 
AGGGGGTTCA GATCCTGGAA AGAAAATGCA CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRWD1 ( XM_011529613.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527915.1 Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu68249
Clone ID Related Accession (Same CDS sequence)	XM_017028374.1 , XM_011529613.1
Accession Version	XM_011529613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bromodomain and WD repeat-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATC CATTTGATGG ATACTCCATC CAGGATCAAG AATTGGTTAA AATAGTTGGA 
ATACGATATG AAGTTGGGCC CCCTACACTC TGTTGCCTAA AACTAGCATT TATAGATCCA 
GCAACTGGAA AACTTATGGA CAAATCTTTC TCTATTAGAT ATCATGATAT GCCAGATGTT 
ATTGACTTTC TTGTATTGCG TCAATTTTAT GATGAAGCAA GACAGAGGAA TTGGCAGTCT 
TGTGACAGAT TCCGCTCTAT TATTGATGAT GCTTGGTGGT TTGGAACAGT GTTAAGTCAA 
GAGCCATATC AACCACAGTA TCCTGATAGT CATTTCCAGT GTTATATTGT TAGGTGGGAT 
AATACTGAAA TTGAAAAACT TAGCCCATGG GACATGGAAC CAATTCCTGA TAATGTTGAT 
CCACCTGAAG AATTAGGAGC TAGTATTTCT GTCACAACAG ATGAGCTAGA GAAATTGCTC 
TATAAACCAC AAGCTGGTGA ATGGGGTCAG AAATCAAGAG ATGAAGAATG TGATAGAATT 
ATCAGTGGTA TAGATCAACT TTTGAATCTT GATATAGCAG CAGCTTTTGC AGGCCCTGTT 
GATTTGTGTA CATACCCGAA GTACTGTACT GTAGTAGCTT ATCCAACCGA TCTTTACACA 
ATTCGAATGA GACTTGTTAA TCGATTTTAC AGGAGGCTGT CTGCGTTAGT TTGGGAAGTC 
AGATATATAG AACATAATGC CAGAACATTT AACGAACCTG AGAGTGTAAT TGCAAGATCA 
GCTAAAAAGA TAACTGACCA ACTTTTAAAA TTTATCAAGA ATCAACACTG TACAAATATC 
TCAGAACTTT CTAACACATC TGAAAATGAT GAGCAAAATG CTGAGGATTT GGATGATAGT 
GATCTTCCTA AAACATCTTC TGGAAGGAGG AGAGTCCATG ATGGGAAAAA AAGCATCAGA 
GCTACGAACT ATGTTGAAAG CAACTGGAAG AAACAGTGTA AGGAACTAGT GAACTTAATT 
TTTCAGTGTG AAGATTCTGA ACCATTTAGA CAACCTGTTG ATTTGGTTGA ATATCCAGAC 
TACAGAGATA TTATAGATAC CCCAATGGAT TTTGGAACAG TAAGGGAAAC TCTAGATGCG 
GGAAATTATG ACAGCCCTTT GGAGTTTTGC AAAGACATCC GGCTGATATT TAGCAATGCA 
AAAGCGTATA CACCAAACAA AAGATCAAAG ATTTATAGTA TGACCTTGAG ATTATCTGCC 
TTATTTGAAG AAAAAATGAA GAAAATCTCT TCTGATTTTA AAATTGGTCA AAAATTCAAT 
GAAAAACTTC GAAGAAGCCA GAGGTTCAAG CAACGGCAAA ATTGTAAAGG TGACAGTCAG 
CCTAACAAAA GTATCAGAAA CCTCAAGCCG AAGAGGTTAA AATCTCAGAC AAAAATAATT 
CCTGAGTTGG TAGGTTCTCC TACCCAGTCT ACCTCAAGTA GGACAGCTTA TCTTGGAACC 
CACAAGACAA GTGCTGGTAT CTCTTCAGGT GTTACTTCTG GTGACTCTTC AGATTCAGCA 
GAATCATCAG AAAGGAGGAA AAGAAATAGA CCTATAACAA ATGGTTCTAC ATTATCTGAA 
AGTGAAGTGG AAGATTCTTT AGCTACCTCT TTGTCATCGT CAGCTTCCAG TAGTTCTGAG 
GAAAGCAAAG AGAGTTCCAG AGCTCGTGAA TCCTCCTCAC GCAGTGGGCT ATCCAGAAGC 
AGCAATCTCA GGGTAACCAG AACTAGAGCT GCTCAAAGAA AAACTGGTCC CGTTTCATTA 
GCAAATGGAT GTGGCAGAAA AGCCACTCGA AAGAGAGTCT ATTTAAGTGA TTCTGATAAC 
AATTCATTGG AGACTGGTGA AATTCTAAAA GCCAGAGCTG GAAATAACCG AAAAGTCTTA 
AGGAAGTGTG CTGCTGTGGC TGCCAATAAA ATAAAGCTAA TGAGTGATGT AGAAGAGAAT 
TCTAGCTCTG AAAGTGTCTG TTCTGGTCGG AAGCTGCCTC ACCGCAATGC TTCTGCTGTA 
GCTAGAAAAA AGTTATTACA TAATTCTGAA GATGAACAGA GCTTAAAGTC AGAAATTGAA 
GAAGAGGAGC TAAAAGATGA AAATCAACTA TTACCAGTGT CCAGTTCTCA CACTGCCCAG 
AGCAATGTTG ATGAATCTGA AAACAGAGAC TCAGAGTCAG AAAGTGATTT GCGGGTAGCC 
CGGAAAAATT GGCATGCTAA TGGTTACAAG TCCCATACTC CAGCACCTTC AAAGACAAAA 
TTTCTTAAAA TAGAGTCTTC TGAGGAAGAC TCTAAAAGTC ATGATTCAGA TCATGCATGT 
AACAGAACTG CTGGCCCATC AACGTCTGTG CAGAAACTTA AGGCAGAGAG CATCTCAGAG 
GAAGCAGATT CTGAACCAGG AAGATCTGGT GGTAGGAAAT ACAATACATT TCACAAGAAT 
GCGAGTTTCT TTAAAAAAAC CAAGATTCTG AGTGACTCAG AAGACTCTGA ATCTGAAGAG 
CAAGATAGAG AAGATGGGAA ATGTCATAAA ATGGAAATGA ACCCAATTTC AGGAAATCTG 
AACTGTGACC CTATTGCTAT GTCCCAGTGT TCCTCAGATC ATGGATGTGA AACTGATTTA 
GATTCAGATG ATGACAAAAT AGAAAAACCA AACAATTTTA TGAAAGATTC TGCATCACAA 
GACAATGGAC TAAGCAGAAA AATTTCCAGG AAAAGGGTCT GTTCCAGTGA CTCAGACAGT 
AGTTTACAGG TGGTTAAGAA ATCATCAAAA GCCAGAACAG GTCTCCTGAG GATTACTCGA 
AGATGTGCAG CTACGGCTGC CAATAAGATC AAGCTCATGA GTGATGTAGA AGATGTCAGT 
TTAGAAAATG TGCACACTAG AAGCAAAAAT GGAAGGAAAA AACCTCTCCA TCTTGCTTGT 
ACTACAGCTA AGAAGAAATT GAGTGATTGT GAAGGAAGTG TACATTGTGA AGTACCAAGT 
GAACAGTATG CCTGTGAAGG CAAGCCACCT GATCCTGACT CCGAAGGTAG TACAAAAGTG 
CTTAGTCAGG CTCTAAATGG AGACTCAGAC TCTGAAGATA TGTTGAATTC AGAACACAAG 
CACAGGCATA CCAATATTCA CAAAATAGAT GCACCTTCTA AAAGAAAAAG TTCCTCTGTT 
ACATCTTCAG GAGAAGATTC AAAAAGTCAT ATTCCAGGGA GTGAGACTGA TAGGACATTT 
TCTTCAGAGT CAACCTTGGC ACAAAAAGCT ACTGCAGAGA ATAATTTTGA AGTGGAACTG 
AATTATGGGC TGCGCAGGTG GAATGGCAGA AGACTCAGGA CCTATGGAAA GGCTCCTTTT 
AGTAAGACAA AAGTGATTCA TGATTCACAG GAAACAGCAG AGAAGGAAGT AAAAAGGAAG 
AGATCGCATC CTGAATTGGA AAATGTGAAA ATCTCTGAAA CAACTGGGAA TTCAAAGTTT 
AGACCTGATA CTAGTTCCAA ATCATCAGAT TTGGGATCTG TAACTGAATC AGATATTGAC 
TGTACTGATA ATACAAAAAC CAAAAGGAGG AAAACGAAAG GAAAAGCAAA AGTAGTTAGA 
AAAGAATTTG TTCCTAGAGA CAGAGAACCC AATACAAAAG TGAGAACATG TATGCATAAT 
CAGAAGGATG CAGTGCAGAT GCCTAGTGAA ACTCTGAAAG CAAAAATGGT ACCTGAGAAA 
GTTCCCCGCA GATGTGCTAC TGTTGCTGCA AATAAAATAA AGATAATGAG TAATCTAAAA 
GAAACGATCT CAGGACCAGA AAATGTCTGG ATTAGGAAGA GTAGCAGAAA ACTGCCCCAT 
CGAAATGCTT CTGCTGCGGC TAAGAAAAAA TTACTGAATG TTTATAAAGA GGATGATACA 
ACCATAAATT CTGAAAGTGA AAAAGAGCTA GAAGATATTA ATAGAAAAAT GCTTTTTTTA 
AGGGGGTTCA GATCCTGGAA AGAAAATGCA CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527915.1
CDS	258..4253
Translation	MANPFDGYSIQDQELVKIVGIRYEVGPPTLCCLKLAFIDPATGKLMDKSFSIRYHDMPDVIDFLVLRQFYDEARQRNWQSCDRFRSIIDDAWWFGTVLSQEPYQPQYPDSHFQCYIVRWDNTEIEKLSPWDMEPIPDNVDPPEELGASISVTTDELEKLLYKPQAGEWGQKSRDEECDRIISGIDQLLNLDIAAAFAGPVDLCTYPKYCTVVAYPTDLYTIRMRLVNRFYRRLSALVWEVRYIEHNARTFNEPESVIARSAKKITDQLLKFIKNQHCTNISELSNTSENDEQNAEDLDDSDLPKTSSGRRRVHDGKKSIRATNYVESNWKKQCKELVNLIFQCEDSEPFRQPVDLVEYPDYRDIIDTPMDFGTVRETLDAGNYDSPLEFCKDIRLIFSNAKAYTPNKRSKIYSMTLRLSALFEEKMKKISSDFKIGQKFNEKLRRSQRFKQRQNCKGDSQPNKSIRNLKPKRLKSQTKIIPELVGSPTQSTSSRTAYLGTHKTSAGISSGVTSGDSSDSAESSERRKRNRPITNGSTLSESEVEDSLATSLSSSASSSSEESKESSRARESSSRSGLSRSSNLRVTRTRAAQRKTGPVSLANGCGRKATRKRVYLSDSDNNSLETGEILKARAGNNRKVLRKCAAVAANKIKLMSDVEENSSSESVCSGRKLPHRNASAVARKKLLHNSEDEQSLKSEIEEEELKDENQLLPVSSSHTAQSNVDESENRDSESESDLRVARKNWHANGYKSHTPAPSKTKFLKIESSEEDSKSHDSDHACNRTAGPSTSVQKLKAESISEEADSEPGRSGGRKYNTFHKNASFFKKTKILSDSEDSESEEQDREDGKCHKMEMNPISGNLNCDPIAMSQCSSDHGCETDLDSDDDKIEKPNNFMKDSASQDNGLSRKISRKRVCSSDSDSSLQVVKKSSKARTGLLRITRRCAATAANKIKLMSDVEDVSLENVHTRSKNGRKKPLHLACTTAKKKLSDCEGSVHCEVPSEQYACEGKPPDPDSEGSTKVLSQALNGDSDSEDMLNSEHKHRHTNIHKIDAPSKRKSSSVTSSGEDSKSHIPGSETDRTFSSESTLAQKATAENNFEVELNYGLRRWNGRRLRTYGKAPFSKTKVIHDSQETAEKEVKRKRSHPELENVKISETTGNSKFRPDTSSKSSDLGSVTESDIDCTDNTKTKRRKTKGKAKVVRKEFVPRDREPNTKVRTCMHNQKDAVQMPSETLKAKMVPEKVPRRCATVAANKIKIMSNLKETISGPENVWIRKSSRKLPHRNASAAAKKKLLNVYKEDDTTINSESEKELEDINRKMLFLRGFRSWKENAQ

Target ORF information:

RefSeq Version	XM_011529613.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529613.1

ORF Insert Sequence:

ATGGCAAATC CATTTGATGG ATACTCCATC CAGGATCAAG AATTGGTTAA AATAGTTGGA 
ATACGATATG AAGTTGGGCC CCCTACACTC TGTTGCCTAA AACTAGCATT TATAGATCCA 
GCAACTGGAA AACTTATGGA CAAATCTTTC TCTATTAGAT ATCATGATAT GCCAGATGTT 
ATTGACTTTC TTGTATTGCG TCAATTTTAT GATGAAGCAA GACAGAGGAA TTGGCAGTCT 
TGTGACAGAT TCCGCTCTAT TATTGATGAT GCTTGGTGGT TTGGAACAGT GTTAAGTCAA 
GAGCCATATC AACCACAGTA TCCTGATAGT CATTTCCAGT GTTATATTGT TAGGTGGGAT 
AATACTGAAA TTGAAAAACT TAGCCCATGG GACATGGAAC CAATTCCTGA TAATGTTGAT 
CCACCTGAAG AATTAGGAGC TAGTATTTCT GTCACAACAG ATGAGCTAGA GAAATTGCTC 
TATAAACCAC AAGCTGGTGA ATGGGGTCAG AAATCAAGAG ATGAAGAATG TGATAGAATT 
ATCAGTGGTA TAGATCAACT TTTGAATCTT GATATAGCAG CAGCTTTTGC AGGCCCTGTT 
GATTTGTGTA CATACCCGAA GTACTGTACT GTAGTAGCTT ATCCAACCGA TCTTTACACA 
ATTCGAATGA GACTTGTTAA TCGATTTTAC AGGAGGCTGT CTGCGTTAGT TTGGGAAGTC 
AGATATATAG AACATAATGC CAGAACATTT AACGAACCTG AGAGTGTAAT TGCAAGATCA 
GCTAAAAAGA TAACTGACCA ACTTTTAAAA TTTATCAAGA ATCAACACTG TACAAATATC 
TCAGAACTTT CTAACACATC TGAAAATGAT GAGCAAAATG CTGAGGATTT GGATGATAGT 
GATCTTCCTA AAACATCTTC TGGAAGGAGG AGAGTCCATG ATGGGAAAAA AAGCATCAGA 
GCTACGAACT ATGTTGAAAG CAACTGGAAG AAACAGTGTA AGGAACTAGT GAACTTAATT 
TTTCAGTGTG AAGATTCTGA ACCATTTAGA CAACCTGTTG ATTTGGTTGA ATATCCAGAC 
TACAGAGATA TTATAGATAC CCCAATGGAT TTTGGAACAG TAAGGGAAAC TCTAGATGCG 
GGAAATTATG ACAGCCCTTT GGAGTTTTGC AAAGACATCC GGCTGATATT TAGCAATGCA 
AAAGCGTATA CACCAAACAA AAGATCAAAG ATTTATAGTA TGACCTTGAG ATTATCTGCC 
TTATTTGAAG AAAAAATGAA GAAAATCTCT TCTGATTTTA AAATTGGTCA AAAATTCAAT 
GAAAAACTTC GAAGAAGCCA GAGGTTCAAG CAACGGCAAA ATTGTAAAGG TGACAGTCAG 
CCTAACAAAA GTATCAGAAA CCTCAAGCCG AAGAGGTTAA AATCTCAGAC AAAAATAATT 
CCTGAGTTGG TAGGTTCTCC TACCCAGTCT ACCTCAAGTA GGACAGCTTA TCTTGGAACC 
CACAAGACAA GTGCTGGTAT CTCTTCAGGT GTTACTTCTG GTGACTCTTC AGATTCAGCA 
GAATCATCAG AAAGGAGGAA AAGAAATAGA CCTATAACAA ATGGTTCTAC ATTATCTGAA 
AGTGAAGTGG AAGATTCTTT AGCTACCTCT TTGTCATCGT CAGCTTCCAG TAGTTCTGAG 
GAAAGCAAAG AGAGTTCCAG AGCTCGTGAA TCCTCCTCAC GCAGTGGGCT ATCCAGAAGC 
AGCAATCTCA GGGTAACCAG AACTAGAGCT GCTCAAAGAA AAACTGGTCC CGTTTCATTA 
GCAAATGGAT GTGGCAGAAA AGCCACTCGA AAGAGAGTCT ATTTAAGTGA TTCTGATAAC 
AATTCATTGG AGACTGGTGA AATTCTAAAA GCCAGAGCTG GAAATAACCG AAAAGTCTTA 
AGGAAGTGTG CTGCTGTGGC TGCCAATAAA ATAAAGCTAA TGAGTGATGT AGAAGAGAAT 
TCTAGCTCTG AAAGTGTCTG TTCTGGTCGG AAGCTGCCTC ACCGCAATGC TTCTGCTGTA 
GCTAGAAAAA AGTTATTACA TAATTCTGAA GATGAACAGA GCTTAAAGTC AGAAATTGAA 
GAAGAGGAGC TAAAAGATGA AAATCAACTA TTACCAGTGT CCAGTTCTCA CACTGCCCAG 
AGCAATGTTG ATGAATCTGA AAACAGAGAC TCAGAGTCAG AAAGTGATTT GCGGGTAGCC 
CGGAAAAATT GGCATGCTAA TGGTTACAAG TCCCATACTC CAGCACCTTC AAAGACAAAA 
TTTCTTAAAA TAGAGTCTTC TGAGGAAGAC TCTAAAAGTC ATGATTCAGA TCATGCATGT 
AACAGAACTG CTGGCCCATC AACGTCTGTG CAGAAACTTA AGGCAGAGAG CATCTCAGAG 
GAAGCAGATT CTGAACCAGG AAGATCTGGT GGTAGGAAAT ACAATACATT TCACAAGAAT 
GCGAGTTTCT TTAAAAAAAC CAAGATTCTG AGTGACTCAG AAGACTCTGA ATCTGAAGAG 
CAAGATAGAG AAGATGGGAA ATGTCATAAA ATGGAAATGA ACCCAATTTC AGGAAATCTG 
AACTGTGACC CTATTGCTAT GTCCCAGTGT TCCTCAGATC ATGGATGTGA AACTGATTTA 
GATTCAGATG ATGACAAAAT AGAAAAACCA AACAATTTTA TGAAAGATTC TGCATCACAA 
GACAATGGAC TAAGCAGAAA AATTTCCAGG AAAAGGGTCT GTTCCAGTGA CTCAGACAGT 
AGTTTACAGG TGGTTAAGAA ATCATCAAAA GCCAGAACAG GTCTCCTGAG GATTACTCGA 
AGATGTGCAG CTACGGCTGC CAATAAGATC AAGCTCATGA GTGATGTAGA AGATGTCAGT 
TTAGAAAATG TGCACACTAG AAGCAAAAAT GGAAGGAAAA AACCTCTCCA TCTTGCTTGT 
ACTACAGCTA AGAAGAAATT GAGTGATTGT GAAGGAAGTG TACATTGTGA AGTACCAAGT 
GAACAGTATG CCTGTGAAGG CAAGCCACCT GATCCTGACT CCGAAGGTAG TACAAAAGTG 
CTTAGTCAGG CTCTAAATGG AGACTCAGAC TCTGAAGATA TGTTGAATTC AGAACACAAG 
CACAGGCATA CCAATATTCA CAAAATAGAT GCACCTTCTA AAAGAAAAAG TTCCTCTGTT 
ACATCTTCAG GAGAAGATTC AAAAAGTCAT ATTCCAGGGA GTGAGACTGA TAGGACATTT 
TCTTCAGAGT CAACCTTGGC ACAAAAAGCT ACTGCAGAGA ATAATTTTGA AGTGGAACTG 
AATTATGGGC TGCGCAGGTG GAATGGCAGA AGACTCAGGA CCTATGGAAA GGCTCCTTTT 
AGTAAGACAA AAGTGATTCA TGATTCACAG GAAACAGCAG AGAAGGAAGT AAAAAGGAAG 
AGATCGCATC CTGAATTGGA AAATGTGAAA ATCTCTGAAA CAACTGGGAA TTCAAAGTTT 
AGACCTGATA CTAGTTCCAA ATCATCAGAT TTGGGATCTG TAACTGAATC AGATATTGAC 
TGTACTGATA ATACAAAAAC CAAAAGGAGG AAAACGAAAG GAAAAGCAAA AGTAGTTAGA 
AAAGAATTTG TTCCTAGAGA CAGAGAACCC AATACAAAAG TGAGAACATG TATGCATAAT 
CAGAAGGATG CAGTGCAGAT GCCTAGTGAA ACTCTGAAAG CAAAAATGGT ACCTGAGAAA 
GTTCCCCGCA GATGTGCTAC TGTTGCTGCA AATAAAATAA AGATAATGAG TAATCTAAAA 
GAAACGATCT CAGGACCAGA AAATGTCTGG ATTAGGAAGA GTAGCAGAAA ACTGCCCCAT 
CGAAATGCTT CTGCTGCGGC TAAGAAAAAA TTACTGAATG TTTATAAAGA GGATGATACA 
ACCATAAATT CTGAAAGTGA AAAAGAGCTA GAAGATATTA ATAGAAAAAT GCTTTTTTTA 
AGGGGGTTCA GATCCTGGAA AGAAAATGCA CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRWD1 ( XM_017028373.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883862.1 Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant X2, mRNA.

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CloneID	OHu93266
Clone ID Related Accession (Same CDS sequence)	XM_017028373.1
Accession Version	XM_017028373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bromodomain and WD repeat-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGATA AAGAAATTCC ACCCAGTATT TCAAGAGTCA CTTCTTTACT TGGTGCAGGA 
AGGCAGTCTT TGCTACGTAC AGCAAAAGAC TGCAGGCACA CAGTTTGGAA GGGCTCTGCC 
TTTGCTGCTC TTCATAGAGG AAGACCTCCT GAAATGCCAG TGAATTATGG TTCCCCACCA 
AATCTTGTGG AGATACATCG AGGAAAACAA CTCACAGGGT GTTCCACTTT TAGTACAGCA 
TTTCCAGGAA CTATGTATCA GCATATAAAA ATGCACAGAA GGATTCTCGG ACATCTATCT 
GCTGTTTACT GTGTAGCATT TGATAGGACA GGACATAGAA TCTTTACAGG TTCAGATGAC 
TGTTTGGTAA AGATTTGGTC AACACATAAT GGCCGCTTGT TATCTACATT AAGAGGTCAT 
TCTGCAGAAA TTTCAGATAT GGCAGTAAAC TATGAGAATA CAATGATTGC TGCGGGGAGC 
TGTGATAAAA TTATTAGAGT GTGGTGCTTG AGAACTTGTG CCCCAGTTGC TGTGCTCCAA 
GGACACACAG GATCAATTAC ATCTTTACAG TTTAGCCCGA TGGCCAAAGG CTCTCAAAGA 
TACATGGTTT CCACTGGTGC TGATGGGACA GTTTGCTTTT GGCAATGGGA TTTAGAATCC 
TTAAAATTTA GCCCACGTCC CCTGAAGTTC ACTGAAAAGC CTAGGCCAGG CGTTCAAATG 
CTTTGTTCTT CTTTTAGTGT TGGTGGTATG TTTTTAGCCA CAGGTAGTAC TGATCATGTA 
ATCAGAATGT ATTTTTTGGG TTTTGAAGCA CCCGAAAAAA TCGCAGAACT TGAAAGCCAC 
ACTGATAAAG TAGATAGTAT CCAATTTTGT AACAATGGTG ATCGGTTCCT AAGTGGTAGC 
AGAGATGGAA CAGCACGGAT TTGGAGATTT GAGCAGTTAG AATGGAGGAG CATTTTATTG 
GATATGGCTA CCAGAATCTC AGGGGACTTA TCTTCCGAAG AGGAAAGGTT TATGAAACCT 
AAAGTAACAA TGATAGCTTG GAATCAAAAT GATAGCATTG TTGTCACAGC TGTGAATGAT 
CATGTCCTCA AAGTGTGGAA TTCTTACACT GGACAACTGC TTCATAACTT AATGGGACAT 
GCTGATGAAG TATTTGTTCT GGAGACACAT CCCTTTGATT CCAGAATTAT GTTATCTGCA 
GGACATGATG GCAGCATATT TATATGGGAT ATTACAAAAG GTACCAAGAT GAAACATTAT 
TTTAATATGA TTGAAGGACA AGGACATGGA GCTGTGTTTG ACTGTAAGTT TTCACAGGAT 
GGACAGCATT TTGCCTGTAC AGATTCTCAT GGGCACCTTC TGATATTTGG TTTTGGATGC 
AGCAAACCAT ATGAAAAGAT TCCTGATCAG ATGTTCTTCC ATACTGACTA TCGACCACTT 
ATTAGAGATT CTAATAATTA TGTCTTAGAT GAGCAAACTC AGCAGGCTCC TCATCTTATG 
CCTCCACCAT TCTTGGTAGA TGTAGATGGA AATCCTCATC CAACCAAGTA TCAGAGATTA 
GTACCAGGCC GAGAAAATTC TGCAGATGAA CATTTGATTC CACAGCTGGG CTATGTGGCA 
ACAAGTGATG GAGAGGTGAT TGAACAAATT ATAAGCCTGC AAACCAATGA TAATGATGAA 
CGCAGCCCAG AATCGAGTAT TCTTGATGGA ATGATAAGAC AGTTGCAGCA GCAGCAAGAT 
CAGAGAATGG GAGCAGATCA GGATACTATT CCAAGAGGAC TTTCAAATGG TGAAGAAACA 
CCCCGGAGAG GTTTTAGAAG GCTGAGCTTA GACATTCAGT CCCCTCCAAA TATTGGTCTG 
CGTCGTAGTG GACAAGTTGA AGGTGTTCGT CAGATGCATC AAAACGCTCC ACGCAGTCAG 
ATTGCTACAG AACGTGACCT GCAGGCTTGG AAACGAAGAG TGGTTGTACC AGAGGTACCA 
CTAGGCATAT TTAGGAAGCT GGAAGACTTC CGATTAGAGA AAGGTGAAGA GGAAAGAAAT 
CTTTATATAA TAGGAAGAAA AAGAAAGACT CTTCAGCTCT CACATAAGTC GGATTCAGTG 
GTTTTGGTAT CACAGTCTAG ACAAAGGACA TGTAGGCGTA AATATCCAAA TTATGGTAGA 
AGAAATCGTA GCTGGCGTGA GTTATCTTCT GGAAATGAGT CTTCAAGCTC TGTAAGACAT 
GAGACTTCCT GTGATCAGAG TGAAGGTTCT GGTTCTTCAG AAGAGGATGA ATGGAGAAGT 
GACAGAAAAA GTGAGAGTTA CAGCGAAAGT TCAAGTGACT CTTCATCTAG ATATTCCGAT 
TGGACAGCTG ATGCGGGCAT CAATTTGCAG CCTCCTTTAA GAACATCATG TCGTCGACGA 
ATTACTCGAT TTTGTAGTAG TTCAGAAGAT GAAATATCTA CTGAGAATTT ATCTCCTCCA 
AAAAGAAGAC GAAAGAGAAA GAAAGAAAAT AAGCCTAAGA AGGAGAATTT GCGGAGGATG 
ACTCCAGCAG AGCTTGCAAA TATGGAGCAT TTATATGAAT TTCACCCTCC AGTTTGGATT 
ACTGACACCA CACTTAGAAA ATCTCCTTTT GTTCCTCAAA TGGGTGATGA GGTAATATAT 
TTTCGACAGG GTCATGAAGC TTATATTGAG GCTGTAAGAA GAAATAATAT TTATGAACTG 
AACCCTAATA AGGAGCCATG GAGAAAAATG GATCTTAGGG ATCAAGAATT GGTTAAAATA 
GTTGGAATAC GATATGAAGT TGGGCCCCCT ACACTCTGTT GCCTAAAACT AGCATTTATA 
GATCCAGCAA CTGGAAAACT TATGGACAAA TCTTTCTCTA TTAGATATCA TGATATGCCA 
GATGTTATTG ACTTTCTTGT ATTGCGTCAA TTTTATGATG AAGCAAGACA GAGGAATTGG 
CAGTCTTGTG ACAGATTCCG CTCTATTATT GATGATGCTT GGTGGTTTGG AACAGTGTTA 
AGTCAAGAGC CATATCAACC ACAGTATCCT GATAGTCATT TCCAGTGTTA TATTGTTAGG 
TGGGATAATA CTGAAATTGA AAAACTTAGC CCATGGGACA TGGAACCAAT TCCTGATAAT 
GTTGATCCAC CTGAAGAATT AGGAGCTAGT ATTTCTGTCA CAACAGATGA GCTAGAGAAA 
TTGCTCTATA AACCACAAGC TGGTGAATGG GGTCAGAAAT CAAGAGATGA AGAATGTGAT 
AGAATTATCA GTGGTATAGA TCAACTTTTG AATCTTGATA TAGCAGCAGC TTTTGCAGGC 
CCTGTTGATT TGTGTACATA CCCGAAGTAC TGTACTGTAG TAGCTTATCC AACCGATCTT 
TACACAATTC GAATGAGACT TGTTAATCGA TTTTACAGGA GGCTGTCTGC GTTAGTTTGG 
GAAGTCAGAT ATATAGAACA TAATGCCAGA ACATTTAACG AACCTGAGAG TGTAATTGCA 
AGATCAGCTA AAAAGATAAC TGACCAACTT TTAAAATTTA TCAAGAATCA ACACTGTACA 
AATATCTCAG AACTTTCTAA CACATCTGAA AATGATGAGC AAAATGCTGA GGATTTGGAT 
GATAGTGATC TTCCTAAAAC ATCTTCTGGA AGGAGGAGAG TCCATGATGG GAAAAAAAGC 
ATCAGAGCTA CGAACTATGT TGAAAGCAAC TGGAAGAAAC AGTGTAAGGA ACTAGTGAAC 
TTAATTTTTC AGTGTGAAGA TTCTGAACCA TTTAGACAAC CTGTTGATTT GGTTGAATAT 
CCAGACTACA GAGATATTAT AGATACCCCA ATGGATTTTG GAACAGTAAG GGAAACTCTA 
GATGCGGGAA ATTATGACAG CCCTTTGGAG TTTTGCAAAG ACATCCGGCT GATATTTAGC 
AATGCAAAAG CGTATACACC AAACAAAAGA TCAAAGATTT ATAGTATGAC CTTGAGATTA 
TCTGCCTTAT TTGAAGAAAA AATGAAGAAA ATCTCTTCTG ATTTTAAAAT TGGTCAAAAA 
TTCAATGAAA AACTTCGAAG AAGCCAGAGG TTCAAGCAAC GGCAAAATTG TAAAGGTGAC 
AGTCAGCCTA ACAAAAGTAT CAGAAACCTC AAGCCGAAGA GGTTAAAATC TCAGACAAAA 
ATAATTCCTG AGTTGGTAGG TTCTCCTACC CAGTCTACCT CAAGTAGGAC AGCTTATCTT 
GGAACCCACA AGACAAGTGC TGGTATCTCT TCAGGTGTTA CTTCTGGTGA CTCTTCAGAT 
TCAGCAGAAT CATCAGAAAG GAGGAAAAGA AATAGACCTA TAACAAATGG TTCTACATTA 
TCTGAAAGTG AAGTGGAAGA TTCTTTAGCT ACCTCTTTGT CATCGTCAGC TTCCAGTAGT 
TCTGAGGAAA GCAAAGAGAG TTCCAGAGCT CGTGAATCCT CCTCACGCAG TGGGCTATCC 
AGAAGCAGCA ATCTCAGGGT AACCAGAACT AGAGCTGCTC AAAGAAAAAC TGGTCCCGTT 
TCATTAGCAA ATGGATGTGG CAGAAAAGCC ACTCGAAAGA GAGTCTATTT AAGTGATTCT 
GATAACAATT CATTGGAGAC TGGTGAAATT CTAAAAGCCA GAGCTGGAAA TAACCGAAAA 
GTCTTAAGGA AGTGTGCTGC TGTGGCTGCC AATAAAATAA AGCTAATGAG TGATGTAGAA 
GAGAATTCTA GCTCTGAAAG TGTCTGTTCT GGTCGGAAGC TGCCTCACCG CAATGCTTCT 
GCTGTAGCTA GAAAAAAGTT ATTACATAAT TCTGAAGATG AACAGAGCTT AAAGTCAGAA 
ATTGAAGAAG AGGAGCTAAA AGATGAAAAT CAACTATTAC CAGTGTCCAG TTCTCACACT 
GCCCAGAGCA ATGTTGATGA ATCTGAAAAC AGAGACTCAG AGTCAGAAAG TGATTTGCGG 
GTAGCCCGGA AAAATTGGCA TGCTAATGGT TACAAGTCCC ATACTCCAGC ACCTTCAAAG 
ACAAAATTTC TTAAAATAGA GTCTTCTGAG GAAGACTCTA AAAGTCATGA TTCAGATCAT 
GCATGTAACA GAACTGCTGG CCCATCAACG TCTGTGCAGA AACTTAAGGC AGAGAGCATC 
TCAGAGGAAG CAGATTCTGA ACCAGGAAGA TCTGGTGGTA GGAAATACAA TACATTTCAC 
AAGAATGCGA GTTTCTTTAA AAAAACCAAG ATTCTGAGTG ACTCAGAAGA CTCTGAATCT 
GAAGAGCAAG ATAGAGAAGA TGGGAAATGT CATAAAATGG AAATGAACCC AATTTCAGGA 
AATCTGAACT GTGACCCTAT TGCTATGTCC CAGTGTTCCT CAGATCATGG ATGTGAAACT 
GATTTAGATT CAGATGATGA CAAAATAGAA AAACCAAACA ATTTTATGAA AGATTCTGCA 
TCACAAGACA ATGGACTAAG CAGAAAAATT TCCAGGAAAA GGGTCTGTTC CAGTGACTCA 
GACAGTAGTT TACAGGTGGT TAAGAAATCA TCAAAAGCCA GAACAGGTCT CCTGAGGATT 
ACTCGAAGAT GTGCAGCTAC GGCTGCCAAT AAGATCAAGC TCATGAGTGA TGTAGAAGAT 
GTCAGTTTAG AAAATGTGCA CACTAGAAGC AAAAATGGAA GGAAAAAACC TCTCCATCTT 
GCTTGTACTA CAGCTAAGAA GAAATTGAGT GATTGTGAAG GAAGTGTACA TTGTGAAGTA 
CCAAGTGAAC AGTATGCCTG TGAAGGCAAG CCACCTGATC CTGACTCCGA AGGTAGTACA 
AAAGTGCTTA GTCAGGCTCT AAATGGAGAC TCAGACTCTG AAGATATGTT GAATTCAGAA 
CACAAGCACA GGCATACCAA TATTCACAAA ATAGATGCAC CTTCTAAAAG AAAAAGTTCC 
TCTGTTACAT CTTCAGGAGA AGATTCAAAA AGTCATATTC CAGGGAGTGA GACTGATAGG 
ACATTTTCTT CAGAGTCAAC CTTGGCACAA AAAGCTACTG CAGAGAATAA TTTTGAAGTG 
GAACTGAATT ATGGGCTGCG CAGGTGGAAT GGCAGAAGAC TCAGGACCTA TGGAAAGGCT 
CCTTTTAGTA AGACAAAAGT GATTCATGAT TCACAGGAAA CAGCAGAGAA GGAAGTAAAA 
AGGAAGAGAT CGCATCCTGA ATTGGAAAAT GTGAAAATCT CTGAAACAAC TGGGAATTCA 
AAGTTTAGAC CTGATACTAG TTCCAAATCA TCAGATTTGG GATCTGTAAC TGAATCAGAT 
ATTGACTGTA CTGATAATAC AAAAACCAAA AGGAGGAAAA CGAAAGGAAA AGCAAAAGTA 
GTTAGAAAAG AATTTGTTCC TAGAGACAGA GAACCCAATA CAAAAGTGAG AACATGTATG 
CATAATCAGA AGGATGCAGT GCAGATGCCT AGTGAAACTC TGAAAGCAAA AATGGTACCT 
GAGAAAGTTC CCCGCAGATG TGCTACTGTT GCTGCAAATA AAATAAAGAT AATGAGTAAT 
CTAAAAGAAA CGATCTCAGG ACCAGAAAAT GTCTGGATTA GGAAGAGTAG CAGAAAACTG 
CCCCATCGAA ATGCTTCTGC TGCGGCTAAG AAAAAATTAC TGAATGTTTA TAAAGAGGAT 
GATACAACCA TAAATTCTGA AAGTGAAAAA GAGCTAGAAG ATATTAATAG AAAAATGCTT 
TTTTTAAGGG GGTTCAGATC CTGGAAAGAA AATGCACAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883862.1
CDS	362..7063
Translation	MLDKEIPPSISRVTSLLGAGRQSLLRTAKDCRHTVWKGSAFAALHRGRPPEMPVNYGSPPNLVEIHRGKQLTGCSTFSTAFPGTMYQHIKMHRRILGHLSAVYCVAFDRTGHRIFTGSDDCLVKIWSTHNGRLLSTLRGHSAEISDMAVNYENTMIAAGSCDKIIRVWCLRTCAPVAVLQGHTGSITSLQFSPMAKGSQRYMVSTGADGTVCFWQWDLESLKFSPRPLKFTEKPRPGVQMLCSSFSVGGMFLATGSTDHVIRMYFLGFEAPEKIAELESHTDKVDSIQFCNNGDRFLSGSRDGTARIWRFEQLEWRSILLDMATRISGDLSSEEERFMKPKVTMIAWNQNDSIVVTAVNDHVLKVWNSYTGQLLHNLMGHADEVFVLETHPFDSRIMLSAGHDGSIFIWDITKGTKMKHYFNMIEGQGHGAVFDCKFSQDGQHFACTDSHGHLLIFGFGCSKPYEKIPDQMFFHTDYRPLIRDSNNYVLDEQTQQAPHLMPPPFLVDVDGNPHPTKYQRLVPGRENSADEHLIPQLGYVATSDGEVIEQIISLQTNDNDERSPESSILDGMIRQLQQQQDQRMGADQDTIPRGLSNGEETPRRGFRRLSLDIQSPPNIGLRRSGQVEGVRQMHQNAPRSQIATERDLQAWKRRVVVPEVPLGIFRKLEDFRLEKGEEERNLYIIGRKRKTLQLSHKSDSVVLVSQSRQRTCRRKYPNYGRRNRSWRELSSGNESSSSVRHETSCDQSEGSGSSEEDEWRSDRKSESYSESSSDSSSRYSDWTADAGINLQPPLRTSCRRRITRFCSSSEDEISTENLSPPKRRRKRKKENKPKKENLRRMTPAELANMEHLYEFHPPVWITDTTLRKSPFVPQMGDEVIYFRQGHEAYIEAVRRNNIYELNPNKEPWRKMDLRDQELVKIVGIRYEVGPPTLCCLKLAFIDPATGKLMDKSFSIRYHDMPDVIDFLVLRQFYDEARQRNWQSCDRFRSIIDDAWWFGTVLSQEPYQPQYPDSHFQCYIVRWDNTEIEKLSPWDMEPIPDNVDPPEELGASISVTTDELEKLLYKPQAGEWGQKSRDEECDRIISGIDQLLNLDIAAAFAGPVDLCTYPKYCTVVAYPTDLYTIRMRLVNRFYRRLSALVWEVRYIEHNARTFNEPESVIARSAKKITDQLLKFIKNQHCTNISELSNTSENDEQNAEDLDDSDLPKTSSGRRRVHDGKKSIRATNYVESNWKKQCKELVNLIFQCEDSEPFRQPVDLVEYPDYRDIIDTPMDFGTVRETLDAGNYDSPLEFCKDIRLIFSNAKAYTPNKRSKIYSMTLRLSALFEEKMKKISSDFKIGQKFNEKLRRSQRFKQRQNCKGDSQPNKSIRNLKPKRLKSQTKIIPELVGSPTQSTSSRTAYLGTHKTSAGISSGVTSGDSSDSAESSERRKRNRPITNGSTLSESEVEDSLATSLSSSASSSSEESKESSRARESSSRSGLSRSSNLRVTRTRAAQRKTGPVSLANGCGRKATRKRVYLSDSDNNSLETGEILKARAGNNRKVLRKCAAVAANKIKLMSDVEENSSSESVCSGRKLPHRNASAVARKKLLHNSEDEQSLKSEIEEEELKDENQLLPVSSSHTAQSNVDESENRDSESESDLRVARKNWHANGYKSHTPAPSKTKFLKIESSEEDSKSHDSDHACNRTAGPSTSVQKLKAESISEEADSEPGRSGGRKYNTFHKNASFFKKTKILSDSEDSESEEQDREDGKCHKMEMNPISGNLNCDPIAMSQCSSDHGCETDLDSDDDKIEKPNNFMKDSASQDNGLSRKISRKRVCSSDSDSSLQVVKKSSKARTGLLRITRRCAATAANKIKLMSDVEDVSLENVHTRSKNGRKKPLHLACTTAKKKLSDCEGSVHCEVPSEQYACEGKPPDPDSEGSTKVLSQALNGDSDSEDMLNSEHKHRHTNIHKIDAPSKRKSSSVTSSGEDSKSHIPGSETDRTFSSESTLAQKATAENNFEVELNYGLRRWNGRRLRTYGKAPFSKTKVIHDSQETAEKEVKRKRSHPELENVKISETTGNSKFRPDTSSKSSDLGSVTESDIDCTDNTKTKRRKTKGKAKVVRKEFVPRDREPNTKVRTCMHNQKDAVQMPSETLKAKMVPEKVPRRCATVAANKIKIMSNLKETISGPENVWIRKSSRKLPHRNASAAAKKKLLNVYKEDDTTINSESEKELEDINRKMLFLRGFRSWKENAQ

Target ORF information:

RefSeq Version	XM_017028373.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028373.1

ORF Insert Sequence:

ATGTTGGATA AAGAAATTCC ACCCAGTATT TCAAGAGTCA CTTCTTTACT TGGTGCAGGA 
AGGCAGTCTT TGCTACGTAC AGCAAAAGAC TGCAGGCACA CAGTTTGGAA GGGCTCTGCC 
TTTGCTGCTC TTCATAGAGG AAGACCTCCT GAAATGCCAG TGAATTATGG TTCCCCACCA 
AATCTTGTGG AGATACATCG AGGAAAACAA CTCACAGGGT GTTCCACTTT TAGTACAGCA 
TTTCCAGGAA CTATGTATCA GCATATAAAA ATGCACAGAA GGATTCTCGG ACATCTATCT 
GCTGTTTACT GTGTAGCATT TGATAGGACA GGACATAGAA TCTTTACAGG TTCAGATGAC 
TGTTTGGTAA AGATTTGGTC AACACATAAT GGCCGCTTGT TATCTACATT AAGAGGTCAT 
TCTGCAGAAA TTTCAGATAT GGCAGTAAAC TATGAGAATA CAATGATTGC TGCGGGGAGC 
TGTGATAAAA TTATTAGAGT GTGGTGCTTG AGAACTTGTG CCCCAGTTGC TGTGCTCCAA 
GGACACACAG GATCAATTAC ATCTTTACAG TTTAGCCCGA TGGCCAAAGG CTCTCAAAGA 
TACATGGTTT CCACTGGTGC TGATGGGACA GTTTGCTTTT GGCAATGGGA TTTAGAATCC 
TTAAAATTTA GCCCACGTCC CCTGAAGTTC ACTGAAAAGC CTAGGCCAGG CGTTCAAATG 
CTTTGTTCTT CTTTTAGTGT TGGTGGTATG TTTTTAGCCA CAGGTAGTAC TGATCATGTA 
ATCAGAATGT ATTTTTTGGG TTTTGAAGCA CCCGAAAAAA TCGCAGAACT TGAAAGCCAC 
ACTGATAAAG TAGATAGTAT CCAATTTTGT AACAATGGTG ATCGGTTCCT AAGTGGTAGC 
AGAGATGGAA CAGCACGGAT TTGGAGATTT GAGCAGTTAG AATGGAGGAG CATTTTATTG 
GATATGGCTA CCAGAATCTC AGGGGACTTA TCTTCCGAAG AGGAAAGGTT TATGAAACCT 
AAAGTAACAA TGATAGCTTG GAATCAAAAT GATAGCATTG TTGTCACAGC TGTGAATGAT 
CATGTCCTCA AAGTGTGGAA TTCTTACACT GGACAACTGC TTCATAACTT AATGGGACAT 
GCTGATGAAG TATTTGTTCT GGAGACACAT CCCTTTGATT CCAGAATTAT GTTATCTGCA 
GGACATGATG GCAGCATATT TATATGGGAT ATTACAAAAG GTACCAAGAT GAAACATTAT 
TTTAATATGA TTGAAGGACA AGGACATGGA GCTGTGTTTG ACTGTAAGTT TTCACAGGAT 
GGACAGCATT TTGCCTGTAC AGATTCTCAT GGGCACCTTC TGATATTTGG TTTTGGATGC 
AGCAAACCAT ATGAAAAGAT TCCTGATCAG ATGTTCTTCC ATACTGACTA TCGACCACTT 
ATTAGAGATT CTAATAATTA TGTCTTAGAT GAGCAAACTC AGCAGGCTCC TCATCTTATG 
CCTCCACCAT TCTTGGTAGA TGTAGATGGA AATCCTCATC CAACCAAGTA TCAGAGATTA 
GTACCAGGCC GAGAAAATTC TGCAGATGAA CATTTGATTC CACAGCTGGG CTATGTGGCA 
ACAAGTGATG GAGAGGTGAT TGAACAAATT ATAAGCCTGC AAACCAATGA TAATGATGAA 
CGCAGCCCAG AATCGAGTAT TCTTGATGGA ATGATAAGAC AGTTGCAGCA GCAGCAAGAT 
CAGAGAATGG GAGCAGATCA GGATACTATT CCAAGAGGAC TTTCAAATGG TGAAGAAACA 
CCCCGGAGAG GTTTTAGAAG GCTGAGCTTA GACATTCAGT CCCCTCCAAA TATTGGTCTG 
CGTCGTAGTG GACAAGTTGA AGGTGTTCGT CAGATGCATC AAAACGCTCC ACGCAGTCAG 
ATTGCTACAG AACGTGACCT GCAGGCTTGG AAACGAAGAG TGGTTGTACC AGAGGTACCA 
CTAGGCATAT TTAGGAAGCT GGAAGACTTC CGATTAGAGA AAGGTGAAGA GGAAAGAAAT 
CTTTATATAA TAGGAAGAAA AAGAAAGACT CTTCAGCTCT CACATAAGTC GGATTCAGTG 
GTTTTGGTAT CACAGTCTAG ACAAAGGACA TGTAGGCGTA AATATCCAAA TTATGGTAGA 
AGAAATCGTA GCTGGCGTGA GTTATCTTCT GGAAATGAGT CTTCAAGCTC TGTAAGACAT 
GAGACTTCCT GTGATCAGAG TGAAGGTTCT GGTTCTTCAG AAGAGGATGA ATGGAGAAGT 
GACAGAAAAA GTGAGAGTTA CAGCGAAAGT TCAAGTGACT CTTCATCTAG ATATTCCGAT 
TGGACAGCTG ATGCGGGCAT CAATTTGCAG CCTCCTTTAA GAACATCATG TCGTCGACGA 
ATTACTCGAT TTTGTAGTAG TTCAGAAGAT GAAATATCTA CTGAGAATTT ATCTCCTCCA 
AAAAGAAGAC GAAAGAGAAA GAAAGAAAAT AAGCCTAAGA AGGAGAATTT GCGGAGGATG 
ACTCCAGCAG AGCTTGCAAA TATGGAGCAT TTATATGAAT TTCACCCTCC AGTTTGGATT 
ACTGACACCA CACTTAGAAA ATCTCCTTTT GTTCCTCAAA TGGGTGATGA GGTAATATAT 
TTTCGACAGG GTCATGAAGC TTATATTGAG GCTGTAAGAA GAAATAATAT TTATGAACTG 
AACCCTAATA AGGAGCCATG GAGAAAAATG GATCTTAGGG ATCAAGAATT GGTTAAAATA 
GTTGGAATAC GATATGAAGT TGGGCCCCCT ACACTCTGTT GCCTAAAACT AGCATTTATA 
GATCCAGCAA CTGGAAAACT TATGGACAAA TCTTTCTCTA TTAGATATCA TGATATGCCA 
GATGTTATTG ACTTTCTTGT ATTGCGTCAA TTTTATGATG AAGCAAGACA GAGGAATTGG 
CAGTCTTGTG ACAGATTCCG CTCTATTATT GATGATGCTT GGTGGTTTGG AACAGTGTTA 
AGTCAAGAGC CATATCAACC ACAGTATCCT GATAGTCATT TCCAGTGTTA TATTGTTAGG 
TGGGATAATA CTGAAATTGA AAAACTTAGC CCATGGGACA TGGAACCAAT TCCTGATAAT 
GTTGATCCAC CTGAAGAATT AGGAGCTAGT ATTTCTGTCA CAACAGATGA GCTAGAGAAA 
TTGCTCTATA AACCACAAGC TGGTGAATGG GGTCAGAAAT CAAGAGATGA AGAATGTGAT 
AGAATTATCA GTGGTATAGA TCAACTTTTG AATCTTGATA TAGCAGCAGC TTTTGCAGGC 
CCTGTTGATT TGTGTACATA CCCGAAGTAC TGTACTGTAG TAGCTTATCC AACCGATCTT 
TACACAATTC GAATGAGACT TGTTAATCGA TTTTACAGGA GGCTGTCTGC GTTAGTTTGG 
GAAGTCAGAT ATATAGAACA TAATGCCAGA ACATTTAACG AACCTGAGAG TGTAATTGCA 
AGATCAGCTA AAAAGATAAC TGACCAACTT TTAAAATTTA TCAAGAATCA ACACTGTACA 
AATATCTCAG AACTTTCTAA CACATCTGAA AATGATGAGC AAAATGCTGA GGATTTGGAT 
GATAGTGATC TTCCTAAAAC ATCTTCTGGA AGGAGGAGAG TCCATGATGG GAAAAAAAGC 
ATCAGAGCTA CGAACTATGT TGAAAGCAAC TGGAAGAAAC AGTGTAAGGA ACTAGTGAAC 
TTAATTTTTC AGTGTGAAGA TTCTGAACCA TTTAGACAAC CTGTTGATTT GGTTGAATAT 
CCAGACTACA GAGATATTAT AGATACCCCA ATGGATTTTG GAACAGTAAG GGAAACTCTA 
GATGCGGGAA ATTATGACAG CCCTTTGGAG TTTTGCAAAG ACATCCGGCT GATATTTAGC 
AATGCAAAAG CGTATACACC AAACAAAAGA TCAAAGATTT ATAGTATGAC CTTGAGATTA 
TCTGCCTTAT TTGAAGAAAA AATGAAGAAA ATCTCTTCTG ATTTTAAAAT TGGTCAAAAA 
TTCAATGAAA AACTTCGAAG AAGCCAGAGG TTCAAGCAAC GGCAAAATTG TAAAGGTGAC 
AGTCAGCCTA ACAAAAGTAT CAGAAACCTC AAGCCGAAGA GGTTAAAATC TCAGACAAAA 
ATAATTCCTG AGTTGGTAGG TTCTCCTACC CAGTCTACCT CAAGTAGGAC AGCTTATCTT 
GGAACCCACA AGACAAGTGC TGGTATCTCT TCAGGTGTTA CTTCTGGTGA CTCTTCAGAT 
TCAGCAGAAT CATCAGAAAG GAGGAAAAGA AATAGACCTA TAACAAATGG TTCTACATTA 
TCTGAAAGTG AAGTGGAAGA TTCTTTAGCT ACCTCTTTGT CATCGTCAGC TTCCAGTAGT 
TCTGAGGAAA GCAAAGAGAG TTCCAGAGCT CGTGAATCCT CCTCACGCAG TGGGCTATCC 
AGAAGCAGCA ATCTCAGGGT AACCAGAACT AGAGCTGCTC AAAGAAAAAC TGGTCCCGTT 
TCATTAGCAA ATGGATGTGG CAGAAAAGCC ACTCGAAAGA GAGTCTATTT AAGTGATTCT 
GATAACAATT CATTGGAGAC TGGTGAAATT CTAAAAGCCA GAGCTGGAAA TAACCGAAAA 
GTCTTAAGGA AGTGTGCTGC TGTGGCTGCC AATAAAATAA AGCTAATGAG TGATGTAGAA 
GAGAATTCTA GCTCTGAAAG TGTCTGTTCT GGTCGGAAGC TGCCTCACCG CAATGCTTCT 
GCTGTAGCTA GAAAAAAGTT ATTACATAAT TCTGAAGATG AACAGAGCTT AAAGTCAGAA 
ATTGAAGAAG AGGAGCTAAA AGATGAAAAT CAACTATTAC CAGTGTCCAG TTCTCACACT 
GCCCAGAGCA ATGTTGATGA ATCTGAAAAC AGAGACTCAG AGTCAGAAAG TGATTTGCGG 
GTAGCCCGGA AAAATTGGCA TGCTAATGGT TACAAGTCCC ATACTCCAGC ACCTTCAAAG 
ACAAAATTTC TTAAAATAGA GTCTTCTGAG GAAGACTCTA AAAGTCATGA TTCAGATCAT 
GCATGTAACA GAACTGCTGG CCCATCAACG TCTGTGCAGA AACTTAAGGC AGAGAGCATC 
TCAGAGGAAG CAGATTCTGA ACCAGGAAGA TCTGGTGGTA GGAAATACAA TACATTTCAC 
AAGAATGCGA GTTTCTTTAA AAAAACCAAG ATTCTGAGTG ACTCAGAAGA CTCTGAATCT 
GAAGAGCAAG ATAGAGAAGA TGGGAAATGT CATAAAATGG AAATGAACCC AATTTCAGGA 
AATCTGAACT GTGACCCTAT TGCTATGTCC CAGTGTTCCT CAGATCATGG ATGTGAAACT 
GATTTAGATT CAGATGATGA CAAAATAGAA AAACCAAACA ATTTTATGAA AGATTCTGCA 
TCACAAGACA ATGGACTAAG CAGAAAAATT TCCAGGAAAA GGGTCTGTTC CAGTGACTCA 
GACAGTAGTT TACAGGTGGT TAAGAAATCA TCAAAAGCCA GAACAGGTCT CCTGAGGATT 
ACTCGAAGAT GTGCAGCTAC GGCTGCCAAT AAGATCAAGC TCATGAGTGA TGTAGAAGAT 
GTCAGTTTAG AAAATGTGCA CACTAGAAGC AAAAATGGAA GGAAAAAACC TCTCCATCTT 
GCTTGTACTA CAGCTAAGAA GAAATTGAGT GATTGTGAAG GAAGTGTACA TTGTGAAGTA 
CCAAGTGAAC AGTATGCCTG TGAAGGCAAG CCACCTGATC CTGACTCCGA AGGTAGTACA 
AAAGTGCTTA GTCAGGCTCT AAATGGAGAC TCAGACTCTG AAGATATGTT GAATTCAGAA 
CACAAGCACA GGCATACCAA TATTCACAAA ATAGATGCAC CTTCTAAAAG AAAAAGTTCC 
TCTGTTACAT CTTCAGGAGA AGATTCAAAA AGTCATATTC CAGGGAGTGA GACTGATAGG 
ACATTTTCTT CAGAGTCAAC CTTGGCACAA AAAGCTACTG CAGAGAATAA TTTTGAAGTG 
GAACTGAATT ATGGGCTGCG CAGGTGGAAT GGCAGAAGAC TCAGGACCTA TGGAAAGGCT 
CCTTTTAGTA AGACAAAAGT GATTCATGAT TCACAGGAAA CAGCAGAGAA GGAAGTAAAA 
AGGAAGAGAT CGCATCCTGA ATTGGAAAAT GTGAAAATCT CTGAAACAAC TGGGAATTCA 
AAGTTTAGAC CTGATACTAG TTCCAAATCA TCAGATTTGG GATCTGTAAC TGAATCAGAT 
ATTGACTGTA CTGATAATAC AAAAACCAAA AGGAGGAAAA CGAAAGGAAA AGCAAAAGTA 
GTTAGAAAAG AATTTGTTCC TAGAGACAGA GAACCCAATA CAAAAGTGAG AACATGTATG 
CATAATCAGA AGGATGCAGT GCAGATGCCT AGTGAAACTC TGAAAGCAAA AATGGTACCT 
GAGAAAGTTC CCCGCAGATG TGCTACTGTT GCTGCAAATA AAATAAAGAT AATGAGTAAT 
CTAAAAGAAA CGATCTCAGG ACCAGAAAAT GTCTGGATTA GGAAGAGTAG CAGAAAACTG 
CCCCATCGAA ATGCTTCTGC TGCGGCTAAG AAAAAATTAC TGAATGTTTA TAAAGAGGAT 
GATACAACCA TAAATTCTGA AAGTGAAAAA GAGCTAGAAG ATATTAATAG AAAAATGCTT 
TTTTTAAGGG GGTTCAGATC CTGGAAAGAA AATGCACAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRWD1 ( NM_018963.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_061836.2 Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant 1, mRNA.

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CloneID	OHu09386
Clone ID Related Accession (Same CDS sequence)	NM_018963.4 , NM_018963.5
Accession Version	NM_018963.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	bromodomain and WD repeat-containing protein 1 isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA792365.1, AJ292465.1, AK125906.1, AJ238214.2, AU139205.1, BQ930702.1, BP397411.1 and AA807344.1. On Jun 8, 2011 this sequence version replaced NM_018963.3. Transcript Variant: This variant (1) encodes the longest isoform (A). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ292465.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGC CGTCGTCCGC CCGACGCCCG GTGCCTCTCA TCGAGTCGGA GCTGTACTTC 
CTTATCGCCC GGTACCTATC GGCGGGCCCG TGTCGGAGAG CGGCCCAGGT GCTGGTGCAG 
GAGCTGGAGC AGTACCAGTT GTTGCCGAAG AGATTGGACT GGGAGGGCAA CGAGCACAAC 
AGGAGCTACG AGGAGTTGGT CTTGTCCAAT AAGCATGTGG CTCCTGATCA TCTTTTGCAA 
ATCTGCCAGC GCATCGGTCC TATGTTGGAT AAAGAAATTC CACCCAGTAT TTCAAGAGTC 
ACTTCTTTAC TTGGTGCAGG AAGGCAGTCT TTGCTACGTA CAGCAAAAGA CTGCAGGCAC 
ACAGTTTGGA AGGGCTCTGC CTTTGCTGCT CTTCATAGAG GAAGACCTCC TGAAATGCCA 
GTGAATTATG GTTCCCCACC AAATCTTGTG GAGATACATC GAGGAAAACA ACTCACAGGG 
TGTTCCACTT TTAGTACAGC ATTTCCAGGA ACTATGTATC AGCATATAAA AATGCACAGA 
AGGATTCTCG GACATCTATC TGCTGTTTAC TGTGTAGCAT TTGATAGGAC AGGACATAGA 
ATCTTTACAG GTTCAGATGA CTGTTTGGTA AAGATTTGGT CAACACATAA TGGCCGCTTG 
TTATCTACAT TAAGAGGTCA TTCTGCAGAA ATTTCAGATA TGGCAGTAAA CTATGAGAAT 
ACAATGATTG CTGCGGGGAG CTGTGATAAA ATTATTAGAG TGTGGTGCTT GAGAACTTGT 
GCCCCAGTTG CTGTGCTCCA AGGACACACA GGATCAATTA CATCTTTACA GTTTAGCCCG 
ATGGCCAAAG GCTCTCAAAG ATACATGGTT TCCACTGGTG CTGATGGGAC AGTTTGCTTT 
TGGCAATGGG ATTTAGAATC CTTAAAATTT AGCCCACGTC CCCTGAAGTT CACTGAAAAG 
CCTAGGCCAG GCGTTCAAAT GCTTTGTTCT TCTTTTAGTG TTGGTGGTAT GTTTTTAGCC 
ACAGGTAGTA CTGATCATGT AATCAGAATG TATTTTTTGG GTTTTGAAGC ACCCGAAAAA 
ATCGCAGAAC TTGAAAGCCA CACTGATAAA GTAGATAGTA TCCAATTTTG TAACAATGGT 
GATCGGTTCC TAAGTGGTAG CAGAGATGGA ACAGCACGGA TTTGGAGATT TGAGCAGTTA 
GAATGGAGGA GCATTTTATT GGATATGGCT ACCAGAATCT CAGGGGACTT ATCTTCCGAA 
GAGGAAAGGT TTATGAAACC TAAAGTAACA ATGATAGCTT GGAATCAAAA TGATAGCATT 
GTTGTCACAG CTGTGAATGA TCATGTCCTC AAAGTGTGGA ATTCTTACAC TGGACAACTG 
CTTCATAACT TAATGGGACA TGCTGATGAA GTATTTGTTC TGGAGACACA TCCCTTTGAT 
TCCAGAATTA TGTTATCTGC AGGACATGAT GGCAGCATAT TTATATGGGA TATTACAAAA 
GGTACCAAGA TGAAACATTA TTTTAATATG ATTGAAGGAC AAGGACATGG AGCTGTGTTT 
GACTGTAAGT TTTCACAGGA TGGACAGCAT TTTGCCTGTA CAGATTCTCA TGGGCACCTT 
CTGATATTTG GTTTTGGATG CAGCAAACCA TATGAAAAGA TTCCTGATCA GATGTTCTTC 
CATACTGACT ATCGACCACT TATTAGAGAT TCTAATAATT ATGTCTTAGA TGAGCAAACT 
CAGCAGGCTC CTCATCTTAT GCCTCCACCA TTCTTGGTAG ATGTAGATGG AAATCCTCAT 
CCAACCAAGT ATCAGAGATT AGTACCAGGC CGAGAAAATT CTGCAGATGA ACATTTGATT 
CCACAGCTGG GCTATGTGGC AACAAGTGAT GGAGAGGTGA TTGAACAAAT TATAAGCCTG 
CAAACCAATG ATAATGATGA ACGCAGCCCA GAATCGAGTA TTCTTGATGG AATGATAAGA 
CAGTTGCAGC AGCAGCAAGA TCAGAGAATG GGAGCAGATC AGGATACTAT TCCAAGAGGA 
CTTTCAAATG GTGAAGAAAC ACCCCGGAGA GGTTTTAGAA GGCTGAGCTT AGACATTCAG 
TCCCCTCCAA ATATTGGTCT GCGTCGTAGT GGACAAGTTG AAGGTGTTCG TCAGATGCAT 
CAAAACGCTC CACGCAGTCA GATTGCTACA GAACGTGACC TGCAGGCTTG GAAACGAAGA 
GTGGTTGTAC CAGAGGTACC ACTAGGCATA TTTAGGAAGC TGGAAGACTT CCGATTAGAG 
AAAGGTGAAG AGGAAAGAAA TCTTTATATA ATAGGAAGAA AAAGAAAGAC TCTTCAGCTC 
TCACATAAGT CGGATTCAGT GGTTTTGGTA TCACAGTCTA GACAAAGGAC ATGTAGGCGT 
AAATATCCAA ATTATGGTAG AAGAAATCGT AGCTGGCGTG AGTTATCTTC TGGAAATGAG 
TCTTCAAGCT CTGTAAGACA TGAGACTTCC TGTGATCAGA GTGAAGGTTC TGGTTCTTCA 
GAAGAGGATG AATGGAGAAG TGACAGAAAA AGTGAGAGTT ACAGCGAAAG TTCAAGTGAC 
TCTTCATCTA GATATTCCGA TTGGACAGCT GATGCGGGCA TCAATTTGCA GCCTCCTTTA 
AGAACATCAT GTCGTCGACG AATTACTCGA TTTTGTAGTA GTTCAGAAGA TGAAATATCT 
ACTGAGAATT TATCTCCTCC AAAAAGAAGA CGAAAGAGAA AGAAAGAAAA TAAGCCTAAG 
AAGGAGAATT TGCGGAGGAT GACTCCAGCA GAGCTTGCAA ATATGGAGCA TTTATATGAA 
TTTCACCCTC CAGTTTGGAT TACTGACACC ACACTTAGAA AATCTCCTTT TGTTCCTCAA 
ATGGGTGATG AGGTAATATA TTTTCGACAG GGTCATGAAG CTTATATTGA GGCTGTAAGA 
AGAAATAATA TTTATGAACT GAACCCTAAT AAGGAGCCAT GGAGAAAAAT GGATCTTAGG 
GATCAAGAAT TGGTTAAAAT AGTTGGAATA CGATATGAAG TTGGGCCCCC TACACTCTGT 
TGCCTAAAAC TAGCATTTAT AGATCCAGCA ACTGGAAAAC TTATGGACAA ATCTTTCTCT 
ATTAGATATC ATGATATGCC AGATGTTATT GACTTTCTTG TATTGCGTCA ATTTTATGAT 
GAAGCAAGAC AGAGGAATTG GCAGTCTTGT GACAGATTCC GCTCTATTAT TGATGATGCT 
TGGTGGTTTG GAACAGTGTT AAGTCAAGAG CCATATCAAC CACAGTATCC TGATAGTCAT 
TTCCAGTGTT ATATTGTTAG GTGGGATAAT ACTGAAATTG AAAAACTTAG CCCATGGGAC 
ATGGAACCAA TTCCTGATAA TGTTGATCCA CCTGAAGAAT TAGGAGCTAG TATTTCTGTC 
ACAACAGATG AGCTAGAGAA ATTGCTCTAT AAACCACAAG CTGGTGAATG GGGTCAGAAA 
TCAAGAGATG AAGAATGTGA TAGAATTATC AGTGGTATAG ATCAACTTTT GAATCTTGAT 
ATAGCAGCAG CTTTTGCAGG CCCTGTTGAT TTGTGTACAT ACCCGAAGTA CTGTACTGTA 
GTAGCTTATC CAACCGATCT TTACACAATT CGAATGAGAC TTGTTAATCG ATTTTACAGG 
AGGCTGTCTG CGTTAGTTTG GGAAGTCAGA TATATAGAAC ATAATGCCAG AACATTTAAC 
GAACCTGAGA GTGTAATTGC AAGATCAGCT AAAAAGATAA CTGACCAACT TTTAAAATTT 
ATCAAGAATC AACACTGTAC AAATATCTCA GAACTTTCTA ACACATCTGA AAATGATGAG 
CAAAATGCTG AGGATTTGGA TGATAGTGAT CTTCCTAAAA CATCTTCTGG AAGGAGGAGA 
GTCCATGATG GGAAAAAAAG CATCAGAGCT ACGAACTATG TTGAAAGCAA CTGGAAGAAA 
CAGTGTAAGG AACTAGTGAA CTTAATTTTT CAGTGTGAAG ATTCTGAACC ATTTAGACAA 
CCTGTTGATT TGGTTGAATA TCCAGACTAC AGAGATATTA TAGATACCCC AATGGATTTT 
GGAACAGTAA GGGAAACTCT AGATGCGGGA AATTATGACA GCCCTTTGGA GTTTTGCAAA 
GACATCCGGC TGATATTTAG CAATGCAAAA GCGTATACAC CAAACAAAAG ATCAAAGATT 
TATAGTATGA CCTTGAGATT ATCTGCCTTA TTTGAAGAAA AAATGAAGAA AATCTCTTCT 
GATTTTAAAA TTGGTCAAAA ATTCAATGAA AAACTTCGAA GAAGCCAGAG GTTCAAGCAA 
CGGCAAAATT GTAAAGGTGA CAGTCAGCCT AACAAAAGTA TCAGAAACCT CAAGCCGAAG 
AGGTTAAAAT CTCAGACAAA AATAATTCCT GAGTTGGTAG GTTCTCCTAC CCAGTCTACC 
TCAAGTAGGA CAGCTTATCT TGGAACCCAC AAGACAAGTG CTGGTATCTC TTCAGGTGTT 
ACTTCTGGTG ACTCTTCAGA TTCAGCAGAA TCATCAGAAA GGAGGAAAAG AAATAGACCT 
ATAACAAATG GTTCTACATT ATCTGAAAGT GAAGTGGAAG ATTCTTTAGC TACCTCTTTG 
TCATCGTCAG CTTCCAGTAG TTCTGAGGAA AGCAAAGAGA GTTCCAGAGC TCGTGAATCC 
TCCTCACGCA GTGGGCTATC CAGAAGCAGC AATCTCAGGG TAACCAGAAC TAGAGCTGCT 
CAAAGAAAAA CTGGTCCCGT TTCATTAGCA AATGGATGTG GCAGAAAAGC CACTCGAAAG 
AGAGTCTATT TAAGTGATTC TGATAACAAT TCATTGGAGA CTGGTGAAAT TCTAAAAGCC 
AGAGCTGGAA ATAACCGAAA AGTCTTAAGG AAGTGTGCTG CTGTGGCTGC CAATAAAATA 
AAGCTAATGA GTGATGTAGA AGAGAATTCT AGCTCTGAAA GTGTCTGTTC TGGTCGGAAG 
CTGCCTCACC GCAATGCTTC TGCTGTAGCT AGAAAAAAGT TATTACATAA TTCTGAAGAT 
GAACAGAGCT TAAAGTCAGA AATTGAAGAA GAGGAGCTAA AAGATGAAAA TCAACTATTA 
CCAGTGTCCA GTTCTCACAC TGCCCAGAGC AATGTTGATG AATCTGAAAA CAGAGACTCA 
GAGTCAGAAA GTGATTTGCG GGTAGCCCGG AAAAATTGGC ATGCTAATGG TTACAAGTCC 
CATACTCCAG CACCTTCAAA GACAAAATTT CTTAAAATAG AGTCTTCTGA GGAAGACTCT 
AAAAGTCATG ATTCAGATCA TGCATGTAAC AGAACTGCTG GCCCATCAAC GTCTGTGCAG 
AAACTTAAGG CAGAGAGCAT CTCAGAGGAA GCAGATTCTG AACCAGGAAG ATCTGGTGGT 
AGGAAATACA ATACATTTCA CAAGAATGCG AGTTTCTTTA AAAAAACCAA GATTCTGAGT 
GACTCAGAAG ACTCTGAATC TGAAGAGCAA GATAGAGAAG ATGGGAAATG TCATAAAATG 
GAAATGAACC CAATTTCAGG AAATCTGAAC TGTGACCCTA TTGCTATGTC CCAGTGTTCC 
TCAGATCATG GATGTGAAAC TGATTTAGAT TCAGATGATG ACAAAATAGA AAAACCAAAC 
AATTTTATGA AAGATTCTGC ATCACAAGAC AATGGACTAA GCAGAAAAAT TTCCAGGAAA 
AGGGTCTGTT CCAGTGACTC AGACAGTAGT TTACAGGTGG TTAAGAAATC ATCAAAAGCC 
AGAACAGGTC TCCTGAGGAT TACTCGAAGA TGTGCAGCTA CGGCTGCCAA TAAGATCAAG 
CTCATGAGTG ATGTAGAAGA TGTCAGTTTA GAAAATGTGC ACACTAGAAG CAAAAATGGA 
AGGAAAAAAC CTCTCCATCT TGCTTGTACT ACAGCTAAGA AGAAATTGAG TGATTGTGAA 
GGAAGTGTAC ATTGTGAAGT ACCAAGTGAA CAGTATGCCT GTGAAGGCAA GCCACCTGAT 
CCTGACTCCG AAGGTAGTAC AAAAGTGCTT AGTCAGGCTC TAAATGGAGA CTCAGACTCT 
GAAGATATGT TGAATTCAGA ACACAAGCAC AGGCATACCA ATATTCACAA AATAGATGCA 
CCTTCTAAAA GAAAAAGTTC CTCTGTTACA TCTTCAGGAG AAGATTCAAA AAGTCATATT 
CCAGGGAGTG AGACTGATAG GACATTTTCT TCAGAGTCAA CCTTGGCACA AAAAGCTACT 
GCAGAGAATA ATTTTGAAGT GGAACTGAAT TATGGGCTGC GCAGGTGGAA TGGCAGAAGA 
CTCAGGACCT ATGGAAAGGC TCCTTTTAGT AAGACAAAAG TGATTCATGA TTCACAGGAA 
ACAGCAGAGA AGGAAGTAAA AAGGAAGAGA TCGCATCCTG AATTGGAAAA TGTGAAAATC 
TCTGAAACAA CTGGGAATTC AAAGTTTAGA CCTGATACTA GTTCCAAATC ATCAGATTTG 
GGATCTGTAA CTGAATCAGA TATTGACTGT ACTGATAATA CAAAAACCAA AAGGAGGAAA 
ACGAAAGGAA AAGCAAAAGT AGTTAGAAAA GAATTTGTTC CTAGAGACAG AGAACCCAAT 
ACAAAAGTGA GAACATGTAT GCATAATCAG AAGGATGCAG TGCAGATGCC TAGTGAAACT 
CTGAAAGCAA AAATGGTACC TGAGAAAGTT CCCCGCAGAT GTGCTACTGT TGCTGCAAAT 
AAAATAAAGA TAATGAGTAA TCTAAAAGAA ACGATCTCAG GACCAGAAAA TGTCTGGATT 
AGGAAGAGTA GCAGAAAACT GCCCCATCGA AATGCTTCTG CTGCGGCTAA GAAAAAATTA 
CTGAATGTTT ATAAAGAGGA TGATACAACC ATAAATTCTG AAAGTGAAAA AGAGCTAGAA 
GATATTAATA GAAAAATGCT TTTTTTAAGG GGGTTCAGAT CCTGGAAAGA AAATGCACAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061836.2
CDS	296..7258
Translation	MAEPSSARRPVPLIESELYFLIARYLSAGPCRRAAQVLVQELEQYQLLPKRLDWEGNEHNRSYEELVLSNKHVAPDHLLQICQRIGPMLDKEIPPSISRVTSLLGAGRQSLLRTAKDCRHTVWKGSAFAALHRGRPPEMPVNYGSPPNLVEIHRGKQLTGCSTFSTAFPGTMYQHIKMHRRILGHLSAVYCVAFDRTGHRIFTGSDDCLVKIWSTHNGRLLSTLRGHSAEISDMAVNYENTMIAAGSCDKIIRVWCLRTCAPVAVLQGHTGSITSLQFSPMAKGSQRYMVSTGADGTVCFWQWDLESLKFSPRPLKFTEKPRPGVQMLCSSFSVGGMFLATGSTDHVIRMYFLGFEAPEKIAELESHTDKVDSIQFCNNGDRFLSGSRDGTARIWRFEQLEWRSILLDMATRISGDLSSEEERFMKPKVTMIAWNQNDSIVVTAVNDHVLKVWNSYTGQLLHNLMGHADEVFVLETHPFDSRIMLSAGHDGSIFIWDITKGTKMKHYFNMIEGQGHGAVFDCKFSQDGQHFACTDSHGHLLIFGFGCSKPYEKIPDQMFFHTDYRPLIRDSNNYVLDEQTQQAPHLMPPPFLVDVDGNPHPTKYQRLVPGRENSADEHLIPQLGYVATSDGEVIEQIISLQTNDNDERSPESSILDGMIRQLQQQQDQRMGADQDTIPRGLSNGEETPRRGFRRLSLDIQSPPNIGLRRSGQVEGVRQMHQNAPRSQIATERDLQAWKRRVVVPEVPLGIFRKLEDFRLEKGEEERNLYIIGRKRKTLQLSHKSDSVVLVSQSRQRTCRRKYPNYGRRNRSWRELSSGNESSSSVRHETSCDQSEGSGSSEEDEWRSDRKSESYSESSSDSSSRYSDWTADAGINLQPPLRTSCRRRITRFCSSSEDEISTENLSPPKRRRKRKKENKPKKENLRRMTPAELANMEHLYEFHPPVWITDTTLRKSPFVPQMGDEVIYFRQGHEAYIEAVRRNNIYELNPNKEPWRKMDLRDQELVKIVGIRYEVGPPTLCCLKLAFIDPATGKLMDKSFSIRYHDMPDVIDFLVLRQFYDEARQRNWQSCDRFRSIIDDAWWFGTVLSQEPYQPQYPDSHFQCYIVRWDNTEIEKLSPWDMEPIPDNVDPPEELGASISVTTDELEKLLYKPQAGEWGQKSRDEECDRIISGIDQLLNLDIAAAFAGPVDLCTYPKYCTVVAYPTDLYTIRMRLVNRFYRRLSALVWEVRYIEHNARTFNEPESVIARSAKKITDQLLKFIKNQHCTNISELSNTSENDEQNAEDLDDSDLPKTSSGRRRVHDGKKSIRATNYVESNWKKQCKELVNLIFQCEDSEPFRQPVDLVEYPDYRDIIDTPMDFGTVRETLDAGNYDSPLEFCKDIRLIFSNAKAYTPNKRSKIYSMTLRLSALFEEKMKKISSDFKIGQKFNEKLRRSQRFKQRQNCKGDSQPNKSIRNLKPKRLKSQTKIIPELVGSPTQSTSSRTAYLGTHKTSAGISSGVTSGDSSDSAESSERRKRNRPITNGSTLSESEVEDSLATSLSSSASSSSEESKESSRARESSSRSGLSRSSNLRVTRTRAAQRKTGPVSLANGCGRKATRKRVYLSDSDNNSLETGEILKARAGNNRKVLRKCAAVAANKIKLMSDVEENSSSESVCSGRKLPHRNASAVARKKLLHNSEDEQSLKSEIEEEELKDENQLLPVSSSHTAQSNVDESENRDSESESDLRVARKNWHANGYKSHTPAPSKTKFLKIESSEEDSKSHDSDHACNRTAGPSTSVQKLKAESISEEADSEPGRSGGRKYNTFHKNASFFKKTKILSDSEDSESEEQDREDGKCHKMEMNPISGNLNCDPIAMSQCSSDHGCETDLDSDDDKIEKPNNFMKDSASQDNGLSRKISRKRVCSSDSDSSLQVVKKSSKARTGLLRITRRCAATAANKIKLMSDVEDVSLENVHTRSKNGRKKPLHLACTTAKKKLSDCEGSVHCEVPSEQYACEGKPPDPDSEGSTKVLSQALNGDSDSEDMLNSEHKHRHTNIHKIDAPSKRKSSSVTSSGEDSKSHIPGSETDRTFSSESTLAQKATAENNFEVELNYGLRRWNGRRLRTYGKAPFSKTKVIHDSQETAEKEVKRKRSHPELENVKISETTGNSKFRPDTSSKSSDLGSVTESDIDCTDNTKTKRRKTKGKAKVVRKEFVPRDREPNTKVRTCMHNQKDAVQMPSETLKAKMVPEKVPRRCATVAANKIKIMSNLKETISGPENVWIRKSSRKLPHRNASAAAKKKLLNVYKEDDTTINSESEKELEDINRKMLFLRGFRSWKENAQ

Target ORF information:

RefSeq Version	NM_018963.4
Organism	Homo sapiens(human)
Definition	Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018963.4

ORF Insert Sequence:

ATGGCGGAGC CGTCGTCCGC CCGACGCCCG GTGCCTCTCA TCGAGTCGGA GCTGTACTTC 
CTTATCGCCC GGTACCTATC GGCGGGCCCG TGTCGGAGAG CGGCCCAGGT GCTGGTGCAG 
GAGCTGGAGC AGTACCAGTT GTTGCCGAAG AGATTGGACT GGGAGGGCAA CGAGCACAAC 
AGGAGCTACG AGGAGTTGGT CTTGTCCAAT AAGCATGTGG CTCCTGATCA TCTTTTGCAA 
ATCTGCCAGC GCATCGGTCC TATGTTGGAT AAAGAAATTC CACCCAGTAT TTCAAGAGTC 
ACTTCTTTAC TTGGTGCAGG AAGGCAGTCT TTGCTACGTA CAGCAAAAGA CTGCAGGCAC 
ACAGTTTGGA AGGGCTCTGC CTTTGCTGCT CTTCATAGAG GAAGACCTCC TGAAATGCCA 
GTGAATTATG GTTCCCCACC AAATCTTGTG GAGATACATC GAGGAAAACA ACTCACAGGG 
TGTTCCACTT TTAGTACAGC ATTTCCAGGA ACTATGTATC AGCATATAAA AATGCACAGA 
AGGATTCTCG GACATCTATC TGCTGTTTAC TGTGTAGCAT TTGATAGGAC AGGACATAGA 
ATCTTTACAG GTTCAGATGA CTGTTTGGTA AAGATTTGGT CAACACATAA TGGCCGCTTG 
TTATCTACAT TAAGAGGTCA TTCTGCAGAA ATTTCAGATA TGGCAGTAAA CTATGAGAAT 
ACAATGATTG CTGCGGGGAG CTGTGATAAA ATTATTAGAG TGTGGTGCTT GAGAACTTGT 
GCCCCAGTTG CTGTGCTCCA AGGACACACA GGATCAATTA CATCTTTACA GTTTAGCCCG 
ATGGCCAAAG GCTCTCAAAG ATACATGGTT TCCACTGGTG CTGATGGGAC AGTTTGCTTT 
TGGCAATGGG ATTTAGAATC CTTAAAATTT AGCCCACGTC CCCTGAAGTT CACTGAAAAG 
CCTAGGCCAG GCGTTCAAAT GCTTTGTTCT TCTTTTAGTG TTGGTGGTAT GTTTTTAGCC 
ACAGGTAGTA CTGATCATGT AATCAGAATG TATTTTTTGG GTTTTGAAGC ACCCGAAAAA 
ATCGCAGAAC TTGAAAGCCA CACTGATAAA GTAGATAGTA TCCAATTTTG TAACAATGGT 
GATCGGTTCC TAAGTGGTAG CAGAGATGGA ACAGCACGGA TTTGGAGATT TGAGCAGTTA 
GAATGGAGGA GCATTTTATT GGATATGGCT ACCAGAATCT CAGGGGACTT ATCTTCCGAA 
GAGGAAAGGT TTATGAAACC TAAAGTAACA ATGATAGCTT GGAATCAAAA TGATAGCATT 
GTTGTCACAG CTGTGAATGA TCATGTCCTC AAAGTGTGGA ATTCTTACAC TGGACAACTG 
CTTCATAACT TAATGGGACA TGCTGATGAA GTATTTGTTC TGGAGACACA TCCCTTTGAT 
TCCAGAATTA TGTTATCTGC AGGACATGAT GGCAGCATAT TTATATGGGA TATTACAAAA 
GGTACCAAGA TGAAACATTA TTTTAATATG ATTGAAGGAC AAGGACATGG AGCTGTGTTT 
GACTGTAAGT TTTCACAGGA TGGACAGCAT TTTGCCTGTA CAGATTCTCA TGGGCACCTT 
CTGATATTTG GTTTTGGATG CAGCAAACCA TATGAAAAGA TTCCTGATCA GATGTTCTTC 
CATACTGACT ATCGACCACT TATTAGAGAT TCTAATAATT ATGTCTTAGA TGAGCAAACT 
CAGCAGGCTC CTCATCTTAT GCCTCCACCA TTCTTGGTAG ATGTAGATGG AAATCCTCAT 
CCAACCAAGT ATCAGAGATT AGTACCAGGC CGAGAAAATT CTGCAGATGA ACATTTGATT 
CCACAGCTGG GCTATGTGGC AACAAGTGAT GGAGAGGTGA TTGAACAAAT TATAAGCCTG 
CAAACCAATG ATAATGATGA ACGCAGCCCA GAATCGAGTA TTCTTGATGG AATGATAAGA 
CAGTTGCAGC AGCAGCAAGA TCAGAGAATG GGAGCAGATC AGGATACTAT TCCAAGAGGA 
CTTTCAAATG GTGAAGAAAC ACCCCGGAGA GGTTTTAGAA GGCTGAGCTT AGACATTCAG 
TCCCCTCCAA ATATTGGTCT GCGTCGTAGT GGACAAGTTG AAGGTGTTCG TCAGATGCAT 
CAAAACGCTC CACGCAGTCA GATTGCTACA GAACGTGACC TGCAGGCTTG GAAACGAAGA 
GTGGTTGTAC CAGAGGTACC ACTAGGCATA TTTAGGAAGC TGGAAGACTT CCGATTAGAG 
AAAGGTGAAG AGGAAAGAAA TCTTTATATA ATAGGAAGAA AAAGAAAGAC TCTTCAGCTC 
TCACATAAGT CGGATTCAGT GGTTTTGGTA TCACAGTCTA GACAAAGGAC ATGTAGGCGT 
AAATATCCAA ATTATGGTAG AAGAAATCGT AGCTGGCGTG AGTTATCTTC TGGAAATGAG 
TCTTCAAGCT CTGTAAGACA TGAGACTTCC TGTGATCAGA GTGAAGGTTC TGGTTCTTCA 
GAAGAGGATG AATGGAGAAG TGACAGAAAA AGTGAGAGTT ACAGCGAAAG TTCAAGTGAC 
TCTTCATCTA GATATTCCGA TTGGACAGCT GATGCGGGCA TCAATTTGCA GCCTCCTTTA 
AGAACATCAT GTCGTCGACG AATTACTCGA TTTTGTAGTA GTTCAGAAGA TGAAATATCT 
ACTGAGAATT TATCTCCTCC AAAAAGAAGA CGAAAGAGAA AGAAAGAAAA TAAGCCTAAG 
AAGGAGAATT TGCGGAGGAT GACTCCAGCA GAGCTTGCAA ATATGGAGCA TTTATATGAA 
TTTCACCCTC CAGTTTGGAT TACTGACACC ACACTTAGAA AATCTCCTTT TGTTCCTCAA 
ATGGGTGATG AGGTAATATA TTTTCGACAG GGTCATGAAG CTTATATTGA GGCTGTAAGA 
AGAAATAATA TTTATGAACT GAACCCTAAT AAGGAGCCAT GGAGAAAAAT GGATCTTAGG 
GATCAAGAAT TGGTTAAAAT AGTTGGAATA CGATATGAAG TTGGGCCCCC TACACTCTGT 
TGCCTAAAAC TAGCATTTAT AGATCCAGCA ACTGGAAAAC TTATGGACAA ATCTTTCTCT 
ATTAGATATC ATGATATGCC AGATGTTATT GACTTTCTTG TATTGCGTCA ATTTTATGAT 
GAAGCAAGAC AGAGGAATTG GCAGTCTTGT GACAGATTCC GCTCTATTAT TGATGATGCT 
TGGTGGTTTG GAACAGTGTT AAGTCAAGAG CCATATCAAC CACAGTATCC TGATAGTCAT 
TTCCAGTGTT ATATTGTTAG GTGGGATAAT ACTGAAATTG AAAAACTTAG CCCATGGGAC 
ATGGAACCAA TTCCTGATAA TGTTGATCCA CCTGAAGAAT TAGGAGCTAG TATTTCTGTC 
ACAACAGATG AGCTAGAGAA ATTGCTCTAT AAACCACAAG CTGGTGAATG GGGTCAGAAA 
TCAAGAGATG AAGAATGTGA TAGAATTATC AGTGGTATAG ATCAACTTTT GAATCTTGAT 
ATAGCAGCAG CTTTTGCAGG CCCTGTTGAT TTGTGTACAT ACCCGAAGTA CTGTACTGTA 
GTAGCTTATC CAACCGATCT TTACACAATT CGAATGAGAC TTGTTAATCG ATTTTACAGG 
AGGCTGTCTG CGTTAGTTTG GGAAGTCAGA TATATAGAAC ATAATGCCAG AACATTTAAC 
GAACCTGAGA GTGTAATTGC AAGATCAGCT AAAAAGATAA CTGACCAACT TTTAAAATTT 
ATCAAGAATC AACACTGTAC AAATATCTCA GAACTTTCTA ACACATCTGA AAATGATGAG 
CAAAATGCTG AGGATTTGGA TGATAGTGAT CTTCCTAAAA CATCTTCTGG AAGGAGGAGA 
GTCCATGATG GGAAAAAAAG CATCAGAGCT ACGAACTATG TTGAAAGCAA CTGGAAGAAA 
CAGTGTAAGG AACTAGTGAA CTTAATTTTT CAGTGTGAAG ATTCTGAACC ATTTAGACAA 
CCTGTTGATT TGGTTGAATA TCCAGACTAC AGAGATATTA TAGATACCCC AATGGATTTT 
GGAACAGTAA GGGAAACTCT AGATGCGGGA AATTATGACA GCCCTTTGGA GTTTTGCAAA 
GACATCCGGC TGATATTTAG CAATGCAAAA GCGTATACAC CAAACAAAAG ATCAAAGATT 
TATAGTATGA CCTTGAGATT ATCTGCCTTA TTTGAAGAAA AAATGAAGAA AATCTCTTCT 
GATTTTAAAA TTGGTCAAAA ATTCAATGAA AAACTTCGAA GAAGCCAGAG GTTCAAGCAA 
CGGCAAAATT GTAAAGGTGA CAGTCAGCCT AACAAAAGTA TCAGAAACCT CAAGCCGAAG 
AGGTTAAAAT CTCAGACAAA AATAATTCCT GAGTTGGTAG GTTCTCCTAC CCAGTCTACC 
TCAAGTAGGA CAGCTTATCT TGGAACCCAC AAGACAAGTG CTGGTATCTC TTCAGGTGTT 
ACTTCTGGTG ACTCTTCAGA TTCAGCAGAA TCATCAGAAA GGAGGAAAAG AAATAGACCT 
ATAACAAATG GTTCTACATT ATCTGAAAGT GAAGTGGAAG ATTCTTTAGC TACCTCTTTG 
TCATCGTCAG CTTCCAGTAG TTCTGAGGAA AGCAAAGAGA GTTCCAGAGC TCGTGAATCC 
TCCTCACGCA GTGGGCTATC CAGAAGCAGC AATCTCAGGG TAACCAGAAC TAGAGCTGCT 
CAAAGAAAAA CTGGTCCCGT TTCATTAGCA AATGGATGTG GCAGAAAAGC CACTCGAAAG 
AGAGTCTATT TAAGTGATTC TGATAACAAT TCATTGGAGA CTGGTGAAAT TCTAAAAGCC 
AGAGCTGGAA ATAACCGAAA AGTCTTAAGG AAGTGTGCTG CTGTGGCTGC CAATAAAATA 
AAGCTAATGA GTGATGTAGA AGAGAATTCT AGCTCTGAAA GTGTCTGTTC TGGTCGGAAG 
CTGCCTCACC GCAATGCTTC TGCTGTAGCT AGAAAAAAGT TATTACATAA TTCTGAAGAT 
GAACAGAGCT TAAAGTCAGA AATTGAAGAA GAGGAGCTAA AAGATGAAAA TCAACTATTA 
CCAGTGTCCA GTTCTCACAC TGCCCAGAGC AATGTTGATG AATCTGAAAA CAGAGACTCA 
GAGTCAGAAA GTGATTTGCG GGTAGCCCGG AAAAATTGGC ATGCTAATGG TTACAAGTCC 
CATACTCCAG CACCTTCAAA GACAAAATTT CTTAAAATAG AGTCTTCTGA GGAAGACTCT 
AAAAGTCATG ATTCAGATCA TGCATGTAAC AGAACTGCTG GCCCATCAAC GTCTGTGCAG 
AAACTTAAGG CAGAGAGCAT CTCAGAGGAA GCAGATTCTG AACCAGGAAG ATCTGGTGGT 
AGGAAATACA ATACATTTCA CAAGAATGCG AGTTTCTTTA AAAAAACCAA GATTCTGAGT 
GACTCAGAAG ACTCTGAATC TGAAGAGCAA GATAGAGAAG ATGGGAAATG TCATAAAATG 
GAAATGAACC CAATTTCAGG AAATCTGAAC TGTGACCCTA TTGCTATGTC CCAGTGTTCC 
TCAGATCATG GATGTGAAAC TGATTTAGAT TCAGATGATG ACAAAATAGA AAAACCAAAC 
AATTTTATGA AAGATTCTGC ATCACAAGAC AATGGACTAA GCAGAAAAAT TTCCAGGAAA 
AGGGTCTGTT CCAGTGACTC AGACAGTAGT TTACAGGTGG TTAAGAAATC ATCAAAAGCC 
AGAACAGGTC TCCTGAGGAT TACTCGAAGA TGTGCAGCTA CGGCTGCCAA TAAGATCAAG 
CTCATGAGTG ATGTAGAAGA TGTCAGTTTA GAAAATGTGC ACACTAGAAG CAAAAATGGA 
AGGAAAAAAC CTCTCCATCT TGCTTGTACT ACAGCTAAGA AGAAATTGAG TGATTGTGAA 
GGAAGTGTAC ATTGTGAAGT ACCAAGTGAA CAGTATGCCT GTGAAGGCAA GCCACCTGAT 
CCTGACTCCG AAGGTAGTAC AAAAGTGCTT AGTCAGGCTC TAAATGGAGA CTCAGACTCT 
GAAGATATGT TGAATTCAGA ACACAAGCAC AGGCATACCA ATATTCACAA AATAGATGCA 
CCTTCTAAAA GAAAAAGTTC CTCTGTTACA TCTTCAGGAG AAGATTCAAA AAGTCATATT 
CCAGGGAGTG AGACTGATAG GACATTTTCT TCAGAGTCAA CCTTGGCACA AAAAGCTACT 
GCAGAGAATA ATTTTGAAGT GGAACTGAAT TATGGGCTGC GCAGGTGGAA TGGCAGAAGA 
CTCAGGACCT ATGGAAAGGC TCCTTTTAGT AAGACAAAAG TGATTCATGA TTCACAGGAA 
ACAGCAGAGA AGGAAGTAAA AAGGAAGAGA TCGCATCCTG AATTGGAAAA TGTGAAAATC 
TCTGAAACAA CTGGGAATTC AAAGTTTAGA CCTGATACTA GTTCCAAATC ATCAGATTTG 
GGATCTGTAA CTGAATCAGA TATTGACTGT ACTGATAATA CAAAAACCAA AAGGAGGAAA 
ACGAAAGGAA AAGCAAAAGT AGTTAGAAAA GAATTTGTTC CTAGAGACAG AGAACCCAAT 
ACAAAAGTGA GAACATGTAT GCATAATCAG AAGGATGCAG TGCAGATGCC TAGTGAAACT 
CTGAAAGCAA AAATGGTACC TGAGAAAGTT CCCCGCAGAT GTGCTACTGT TGCTGCAAAT 
AAAATAAAGA TAATGAGTAA TCTAAAAGAA ACGATCTCAG GACCAGAAAA TGTCTGGATT 
AGGAAGAGTA GCAGAAAACT GCCCCATCGA AATGCTTCTG CTGCGGCTAA GAAAAAATTA 
CTGAATGTTT ATAAAGAGGA TGATACAACC ATAAATTCTG AAAGTGAAAA AGAGCTAGAA 
GATATTAATA GAAAAATGCT TTTTTTAAGG GGGTTCAGAT CCTGGAAAGA AAATGCACAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRWD1 ( NM_018963.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_061836.2 Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant 1, mRNA.

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CloneID	OHu09386
Clone ID Related Accession (Same CDS sequence)	NM_018963.4 , NM_018963.5
Accession Version	NM_018963.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	bromodomain and WD repeat-containing protein 1 isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA792365.1, AJ292465.1, AK125906.1, AJ238214.2, AU139205.1, BQ930702.1, BP397411.1, AA807344.1 and AF129408.4. On Jun 2, 2019 this sequence version replaced NM_018963.4. Transcript Variant: This variant (1) encodes the longest isoform (A). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ292465.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGC CGTCGTCCGC CCGACGCCCG GTGCCTCTCA TCGAGTCGGA GCTGTACTTC 
CTTATCGCCC GGTACCTATC GGCGGGCCCG TGTCGGAGAG CGGCCCAGGT GCTGGTGCAG 
GAGCTGGAGC AGTACCAGTT GTTGCCGAAG AGATTGGACT GGGAGGGCAA CGAGCACAAC 
AGGAGCTACG AGGAGTTGGT CTTGTCCAAT AAGCATGTGG CTCCTGATCA TCTTTTGCAA 
ATCTGCCAGC GCATCGGTCC TATGTTGGAT AAAGAAATTC CACCCAGTAT TTCAAGAGTC 
ACTTCTTTAC TTGGTGCAGG AAGGCAGTCT TTGCTACGTA CAGCAAAAGA CTGCAGGCAC 
ACAGTTTGGA AGGGCTCTGC CTTTGCTGCT CTTCATAGAG GAAGACCTCC TGAAATGCCA 
GTGAATTATG GTTCCCCACC AAATCTTGTG GAGATACATC GAGGAAAACA ACTCACAGGG 
TGTTCCACTT TTAGTACAGC ATTTCCAGGA ACTATGTATC AGCATATAAA AATGCACAGA 
AGGATTCTCG GACATCTATC TGCTGTTTAC TGTGTAGCAT TTGATAGGAC AGGACATAGA 
ATCTTTACAG GTTCAGATGA CTGTTTGGTA AAGATTTGGT CAACACATAA TGGCCGCTTG 
TTATCTACAT TAAGAGGTCA TTCTGCAGAA ATTTCAGATA TGGCAGTAAA CTATGAGAAT 
ACAATGATTG CTGCGGGGAG CTGTGATAAA ATTATTAGAG TGTGGTGCTT GAGAACTTGT 
GCCCCAGTTG CTGTGCTCCA AGGACACACA GGATCAATTA CATCTTTACA GTTTAGCCCG 
ATGGCCAAAG GCTCTCAAAG ATACATGGTT TCCACTGGTG CTGATGGGAC AGTTTGCTTT 
TGGCAATGGG ATTTAGAATC CTTAAAATTT AGCCCACGTC CCCTGAAGTT CACTGAAAAG 
CCTAGGCCAG GCGTTCAAAT GCTTTGTTCT TCTTTTAGTG TTGGTGGTAT GTTTTTAGCC 
ACAGGTAGTA CTGATCATGT AATCAGAATG TATTTTTTGG GTTTTGAAGC ACCCGAAAAA 
ATCGCAGAAC TTGAAAGCCA CACTGATAAA GTAGATAGTA TCCAATTTTG TAACAATGGT 
GATCGGTTCC TAAGTGGTAG CAGAGATGGA ACAGCACGGA TTTGGAGATT TGAGCAGTTA 
GAATGGAGGA GCATTTTATT GGATATGGCT ACCAGAATCT CAGGGGACTT ATCTTCCGAA 
GAGGAAAGGT TTATGAAACC TAAAGTAACA ATGATAGCTT GGAATCAAAA TGATAGCATT 
GTTGTCACAG CTGTGAATGA TCATGTCCTC AAAGTGTGGA ATTCTTACAC TGGACAACTG 
CTTCATAACT TAATGGGACA TGCTGATGAA GTATTTGTTC TGGAGACACA TCCCTTTGAT 
TCCAGAATTA TGTTATCTGC AGGACATGAT GGCAGCATAT TTATATGGGA TATTACAAAA 
GGTACCAAGA TGAAACATTA TTTTAATATG ATTGAAGGAC AAGGACATGG AGCTGTGTTT 
GACTGTAAGT TTTCACAGGA TGGACAGCAT TTTGCCTGTA CAGATTCTCA TGGGCACCTT 
CTGATATTTG GTTTTGGATG CAGCAAACCA TATGAAAAGA TTCCTGATCA GATGTTCTTC 
CATACTGACT ATCGACCACT TATTAGAGAT TCTAATAATT ATGTCTTAGA TGAGCAAACT 
CAGCAGGCTC CTCATCTTAT GCCTCCACCA TTCTTGGTAG ATGTAGATGG AAATCCTCAT 
CCAACCAAGT ATCAGAGATT AGTACCAGGC CGAGAAAATT CTGCAGATGA ACATTTGATT 
CCACAGCTGG GCTATGTGGC AACAAGTGAT GGAGAGGTGA TTGAACAAAT TATAAGCCTG 
CAAACCAATG ATAATGATGA ACGCAGCCCA GAATCGAGTA TTCTTGATGG AATGATAAGA 
CAGTTGCAGC AGCAGCAAGA TCAGAGAATG GGAGCAGATC AGGATACTAT TCCAAGAGGA 
CTTTCAAATG GTGAAGAAAC ACCCCGGAGA GGTTTTAGAA GGCTGAGCTT AGACATTCAG 
TCCCCTCCAA ATATTGGTCT GCGTCGTAGT GGACAAGTTG AAGGTGTTCG TCAGATGCAT 
CAAAACGCTC CACGCAGTCA GATTGCTACA GAACGTGACC TGCAGGCTTG GAAACGAAGA 
GTGGTTGTAC CAGAGGTACC ACTAGGCATA TTTAGGAAGC TGGAAGACTT CCGATTAGAG 
AAAGGTGAAG AGGAAAGAAA TCTTTATATA ATAGGAAGAA AAAGAAAGAC TCTTCAGCTC 
TCACATAAGT CGGATTCAGT GGTTTTGGTA TCACAGTCTA GACAAAGGAC ATGTAGGCGT 
AAATATCCAA ATTATGGTAG AAGAAATCGT AGCTGGCGTG AGTTATCTTC TGGAAATGAG 
TCTTCAAGCT CTGTAAGACA TGAGACTTCC TGTGATCAGA GTGAAGGTTC TGGTTCTTCA 
GAAGAGGATG AATGGAGAAG TGACAGAAAA AGTGAGAGTT ACAGCGAAAG TTCAAGTGAC 
TCTTCATCTA GATATTCCGA TTGGACAGCT GATGCGGGCA TCAATTTGCA GCCTCCTTTA 
AGAACATCAT GTCGTCGACG AATTACTCGA TTTTGTAGTA GTTCAGAAGA TGAAATATCT 
ACTGAGAATT TATCTCCTCC AAAAAGAAGA CGAAAGAGAA AGAAAGAAAA TAAGCCTAAG 
AAGGAGAATT TGCGGAGGAT GACTCCAGCA GAGCTTGCAA ATATGGAGCA TTTATATGAA 
TTTCACCCTC CAGTTTGGAT TACTGACACC ACACTTAGAA AATCTCCTTT TGTTCCTCAA 
ATGGGTGATG AGGTAATATA TTTTCGACAG GGTCATGAAG CTTATATTGA GGCTGTAAGA 
AGAAATAATA TTTATGAACT GAACCCTAAT AAGGAGCCAT GGAGAAAAAT GGATCTTAGG 
GATCAAGAAT TGGTTAAAAT AGTTGGAATA CGATATGAAG TTGGGCCCCC TACACTCTGT 
TGCCTAAAAC TAGCATTTAT AGATCCAGCA ACTGGAAAAC TTATGGACAA ATCTTTCTCT 
ATTAGATATC ATGATATGCC AGATGTTATT GACTTTCTTG TATTGCGTCA ATTTTATGAT 
GAAGCAAGAC AGAGGAATTG GCAGTCTTGT GACAGATTCC GCTCTATTAT TGATGATGCT 
TGGTGGTTTG GAACAGTGTT AAGTCAAGAG CCATATCAAC CACAGTATCC TGATAGTCAT 
TTCCAGTGTT ATATTGTTAG GTGGGATAAT ACTGAAATTG AAAAACTTAG CCCATGGGAC 
ATGGAACCAA TTCCTGATAA TGTTGATCCA CCTGAAGAAT TAGGAGCTAG TATTTCTGTC 
ACAACAGATG AGCTAGAGAA ATTGCTCTAT AAACCACAAG CTGGTGAATG GGGTCAGAAA 
TCAAGAGATG AAGAATGTGA TAGAATTATC AGTGGTATAG ATCAACTTTT GAATCTTGAT 
ATAGCAGCAG CTTTTGCAGG CCCTGTTGAT TTGTGTACAT ACCCGAAGTA CTGTACTGTA 
GTAGCTTATC CAACCGATCT TTACACAATT CGAATGAGAC TTGTTAATCG ATTTTACAGG 
AGGCTGTCTG CGTTAGTTTG GGAAGTCAGA TATATAGAAC ATAATGCCAG AACATTTAAC 
GAACCTGAGA GTGTAATTGC AAGATCAGCT AAAAAGATAA CTGACCAACT TTTAAAATTT 
ATCAAGAATC AACACTGTAC AAATATCTCA GAACTTTCTA ACACATCTGA AAATGATGAG 
CAAAATGCTG AGGATTTGGA TGATAGTGAT CTTCCTAAAA CATCTTCTGG AAGGAGGAGA 
GTCCATGATG GGAAAAAAAG CATCAGAGCT ACGAACTATG TTGAAAGCAA CTGGAAGAAA 
CAGTGTAAGG AACTAGTGAA CTTAATTTTT CAGTGTGAAG ATTCTGAACC ATTTAGACAA 
CCTGTTGATT TGGTTGAATA TCCAGACTAC AGAGATATTA TAGATACCCC AATGGATTTT 
GGAACAGTAA GGGAAACTCT AGATGCGGGA AATTATGACA GCCCTTTGGA GTTTTGCAAA 
GACATCCGGC TGATATTTAG CAATGCAAAA GCGTATACAC CAAACAAAAG ATCAAAGATT 
TATAGTATGA CCTTGAGATT ATCTGCCTTA TTTGAAGAAA AAATGAAGAA AATCTCTTCT 
GATTTTAAAA TTGGTCAAAA ATTCAATGAA AAACTTCGAA GAAGCCAGAG GTTCAAGCAA 
CGGCAAAATT GTAAAGGTGA CAGTCAGCCT AACAAAAGTA TCAGAAACCT CAAGCCGAAG 
AGGTTAAAAT CTCAGACAAA AATAATTCCT GAGTTGGTAG GTTCTCCTAC CCAGTCTACC 
TCAAGTAGGA CAGCTTATCT TGGAACCCAC AAGACAAGTG CTGGTATCTC TTCAGGTGTT 
ACTTCTGGTG ACTCTTCAGA TTCAGCAGAA TCATCAGAAA GGAGGAAAAG AAATAGACCT 
ATAACAAATG GTTCTACATT ATCTGAAAGT GAAGTGGAAG ATTCTTTAGC TACCTCTTTG 
TCATCGTCAG CTTCCAGTAG TTCTGAGGAA AGCAAAGAGA GTTCCAGAGC TCGTGAATCC 
TCCTCACGCA GTGGGCTATC CAGAAGCAGC AATCTCAGGG TAACCAGAAC TAGAGCTGCT 
CAAAGAAAAA CTGGTCCCGT TTCATTAGCA AATGGATGTG GCAGAAAAGC CACTCGAAAG 
AGAGTCTATT TAAGTGATTC TGATAACAAT TCATTGGAGA CTGGTGAAAT TCTAAAAGCC 
AGAGCTGGAA ATAACCGAAA AGTCTTAAGG AAGTGTGCTG CTGTGGCTGC CAATAAAATA 
AAGCTAATGA GTGATGTAGA AGAGAATTCT AGCTCTGAAA GTGTCTGTTC TGGTCGGAAG 
CTGCCTCACC GCAATGCTTC TGCTGTAGCT AGAAAAAAGT TATTACATAA TTCTGAAGAT 
GAACAGAGCT TAAAGTCAGA AATTGAAGAA GAGGAGCTAA AAGATGAAAA TCAACTATTA 
CCAGTGTCCA GTTCTCACAC TGCCCAGAGC AATGTTGATG AATCTGAAAA CAGAGACTCA 
GAGTCAGAAA GTGATTTGCG GGTAGCCCGG AAAAATTGGC ATGCTAATGG TTACAAGTCC 
CATACTCCAG CACCTTCAAA GACAAAATTT CTTAAAATAG AGTCTTCTGA GGAAGACTCT 
AAAAGTCATG ATTCAGATCA TGCATGTAAC AGAACTGCTG GCCCATCAAC GTCTGTGCAG 
AAACTTAAGG CAGAGAGCAT CTCAGAGGAA GCAGATTCTG AACCAGGAAG ATCTGGTGGT 
AGGAAATACA ATACATTTCA CAAGAATGCG AGTTTCTTTA AAAAAACCAA GATTCTGAGT 
GACTCAGAAG ACTCTGAATC TGAAGAGCAA GATAGAGAAG ATGGGAAATG TCATAAAATG 
GAAATGAACC CAATTTCAGG AAATCTGAAC TGTGACCCTA TTGCTATGTC CCAGTGTTCC 
TCAGATCATG GATGTGAAAC TGATTTAGAT TCAGATGATG ACAAAATAGA AAAACCAAAC 
AATTTTATGA AAGATTCTGC ATCACAAGAC AATGGACTAA GCAGAAAAAT TTCCAGGAAA 
AGGGTCTGTT CCAGTGACTC AGACAGTAGT TTACAGGTGG TTAAGAAATC ATCAAAAGCC 
AGAACAGGTC TCCTGAGGAT TACTCGAAGA TGTGCAGCTA CGGCTGCCAA TAAGATCAAG 
CTCATGAGTG ATGTAGAAGA TGTCAGTTTA GAAAATGTGC ACACTAGAAG CAAAAATGGA 
AGGAAAAAAC CTCTCCATCT TGCTTGTACT ACAGCTAAGA AGAAATTGAG TGATTGTGAA 
GGAAGTGTAC ATTGTGAAGT ACCAAGTGAA CAGTATGCCT GTGAAGGCAA GCCACCTGAT 
CCTGACTCCG AAGGTAGTAC AAAAGTGCTT AGTCAGGCTC TAAATGGAGA CTCAGACTCT 
GAAGATATGT TGAATTCAGA ACACAAGCAC AGGCATACCA ATATTCACAA AATAGATGCA 
CCTTCTAAAA GAAAAAGTTC CTCTGTTACA TCTTCAGGAG AAGATTCAAA AAGTCATATT 
CCAGGGAGTG AGACTGATAG GACATTTTCT TCAGAGTCAA CCTTGGCACA AAAAGCTACT 
GCAGAGAATA ATTTTGAAGT GGAACTGAAT TATGGGCTGC GCAGGTGGAA TGGCAGAAGA 
CTCAGGACCT ATGGAAAGGC TCCTTTTAGT AAGACAAAAG TGATTCATGA TTCACAGGAA 
ACAGCAGAGA AGGAAGTAAA AAGGAAGAGA TCGCATCCTG AATTGGAAAA TGTGAAAATC 
TCTGAAACAA CTGGGAATTC AAAGTTTAGA CCTGATACTA GTTCCAAATC ATCAGATTTG 
GGATCTGTAA CTGAATCAGA TATTGACTGT ACTGATAATA CAAAAACCAA AAGGAGGAAA 
ACGAAAGGAA AAGCAAAAGT AGTTAGAAAA GAATTTGTTC CTAGAGACAG AGAACCCAAT 
ACAAAAGTGA GAACATGTAT GCATAATCAG AAGGATGCAG TGCAGATGCC TAGTGAAACT 
CTGAAAGCAA AAATGGTACC TGAGAAAGTT CCCCGCAGAT GTGCTACTGT TGCTGCAAAT 
AAAATAAAGA TAATGAGTAA TCTAAAAGAA ACGATCTCAG GACCAGAAAA TGTCTGGATT 
AGGAAGAGTA GCAGAAAACT GCCCCATCGA AATGCTTCTG CTGCGGCTAA GAAAAAATTA 
CTGAATGTTT ATAAAGAGGA TGATACAACC ATAAATTCTG AAAGTGAAAA AGAGCTAGAA 
GATATTAATA GAAAAATGCT TTTTTTAAGG GGGTTCAGAT CCTGGAAAGA AAATGCACAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061836.2
CDS	142..7104
Translation	MAEPSSARRPVPLIESELYFLIARYLSAGPCRRAAQVLVQELEQYQLLPKRLDWEGNEHNRSYEELVLSNKHVAPDHLLQICQRIGPMLDKEIPPSISRVTSLLGAGRQSLLRTAKDCRHTVWKGSAFAALHRGRPPEMPVNYGSPPNLVEIHRGKQLTGCSTFSTAFPGTMYQHIKMHRRILGHLSAVYCVAFDRTGHRIFTGSDDCLVKIWSTHNGRLLSTLRGHSAEISDMAVNYENTMIAAGSCDKIIRVWCLRTCAPVAVLQGHTGSITSLQFSPMAKGSQRYMVSTGADGTVCFWQWDLESLKFSPRPLKFTEKPRPGVQMLCSSFSVGGMFLATGSTDHVIRMYFLGFEAPEKIAELESHTDKVDSIQFCNNGDRFLSGSRDGTARIWRFEQLEWRSILLDMATRISGDLSSEEERFMKPKVTMIAWNQNDSIVVTAVNDHVLKVWNSYTGQLLHNLMGHADEVFVLETHPFDSRIMLSAGHDGSIFIWDITKGTKMKHYFNMIEGQGHGAVFDCKFSQDGQHFACTDSHGHLLIFGFGCSKPYEKIPDQMFFHTDYRPLIRDSNNYVLDEQTQQAPHLMPPPFLVDVDGNPHPTKYQRLVPGRENSADEHLIPQLGYVATSDGEVIEQIISLQTNDNDERSPESSILDGMIRQLQQQQDQRMGADQDTIPRGLSNGEETPRRGFRRLSLDIQSPPNIGLRRSGQVEGVRQMHQNAPRSQIATERDLQAWKRRVVVPEVPLGIFRKLEDFRLEKGEEERNLYIIGRKRKTLQLSHKSDSVVLVSQSRQRTCRRKYPNYGRRNRSWRELSSGNESSSSVRHETSCDQSEGSGSSEEDEWRSDRKSESYSESSSDSSSRYSDWTADAGINLQPPLRTSCRRRITRFCSSSEDEISTENLSPPKRRRKRKKENKPKKENLRRMTPAELANMEHLYEFHPPVWITDTTLRKSPFVPQMGDEVIYFRQGHEAYIEAVRRNNIYELNPNKEPWRKMDLRDQELVKIVGIRYEVGPPTLCCLKLAFIDPATGKLMDKSFSIRYHDMPDVIDFLVLRQFYDEARQRNWQSCDRFRSIIDDAWWFGTVLSQEPYQPQYPDSHFQCYIVRWDNTEIEKLSPWDMEPIPDNVDPPEELGASISVTTDELEKLLYKPQAGEWGQKSRDEECDRIISGIDQLLNLDIAAAFAGPVDLCTYPKYCTVVAYPTDLYTIRMRLVNRFYRRLSALVWEVRYIEHNARTFNEPESVIARSAKKITDQLLKFIKNQHCTNISELSNTSENDEQNAEDLDDSDLPKTSSGRRRVHDGKKSIRATNYVESNWKKQCKELVNLIFQCEDSEPFRQPVDLVEYPDYRDIIDTPMDFGTVRETLDAGNYDSPLEFCKDIRLIFSNAKAYTPNKRSKIYSMTLRLSALFEEKMKKISSDFKIGQKFNEKLRRSQRFKQRQNCKGDSQPNKSIRNLKPKRLKSQTKIIPELVGSPTQSTSSRTAYLGTHKTSAGISSGVTSGDSSDSAESSERRKRNRPITNGSTLSESEVEDSLATSLSSSASSSSEESKESSRARESSSRSGLSRSSNLRVTRTRAAQRKTGPVSLANGCGRKATRKRVYLSDSDNNSLETGEILKARAGNNRKVLRKCAAVAANKIKLMSDVEENSSSESVCSGRKLPHRNASAVARKKLLHNSEDEQSLKSEIEEEELKDENQLLPVSSSHTAQSNVDESENRDSESESDLRVARKNWHANGYKSHTPAPSKTKFLKIESSEEDSKSHDSDHACNRTAGPSTSVQKLKAESISEEADSEPGRSGGRKYNTFHKNASFFKKTKILSDSEDSESEEQDREDGKCHKMEMNPISGNLNCDPIAMSQCSSDHGCETDLDSDDDKIEKPNNFMKDSASQDNGLSRKISRKRVCSSDSDSSLQVVKKSSKARTGLLRITRRCAATAANKIKLMSDVEDVSLENVHTRSKNGRKKPLHLACTTAKKKLSDCEGSVHCEVPSEQYACEGKPPDPDSEGSTKVLSQALNGDSDSEDMLNSEHKHRHTNIHKIDAPSKRKSSSVTSSGEDSKSHIPGSETDRTFSSESTLAQKATAENNFEVELNYGLRRWNGRRLRTYGKAPFSKTKVIHDSQETAEKEVKRKRSHPELENVKISETTGNSKFRPDTSSKSSDLGSVTESDIDCTDNTKTKRRKTKGKAKVVRKEFVPRDREPNTKVRTCMHNQKDAVQMPSETLKAKMVPEKVPRRCATVAANKIKIMSNLKETISGPENVWIRKSSRKLPHRNASAAAKKKLLNVYKEDDTTINSESEKELEDINRKMLFLRGFRSWKENAQ

Target ORF information:

RefSeq Version	NM_018963.5
Organism	Homo sapiens(human)
Definition	Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018963.5

ORF Insert Sequence:

ATGGCGGAGC CGTCGTCCGC CCGACGCCCG GTGCCTCTCA TCGAGTCGGA GCTGTACTTC 
CTTATCGCCC GGTACCTATC GGCGGGCCCG TGTCGGAGAG CGGCCCAGGT GCTGGTGCAG 
GAGCTGGAGC AGTACCAGTT GTTGCCGAAG AGATTGGACT GGGAGGGCAA CGAGCACAAC 
AGGAGCTACG AGGAGTTGGT CTTGTCCAAT AAGCATGTGG CTCCTGATCA TCTTTTGCAA 
ATCTGCCAGC GCATCGGTCC TATGTTGGAT AAAGAAATTC CACCCAGTAT TTCAAGAGTC 
ACTTCTTTAC TTGGTGCAGG AAGGCAGTCT TTGCTACGTA CAGCAAAAGA CTGCAGGCAC 
ACAGTTTGGA AGGGCTCTGC CTTTGCTGCT CTTCATAGAG GAAGACCTCC TGAAATGCCA 
GTGAATTATG GTTCCCCACC AAATCTTGTG GAGATACATC GAGGAAAACA ACTCACAGGG 
TGTTCCACTT TTAGTACAGC ATTTCCAGGA ACTATGTATC AGCATATAAA AATGCACAGA 
AGGATTCTCG GACATCTATC TGCTGTTTAC TGTGTAGCAT TTGATAGGAC AGGACATAGA 
ATCTTTACAG GTTCAGATGA CTGTTTGGTA AAGATTTGGT CAACACATAA TGGCCGCTTG 
TTATCTACAT TAAGAGGTCA TTCTGCAGAA ATTTCAGATA TGGCAGTAAA CTATGAGAAT 
ACAATGATTG CTGCGGGGAG CTGTGATAAA ATTATTAGAG TGTGGTGCTT GAGAACTTGT 
GCCCCAGTTG CTGTGCTCCA AGGACACACA GGATCAATTA CATCTTTACA GTTTAGCCCG 
ATGGCCAAAG GCTCTCAAAG ATACATGGTT TCCACTGGTG CTGATGGGAC AGTTTGCTTT 
TGGCAATGGG ATTTAGAATC CTTAAAATTT AGCCCACGTC CCCTGAAGTT CACTGAAAAG 
CCTAGGCCAG GCGTTCAAAT GCTTTGTTCT TCTTTTAGTG TTGGTGGTAT GTTTTTAGCC 
ACAGGTAGTA CTGATCATGT AATCAGAATG TATTTTTTGG GTTTTGAAGC ACCCGAAAAA 
ATCGCAGAAC TTGAAAGCCA CACTGATAAA GTAGATAGTA TCCAATTTTG TAACAATGGT 
GATCGGTTCC TAAGTGGTAG CAGAGATGGA ACAGCACGGA TTTGGAGATT TGAGCAGTTA 
GAATGGAGGA GCATTTTATT GGATATGGCT ACCAGAATCT CAGGGGACTT ATCTTCCGAA 
GAGGAAAGGT TTATGAAACC TAAAGTAACA ATGATAGCTT GGAATCAAAA TGATAGCATT 
GTTGTCACAG CTGTGAATGA TCATGTCCTC AAAGTGTGGA ATTCTTACAC TGGACAACTG 
CTTCATAACT TAATGGGACA TGCTGATGAA GTATTTGTTC TGGAGACACA TCCCTTTGAT 
TCCAGAATTA TGTTATCTGC AGGACATGAT GGCAGCATAT TTATATGGGA TATTACAAAA 
GGTACCAAGA TGAAACATTA TTTTAATATG ATTGAAGGAC AAGGACATGG AGCTGTGTTT 
GACTGTAAGT TTTCACAGGA TGGACAGCAT TTTGCCTGTA CAGATTCTCA TGGGCACCTT 
CTGATATTTG GTTTTGGATG CAGCAAACCA TATGAAAAGA TTCCTGATCA GATGTTCTTC 
CATACTGACT ATCGACCACT TATTAGAGAT TCTAATAATT ATGTCTTAGA TGAGCAAACT 
CAGCAGGCTC CTCATCTTAT GCCTCCACCA TTCTTGGTAG ATGTAGATGG AAATCCTCAT 
CCAACCAAGT ATCAGAGATT AGTACCAGGC CGAGAAAATT CTGCAGATGA ACATTTGATT 
CCACAGCTGG GCTATGTGGC AACAAGTGAT GGAGAGGTGA TTGAACAAAT TATAAGCCTG 
CAAACCAATG ATAATGATGA ACGCAGCCCA GAATCGAGTA TTCTTGATGG AATGATAAGA 
CAGTTGCAGC AGCAGCAAGA TCAGAGAATG GGAGCAGATC AGGATACTAT TCCAAGAGGA 
CTTTCAAATG GTGAAGAAAC ACCCCGGAGA GGTTTTAGAA GGCTGAGCTT AGACATTCAG 
TCCCCTCCAA ATATTGGTCT GCGTCGTAGT GGACAAGTTG AAGGTGTTCG TCAGATGCAT 
CAAAACGCTC CACGCAGTCA GATTGCTACA GAACGTGACC TGCAGGCTTG GAAACGAAGA 
GTGGTTGTAC CAGAGGTACC ACTAGGCATA TTTAGGAAGC TGGAAGACTT CCGATTAGAG 
AAAGGTGAAG AGGAAAGAAA TCTTTATATA ATAGGAAGAA AAAGAAAGAC TCTTCAGCTC 
TCACATAAGT CGGATTCAGT GGTTTTGGTA TCACAGTCTA GACAAAGGAC ATGTAGGCGT 
AAATATCCAA ATTATGGTAG AAGAAATCGT AGCTGGCGTG AGTTATCTTC TGGAAATGAG 
TCTTCAAGCT CTGTAAGACA TGAGACTTCC TGTGATCAGA GTGAAGGTTC TGGTTCTTCA 
GAAGAGGATG AATGGAGAAG TGACAGAAAA AGTGAGAGTT ACAGCGAAAG TTCAAGTGAC 
TCTTCATCTA GATATTCCGA TTGGACAGCT GATGCGGGCA TCAATTTGCA GCCTCCTTTA 
AGAACATCAT GTCGTCGACG AATTACTCGA TTTTGTAGTA GTTCAGAAGA TGAAATATCT 
ACTGAGAATT TATCTCCTCC AAAAAGAAGA CGAAAGAGAA AGAAAGAAAA TAAGCCTAAG 
AAGGAGAATT TGCGGAGGAT GACTCCAGCA GAGCTTGCAA ATATGGAGCA TTTATATGAA 
TTTCACCCTC CAGTTTGGAT TACTGACACC ACACTTAGAA AATCTCCTTT TGTTCCTCAA 
ATGGGTGATG AGGTAATATA TTTTCGACAG GGTCATGAAG CTTATATTGA GGCTGTAAGA 
AGAAATAATA TTTATGAACT GAACCCTAAT AAGGAGCCAT GGAGAAAAAT GGATCTTAGG 
GATCAAGAAT TGGTTAAAAT AGTTGGAATA CGATATGAAG TTGGGCCCCC TACACTCTGT 
TGCCTAAAAC TAGCATTTAT AGATCCAGCA ACTGGAAAAC TTATGGACAA ATCTTTCTCT 
ATTAGATATC ATGATATGCC AGATGTTATT GACTTTCTTG TATTGCGTCA ATTTTATGAT 
GAAGCAAGAC AGAGGAATTG GCAGTCTTGT GACAGATTCC GCTCTATTAT TGATGATGCT 
TGGTGGTTTG GAACAGTGTT AAGTCAAGAG CCATATCAAC CACAGTATCC TGATAGTCAT 
TTCCAGTGTT ATATTGTTAG GTGGGATAAT ACTGAAATTG AAAAACTTAG CCCATGGGAC 
ATGGAACCAA TTCCTGATAA TGTTGATCCA CCTGAAGAAT TAGGAGCTAG TATTTCTGTC 
ACAACAGATG AGCTAGAGAA ATTGCTCTAT AAACCACAAG CTGGTGAATG GGGTCAGAAA 
TCAAGAGATG AAGAATGTGA TAGAATTATC AGTGGTATAG ATCAACTTTT GAATCTTGAT 
ATAGCAGCAG CTTTTGCAGG CCCTGTTGAT TTGTGTACAT ACCCGAAGTA CTGTACTGTA 
GTAGCTTATC CAACCGATCT TTACACAATT CGAATGAGAC TTGTTAATCG ATTTTACAGG 
AGGCTGTCTG CGTTAGTTTG GGAAGTCAGA TATATAGAAC ATAATGCCAG AACATTTAAC 
GAACCTGAGA GTGTAATTGC AAGATCAGCT AAAAAGATAA CTGACCAACT TTTAAAATTT 
ATCAAGAATC AACACTGTAC AAATATCTCA GAACTTTCTA ACACATCTGA AAATGATGAG 
CAAAATGCTG AGGATTTGGA TGATAGTGAT CTTCCTAAAA CATCTTCTGG AAGGAGGAGA 
GTCCATGATG GGAAAAAAAG CATCAGAGCT ACGAACTATG TTGAAAGCAA CTGGAAGAAA 
CAGTGTAAGG AACTAGTGAA CTTAATTTTT CAGTGTGAAG ATTCTGAACC ATTTAGACAA 
CCTGTTGATT TGGTTGAATA TCCAGACTAC AGAGATATTA TAGATACCCC AATGGATTTT 
GGAACAGTAA GGGAAACTCT AGATGCGGGA AATTATGACA GCCCTTTGGA GTTTTGCAAA 
GACATCCGGC TGATATTTAG CAATGCAAAA GCGTATACAC CAAACAAAAG ATCAAAGATT 
TATAGTATGA CCTTGAGATT ATCTGCCTTA TTTGAAGAAA AAATGAAGAA AATCTCTTCT 
GATTTTAAAA TTGGTCAAAA ATTCAATGAA AAACTTCGAA GAAGCCAGAG GTTCAAGCAA 
CGGCAAAATT GTAAAGGTGA CAGTCAGCCT AACAAAAGTA TCAGAAACCT CAAGCCGAAG 
AGGTTAAAAT CTCAGACAAA AATAATTCCT GAGTTGGTAG GTTCTCCTAC CCAGTCTACC 
TCAAGTAGGA CAGCTTATCT TGGAACCCAC AAGACAAGTG CTGGTATCTC TTCAGGTGTT 
ACTTCTGGTG ACTCTTCAGA TTCAGCAGAA TCATCAGAAA GGAGGAAAAG AAATAGACCT 
ATAACAAATG GTTCTACATT ATCTGAAAGT GAAGTGGAAG ATTCTTTAGC TACCTCTTTG 
TCATCGTCAG CTTCCAGTAG TTCTGAGGAA AGCAAAGAGA GTTCCAGAGC TCGTGAATCC 
TCCTCACGCA GTGGGCTATC CAGAAGCAGC AATCTCAGGG TAACCAGAAC TAGAGCTGCT 
CAAAGAAAAA CTGGTCCCGT TTCATTAGCA AATGGATGTG GCAGAAAAGC CACTCGAAAG 
AGAGTCTATT TAAGTGATTC TGATAACAAT TCATTGGAGA CTGGTGAAAT TCTAAAAGCC 
AGAGCTGGAA ATAACCGAAA AGTCTTAAGG AAGTGTGCTG CTGTGGCTGC CAATAAAATA 
AAGCTAATGA GTGATGTAGA AGAGAATTCT AGCTCTGAAA GTGTCTGTTC TGGTCGGAAG 
CTGCCTCACC GCAATGCTTC TGCTGTAGCT AGAAAAAAGT TATTACATAA TTCTGAAGAT 
GAACAGAGCT TAAAGTCAGA AATTGAAGAA GAGGAGCTAA AAGATGAAAA TCAACTATTA 
CCAGTGTCCA GTTCTCACAC TGCCCAGAGC AATGTTGATG AATCTGAAAA CAGAGACTCA 
GAGTCAGAAA GTGATTTGCG GGTAGCCCGG AAAAATTGGC ATGCTAATGG TTACAAGTCC 
CATACTCCAG CACCTTCAAA GACAAAATTT CTTAAAATAG AGTCTTCTGA GGAAGACTCT 
AAAAGTCATG ATTCAGATCA TGCATGTAAC AGAACTGCTG GCCCATCAAC GTCTGTGCAG 
AAACTTAAGG CAGAGAGCAT CTCAGAGGAA GCAGATTCTG AACCAGGAAG ATCTGGTGGT 
AGGAAATACA ATACATTTCA CAAGAATGCG AGTTTCTTTA AAAAAACCAA GATTCTGAGT 
GACTCAGAAG ACTCTGAATC TGAAGAGCAA GATAGAGAAG ATGGGAAATG TCATAAAATG 
GAAATGAACC CAATTTCAGG AAATCTGAAC TGTGACCCTA TTGCTATGTC CCAGTGTTCC 
TCAGATCATG GATGTGAAAC TGATTTAGAT TCAGATGATG ACAAAATAGA AAAACCAAAC 
AATTTTATGA AAGATTCTGC ATCACAAGAC AATGGACTAA GCAGAAAAAT TTCCAGGAAA 
AGGGTCTGTT CCAGTGACTC AGACAGTAGT TTACAGGTGG TTAAGAAATC ATCAAAAGCC 
AGAACAGGTC TCCTGAGGAT TACTCGAAGA TGTGCAGCTA CGGCTGCCAA TAAGATCAAG 
CTCATGAGTG ATGTAGAAGA TGTCAGTTTA GAAAATGTGC ACACTAGAAG CAAAAATGGA 
AGGAAAAAAC CTCTCCATCT TGCTTGTACT ACAGCTAAGA AGAAATTGAG TGATTGTGAA 
GGAAGTGTAC ATTGTGAAGT ACCAAGTGAA CAGTATGCCT GTGAAGGCAA GCCACCTGAT 
CCTGACTCCG AAGGTAGTAC AAAAGTGCTT AGTCAGGCTC TAAATGGAGA CTCAGACTCT 
GAAGATATGT TGAATTCAGA ACACAAGCAC AGGCATACCA ATATTCACAA AATAGATGCA 
CCTTCTAAAA GAAAAAGTTC CTCTGTTACA TCTTCAGGAG AAGATTCAAA AAGTCATATT 
CCAGGGAGTG AGACTGATAG GACATTTTCT TCAGAGTCAA CCTTGGCACA AAAAGCTACT 
GCAGAGAATA ATTTTGAAGT GGAACTGAAT TATGGGCTGC GCAGGTGGAA TGGCAGAAGA 
CTCAGGACCT ATGGAAAGGC TCCTTTTAGT AAGACAAAAG TGATTCATGA TTCACAGGAA 
ACAGCAGAGA AGGAAGTAAA AAGGAAGAGA TCGCATCCTG AATTGGAAAA TGTGAAAATC 
TCTGAAACAA CTGGGAATTC AAAGTTTAGA CCTGATACTA GTTCCAAATC ATCAGATTTG 
GGATCTGTAA CTGAATCAGA TATTGACTGT ACTGATAATA CAAAAACCAA AAGGAGGAAA 
ACGAAAGGAA AAGCAAAAGT AGTTAGAAAA GAATTTGTTC CTAGAGACAG AGAACCCAAT 
ACAAAAGTGA GAACATGTAT GCATAATCAG AAGGATGCAG TGCAGATGCC TAGTGAAACT 
CTGAAAGCAA AAATGGTACC TGAGAAAGTT CCCCGCAGAT GTGCTACTGT TGCTGCAAAT 
AAAATAAAGA TAATGAGTAA TCTAAAAGAA ACGATCTCAG GACCAGAAAA TGTCTGGATT 
AGGAAGAGTA GCAGAAAACT GCCCCATCGA AATGCTTCTG CTGCGGCTAA GAAAAAATTA 
CTGAATGTTT ATAAAGAGGA TGATACAACC ATAAATTCTG AAAGTGAAAA AGAGCTAGAA 
GATATTAATA GAAAAATGCT TTTTTTAAGG GGGTTCAGAT CCTGGAAAGA AAATGCACAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRWD1 ( NM_033656.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_387505.1 Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant 2, mRNA.

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CloneID	OHu22024
Clone ID Related Accession (Same CDS sequence)	NM_033656.4 , NM_033656.3
Accession Version	NM_033656.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	bromodomain and WD repeat-containing protein 1 isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA792365.1, AJ238214.2, AK125906.1 and AF129408.4. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_033656.3. Transcript Variant: This variant (2) has an alternate 3' sequence, as compared to variant 1. The encoded isoform B has a shorter and distinct C-terminus, as compared to isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ238214.2, AB080586.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGC CGTCGTCCGC CCGACGCCCG GTGCCTCTCA TCGAGTCGGA GCTGTACTTC 
CTTATCGCCC GGTACCTATC GGCGGGCCCG TGTCGGAGAG CGGCCCAGGT GCTGGTGCAG 
GAGCTGGAGC AGTACCAGTT GTTGCCGAAG AGATTGGACT GGGAGGGCAA CGAGCACAAC 
AGGAGCTACG AGGAGTTGGT CTTGTCCAAT AAGCATGTGG CTCCTGATCA TCTTTTGCAA 
ATCTGCCAGC GCATCGGTCC TATGTTGGAT AAAGAAATTC CACCCAGTAT TTCAAGAGTC 
ACTTCTTTAC TTGGTGCAGG AAGGCAGTCT TTGCTACGTA CAGCAAAAGA CTGCAGGCAC 
ACAGTTTGGA AGGGCTCTGC CTTTGCTGCT CTTCATAGAG GAAGACCTCC TGAAATGCCA 
GTGAATTATG GTTCCCCACC AAATCTTGTG GAGATACATC GAGGAAAACA ACTCACAGGG 
TGTTCCACTT TTAGTACAGC ATTTCCAGGA ACTATGTATC AGCATATAAA AATGCACAGA 
AGGATTCTCG GACATCTATC TGCTGTTTAC TGTGTAGCAT TTGATAGGAC AGGACATAGA 
ATCTTTACAG GTTCAGATGA CTGTTTGGTA AAGATTTGGT CAACACATAA TGGCCGCTTG 
TTATCTACAT TAAGAGGTCA TTCTGCAGAA ATTTCAGATA TGGCAGTAAA CTATGAGAAT 
ACAATGATTG CTGCGGGGAG CTGTGATAAA ATTATTAGAG TGTGGTGCTT GAGAACTTGT 
GCCCCAGTTG CTGTGCTCCA AGGACACACA GGATCAATTA CATCTTTACA GTTTAGCCCG 
ATGGCCAAAG GCTCTCAAAG ATACATGGTT TCCACTGGTG CTGATGGGAC AGTTTGCTTT 
TGGCAATGGG ATTTAGAATC CTTAAAATTT AGCCCACGTC CCCTGAAGTT CACTGAAAAG 
CCTAGGCCAG GCGTTCAAAT GCTTTGTTCT TCTTTTAGTG TTGGTGGTAT GTTTTTAGCC 
ACAGGTAGTA CTGATCATGT AATCAGAATG TATTTTTTGG GTTTTGAAGC ACCCGAAAAA 
ATCGCAGAAC TTGAAAGCCA CACTGATAAA GTAGATAGTA TCCAATTTTG TAACAATGGT 
GATCGGTTCC TAAGTGGTAG CAGAGATGGA ACAGCACGGA TTTGGAGATT TGAGCAGTTA 
GAATGGAGGA GCATTTTATT GGATATGGCT ACCAGAATCT CAGGGGACTT ATCTTCCGAA 
GAGGAAAGGT TTATGAAACC TAAAGTAACA ATGATAGCTT GGAATCAAAA TGATAGCATT 
GTTGTCACAG CTGTGAATGA TCATGTCCTC AAAGTGTGGA ATTCTTACAC TGGACAACTG 
CTTCATAACT TAATGGGACA TGCTGATGAA GTATTTGTTC TGGAGACACA TCCCTTTGAT 
TCCAGAATTA TGTTATCTGC AGGACATGAT GGCAGCATAT TTATATGGGA TATTACAAAA 
GGTACCAAGA TGAAACATTA TTTTAATATG ATTGAAGGAC AAGGACATGG AGCTGTGTTT 
GACTGTAAGT TTTCACAGGA TGGACAGCAT TTTGCCTGTA CAGATTCTCA TGGGCACCTT 
CTGATATTTG GTTTTGGATG CAGCAAACCA TATGAAAAGA TTCCTGATCA GATGTTCTTC 
CATACTGACT ATCGACCACT TATTAGAGAT TCTAATAATT ATGTCTTAGA TGAGCAAACT 
CAGCAGGCTC CTCATCTTAT GCCTCCACCA TTCTTGGTAG ATGTAGATGG AAATCCTCAT 
CCAACCAAGT ATCAGAGATT AGTACCAGGC CGAGAAAATT CTGCAGATGA ACATTTGATT 
CCACAGCTGG GCTATGTGGC AACAAGTGAT GGAGAGGTGA TTGAACAAAT TATAAGCCTG 
CAAACCAATG ATAATGATGA ACGCAGCCCA GAATCGAGTA TTCTTGATGG AATGATAAGA 
CAGTTGCAGC AGCAGCAAGA TCAGAGAATG GGAGCAGATC AGGATACTAT TCCAAGAGGA 
CTTTCAAATG GTGAAGAAAC ACCCCGGAGA GGTTTTAGAA GGCTGAGCTT AGACATTCAG 
TCCCCTCCAA ATATTGGTCT GCGTCGTAGT GGACAAGTTG AAGGTGTTCG TCAGATGCAT 
CAAAACGCTC CACGCAGTCA GATTGCTACA GAACGTGACC TGCAGGCTTG GAAACGAAGA 
GTGGTTGTAC CAGAGGTACC ACTAGGCATA TTTAGGAAGC TGGAAGACTT CCGATTAGAG 
AAAGGTGAAG AGGAAAGAAA TCTTTATATA ATAGGAAGAA AAAGAAAGAC TCTTCAGCTC 
TCACATAAGT CGGATTCAGT GGTTTTGGTA TCACAGTCTA GACAAAGGAC ATGTAGGCGT 
AAATATCCAA ATTATGGTAG AAGAAATCGT AGCTGGCGTG AGTTATCTTC TGGAAATGAG 
TCTTCAAGCT CTGTAAGACA TGAGACTTCC TGTGATCAGA GTGAAGGTTC TGGTTCTTCA 
GAAGAGGATG AATGGAGAAG TGACAGAAAA AGTGAGAGTT ACAGCGAAAG TTCAAGTGAC 
TCTTCATCTA GATATTCCGA TTGGACAGCT GATGCGGGCA TCAATTTGCA GCCTCCTTTA 
AGAACATCAT GTCGTCGACG AATTACTCGA TTTTGTAGTA GTTCAGAAGA TGAAATATCT 
ACTGAGAATT TATCTCCTCC AAAAAGAAGA CGAAAGAGAA AGAAAGAAAA TAAGCCTAAG 
AAGGAGAATT TGCGGAGGAT GACTCCAGCA GAGCTTGCAA ATATGGAGCA TTTATATGAA 
TTTCACCCTC CAGTTTGGAT TACTGACACC ACACTTAGAA AATCTCCTTT TGTTCCTCAA 
ATGGGTGATG AGGTAATATA TTTTCGACAG GGTCATGAAG CTTATATTGA GGCTGTAAGA 
AGAAATAATA TTTATGAACT GAACCCTAAT AAGGAGCCAT GGAGAAAAAT GGATCTTAGG 
GATCAAGAAT TGGTTAAAAT AGTTGGAATA CGATATGAAG TTGGGCCCCC TACACTCTGT 
TGCCTAAAAC TAGCATTTAT AGATCCAGCA ACTGGAAAAC TTATGGACAA ATCTTTCTCT 
ATTAGATATC ATGATATGCC AGATGTTATT GACTTTCTTG TATTGCGTCA ATTTTATGAT 
GAAGCAAGAC AGAGGAATTG GCAGTCTTGT GACAGATTCC GCTCTATTAT TGATGATGCT 
TGGTGGTTTG GAACAGTGTT AAGTCAAGAG CCATATCAAC CACAGTATCC TGATAGTCAT 
TTCCAGTGTT ATATTGTTAG GTGGGATAAT ACTGAAATTG AAAAACTTAG CCCATGGGAC 
ATGGAACCAA TTCCTGATAA TGTTGATCCA CCTGAAGAAT TAGGAGCTAG TATTTCTGTC 
ACAACAGATG AGCTAGAGAA ATTGCTCTAT AAACCACAAG CTGGTGAATG GGGTCAGAAA 
TCAAGAGATG AAGAATGTGA TAGAATTATC AGTGGTATAG ATCAACTTTT GAATCTTGAT 
ATAGCAGCAG CTTTTGCAGG CCCTGTTGAT TTGTGTACAT ACCCGAAGTA CTGTACTGTA 
GTAGCTTATC CAACCGATCT TTACACAATT CGAATGAGAC TTGTTAATCG ATTTTACAGG 
AGGCTGTCTG CGTTAGTTTG GGAAGTCAGA TATATAGAAC ATAATGCCAG AACATTTAAC 
GAACCTGAGA GTGTAATTGC AAGATCAGCT AAAAAGATAA CTGACCAACT TTTAAAATTT 
ATCAAGAATC AACACTGTAC AAATATCTCA GAACTTTCTA ACACATCTGA AAATGATGAG 
CAAAATGCTG AGGATTTGGA TGATAGTGAT CTTCCTAAAA CATCTTCTGG AAGGAGGAGA 
GTCCATGATG GGAAAAAAAG CATCAGAGCT ACGAACTATG TTGAAAGCAA CTGGAAGAAA 
CAGTGTAAGG AACTAGTGAA CTTAATTTTT CAGTGTGAAG ATTCTGAACC ATTTAGACAA 
CCTGTTGATT TGGTTGAATA TCCAGACTAC AGAGATATTA TAGATACCCC AATGGATTTT 
GGAACAGTAA GGGAAACTCT AGATGCGGGA AATTATGACA GCCCTTTGGA GTTTTGCAAA 
GACATCCGGC TGATATTTAG CAATGCAAAA GCGTATACAC CAAACAAAAG ATCAAAGATT 
TATAGTATGA CCTTGAGATT ATCTGCCTTA TTTGAAGAAA AAATGAAGAA AATCTCTTCT 
GATTTTAAAA TTGGTCAAAA ATTCAATGAA AAACTTCGAA GAAGCCAGAG GTTCAAGCAA 
CGGCAAAATT GTAAAGGTGA CAGTCAGCCT AACAAAAGTA TCAGAAACCT CAAGCCGAAG 
AGGTTAAAAT CTCAGACAAA AATAATTCCT GAGTTGGTAG GTTCTCCTAC CCAGTCTACC 
TCAAGTAGGA CAGCTTATCT TGGAACCCAC AAGACAAGTG CTGGTATCTC TTCAGGTGTT 
ACTTCTGGTG ACTCTTCAGA TTCAGCAGAA TCATCAGAAA GGAGGAAAAG AAATAGACCT 
ATAACAAATG GTTCTACATT ATCTGAAAGT GAAGTGGAAG ATTCTTTAGC TACCTCTTTG 
TCATCGTCAG CTTCCAGTAG TTCTGAGGAA AGCAAAGAGA GTTCCAGAGC TCGTGAATCC 
TCCTCACGCA GTGGGCTATC CAGAAGCAGC AATCTCAGGG TAACCAGAAC TAGAGCTGCT 
CAAAGAAAAA CTGGTCCCGT TTCATTAGCA AATGGATGTG GCAGAAAAGC CACTCGAAAG 
AGAGTCTATT TAAGTGATTC TGATAACAAT TCATTGGAGA CTGGTGAAAT TCTAAAAGCC 
AGAGCTGGAA ATAACCGAAA AGTCTTAAGG AAGTGTGCTG CTGTGGCTGC CAATAAAATA 
AAGCTAATGA GTGATGTAGA AGAGAATTCT AGCTCTGAAA GTGTCTGTTC TGGTCGGAAG 
CTGCCTCACC GCAATGCTTC TGCTGTAGCT AGAAAAAAGT TATTACATAA TTCTGAAGAT 
GAACAGAGCT TAAAGTCAGA AATTGAAGAA GAGGAGCTAA AAGATGAAAA TCAACTATTA 
CCAGTGTCCA GTTCTCACAC TGCCCAGAGC AATGTTGATG AATCTGAAAA CAGAGACTCA 
GAGTCAGAAA GTGATTTGCG GGTAGCCCGG AAAAATTGGC ATGCTAATGG TTACAAGTCC 
CATACTCCAG CACCTTCAAA GACAAAATTT CTTAAAATAG AGTCTTCTGA GGAAGACTCT 
AAAAGTCATG ATTCAGATCA TGCATGTAAC AGAACTGCTG GCCCATCAAC GTCTGTGCAG 
AAACTTAAGG CAGAGAGCAT CTCAGAGGAA GCAGATTCTG AACCAGGAAG ATCTGGTGGT 
AGGAAATACA ATACATTTCA CAAGAATGCG AGTTTCTTTA AAAAAACCAA GATTCTGAGT 
GACTCAGAAG ACTCTGAATC TGAAGAGCAA GATAGAGAAG ATGGGAAATG TCATAAAATG 
GAAATGAACC CAATTTCAGG AAATCTGAAC TGTGACCCTA TTGCTATGTC CCAGTGTTCC 
TCAGATCATG GATGTGAAAC TGATTTAGAT TCAGATGATG ACAAAATAGA AAAACCAAAC 
AATTTTATGA AAGATTCTGC ATCACAAGAC AATGGACTAA GCAGAAAAAT TTCCAGGAAA 
AGGGTCTGTT CCAGTGACTC AGACAGTAGT TTACAGGTGG TTAAGAAATC ATCAAAAGCC 
AGAACAGGTC TCCTGAGGAT TACTCGAAGA TGTGCAGCTA CGGCTGCCAA TAAGATCAAG 
CTCATGAGTG ATGTAGAAGA TGTCAGTTTA GAAAATGTGC ACACTAGAAG CAAAAATGGA 
AGGAAAAAAC CTCTCCATCT TGCTTGTACT ACAGCTAAGA AGAAATTGAG TGATTGTGAA 
GGAAGTGTAC ATTGTGAAGT ACCAAGTGAA CAGTATGCCT GTGAAGGCAA GCCACCTGAT 
CCTGACTCCG AAGGTAGTAC AAAAGTGCTT AGTCAGGCTC TAAATGGAGA CTCAGACTCT 
GAAGATATGT TGAATTCAGA ACACAAGCAC AGGCATACCA ATATTCACAA AATAGATGCA 
CCTTCTAAAA GAAAAAGTTC CTCTGTTACA TCTTCAGGAG AAGATTCAAA AAGTCATATT 
CCAGGGAGTG AGACTGATAG GACATTTTCT TCAGAGTCAA CCTTGGCACA AAAAGCTACT 
GCAGAGAATA ATTTTGAAGT GGAACTGAAT TATGGGCTGC GCAGGTGGAA TGGCAGAAGA 
CTCAGGACCT ATGGAAAGGC TCCTTTTAGT AAGACAAAAG TGATTCATGA TTCACAGGAA 
ACAGCAGAGA AGGAAGTAAA AAGGAAGAGA TCGCATCCTG AATTGGAAAA TGTGAAAATC 
TCTGAAACAA CTGGGAATTC AAAGTTTAGA CCTGATACTA GTTCCAAATC ATCAGATTTG 
GGATCTGTAA CTGAATCAGA TATTGACTGT ACTGATAATA CAAAAACCAA AAGGAGGAAA 
ACGAAAGGAA AAGCAAAAGT AGTTAGAAAA GGTAAAACTT TTACAGCTAA CATATCTAAA 
ACTGTGAGAC GTCAAAGACA AAGCAAACGC CCTAGGTTAA GTGTGGATGA TAATGACTGG 
GAGGATTTGG ACTATGCAAA ATCTAAAAGA GTTCTTCGAC GTTCAAAAAT AAAAACGAGA 
AATCAGGGTA GAAGGACTGT GAGATACCAT GATGGGGATG ATGACAGAAG TTTAGAAAAT 
GTGTTAGATT TCAATGGTTG CACCTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_387505.1
CDS	142..6951
Translation	MAEPSSARRPVPLIESELYFLIARYLSAGPCRRAAQVLVQELEQYQLLPKRLDWEGNEHNRSYEELVLSNKHVAPDHLLQICQRIGPMLDKEIPPSISRVTSLLGAGRQSLLRTAKDCRHTVWKGSAFAALHRGRPPEMPVNYGSPPNLVEIHRGKQLTGCSTFSTAFPGTMYQHIKMHRRILGHLSAVYCVAFDRTGHRIFTGSDDCLVKIWSTHNGRLLSTLRGHSAEISDMAVNYENTMIAAGSCDKIIRVWCLRTCAPVAVLQGHTGSITSLQFSPMAKGSQRYMVSTGADGTVCFWQWDLESLKFSPRPLKFTEKPRPGVQMLCSSFSVGGMFLATGSTDHVIRMYFLGFEAPEKIAELESHTDKVDSIQFCNNGDRFLSGSRDGTARIWRFEQLEWRSILLDMATRISGDLSSEEERFMKPKVTMIAWNQNDSIVVTAVNDHVLKVWNSYTGQLLHNLMGHADEVFVLETHPFDSRIMLSAGHDGSIFIWDITKGTKMKHYFNMIEGQGHGAVFDCKFSQDGQHFACTDSHGHLLIFGFGCSKPYEKIPDQMFFHTDYRPLIRDSNNYVLDEQTQQAPHLMPPPFLVDVDGNPHPTKYQRLVPGRENSADEHLIPQLGYVATSDGEVIEQIISLQTNDNDERSPESSILDGMIRQLQQQQDQRMGADQDTIPRGLSNGEETPRRGFRRLSLDIQSPPNIGLRRSGQVEGVRQMHQNAPRSQIATERDLQAWKRRVVVPEVPLGIFRKLEDFRLEKGEEERNLYIIGRKRKTLQLSHKSDSVVLVSQSRQRTCRRKYPNYGRRNRSWRELSSGNESSSSVRHETSCDQSEGSGSSEEDEWRSDRKSESYSESSSDSSSRYSDWTADAGINLQPPLRTSCRRRITRFCSSSEDEISTENLSPPKRRRKRKKENKPKKENLRRMTPAELANMEHLYEFHPPVWITDTTLRKSPFVPQMGDEVIYFRQGHEAYIEAVRRNNIYELNPNKEPWRKMDLRDQELVKIVGIRYEVGPPTLCCLKLAFIDPATGKLMDKSFSIRYHDMPDVIDFLVLRQFYDEARQRNWQSCDRFRSIIDDAWWFGTVLSQEPYQPQYPDSHFQCYIVRWDNTEIEKLSPWDMEPIPDNVDPPEELGASISVTTDELEKLLYKPQAGEWGQKSRDEECDRIISGIDQLLNLDIAAAFAGPVDLCTYPKYCTVVAYPTDLYTIRMRLVNRFYRRLSALVWEVRYIEHNARTFNEPESVIARSAKKITDQLLKFIKNQHCTNISELSNTSENDEQNAEDLDDSDLPKTSSGRRRVHDGKKSIRATNYVESNWKKQCKELVNLIFQCEDSEPFRQPVDLVEYPDYRDIIDTPMDFGTVRETLDAGNYDSPLEFCKDIRLIFSNAKAYTPNKRSKIYSMTLRLSALFEEKMKKISSDFKIGQKFNEKLRRSQRFKQRQNCKGDSQPNKSIRNLKPKRLKSQTKIIPELVGSPTQSTSSRTAYLGTHKTSAGISSGVTSGDSSDSAESSERRKRNRPITNGSTLSESEVEDSLATSLSSSASSSSEESKESSRARESSSRSGLSRSSNLRVTRTRAAQRKTGPVSLANGCGRKATRKRVYLSDSDNNSLETGEILKARAGNNRKVLRKCAAVAANKIKLMSDVEENSSSESVCSGRKLPHRNASAVARKKLLHNSEDEQSLKSEIEEEELKDENQLLPVSSSHTAQSNVDESENRDSESESDLRVARKNWHANGYKSHTPAPSKTKFLKIESSEEDSKSHDSDHACNRTAGPSTSVQKLKAESISEEADSEPGRSGGRKYNTFHKNASFFKKTKILSDSEDSESEEQDREDGKCHKMEMNPISGNLNCDPIAMSQCSSDHGCETDLDSDDDKIEKPNNFMKDSASQDNGLSRKISRKRVCSSDSDSSLQVVKKSSKARTGLLRITRRCAATAANKIKLMSDVEDVSLENVHTRSKNGRKKPLHLACTTAKKKLSDCEGSVHCEVPSEQYACEGKPPDPDSEGSTKVLSQALNGDSDSEDMLNSEHKHRHTNIHKIDAPSKRKSSSVTSSGEDSKSHIPGSETDRTFSSESTLAQKATAENNFEVELNYGLRRWNGRRLRTYGKAPFSKTKVIHDSQETAEKEVKRKRSHPELENVKISETTGNSKFRPDTSSKSSDLGSVTESDIDCTDNTKTKRRKTKGKAKVVRKGKTFTANISKTVRRQRQSKRPRLSVDDNDWEDLDYAKSKRVLRRSKIKTRNQGRRTVRYHDGDDDRSLENVLDFNGCTL

Target ORF information:

RefSeq Version	NM_033656.4
Organism	Homo sapiens(human)
Definition	Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033656.4

ORF Insert Sequence:

ATGGCGGAGC CGTCGTCCGC CCGACGCCCG GTGCCTCTCA TCGAGTCGGA GCTGTACTTC 
CTTATCGCCC GGTACCTATC GGCGGGCCCG TGTCGGAGAG CGGCCCAGGT GCTGGTGCAG 
GAGCTGGAGC AGTACCAGTT GTTGCCGAAG AGATTGGACT GGGAGGGCAA CGAGCACAAC 
AGGAGCTACG AGGAGTTGGT CTTGTCCAAT AAGCATGTGG CTCCTGATCA TCTTTTGCAA 
ATCTGCCAGC GCATCGGTCC TATGTTGGAT AAAGAAATTC CACCCAGTAT TTCAAGAGTC 
ACTTCTTTAC TTGGTGCAGG AAGGCAGTCT TTGCTACGTA CAGCAAAAGA CTGCAGGCAC 
ACAGTTTGGA AGGGCTCTGC CTTTGCTGCT CTTCATAGAG GAAGACCTCC TGAAATGCCA 
GTGAATTATG GTTCCCCACC AAATCTTGTG GAGATACATC GAGGAAAACA ACTCACAGGG 
TGTTCCACTT TTAGTACAGC ATTTCCAGGA ACTATGTATC AGCATATAAA AATGCACAGA 
AGGATTCTCG GACATCTATC TGCTGTTTAC TGTGTAGCAT TTGATAGGAC AGGACATAGA 
ATCTTTACAG GTTCAGATGA CTGTTTGGTA AAGATTTGGT CAACACATAA TGGCCGCTTG 
TTATCTACAT TAAGAGGTCA TTCTGCAGAA ATTTCAGATA TGGCAGTAAA CTATGAGAAT 
ACAATGATTG CTGCGGGGAG CTGTGATAAA ATTATTAGAG TGTGGTGCTT GAGAACTTGT 
GCCCCAGTTG CTGTGCTCCA AGGACACACA GGATCAATTA CATCTTTACA GTTTAGCCCG 
ATGGCCAAAG GCTCTCAAAG ATACATGGTT TCCACTGGTG CTGATGGGAC AGTTTGCTTT 
TGGCAATGGG ATTTAGAATC CTTAAAATTT AGCCCACGTC CCCTGAAGTT CACTGAAAAG 
CCTAGGCCAG GCGTTCAAAT GCTTTGTTCT TCTTTTAGTG TTGGTGGTAT GTTTTTAGCC 
ACAGGTAGTA CTGATCATGT AATCAGAATG TATTTTTTGG GTTTTGAAGC ACCCGAAAAA 
ATCGCAGAAC TTGAAAGCCA CACTGATAAA GTAGATAGTA TCCAATTTTG TAACAATGGT 
GATCGGTTCC TAAGTGGTAG CAGAGATGGA ACAGCACGGA TTTGGAGATT TGAGCAGTTA 
GAATGGAGGA GCATTTTATT GGATATGGCT ACCAGAATCT CAGGGGACTT ATCTTCCGAA 
GAGGAAAGGT TTATGAAACC TAAAGTAACA ATGATAGCTT GGAATCAAAA TGATAGCATT 
GTTGTCACAG CTGTGAATGA TCATGTCCTC AAAGTGTGGA ATTCTTACAC TGGACAACTG 
CTTCATAACT TAATGGGACA TGCTGATGAA GTATTTGTTC TGGAGACACA TCCCTTTGAT 
TCCAGAATTA TGTTATCTGC AGGACATGAT GGCAGCATAT TTATATGGGA TATTACAAAA 
GGTACCAAGA TGAAACATTA TTTTAATATG ATTGAAGGAC AAGGACATGG AGCTGTGTTT 
GACTGTAAGT TTTCACAGGA TGGACAGCAT TTTGCCTGTA CAGATTCTCA TGGGCACCTT 
CTGATATTTG GTTTTGGATG CAGCAAACCA TATGAAAAGA TTCCTGATCA GATGTTCTTC 
CATACTGACT ATCGACCACT TATTAGAGAT TCTAATAATT ATGTCTTAGA TGAGCAAACT 
CAGCAGGCTC CTCATCTTAT GCCTCCACCA TTCTTGGTAG ATGTAGATGG AAATCCTCAT 
CCAACCAAGT ATCAGAGATT AGTACCAGGC CGAGAAAATT CTGCAGATGA ACATTTGATT 
CCACAGCTGG GCTATGTGGC AACAAGTGAT GGAGAGGTGA TTGAACAAAT TATAAGCCTG 
CAAACCAATG ATAATGATGA ACGCAGCCCA GAATCGAGTA TTCTTGATGG AATGATAAGA 
CAGTTGCAGC AGCAGCAAGA TCAGAGAATG GGAGCAGATC AGGATACTAT TCCAAGAGGA 
CTTTCAAATG GTGAAGAAAC ACCCCGGAGA GGTTTTAGAA GGCTGAGCTT AGACATTCAG 
TCCCCTCCAA ATATTGGTCT GCGTCGTAGT GGACAAGTTG AAGGTGTTCG TCAGATGCAT 
CAAAACGCTC CACGCAGTCA GATTGCTACA GAACGTGACC TGCAGGCTTG GAAACGAAGA 
GTGGTTGTAC CAGAGGTACC ACTAGGCATA TTTAGGAAGC TGGAAGACTT CCGATTAGAG 
AAAGGTGAAG AGGAAAGAAA TCTTTATATA ATAGGAAGAA AAAGAAAGAC TCTTCAGCTC 
TCACATAAGT CGGATTCAGT GGTTTTGGTA TCACAGTCTA GACAAAGGAC ATGTAGGCGT 
AAATATCCAA ATTATGGTAG AAGAAATCGT AGCTGGCGTG AGTTATCTTC TGGAAATGAG 
TCTTCAAGCT CTGTAAGACA TGAGACTTCC TGTGATCAGA GTGAAGGTTC TGGTTCTTCA 
GAAGAGGATG AATGGAGAAG TGACAGAAAA AGTGAGAGTT ACAGCGAAAG TTCAAGTGAC 
TCTTCATCTA GATATTCCGA TTGGACAGCT GATGCGGGCA TCAATTTGCA GCCTCCTTTA 
AGAACATCAT GTCGTCGACG AATTACTCGA TTTTGTAGTA GTTCAGAAGA TGAAATATCT 
ACTGAGAATT TATCTCCTCC AAAAAGAAGA CGAAAGAGAA AGAAAGAAAA TAAGCCTAAG 
AAGGAGAATT TGCGGAGGAT GACTCCAGCA GAGCTTGCAA ATATGGAGCA TTTATATGAA 
TTTCACCCTC CAGTTTGGAT TACTGACACC ACACTTAGAA AATCTCCTTT TGTTCCTCAA 
ATGGGTGATG AGGTAATATA TTTTCGACAG GGTCATGAAG CTTATATTGA GGCTGTAAGA 
AGAAATAATA TTTATGAACT GAACCCTAAT AAGGAGCCAT GGAGAAAAAT GGATCTTAGG 
GATCAAGAAT TGGTTAAAAT AGTTGGAATA CGATATGAAG TTGGGCCCCC TACACTCTGT 
TGCCTAAAAC TAGCATTTAT AGATCCAGCA ACTGGAAAAC TTATGGACAA ATCTTTCTCT 
ATTAGATATC ATGATATGCC AGATGTTATT GACTTTCTTG TATTGCGTCA ATTTTATGAT 
GAAGCAAGAC AGAGGAATTG GCAGTCTTGT GACAGATTCC GCTCTATTAT TGATGATGCT 
TGGTGGTTTG GAACAGTGTT AAGTCAAGAG CCATATCAAC CACAGTATCC TGATAGTCAT 
TTCCAGTGTT ATATTGTTAG GTGGGATAAT ACTGAAATTG AAAAACTTAG CCCATGGGAC 
ATGGAACCAA TTCCTGATAA TGTTGATCCA CCTGAAGAAT TAGGAGCTAG TATTTCTGTC 
ACAACAGATG AGCTAGAGAA ATTGCTCTAT AAACCACAAG CTGGTGAATG GGGTCAGAAA 
TCAAGAGATG AAGAATGTGA TAGAATTATC AGTGGTATAG ATCAACTTTT GAATCTTGAT 
ATAGCAGCAG CTTTTGCAGG CCCTGTTGAT TTGTGTACAT ACCCGAAGTA CTGTACTGTA 
GTAGCTTATC CAACCGATCT TTACACAATT CGAATGAGAC TTGTTAATCG ATTTTACAGG 
AGGCTGTCTG CGTTAGTTTG GGAAGTCAGA TATATAGAAC ATAATGCCAG AACATTTAAC 
GAACCTGAGA GTGTAATTGC AAGATCAGCT AAAAAGATAA CTGACCAACT TTTAAAATTT 
ATCAAGAATC AACACTGTAC AAATATCTCA GAACTTTCTA ACACATCTGA AAATGATGAG 
CAAAATGCTG AGGATTTGGA TGATAGTGAT CTTCCTAAAA CATCTTCTGG AAGGAGGAGA 
GTCCATGATG GGAAAAAAAG CATCAGAGCT ACGAACTATG TTGAAAGCAA CTGGAAGAAA 
CAGTGTAAGG AACTAGTGAA CTTAATTTTT CAGTGTGAAG ATTCTGAACC ATTTAGACAA 
CCTGTTGATT TGGTTGAATA TCCAGACTAC AGAGATATTA TAGATACCCC AATGGATTTT 
GGAACAGTAA GGGAAACTCT AGATGCGGGA AATTATGACA GCCCTTTGGA GTTTTGCAAA 
GACATCCGGC TGATATTTAG CAATGCAAAA GCGTATACAC CAAACAAAAG ATCAAAGATT 
TATAGTATGA CCTTGAGATT ATCTGCCTTA TTTGAAGAAA AAATGAAGAA AATCTCTTCT 
GATTTTAAAA TTGGTCAAAA ATTCAATGAA AAACTTCGAA GAAGCCAGAG GTTCAAGCAA 
CGGCAAAATT GTAAAGGTGA CAGTCAGCCT AACAAAAGTA TCAGAAACCT CAAGCCGAAG 
AGGTTAAAAT CTCAGACAAA AATAATTCCT GAGTTGGTAG GTTCTCCTAC CCAGTCTACC 
TCAAGTAGGA CAGCTTATCT TGGAACCCAC AAGACAAGTG CTGGTATCTC TTCAGGTGTT 
ACTTCTGGTG ACTCTTCAGA TTCAGCAGAA TCATCAGAAA GGAGGAAAAG AAATAGACCT 
ATAACAAATG GTTCTACATT ATCTGAAAGT GAAGTGGAAG ATTCTTTAGC TACCTCTTTG 
TCATCGTCAG CTTCCAGTAG TTCTGAGGAA AGCAAAGAGA GTTCCAGAGC TCGTGAATCC 
TCCTCACGCA GTGGGCTATC CAGAAGCAGC AATCTCAGGG TAACCAGAAC TAGAGCTGCT 
CAAAGAAAAA CTGGTCCCGT TTCATTAGCA AATGGATGTG GCAGAAAAGC CACTCGAAAG 
AGAGTCTATT TAAGTGATTC TGATAACAAT TCATTGGAGA CTGGTGAAAT TCTAAAAGCC 
AGAGCTGGAA ATAACCGAAA AGTCTTAAGG AAGTGTGCTG CTGTGGCTGC CAATAAAATA 
AAGCTAATGA GTGATGTAGA AGAGAATTCT AGCTCTGAAA GTGTCTGTTC TGGTCGGAAG 
CTGCCTCACC GCAATGCTTC TGCTGTAGCT AGAAAAAAGT TATTACATAA TTCTGAAGAT 
GAACAGAGCT TAAAGTCAGA AATTGAAGAA GAGGAGCTAA AAGATGAAAA TCAACTATTA 
CCAGTGTCCA GTTCTCACAC TGCCCAGAGC AATGTTGATG AATCTGAAAA CAGAGACTCA 
GAGTCAGAAA GTGATTTGCG GGTAGCCCGG AAAAATTGGC ATGCTAATGG TTACAAGTCC 
CATACTCCAG CACCTTCAAA GACAAAATTT CTTAAAATAG AGTCTTCTGA GGAAGACTCT 
AAAAGTCATG ATTCAGATCA TGCATGTAAC AGAACTGCTG GCCCATCAAC GTCTGTGCAG 
AAACTTAAGG CAGAGAGCAT CTCAGAGGAA GCAGATTCTG AACCAGGAAG ATCTGGTGGT 
AGGAAATACA ATACATTTCA CAAGAATGCG AGTTTCTTTA AAAAAACCAA GATTCTGAGT 
GACTCAGAAG ACTCTGAATC TGAAGAGCAA GATAGAGAAG ATGGGAAATG TCATAAAATG 
GAAATGAACC CAATTTCAGG AAATCTGAAC TGTGACCCTA TTGCTATGTC CCAGTGTTCC 
TCAGATCATG GATGTGAAAC TGATTTAGAT TCAGATGATG ACAAAATAGA AAAACCAAAC 
AATTTTATGA AAGATTCTGC ATCACAAGAC AATGGACTAA GCAGAAAAAT TTCCAGGAAA 
AGGGTCTGTT CCAGTGACTC AGACAGTAGT TTACAGGTGG TTAAGAAATC ATCAAAAGCC 
AGAACAGGTC TCCTGAGGAT TACTCGAAGA TGTGCAGCTA CGGCTGCCAA TAAGATCAAG 
CTCATGAGTG ATGTAGAAGA TGTCAGTTTA GAAAATGTGC ACACTAGAAG CAAAAATGGA 
AGGAAAAAAC CTCTCCATCT TGCTTGTACT ACAGCTAAGA AGAAATTGAG TGATTGTGAA 
GGAAGTGTAC ATTGTGAAGT ACCAAGTGAA CAGTATGCCT GTGAAGGCAA GCCACCTGAT 
CCTGACTCCG AAGGTAGTAC AAAAGTGCTT AGTCAGGCTC TAAATGGAGA CTCAGACTCT 
GAAGATATGT TGAATTCAGA ACACAAGCAC AGGCATACCA ATATTCACAA AATAGATGCA 
CCTTCTAAAA GAAAAAGTTC CTCTGTTACA TCTTCAGGAG AAGATTCAAA AAGTCATATT 
CCAGGGAGTG AGACTGATAG GACATTTTCT TCAGAGTCAA CCTTGGCACA AAAAGCTACT 
GCAGAGAATA ATTTTGAAGT GGAACTGAAT TATGGGCTGC GCAGGTGGAA TGGCAGAAGA 
CTCAGGACCT ATGGAAAGGC TCCTTTTAGT AAGACAAAAG TGATTCATGA TTCACAGGAA 
ACAGCAGAGA AGGAAGTAAA AAGGAAGAGA TCGCATCCTG AATTGGAAAA TGTGAAAATC 
TCTGAAACAA CTGGGAATTC AAAGTTTAGA CCTGATACTA GTTCCAAATC ATCAGATTTG 
GGATCTGTAA CTGAATCAGA TATTGACTGT ACTGATAATA CAAAAACCAA AAGGAGGAAA 
ACGAAAGGAA AAGCAAAAGT AGTTAGAAAA GGTAAAACTT TTACAGCTAA CATATCTAAA 
ACTGTGAGAC GTCAAAGACA AAGCAAACGC CCTAGGTTAA GTGTGGATGA TAATGACTGG 
GAGGATTTGG ACTATGCAAA ATCTAAAAGA GTTCTTCGAC GTTCAAAAAT AAAAACGAGA 
AATCAGGGTA GAAGGACTGT GAGATACCAT GATGGGGATG ATGACAGAAG TTTAGAAAAT 
GTGTTAGATT TCAATGGTTG CACCTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRWD1 ( NM_033656.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_387505.1 Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant 2, mRNA.

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CloneID	OHu22024
Clone ID Related Accession (Same CDS sequence)	NM_033656.4 , NM_033656.3
Accession Version	NM_033656.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	bromodomain and WD repeat-containing protein 1 isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA792365.1, AJ238214.2, AK125906.1, AF129408.4 and AA807344.1. This sequence is a reference standard in the RefSeqGene project. On Jun 8, 2011 this sequence version replaced NM_033656.2. Transcript Variant: This variant (2) has an alternate 3' sequence, as compared to variant 1. The encoded isoform B has a shorter and distinct C-terminus, as compared to isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ238214.2, AB080586.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGC CGTCGTCCGC CCGACGCCCG GTGCCTCTCA TCGAGTCGGA GCTGTACTTC 
CTTATCGCCC GGTACCTATC GGCGGGCCCG TGTCGGAGAG CGGCCCAGGT GCTGGTGCAG 
GAGCTGGAGC AGTACCAGTT GTTGCCGAAG AGATTGGACT GGGAGGGCAA CGAGCACAAC 
AGGAGCTACG AGGAGTTGGT CTTGTCCAAT AAGCATGTGG CTCCTGATCA TCTTTTGCAA 
ATCTGCCAGC GCATCGGTCC TATGTTGGAT AAAGAAATTC CACCCAGTAT TTCAAGAGTC 
ACTTCTTTAC TTGGTGCAGG AAGGCAGTCT TTGCTACGTA CAGCAAAAGA CTGCAGGCAC 
ACAGTTTGGA AGGGCTCTGC CTTTGCTGCT CTTCATAGAG GAAGACCTCC TGAAATGCCA 
GTGAATTATG GTTCCCCACC AAATCTTGTG GAGATACATC GAGGAAAACA ACTCACAGGG 
TGTTCCACTT TTAGTACAGC ATTTCCAGGA ACTATGTATC AGCATATAAA AATGCACAGA 
AGGATTCTCG GACATCTATC TGCTGTTTAC TGTGTAGCAT TTGATAGGAC AGGACATAGA 
ATCTTTACAG GTTCAGATGA CTGTTTGGTA AAGATTTGGT CAACACATAA TGGCCGCTTG 
TTATCTACAT TAAGAGGTCA TTCTGCAGAA ATTTCAGATA TGGCAGTAAA CTATGAGAAT 
ACAATGATTG CTGCGGGGAG CTGTGATAAA ATTATTAGAG TGTGGTGCTT GAGAACTTGT 
GCCCCAGTTG CTGTGCTCCA AGGACACACA GGATCAATTA CATCTTTACA GTTTAGCCCG 
ATGGCCAAAG GCTCTCAAAG ATACATGGTT TCCACTGGTG CTGATGGGAC AGTTTGCTTT 
TGGCAATGGG ATTTAGAATC CTTAAAATTT AGCCCACGTC CCCTGAAGTT CACTGAAAAG 
CCTAGGCCAG GCGTTCAAAT GCTTTGTTCT TCTTTTAGTG TTGGTGGTAT GTTTTTAGCC 
ACAGGTAGTA CTGATCATGT AATCAGAATG TATTTTTTGG GTTTTGAAGC ACCCGAAAAA 
ATCGCAGAAC TTGAAAGCCA CACTGATAAA GTAGATAGTA TCCAATTTTG TAACAATGGT 
GATCGGTTCC TAAGTGGTAG CAGAGATGGA ACAGCACGGA TTTGGAGATT TGAGCAGTTA 
GAATGGAGGA GCATTTTATT GGATATGGCT ACCAGAATCT CAGGGGACTT ATCTTCCGAA 
GAGGAAAGGT TTATGAAACC TAAAGTAACA ATGATAGCTT GGAATCAAAA TGATAGCATT 
GTTGTCACAG CTGTGAATGA TCATGTCCTC AAAGTGTGGA ATTCTTACAC TGGACAACTG 
CTTCATAACT TAATGGGACA TGCTGATGAA GTATTTGTTC TGGAGACACA TCCCTTTGAT 
TCCAGAATTA TGTTATCTGC AGGACATGAT GGCAGCATAT TTATATGGGA TATTACAAAA 
GGTACCAAGA TGAAACATTA TTTTAATATG ATTGAAGGAC AAGGACATGG AGCTGTGTTT 
GACTGTAAGT TTTCACAGGA TGGACAGCAT TTTGCCTGTA CAGATTCTCA TGGGCACCTT 
CTGATATTTG GTTTTGGATG CAGCAAACCA TATGAAAAGA TTCCTGATCA GATGTTCTTC 
CATACTGACT ATCGACCACT TATTAGAGAT TCTAATAATT ATGTCTTAGA TGAGCAAACT 
CAGCAGGCTC CTCATCTTAT GCCTCCACCA TTCTTGGTAG ATGTAGATGG AAATCCTCAT 
CCAACCAAGT ATCAGAGATT AGTACCAGGC CGAGAAAATT CTGCAGATGA ACATTTGATT 
CCACAGCTGG GCTATGTGGC AACAAGTGAT GGAGAGGTGA TTGAACAAAT TATAAGCCTG 
CAAACCAATG ATAATGATGA ACGCAGCCCA GAATCGAGTA TTCTTGATGG AATGATAAGA 
CAGTTGCAGC AGCAGCAAGA TCAGAGAATG GGAGCAGATC AGGATACTAT TCCAAGAGGA 
CTTTCAAATG GTGAAGAAAC ACCCCGGAGA GGTTTTAGAA GGCTGAGCTT AGACATTCAG 
TCCCCTCCAA ATATTGGTCT GCGTCGTAGT GGACAAGTTG AAGGTGTTCG TCAGATGCAT 
CAAAACGCTC CACGCAGTCA GATTGCTACA GAACGTGACC TGCAGGCTTG GAAACGAAGA 
GTGGTTGTAC CAGAGGTACC ACTAGGCATA TTTAGGAAGC TGGAAGACTT CCGATTAGAG 
AAAGGTGAAG AGGAAAGAAA TCTTTATATA ATAGGAAGAA AAAGAAAGAC TCTTCAGCTC 
TCACATAAGT CGGATTCAGT GGTTTTGGTA TCACAGTCTA GACAAAGGAC ATGTAGGCGT 
AAATATCCAA ATTATGGTAG AAGAAATCGT AGCTGGCGTG AGTTATCTTC TGGAAATGAG 
TCTTCAAGCT CTGTAAGACA TGAGACTTCC TGTGATCAGA GTGAAGGTTC TGGTTCTTCA 
GAAGAGGATG AATGGAGAAG TGACAGAAAA AGTGAGAGTT ACAGCGAAAG TTCAAGTGAC 
TCTTCATCTA GATATTCCGA TTGGACAGCT GATGCGGGCA TCAATTTGCA GCCTCCTTTA 
AGAACATCAT GTCGTCGACG AATTACTCGA TTTTGTAGTA GTTCAGAAGA TGAAATATCT 
ACTGAGAATT TATCTCCTCC AAAAAGAAGA CGAAAGAGAA AGAAAGAAAA TAAGCCTAAG 
AAGGAGAATT TGCGGAGGAT GACTCCAGCA GAGCTTGCAA ATATGGAGCA TTTATATGAA 
TTTCACCCTC CAGTTTGGAT TACTGACACC ACACTTAGAA AATCTCCTTT TGTTCCTCAA 
ATGGGTGATG AGGTAATATA TTTTCGACAG GGTCATGAAG CTTATATTGA GGCTGTAAGA 
AGAAATAATA TTTATGAACT GAACCCTAAT AAGGAGCCAT GGAGAAAAAT GGATCTTAGG 
GATCAAGAAT TGGTTAAAAT AGTTGGAATA CGATATGAAG TTGGGCCCCC TACACTCTGT 
TGCCTAAAAC TAGCATTTAT AGATCCAGCA ACTGGAAAAC TTATGGACAA ATCTTTCTCT 
ATTAGATATC ATGATATGCC AGATGTTATT GACTTTCTTG TATTGCGTCA ATTTTATGAT 
GAAGCAAGAC AGAGGAATTG GCAGTCTTGT GACAGATTCC GCTCTATTAT TGATGATGCT 
TGGTGGTTTG GAACAGTGTT AAGTCAAGAG CCATATCAAC CACAGTATCC TGATAGTCAT 
TTCCAGTGTT ATATTGTTAG GTGGGATAAT ACTGAAATTG AAAAACTTAG CCCATGGGAC 
ATGGAACCAA TTCCTGATAA TGTTGATCCA CCTGAAGAAT TAGGAGCTAG TATTTCTGTC 
ACAACAGATG AGCTAGAGAA ATTGCTCTAT AAACCACAAG CTGGTGAATG GGGTCAGAAA 
TCAAGAGATG AAGAATGTGA TAGAATTATC AGTGGTATAG ATCAACTTTT GAATCTTGAT 
ATAGCAGCAG CTTTTGCAGG CCCTGTTGAT TTGTGTACAT ACCCGAAGTA CTGTACTGTA 
GTAGCTTATC CAACCGATCT TTACACAATT CGAATGAGAC TTGTTAATCG ATTTTACAGG 
AGGCTGTCTG CGTTAGTTTG GGAAGTCAGA TATATAGAAC ATAATGCCAG AACATTTAAC 
GAACCTGAGA GTGTAATTGC AAGATCAGCT AAAAAGATAA CTGACCAACT TTTAAAATTT 
ATCAAGAATC AACACTGTAC AAATATCTCA GAACTTTCTA ACACATCTGA AAATGATGAG 
CAAAATGCTG AGGATTTGGA TGATAGTGAT CTTCCTAAAA CATCTTCTGG AAGGAGGAGA 
GTCCATGATG GGAAAAAAAG CATCAGAGCT ACGAACTATG TTGAAAGCAA CTGGAAGAAA 
CAGTGTAAGG AACTAGTGAA CTTAATTTTT CAGTGTGAAG ATTCTGAACC ATTTAGACAA 
CCTGTTGATT TGGTTGAATA TCCAGACTAC AGAGATATTA TAGATACCCC AATGGATTTT 
GGAACAGTAA GGGAAACTCT AGATGCGGGA AATTATGACA GCCCTTTGGA GTTTTGCAAA 
GACATCCGGC TGATATTTAG CAATGCAAAA GCGTATACAC CAAACAAAAG ATCAAAGATT 
TATAGTATGA CCTTGAGATT ATCTGCCTTA TTTGAAGAAA AAATGAAGAA AATCTCTTCT 
GATTTTAAAA TTGGTCAAAA ATTCAATGAA AAACTTCGAA GAAGCCAGAG GTTCAAGCAA 
CGGCAAAATT GTAAAGGTGA CAGTCAGCCT AACAAAAGTA TCAGAAACCT CAAGCCGAAG 
AGGTTAAAAT CTCAGACAAA AATAATTCCT GAGTTGGTAG GTTCTCCTAC CCAGTCTACC 
TCAAGTAGGA CAGCTTATCT TGGAACCCAC AAGACAAGTG CTGGTATCTC TTCAGGTGTT 
ACTTCTGGTG ACTCTTCAGA TTCAGCAGAA TCATCAGAAA GGAGGAAAAG AAATAGACCT 
ATAACAAATG GTTCTACATT ATCTGAAAGT GAAGTGGAAG ATTCTTTAGC TACCTCTTTG 
TCATCGTCAG CTTCCAGTAG TTCTGAGGAA AGCAAAGAGA GTTCCAGAGC TCGTGAATCC 
TCCTCACGCA GTGGGCTATC CAGAAGCAGC AATCTCAGGG TAACCAGAAC TAGAGCTGCT 
CAAAGAAAAA CTGGTCCCGT TTCATTAGCA AATGGATGTG GCAGAAAAGC CACTCGAAAG 
AGAGTCTATT TAAGTGATTC TGATAACAAT TCATTGGAGA CTGGTGAAAT TCTAAAAGCC 
AGAGCTGGAA ATAACCGAAA AGTCTTAAGG AAGTGTGCTG CTGTGGCTGC CAATAAAATA 
AAGCTAATGA GTGATGTAGA AGAGAATTCT AGCTCTGAAA GTGTCTGTTC TGGTCGGAAG 
CTGCCTCACC GCAATGCTTC TGCTGTAGCT AGAAAAAAGT TATTACATAA TTCTGAAGAT 
GAACAGAGCT TAAAGTCAGA AATTGAAGAA GAGGAGCTAA AAGATGAAAA TCAACTATTA 
CCAGTGTCCA GTTCTCACAC TGCCCAGAGC AATGTTGATG AATCTGAAAA CAGAGACTCA 
GAGTCAGAAA GTGATTTGCG GGTAGCCCGG AAAAATTGGC ATGCTAATGG TTACAAGTCC 
CATACTCCAG CACCTTCAAA GACAAAATTT CTTAAAATAG AGTCTTCTGA GGAAGACTCT 
AAAAGTCATG ATTCAGATCA TGCATGTAAC AGAACTGCTG GCCCATCAAC GTCTGTGCAG 
AAACTTAAGG CAGAGAGCAT CTCAGAGGAA GCAGATTCTG AACCAGGAAG ATCTGGTGGT 
AGGAAATACA ATACATTTCA CAAGAATGCG AGTTTCTTTA AAAAAACCAA GATTCTGAGT 
GACTCAGAAG ACTCTGAATC TGAAGAGCAA GATAGAGAAG ATGGGAAATG TCATAAAATG 
GAAATGAACC CAATTTCAGG AAATCTGAAC TGTGACCCTA TTGCTATGTC CCAGTGTTCC 
TCAGATCATG GATGTGAAAC TGATTTAGAT TCAGATGATG ACAAAATAGA AAAACCAAAC 
AATTTTATGA AAGATTCTGC ATCACAAGAC AATGGACTAA GCAGAAAAAT TTCCAGGAAA 
AGGGTCTGTT CCAGTGACTC AGACAGTAGT TTACAGGTGG TTAAGAAATC ATCAAAAGCC 
AGAACAGGTC TCCTGAGGAT TACTCGAAGA TGTGCAGCTA CGGCTGCCAA TAAGATCAAG 
CTCATGAGTG ATGTAGAAGA TGTCAGTTTA GAAAATGTGC ACACTAGAAG CAAAAATGGA 
AGGAAAAAAC CTCTCCATCT TGCTTGTACT ACAGCTAAGA AGAAATTGAG TGATTGTGAA 
GGAAGTGTAC ATTGTGAAGT ACCAAGTGAA CAGTATGCCT GTGAAGGCAA GCCACCTGAT 
CCTGACTCCG AAGGTAGTAC AAAAGTGCTT AGTCAGGCTC TAAATGGAGA CTCAGACTCT 
GAAGATATGT TGAATTCAGA ACACAAGCAC AGGCATACCA ATATTCACAA AATAGATGCA 
CCTTCTAAAA GAAAAAGTTC CTCTGTTACA TCTTCAGGAG AAGATTCAAA AAGTCATATT 
CCAGGGAGTG AGACTGATAG GACATTTTCT TCAGAGTCAA CCTTGGCACA AAAAGCTACT 
GCAGAGAATA ATTTTGAAGT GGAACTGAAT TATGGGCTGC GCAGGTGGAA TGGCAGAAGA 
CTCAGGACCT ATGGAAAGGC TCCTTTTAGT AAGACAAAAG TGATTCATGA TTCACAGGAA 
ACAGCAGAGA AGGAAGTAAA AAGGAAGAGA TCGCATCCTG AATTGGAAAA TGTGAAAATC 
TCTGAAACAA CTGGGAATTC AAAGTTTAGA CCTGATACTA GTTCCAAATC ATCAGATTTG 
GGATCTGTAA CTGAATCAGA TATTGACTGT ACTGATAATA CAAAAACCAA AAGGAGGAAA 
ACGAAAGGAA AAGCAAAAGT AGTTAGAAAA GGTAAAACTT TTACAGCTAA CATATCTAAA 
ACTGTGAGAC GTCAAAGACA AAGCAAACGC CCTAGGTTAA GTGTGGATGA TAATGACTGG 
GAGGATTTGG ACTATGCAAA ATCTAAAAGA GTTCTTCGAC GTTCAAAAAT AAAAACGAGA 
AATCAGGGTA GAAGGACTGT GAGATACCAT GATGGGGATG ATGACAGAAG TTTAGAAAAT 
GTGTTAGATT TCAATGGTTG CACCTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_387505.1
CDS	296..7105
Translation	MAEPSSARRPVPLIESELYFLIARYLSAGPCRRAAQVLVQELEQYQLLPKRLDWEGNEHNRSYEELVLSNKHVAPDHLLQICQRIGPMLDKEIPPSISRVTSLLGAGRQSLLRTAKDCRHTVWKGSAFAALHRGRPPEMPVNYGSPPNLVEIHRGKQLTGCSTFSTAFPGTMYQHIKMHRRILGHLSAVYCVAFDRTGHRIFTGSDDCLVKIWSTHNGRLLSTLRGHSAEISDMAVNYENTMIAAGSCDKIIRVWCLRTCAPVAVLQGHTGSITSLQFSPMAKGSQRYMVSTGADGTVCFWQWDLESLKFSPRPLKFTEKPRPGVQMLCSSFSVGGMFLATGSTDHVIRMYFLGFEAPEKIAELESHTDKVDSIQFCNNGDRFLSGSRDGTARIWRFEQLEWRSILLDMATRISGDLSSEEERFMKPKVTMIAWNQNDSIVVTAVNDHVLKVWNSYTGQLLHNLMGHADEVFVLETHPFDSRIMLSAGHDGSIFIWDITKGTKMKHYFNMIEGQGHGAVFDCKFSQDGQHFACTDSHGHLLIFGFGCSKPYEKIPDQMFFHTDYRPLIRDSNNYVLDEQTQQAPHLMPPPFLVDVDGNPHPTKYQRLVPGRENSADEHLIPQLGYVATSDGEVIEQIISLQTNDNDERSPESSILDGMIRQLQQQQDQRMGADQDTIPRGLSNGEETPRRGFRRLSLDIQSPPNIGLRRSGQVEGVRQMHQNAPRSQIATERDLQAWKRRVVVPEVPLGIFRKLEDFRLEKGEEERNLYIIGRKRKTLQLSHKSDSVVLVSQSRQRTCRRKYPNYGRRNRSWRELSSGNESSSSVRHETSCDQSEGSGSSEEDEWRSDRKSESYSESSSDSSSRYSDWTADAGINLQPPLRTSCRRRITRFCSSSEDEISTENLSPPKRRRKRKKENKPKKENLRRMTPAELANMEHLYEFHPPVWITDTTLRKSPFVPQMGDEVIYFRQGHEAYIEAVRRNNIYELNPNKEPWRKMDLRDQELVKIVGIRYEVGPPTLCCLKLAFIDPATGKLMDKSFSIRYHDMPDVIDFLVLRQFYDEARQRNWQSCDRFRSIIDDAWWFGTVLSQEPYQPQYPDSHFQCYIVRWDNTEIEKLSPWDMEPIPDNVDPPEELGASISVTTDELEKLLYKPQAGEWGQKSRDEECDRIISGIDQLLNLDIAAAFAGPVDLCTYPKYCTVVAYPTDLYTIRMRLVNRFYRRLSALVWEVRYIEHNARTFNEPESVIARSAKKITDQLLKFIKNQHCTNISELSNTSENDEQNAEDLDDSDLPKTSSGRRRVHDGKKSIRATNYVESNWKKQCKELVNLIFQCEDSEPFRQPVDLVEYPDYRDIIDTPMDFGTVRETLDAGNYDSPLEFCKDIRLIFSNAKAYTPNKRSKIYSMTLRLSALFEEKMKKISSDFKIGQKFNEKLRRSQRFKQRQNCKGDSQPNKSIRNLKPKRLKSQTKIIPELVGSPTQSTSSRTAYLGTHKTSAGISSGVTSGDSSDSAESSERRKRNRPITNGSTLSESEVEDSLATSLSSSASSSSEESKESSRARESSSRSGLSRSSNLRVTRTRAAQRKTGPVSLANGCGRKATRKRVYLSDSDNNSLETGEILKARAGNNRKVLRKCAAVAANKIKLMSDVEENSSSESVCSGRKLPHRNASAVARKKLLHNSEDEQSLKSEIEEEELKDENQLLPVSSSHTAQSNVDESENRDSESESDLRVARKNWHANGYKSHTPAPSKTKFLKIESSEEDSKSHDSDHACNRTAGPSTSVQKLKAESISEEADSEPGRSGGRKYNTFHKNASFFKKTKILSDSEDSESEEQDREDGKCHKMEMNPISGNLNCDPIAMSQCSSDHGCETDLDSDDDKIEKPNNFMKDSASQDNGLSRKISRKRVCSSDSDSSLQVVKKSSKARTGLLRITRRCAATAANKIKLMSDVEDVSLENVHTRSKNGRKKPLHLACTTAKKKLSDCEGSVHCEVPSEQYACEGKPPDPDSEGSTKVLSQALNGDSDSEDMLNSEHKHRHTNIHKIDAPSKRKSSSVTSSGEDSKSHIPGSETDRTFSSESTLAQKATAENNFEVELNYGLRRWNGRRLRTYGKAPFSKTKVIHDSQETAEKEVKRKRSHPELENVKISETTGNSKFRPDTSSKSSDLGSVTESDIDCTDNTKTKRRKTKGKAKVVRKGKTFTANISKTVRRQRQSKRPRLSVDDNDWEDLDYAKSKRVLRRSKIKTRNQGRRTVRYHDGDDDRSLENVLDFNGCTL

Target ORF information:

RefSeq Version	NM_033656.3
Organism	Homo sapiens(human)
Definition	Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033656.3

ORF Insert Sequence:

ATGGCGGAGC CGTCGTCCGC CCGACGCCCG GTGCCTCTCA TCGAGTCGGA GCTGTACTTC 
CTTATCGCCC GGTACCTATC GGCGGGCCCG TGTCGGAGAG CGGCCCAGGT GCTGGTGCAG 
GAGCTGGAGC AGTACCAGTT GTTGCCGAAG AGATTGGACT GGGAGGGCAA CGAGCACAAC 
AGGAGCTACG AGGAGTTGGT CTTGTCCAAT AAGCATGTGG CTCCTGATCA TCTTTTGCAA 
ATCTGCCAGC GCATCGGTCC TATGTTGGAT AAAGAAATTC CACCCAGTAT TTCAAGAGTC 
ACTTCTTTAC TTGGTGCAGG AAGGCAGTCT TTGCTACGTA CAGCAAAAGA CTGCAGGCAC 
ACAGTTTGGA AGGGCTCTGC CTTTGCTGCT CTTCATAGAG GAAGACCTCC TGAAATGCCA 
GTGAATTATG GTTCCCCACC AAATCTTGTG GAGATACATC GAGGAAAACA ACTCACAGGG 
TGTTCCACTT TTAGTACAGC ATTTCCAGGA ACTATGTATC AGCATATAAA AATGCACAGA 
AGGATTCTCG GACATCTATC TGCTGTTTAC TGTGTAGCAT TTGATAGGAC AGGACATAGA 
ATCTTTACAG GTTCAGATGA CTGTTTGGTA AAGATTTGGT CAACACATAA TGGCCGCTTG 
TTATCTACAT TAAGAGGTCA TTCTGCAGAA ATTTCAGATA TGGCAGTAAA CTATGAGAAT 
ACAATGATTG CTGCGGGGAG CTGTGATAAA ATTATTAGAG TGTGGTGCTT GAGAACTTGT 
GCCCCAGTTG CTGTGCTCCA AGGACACACA GGATCAATTA CATCTTTACA GTTTAGCCCG 
ATGGCCAAAG GCTCTCAAAG ATACATGGTT TCCACTGGTG CTGATGGGAC AGTTTGCTTT 
TGGCAATGGG ATTTAGAATC CTTAAAATTT AGCCCACGTC CCCTGAAGTT CACTGAAAAG 
CCTAGGCCAG GCGTTCAAAT GCTTTGTTCT TCTTTTAGTG TTGGTGGTAT GTTTTTAGCC 
ACAGGTAGTA CTGATCATGT AATCAGAATG TATTTTTTGG GTTTTGAAGC ACCCGAAAAA 
ATCGCAGAAC TTGAAAGCCA CACTGATAAA GTAGATAGTA TCCAATTTTG TAACAATGGT 
GATCGGTTCC TAAGTGGTAG CAGAGATGGA ACAGCACGGA TTTGGAGATT TGAGCAGTTA 
GAATGGAGGA GCATTTTATT GGATATGGCT ACCAGAATCT CAGGGGACTT ATCTTCCGAA 
GAGGAAAGGT TTATGAAACC TAAAGTAACA ATGATAGCTT GGAATCAAAA TGATAGCATT 
GTTGTCACAG CTGTGAATGA TCATGTCCTC AAAGTGTGGA ATTCTTACAC TGGACAACTG 
CTTCATAACT TAATGGGACA TGCTGATGAA GTATTTGTTC TGGAGACACA TCCCTTTGAT 
TCCAGAATTA TGTTATCTGC AGGACATGAT GGCAGCATAT TTATATGGGA TATTACAAAA 
GGTACCAAGA TGAAACATTA TTTTAATATG ATTGAAGGAC AAGGACATGG AGCTGTGTTT 
GACTGTAAGT TTTCACAGGA TGGACAGCAT TTTGCCTGTA CAGATTCTCA TGGGCACCTT 
CTGATATTTG GTTTTGGATG CAGCAAACCA TATGAAAAGA TTCCTGATCA GATGTTCTTC 
CATACTGACT ATCGACCACT TATTAGAGAT TCTAATAATT ATGTCTTAGA TGAGCAAACT 
CAGCAGGCTC CTCATCTTAT GCCTCCACCA TTCTTGGTAG ATGTAGATGG AAATCCTCAT 
CCAACCAAGT ATCAGAGATT AGTACCAGGC CGAGAAAATT CTGCAGATGA ACATTTGATT 
CCACAGCTGG GCTATGTGGC AACAAGTGAT GGAGAGGTGA TTGAACAAAT TATAAGCCTG 
CAAACCAATG ATAATGATGA ACGCAGCCCA GAATCGAGTA TTCTTGATGG AATGATAAGA 
CAGTTGCAGC AGCAGCAAGA TCAGAGAATG GGAGCAGATC AGGATACTAT TCCAAGAGGA 
CTTTCAAATG GTGAAGAAAC ACCCCGGAGA GGTTTTAGAA GGCTGAGCTT AGACATTCAG 
TCCCCTCCAA ATATTGGTCT GCGTCGTAGT GGACAAGTTG AAGGTGTTCG TCAGATGCAT 
CAAAACGCTC CACGCAGTCA GATTGCTACA GAACGTGACC TGCAGGCTTG GAAACGAAGA 
GTGGTTGTAC CAGAGGTACC ACTAGGCATA TTTAGGAAGC TGGAAGACTT CCGATTAGAG 
AAAGGTGAAG AGGAAAGAAA TCTTTATATA ATAGGAAGAA AAAGAAAGAC TCTTCAGCTC 
TCACATAAGT CGGATTCAGT GGTTTTGGTA TCACAGTCTA GACAAAGGAC ATGTAGGCGT 
AAATATCCAA ATTATGGTAG AAGAAATCGT AGCTGGCGTG AGTTATCTTC TGGAAATGAG 
TCTTCAAGCT CTGTAAGACA TGAGACTTCC TGTGATCAGA GTGAAGGTTC TGGTTCTTCA 
GAAGAGGATG AATGGAGAAG TGACAGAAAA AGTGAGAGTT ACAGCGAAAG TTCAAGTGAC 
TCTTCATCTA GATATTCCGA TTGGACAGCT GATGCGGGCA TCAATTTGCA GCCTCCTTTA 
AGAACATCAT GTCGTCGACG AATTACTCGA TTTTGTAGTA GTTCAGAAGA TGAAATATCT 
ACTGAGAATT TATCTCCTCC AAAAAGAAGA CGAAAGAGAA AGAAAGAAAA TAAGCCTAAG 
AAGGAGAATT TGCGGAGGAT GACTCCAGCA GAGCTTGCAA ATATGGAGCA TTTATATGAA 
TTTCACCCTC CAGTTTGGAT TACTGACACC ACACTTAGAA AATCTCCTTT TGTTCCTCAA 
ATGGGTGATG AGGTAATATA TTTTCGACAG GGTCATGAAG CTTATATTGA GGCTGTAAGA 
AGAAATAATA TTTATGAACT GAACCCTAAT AAGGAGCCAT GGAGAAAAAT GGATCTTAGG 
GATCAAGAAT TGGTTAAAAT AGTTGGAATA CGATATGAAG TTGGGCCCCC TACACTCTGT 
TGCCTAAAAC TAGCATTTAT AGATCCAGCA ACTGGAAAAC TTATGGACAA ATCTTTCTCT 
ATTAGATATC ATGATATGCC AGATGTTATT GACTTTCTTG TATTGCGTCA ATTTTATGAT 
GAAGCAAGAC AGAGGAATTG GCAGTCTTGT GACAGATTCC GCTCTATTAT TGATGATGCT 
TGGTGGTTTG GAACAGTGTT AAGTCAAGAG CCATATCAAC CACAGTATCC TGATAGTCAT 
TTCCAGTGTT ATATTGTTAG GTGGGATAAT ACTGAAATTG AAAAACTTAG CCCATGGGAC 
ATGGAACCAA TTCCTGATAA TGTTGATCCA CCTGAAGAAT TAGGAGCTAG TATTTCTGTC 
ACAACAGATG AGCTAGAGAA ATTGCTCTAT AAACCACAAG CTGGTGAATG GGGTCAGAAA 
TCAAGAGATG AAGAATGTGA TAGAATTATC AGTGGTATAG ATCAACTTTT GAATCTTGAT 
ATAGCAGCAG CTTTTGCAGG CCCTGTTGAT TTGTGTACAT ACCCGAAGTA CTGTACTGTA 
GTAGCTTATC CAACCGATCT TTACACAATT CGAATGAGAC TTGTTAATCG ATTTTACAGG 
AGGCTGTCTG CGTTAGTTTG GGAAGTCAGA TATATAGAAC ATAATGCCAG AACATTTAAC 
GAACCTGAGA GTGTAATTGC AAGATCAGCT AAAAAGATAA CTGACCAACT TTTAAAATTT 
ATCAAGAATC AACACTGTAC AAATATCTCA GAACTTTCTA ACACATCTGA AAATGATGAG 
CAAAATGCTG AGGATTTGGA TGATAGTGAT CTTCCTAAAA CATCTTCTGG AAGGAGGAGA 
GTCCATGATG GGAAAAAAAG CATCAGAGCT ACGAACTATG TTGAAAGCAA CTGGAAGAAA 
CAGTGTAAGG AACTAGTGAA CTTAATTTTT CAGTGTGAAG ATTCTGAACC ATTTAGACAA 
CCTGTTGATT TGGTTGAATA TCCAGACTAC AGAGATATTA TAGATACCCC AATGGATTTT 
GGAACAGTAA GGGAAACTCT AGATGCGGGA AATTATGACA GCCCTTTGGA GTTTTGCAAA 
GACATCCGGC TGATATTTAG CAATGCAAAA GCGTATACAC CAAACAAAAG ATCAAAGATT 
TATAGTATGA CCTTGAGATT ATCTGCCTTA TTTGAAGAAA AAATGAAGAA AATCTCTTCT 
GATTTTAAAA TTGGTCAAAA ATTCAATGAA AAACTTCGAA GAAGCCAGAG GTTCAAGCAA 
CGGCAAAATT GTAAAGGTGA CAGTCAGCCT AACAAAAGTA TCAGAAACCT CAAGCCGAAG 
AGGTTAAAAT CTCAGACAAA AATAATTCCT GAGTTGGTAG GTTCTCCTAC CCAGTCTACC 
TCAAGTAGGA CAGCTTATCT TGGAACCCAC AAGACAAGTG CTGGTATCTC TTCAGGTGTT 
ACTTCTGGTG ACTCTTCAGA TTCAGCAGAA TCATCAGAAA GGAGGAAAAG AAATAGACCT 
ATAACAAATG GTTCTACATT ATCTGAAAGT GAAGTGGAAG ATTCTTTAGC TACCTCTTTG 
TCATCGTCAG CTTCCAGTAG TTCTGAGGAA AGCAAAGAGA GTTCCAGAGC TCGTGAATCC 
TCCTCACGCA GTGGGCTATC CAGAAGCAGC AATCTCAGGG TAACCAGAAC TAGAGCTGCT 
CAAAGAAAAA CTGGTCCCGT TTCATTAGCA AATGGATGTG GCAGAAAAGC CACTCGAAAG 
AGAGTCTATT TAAGTGATTC TGATAACAAT TCATTGGAGA CTGGTGAAAT TCTAAAAGCC 
AGAGCTGGAA ATAACCGAAA AGTCTTAAGG AAGTGTGCTG CTGTGGCTGC CAATAAAATA 
AAGCTAATGA GTGATGTAGA AGAGAATTCT AGCTCTGAAA GTGTCTGTTC TGGTCGGAAG 
CTGCCTCACC GCAATGCTTC TGCTGTAGCT AGAAAAAAGT TATTACATAA TTCTGAAGAT 
GAACAGAGCT TAAAGTCAGA AATTGAAGAA GAGGAGCTAA AAGATGAAAA TCAACTATTA 
CCAGTGTCCA GTTCTCACAC TGCCCAGAGC AATGTTGATG AATCTGAAAA CAGAGACTCA 
GAGTCAGAAA GTGATTTGCG GGTAGCCCGG AAAAATTGGC ATGCTAATGG TTACAAGTCC 
CATACTCCAG CACCTTCAAA GACAAAATTT CTTAAAATAG AGTCTTCTGA GGAAGACTCT 
AAAAGTCATG ATTCAGATCA TGCATGTAAC AGAACTGCTG GCCCATCAAC GTCTGTGCAG 
AAACTTAAGG CAGAGAGCAT CTCAGAGGAA GCAGATTCTG AACCAGGAAG ATCTGGTGGT 
AGGAAATACA ATACATTTCA CAAGAATGCG AGTTTCTTTA AAAAAACCAA GATTCTGAGT 
GACTCAGAAG ACTCTGAATC TGAAGAGCAA GATAGAGAAG ATGGGAAATG TCATAAAATG 
GAAATGAACC CAATTTCAGG AAATCTGAAC TGTGACCCTA TTGCTATGTC CCAGTGTTCC 
TCAGATCATG GATGTGAAAC TGATTTAGAT TCAGATGATG ACAAAATAGA AAAACCAAAC 
AATTTTATGA AAGATTCTGC ATCACAAGAC AATGGACTAA GCAGAAAAAT TTCCAGGAAA 
AGGGTCTGTT CCAGTGACTC AGACAGTAGT TTACAGGTGG TTAAGAAATC ATCAAAAGCC 
AGAACAGGTC TCCTGAGGAT TACTCGAAGA TGTGCAGCTA CGGCTGCCAA TAAGATCAAG 
CTCATGAGTG ATGTAGAAGA TGTCAGTTTA GAAAATGTGC ACACTAGAAG CAAAAATGGA 
AGGAAAAAAC CTCTCCATCT TGCTTGTACT ACAGCTAAGA AGAAATTGAG TGATTGTGAA 
GGAAGTGTAC ATTGTGAAGT ACCAAGTGAA CAGTATGCCT GTGAAGGCAA GCCACCTGAT 
CCTGACTCCG AAGGTAGTAC AAAAGTGCTT AGTCAGGCTC TAAATGGAGA CTCAGACTCT 
GAAGATATGT TGAATTCAGA ACACAAGCAC AGGCATACCA ATATTCACAA AATAGATGCA 
CCTTCTAAAA GAAAAAGTTC CTCTGTTACA TCTTCAGGAG AAGATTCAAA AAGTCATATT 
CCAGGGAGTG AGACTGATAG GACATTTTCT TCAGAGTCAA CCTTGGCACA AAAAGCTACT 
GCAGAGAATA ATTTTGAAGT GGAACTGAAT TATGGGCTGC GCAGGTGGAA TGGCAGAAGA 
CTCAGGACCT ATGGAAAGGC TCCTTTTAGT AAGACAAAAG TGATTCATGA TTCACAGGAA 
ACAGCAGAGA AGGAAGTAAA AAGGAAGAGA TCGCATCCTG AATTGGAAAA TGTGAAAATC 
TCTGAAACAA CTGGGAATTC AAAGTTTAGA CCTGATACTA GTTCCAAATC ATCAGATTTG 
GGATCTGTAA CTGAATCAGA TATTGACTGT ACTGATAATA CAAAAACCAA AAGGAGGAAA 
ACGAAAGGAA AAGCAAAAGT AGTTAGAAAA GGTAAAACTT TTACAGCTAA CATATCTAAA 
ACTGTGAGAC GTCAAAGACA AAGCAAACGC CCTAGGTTAA GTGTGGATGA TAATGACTGG 
GAGGATTTGG ACTATGCAAA ATCTAAAAGA GTTCTTCGAC GTTCAAAAAT AAAAACGAGA 
AATCAGGGTA GAAGGACTGT GAGATACCAT GATGGGGATG ATGACAGAAG TTTAGAAAAT 
GTGTTAGATT TCAATGGTTG CACCTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRWD1 ( XM_011529611.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527913.1 Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant X1, mRNA.

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CloneID	OHu68247
Clone ID Related Accession (Same CDS sequence)	XM_011529611.1
Accession Version	XM_011529611.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bromodomain and WD repeat-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGC CGTCGTCCGC CCGACGCCCG GTGCCTCTCA TCGAGTCGGA GCTGTACTTC 
CTTATCGCCC GGTACCTATC GGCGGGCCCG TGTCGGAGAG CGGCCCAGGT GCTGGTGCAG 
GAGCTGGAGC AGTACCAGTT GTTGCCGAAG AGATTGGACT GGGAGGGCAA CGAGCACAAC 
AGGAGCTACG AGGAGTTGGT CTTGTCCAAT AAGCATGTGG CTCCTGATCA TCTTTTGCAA 
ATCTGCCAGC GCATCGGTCC TATGTTGGAT AAAGAAATTC CACCCAGTAT TTCAAGAGTC 
ACTTCTTTAC TTGGTGCAGG AAGGCAGTCT TTGCTACGTA CAGCAAAAGA CTGCAGGCAC 
ACAGTTTGGA AGGGCTCTGC CTTTGCTGCT CTTCATAGAG GAAGACCTCC TGAAATGCCA 
GTGAATTATG GTTCCCCACC AAATCTTGTG GAGATACATC GAGGAAAACA ACTCACAGGG 
TGTTCCACTT TTAGTACAGC ATTTCCAGGA ACTATGTATC AGCATATAAA AATGCACAGA 
AGGATTCTCG GACATCTATC TGCTGTTTAC TGTGTAGCAT TTGATAGGAC AGGACATAGA 
ATCTTTACAG GTTCAGATGA CTGTTTGGTA AAGATTTGGT CAACACATAA TGGCCGCTTG 
TTATCTACAT TAAGAGGTCA TTCTGCAGAA ATTTCAGATA TGGCAGTAAA CTATGAGAAT 
ACAATGATTG CTGCGGGGAG CTGTGATAAA ATTATTAGAG TGTGGTGCTT GAGAACTTGT 
GCCCCAGTTG CTGTGCTCCA AGGACACACA GGATCAATTA CATCTTTACA GTTTAGCCCG 
ATGGCCAAAG GCTCTCAAAG ATACATGGTT TCCACTGGTG CTGATGGGAC AGTTTGCTTT 
TGGCAATGGG ATTTAGAATC CTTAAAATTT AGCCCACGTC CCCTGAAGTT CACTGAAAAG 
CCTAGGCCAG GCGTTCAAAT GCTTTGTTCT TCTTTTAGTG TTGGTGGTAT GTTTTTAGCC 
ACAGGTAGTA CTGATCATGT AATCAGAATG TATTTTTTGG GTTTTGAAGC ACCCGAAAAA 
ATCGCAGAAC TTGAAAGCCA CACTGATAAA GTAGATAGTA TCCAATTTTG TAACAATGGT 
GATCGGTTCC TAAGTGGTAG CAGAGATGGA ACAGCACGGA TTTGGAGATT TGAGCAGTTA 
GAATGGAGGA GCATTTTATT GGATATGGCT ACCAGAATCT CAGGGGACTT ATCTTCCGAA 
GAGGAAAGGT TTATGAAACC TAAAGTAACA ATGATAGCTT GGAATCAAAA TGATAGCATT 
GTTGTCACAG CTGTGAATGA TCATGTCCTC AAAGTGTGGA ATTCTTACAC TGGACAACTG 
CTTCATAACT TAATGGGACA TGCTGATGAA GTATTTGTTC TGGAGACACA TCCCTTTGAT 
TCCAGAATTA TGTTATCTGC AGGACATGAT GGCAGCATAT TTATATGGGA TATTACAAAA 
GGTACCAAGA TGAAACATTA TTTTAATATG ATTGAAGGAC AAGGACATGG AGCTGTGTTT 
GACTGTAAGT TTTCACAGGA TGGACAGCAT TTTGCCTGTA CAGATTCTCA TGGGCACCTT 
CTGATATTTG GTTTTGGATG CAGCAAACCA TATGAAAAGA TTCCTGATCA GATGTTCTTC 
CATACTGACT ATCGACCACT TATTAGAGAT TCTAATAATT ATGTCTTAGA TGAGCAAACT 
CAGCAGGCTC CTCATCTTAT GCCTCCACCA TTCTTGGTAG ATGTAGATGG AAATCCTCAT 
CCAACCAAGT ATCAGAGATT AGTACCAGGC CGAGAAAATT CTGCAGATGA ACATTTGATT 
CCACAGCTGG GCTATGTGGC AACAAGTGAT GGAGAGGTGA TTGAACAAAT TATAAGCCTG 
CAAACCAATG ATAATGATGA ACGCAGCCCA GAATCGAGTA TTCTTGATGG AATGATAAGA 
CAGTTGCAGC AGCAGCAAGA TCAGAGAATG GGAGCAGATC AGGATACTAT TCCAAGAGGA 
CTTTCAAATG GTGAAGAAAC ACCCCGGAGA GGTTTTAGAA GGCTGAGCTT AGACATTCAG 
TCCCCTCCAA ATATTGGTCT GCGTCGTAGT GGACAAGTTG AAGGTGTTCG TCAGATGCAT 
CAAAACGCTC CACGCAGTCA GATTGCTACA GAACGTGACC TGCAGGCTTG GAAACGAAGA 
GTGGTTGTAC CAGAGGTACC ACTAGGCATA TTTAGGAAGC TGGAAGACTT CCGATTAGAG 
AAAGGTGAAG AGGAAAGAAA TCTTTATATA ATAGGAAGAA AAAGAAAGAC TCTTCAGCTC 
TCACATAAGT CGGATTCAGT GGTTTTGGTA TCACAGTCTA GACAAAGGAC ATGTAGGCGT 
AAATATCCAA ATTATGGTAG AAGAAATCGT AGCTGGCGTG AGTTATCTTC TGGAAATGAG 
TCTTCAAGCT CTGTAAGACA TGAGACTTCC TGTGATCAGA GTGAAGGTTC TGGTTCTTCA 
GAAGAGGATG AATGGAGAAG TGACAGAAAA AGTGAGAGTT ACAGCGAAAG TTCAAGTGAC 
TCTTCATCTA GATATTCCGA TTGGACAGCT GATGCGGGCA TCAATTTGCA GCCTCCTTTA 
AGAACATCAT GTCGTCGACG AATTACTCGA TTTTGTAGTA GTTCAGAAGA TGAAATATCT 
ACTGAGAATT TATCTCCTCC AAAAAGAAGA CGAAAGAGAA AGAAAGAAAA TAAGCCTAAG 
AAGGAGAATT TGCGGAGGAT GACTCCAGCA GAGCTTGCAA ATATGGAGCA TTTATATGAA 
TTTCACCCTC CAGTTTGGAT TACTGACACC ACACTTAGAA AATCTCCTTT TGTTCCTCAA 
ATGGGTGATG AGGTAATATA TTTTCGACAG GGTCATGAAG CTTATATTGA GGCTGTAAGA 
AGAAATAATA TTTATGAACT GAACCCTAAT AAGGAGCCAT GGAGAAAAAT GGATCTTAGG 
GATCAAGAAT TGGTTAAAAT AGTTGGAATA CGATATGAAG TTGGGCCCCC TACACTCTGT 
TGCCTAAAAC TAGCATTTAT AGATCCAGCA ACTGGAAAAC TTATGGACAA ATCTTTCTCT 
ATTAGATATC ATGATATGCC AGATGTTATT GACTTTCTTG TATTGCGTCA ATTTTATGAT 
GAAGCAAGAC AGAGGAATTG GCAGTCTTGT GACAGATTCC GCTCTATTAT TGATGATGCT 
TGGTGGTTTG GAACAGTGTT AAGTCAAGAG CCATATCAAC CACAGTATCC TGATAGTCAT 
TTCCAGTGTT ATATTGTTAG GTGGGATAAT ACTGAAATTG AAAAACTTAG CCCATGGGAC 
ATGGAACCAA TTCCTGATAA TGTTGATCCA CCTGAAGAAT TAGGAGCTAG TATTTCTGTC 
ACAACAGATG AGCTAGAGAA ATTGCTCTAT AAACCACAAG CTGGTGAATG GGGTCAGAAA 
TCAAGAGATG AAGAATGTGA TAGAATTATC AGTGGTATAG ATCAACTTTT GAATCTTGAT 
ATAGCAGCAG CTTTTGCAGG CCCTGTTGAT TTGTGTACAT ACCCGAAGTA CTGTACTGTA 
GTAGCTTATC CAACCGATCT TTACACAATT CGAATGAGAC TTGTTAATCG ATTTTACAGG 
AGGCTGTCTG CGTTAGTTTG GGAAGTCAGA TATATAGAAC ATAATGCCAG AACATTTAAC 
GAACCTGAGA GTGTAATTGC AAGATCAGCT AAAAAGATAA CTGACCAACT TTTAAAATTT 
ATCAAGAATC AACACTGTAC AAATATCTCA GAACTTTCTA ACACATCTGA AAATGATGAG 
CAAAATGCTG AGGATTTGGA TGATAGTGAT CTTCCTAAAA CATCTTCTGG AAGGAGGAGA 
GACTACAGAG ATATTATAGA TACCCCAATG GATTTTGGAA CAGTAAGGGA AACTCTAGAT 
GCGGGAAATT ATGACAGCCC TTTGGAGTTT TGCAAAGACA TCCGGCTGAT ATTTAGCAAT 
GCAAAAGCGT ATACACCAAA CAAAAGATCA AAGATTTATA GTATGACCTT GAGATTATCT 
GCCTTATTTG AAGAAAAAAT GAAGAAAATC TCTTCTGATT TTAAAATTGG TCAAAAATTC 
AATGAAAAAC TTCGAAGAAG CCAGAGGTTC AAGCAACGGC AAAATTGTAA AGGTGACAGT 
CAGCCTAACA AAAGTATCAG AAACCTCAAG CCGAAGAGGT TAAAATCTCA GACAAAAATA 
ATTCCTGAGT TGGTAGGTTC TCCTACCCAG TCTACCTCAA GTAGGACAGC TTATCTTGGA 
ACCCACAAGA CAAGTGCTGG TATCTCTTCA GGTGTTACTT CTGGTGACTC TTCAGATTCA 
GCAGAATCAT CAGAAAGGAG GAAAAGAAAT AGACCTATAA CAAATGGTTC TACATTATCT 
GAAAGTGAAG TGGAAGATTC TTTAGCTACC TCTTTGTCAT CGTCAGCTTC CAGTAGTTCT 
GAGGAAAGCA AAGAGAGTTC CAGAGCTCGT GAATCCTCCT CACGCAGTGG GCTATCCAGA 
AGCAGCAATC TCAGGGTAAC CAGAACTAGA GCTGCTCAAA GAAAAACTGG TCCCGTTTCA 
TTAGCAAATG GATGTGGCAG AAAAGCCACT CGAAAGAGAG TCTATTTAAG TGATTCTGAT 
AACAATTCAT TGGAGACTGG TGAAATTCTA AAAGCCAGAG CTGGAAATAA CCGAAAAGTC 
TTAAGGAAGT GTGCTGCTGT GGCTGCCAAT AAAATAAAGC TAATGAGTGA TGTAGAAGAG 
AATTCTAGCT CTGAAAGTGT CTGTTCTGGT CGGAAGCTGC CTCACCGCAA TGCTTCTGCT 
GTAGCTAGAA AAAAGTTATT ACATAATTCT GAAGATGAAC AGAGCTTAAA GTCAGAAATT 
GAAGAAGAGG AGCTAAAAGA TGAAAATCAA CTATTACCAG TGTCCAGTTC TCACACTGCC 
CAGAGCAATG TTGATGAATC TGAAAACAGA GACTCAGAGT CAGAAAGTGA TTTGCGGGTA 
GCCCGGAAAA ATTGGCATGC TAATGGTTAC AAGTCCCATA CTCCAGCACC TTCAAAGACA 
AAATTTCTTA AAATAGAGTC TTCTGAGGAA GACTCTAAAA GTCATGATTC AGATCATGCA 
TGTAACAGAA CTGCTGGCCC ATCAACGTCT GTGCAGAAAC TTAAGGCAGA GAGCATCTCA 
GAGGAAGCAG ATTCTGAACC AGGAAGATCT GGTGGTAGGA AATACAATAC ATTTCACAAG 
AATGCGAGTT TCTTTAAAAA AACCAAGATT CTGAGTGACT CAGAAGACTC TGAATCTGAA 
GAGCAAGATA GAGAAGATGG GAAATGTCAT AAAATGGAAA TGAACCCAAT TTCAGGAAAT 
CTGAACTGTG ACCCTATTGC TATGTCCCAG TGTTCCTCAG ATCATGGATG TGAAACTGAT 
TTAGATTCAG ATGATGACAA AATAGAAAAA CCAAACAATT TTATGAAAGA TTCTGCATCA 
CAAGACAATG GACTAAGCAG AAAAATTTCC AGGAAAAGGG TCTGTTCCAG TGACTCAGAC 
AGTAGTTTAC AGGTGGTTAA GAAATCATCA AAAGCCAGAA CAGGTCTCCT GAGGATTACT 
CGAAGATGTG CAGCTACGGC TGCCAATAAG ATCAAGCTCA TGAGTGATGT AGAAGATGTC 
AGTTTAGAAA ATGTGCACAC TAGAAGCAAA AATGGAAGGA AAAAACCTCT CCATCTTGCT 
TGTACTACAG CTAAGAAGAA ATTGAGTGAT TGTGAAGGAA GTGTACATTG TGAAGTACCA 
AGTGAACAGT ATGCCTGTGA AGGCAAGCCA CCTGATCCTG ACTCCGAAGG TAGTACAAAA 
GTGCTTAGTC AGGCTCTAAA TGGAGACTCA GACTCTGAAG ATATGTTGAA TTCAGAACAC 
AAGCACAGGC ATACCAATAT TCACAAAATA GATGCACCTT CTAAAAGAAA AAGTTCCTCT 
GTTACATCTT CAGGAGAAGA TTCAAAAAGT CATATTCCAG GGAGTGAGAC TGATAGGACA 
TTTTCTTCAG AGTCAACCTT GGCACAAAAA GCTACTGCAG AGAATAATTT TGAAGTGGAA 
CTGAATTATG GGCTGCGCAG GTGGAATGGC AGAAGACTCA GGACCTATGG AAAGGCTCCT 
TTTAGTAAGA CAAAAGTGAT TCATGATTCA CAGGAAACAG CAGAGAAGGA AGTAAAAAGG 
AAGAGATCGC ATCCTGAATT GGAAAATGTG AAAATCTCTG AAACAACTGG GAATTCAAAG 
TTTAGACCTG ATACTAGTTC CAAATCATCA GATTTGGGAT CTGTAACTGA ATCAGATATT 
GACTGTACTG ATAATACAAA AACCAAAAGG AGGAAAACGA AAGGAAAAGC AAAAGTAGTT 
AGAAAAGAAT TTGTTCCTAG AGACAGAGAA CCCAATACAA AAGTGAGAAC ATGTATGCAT 
AATCAGAAGG ATGCAGTGCA GATGCCTAGT GAAACTCTGA AAGCAAAAAT GGTACCTGAG 
AAAGTTCCCC GCAGATGTGC TACTGTTGCT GCAAATAAAA TAAAGATAAT GAGTAATCTA 
AAAGAAACGA TCTCAGGACC AGAAAATGTC TGGATTAGGA AGAGTAGCAG AAAACTGCCC 
CATCGAAATG CTTCTGCTGC GGCTAAGAAA AAATTACTGA ATGTTTATAA AGAGGATGAT 
ACAACCATAA ATTCTGAAAG TGAAAAAGAG CTAGAAGATA TTAATAGAAA AATGCTTTTT 
TTAAGGGGGT TCAGATCCTG GAAAGAAAAT GCACAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527913.1
CDS	329..7147
Translation	MAEPSSARRPVPLIESELYFLIARYLSAGPCRRAAQVLVQELEQYQLLPKRLDWEGNEHNRSYEELVLSNKHVAPDHLLQICQRIGPMLDKEIPPSISRVTSLLGAGRQSLLRTAKDCRHTVWKGSAFAALHRGRPPEMPVNYGSPPNLVEIHRGKQLTGCSTFSTAFPGTMYQHIKMHRRILGHLSAVYCVAFDRTGHRIFTGSDDCLVKIWSTHNGRLLSTLRGHSAEISDMAVNYENTMIAAGSCDKIIRVWCLRTCAPVAVLQGHTGSITSLQFSPMAKGSQRYMVSTGADGTVCFWQWDLESLKFSPRPLKFTEKPRPGVQMLCSSFSVGGMFLATGSTDHVIRMYFLGFEAPEKIAELESHTDKVDSIQFCNNGDRFLSGSRDGTARIWRFEQLEWRSILLDMATRISGDLSSEEERFMKPKVTMIAWNQNDSIVVTAVNDHVLKVWNSYTGQLLHNLMGHADEVFVLETHPFDSRIMLSAGHDGSIFIWDITKGTKMKHYFNMIEGQGHGAVFDCKFSQDGQHFACTDSHGHLLIFGFGCSKPYEKIPDQMFFHTDYRPLIRDSNNYVLDEQTQQAPHLMPPPFLVDVDGNPHPTKYQRLVPGRENSADEHLIPQLGYVATSDGEVIEQIISLQTNDNDERSPESSILDGMIRQLQQQQDQRMGADQDTIPRGLSNGEETPRRGFRRLSLDIQSPPNIGLRRSGQVEGVRQMHQNAPRSQIATERDLQAWKRRVVVPEVPLGIFRKLEDFRLEKGEEERNLYIIGRKRKTLQLSHKSDSVVLVSQSRQRTCRRKYPNYGRRNRSWRELSSGNESSSSVRHETSCDQSEGSGSSEEDEWRSDRKSESYSESSSDSSSRYSDWTADAGINLQPPLRTSCRRRITRFCSSSEDEISTENLSPPKRRRKRKKENKPKKENLRRMTPAELANMEHLYEFHPPVWITDTTLRKSPFVPQMGDEVIYFRQGHEAYIEAVRRNNIYELNPNKEPWRKMDLRDQELVKIVGIRYEVGPPTLCCLKLAFIDPATGKLMDKSFSIRYHDMPDVIDFLVLRQFYDEARQRNWQSCDRFRSIIDDAWWFGTVLSQEPYQPQYPDSHFQCYIVRWDNTEIEKLSPWDMEPIPDNVDPPEELGASISVTTDELEKLLYKPQAGEWGQKSRDEECDRIISGIDQLLNLDIAAAFAGPVDLCTYPKYCTVVAYPTDLYTIRMRLVNRFYRRLSALVWEVRYIEHNARTFNEPESVIARSAKKITDQLLKFIKNQHCTNISELSNTSENDEQNAEDLDDSDLPKTSSGRRRDYRDIIDTPMDFGTVRETLDAGNYDSPLEFCKDIRLIFSNAKAYTPNKRSKIYSMTLRLSALFEEKMKKISSDFKIGQKFNEKLRRSQRFKQRQNCKGDSQPNKSIRNLKPKRLKSQTKIIPELVGSPTQSTSSRTAYLGTHKTSAGISSGVTSGDSSDSAESSERRKRNRPITNGSTLSESEVEDSLATSLSSSASSSSEESKESSRARESSSRSGLSRSSNLRVTRTRAAQRKTGPVSLANGCGRKATRKRVYLSDSDNNSLETGEILKARAGNNRKVLRKCAAVAANKIKLMSDVEENSSSESVCSGRKLPHRNASAVARKKLLHNSEDEQSLKSEIEEEELKDENQLLPVSSSHTAQSNVDESENRDSESESDLRVARKNWHANGYKSHTPAPSKTKFLKIESSEEDSKSHDSDHACNRTAGPSTSVQKLKAESISEEADSEPGRSGGRKYNTFHKNASFFKKTKILSDSEDSESEEQDREDGKCHKMEMNPISGNLNCDPIAMSQCSSDHGCETDLDSDDDKIEKPNNFMKDSASQDNGLSRKISRKRVCSSDSDSSLQVVKKSSKARTGLLRITRRCAATAANKIKLMSDVEDVSLENVHTRSKNGRKKPLHLACTTAKKKLSDCEGSVHCEVPSEQYACEGKPPDPDSEGSTKVLSQALNGDSDSEDMLNSEHKHRHTNIHKIDAPSKRKSSSVTSSGEDSKSHIPGSETDRTFSSESTLAQKATAENNFEVELNYGLRRWNGRRLRTYGKAPFSKTKVIHDSQETAEKEVKRKRSHPELENVKISETTGNSKFRPDTSSKSSDLGSVTESDIDCTDNTKTKRRKTKGKAKVVRKEFVPRDREPNTKVRTCMHNQKDAVQMPSETLKAKMVPEKVPRRCATVAANKIKIMSNLKETISGPENVWIRKSSRKLPHRNASAAAKKKLLNVYKEDDTTINSESEKELEDINRKMLFLRGFRSWKENAQ

Target ORF information:

RefSeq Version	XM_011529611.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529611.1

ORF Insert Sequence:

ATGGCGGAGC CGTCGTCCGC CCGACGCCCG GTGCCTCTCA TCGAGTCGGA GCTGTACTTC 
CTTATCGCCC GGTACCTATC GGCGGGCCCG TGTCGGAGAG CGGCCCAGGT GCTGGTGCAG 
GAGCTGGAGC AGTACCAGTT GTTGCCGAAG AGATTGGACT GGGAGGGCAA CGAGCACAAC 
AGGAGCTACG AGGAGTTGGT CTTGTCCAAT AAGCATGTGG CTCCTGATCA TCTTTTGCAA 
ATCTGCCAGC GCATCGGTCC TATGTTGGAT AAAGAAATTC CACCCAGTAT TTCAAGAGTC 
ACTTCTTTAC TTGGTGCAGG AAGGCAGTCT TTGCTACGTA CAGCAAAAGA CTGCAGGCAC 
ACAGTTTGGA AGGGCTCTGC CTTTGCTGCT CTTCATAGAG GAAGACCTCC TGAAATGCCA 
GTGAATTATG GTTCCCCACC AAATCTTGTG GAGATACATC GAGGAAAACA ACTCACAGGG 
TGTTCCACTT TTAGTACAGC ATTTCCAGGA ACTATGTATC AGCATATAAA AATGCACAGA 
AGGATTCTCG GACATCTATC TGCTGTTTAC TGTGTAGCAT TTGATAGGAC AGGACATAGA 
ATCTTTACAG GTTCAGATGA CTGTTTGGTA AAGATTTGGT CAACACATAA TGGCCGCTTG 
TTATCTACAT TAAGAGGTCA TTCTGCAGAA ATTTCAGATA TGGCAGTAAA CTATGAGAAT 
ACAATGATTG CTGCGGGGAG CTGTGATAAA ATTATTAGAG TGTGGTGCTT GAGAACTTGT 
GCCCCAGTTG CTGTGCTCCA AGGACACACA GGATCAATTA CATCTTTACA GTTTAGCCCG 
ATGGCCAAAG GCTCTCAAAG ATACATGGTT TCCACTGGTG CTGATGGGAC AGTTTGCTTT 
TGGCAATGGG ATTTAGAATC CTTAAAATTT AGCCCACGTC CCCTGAAGTT CACTGAAAAG 
CCTAGGCCAG GCGTTCAAAT GCTTTGTTCT TCTTTTAGTG TTGGTGGTAT GTTTTTAGCC 
ACAGGTAGTA CTGATCATGT AATCAGAATG TATTTTTTGG GTTTTGAAGC ACCCGAAAAA 
ATCGCAGAAC TTGAAAGCCA CACTGATAAA GTAGATAGTA TCCAATTTTG TAACAATGGT 
GATCGGTTCC TAAGTGGTAG CAGAGATGGA ACAGCACGGA TTTGGAGATT TGAGCAGTTA 
GAATGGAGGA GCATTTTATT GGATATGGCT ACCAGAATCT CAGGGGACTT ATCTTCCGAA 
GAGGAAAGGT TTATGAAACC TAAAGTAACA ATGATAGCTT GGAATCAAAA TGATAGCATT 
GTTGTCACAG CTGTGAATGA TCATGTCCTC AAAGTGTGGA ATTCTTACAC TGGACAACTG 
CTTCATAACT TAATGGGACA TGCTGATGAA GTATTTGTTC TGGAGACACA TCCCTTTGAT 
TCCAGAATTA TGTTATCTGC AGGACATGAT GGCAGCATAT TTATATGGGA TATTACAAAA 
GGTACCAAGA TGAAACATTA TTTTAATATG ATTGAAGGAC AAGGACATGG AGCTGTGTTT 
GACTGTAAGT TTTCACAGGA TGGACAGCAT TTTGCCTGTA CAGATTCTCA TGGGCACCTT 
CTGATATTTG GTTTTGGATG CAGCAAACCA TATGAAAAGA TTCCTGATCA GATGTTCTTC 
CATACTGACT ATCGACCACT TATTAGAGAT TCTAATAATT ATGTCTTAGA TGAGCAAACT 
CAGCAGGCTC CTCATCTTAT GCCTCCACCA TTCTTGGTAG ATGTAGATGG AAATCCTCAT 
CCAACCAAGT ATCAGAGATT AGTACCAGGC CGAGAAAATT CTGCAGATGA ACATTTGATT 
CCACAGCTGG GCTATGTGGC AACAAGTGAT GGAGAGGTGA TTGAACAAAT TATAAGCCTG 
CAAACCAATG ATAATGATGA ACGCAGCCCA GAATCGAGTA TTCTTGATGG AATGATAAGA 
CAGTTGCAGC AGCAGCAAGA TCAGAGAATG GGAGCAGATC AGGATACTAT TCCAAGAGGA 
CTTTCAAATG GTGAAGAAAC ACCCCGGAGA GGTTTTAGAA GGCTGAGCTT AGACATTCAG 
TCCCCTCCAA ATATTGGTCT GCGTCGTAGT GGACAAGTTG AAGGTGTTCG TCAGATGCAT 
CAAAACGCTC CACGCAGTCA GATTGCTACA GAACGTGACC TGCAGGCTTG GAAACGAAGA 
GTGGTTGTAC CAGAGGTACC ACTAGGCATA TTTAGGAAGC TGGAAGACTT CCGATTAGAG 
AAAGGTGAAG AGGAAAGAAA TCTTTATATA ATAGGAAGAA AAAGAAAGAC TCTTCAGCTC 
TCACATAAGT CGGATTCAGT GGTTTTGGTA TCACAGTCTA GACAAAGGAC ATGTAGGCGT 
AAATATCCAA ATTATGGTAG AAGAAATCGT AGCTGGCGTG AGTTATCTTC TGGAAATGAG 
TCTTCAAGCT CTGTAAGACA TGAGACTTCC TGTGATCAGA GTGAAGGTTC TGGTTCTTCA 
GAAGAGGATG AATGGAGAAG TGACAGAAAA AGTGAGAGTT ACAGCGAAAG TTCAAGTGAC 
TCTTCATCTA GATATTCCGA TTGGACAGCT GATGCGGGCA TCAATTTGCA GCCTCCTTTA 
AGAACATCAT GTCGTCGACG AATTACTCGA TTTTGTAGTA GTTCAGAAGA TGAAATATCT 
ACTGAGAATT TATCTCCTCC AAAAAGAAGA CGAAAGAGAA AGAAAGAAAA TAAGCCTAAG 
AAGGAGAATT TGCGGAGGAT GACTCCAGCA GAGCTTGCAA ATATGGAGCA TTTATATGAA 
TTTCACCCTC CAGTTTGGAT TACTGACACC ACACTTAGAA AATCTCCTTT TGTTCCTCAA 
ATGGGTGATG AGGTAATATA TTTTCGACAG GGTCATGAAG CTTATATTGA GGCTGTAAGA 
AGAAATAATA TTTATGAACT GAACCCTAAT AAGGAGCCAT GGAGAAAAAT GGATCTTAGG 
GATCAAGAAT TGGTTAAAAT AGTTGGAATA CGATATGAAG TTGGGCCCCC TACACTCTGT 
TGCCTAAAAC TAGCATTTAT AGATCCAGCA ACTGGAAAAC TTATGGACAA ATCTTTCTCT 
ATTAGATATC ATGATATGCC AGATGTTATT GACTTTCTTG TATTGCGTCA ATTTTATGAT 
GAAGCAAGAC AGAGGAATTG GCAGTCTTGT GACAGATTCC GCTCTATTAT TGATGATGCT 
TGGTGGTTTG GAACAGTGTT AAGTCAAGAG CCATATCAAC CACAGTATCC TGATAGTCAT 
TTCCAGTGTT ATATTGTTAG GTGGGATAAT ACTGAAATTG AAAAACTTAG CCCATGGGAC 
ATGGAACCAA TTCCTGATAA TGTTGATCCA CCTGAAGAAT TAGGAGCTAG TATTTCTGTC 
ACAACAGATG AGCTAGAGAA ATTGCTCTAT AAACCACAAG CTGGTGAATG GGGTCAGAAA 
TCAAGAGATG AAGAATGTGA TAGAATTATC AGTGGTATAG ATCAACTTTT GAATCTTGAT 
ATAGCAGCAG CTTTTGCAGG CCCTGTTGAT TTGTGTACAT ACCCGAAGTA CTGTACTGTA 
GTAGCTTATC CAACCGATCT TTACACAATT CGAATGAGAC TTGTTAATCG ATTTTACAGG 
AGGCTGTCTG CGTTAGTTTG GGAAGTCAGA TATATAGAAC ATAATGCCAG AACATTTAAC 
GAACCTGAGA GTGTAATTGC AAGATCAGCT AAAAAGATAA CTGACCAACT TTTAAAATTT 
ATCAAGAATC AACACTGTAC AAATATCTCA GAACTTTCTA ACACATCTGA AAATGATGAG 
CAAAATGCTG AGGATTTGGA TGATAGTGAT CTTCCTAAAA CATCTTCTGG AAGGAGGAGA 
GACTACAGAG ATATTATAGA TACCCCAATG GATTTTGGAA CAGTAAGGGA AACTCTAGAT 
GCGGGAAATT ATGACAGCCC TTTGGAGTTT TGCAAAGACA TCCGGCTGAT ATTTAGCAAT 
GCAAAAGCGT ATACACCAAA CAAAAGATCA AAGATTTATA GTATGACCTT GAGATTATCT 
GCCTTATTTG AAGAAAAAAT GAAGAAAATC TCTTCTGATT TTAAAATTGG TCAAAAATTC 
AATGAAAAAC TTCGAAGAAG CCAGAGGTTC AAGCAACGGC AAAATTGTAA AGGTGACAGT 
CAGCCTAACA AAAGTATCAG AAACCTCAAG CCGAAGAGGT TAAAATCTCA GACAAAAATA 
ATTCCTGAGT TGGTAGGTTC TCCTACCCAG TCTACCTCAA GTAGGACAGC TTATCTTGGA 
ACCCACAAGA CAAGTGCTGG TATCTCTTCA GGTGTTACTT CTGGTGACTC TTCAGATTCA 
GCAGAATCAT CAGAAAGGAG GAAAAGAAAT AGACCTATAA CAAATGGTTC TACATTATCT 
GAAAGTGAAG TGGAAGATTC TTTAGCTACC TCTTTGTCAT CGTCAGCTTC CAGTAGTTCT 
GAGGAAAGCA AAGAGAGTTC CAGAGCTCGT GAATCCTCCT CACGCAGTGG GCTATCCAGA 
AGCAGCAATC TCAGGGTAAC CAGAACTAGA GCTGCTCAAA GAAAAACTGG TCCCGTTTCA 
TTAGCAAATG GATGTGGCAG AAAAGCCACT CGAAAGAGAG TCTATTTAAG TGATTCTGAT 
AACAATTCAT TGGAGACTGG TGAAATTCTA AAAGCCAGAG CTGGAAATAA CCGAAAAGTC 
TTAAGGAAGT GTGCTGCTGT GGCTGCCAAT AAAATAAAGC TAATGAGTGA TGTAGAAGAG 
AATTCTAGCT CTGAAAGTGT CTGTTCTGGT CGGAAGCTGC CTCACCGCAA TGCTTCTGCT 
GTAGCTAGAA AAAAGTTATT ACATAATTCT GAAGATGAAC AGAGCTTAAA GTCAGAAATT 
GAAGAAGAGG AGCTAAAAGA TGAAAATCAA CTATTACCAG TGTCCAGTTC TCACACTGCC 
CAGAGCAATG TTGATGAATC TGAAAACAGA GACTCAGAGT CAGAAAGTGA TTTGCGGGTA 
GCCCGGAAAA ATTGGCATGC TAATGGTTAC AAGTCCCATA CTCCAGCACC TTCAAAGACA 
AAATTTCTTA AAATAGAGTC TTCTGAGGAA GACTCTAAAA GTCATGATTC AGATCATGCA 
TGTAACAGAA CTGCTGGCCC ATCAACGTCT GTGCAGAAAC TTAAGGCAGA GAGCATCTCA 
GAGGAAGCAG ATTCTGAACC AGGAAGATCT GGTGGTAGGA AATACAATAC ATTTCACAAG 
AATGCGAGTT TCTTTAAAAA AACCAAGATT CTGAGTGACT CAGAAGACTC TGAATCTGAA 
GAGCAAGATA GAGAAGATGG GAAATGTCAT AAAATGGAAA TGAACCCAAT TTCAGGAAAT 
CTGAACTGTG ACCCTATTGC TATGTCCCAG TGTTCCTCAG ATCATGGATG TGAAACTGAT 
TTAGATTCAG ATGATGACAA AATAGAAAAA CCAAACAATT TTATGAAAGA TTCTGCATCA 
CAAGACAATG GACTAAGCAG AAAAATTTCC AGGAAAAGGG TCTGTTCCAG TGACTCAGAC 
AGTAGTTTAC AGGTGGTTAA GAAATCATCA AAAGCCAGAA CAGGTCTCCT GAGGATTACT 
CGAAGATGTG CAGCTACGGC TGCCAATAAG ATCAAGCTCA TGAGTGATGT AGAAGATGTC 
AGTTTAGAAA ATGTGCACAC TAGAAGCAAA AATGGAAGGA AAAAACCTCT CCATCTTGCT 
TGTACTACAG CTAAGAAGAA ATTGAGTGAT TGTGAAGGAA GTGTACATTG TGAAGTACCA 
AGTGAACAGT ATGCCTGTGA AGGCAAGCCA CCTGATCCTG ACTCCGAAGG TAGTACAAAA 
GTGCTTAGTC AGGCTCTAAA TGGAGACTCA GACTCTGAAG ATATGTTGAA TTCAGAACAC 
AAGCACAGGC ATACCAATAT TCACAAAATA GATGCACCTT CTAAAAGAAA AAGTTCCTCT 
GTTACATCTT CAGGAGAAGA TTCAAAAAGT CATATTCCAG GGAGTGAGAC TGATAGGACA 
TTTTCTTCAG AGTCAACCTT GGCACAAAAA GCTACTGCAG AGAATAATTT TGAAGTGGAA 
CTGAATTATG GGCTGCGCAG GTGGAATGGC AGAAGACTCA GGACCTATGG AAAGGCTCCT 
TTTAGTAAGA CAAAAGTGAT TCATGATTCA CAGGAAACAG CAGAGAAGGA AGTAAAAAGG 
AAGAGATCGC ATCCTGAATT GGAAAATGTG AAAATCTCTG AAACAACTGG GAATTCAAAG 
TTTAGACCTG ATACTAGTTC CAAATCATCA GATTTGGGAT CTGTAACTGA ATCAGATATT 
GACTGTACTG ATAATACAAA AACCAAAAGG AGGAAAACGA AAGGAAAAGC AAAAGTAGTT 
AGAAAAGAAT TTGTTCCTAG AGACAGAGAA CCCAATACAA AAGTGAGAAC ATGTATGCAT 
AATCAGAAGG ATGCAGTGCA GATGCCTAGT GAAACTCTGA AAGCAAAAAT GGTACCTGAG 
AAAGTTCCCC GCAGATGTGC TACTGTTGCT GCAAATAAAA TAAAGATAAT GAGTAATCTA 
AAAGAAACGA TCTCAGGACC AGAAAATGTC TGGATTAGGA AGAGTAGCAG AAAACTGCCC 
CATCGAAATG CTTCTGCTGC GGCTAAGAAA AAATTACTGA ATGTTTATAA AGAGGATGAT 
ACAACCATAA ATTCTGAAAG TGAAAAAGAG CTAGAAGATA TTAATAGAAA AATGCTTTTT 
TTAAGGGGGT TCAGATCCTG GAAAGAAAAT GCACAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRWD1 ( XM_011529612.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527914.1 Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant X3, mRNA.

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CloneID	OHu68248
Clone ID Related Accession (Same CDS sequence)	XM_011529612.2
Accession Version	XM_011529612.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bromodomain and WD repeat-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011529612.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG GCTCTCAAAG ATACATGGTT TCCACTGGTG CTGATGGGAC AGTTTGCTTT 
TGGCAATGGG ATTTAGAATC CTTAAAATTT AGCCCACGTC CCCTGAAGTT CACTGAAAAG 
CCTAGGCCAG GCGTTCAAAT GCTTTGTTCT TCTTTTAGTG TTGGTGGTAT GTTTTTAGCC 
ACAGGTAGTA CTGATCATGT AATCAGAATG TATTTTTTGG GTTTTGAAGC ACCCGAAAAA 
ATCGCAGAAC TTGAAAGCCA CACTGATAAA GTAGATAGTA TCCAATTTTG TAACAATGGT 
GATCGGTTCC TAAGTGGTAG CAGAGATGGA ACAGCACGGA TTTGGAGATT TGAGCAGTTA 
GAATGGAGGA GCATTTTATT GGATATGGCT ACCAGAATCT CAGGGGACTT ATCTTCCGAA 
GAGGAAAGGT TTATGAAACC TAAAGTAACA ATGATAGCTT GGAATCAAAA TGATAGCATT 
GTTGTCACAG CTGTGAATGA TCATGTCCTC AAAGTGTGGA ATTCTTACAC TGGACAACTG 
CTTCATAACT TAATGGGACA TGCTGATGAA GTATTTGTTC TGGAGACACA TCCCTTTGAT 
TCCAGAATTA TGTTATCTGC AGGACATGAT GGCAGCATAT TTATATGGGA TATTACAAAA 
GGTACCAAGA TGAAACATTA TTTTAATATG ATTGAAGGAC AAGGACATGG AGCTGTGTTT 
GACTGTAAGT TTTCACAGGA TGGACAGCAT TTTGCCTGTA CAGATTCTCA TGGGCACCTT 
CTGATATTTG GTTTTGGATG CAGCAAACCA TATGAAAAGA TTCCTGATCA GATGTTCTTC 
CATACTGACT ATCGACCACT TATTAGAGAT TCTAATAATT ATGTCTTAGA TGAGCAAACT 
CAGCAGGCTC CTCATCTTAT GCCTCCACCA TTCTTGGTAG ATGTAGATGG AAATCCTCAT 
CCAACCAAGT ATCAGAGATT AGTACCAGGC CGAGAAAATT CTGCAGATGA ACATTTGATT 
CCACAGCTGG GCTATGTGGC AACAAGTGAT GGAGAGGTGA TTGAACAAAT TATAAGCCTG 
CAAACCAATG ATAATGATGA ACGCAGCCCA GAATCGAGTA TTCTTGATGG AATGATAAGA 
CAGTTGCAGC AGCAGCAAGA TCAGAGAATG GGAGCAGATC AGGATACTAT TCCAAGAGGA 
CTTTCAAATG GTGAAGAAAC ACCCCGGAGA GGTTTTAGAA GGCTGAGCTT AGACATTCAG 
TCCCCTCCAA ATATTGGTCT GCGTCGTAGT GGACAAGTTG AAGGTGTTCG TCAGATGCAT 
CAAAACGCTC CACGCAGTCA GATTGCTACA GAACGTGACC TGCAGGCTTG GAAACGAAGA 
GTGGTTGTAC CAGAGGTACC ACTAGGCATA TTTAGGAAGC TGGAAGACTT CCGATTAGAG 
AAAGGTGAAG AGGAAAGAAA TCTTTATATA ATAGGAAGAA AAAGAAAGAC TCTTCAGCTC 
TCACATAAGT CGGATTCAGT GGTTTTGGTA TCACAGTCTA GACAAAGGAC ATGTAGGCGT 
AAATATCCAA ATTATGGTAG AAGAAATCGT AGCTGGCGTG AGTTATCTTC TGGAAATGAG 
TCTTCAAGCT CTGTAAGACA TGAGACTTCC TGTGATCAGA GTGAAGGTTC TGGTTCTTCA 
GAAGAGGATG AATGGAGAAG TGACAGAAAA AGTGAGAGTT ACAGCGAAAG TTCAAGTGAC 
TCTTCATCTA GATATTCCGA TTGGACAGCT GATGCGGGCA TCAATTTGCA GCCTCCTTTA 
AGAACATCAT GTCGTCGACG AATTACTCGA TTTTGTAGTA GTTCAGAAGA TGAAATATCT 
ACTGAGAATT TATCTCCTCC AAAAAGAAGA CGAAAGAGAA AGAAAGAAAA TAAGCCTAAG 
AAGGAGAATT TGCGGAGGAT GACTCCAGCA GAGCTTGCAA ATATGGAGCA TTTATATGAA 
TTTCACCCTC CAGTTTGGAT TACTGACACC ACACTTAGAA AATCTCCTTT TGTTCCTCAA 
ATGGGTGATG AGGTAATATA TTTTCGACAG GGTCATGAAG CTTATATTGA GGCTGTAAGA 
AGAAATAATA TTTATGAACT GAACCCTAAT AAGGAGCCAT GGAGAAAAAT GGATCTTAGG 
GATCAAGAAT TGGTTAAAAT AGTTGGAATA CGATATGAAG TTGGGCCCCC TACACTCTGT 
TGCCTAAAAC TAGCATTTAT AGATCCAGCA ACTGGAAAAC TTATGGACAA ATCTTTCTCT 
ATTAGATATC ATGATATGCC AGATGTTATT GACTTTCTTG TATTGCGTCA ATTTTATGAT 
GAAGCAAGAC AGAGGAATTG GCAGTCTTGT GACAGATTCC GCTCTATTAT TGATGATGCT 
TGGTGGTTTG GAACAGTGTT AAGTCAAGAG CCATATCAAC CACAGTATCC TGATAGTCAT 
TTCCAGTGTT ATATTGTTAG GTGGGATAAT ACTGAAATTG AAAAACTTAG CCCATGGGAC 
ATGGAACCAA TTCCTGATAA TGTTGATCCA CCTGAAGAAT TAGGAGCTAG TATTTCTGTC 
ACAACAGATG AGCTAGAGAA ATTGCTCTAT AAACCACAAG CTGGTGAATG GGGTCAGAAA 
TCAAGAGATG AAGAATGTGA TAGAATTATC AGTGGTATAG ATCAACTTTT GAATCTTGAT 
ATAGCAGCAG CTTTTGCAGG CCCTGTTGAT TTGTGTACAT ACCCGAAGTA CTGTACTGTA 
GTAGCTTATC CAACCGATCT TTACACAATT CGAATGAGAC TTGTTAATCG ATTTTACAGG 
AGGCTGTCTG CGTTAGTTTG GGAAGTCAGA TATATAGAAC ATAATGCCAG AACATTTAAC 
GAACCTGAGA GTGTAATTGC AAGATCAGCT AAAAAGATAA CTGACCAACT TTTAAAATTT 
ATCAAGAATC AACACTGTAC AAATATCTCA GAACTTTCTA ACACATCTGA AAATGATGAG 
CAAAATGCTG AGGATTTGGA TGATAGTGAT CTTCCTAAAA CATCTTCTGG AAGGAGGAGA 
GTCCATGATG GGAAAAAAAG CATCAGAGCT ACGAACTATG TTGAAAGCAA CTGGAAGAAA 
CAGTGTAAGG AACTAGTGAA CTTAATTTTT CAGTGTGAAG ATTCTGAACC ATTTAGACAA 
CCTGTTGATT TGGTTGAATA TCCAGACTAC AGAGATATTA TAGATACCCC AATGGATTTT 
GGAACAGTAA GGGAAACTCT AGATGCGGGA AATTATGACA GCCCTTTGGA GTTTTGCAAA 
GACATCCGGC TGATATTTAG CAATGCAAAA GCGTATACAC CAAACAAAAG ATCAAAGATT 
TATAGTATGA CCTTGAGATT ATCTGCCTTA TTTGAAGAAA AAATGAAGAA AATCTCTTCT 
GATTTTAAAA TTGGTCAAAA ATTCAATGAA AAACTTCGAA GAAGCCAGAG GTTCAAGCAA 
CGGCAAAATT GTAAAGGTGA CAGTCAGCCT AACAAAAGTA TCAGAAACCT CAAGCCGAAG 
AGGTTAAAAT CTCAGACAAA AATAATTCCT GAGTTGGTAG GTTCTCCTAC CCAGTCTACC 
TCAAGTAGGA CAGCTTATCT TGGAACCCAC AAGACAAGTG CTGGTATCTC TTCAGGTGTT 
ACTTCTGGTG ACTCTTCAGA TTCAGCAGAA TCATCAGAAA GGAGGAAAAG AAATAGACCT 
ATAACAAATG GTTCTACATT ATCTGAAAGT GAAGTGGAAG ATTCTTTAGC TACCTCTTTG 
TCATCGTCAG CTTCCAGTAG TTCTGAGGAA AGCAAAGAGA GTTCCAGAGC TCGTGAATCC 
TCCTCACGCA GTGGGCTATC CAGAAGCAGC AATCTCAGGG TAACCAGAAC TAGAGCTGCT 
CAAAGAAAAA CTGGTCCCGT TTCATTAGCA AATGGATGTG GCAGAAAAGC CACTCGAAAG 
AGAGTCTATT TAAGTGATTC TGATAACAAT TCATTGGAGA CTGGTGAAAT TCTAAAAGCC 
AGAGCTGGAA ATAACCGAAA AGTCTTAAGG AAGTGTGCTG CTGTGGCTGC CAATAAAATA 
AAGCTAATGA GTGATGTAGA AGAGAATTCT AGCTCTGAAA GTGTCTGTTC TGGTCGGAAG 
CTGCCTCACC GCAATGCTTC TGCTGTAGCT AGAAAAAAGT TATTACATAA TTCTGAAGAT 
GAACAGAGCT TAAAGTCAGA AATTGAAGAA GAGGAGCTAA AAGATGAAAA TCAACTATTA 
CCAGTGTCCA GTTCTCACAC TGCCCAGAGC AATGTTGATG AATCTGAAAA CAGAGACTCA 
GAGTCAGAAA GTGATTTGCG GGTAGCCCGG AAAAATTGGC ATGCTAATGG TTACAAGTCC 
CATACTCCAG CACCTTCAAA GACAAAATTT CTTAAAATAG AGTCTTCTGA GGAAGACTCT 
AAAAGTCATG ATTCAGATCA TGCATGTAAC AGAACTGCTG GCCCATCAAC GTCTGTGCAG 
AAACTTAAGG CAGAGAGCAT CTCAGAGGAA GCAGATTCTG AACCAGGAAG ATCTGGTGGT 
AGGAAATACA ATACATTTCA CAAGAATGCG AGTTTCTTTA AAAAAACCAA GATTCTGAGT 
GACTCAGAAG ACTCTGAATC TGAAGAGCAA GATAGAGAAG ATGGGAAATG TCATAAAATG 
GAAATGAACC CAATTTCAGG AAATCTGAAC TGTGACCCTA TTGCTATGTC CCAGTGTTCC 
TCAGATCATG GATGTGAAAC TGATTTAGAT TCAGATGATG ACAAAATAGA AAAACCAAAC 
AATTTTATGA AAGATTCTGC ATCACAAGAC AATGGACTAA GCAGAAAAAT TTCCAGGAAA 
AGGGTCTGTT CCAGTGACTC AGACAGTAGT TTACAGGTGG TTAAGAAATC ATCAAAAGCC 
AGAACAGGTC TCCTGAGGAT TACTCGAAGA TGTGCAGCTA CGGCTGCCAA TAAGATCAAG 
CTCATGAGTG ATGTAGAAGA TGTCAGTTTA GAAAATGTGC ACACTAGAAG CAAAAATGGA 
AGGAAAAAAC CTCTCCATCT TGCTTGTACT ACAGCTAAGA AGAAATTGAG TGATTGTGAA 
GGAAGTGTAC ATTGTGAAGT ACCAAGTGAA CAGTATGCCT GTGAAGGCAA GCCACCTGAT 
CCTGACTCCG AAGGTAGTAC AAAAGTGCTT AGTCAGGCTC TAAATGGAGA CTCAGACTCT 
GAAGATATGT TGAATTCAGA ACACAAGCAC AGGCATACCA ATATTCACAA AATAGATGCA 
CCTTCTAAAA GAAAAAGTTC CTCTGTTACA TCTTCAGGAG AAGATTCAAA AAGTCATATT 
CCAGGGAGTG AGACTGATAG GACATTTTCT TCAGAGTCAA CCTTGGCACA AAAAGCTACT 
GCAGAGAATA ATTTTGAAGT GGAACTGAAT TATGGGCTGC GCAGGTGGAA TGGCAGAAGA 
CTCAGGACCT ATGGAAAGGC TCCTTTTAGT AAGACAAAAG TGATTCATGA TTCACAGGAA 
ACAGCAGAGA AGGAAGTAAA AAGGAAGAGA TCGCATCCTG AATTGGAAAA TGTGAAAATC 
TCTGAAACAA CTGGGAATTC AAAGTTTAGA CCTGATACTA GTTCCAAATC ATCAGATTTG 
GGATCTGTAA CTGAATCAGA TATTGACTGT ACTGATAATA CAAAAACCAA AAGGAGGAAA 
ACGAAAGGAA AAGCAAAAGT AGTTAGAAAA GAATTTGTTC CTAGAGACAG AGAACCCAAT 
ACAAAAGTGA GAACATGTAT GCATAATCAG AAGGATGCAG TGCAGATGCC TAGTGAAACT 
CTGAAAGCAA AAATGGTACC TGAGAAAGTT CCCCGCAGAT GTGCTACTGT TGCTGCAAAT 
AAAATAAAGA TAATGAGTAA TCTAAAAGAA ACGATCTCAG GACCAGAAAA TGTCTGGATT 
AGGAAGAGTA GCAGAAAACT GCCCCATCGA AATGCTTCTG CTGCGGCTAA GAAAAAATTA 
CTGAATGTTT ATAAAGAGGA TGATACAACC ATAAATTCTG AAAGTGAAAA AGAGCTAGAA 
GATATTAATA GAAAAATGCT TTTTTTAAGG GGGTTCAGAT CCTGGAAAGA AAATGCACAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527914.1
CDS	32..6154
Translation	MAKGSQRYMVSTGADGTVCFWQWDLESLKFSPRPLKFTEKPRPGVQMLCSSFSVGGMFLATGSTDHVIRMYFLGFEAPEKIAELESHTDKVDSIQFCNNGDRFLSGSRDGTARIWRFEQLEWRSILLDMATRISGDLSSEEERFMKPKVTMIAWNQNDSIVVTAVNDHVLKVWNSYTGQLLHNLMGHADEVFVLETHPFDSRIMLSAGHDGSIFIWDITKGTKMKHYFNMIEGQGHGAVFDCKFSQDGQHFACTDSHGHLLIFGFGCSKPYEKIPDQMFFHTDYRPLIRDSNNYVLDEQTQQAPHLMPPPFLVDVDGNPHPTKYQRLVPGRENSADEHLIPQLGYVATSDGEVIEQIISLQTNDNDERSPESSILDGMIRQLQQQQDQRMGADQDTIPRGLSNGEETPRRGFRRLSLDIQSPPNIGLRRSGQVEGVRQMHQNAPRSQIATERDLQAWKRRVVVPEVPLGIFRKLEDFRLEKGEEERNLYIIGRKRKTLQLSHKSDSVVLVSQSRQRTCRRKYPNYGRRNRSWRELSSGNESSSSVRHETSCDQSEGSGSSEEDEWRSDRKSESYSESSSDSSSRYSDWTADAGINLQPPLRTSCRRRITRFCSSSEDEISTENLSPPKRRRKRKKENKPKKENLRRMTPAELANMEHLYEFHPPVWITDTTLRKSPFVPQMGDEVIYFRQGHEAYIEAVRRNNIYELNPNKEPWRKMDLRDQELVKIVGIRYEVGPPTLCCLKLAFIDPATGKLMDKSFSIRYHDMPDVIDFLVLRQFYDEARQRNWQSCDRFRSIIDDAWWFGTVLSQEPYQPQYPDSHFQCYIVRWDNTEIEKLSPWDMEPIPDNVDPPEELGASISVTTDELEKLLYKPQAGEWGQKSRDEECDRIISGIDQLLNLDIAAAFAGPVDLCTYPKYCTVVAYPTDLYTIRMRLVNRFYRRLSALVWEVRYIEHNARTFNEPESVIARSAKKITDQLLKFIKNQHCTNISELSNTSENDEQNAEDLDDSDLPKTSSGRRRVHDGKKSIRATNYVESNWKKQCKELVNLIFQCEDSEPFRQPVDLVEYPDYRDIIDTPMDFGTVRETLDAGNYDSPLEFCKDIRLIFSNAKAYTPNKRSKIYSMTLRLSALFEEKMKKISSDFKIGQKFNEKLRRSQRFKQRQNCKGDSQPNKSIRNLKPKRLKSQTKIIPELVGSPTQSTSSRTAYLGTHKTSAGISSGVTSGDSSDSAESSERRKRNRPITNGSTLSESEVEDSLATSLSSSASSSSEESKESSRARESSSRSGLSRSSNLRVTRTRAAQRKTGPVSLANGCGRKATRKRVYLSDSDNNSLETGEILKARAGNNRKVLRKCAAVAANKIKLMSDVEENSSSESVCSGRKLPHRNASAVARKKLLHNSEDEQSLKSEIEEEELKDENQLLPVSSSHTAQSNVDESENRDSESESDLRVARKNWHANGYKSHTPAPSKTKFLKIESSEEDSKSHDSDHACNRTAGPSTSVQKLKAESISEEADSEPGRSGGRKYNTFHKNASFFKKTKILSDSEDSESEEQDREDGKCHKMEMNPISGNLNCDPIAMSQCSSDHGCETDLDSDDDKIEKPNNFMKDSASQDNGLSRKISRKRVCSSDSDSSLQVVKKSSKARTGLLRITRRCAATAANKIKLMSDVEDVSLENVHTRSKNGRKKPLHLACTTAKKKLSDCEGSVHCEVPSEQYACEGKPPDPDSEGSTKVLSQALNGDSDSEDMLNSEHKHRHTNIHKIDAPSKRKSSSVTSSGEDSKSHIPGSETDRTFSSESTLAQKATAENNFEVELNYGLRRWNGRRLRTYGKAPFSKTKVIHDSQETAEKEVKRKRSHPELENVKISETTGNSKFRPDTSSKSSDLGSVTESDIDCTDNTKTKRRKTKGKAKVVRKEFVPRDREPNTKVRTCMHNQKDAVQMPSETLKAKMVPEKVPRRCATVAANKIKIMSNLKETISGPENVWIRKSSRKLPHRNASAAAKKKLLNVYKEDDTTINSESEKELEDINRKMLFLRGFRSWKENAQ

Target ORF information:

RefSeq Version	XM_011529612.2
Organism	Homo sapiens(human)
Definition	Homo sapiens bromodomain and WD repeat domain containing 1 (BRWD1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529612.2

ORF Insert Sequence:

ATGGCCAAAG GCTCTCAAAG ATACATGGTT TCCACTGGTG CTGATGGGAC AGTTTGCTTT 
TGGCAATGGG ATTTAGAATC CTTAAAATTT AGCCCACGTC CCCTGAAGTT CACTGAAAAG 
CCTAGGCCAG GCGTTCAAAT GCTTTGTTCT TCTTTTAGTG TTGGTGGTAT GTTTTTAGCC 
ACAGGTAGTA CTGATCATGT AATCAGAATG TATTTTTTGG GTTTTGAAGC ACCCGAAAAA 
ATCGCAGAAC TTGAAAGCCA CACTGATAAA GTAGATAGTA TCCAATTTTG TAACAATGGT 
GATCGGTTCC TAAGTGGTAG CAGAGATGGA ACAGCACGGA TTTGGAGATT TGAGCAGTTA 
GAATGGAGGA GCATTTTATT GGATATGGCT ACCAGAATCT CAGGGGACTT ATCTTCCGAA 
GAGGAAAGGT TTATGAAACC TAAAGTAACA ATGATAGCTT GGAATCAAAA TGATAGCATT 
GTTGTCACAG CTGTGAATGA TCATGTCCTC AAAGTGTGGA ATTCTTACAC TGGACAACTG 
CTTCATAACT TAATGGGACA TGCTGATGAA GTATTTGTTC TGGAGACACA TCCCTTTGAT 
TCCAGAATTA TGTTATCTGC AGGACATGAT GGCAGCATAT TTATATGGGA TATTACAAAA 
GGTACCAAGA TGAAACATTA TTTTAATATG ATTGAAGGAC AAGGACATGG AGCTGTGTTT 
GACTGTAAGT TTTCACAGGA TGGACAGCAT TTTGCCTGTA CAGATTCTCA TGGGCACCTT 
CTGATATTTG GTTTTGGATG CAGCAAACCA TATGAAAAGA TTCCTGATCA GATGTTCTTC 
CATACTGACT ATCGACCACT TATTAGAGAT TCTAATAATT ATGTCTTAGA TGAGCAAACT 
CAGCAGGCTC CTCATCTTAT GCCTCCACCA TTCTTGGTAG ATGTAGATGG AAATCCTCAT 
CCAACCAAGT ATCAGAGATT AGTACCAGGC CGAGAAAATT CTGCAGATGA ACATTTGATT 
CCACAGCTGG GCTATGTGGC AACAAGTGAT GGAGAGGTGA TTGAACAAAT TATAAGCCTG 
CAAACCAATG ATAATGATGA ACGCAGCCCA GAATCGAGTA TTCTTGATGG AATGATAAGA 
CAGTTGCAGC AGCAGCAAGA TCAGAGAATG GGAGCAGATC AGGATACTAT TCCAAGAGGA 
CTTTCAAATG GTGAAGAAAC ACCCCGGAGA GGTTTTAGAA GGCTGAGCTT AGACATTCAG 
TCCCCTCCAA ATATTGGTCT GCGTCGTAGT GGACAAGTTG AAGGTGTTCG TCAGATGCAT 
CAAAACGCTC CACGCAGTCA GATTGCTACA GAACGTGACC TGCAGGCTTG GAAACGAAGA 
GTGGTTGTAC CAGAGGTACC ACTAGGCATA TTTAGGAAGC TGGAAGACTT CCGATTAGAG 
AAAGGTGAAG AGGAAAGAAA TCTTTATATA ATAGGAAGAA AAAGAAAGAC TCTTCAGCTC 
TCACATAAGT CGGATTCAGT GGTTTTGGTA TCACAGTCTA GACAAAGGAC ATGTAGGCGT 
AAATATCCAA ATTATGGTAG AAGAAATCGT AGCTGGCGTG AGTTATCTTC TGGAAATGAG 
TCTTCAAGCT CTGTAAGACA TGAGACTTCC TGTGATCAGA GTGAAGGTTC TGGTTCTTCA 
GAAGAGGATG AATGGAGAAG TGACAGAAAA AGTGAGAGTT ACAGCGAAAG TTCAAGTGAC 
TCTTCATCTA GATATTCCGA TTGGACAGCT GATGCGGGCA TCAATTTGCA GCCTCCTTTA 
AGAACATCAT GTCGTCGACG AATTACTCGA TTTTGTAGTA GTTCAGAAGA TGAAATATCT 
ACTGAGAATT TATCTCCTCC AAAAAGAAGA CGAAAGAGAA AGAAAGAAAA TAAGCCTAAG 
AAGGAGAATT TGCGGAGGAT GACTCCAGCA GAGCTTGCAA ATATGGAGCA TTTATATGAA 
TTTCACCCTC CAGTTTGGAT TACTGACACC ACACTTAGAA AATCTCCTTT TGTTCCTCAA 
ATGGGTGATG AGGTAATATA TTTTCGACAG GGTCATGAAG CTTATATTGA GGCTGTAAGA 
AGAAATAATA TTTATGAACT GAACCCTAAT AAGGAGCCAT GGAGAAAAAT GGATCTTAGG 
GATCAAGAAT TGGTTAAAAT AGTTGGAATA CGATATGAAG TTGGGCCCCC TACACTCTGT 
TGCCTAAAAC TAGCATTTAT AGATCCAGCA ACTGGAAAAC TTATGGACAA ATCTTTCTCT 
ATTAGATATC ATGATATGCC AGATGTTATT GACTTTCTTG TATTGCGTCA ATTTTATGAT 
GAAGCAAGAC AGAGGAATTG GCAGTCTTGT GACAGATTCC GCTCTATTAT TGATGATGCT 
TGGTGGTTTG GAACAGTGTT AAGTCAAGAG CCATATCAAC CACAGTATCC TGATAGTCAT 
TTCCAGTGTT ATATTGTTAG GTGGGATAAT ACTGAAATTG AAAAACTTAG CCCATGGGAC 
ATGGAACCAA TTCCTGATAA TGTTGATCCA CCTGAAGAAT TAGGAGCTAG TATTTCTGTC 
ACAACAGATG AGCTAGAGAA ATTGCTCTAT AAACCACAAG CTGGTGAATG GGGTCAGAAA 
TCAAGAGATG AAGAATGTGA TAGAATTATC AGTGGTATAG ATCAACTTTT GAATCTTGAT 
ATAGCAGCAG CTTTTGCAGG CCCTGTTGAT TTGTGTACAT ACCCGAAGTA CTGTACTGTA 
GTAGCTTATC CAACCGATCT TTACACAATT CGAATGAGAC TTGTTAATCG ATTTTACAGG 
AGGCTGTCTG CGTTAGTTTG GGAAGTCAGA TATATAGAAC ATAATGCCAG AACATTTAAC 
GAACCTGAGA GTGTAATTGC AAGATCAGCT AAAAAGATAA CTGACCAACT TTTAAAATTT 
ATCAAGAATC AACACTGTAC AAATATCTCA GAACTTTCTA ACACATCTGA AAATGATGAG 
CAAAATGCTG AGGATTTGGA TGATAGTGAT CTTCCTAAAA CATCTTCTGG AAGGAGGAGA 
GTCCATGATG GGAAAAAAAG CATCAGAGCT ACGAACTATG TTGAAAGCAA CTGGAAGAAA 
CAGTGTAAGG AACTAGTGAA CTTAATTTTT CAGTGTGAAG ATTCTGAACC ATTTAGACAA 
CCTGTTGATT TGGTTGAATA TCCAGACTAC AGAGATATTA TAGATACCCC AATGGATTTT 
GGAACAGTAA GGGAAACTCT AGATGCGGGA AATTATGACA GCCCTTTGGA GTTTTGCAAA 
GACATCCGGC TGATATTTAG CAATGCAAAA GCGTATACAC CAAACAAAAG ATCAAAGATT 
TATAGTATGA CCTTGAGATT ATCTGCCTTA TTTGAAGAAA AAATGAAGAA AATCTCTTCT 
GATTTTAAAA TTGGTCAAAA ATTCAATGAA AAACTTCGAA GAAGCCAGAG GTTCAAGCAA 
CGGCAAAATT GTAAAGGTGA CAGTCAGCCT AACAAAAGTA TCAGAAACCT CAAGCCGAAG 
AGGTTAAAAT CTCAGACAAA AATAATTCCT GAGTTGGTAG GTTCTCCTAC CCAGTCTACC 
TCAAGTAGGA CAGCTTATCT TGGAACCCAC AAGACAAGTG CTGGTATCTC TTCAGGTGTT 
ACTTCTGGTG ACTCTTCAGA TTCAGCAGAA TCATCAGAAA GGAGGAAAAG AAATAGACCT 
ATAACAAATG GTTCTACATT ATCTGAAAGT GAAGTGGAAG ATTCTTTAGC TACCTCTTTG 
TCATCGTCAG CTTCCAGTAG TTCTGAGGAA AGCAAAGAGA GTTCCAGAGC TCGTGAATCC 
TCCTCACGCA GTGGGCTATC CAGAAGCAGC AATCTCAGGG TAACCAGAAC TAGAGCTGCT 
CAAAGAAAAA CTGGTCCCGT TTCATTAGCA AATGGATGTG GCAGAAAAGC CACTCGAAAG 
AGAGTCTATT TAAGTGATTC TGATAACAAT TCATTGGAGA CTGGTGAAAT TCTAAAAGCC 
AGAGCTGGAA ATAACCGAAA AGTCTTAAGG AAGTGTGCTG CTGTGGCTGC CAATAAAATA 
AAGCTAATGA GTGATGTAGA AGAGAATTCT AGCTCTGAAA GTGTCTGTTC TGGTCGGAAG 
CTGCCTCACC GCAATGCTTC TGCTGTAGCT AGAAAAAAGT TATTACATAA TTCTGAAGAT 
GAACAGAGCT TAAAGTCAGA AATTGAAGAA GAGGAGCTAA AAGATGAAAA TCAACTATTA 
CCAGTGTCCA GTTCTCACAC TGCCCAGAGC AATGTTGATG AATCTGAAAA CAGAGACTCA 
GAGTCAGAAA GTGATTTGCG GGTAGCCCGG AAAAATTGGC ATGCTAATGG TTACAAGTCC 
CATACTCCAG CACCTTCAAA GACAAAATTT CTTAAAATAG AGTCTTCTGA GGAAGACTCT 
AAAAGTCATG ATTCAGATCA TGCATGTAAC AGAACTGCTG GCCCATCAAC GTCTGTGCAG 
AAACTTAAGG CAGAGAGCAT CTCAGAGGAA GCAGATTCTG AACCAGGAAG ATCTGGTGGT 
AGGAAATACA ATACATTTCA CAAGAATGCG AGTTTCTTTA AAAAAACCAA GATTCTGAGT 
GACTCAGAAG ACTCTGAATC TGAAGAGCAA GATAGAGAAG ATGGGAAATG TCATAAAATG 
GAAATGAACC CAATTTCAGG AAATCTGAAC TGTGACCCTA TTGCTATGTC CCAGTGTTCC 
TCAGATCATG GATGTGAAAC TGATTTAGAT TCAGATGATG ACAAAATAGA AAAACCAAAC 
AATTTTATGA AAGATTCTGC ATCACAAGAC AATGGACTAA GCAGAAAAAT TTCCAGGAAA 
AGGGTCTGTT CCAGTGACTC AGACAGTAGT TTACAGGTGG TTAAGAAATC ATCAAAAGCC 
AGAACAGGTC TCCTGAGGAT TACTCGAAGA TGTGCAGCTA CGGCTGCCAA TAAGATCAAG 
CTCATGAGTG ATGTAGAAGA TGTCAGTTTA GAAAATGTGC ACACTAGAAG CAAAAATGGA 
AGGAAAAAAC CTCTCCATCT TGCTTGTACT ACAGCTAAGA AGAAATTGAG TGATTGTGAA 
GGAAGTGTAC ATTGTGAAGT ACCAAGTGAA CAGTATGCCT GTGAAGGCAA GCCACCTGAT 
CCTGACTCCG AAGGTAGTAC AAAAGTGCTT AGTCAGGCTC TAAATGGAGA CTCAGACTCT 
GAAGATATGT TGAATTCAGA ACACAAGCAC AGGCATACCA ATATTCACAA AATAGATGCA 
CCTTCTAAAA GAAAAAGTTC CTCTGTTACA TCTTCAGGAG AAGATTCAAA AAGTCATATT 
CCAGGGAGTG AGACTGATAG GACATTTTCT TCAGAGTCAA CCTTGGCACA AAAAGCTACT 
GCAGAGAATA ATTTTGAAGT GGAACTGAAT TATGGGCTGC GCAGGTGGAA TGGCAGAAGA 
CTCAGGACCT ATGGAAAGGC TCCTTTTAGT AAGACAAAAG TGATTCATGA TTCACAGGAA 
ACAGCAGAGA AGGAAGTAAA AAGGAAGAGA TCGCATCCTG AATTGGAAAA TGTGAAAATC 
TCTGAAACAA CTGGGAATTC AAAGTTTAGA CCTGATACTA GTTCCAAATC ATCAGATTTG 
GGATCTGTAA CTGAATCAGA TATTGACTGT ACTGATAATA CAAAAACCAA AAGGAGGAAA 
ACGAAAGGAA AAGCAAAAGT AGTTAGAAAA GAATTTGTTC CTAGAGACAG AGAACCCAAT 
ACAAAAGTGA GAACATGTAT GCATAATCAG AAGGATGCAG TGCAGATGCC TAGTGAAACT 
CTGAAAGCAA AAATGGTACC TGAGAAAGTT CCCCGCAGAT GTGCTACTGT TGCTGCAAAT 
AAAATAAAGA TAATGAGTAA TCTAAAAGAA ACGATCTCAG GACCAGAAAA TGTCTGGATT 
AGGAAGAGTA GCAGAAAACT GCCCCATCGA AATGCTTCTG CTGCGGCTAA GAAAAAATTA 
CTGAATGTTT ATAAAGAGGA TGATACAACC ATAAATTCTG AAAGTGAAAA AGAGCTAGAA 
GATATTAATA GAAAAATGCT TTTTTTAAGG GGGTTCAGAT CCTGGAAAGA AAATGCACAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Trisomy 21 Alters DNA Methylation in Parent-of-Origin-Dependent and -Independent Manners.
PloS one11(4)e0154108(2016)
Alves da Silva AF,Machado FB,Pavarino ?C,Biselli-P?rico JM,Zampieri BL,da Silva Francisco Junior R,Mozer Rodrigues PT,Terra Machado D,Santos-Rebou?as CB,Gomes Fernandes M,Chuva de Sousa Lopes SM,Lopes Rios ?F,Medina-Acosta E

Parent-of-origin-specific allelic associations among 106 genomic loci for age at menarche.
Nature514(7520)92-97(2014 Oct)
Perry JR,Day F,Elks CE,Sulem P,Thompson DJ,Ferreira T,He C,Chasman DI,Esko T,Thorleifsson G,Albrecht E,Ang WQ,Corre T,Cousminer DL,Feenstra B,Franceschini N,Ganna A,Johnson AD,Kjellqvist S,Lunetta KL,McMahon G,Nolte IM,Paternoster L,Porcu E,Smith AV,Stolk L,Teumer A,T?ernikova N,Tikkanen E,Ulivi S,Wagner EK,Amin N,Bierut LJ,Byrne EM,Hottenga JJ,Koller DL,Mangino M,Pers TH,Yerges-Armstrong LM,Zhao JH,Andrulis IL,Anton-Culver H,Atsma F,Bandinelli S,Beckmann MW,Benitez J,Blomqvist C,Bojesen SE,Bolla MK,Bonanni B,Brauch H,Brenner H,Buring JE,Chang-Claude J,Chanock S,Chen J,Chenevix-Trench G,Coll?e JM,Couch FJ,Couper D,Coveillo AD,Cox A,Czene K,D'adamo AP,Smith GD,De Vivo I,Demerath EW,Dennis J,Devilee P,Dieffenbach AK,Dunning AM,Eiriksdottir G,Eriksson JG,Fasching PA,Ferrucci L,Flesch-Janys D,Flyger H,Foroud T,Franke L,Garcia ME,Garc?a-Closas M,Geller F,de Geus EE,Giles GG,Gudbjartsson DF,Gudnason V,Gu?nel P,Guo S,Hall P,Hamann U,Haring R,Hartman CA,Heath AC,Hofman A,Hooning MJ,Hopper JL,Hu FB,Hunter DJ,Karasik D,Kiel DP,Knight JA,Kosma VM,Kutalik Z,Lai S,Lambrechts D,Lindblom A,M?gi R,Magnusson PK,Mannermaa A,Martin NG,Masson G,McArdle PF,McArdle WL,Melbye M,Michailidou K,Mihailov E,Milani L,Milne RL,Nevanlinna H,Neven P,Nohr EA,Oldehinkel AJ,Oostra BA,Palotie A,Peacock M,Pedersen NL,Peterlongo P,Peto J,Pharoah PD,Postma DS,Pouta A,Pylk?s K,Radice P,Ring S,Rivadeneira F,Robino A,Rose LM,Rudolph A,Salomaa V,Sanna S,Schlessinger D,Schmidt MK,Southey MC,Sovio U,Stampfer MJ,St?ckl D,Storniolo AM,Timpson NJ,Tyrer J,Visser JA,Vollenweider P,V?lzke H,Waeber G,Waldenberger M,Wallaschofski H,Wang Q,Willemsen G,Winqvist R,Wolffenbuttel BH,Wright MJ,,,,,,,Boomsma DI,Econs MJ,Khaw KT,Loos RJ,McCarthy MI,Montgomery GW,Rice JP,Streeten EA,Thorsteinsdottir U,van Duijn CM,Alizadeh BZ,Bergmann S,Boerwinkle E,Boyd HA,Crisponi L,Gasparini P,Gieger C,Harris TB,Ingelsson E,J?rvelin MR,Kraft P,Lawlor D,Metspalu A,Pennell CE,Ridker PM,Snieder H,S?rensen TI,Spector TD,Strachan DP,Uitterlinden AG,Wareham NJ,Widen E,Zygmunt M,Murray A,Easton DF,Stefansson K,Murabito JM,Ong KK

A genome-wide search for common SNP x SNP interactions on the risk of venous thrombosis.
BMC medical genetics1436(2013 Mar)
Greliche N,Germain M,Lambert JC,Cohen W,Bertrand M,Dupuis AM,Letenneur L,Lathrop M,Amouyel P,Morange PE,Tr?gou?t DA

Genome-wide association study of temperament in bipolar disorder reveals significant associations with three novel Loci.
Biological psychiatry72(4)303-10(2012 Aug)
Greenwood TA,Akiskal HS,Akiskal KK,,Kelsoe JR

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BRWD1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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